FastQCFastQC Report
Mon 16 Jan 2017
ERR1146488_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146488_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2567973
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88510.3446687328877679No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75600.2943956186455231No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43430.1691217158435856No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63500.050.6079181
GTATCAA108050.039.8444481
CAACGCA131850.032.1976135
ATCAACG132050.032.1132473
TATCAAC137300.031.150492
TCAACGC138750.030.7658524
AACGCAG139900.030.6384186
CGCAGAG153150.027.5569848
ACGCAGA154450.027.416327
GTATCGA1205.848653E-627.4155889
GCAGAGT168050.024.750599
GTACATG89400.023.5256421
TACATGG87550.022.8404312
GAGTACT113500.022.67091412-13
ACATGGG84400.021.9467333
CAGAGTA163050.021.7912310-11
AGAGTAC155950.021.36690510-11
GTACTAG11500.021.2753661
CATGGGG55050.021.0938424
GTACTTT121900.020.93518814-15
Produced by FastQC (version 0.11.5)