##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146488_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2567973
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15746583005351	31.0	31.0	34.0	28.0	34.0
2	31.38987832037175	31.0	31.0	34.0	28.0	34.0
3	31.61037129284459	33.0	31.0	34.0	28.0	34.0
4	35.213717200297665	37.0	35.0	37.0	32.0	37.0
5	35.350039895279274	37.0	35.0	37.0	33.0	37.0
6	35.339629739097724	37.0	35.0	37.0	33.0	37.0
7	35.24695625693884	37.0	35.0	37.0	32.0	37.0
8	35.2373081804209	37.0	35.0	37.0	33.0	37.0
9	36.88697622599614	39.0	37.0	39.0	33.0	39.0
10-11	36.874365696212536	39.0	37.5	39.0	33.0	39.0
12-13	36.84173256494519	39.0	38.0	39.0	33.0	39.0
14-15	38.26649832377521	40.0	39.0	41.0	33.0	41.0
16-17	38.28561067425553	40.5	39.0	41.0	33.0	41.0
18-19	38.308493703010114	40.0	38.5	41.0	33.5	41.0
20-21	38.40513237483416	41.0	39.0	41.0	34.0	41.0
22-23	38.4374940858023	40.5	39.0	41.0	34.0	41.0
24-25	38.49864757145032	41.0	39.0	41.0	34.0	41.0
26-27	38.53185781158914	41.0	39.0	41.0	34.0	41.0
28-29	38.509390675057716	40.0	39.0	41.0	33.0	41.0
30-31	38.498799052793785	40.0	39.0	41.0	33.0	41.0
32-33	38.51205970623523	40.0	38.5	41.0	33.0	41.0
34-35	38.4656119826805	40.0	38.0	41.0	33.0	41.0
36-37	38.37926294396397	40.0	38.0	41.0	33.0	41.0
38-39	38.33848798254499	40.0	38.0	41.0	33.0	41.0
40-41	38.28198310496255	40.0	38.0	41.0	33.0	41.0
42-43	38.17900986497911	40.0	38.0	41.0	33.0	41.0
44-45	38.05676773081337	40.0	38.0	41.0	33.0	41.0
46-47	37.96638905471358	40.0	38.0	41.0	32.5	41.0
48-49	37.858128181254244	40.0	37.5	41.0	32.0	41.0
50-51	37.74861417935469	40.0	37.0	41.0	32.0	41.0
52-53	37.640344544120985	40.0	37.0	41.0	32.0	41.0
54-55	37.47693238986547	40.0	36.5	41.0	31.0	41.0
56-57	37.363445799469076	40.0	36.0	41.0	31.0	41.0
58-59	37.158659573134145	39.5	35.5	41.0	31.0	41.0
60-61	36.99304977116192	39.0	35.0	41.0	31.0	41.0
62-63	36.767687588615615	39.0	35.0	41.0	31.0	41.0
64-65	36.5293007364174	38.5	35.0	40.5	31.0	41.0
66-67	36.27544156422205	37.5	35.0	40.0	30.5	41.0
68-69	35.981905572994734	37.0	35.0	40.0	30.0	41.0
70-71	35.71447674878202	37.0	35.0	39.5	30.0	41.0
72-73	35.3887435732385	36.0	35.0	39.0	30.0	41.0
74-75	35.06188188115685	36.0	34.5	39.0	30.0	40.5
76-77	34.73690513880014	35.0	34.0	37.5	29.5	40.0
78-79	34.43704606707313	35.0	34.0	37.0	29.0	39.0
80-81	34.138805976542585	35.0	34.0	37.0	29.0	39.0
82-83	33.862271332292046	35.0	34.0	36.0	29.0	38.5
84-85	33.61342681562462	35.0	34.0	36.0	29.0	37.0
86-87	33.39427400521734	35.0	34.0	36.0	29.0	37.0
88-89	33.23546080897268	35.0	34.0	35.0	28.5	36.5
90-91	33.06281978042604	35.0	34.0	35.0	28.0	36.0
92-93	32.96018143492942	35.0	34.0	35.0	28.0	36.0
94-95	32.85511452028506	35.0	34.0	35.0	28.0	36.0
96-97	32.772348657871404	35.0	34.0	35.0	27.0	35.5
98-99	32.69700947011515	35.0	33.5	35.0	27.0	35.0
100	31.809271359161485	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.24327060170801929
1101	2	0.169436166269751
1101	3	0.2623130407051484
1101	4	0.277264042155025
1101	5	0.26744758441624583
1101	6	0.2608171367834444
1101	7	0.2893585358451318
1101	8	0.3210599395554965
1101	9	0.44059088898627863
1101	10-11	0.4432225211805232
1101	12-13	0.3321864559726109
1101	14-15	0.5080477219252302
1101	16-17	0.4414333216616626
1101	18-19	0.4245742856879602
1101	20-21	0.3737840568173354
1101	22-23	0.3850087755530538
1101	24-25	0.3704029853741062
1101	26-27	0.3760460613932253
1101	28-29	0.4942075883016841
1101	30-31	0.4619554875888099
1101	32-33	0.4309843567089615
1101	34-35	0.4455431261323781
1101	36-37	0.5280837228538431
1101	38-39	0.49305964827168935
1101	40-41	0.3994549205989131
1101	42-43	0.42709373840157383
1101	44-45	0.3567554373368438
1101	46-47	0.30599473181163717
1101	48-49	0.37466129097942513
1101	50-51	0.29414071419257226
1101	52-53	0.3141783717447808
1101	54-55	0.33823218304620895
1101	56-57	0.27712710902005
1101	58-59	0.3442834403092334
1101	60-61	0.2734707926241384
1101	62-63	0.2682546010229032
1101	64-65	0.235537403298963
1101	66-67	0.2449768910763197
1101	68-69	0.21568567654254878
1101	70-71	0.1433371056131847
1101	72-73	0.20936800776681253
1101	74-75	0.1803398299946366
1101	76-77	0.2411020584465362
1101	78-79	0.1446529442348634
1101	80-81	0.19801440363139022
1101	82-83	0.2693972692447488
1101	84-85	0.2131010795724677
1101	86-87	0.19402503461898135
1101	88-89	0.16565211341391972
1101	90-91	0.2530163020835232
1101	92-93	0.19470242609729382
1101	94-95	0.19675086048410861
1101	96-97	0.19565125462987254
1101	98-99	0.17508688665955674
1101	100	0.15772128393482276
1102	1	0.11629828742193027
1102	2	0.10297385747845667
1102	3	0.17959040000099336
1102	4	0.3247978708815822
1102	5	0.2793790937087337
1102	6	0.4040807372932633
1102	7	0.3427833264720377
1102	8	0.37931700603166263
1102	9	0.480752729792151
1102	10-11	0.5200025824554757
1102	12-13	0.4994472644360002
1102	14-15	0.5368796772109974
1102	16-17	0.5803986892169561
1102	18-19	0.5269133295514266
1102	20-21	0.5632459939137959
1102	22-23	0.5250857026878251
1102	24-25	0.5157005353079853
1102	26-27	0.47097442444743365
1102	28-29	0.49772779032475256
1102	30-31	0.4694918493827984
1102	32-33	0.43837328036518386
1102	34-35	0.5025223143823183
1102	36-37	0.5178752423288415
1102	38-39	0.4943097324439165
1102	40-41	0.5267987271315562
1102	42-43	0.5194896012525803
1102	44-45	0.38020507001370163
1102	46-47	0.40487779169227167
1102	48-49	0.4662208607274678
1102	50-51	0.5097165830869628
1102	52-53	0.42686725685396
1102	54-55	0.39608546593320426
1102	56-57	0.462714611711327
1102	58-59	0.42036702958960603
1102	60-61	0.3914270156115407
1102	62-63	0.3684731935595664
1102	64-65	0.4386395762672777
1102	66-67	0.4049418819127908
1102	68-69	0.39980118830684575
1102	70-71	0.41514253362176134
1102	72-73	0.4603990415702981
1102	74-75	0.31684907022107467
1102	76-77	0.3836646491570477
1102	78-79	0.3597998690334734
1102	80-81	0.32308558031413526
1102	82-83	0.2809452530049157
1102	84-85	0.3137514928489793
1102	86-87	0.31601431259671386
1102	88-89	0.3530105349190862
1102	90-91	0.36244923126321993
1102	92-93	0.27456321110996385
1102	94-95	0.2946262035846914
1102	96-97	0.3191774303487378
1102	98-99	0.26637297264009874
1102	100	0.18725850321183657
1103	1	0.08514051925821065
1103	2	0.05782693127749994
1103	3	0.13316302194911955
1103	4	0.1463523699288487
1103	5	0.19675653477717248
1103	6	0.19077352346371867
1103	7	0.1920594444122159
1103	8	0.1066364926213339
1103	9	0.2785765924100545
1103	10-11	0.22563243470089844
1103	12-13	0.197493132190246
1103	14-15	0.20031070850689758
1103	16-17	0.2377109060466296
1103	18-19	0.1741604618686452
1103	20-21	0.2353016113703248
1103	22-23	0.19647825894862336
1103	24-25	0.18788877298801765
1103	26-27	0.19680670417789514
1103	28-29	0.21027752789878917
1103	30-31	0.27511874741492903
1103	32-33	0.28949961410265246
1103	34-35	0.2384908591967303
1103	36-37	0.2559154491716811
1103	38-39	0.26204173898949534
1103	40-41	0.32507618125441695
1103	42-43	0.2624294107537324
1103	44-45	0.1264456539578731
1103	46-47	0.09227399999943486
1103	48-49	0.19482319447612184
1103	50-51	0.20511910861994664
1103	52-53	0.16292838032530454
1103	54-55	0.14579617260098843
1103	56-57	0.15375188789474947
1103	58-59	0.1456781701275176
1103	60-61	0.09525108502437263
1103	62-63	0.09472680797964017
1103	64-65	0.12908549080987797
1103	66-67	0.08026693519842354
1103	68-69	0.11538218824416901
1103	70-71	0.0337673313679403
1103	72-73	0.12033004871349817
1103	74-75	0.06865387496953446
1103	76-77	0.06580607054084453
1103	78-79	0.10834781332020071
1103	80-81	0.1367984395654318
1103	82-83	0.11112892921994444
1103	84-85	0.06893388158592728
1103	86-87	0.03637610791205503
1103	88-89	-0.04102814212032513
1103	90-91	0.062174130131317895
1103	92-93	0.06873418899846939
1103	94-95	0.08203482757202352
1103	96-97	-0.00433651054383688
1103	98-99	0.06753198237183966
1103	100	0.03570760111885818
1104	1	0.13830388245925462
1104	2	0.08934355120124238
1104	3	0.17094598594449906
1104	4	0.20917508657272776
1104	5	0.21226831198494267
1104	6	0.15287752738458948
1104	7	0.14750789787782992
1104	8	0.21272525275185927
1104	9	0.2975065477695793
1104	10-11	0.3617133978161249
1104	12-13	0.3624242648725513
1104	14-15	0.3912701886654446
1104	16-17	0.3812206015233386
1104	18-19	0.30999860689919245
1104	20-21	0.3204391053102924
1104	22-23	0.259930346725497
1104	24-25	0.30949639852205024
1104	26-27	0.2981306805874624
1104	28-29	0.2268305875923886
1104	30-31	0.20052522890658508
1104	32-33	0.2307153116891456
1104	34-35	0.2592095036911246
1104	36-37	0.32434026634740576
1104	38-39	0.25102103501415485
1104	40-41	0.25218599029388145
1104	42-43	0.20814107129056225
1104	44-45	0.137427581333057
1104	46-47	0.13926991272593625
1104	48-49	0.12402591649490091
1104	50-51	0.11233163205932328
1104	52-53	0.039948336060092515
1104	54-55	0.0967527947383715
1104	56-57	0.13096470320100195
1104	58-59	0.17220037911254593
1104	60-61	0.04946225033512519
1104	62-63	0.0804454202549536
1104	64-65	0.09237477751374712
1104	66-67	0.09407417190512035
1104	68-69	0.0915084627453524
1104	70-71	0.12356954038328638
1104	72-73	0.09754002116051197
1104	74-75	0.05441581241981197
1104	76-77	-3.2774322306750037E-4
1104	78-79	0.023717788708616183
1104	80-81	0.12508934669907745
1104	82-83	0.11504479726427519
1104	84-85	0.05138597277423429
1104	86-87	0.15352040651992382
1104	88-89	0.07635961895365284
1104	90-91	0.08274521127827228
1104	92-93	0.05215861385961773
1104	94-95	0.05652604105217307
1104	96-97	0.018113624757312152
1104	98-99	-0.050691579321430424
1104	100	0.011799079022829062
1105	1	0.0184382961866838
1105	2	0.001110932276265686
1105	3	0.07123008471264569
1105	4	0.07894429374806577
1105	5	0.0499829799341569
1105	6	-0.0058341865035131946
1105	7	-0.03561871612980383
1105	8	0.09276643423692121
1105	9	0.15456978618263406
1105	10-11	0.13236972648972056
1105	12-13	0.14028831348871762
1105	14-15	0.19358558402527137
1105	16-17	0.10845427999613122
1105	18-19	0.11822511132516667
1105	20-21	0.1433496879093994
1105	22-23	0.11817264231807911
1105	24-25	0.24177878426330324
1105	26-27	0.22932222775839506
1105	28-29	0.21485422690835065
1105	30-31	0.2225801656676012
1105	32-33	0.18790450252359392
1105	34-35	0.19746120642406595
1105	36-37	0.18656456008116606
1105	38-39	0.1803389056864546
1105	40-41	0.19041827853241244
1105	42-43	0.21426157954638114
1105	44-45	0.16802429115228534
1105	46-47	0.17215774623120694
1105	48-49	0.07083930458801291
1105	50-51	0.04329425887313221
1105	52-53	0.06424539802127072
1105	54-55	0.14842770735003086
1105	56-57	0.08481211195981331
1105	58-59	0.14924341507295225
1105	60-61	0.1341247357502482
1105	62-63	0.19234612130310325
1105	64-65	0.17750719325501763
1105	66-67	0.10388602980893324
1105	68-69	0.16238658601658074
1105	70-71	0.1247245961274217
1105	72-73	0.11899552651730971
1105	74-75	0.11746687204229289
1105	76-77	0.11703920473093632
1105	78-79	0.07191960472478343
1105	80-81	0.05708869329111366
1105	82-83	0.07560606531948366
1105	84-85	0.12752289943416173
1105	86-87	0.07765563835850031
1105	88-89	0.050410440410779245
1105	90-91	0.041929970345464085
1105	92-93	0.10974193315359315
1105	94-95	0.03349977509024171
1105	96-97	0.01954343471074793
1105	98-99	0.011471711652987437
1105	100	-0.08532232037961762
1106	1	0.12272665667509486
1106	2	0.08902287214904092
1106	3	0.1294072290819024
1106	4	0.148676477415151
1106	5	0.17012931150679123
1106	6	0.12009413233298716
1106	7	0.13518109004444057
1106	8	0.18836858281604663
1106	9	0.12009327037242912
1106	10-11	0.1822889657579907
1106	12-13	0.12608177908636975
1106	14-15	0.21810419851477292
1106	16-17	0.2698675928013472
1106	18-19	0.1675339712929329
1106	20-21	0.2204771833300967
1106	22-23	0.18204362447166744
1106	24-25	0.2390363560114821
1106	26-27	0.2185807411423326
1106	28-29	0.24824631510593775
1106	30-31	0.22458516252309124
1106	32-33	0.12757207760530775
1106	34-35	0.15595720103543442
1106	36-37	0.1877508493145541
1106	38-39	0.19249930680954463
1106	40-41	0.16290832090602692
1106	42-43	0.15627001302875243
1106	44-45	0.13037930845570145
1106	46-47	0.08185398278111222
1106	48-49	0.09364080845593747
1106	50-51	0.16526894467951792
1106	52-53	0.0774566165460655
1106	54-55	0.16072079663702965
1106	56-57	0.08287705046137006
1106	58-59	0.10008074921275778
1106	60-61	0.09257107110317264
1106	62-63	0.08522412448541417
1106	64-65	0.07382254501096241
1106	66-67	0.07057301839589769
1106	68-69	0.05645448689051591
1106	70-71	0.07756452148389315
1106	72-73	0.10908666586222182
1106	74-75	0.09771327818972964
1106	76-77	0.1687870387968431
1106	78-79	0.12331676538365599
1106	80-81	0.06411439582356593
1106	82-83	0.05190540239416208
1106	84-85	0.11840597507776351
1106	86-87	0.07772151849582798
1106	88-89	0.05960417337799839
1106	90-91	0.051244706945198004
1106	92-93	0.08921795065289473
1106	94-95	0.06736976062205002
1106	96-97	0.042334491232949745
1106	98-99	0.023612249072698432
1106	100	-0.015481745500672162
1107	1	-0.00653826758982845
1107	2	0.031585174711342034
1107	3	0.04325783793251148
1107	4	0.0688287575674309
1107	5	0.07270146907894315
1107	6	0.03328567052452058
1107	7	-0.0202752104118602
1107	8	0.103881448328913
1107	9	0.06787508315424162
1107	10-11	0.10060105907008676
1107	12-13	0.0952842657888553
1107	14-15	0.2114908794014596
1107	16-17	0.08449431742806723
1107	18-19	0.06593124969021602
1107	20-21	0.09088387123679098
1107	22-23	0.06023995516800085
1107	24-25	0.08273319970832205
1107	26-27	0.08801056807216412
1107	28-29	0.1406803675399928
1107	30-31	0.20457969501762108
1107	32-33	0.18626176629499014
1107	34-35	0.1308286809069088
1107	36-37	0.14323360979732058
1107	38-39	0.1474442267544589
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2216	32-33	-0.3028875577712
2216	34-35	-0.3209981001954034
2216	36-37	-0.2574417965194229
2216	38-39	-0.332334268166548
2216	40-41	-0.3745698541255962
2216	42-43	-0.24324443926479944
2216	44-45	-0.23635882277173437
2216	46-47	-0.17421104482785665
2216	48-49	-0.12438214246487433
2216	50-51	-0.2247406182041587
2216	52-53	-0.11533605396632396
2216	54-55	-0.15475935266734808
2216	56-57	-0.13293779977152553
2216	58-59	-0.07615394246136731
2216	60-61	-0.11804095345183896
2216	62-63	-0.1113758076489546
2216	64-65	-0.10088424463643975
2216	66-67	-0.13020188366166252
2216	68-69	-0.17254387816485206
2216	70-71	-0.16307403236132245
2216	72-73	-0.1550898464615571
2216	74-75	-0.16942927047301026
2216	76-77	-0.1340404899689247
2216	78-79	-0.17207762644779478
2216	80-81	-0.09480345592089634
2216	82-83	-0.12607189346912406
2216	84-85	-0.07701216972280633
2216	86-87	-0.08428699555543773
2216	88-89	-0.17896756367354527
2216	90-91	-0.10817997763388121
2216	92-93	-0.10429563789632113
2216	94-95	-0.13357141221936075
2216	96-97	-0.13788545509151362
2216	98-99	-0.1425138575997238
2216	100	-0.11800758071059292
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	67.0
1	33.0
2	37.0
3	40.0
4	19.0
5	31.0
6	100.0
7	479.0
8	1388.0
9	1699.0
10	1682.0
11	2009.0
12	2611.0
13	3221.0
14	4233.0
15	5027.0
16	5228.0
17	5899.0
18	6479.0
19	7238.0
20	8335.0
21	9494.0
22	10713.0
23	12549.0
24	15327.0
25	20032.0
26	22827.0
27	21249.0
28	21393.0
29	27543.0
30	32159.0
31	34003.0
32	43230.0
33	58626.0
34	88255.0
35	136780.0
36	258984.0
37	593581.0
38	816884.0
39	287913.0
40	576.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.11273609423742	20.47739799739251	13.376510936880226	24.033354971489846
2	14.817787623095683	21.30249505592125	40.70361309146877	23.176104229514298
3	17.796514125413747	26.919351616266376	30.739730892943314	24.544403365376564
4	11.99409904736535	18.040472465661182	40.463430411637404	29.50199807533607
5	12.789524764999674	38.757138806756046	35.76931645049025	12.68401997775403
6	28.795535836687897	39.30086990117025	19.112316018395045	12.791278243746806
7	25.655544110860802	33.41120485376211	23.883476997786133	17.049774037590954
8	24.790922441426154	35.61879363368327	21.67509727096101	17.915186653929567
9	26.129706868552333	15.860019705661287	21.158194400632453	36.85207902515393
10-11	23.782964067238986	26.844909019252306	29.662208368577563	19.709918544931142
12-13	24.585567638223793	24.955570280543473	28.807807558124704	21.65105452310803
14-15	22.163910280139433	26.22980028652062	26.36657620321038	25.239713230129567
16-17	21.734689843948832	28.84626431549757	27.174038556841673	22.245007283711924
18-19	21.64501237258419	28.613830500659326	28.371850482168604	21.36930664458788
20-21	22.93018063584237	27.895324793825665	27.606263049886664	21.568231520445302
22-23	22.821715484910975	27.718870154886687	27.245839465915754	22.213574894286577
24-25	22.37164099824135	27.925180187828385	27.682528284076025	22.020650529854247
26-27	22.275481259015333	28.101780167673475	27.2950930117359	22.327645561575295
28-29	22.103082931175756	28.292798237879975	27.37955133856943	22.224567492374845
30-31	22.508178899267467	28.094209859231995	27.348572312062515	22.04903892943802
32-33	22.400126650210968	28.27501010631296	26.96814116279849	22.356722080677585
34-35	22.627064202407748	28.13488189772161	27.317252986512862	21.92080091335778
36-37	22.597151809189484	27.90669640518491	27.269595455015494	22.226556330610116
38-39	22.418306775907098	28.055025800525147	27.486941021890477	22.03972640167728
40-41	22.467250494307358	27.666898476191555	27.662127263623905	22.203723765877186
42-43	22.329010807353644	27.713328965624157	27.407154369955357	22.550505857066845
44-45	22.345850908206334	28.145505438642925	27.348401321969334	22.160242331181404
46-47	22.308770572600018	27.908833833205076	27.183148216208696	22.599247377986206
48-49	22.53298183868577	27.70879570883077	27.40287071275051	22.35535173973295
50-51	22.32950293658557	27.852648341687125	27.249204062020247	22.568644659707054
52-53	22.33565783610113	27.985652538385068	27.205933909433988	22.472755716079813
54-55	22.278332386693435	27.847633049653016	27.227778699745304	22.646255863908245
56-57	22.492366284338086	27.838928015171593	27.146887571087635	22.521818129402682
58-59	22.317916242367485	27.796556885177825	27.403487680844947	22.482039191609747
60-61	22.315235510372787	28.00132608376377	27.200241375293782	22.48319703056966
62-63	22.475392833317915	27.722759742314977	27.137855643798662	22.663991780568445
64-65	22.16012035750417	27.645159618997756	27.282871688912312	22.91184833458576
66-67	22.435256524504204	27.775411162826032	27.014822021790387	22.774510290879377
68-69	22.384288364799783	27.678404293137117	27.20319842610944	22.73410891595366
70-71	22.38880861489507	27.41663787061685	27.189691234923952	23.00486227956413
72-73	22.51625444586851	27.525409136771085	27.283199389167855	22.67513702819255
74-75	22.423829950356865	27.788895723709455	27.124149905665192	22.663124420268485
76-77	22.390862863084358	27.551052209817872	27.162027339262334	22.896057587835433
78-79	22.39786080798987	27.633061097204642	27.125221152492035	22.843856942313458
80-81	22.43892072238576	27.632061828840886	27.254027149462296	22.674990299311055
82-83	22.687003368629863	27.379438151340185	27.575003340580672	22.358555139449276
84-85	22.787201456626956	27.598624322732935	26.96729075449747	22.646883466142636
86-87	22.27807055627395	27.756336354480194	27.181186811508756	22.7844062777371
88-89	22.66284890908181	27.28069691929868	27.188025210288625	22.86842896133089
90-91	22.637790712961944	27.290814731566794	27.344397168369806	22.726997387101456
92-93	22.8220523113751	27.442173014950143	27.23177443215074	22.504000241524018
94-95	22.284081760813955	27.42745068859588	27.412062635996786	22.876404914593387
96-97	22.43949802595571	27.273189420602904	27.322837559625484	22.964474993815895
98-99	22.328458186777368	27.522903437351303	27.163787356067743	22.984851019803582
100	22.55733492884844	27.246653547294404	27.04218922123593	23.153822302621226
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	764.0
1	566.0
2	370.0
3	381.0
4	382.5
5	353.5
6	335.0
7	328.5
8	318.5
9	330.0
10	307.5
11	266.5
12	272.5
13	285.0
14	305.0
15	353.0
16	413.0
17	529.0
18	657.5
19	809.5
20	1182.0
21	1785.5
22	2749.0
23	4116.5
24	5883.5
25	8426.5
26	12220.0
27	16774.5
28	20816.5
29	25707.0
30	32104.0
31	38956.0
32	46715.0
33	53442.0
34	59529.5
35	65062.5
36	71170.0
37	79484.5
38	82585.0
39	81378.5
40	81379.0
41	81243.0
42	81545.0
43	85244.5
44	90206.0
45	95879.0
46	100790.5
47	103993.0
48	107676.0
49	106166.5
50	100870.0
51	99182.0
52	96268.5
53	91205.0
54	85668.5
55	76977.5
56	68749.5
57	59406.0
58	50914.0
59	45292.0
60	36948.0
61	28183.5
62	21997.0
63	16491.5
64	11116.0
65	7080.5
66	4821.5
67	3427.0
68	2448.0
69	1783.0
70	1402.5
71	1094.0
72	946.5
73	882.0
74	711.5
75	488.5
76	332.5
77	256.5
78	209.0
79	141.0
80	98.0
81	85.5
82	73.0
83	57.0
84	44.0
85	28.5
86	21.0
87	20.5
88	18.0
89	14.0
90	10.0
91	7.5
92	6.0
93	7.0
94	6.5
95	5.0
96	4.0
97	3.0
98	3.0
99	4.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05915171226488752
2	0.009930010946376772
3	0.011526600941676566
4	0.009813187288184106
5	0.012344366549025244
6	0.012655896304205692
7	0.002648002919033806
8	0.005685418032043171
9	0.007126243149752743
10-11	0.006892595833367406
12-13	0.005977477177524842
14-15	0.010494658627641335
16-17	0.010027363994870662
18-19	0.007749302660113639
20-21	0.007106772540053965
22-23	0.0024727674317448043
24-25	0.0037772982815629293
26-27	0.004867652424694496
28-29	0.006113771445416287
30-31	0.009248539606919542
32-33	0.010553070456737668
34-35	0.011857601306555794
36-37	0.01882807957871831
38-39	0.01894490323691098
40-41	0.019139609333898758
42-43	0.025194968950218714
44-45	0.028913855402685307
46-47	0.03171762319930934
48-49	0.03273009490364579
50-51	0.03411250819225903
52-53	0.0322822708805739
54-55	0.03890227817815842
56-57	0.041706045974782446
58-59	0.03874651330056819
60-61	0.03956427890791687
62-63	0.03448244977653581
64-65	0.0371499233052684
66-67	0.03954480829821809
68-69	0.042270693656047006
70-71	0.04166710475538489
72-73	0.038279218667797515
74-75	0.04096616280622888
76-77	0.040576750612253326
78-79	0.039817396834000984
80-81	0.04458769621020159
82-83	0.041044045245023995
84-85	0.03685786415978673
86-87	0.03510550928689671
88-89	0.03296374222003113
90-91	0.03506656806749915
92-93	0.03662421684340139
94-95	0.04075198609954232
96-97	0.035358627212980824
98-99	0.03257433002605557
100	0.03025732747190099
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2567973.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.93098150821782
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.3792929271165	42.06451791557564
2	15.077086080126085	17.770149619714367
3	5.817529120701099	10.284981031066653
4	2.95894689435925	6.974945788611339
5	1.58169460899933	4.6605407877293645
6	0.9498746613923816	3.358622766338346
7	0.5846176221382142	2.4116463195723763
8	0.4155393746513528	1.9590514562812213
9	0.28925412505965115	1.534142654555942
>10	0.9316746039143808	8.204697597349424
>50	0.01158202347529749	0.45773831146844834
>100	0.002804123494024292	0.2557557248513183
>500	0.0	0.0
>1k	1.0383457247822393E-4	0.06321002688558638
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.788243879511194E-5
2	0.0	0.0	0.0	1.5576487759022387E-4	7.788243879511194E-5
3	0.0	0.0	0.0	1.9470609698777984E-4	7.788243879511194E-5
4	0.0	0.0	0.0	3.1152975518044775E-4	7.788243879511194E-5
5	0.0	0.0	0.0	3.1152975518044775E-4	1.5576487759022387E-4
6	0.0	0.0	0.0	3.1152975518044775E-4	1.9470609698777984E-4
7	0.0	0.0	0.0	3.504709745780037E-4	1.9470609698777984E-4
8	0.0	0.0	0.0	3.894121939755597E-4	1.9470609698777984E-4
9	0.0	0.0	0.0	4.672946327706717E-4	1.9470609698777984E-4
10-11	0.0	0.0	0.0	7.009419491560074E-4	2.3364731638533584E-4
12-13	0.0	0.0	0.0	7.009419491560074E-4	9.735304849388993E-4
14-15	0.0	0.0	0.0	7.009419491560074E-4	0.001148765972227901
16-17	0.0	0.0	0.0	7.398831685535635E-4	0.0012655896304205692
18-19	0.0	0.0	0.0	7.982949976498973E-4	0.001382413288613237
20-21	0.0	0.0	0.0	8.567068267462314E-4	0.0015576487759022387
22-23	0.0	0.0	0.0	0.001382413288613237	0.0015576487759022387
24-25	0.0	0.0	0.0	0.0020444140183716883	0.0015576487759022387
26-27	0.0	0.0	0.0	0.00290112084511792	0.0016355312146973508
28-29	0.0	0.0	0.0	0.0072430668079454105	0.0017134136534924628
30-31	0.0	0.0	0.0	0.017465136899803856	0.0017718254825887967
32-33	0.0	0.0	0.0	0.03997316171159121	0.0018302373116851306
34-35	0.0	0.0	0.0	0.0765973785549926	0.0019081197504802426
36-37	0.0	0.0	0.0	0.13475608972524244	0.0019081197504802426
38-39	0.0	0.0	0.0	0.21448823644173828	0.002005472798974133
40-41	0.0	0.0	0.0	0.2980560932688934	0.0020638846280704665
42-43	0.0	0.0	0.0	0.3801636543686402	0.0021028258474680224
44-45	0.0	0.0	0.0	0.4793079989548177	0.0022391201153594683
46-47	0.0	0.0	0.0	0.5967352460481476	0.0023559437735521365
48-49	0.0	0.0	0.0	0.721055088974845	0.002453296822046026
50-51	0.0	0.0	0.0	0.8458422265343133	0.002725885357828918
52-53	0.0	0.0	0.0	0.9628995320433665	0.0029400620645154757
54-55	0.0	0.0	0.0	1.0890885535011465	0.0030374151130093657
56-57	0.0	0.0	0.0	1.233579948075778	0.0031931799905995893
58-59	0.0	0.0	0.0	1.3970357164970193	0.0033489448681898134
60-61	0.0	0.0	0.0	1.5657290789272318	0.0033878860875873693
62-63	0.0	0.0	0.0	1.7337993818470832	0.0035825921845751493
64-65	0.0	0.0	0.0	1.9037583339077164	0.0036215334039727052
66-67	0.0	0.0	0.0	2.0910655992099603	0.0036215334039727052
68-69	0.0	0.0	0.0	2.293520998857854	0.0036799452330690393
70-71	0.0	0.0	0.0	2.5134220647958525	0.003835710110659263
72-73	0.0	0.0	0.0	2.7349781325582474	0.004069357427044599
74-75	0.0	0.0	0.0	2.9562810823945576	0.004303004743429935
76-77	0.0	0.0	0.0	3.1923038131631447	0.004400357791923825
78-79	0.0	0.0	0.0	3.4494910966743033	0.0044587696210201585
80-81	0.0	0.0	0.0	3.716530508693043	0.004575593279212827
82-83	0.0	0.0	0.0	3.9925848130023174	0.004867652424694497
84-85	0.0	0.0	0.0	4.265056525127017	0.004906593644092052
86-87	0.0	0.0	0.0	4.557115670608686	0.005159711570176166
88	0.0	0.0	0.0	4.784707627377702	0.005373888276862724
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	3740	0.0	30.29627	1
GTATCAA	5565	0.0	29.73865	1
GCGTAAT	80	4.7731106E-4	29.384869	1
GTACTAG	1030	0.0	27.38784	1
CAACGCA	5950	0.0	26.14227	5
ATCAACG	5970	0.0	25.976482	3
AACGCAG	6525	0.0	24.270191	6
TATCAAC	6785	0.0	23.687378	2
TCAACGC	6665	0.0	23.33872	4
GTACATG	8830	0.0	23.268291	1
ACATGGG	8090	0.0	23.119331	3
TACATGG	8695	0.0	22.699717	2
GCATCGT	875	0.0	22.555275	8
TATTCGC	210	3.868263E-8	22.376265	9
TCTAACG	405	0.0	22.046484	2
CGCAGAG	7075	0.0	21.652	8
ACGCAGA	7200	0.0	21.471054	7
TAACGCC	455	0.0	20.657024	4
CGCGGTA	115	0.003956712	20.437666	94
CTAACGC	445	0.0	20.064775	3
>>END_MODULE