##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146488_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2567973
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.5326360518588	34.0	31.0	34.0	31.0	34.0
2	32.64069404156508	34.0	31.0	34.0	31.0	34.0
3	32.75666449764075	34.0	31.0	34.0	31.0	34.0
4	36.20826309310885	37.0	37.0	37.0	35.0	37.0
5	36.239900107984	37.0	37.0	37.0	35.0	37.0
6	36.32560583775608	37.0	37.0	37.0	35.0	37.0
7	36.306685856899584	37.0	37.0	37.0	35.0	37.0
8	36.31780591151075	37.0	37.0	37.0	35.0	37.0
9	38.136434066869086	39.0	39.0	39.0	37.0	39.0
10-11	38.10730447711093	39.0	38.5	39.0	37.0	39.0
12-13	38.09227978643078	39.0	38.5	39.0	37.0	39.0
14-15	39.55344507126827	41.0	39.0	41.0	37.0	41.0
16-17	39.55019523180345	41.0	39.0	41.0	37.0	41.0
18-19	39.60925114866862	41.0	39.5	41.0	37.0	41.0
20-21	39.64292147931462	41.0	40.0	41.0	37.0	41.0
22-23	39.60268565907819	41.0	40.0	41.0	37.0	41.0
24-25	39.59005565868489	41.0	40.0	41.0	37.0	41.0
26-27	39.52227048337346	41.0	39.0	41.0	36.5	41.0
28-29	39.434902158239204	41.0	39.0	41.0	36.0	41.0
30-31	39.328097686385334	41.0	39.0	41.0	36.0	41.0
32-33	39.20191703728972	41.0	39.0	41.0	35.0	41.0
34-35	39.118548948918075	41.0	39.0	41.0	35.0	41.0
36-37	39.02874192991905	41.0	39.0	41.0	35.0	41.0
38-39	38.9306684688663	41.0	39.0	41.0	35.0	41.0
40-41	38.84326918546262	41.0	39.0	41.0	35.0	41.0
42-43	38.75020706993415	41.0	39.0	41.0	35.0	41.0
44-45	38.59927966532358	40.5	38.0	41.0	35.0	41.0
46-47	38.36018564058111	40.0	38.0	41.0	34.5	41.0
48-49	38.14421919545104	40.0	38.0	41.0	34.0	41.0
50-51	37.99974941325318	40.0	38.0	41.0	34.0	41.0
52-53	37.88020181676365	40.0	37.5	41.0	33.0	41.0
54-55	37.754472106988665	40.0	37.0	41.0	33.0	41.0
56-57	37.57414213467197	40.0	37.0	41.0	33.0	41.0
58-59	37.38540280602639	40.0	36.0	41.0	33.0	41.0
60-61	37.22159812427934	39.5	36.0	41.0	33.0	41.0
62-63	36.97824821366892	39.0	35.0	41.0	32.5	41.0
64-65	36.69703049837362	39.0	35.0	41.0	32.0	41.0
66-67	36.45182854336864	38.5	35.0	41.0	32.0	41.0
68-69	36.1424378293697	37.0	35.0	40.0	31.5	41.0
70-71	35.86197167960878	37.0	35.0	40.0	31.0	41.0
72-73	35.53129744744201	36.5	35.0	39.0	31.0	41.0
74-75	35.20097018154007	36.0	35.0	39.0	31.0	41.0
76-77	34.8762749841996	35.5	35.0	38.5	31.0	40.0
78-79	34.57042986822681	35.0	35.0	37.0	31.0	39.0
80-81	34.295044184654586	35.0	35.0	37.0	31.0	39.0
82-83	34.02427985029438	35.0	35.0	36.0	30.0	38.5
84-85	33.797282915357755	35.0	34.5	36.0	30.0	37.0
86-87	33.595966351671144	35.0	34.0	36.0	30.0	37.0
88-89	33.4344237653589	35.0	34.0	35.0	30.0	36.5
90-91	33.33709797571859	35.0	34.0	35.0	30.0	36.0
92-93	33.248220873038775	35.0	34.0	35.0	30.0	36.0
94-95	33.16839156797988	35.0	34.0	35.0	30.0	36.0
96-97	33.11582754180048	35.0	34.0	35.0	30.0	36.0
98-99	33.06304700244122	35.0	34.0	35.0	30.0	35.0
100	32.52870415693623	35.0	33.0	35.0	27.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3210757501712891
1101	2	0.3010431582644344
1101	3	0.32703945163120807
1101	4	0.2259330155698578
1101	5	0.22715513545768573
1101	6	0.16446913994253975
1101	7	0.2024788274135929
1101	8	0.1962799942540272
1101	9	0.18000746062055129
1101	10-11	0.19645540748957302
1101	12-13	0.20081451701665998
1101	14-15	0.3188536365575416
1101	16-17	0.35018615551820886
1101	18-19	0.34579880912053795
1101	20-21	0.328397514929442
1101	22-23	0.32790081129012805
1101	24-25	0.31211841186735256
1101	26-27	0.3201094339115258
1101	28-29	0.38089516532107837
1101	30-31	0.37674360647069705
1101	32-33	0.3857110533062098
1101	34-35	0.40306830191391896
1101	36-37	0.45414220334616573
1101	38-39	0.4655603442458798
1101	40-41	0.4511923885789244
1101	42-43	0.4116081469114832
1101	44-45	0.4079766882482829
1101	46-47	0.432768173645691
1101	48-49	0.3474246880024623
1101	50-51	0.27150863605272946
1101	52-53	0.3856811061550971
1101	54-55	0.3806753513509591
1101	56-57	0.4203861416936405
1101	58-59	0.40147443059025534
1101	60-61	0.4031087418755348
1101	62-63	0.42925902088955326
1101	64-65	0.40784855816393417
1101	66-67	0.32293847906505846
1101	68-69	0.3350625671942069
1101	70-71	0.314976292930524
1101	72-73	0.3307033443937897
1101	74-75	0.33603756050782607
1101	76-77	0.28537031921676004
1101	78-79	0.20335733277371304
1101	80-81	0.2870961512061143
1101	82-83	0.25443694091292457
1101	84-85	0.24807494789476436
1101	86-87	0.273496572541859
1101	88-89	0.1782139135261005
1101	90-91	0.2375829863829395
1101	92-93	0.3032832967842083
1101	94-95	0.3135716339536643
1101	96-97	0.28133814047887995
1101	98-99	0.2595642661857056
1101	100	0.2544746412399377
1102	1	0.23391837772553714
1102	2	0.26863448097697784
1102	3	0.21974670512570071
1102	4	0.2042158113988748
1102	5	0.12493735632253333
1102	6	0.12258974004452483
1102	7	0.1810575529370979
1102	8	0.1742115964094566
1102	9	0.2611806841044384
1102	10-11	0.21098059631693644
1102	12-13	0.20464717930860843
1102	14-15	0.3272170942966852
1102	16-17	0.3098830350238586
1102	18-19	0.31334365985065915
1102	20-21	0.2617235499601591
1102	22-23	0.2753451318182911
1102	24-25	0.2668587526030066
1102	26-27	0.2958886580425286
1102	28-29	0.31830121351791263
1102	30-31	0.36436767592698516
1102	32-33	0.3297332613225805
1102	34-35	0.3561275075436541
1102	36-37	0.43197766076377064
1102	38-39	0.46765733674286736
1102	40-41	0.47190621119402465
1102	42-43	0.4335445027414977
1102	44-45	0.4810061728776418
1102	46-47	0.544000950144472
1102	48-49	0.5412271568761611
1102	50-51	0.5781914286728593
1102	52-53	0.5397418458059207
1102	54-55	0.52271759361318
1102	56-57	0.536310562611007
1102	58-59	0.5071986362698198
1102	60-61	0.46896420031576014
1102	62-63	0.4330985709208406
1102	64-65	0.4995513928900337
1102	66-67	0.4527518237601882
1102	68-69	0.48038864047213536
1102	70-71	0.42997629819762295
1102	72-73	0.427370010790348
1102	74-75	0.4053987972346462
1102	76-77	0.4015905887963882
1102	78-79	0.3888604404001512
1102	80-81	0.39826110373055457
1102	82-83	0.339574463542192
1102	84-85	0.44101527438495225
1102	86-87	0.4056919405938757
1102	88-89	0.38402624985619127
1102	90-91	0.3997166832376493
1102	92-93	0.3769641994897057
1102	94-95	0.35563955532874303
1102	96-97	0.3980226842054506
1102	98-99	0.42114131215423356
1102	100	0.3928463261811288
1103	1	0.2428232548522189
1103	2	0.2336102065257748
1103	3	0.21327785630676743
1103	4	0.14778211372587435
1103	5	0.14341013184982643
1103	6	0.1602525261790433
1103	7	0.14073389891060373
1103	8	0.17222979835548102
1103	9	0.20252382116755996
1103	10-11	0.19420230164055852
1103	12-13	0.1812949217108013
1103	14-15	0.2414946873474122
1103	16-17	0.26571275576686304
1103	18-19	0.23256516875901667
1103	20-21	0.24534339540689842
1103	22-23	0.226452861996421
1103	24-25	0.2372317859053794
1103	26-27	0.23888415845631528
1103	28-29	0.2784303525603562
1103	30-31	0.3046726864723297
1103	32-33	0.24136679355963508
1103	34-35	0.22153251222049164
1103	36-37	0.18840185704809187
1103	38-39	0.21542812332609884
1103	40-41	0.18650593325845222
1103	42-43	0.21643226219827483
1103	44-45	0.18651466284749318
1103	46-47	0.15789681093235686
1103	48-49	0.13204115042559295
1103	50-51	0.1530074789934872
1103	52-53	0.0668618032333157
1103	54-55	0.018005623420393135
1103	56-57	0.10241132443874079
1103	58-59	0.07010086338733856
1103	60-61	0.08104034556244955
1103	62-63	0.06292183311484933
1103	64-65	0.05392245086167691
1103	66-67	0.019855181702645552
1103	68-69	0.10316358890315058
1103	70-71	0.12593074263186566
1103	72-73	0.11388538447637586
1103	74-75	0.037016185095311016
1103	76-77	-0.013067459019545424
1103	78-79	-0.08651651122700343
1103	80-81	-0.04535250593084328
1103	82-83	-0.0866693700365957
1103	84-85	-0.02654969934451401
1103	86-87	-0.07476573890352256
1103	88-89	-0.08680005557672388
1103	90-91	-0.08041028405880724
1103	92-93	-0.04819511843833624
1103	94-95	-0.033490776177899306
1103	96-97	-0.028613780287898294
1103	98-99	-0.08356486937738339
1103	100	-0.03544713259182686
1104	1	0.18211206243954337
1104	2	0.2256738532167475
1104	3	0.1966519057020122
1104	4	0.16840997624823473
1104	5	0.1854803550218591
1104	6	0.13281863118516668
1104	7	0.10603062329236934
1104	8	0.09563081321832811
1104	9	0.17911374705990113
1104	10-11	0.1450471046474675
1104	12-13	0.16331990172549382
1104	14-15	0.2542404004104313
1104	16-17	0.2766096247933021
1104	18-19	0.23474397399245106
1104	20-21	0.21606562190394385
1104	22-23	0.2868845444877053
1104	24-25	0.2307307353741308
1104	26-27	0.2428290236649957
1104	28-29	0.2766830554561679
1104	30-31	0.28964935038836614
1104	32-33	0.28300039145576505
1104	34-35	0.2548961946556787
1104	36-37	0.2831930566171863
1104	38-39	0.26402297380876405
1104	40-41	0.2945374654951536
1104	42-43	0.36105897393120756
1104	44-45	0.2975695569434009
1104	46-47	0.36163589103617255
1104	48-49	0.42535091708749917
1104	50-51	0.3927552756828092
1104	52-53	0.339400692600222
1104	54-55	0.38484382189669475
1104	56-57	0.41290094440152814
1104	58-59	0.49980408025315626
1104	60-61	0.4657004665772817
1104	62-63	0.437622697988445
1104	64-65	0.3910710402917772
1104	66-67	0.43267890282346144
1104	68-69	0.38428078710236946
1104	70-71	0.3558877699714529
1104	72-73	0.3706880244691888
1104	74-75	0.3932543090507963
1104	76-77	0.34763998081891856
1104	78-79	0.37233882476295577
1104	80-81	0.3788056192072702
1104	82-83	0.2973710834714751
1104	84-85	0.3312922520850492
1104	86-87	0.3386439902705476
1104	88-89	0.3367517824454964
1104	90-91	0.2631176815266798
1104	92-93	0.2818356716225665
1104	94-95	0.3323079971472893
1104	96-97	0.30539216090888033
1104	98-99	0.3187834839816901
1104	100	0.344730709101853
1105	1	0.13009188473505162
1105	2	0.10779786148632553
1105	3	0.11532558424876527
1105	4	0.10109914187228952
1105	5	0.09277614423137237
1105	6	0.08277730960284657
1105	7	0.13318225596956523
1105	8	0.0875425783948316
1105	9	0.13065915148195728
1105	10-11	0.057085221913716566
1105	12-13	0.12021559945220162
1105	14-15	0.17210684845898072
1105	16-17	0.14701862134199928
1105	18-19	0.15764773662814235
1105	20-21	0.18818907476252633
1105	22-23	0.2060481012202402
1105	24-25	0.16185326508416864
1105	26-27	0.11259691520609749
1105	28-29	0.22091694706170273
1105	30-31	0.24550445254950404
1105	32-33	0.1582428859580176
1105	34-35	0.21009554204420056
1105	36-37	0.17476342537666056
1105	38-39	0.1634425942106006
1105	40-41	0.22541198037279742
1105	42-43	0.20541819925448124
1105	44-45	0.16685315285480584
1105	46-47	0.15397632540454964
1105	48-49	0.05417501245206324
1105	50-51	0.1204828859171414
1105	52-53	0.04771355952438938
1105	54-55	0.12514398847021369
1105	56-57	0.16735253507087577
1105	58-59	0.15889540795495805
1105	60-61	0.1532334952192116
1105	62-63	0.11087867724381084
1105	64-65	0.1185063308218659
1105	66-67	0.08164009580929843
1105	68-69	0.14481373701958233
1105	70-71	0.08365435142532363
1105	72-73	0.12108223471138047
1105	74-75	0.09565140885246137
1105	76-77	0.09304679945959293
1105	78-79	0.12329242845935795
1105	80-81	0.10797000870071827
1105	82-83	0.10844056680910796
1105	84-85	0.052455985325913446
1105	86-87	0.039176800801648426
1105	88-89	0.040383836102890314
1105	90-91	0.09420542871212234
1105	92-93	0.03860999675732302
1105	94-95	0.03622330329999812
1105	96-97	0.06242107904720484
1105	98-99	-2.0295282494942057E-4
1105	100	-0.036012203238719565
1106	1	0.15673199773134172
1106	2	0.1533519316553722
1106	3	0.19371930484412303
1106	4	0.13211160651798792
1106	5	0.1326658972409973
1106	6	0.0841724426518553
1106	7	0.0888213826539399
1106	8	0.09364719619036777
1106	9	0.10781424301651299
1106	10-11	0.1486134898881275
1106	12-13	0.1331006477196297
1106	14-15	0.19161448292560124
1106	16-17	0.16596498590625686
1106	18-19	0.17916528232669293
1106	20-21	0.1911078707059346
1106	22-23	0.2043295128490925
1106	24-25	0.18527329305830875
1106	26-27	0.1786665481399794
1106	28-29	0.2753687878908906
1106	30-31	0.20172395678171284
1106	32-33	0.1963427062224241
1106	34-35	0.20802913266633283
1106	36-37	0.21772521352235685
1106	38-39	0.2565888948535857
1106	40-41	0.17278622560362322
1106	42-43	0.13981859377924621
1106	44-45	0.0771370869678023
1106	46-47	0.10519536569212562
1106	48-49	0.10415112789523562
1106	50-51	0.19592213838205907
1106	52-53	0.191537494255968
1106	54-55	0.1050953601998259
1106	56-57	0.16755996986947252
1106	58-59	0.1869674946404274
1106	60-61	0.15842847224536172
1106	62-63	0.12040026441557217
1106	64-65	0.20818325352868783
1106	66-67	0.25490344796389053
1106	68-69	0.2750464310257641
1106	70-71	0.13128032957524738
1106	72-73	0.08716327724651052
1106	74-75	0.07438178141718055
1106	76-77	0.07770095451332537
1106	78-79	0.1142180151774781
1106	80-81	0.12190941409553346
1106	82-83	0.08742291285680892
1106	84-85	0.07017863579002892
1106	86-87	0.06819141677578244
1106	88-89	0.0916209874141316
1106	90-91	0.09385344938687723
1106	92-93	0.05303214513025267
1106	94-95	0.09866884135548304
1106	96-97	0.024838127465841353
1106	98-99	-0.011935356436161726
1106	100	0.0067381292549271166
1107	1	0.12851413451348037
1107	2	0.11635338965682962
1107	3	0.13912903025384793
1107	4	0.11046365987004236
1107	5	0.11883216340726221
1107	6	0.06256054103995012
1107	7	0.08851128734384162
1107	8	0.0946459271890987
1107	9	0.10690893211120311
1107	10-11	0.11354031981496604
1107	12-13	0.13010884222781982
1107	14-15	0.18707787088899153
1107	16-17	0.15647332891460053
1107	18-19	0.15152103468244604
1107	20-21	0.1250224696205322
1107	22-23	0.14499006850964946
1107	24-25	0.1311130613980822
1107	26-27	0.22101210798553694
1107	28-29	0.20519497521707564
1107	30-31	0.20279234785009947
1107	32-33	0.10742891730863846
1107	34-35	0.10166920880640617
1107	36-37	0.07520118349832927
1107	38-39	0.07530943107412469
1107	40-41	0.06314721596461226
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2216	48-49	-0.49022219474412054
2216	50-51	-0.5255345047372941
2216	52-53	-0.5516892077830846
2216	54-55	-0.5774573127803961
2216	56-57	-0.4923141702421958
2216	58-59	-0.3551554655347786
2216	60-61	-0.3899520071327416
2216	62-63	-0.439780504933843
2216	64-65	-0.3707888907166179
2216	66-67	-0.40772140936405066
2216	68-69	-0.45100779977905603
2216	70-71	-0.47144817707250297
2216	72-73	-0.40602980045962767
2216	74-75	-0.3946653234969091
2216	76-77	-0.4403105826431073
2216	78-79	-0.4649688312302729
2216	80-81	-0.4044462017850208
2216	82-83	-0.4018197684523699
2216	84-85	-0.4224335738530556
2216	86-87	-0.34994536330874126
2216	88-89	-0.3465398541196265
2216	90-91	-0.28287542743701266
2216	92-93	-0.30002026564187645
2216	94-95	-0.30053250361783057
2216	96-97	-0.3079810723367302
2216	98-99	-0.3207680676679203
2216	100	-0.4331045458846603
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	15.0
8	56.0
9	112.0
10	192.0
11	176.0
12	140.0
13	165.0
14	237.0
15	465.0
16	929.0
17	1839.0
18	3388.0
19	5476.0
20	7845.0
21	11323.0
22	16346.0
23	19860.0
24	18951.0
25	15323.0
26	13083.0
27	13454.0
28	15848.0
29	18940.0
30	23129.0
31	29041.0
32	37882.0
33	50450.0
34	74817.0
35	118702.0
36	215365.0
37	560458.0
38	909741.0
39	382993.0
40	1232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.3576524339546	20.191547917939634	13.721032300977834	23.729767347127932
2	14.947955764153436	21.443994665007864	40.3610395907241	23.247009980114605
3	17.597775044091403	27.16065391958834	31.222436789343767	24.019134246976492
4	11.884120276965529	18.32702290872996	39.95193874701954	29.836918067284973
5	12.212860230954512	38.61341589926603	35.76884915031132	13.40487471946814
6	28.42703267063654	39.38499307394812	18.639783382226547	13.548190873188801
7	24.733301115939394	33.97390391090865	23.840222494724433	17.452572478427523
8	25.06654080864557	34.81528037872672	21.557313881415418	18.560864931212283
9	26.354404816561544	14.835319530228706	21.246835539158706	37.56344011405105
10-11	23.7962392906779	26.3933460359591	29.549142455937037	20.26127221742596
12-13	25.105189968897644	24.644904755618533	29.123086574508378	21.126818700975438
14-15	22.326392188076817	25.50750163942597	27.56122340897798	24.604882763519228
16-17	20.953783392582398	28.27078010555407	28.238809364428676	22.536627137434856
18-19	20.871481125385664	27.065997189222784	30.93346386430075	21.1290578210908
20-21	22.076439277204237	26.356624466067206	30.561516807224997	21.005419449503556
22-23	21.87785063060695	26.308444924721446	30.246138718925998	21.567565725745606
24-25	21.508832063265462	26.461824170269704	30.667748453741535	21.361595312723303
26-27	21.459143067314184	26.71416327196587	30.273196018805493	21.55349764191446
28-29	21.24193721738399	26.86534354735955	30.36423294334985	21.52848629190661
30-31	21.642523082434764	26.602374638333004	30.315112715491228	21.439989563741012
32-33	21.62109117162921	26.776649894448347	29.938838063394858	21.663420870527585
34-35	21.801089976615543	26.73739118244355	30.153683198043574	21.307835642897334
36-37	21.815370242981235	26.545047217523827	30.021548270598924	21.618034268896015
38-39	21.724327602773798	26.698124871614894	30.127698764485007	21.449848761126304
40-41	21.777341544318368	26.414050628869347	30.15348477496766	21.65512305184463
42-43	21.84063296960738	26.44798846087496	29.76363719202682	21.94774137749084
44-45	21.869789765232163	26.966047985513807	29.49324610464522	21.67091614460881
46-47	22.022866355265748	26.85889410486514	28.866320218538803	22.251919321330313
48-49	22.327887818884644	26.852851170065513	28.743441521257484	22.075819489792362
50-51	22.099692888099934	27.19650819630317	28.41331229715145	22.290486618445446
52-53	22.0539638356318	27.349858369075314	28.33044905219822	22.265728743094666
54-55	22.06851057043984	27.241665934886704	28.21552065381603	22.474302840857426
56-57	22.330730548380043	27.16644706905827	28.079294239831743	22.423528142729943
58-59	22.011764016378265	27.198480635363588	28.469277902641267	22.32047744561688
60-61	22.090453772016026	27.427260979220424	28.193520034907486	22.28876521385606
62-63	22.183278490624815	27.170544597830954	28.171479195467203	22.474697716077028
64-65	21.838219688340313	27.109524838727893	28.279893997542704	22.77236147538909
66-67	22.055622775149356	27.220664294011883	28.087803544495454	22.635909386343307
68-69	22.013425432383624	27.029357789233366	28.332043362491543	22.625173415891467
70-71	22.037620875286613	26.823682521682784	28.208510648240452	22.93018595479015
72-73	22.175957729519393	26.905742716457855	28.236645216765506	22.68165433725725
74-75	22.113533723615	27.177712899584055	27.926109734493977	22.78264364230697
76-77	22.062168575043547	27.00872195020891	27.88644680513838	23.04266266960916
78-79	22.030880998865047	27.053399894741126	27.950826231539537	22.964892874854286
80-81	21.992957867141854	27.09513577250842	28.01769256051078	22.89421379983894
82-83	22.270236801831796	26.828175562798627	28.287947289103844	22.61364034626573
84-85	22.382714287411012	26.992035149936495	27.675765745751363	22.949484816901126
86-87	21.8272976054066	27.163005055544794	27.898974774846693	23.110722564201915
88-89	22.150684899500305	26.745290439756257	27.89380553028698	23.210219130456455
90-91	22.08584788193799	26.74662598606488	28.073373059875056	23.094153072122072
92-93	22.18298245347595	26.91371755076864	27.88031649865478	23.022983497100633
94-95	21.71498687875612	26.91251037296732	28.082129368182606	23.29037338009395
96-97	21.897345054171364	26.656761276062497	28.041419616022356	23.404474053743787
98-99	21.713387613784406	26.903033322760738	27.872577429667405	23.511001633787448
100	21.910386919376577	26.609386238288813	27.791924378619377	23.688302463715235
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	235.0
1	160.0
2	219.5
3	342.5
4	228.5
5	178.0
6	369.0
7	929.5
8	1818.0
9	2244.5
10	1686.5
11	1157.5
12	1177.5
13	1345.5
14	1563.5
15	1829.0
16	2189.0
17	2608.5
18	3024.5
19	3454.0
20	4063.5
21	4805.5
22	5807.5
23	7259.0
24	9113.0
25	11417.0
26	14869.0
27	19254.5
28	23244.5
29	28103.0
30	33996.0
31	40179.0
32	47656.5
33	53612.0
34	58548.5
35	63857.5
36	69614.0
37	77360.0
38	80024.5
39	79109.5
40	79763.0
41	79134.5
42	79668.5
43	83398.0
44	87454.0
45	93453.0
46	98738.0
47	101877.0
48	106050.0
49	104322.5
50	99466.0
51	97638.0
52	93736.5
53	88947.5
54	84274.0
55	75923.5
56	67398.0
57	57930.5
58	49702.0
59	44816.5
60	36882.0
61	27805.0
62	21202.0
63	15742.5
64	10487.0
65	6608.5
66	4469.5
67	3058.5
68	2095.5
69	1523.5
70	1206.0
71	954.5
72	847.0
73	796.5
74	593.5
75	401.5
76	313.0
77	232.5
78	153.0
79	97.0
80	76.0
81	61.5
82	42.0
83	31.5
84	23.5
85	14.0
86	8.5
87	8.5
88	3.5
89	2.0
90	2.5
91	1.0
92	1.5
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006074830226018731
2	0.0884355092518496
3	4.672946327706717E-4
4	0.0
5	0.037928747693219515
6	0.005140240960477388
7	7.788243879511194E-5
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.894121939755597E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.0888280367433764E-4
24-25	0.0
26-27	0.0
28-29	3.894121939755597E-5
30-31	1.1682365819266792E-4
32-33	0.001401883898312015
34-35	0.0010903541431315671
36-37	6.814713394572295E-4
38-39	6.035889006621175E-4
40-41	0.001207177801324235
42-43	5.451770715657836E-4
44-45	4.0888280367433764E-4
46-47	3.1152975518044775E-4
48-49	7.204125588547854E-4
50-51	6.425301200596735E-4
52-53	0.001207177801324235
54-55	0.001304530849818125
56-57	7.204125588547854E-4
58-59	0.0022391201153594683
60-61	0.001655001824396129
62-63	8.956480461437872E-4
64-65	0.004205651694936045
66-67	0.004049886817345821
68-69	0.0033294742584910357
70-71	0.0014408251177095708
72-73	0.00502341730228472
74-75	0.003271062429394701
76-77	0.003271062429394701
78-79	7.204125588547854E-4
80-81	3.894121939755597E-5
82-83	1.1682365819266792E-4
84-85	9.735304849388994E-5
86-87	1.9470609698777984E-5
88-89	1.9470609698777987E-4
90-91	1.9470609698777984E-5
92-93	0.0
94-95	0.0
96-97	4.283534133731157E-4
98-99	5.257064618670056E-4
100	8.567068267462314E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2567973.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.79551109561823
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.69094106648592	37.63955223377087
2	16.309867979319424	17.87415103657732
3	6.475318806039404	10.644552104519926
4	3.1795676190216304	6.9690413098947275
5	1.7744236957591248	4.861522665464851
6	1.0048708198452772	3.303744609509693
7	0.6709819119885918	2.5736757762331015
8	0.4575975387036189	2.0059432807489395
9	0.3369732076738462	1.6618157226016474
>10	1.0686552345763012	8.87818271753518
>50	0.01952460778119857	0.7440333115690827
>100	0.009267869166523546	0.9289303452482334
>500	0.0015630561638484816	0.5630162785578999
>1k	2.2329373769264024E-4	0.34785510810390297
>5k	2.2329373769264024E-4	1.0039834996646415
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8851	0.3446687328877679	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7560	0.2943956186455231	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4343	0.1691217158435856	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.788243879511194E-5
2	0.0	0.0	0.0	2.725885357828918E-4	7.788243879511194E-5
3	0.0	0.0	0.0	2.725885357828918E-4	7.788243879511194E-5
4	0.0	0.0	0.0	3.1152975518044775E-4	7.788243879511194E-5
5	0.0	0.0	0.0	3.1152975518044775E-4	1.1682365819266792E-4
6	0.0	0.0	0.0	3.1152975518044775E-4	1.5576487759022387E-4
7	0.0	0.0	0.0	3.1152975518044775E-4	1.5576487759022387E-4
8	0.0	0.0	0.0	3.1152975518044775E-4	1.5576487759022387E-4
9	0.0	0.0	0.0	3.1152975518044775E-4	1.5576487759022387E-4
10-11	0.0	0.0	0.0	5.841182909633396E-4	1.9470609698777984E-4
12-13	0.0	0.0	0.0	5.841182909633396E-4	4.672946327706717E-4
14-15	0.0	0.0	0.0	6.425301200596735E-4	7.593537782523414E-4
16-17	0.0	0.0	0.0	6.814713394572295E-4	0.0010708835334327892
18-19	0.0	0.0	0.0	7.204125588547855E-4	0.001207177801324235
20-21	0.0	0.0	0.0	7.593537782523414E-4	0.0015965899952997947
22-23	0.0	0.0	0.0	0.001207177801324235	0.0017134136534924628
24-25	0.0	0.0	0.0	0.0018691785310826865	0.0018691785310826867
26-27	0.0	0.0	0.0	0.002725885357828918	0.0020638846280704665
28-29	0.0	0.0	0.0	0.0070483607109576305	0.002200178895961912
30-31	0.0	0.0	0.0	0.017387254461008743	0.0022391201153594683
32-33	0.0	0.0	0.0	0.040265220857072875	0.0023364731638533583
34-35	0.0	0.0	0.0	0.07716202623625715	0.002453296822046026
36-37	0.0	0.0	0.0	0.13547650228409722	0.002453296822046026
38-39	0.0	0.0	0.0	0.21462453070962972	0.002492238041443582
40-41	0.0	0.0	0.0	0.29825079936588117	0.0025311792608411383
42-43	0.0	0.0	0.0	0.38057253717231454	0.002589591089937472
44-45	0.0	0.0	0.0	0.48057358858523824	0.002686944138431362
46-47	0.0	0.0	0.0	0.5986044245792304	0.002823238406322808
48-49	0.0	0.0	0.0	0.7239172686005655	0.003173709380900812
50-51	0.0	0.0	0.0	0.8495416423770811	0.0033100036487922575
52-53	0.0	0.0	0.0	0.9670857131286037	0.0034268273069849257
54-55	0.0	0.0	0.0	1.0937614998288534	0.003660474623370261
56-57	0.0	0.0	0.0	1.2388564833041469	0.003757827671864151
58-59	0.0	0.0	0.0	1.4032663116006283	0.0038162395009604853
60-61	0.0	0.0	0.0	1.5733031461000564	0.0038746513300568193
62-63	0.0	0.0	0.0	1.7418407436526786	0.004166710475538489
64-65	0.0	0.0	0.0	1.9123838140042748	0.004244592914333601
66-67	0.0	0.0	0.0	2.1000026090616997	0.004341945962827491
68-69	0.0	0.0	0.0	2.3031784212684476	0.004400357791923825
70-71	0.0	0.0	0.0	2.523955664642892	0.004497710840417715
72-73	0.0	0.0	0.0	2.7459984976477556	0.00492606425379083
74-75	0.0	0.0	0.0	2.9683333897981017	0.005257064618670056
76-77	0.0	0.0	0.0	3.2055438277583135	0.005276535228368834
78-79	0.0	0.0	0.0	3.4638798772417	0.00541282949626028
80-81	0.0	0.0	0.0	3.7322627613296557	0.005451770715657837
82-83	0.0	0.0	0.0	4.009543714049952	0.005646476812645616
84-85	0.0	0.0	0.0	4.283378368853567	0.005646476812645616
86-87	0.0	0.0	0.0	4.577228810427524	0.005841182909633396
88	0.0	0.0	0.0	4.806826239995514	0.006035889006621176
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	6350	0.0	50.607918	1
GTATCAA	10805	0.0	39.844448	1
CAACGCA	13185	0.0	32.197613	5
ATCAACG	13205	0.0	32.113247	3
TATCAAC	13730	0.0	31.15049	2
TCAACGC	13875	0.0	30.765852	4
AACGCAG	13990	0.0	30.638418	6
CGCAGAG	15315	0.0	27.556984	8
ACGCAGA	15445	0.0	27.41632	7
GTATCGA	120	5.848653E-6	27.415588	9
GCAGAGT	16805	0.0	24.75059	9
GTACATG	8940	0.0	23.525642	1
TACATGG	8755	0.0	22.840431	2
GAGTACT	11350	0.0	22.670914	12-13
ACATGGG	8440	0.0	21.946733	3
CAGAGTA	16305	0.0	21.79123	10-11
AGAGTAC	15595	0.0	21.366905	10-11
GTACTAG	1150	0.0	21.275366	1
CATGGGG	5505	0.0	21.093842	4
GTACTTT	12190	0.0	20.935188	14-15
>>END_MODULE