##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146489_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2527049
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.448257631727756	34.0	31.0	34.0	31.0	34.0
2	32.56298314753691	34.0	31.0	34.0	31.0	34.0
3	32.66839503309987	34.0	31.0	34.0	31.0	34.0
4	36.15394992340869	37.0	35.0	37.0	35.0	37.0
5	36.191198508616175	37.0	37.0	37.0	35.0	37.0
6	36.282111664633334	37.0	37.0	37.0	35.0	37.0
7	36.26311480307663	37.0	37.0	37.0	35.0	37.0
8	36.276894907854974	37.0	37.0	37.0	35.0	37.0
9	38.080366862692415	39.0	38.0	39.0	37.0	39.0
10-11	38.048256879862635	39.0	38.0	39.0	36.0	39.0
12-13	38.02757386184439	39.0	38.0	39.0	35.0	39.0
14-15	39.44676379444957	41.0	39.0	41.0	36.5	41.0
16-17	39.43869232452556	41.0	39.0	41.0	36.0	41.0
18-19	39.50663501182605	41.0	39.0	41.0	37.0	41.0
20-21	39.541534414251565	41.0	39.5	41.0	37.0	41.0
22-23	39.49627193615953	41.0	39.0	41.0	36.5	41.0
24-25	39.46814169412623	41.0	39.0	41.0	36.0	41.0
26-27	39.3760605750027	41.0	39.0	41.0	36.0	41.0
28-29	39.28566007228194	41.0	39.0	41.0	35.5	41.0
30-31	39.15501341683521	41.0	39.0	41.0	35.0	41.0
32-33	38.99124789428301	41.0	38.5	41.0	35.0	41.0
34-35	38.88665059521996	40.5	38.0	41.0	35.0	41.0
36-37	38.78064493407132	40.0	38.0	41.0	35.0	41.0
38-39	38.654418454094085	40.0	38.0	41.0	35.0	41.0
40-41	38.53973567588123	40.0	38.0	41.0	35.0	41.0
42-43	38.408593778751424	40.0	38.0	41.0	35.0	41.0
44-45	38.19958833406079	40.0	38.0	41.0	34.0	41.0
46-47	37.88328322877791	40.0	37.5	41.0	33.0	41.0
48-49	37.570676706308426	40.0	37.0	41.0	33.0	41.0
50-51	37.36822356828063	40.0	37.0	41.0	32.5	41.0
52-53	37.21578489376344	40.0	36.0	41.0	32.0	41.0
54-55	37.056394039055036	40.0	36.0	41.0	32.0	41.0
56-57	36.81875301982668	39.0	35.0	41.0	31.5	41.0
58-59	36.57792607108133	39.0	35.0	41.0	31.0	41.0
60-61	36.3788774970331	39.0	35.0	41.0	31.0	41.0
62-63	36.10190918339929	38.0	35.0	41.0	31.0	41.0
64-65	35.802205655687715	37.0	35.0	40.0	30.5	41.0
66-67	35.54622605260128	37.0	35.0	40.0	30.0	41.0
68-69	35.23982914458722	36.0	35.0	39.5	30.0	41.0
70-71	34.98581289876057	36.0	35.0	39.0	30.0	41.0
72-73	34.69243690961275	35.0	35.0	39.0	30.0	41.0
74-75	34.392983673842494	35.0	35.0	37.5	29.0	40.0
76-77	34.117869499166815	35.0	35.0	37.0	29.0	39.0
78-79	33.85683498816208	35.0	34.0	37.0	29.0	39.0
80-81	33.63770033742915	35.0	34.0	36.0	29.0	39.0
82-83	33.411252017669625	35.0	34.0	36.0	29.0	37.0
84-85	33.22494142377136	35.0	34.0	35.5	29.0	37.0
86-87	33.062768470259186	35.0	34.0	35.0	29.0	37.0
88-89	32.93801386518425	35.0	34.0	35.0	29.0	36.0
90-91	32.860881407523166	35.0	34.0	35.0	29.0	36.0
92-93	32.77762283200682	35.0	34.0	35.0	29.0	36.0
94-95	32.71431361243886	35.0	34.0	35.0	29.0	36.0
96-97	32.669504627729815	35.0	34.0	35.0	29.0	35.5
98-99	32.62458306111199	35.0	34.0	35.0	27.5	35.0
100	32.06857009895732	35.0	33.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.37705924837632665
1101	2	0.3552184179121767
1101	3	0.3226535809840527
1101	4	0.18317035499598688
1101	5	0.13725085767761414
1101	6	0.15606159696394428
1101	7	0.19875469971943716
1101	8	0.18039003659652764
1101	9	0.18636098520429556
1101	10-11	0.20090695830969452
1101	12-13	0.22587063223697612
1101	14-15	0.34998976123961256
1101	16-17	0.3690671881252925
1101	18-19	0.31227838299835753
1101	20-21	0.33579585983430604
1101	22-23	0.3948408923365818
1101	24-25	0.2963871763300219
1101	26-27	0.32463937348660465
1101	28-29	0.37282468783595846
1101	30-31	0.3861531632998023
1101	32-33	0.3779914948575538
1101	34-35	0.3631184033283077
1101	36-37	0.4019263439700751
1101	38-39	0.43786164730549615
1101	40-41	0.4226129097550597
1101	42-43	0.35122075077671866
1101	44-45	0.42272625138445363
1101	46-47	0.4601151263801384
1101	48-49	0.5181710467815535
1101	50-51	0.5135303570619385
1101	52-53	0.4007462719606849
1101	54-55	0.4439123484455365
1101	56-57	0.4399677565226767
1101	58-59	0.3333100272748766
1101	60-61	0.3015147900658235
1101	62-63	0.3066770652169737
1101	64-65	0.3204290564271872
1101	66-67	0.28833640586050535
1101	68-69	0.3051685972308036
1101	70-71	0.27287316153527996
1101	72-73	0.23470967631259043
1101	74-75	0.27145365366855856
1101	76-77	0.261151554158495
1101	78-79	0.23862031693858654
1101	80-81	0.2229541984422312
1101	82-83	0.3521527537352469
1101	84-85	0.2838567013644635
1101	86-87	0.350863153476233
1101	88-89	0.30149322004459833
1101	90-91	0.276957271682015
1101	92-93	0.33274982124293473
1101	94-95	0.2866001931457518
1101	96-97	0.2673204937961984
1101	98-99	0.3417702964898268
1101	100	0.3600665669046492
1102	1	0.280420262488974
1102	2	0.28102863405324996
1102	3	0.3199339557497254
1102	4	0.1870566883199274
1102	5	0.13951361504273052
1102	6	0.1912683119361418
1102	7	0.16673581085441924
1102	8	0.20530155540232187
1102	9	0.23871857517306694
1102	10-11	0.2394916967548042
1102	12-13	0.26351359807812
1102	14-15	0.3488598607749225
1102	16-17	0.31342746174933467
1102	18-19	0.33424381359238
1102	20-21	0.2961930727432076
1102	22-23	0.3228787828053683
1102	24-25	0.29242890306982616
1102	26-27	0.36818988921710627
1102	28-29	0.38441386665044774
1102	30-31	0.3588323464642045
1102	32-33	0.34685135724139116
1102	34-35	0.3275962366629912
1102	36-37	0.31087699258252144
1102	38-39	0.24950057307866302
1102	40-41	0.23647477955874763
1102	42-43	0.21699630172329876
1102	44-45	0.2483463588528636
1102	46-47	0.20826149044706455
1102	48-49	0.1675574286895838
1102	50-51	0.18323262624461734
1102	52-53	0.19391762480793773
1102	54-55	0.15377365810320498
1102	56-57	0.2109653486043186
1102	58-59	0.15455667935778195
1102	60-61	0.11178818744606644
1102	62-63	0.19577652897866926
1102	64-65	0.2647843041104778
1102	66-67	0.2733237237366808
1102	68-69	0.2816953351370657
1102	70-71	0.2027927248027126
1102	72-73	0.24514214964504788
1102	74-75	0.23992656788836797
1102	76-77	0.24088282203384637
1102	78-79	0.22550305503957446
1102	80-81	0.13396912903398572
1102	82-83	0.2175301220620085
1102	84-85	0.17070503640825763
1102	86-87	0.22353656173616088
1102	88-89	0.303012630917614
1102	90-91	0.2597236164848056
1102	92-93	0.2277915237399597
1102	94-95	0.24923286566313863
1102	96-97	0.2208476891616158
1102	98-99	0.20389750718928212
1102	100	0.23289666625862537
1103	1	0.2331829802939609
1103	2	0.23741747190978657
1103	3	0.22313427054333346
1103	4	0.1535746879733395
1103	5	0.10233187776249508
1103	6	0.15064417107121386
1103	7	0.14439471582610253
1103	8	0.1171398015224483
1103	9	0.1881203239974809
1103	10-11	0.18459044109835787
1103	12-13	0.23230232023236397
1103	14-15	0.24794056181610813
1103	16-17	0.2380750453566094
1103	18-19	0.20994073093429932
1103	20-21	0.2296189809344895
1103	22-23	0.30950656933465126
1103	24-25	0.2820704214277896
1103	26-27	0.2528683919148733
1103	28-29	0.26191830646506986
1103	30-31	0.23125233533697553
1103	32-33	0.2796007067417676
1103	34-35	0.317859915396312
1103	36-37	0.23513564452059654
1103	38-39	0.31610202691435774
1103	40-41	0.2828876178218138
1103	42-43	0.32812652282150623
1103	44-45	0.30454047028407416
1103	46-47	0.3199786921716523
1103	48-49	0.26530729285666155
1103	50-51	0.18222804896839762
1103	52-53	0.20744391254962125
1103	54-55	0.22592797472356096
1103	56-57	0.18648643234905649
1103	58-59	0.24029396220426236
1103	60-61	0.20000271094939137
1103	62-63	0.1955576327628279
1103	64-65	0.15891345548372726
1103	66-67	0.22125639230304728
1103	68-69	0.1668057669750027
1103	70-71	0.17949917064479592
1103	72-73	0.22305578205836696
1103	74-75	0.17749326282912392
1103	76-77	0.17751432198107153
1103	78-79	0.19699736048058014
1103	80-81	0.3156757898634197
1103	82-83	0.23302973313032993
1103	84-85	0.21359240779044342
1103	86-87	0.2543937619232679
1103	88-89	0.1836327586852562
1103	90-91	0.1940032850655129
1103	92-93	0.11111528036385465
1103	94-95	0.15865250404138465
1103	96-97	0.1857337393487839
1103	98-99	0.18057593952755013
1103	100	0.20156193355305874
1104	1	0.1809914226845848
1104	2	0.163375169924592
1104	3	0.24379762523538062
1104	4	0.14891102587986893
1104	5	0.12703918086382515
1104	6	0.13328529550563673
1104	7	0.1813053728940517
1104	8	0.13474456376321342
1104	9	0.19880528035948686
1104	10-11	0.20659971087093254
1104	12-13	0.16132955239703506
1104	14-15	0.24627673439098885
1104	16-17	0.26803858537691383
1104	18-19	0.23365961957133408
1104	20-21	0.26813565535081096
1104	22-23	0.2747212725996562
1104	24-25	0.1996118424339386
1104	26-27	0.2291087519122641
1104	28-29	0.2806934427559895
1104	30-31	0.22440218586217497
1104	32-33	0.14216676940083062
1104	34-35	0.18913970557071735
1104	36-37	0.20535699335978563
1104	38-39	0.19806668729566468
1104	40-41	0.23028889117706086
1104	42-43	0.18374180684087094
1104	44-45	0.2209694134719271
1104	46-47	0.13598278729619295
1104	48-49	0.12886176998002696
1104	50-51	0.06712168144260033
1104	52-53	0.047505098183464156
1104	54-55	0.002307957687243345
1104	56-57	0.12095069792459157
1104	58-59	0.08986413006800831
1104	60-61	0.06927426865870956
1104	62-63	0.020504757219825365
1104	64-65	0.1371853529026339
1104	66-67	0.028757279916035827
1104	68-69	0.12560798870843826
1104	70-71	0.1101729130580793
1104	72-73	0.050331463668030096
1104	74-75	0.0559247453526055
1104	76-77	0.012425801485676402
1104	78-79	0.04257794380751534
1104	80-81	0.10096550321073039
1104	82-83	0.033711616339680006
1104	84-85	0.06612756058179059
1104	86-87	0.08602264569856288
1104	88-89	0.09139738431159117
1104	90-91	0.10226950572442206
1104	92-93	0.07347790496890383
1104	94-95	0.031154189019886758
1104	96-97	0.10261008600694765
1104	98-99	0.03476639038842677
1104	100	0.02896697705578788
1105	1	0.19157850236305762
1105	2	0.19577367189166495
1105	3	0.2113205478187581
1105	4	0.12125022262549834
1105	5	0.09396140215329751
1105	6	0.110089421903389
1105	7	0.14466997043661678
1105	8	0.14425323548613278
1105	9	0.165484479008569
1105	10-11	0.15110768383360806
1105	12-13	0.2065677238052075
1105	14-15	0.24773198378827033
1105	16-17	0.25212328842865617
1105	18-19	0.27480336862572585
1105	20-21	0.248767127752906
1105	22-23	0.24305737090684687
1105	24-25	0.23261862261326627
1105	26-27	0.24307120840210672
1105	28-29	0.27510231844633637
1105	30-31	0.2384927283526892
1105	32-33	0.25438157158833974
1105	34-35	0.3417651799399266
1105	36-37	0.3736417129444476
1105	38-39	0.3612318154470131
1105	40-41	0.3278917466409723
1105	42-43	0.3426645823277994
1105	44-45	0.36586582978198123
1105	46-47	0.42253326011594794
1105	48-49	0.4993626139044025
1105	50-51	0.43571446489691823
1105	52-53	0.42001655155748097
1105	54-55	0.41683157919122493
1105	56-57	0.420399851951629
1105	58-59	0.4149575446515641
1105	60-61	0.36855769263043214
1105	62-63	0.35212963577866674
1105	64-65	0.27862100173419435
1105	66-67	0.3722438041061764
1105	68-69	0.3298154795305379
1105	70-71	0.3527864800395193
1105	72-73	0.399670936790784
1105	74-75	0.29131702807284654
1105	76-77	0.2689903192661376
1105	78-79	0.2771032907642237
1105	80-81	0.29139354617932156
1105	82-83	0.2564195364002231
1105	84-85	0.2548583738400936
1105	86-87	0.3313351190139713
1105	88-89	0.28131480712664114
1105	90-91	0.29816407478979556
1105	92-93	0.3037157183777097
1105	94-95	0.2780027321949703
1105	96-97	0.25734438913772806
1105	98-99	0.3064510696751128
1105	100	0.2513265994093459
1106	1	0.1389203565370991
1106	2	0.22304843191223966
1106	3	0.20339325995102087
1106	4	0.18299319290945704
1106	5	0.07864393597192532
1106	6	0.06951859908673441
1106	7	0.09167316487229726
1106	8	0.08239887579220806
1106	9	0.14781630701283888
1106	10-11	0.1184993323676764
1106	12-13	0.14108950279289445
1106	14-15	0.1873836268831255
1106	16-17	0.17786705041584128
1106	18-19	0.15992282141626646
1106	20-21	0.1448268845781442
1106	22-23	0.171310164381822
1106	24-25	0.2129935752372063
1106	26-27	0.19564267560051007
1106	28-29	0.258783919603232
1106	30-31	0.24666570173354074
1106	32-33	0.14389240963569705
1106	34-35	0.10512713690813058
1106	36-37	0.21876510628508328
1106	38-39	0.2277893609154944
1106	40-41	0.13297891896016267
1106	42-43	0.09704805130094485
1106	44-45	0.12332965011984243
1106	46-47	0.22949548750290205
1106	48-49	0.2475797657625307
1106	50-51	0.23974040605321534
1106	52-53	0.25349574662825347
1106	54-55	0.23200783983226358
1106	56-57	0.19071586176139022
1106	58-59	0.23548244329131762
1106	60-61	0.18936454571415595
1106	62-63	0.23036270778854373
1106	64-65	0.21389450952220557
1106	66-67	0.1766472939783057
1106	68-69	0.2623065726821068
1106	70-71	0.21120810707199666
1106	72-73	0.2094947416509072
1106	74-75	0.16876940105280624
1106	76-77	0.19210778396531936
1106	78-79	0.1687235283335795
1106	80-81	0.08803555970459342
1106	82-83	0.061962746545702885
1106	84-85	0.12978109182255082
1106	86-87	0.17651181158841212
1106	88-89	0.19963328217568233
1106	90-91	0.19020142377873128
1106	92-93	0.10139845184388463
1106	94-95	0.049053814794490336
1106	96-97	0.10530866158334362
1106	98-99	0.0750185352389039
1106	100	0.014361499702722824
1107	1	0.12151857644965958
1107	2	0.10676008934789394
1107	3	0.11866409763044317
1107	4	0.11239630986462856
1107	5	0.07792831853890902
1107	6	0.058378034653316035
1107	7	0.050495308673681905
1107	8	0.10273732322713158
1107	9	0.09969088901412704
1107	10-11	0.09891167159859293
1107	12-13	0.1393359561068337
1107	14-15	0.18335390997360435
1107	16-17	0.10466010787821745
1107	18-19	0.06830824416973513
1107	20-21	0.10234383480130305
1107	22-23	0.11992802445414696
1107	24-25	0.14679379287868954
1107	26-27	0.09532713549611316
1107	28-29	0.1354024233526161
1107	30-31	0.15310454749825197
1107	32-33	0.12454463507265956
1107	34-35	0.09751518242716628
1107	36-37	0.09733593934080886
1107	38-39	0.04757034324386211
1107	40-41	0.06536992801495956
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2216	40-41	-0.4548351308521319
2216	42-43	-0.48969089091549733
2216	44-45	-0.5004477599756569
2216	46-47	-0.49288502870985695
2216	48-49	-0.46592886488117813
2216	50-51	-0.41489979384310516
2216	52-53	-0.41947891948791494
2216	54-55	-0.38595452110548933
2216	56-57	-0.3945168292701169
2216	58-59	-0.3727656001960895
2216	60-61	-0.40518413714784884
2216	62-63	-0.3243132202962471
2216	64-65	-0.29547588582171613
2216	66-67	-0.3517526447993049
2216	68-69	-0.40911396261896726
2216	70-71	-0.40417842620338007
2216	72-73	-0.4368060350088854
2216	74-75	-0.42360101391950167
2216	76-77	-0.4393231623188498
2216	78-79	-0.40813479413890263
2216	80-81	-0.3628742610542588
2216	82-83	-0.33464340457988584
2216	84-85	-0.2956429237149649
2216	86-87	-0.3353919210825893
2216	88-89	-0.29887152887621227
2216	90-91	-0.25405429134155355
2216	92-93	-0.3678827943516936
2216	94-95	-0.29753080184341485
2216	96-97	-0.2892695829792231
2216	98-99	-0.3335430290589585
2216	100	-0.5678759063377541
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	11.0
8	73.0
9	107.0
10	229.0
11	258.0
12	174.0
13	217.0
14	305.0
15	611.0
16	1243.0
17	2401.0
18	4492.0
19	6955.0
20	9771.0
21	14782.0
22	21766.0
23	25769.0
24	24457.0
25	19439.0
26	15860.0
27	16142.0
28	18491.0
29	20573.0
30	25820.0
31	32420.0
32	41995.0
33	56346.0
34	84450.0
35	137966.0
36	256662.0
37	635878.0
38	766325.0
39	284431.0
40	630.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.3564614177298	20.618236027637035	13.841471305343031	23.183831249290137
2	15.627061935084244	21.501472909786948	39.369702336773955	23.50176281835486
3	17.502763113063857	27.59947305959816	31.247375883493778	23.650387943844205
4	12.428488723408213	18.486068137182933	39.114477004601014	29.97096613480783
5	12.533639370117392	38.106132163236154	35.21146386979238	14.148764596854072
6	28.12156578593813	39.20788646916197	18.261658965057237	14.408888779842666
7	24.40881566857113	34.32521608233487	23.660075835580358	17.605892413513644
8	25.561198061454288	33.55965792511345	21.28644913493961	19.59269487849266
9	26.535654829012024	14.222161897137727	21.606347957637546	37.635835316212706
10-11	24.365158728619825	26.219732977081172	28.296048078212966	21.119060216086037
12-13	25.74121039995663	24.561336167205305	28.08491248092142	21.612540951916642
14-15	22.91728868678223	24.95622858049618	27.149854860324208	24.976627872397383
16-17	21.16516141950552	27.75949338536768	27.95220828721564	23.123136907911164
18-19	20.924643724755633	26.46129932581442	31.23732464230017	21.376732307129778
20-21	22.32659517088905	25.536346940641042	30.787669728604396	21.34938815986552
22-23	22.59849725221727	25.353838239440453	30.40673120204983	21.64093330629245
24-25	22.13358348017787	25.59204431730449	30.787946731543393	21.486425470974247
26-27	21.90626299687897	26.00082151157338	30.321018706008473	21.77189678553918
28-29	21.377503958174138	26.12800543242335	30.714382665314368	21.780107944088144
30-31	21.93454767632795	25.67789143487919	30.647251899500823	21.74030898929204
32-33	22.174528482949963	26.073297103327537	29.863993038393705	21.8881813753288
34-35	22.223068632651653	25.911536215802737	30.49172906415345	21.373666087392163
36-37	22.2693570729416	25.687007487252323	30.100550903956957	21.94308453584912
38-39	22.15517608103576	25.622246647005927	30.385303710973243	21.837273560985068
40-41	22.072066902702915	25.29901903947467	30.46733435878644	22.161579699035975
42-43	22.19439203888355	25.511701966634774	29.90814324706944	22.385762747412233
44-45	22.1262292558439	26.144868476401413	29.629443494495728	22.09945877325896
46-47	22.546655375459036	25.89832373053058	28.774218057490188	22.7808028365202
48-49	23.031329985010107	25.965063418170537	28.49541913926038	22.50818745755897
50-51	22.782970522708474	26.24873863784759	28.01539752199222	22.95289331745171
52-53	22.486186806805243	26.614108105487784	27.921892266491493	22.977812821215483
54-55	22.40651995836977	26.62690294062944	27.81225638204835	23.154320718952437
56-57	23.022853741908474	26.32224599192885	27.666434773524294	22.988465492638383
58-59	22.416162359652272	26.41051135098878	28.267271340422816	22.906054948936134
60-61	22.539159050922795	26.824335714811465	27.861315503509694	22.77518973075605
62-63	22.733533239217298	26.54685504383123	27.517602237415772	23.202009479535697
64-65	21.994159732387683	26.62513816122985	27.79760413686282	23.583097969519645
66-67	22.613547368141955	26.309003653033454	27.784641588386904	23.292807390437687
68-69	22.31790177381578	26.148680614819387	28.055185819604517	23.478231791760315
70-71	22.45663000866836	26.04900303976266	27.87440396374129	23.61996298782769
72-73	22.87977281509944	26.09191356225552	27.892023854256266	23.136289768388778
74-75	22.77057303219163	26.576686383367214	27.58172761669056	23.07101296775059
76-77	22.806633962104364	26.30590868537434	27.264805449538017	23.62265190298328
78-79	22.598245844525664	26.518404228208	27.312162746119157	23.57118718114718
80-81	22.461755254602554	26.53936126620543	27.59068738940894	23.408196089783072
82-83	22.91418971284846	25.806717765865315	28.21696324818845	23.062129273097774
84-85	23.167095197062977	26.307360664965735	27.135896733243044	23.389647404728244
86-87	22.303663284724593	26.641746954649477	27.493451848381255	23.56113791224468
88-89	22.629623679189063	26.100395481522693	27.42680584366094	23.843174995627304
90-91	22.609870247866187	26.028719664715645	27.57283297632931	23.788577111088863
92-93	22.893996119584543	26.105983698772757	27.406373995913814	23.593646185728886
94-95	22.117062233458867	26.091500402247842	27.82348106427695	23.967956300016343
96-97	22.412530213166054	26.037734206448327	27.560600448587362	23.98913513179826
98-99	22.27484860242533	26.38060322389231	27.38203236713647	23.962515806545884
100	22.715111472888893	25.80000134545456	26.971377828570887	24.51350935308566
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	343.0
1	229.0
2	285.0
3	427.0
4	262.5
5	195.0
6	453.0
7	1132.5
8	2135.5
9	2672.5
10	1978.0
11	1208.0
12	1207.5
13	1375.0
14	1662.0
15	2079.5
16	2551.5
17	3180.0
18	3920.0
19	4569.5
20	5271.5
21	6161.0
22	7207.5
23	8207.0
24	9282.0
25	10653.0
26	13388.5
27	16313.0
28	18711.0
29	22717.5
30	27901.5
31	32961.5
32	39298.5
33	45246.5
34	50552.5
35	54560.5
36	59047.5
37	67795.5
38	71598.0
39	72305.5
40	71334.5
41	68440.0
42	68545.5
43	71062.0
44	76741.0
45	86838.5
46	95315.0
47	102488.5
48	106407.5
49	102787.0
50	99882.5
51	101492.0
52	101018.0
53	98385.5
54	96725.0
55	88352.5
56	79577.0
57	68519.0
58	56613.5
59	51336.5
60	42761.0
61	32426.0
62	24910.5
63	18795.0
64	13377.0
65	8667.0
66	5797.0
67	3965.5
68	2835.0
69	2042.0
70	1690.0
71	1384.0
72	1031.0
73	904.0
74	761.0
75	629.5
76	533.0
77	433.0
78	353.5
79	275.5
80	211.0
81	163.5
82	123.5
83	86.0
84	59.0
85	35.0
86	23.0
87	19.0
88	10.5
89	6.0
90	1.0
91	0.5
92	1.5
93	2.0
94	1.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.00593577726431106
2	0.08369445942678595
3	3.165747874299232E-4
4	0.0
5	0.03921570179288174
6	0.003482322661729155
7	1.187155452862212E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	1.97859242143702E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.550762569305146E-4
24-25	0.0
26-27	0.0
28-29	0.0
30-31	1.3850146950059142E-4
32-33	0.0018203050277220583
34-35	0.001483944316077765
36-37	8.112228927891781E-4
38-39	6.529354990742166E-4
40-41	0.0014245865434346545
42-43	6.529354990742166E-4
44-45	4.748621811448848E-4
46-47	3.5614663585866363E-4
48-49	5.144340295736253E-4
50-51	7.518651201460676E-4
52-53	0.0010486539833616207
54-55	0.0015433020887208758
56-57	9.101525138610291E-4
58-59	0.0026315279205112365
60-61	0.0018598768761507987
62-63	0.0010486539833616207
64-65	0.004352903327161445
66-67	0.004273759630303964
68-69	0.0037791115249447083
70-71	0.0016818035582214668
72-73	0.004768407735663218
74-75	0.003482322661729155
76-77	0.003264677495371083
78-79	8.310088170035483E-4
80-81	9.892962107185101E-5
82-83	1.3850146950059142E-4
84-85	5.93577726431106E-5
86-87	0.0
88-89	1.187155452862212E-4
90-91	0.0
92-93	0.0
94-95	0.0
96-97	1.9785924214370203E-4
98-99	4.1550440850177415E-4
100	8.705806654322887E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2527049.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.461279092207505
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.11408159525736	30.252835178300234
2	15.99202836187145	14.860201859428148
3	7.2079033508756565	10.046652277640746
4	3.8663698659618806	7.185459576646235
5	2.243662598362077	5.212171708562396
6	1.453362887700025	4.0515059248612495
7	0.9761629213462788	3.1747644549693845
8	0.6644695348289842	2.4697683604766976
9	0.47681808709773166	1.9938220398774193
>10	1.9344030967759445	14.150217936541775
>50	0.04377265245875209	1.3698819188199791
>100	0.023504376344226887	2.0887259201639408
>500	0.0020187248197893193	0.6679368546486433
>1k	0.0010093624098946596	0.7112824057978582
>5k	1.4419462998495138E-4	0.3477693292358182
>10k+	2.8838925996990276E-4	1.4170042540294339
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11085	0.4386539398325873	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10066	0.39833022628370085	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5191	0.20541746519359144	No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT	2659	0.10522154497202073	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	7.91436968574808E-5	0.0
22-23	0.0	0.0	0.0	4.748621811448848E-4	0.0
24-25	0.0	0.0	0.0	0.0013454428465771735	0.0
26-27	0.0	0.0	0.0	0.0027700293900118278	0.0
28-29	0.0	0.0	0.0	0.007479079353031935	0.0
30-31	0.0	0.0	0.0	0.02073564857665997	0.0
32-33	0.0	0.0	0.0	0.04695199816070049	0.0
34-35	0.0	0.0	0.0	0.08933344782788145	0.0
36-37	0.0	0.0	0.0	0.15266819123808045	0.0
38-39	0.0	0.0	0.0	0.23808402607151663	0.0
40-41	0.0	0.0	0.0	0.32692282579403886	0.0
42-43	0.0	0.0	0.0	0.4167311358030652	0.0
44-45	0.0	0.0	0.0	0.5241291324386665	0.0
46-47	0.0	0.0	0.0	0.6520847043329987	0.0
48-49	0.0	0.0	0.0	0.7859958394158562	0.0
50-51	0.0	0.0	0.0	0.9187593908942803	0.0
52-53	0.0	0.0	0.0	1.0452310184725346	0.0
54-55	0.0	0.0	0.0	1.1829608369287656	0.0
56-57	0.0	0.0	0.0	1.3445920518359555	0.0
58-59	0.0	0.0	0.0	1.5187477567708423	0.0
60-61	0.0	0.0	0.0	1.698641379727896	0.0
62-63	0.0	0.0	0.0	1.8767938413540852	0.0
64-65	0.0	0.0	0.0	2.0586264848841473	0.0
66-67	0.0	0.0	0.0	2.263410800502879	0.0
68-69	0.0	0.0	0.0	2.484914222082754	0.0
70-71	0.0	0.0	0.0	2.71923892255354	0.0
72-73	0.0	0.0	0.0	2.95312833269161	0.0
74-75	0.0	0.0	0.0	3.19141021800527	0.0
76-77	0.0	0.0	0.0	3.4531977812856023	0.0
78-79	0.0	0.0	0.0	3.7381942336693905	0.0
80-81	0.0	0.0	0.0	4.043055753964406	0.0
82-83	0.0	0.0	0.0	4.3539717670690194	0.0
84-85	0.0	0.0	0.0	4.660040228741113	0.0
86-87	0.0	0.0	0.0	4.983737948888209	0.0
88	0.0	0.0	0.0	5.238679582390369	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6715	0.0	60.418056	1
GTATCAA	12620	0.0	43.821175	1
CAACGCA	15605	0.0	34.923756	5
ATCAACG	15760	0.0	34.580276	3
TATCAAC	16215	0.0	33.901134	2
TATCACG	70	2.1683572E-4	33.6171	2
AACGCAG	17040	0.0	32.464622	6
TCAACGC	17480	0.0	31.365942	4
ACGCAGA	18080	0.0	30.178865	7
GTACTAG	1350	0.0	29.982662	1
CGCAGAG	18250	0.0	29.897749	8
GTGATCG	1155	0.0	28.482964	8
GCAGAGT	19520	0.0	27.711796	9
TGATCGC	1230	0.0	26.364109	9
GTGGTAT	1690	0.0	25.621624	1
GAGTACT	13990	0.0	24.304684	12-13
CAGAGTA	19280	0.0	23.864084	10-11
GTACATG	9275	0.0	23.697824	1
CGTTAAC	580	0.0	23.532904	1
TACATGG	9245	0.0	23.213799	2
>>END_MODULE