FastQCFastQC Report
Mon 16 Jan 2017
ERR1146488_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146488_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567973
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37400.030.296271
GTATCAA55650.029.738651
GCGTAAT804.7731106E-429.3848691
GTACTAG10300.027.387841
CAACGCA59500.026.142275
ATCAACG59700.025.9764823
AACGCAG65250.024.2701916
TATCAAC67850.023.6873782
TCAACGC66650.023.338724
GTACATG88300.023.2682911
ACATGGG80900.023.1193313
TACATGG86950.022.6997172
GCATCGT8750.022.5552758
TATTCGC2103.868263E-822.3762659
TCTAACG4050.022.0464842
CGCAGAG70750.021.6528
ACGCAGA72000.021.4710547
TAACGCC4550.020.6570244
CGCGGTA1150.00395671220.43766694
CTAACGC4450.020.0647753
Produced by FastQC (version 0.11.5)