FastQCFastQC Report
Mon 16 Jan 2017
ERR1146489_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146489_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2527049
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110850.4386539398325873No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100660.39833022628370085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51910.20541746519359144No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT26590.10522154497202073No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA67150.060.4180561
GTATCAA126200.043.8211751
CAACGCA156050.034.9237565
ATCAACG157600.034.5802763
TATCAAC162150.033.9011342
TATCACG702.1683572E-433.61712
AACGCAG170400.032.4646226
TCAACGC174800.031.3659424
ACGCAGA180800.030.1788657
GTACTAG13500.029.9826621
CGCAGAG182500.029.8977498
GTGATCG11550.028.4829648
GCAGAGT195200.027.7117969
TGATCGC12300.026.3641099
GTGGTAT16900.025.6216241
GAGTACT139900.024.30468412-13
CAGAGTA192800.023.86408410-11
GTACATG92750.023.6978241
CGTTAAC5800.023.5329041
TACATGG92450.023.2137992
Produced by FastQC (version 0.11.5)