FastQCFastQC Report
Mon 16 Jan 2017
ERR1146487_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146487_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2965078
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG119300.028.85041
TAGTCCG1154.205942E-628.6029155
TACATGG121000.027.652012
ACATGGG118750.026.9887563
GTACTAG13350.026.0634371
GTGATCG6900.025.1969578
CATGGGG80300.024.4620484
CTAACGC4650.024.2543893
GTATAAG14950.022.645011
TGATCGC7750.022.433429
ATGGGGG54500.022.2450455
GTATTAG6700.021.0536631
CATGGGA38100.019.734544
GCATCGT14400.019.5787168
TAGGCAT18800.019.4960615
TCTAACG6100.019.2593772
GCTATTG17650.019.1682838
TAACGCC6900.018.388524
ATTTAGG20400.018.2086661
TACTAGG18450.018.083982
Produced by FastQC (version 0.11.5)