##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146487_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2965078
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.26808569622789	31.0	31.0	34.0	28.0	34.0
2	31.31104544298666	31.0	31.0	34.0	28.0	34.0
3	31.512797639724823	33.0	31.0	34.0	28.0	34.0
4	35.01341448690388	37.0	35.0	37.0	32.0	37.0
5	35.09103773998525	37.0	35.0	37.0	32.0	37.0
6	35.02095897645863	37.0	35.0	37.0	32.0	37.0
7	35.00714922170682	37.0	35.0	37.0	32.0	37.0
8	35.01800694619163	37.0	35.0	37.0	32.0	37.0
9	36.606854524569	39.0	37.0	39.0	32.0	39.0
10-11	36.57796810067054	39.0	37.0	39.0	32.0	39.0
12-13	36.52297797899415	39.0	37.0	39.0	32.0	39.0
14-15	37.890966274748926	40.0	38.0	41.0	32.0	41.0
16-17	37.9108409626998	40.0	38.0	41.0	32.0	41.0
18-19	37.93292554192504	40.0	38.0	41.0	32.0	41.0
20-21	38.03626565641781	40.0	38.0	41.0	32.0	41.0
22-23	38.05844601727172	40.0	38.0	41.0	32.0	41.0
24-25	38.11357036813197	40.0	38.0	41.0	32.0	41.0
26-27	38.152100045934716	40.0	38.0	41.0	32.5	41.0
28-29	38.1184643371945	40.0	38.0	41.0	32.0	41.0
30-31	38.10421445911372	40.0	38.0	41.0	32.0	41.0
32-33	38.117453233945284	40.0	38.0	41.0	33.0	41.0
34-35	38.05178700189337	40.0	38.0	41.0	32.0	41.0
36-37	37.9511709304106	40.0	38.0	41.0	32.0	41.0
38-39	37.901203273573245	40.0	38.0	41.0	32.0	41.0
40-41	37.837914213386625	40.0	38.0	41.0	32.0	41.0
42-43	37.69517614713677	40.0	37.5	41.0	31.5	41.0
44-45	37.51877134429516	40.0	37.0	41.0	31.0	41.0
46-47	37.40060227757921	40.0	37.0	41.0	31.0	41.0
48-49	37.27994271988798	40.0	36.5	41.0	30.5	41.0
50-51	37.14400885912613	40.0	36.0	41.0	30.0	41.0
52-53	37.02057618720317	40.0	36.0	41.0	30.0	41.0
54-55	36.84293229385534	39.0	35.0	41.0	30.0	41.0
56-57	36.70630435354484	39.0	35.0	41.0	30.0	41.0
58-59	36.48321679901844	39.0	35.0	41.0	30.0	41.0
60-61	36.294919897554124	38.5	35.0	40.0	30.0	41.0
62-63	36.06986544704726	38.0	35.0	40.0	29.5	41.0
64-65	35.8150822676503	37.0	35.0	40.0	29.0	41.0
66-67	35.548653526146694	37.0	34.5	40.0	29.0	41.0
68-69	35.26007241630743	36.0	34.0	39.5	29.0	41.0
70-71	34.993179100178814	36.0	34.0	39.0	29.0	41.0
72-73	34.6875717940641	35.0	34.0	39.0	29.0	40.5
74-75	34.38223817383556	35.0	34.0	37.5	28.0	40.0
76-77	34.08487432708347	35.0	34.0	37.0	28.0	39.0
78-79	33.810324382697516	35.0	34.0	37.0	28.0	39.0
80-81	33.53595925638381	35.0	33.5	36.0	28.0	39.0
82-83	33.290997572407875	35.0	33.0	36.0	27.0	37.0
84-85	33.07445284744617	35.0	33.0	35.5	27.0	37.0
86-87	32.89146474392916	35.0	33.0	35.0	27.0	37.0
88-89	32.74202263819029	35.0	33.0	35.0	27.0	36.0
90-91	32.58044914838665	35.0	33.0	35.0	26.5	36.0
92-93	32.49147391738093	35.0	33.0	35.0	26.0	36.0
94-95	32.39773692294098	35.0	33.0	35.0	26.0	35.5
96-97	32.31890999157527	35.0	33.0	35.0	26.0	35.0
98-99	32.25735124000111	35.0	33.0	35.0	25.0	35.0
100	31.302576525811464	34.0	31.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.25239326293482733
1101	2	0.25933241053615674
1101	3	0.3151098582562071
1101	4	0.37086423329181883
1101	5	0.3873400807814278
1101	6	0.34936889400417925
1101	7	0.3105666150414308
1101	8	0.3488864727400056
1101	9	0.4935411507670935
1101	10-11	0.47390106597734416
1101	12-13	0.4657114540695275
1101	14-15	0.4913734935381697
1101	16-17	0.4926749773085888
1101	18-19	0.4319354094837351
1101	20-21	0.4387868118358824
1101	22-23	0.4599016215601779
1101	24-25	0.4425021425785829
1101	26-27	0.4780222251150974
1101	28-29	0.5904621687816132
1101	30-31	0.48397299232838265
1101	32-33	0.5014274907679805
1101	34-35	0.4666743530605828
1101	36-37	0.45666790791938894
1101	38-39	0.4372932789863384
1101	40-41	0.46689720389591116
1101	42-43	0.4058004968202127
1101	44-45	0.3516114927142979
1101	46-47	0.3490884034363049
1101	48-49	0.3298517889680497
1101	50-51	0.4536088928076083
1101	52-53	0.4054681341319579
1101	54-55	0.4695490543393248
1101	56-57	0.4718736887589259
1101	58-59	0.38392877341014753
1101	60-61	0.37252643504355376
1101	62-63	0.3651323364325805
1101	64-65	0.38089045675224753
1101	66-67	0.3458617831964048
1101	68-69	0.2931621426396305
1101	70-71	0.28737761122850003
1101	72-73	0.20797209051614374
1101	74-75	0.26760388016830916
1101	76-77	0.28424479263969005
1101	78-79	0.27487091163867206
1101	80-81	0.24320556968897478
1101	82-83	0.20784856887334513
1101	84-85	0.281736623152689
1101	86-87	0.2368065991008379
1101	88-89	0.18839182018140832
1101	90-91	0.2787527389638882
1101	92-93	0.22787825265783113
1101	94-95	0.2661781086316708
1101	96-97	0.2610163079658463
1101	98-99	0.2949740693513263
1101	100	0.24732761900212807
1102	1	0.26014132957784497
1102	2	0.22206087410847175
1102	3	0.28995189834633806
1102	4	0.2592246815416388
1102	5	0.18974950655358924
1102	6	0.16714391883266444
1102	7	0.26003826509798245
1102	8	0.33648403442805375
1102	9	0.461074353345829
1102	10-11	0.44619296641945994
1102	12-13	0.4435733883857793
1102	14-15	0.48043334216703926
1102	16-17	0.4956417471388974
1102	18-19	0.43130935112771596
1102	20-21	0.4098538829540388
1102	22-23	0.40479762524292795
1102	24-25	0.4531655748746317
1102	26-27	0.43645348160723785
1102	28-29	0.4675053330446701
1102	30-31	0.45234023105653165
1102	32-33	0.4422145332455045
1102	34-35	0.4388544392981757
1102	36-37	0.418439390311967
1102	38-39	0.4204488653993863
1102	40-41	0.37997419048859626
1102	42-43	0.36964811433103506
1102	44-45	0.29540888389158226
1102	46-47	0.2582148913072828
1102	48-49	0.334804365578826
1102	50-51	0.30501692203215924
1102	52-53	0.24414546687308558
1102	54-55	0.33992434800761373
1102	56-57	0.2793920961091061
1102	58-59	0.2903098599649354
1102	60-61	0.27707764756825526
1102	62-63	0.2620015252139467
1102	64-65	0.3297137530714238
1102	66-67	0.349241574123063
1102	68-69	0.3186071766655516
1102	70-71	0.3525581080249154
1102	72-73	0.31698820781696213
1102	74-75	0.29245570863918147
1102	76-77	0.23018314756300384
1102	78-79	0.28200347734528464
1102	80-81	0.2509109344366891
1102	82-83	0.2560972582354779
1102	84-85	0.3388903779040362
1102	86-87	0.33445406196738503
1102	88-89	0.28238459445405795
1102	90-91	0.2751019967536763
1102	92-93	0.26828139632683445
1102	94-95	0.1880914867914285
1102	96-97	0.217072954559697
1102	98-99	0.25043801633675855
1102	100	0.15395429204956912
1103	1	0.07331406151018882
1103	2	0.11544888025916222
1103	3	0.10517486092962969
1103	4	0.06599044268976684
1103	5	0.10697144402155345
1103	6	0.15099835245644755
1103	7	0.1808481062051044
1103	8	0.15273211233682105
1103	9	0.3570251478102051
1103	10-11	0.3149782047587735
1103	12-13	0.29813515158414816
1103	14-15	0.35793206077288175
1103	16-17	0.3268821366827197
1103	18-19	0.21052513790372984
1103	20-21	0.2466042673722768
1103	22-23	0.3121528277032084
1103	24-25	0.29850130014372667
1103	26-27	0.25731457848063854
1103	28-29	0.32193895682735274
1103	30-31	0.3243289547879513
1103	32-33	0.27127655272269635
1103	34-35	0.2836776069045328
1103	36-37	0.26467209149085136
1103	38-39	0.211966036081094
1103	40-41	0.27861591268234065
1103	42-43	0.25521961612498245
1103	44-45	0.2531228767157785
1103	46-47	0.17812775650461532
1103	48-49	0.18219032149887227
1103	50-51	0.2008395770808562
1103	52-53	0.19852783488948944
1103	54-55	0.16193869962216922
1103	56-57	0.17226754459706228
1103	58-59	0.13679883997518516
1103	60-61	0.12228523342165687
1103	62-63	0.12502048984747205
1103	64-65	0.1448085762390363
1103	66-67	0.08963111794674461
1103	68-69	0.1765005646716986
1103	70-71	0.10896507880912765
1103	72-73	0.1300328003336162
1103	74-75	0.07974427860330024
1103	76-77	0.1144732552001102
1103	78-79	0.1135001457204794
1103	80-81	0.1449396376658001
1103	82-83	0.07490812445792727
1103	84-85	0.10657361726846659
1103	86-87	0.08829824443791523
1103	88-89	0.09120058625313732
1103	90-91	0.10775191987003296
1103	92-93	0.1637196331284798
1103	94-95	0.060336489354206435
1103	96-97	0.07406936665606167
1103	98-99	0.010817309007180143
1103	100	0.06066879743142195
1104	1	0.10131001408515417
1104	2	0.1027542702973605
1104	3	0.14609493160929787
1104	4	0.06979156476045034
1104	5	0.11763476485727153
1104	6	0.1449953412591043
1104	7	0.14963209440377767
1104	8	0.15094561085034996
1104	9	0.2458207181199441
1104	10-11	0.28174406819113074
1104	12-13	0.18768967722585472
1104	14-15	0.22387970084198372
1104	16-17	0.1607859887448697
1104	18-19	0.1881868925278951
1104	20-21	0.15373517060899644
1104	22-23	0.20524253640654422
1104	24-25	0.18328019382987293
1104	26-27	0.1936767058361255
1104	28-29	0.1824747191208118
1104	30-31	0.16397316223604008
1104	32-33	0.12011440809253315
1104	34-35	0.1505295785089089
1104	36-37	0.11933029861996403
1104	38-39	0.13666924448497042
1104	40-41	0.155366946163511
1104	42-43	0.08202086990240076
1104	44-45	-0.03437881697223588
1104	46-47	0.025510460796219547
1104	48-49	0.00994657539056476
1104	50-51	0.013712590951477921
1104	52-53	0.053581825180572196
1104	54-55	0.08883723075742012
1104	56-57	0.04771874750385763
1104	58-59	0.0687110980335035
1104	60-61	0.07902140159411175
1104	62-63	0.07782815419455602
1104	64-65	0.033263609500984614
1104	66-67	0.07145148435412807
1104	68-69	0.08129446257107276
1104	70-71	-0.019759787839376486
1104	72-73	0.11957098575322078
1104	74-75	0.09077563326706439
1104	76-77	-0.022746790640901793
1104	78-79	0.04379656543133592
1104	80-81	0.04541723558749311
1104	82-83	0.11284635715556846
1104	84-85	0.06711497993943993
1104	86-87	0.06636743126184541
1104	88-89	0.032879915329175446
1104	90-91	0.09051289083279812
1104	92-93	-0.01712255505204041
1104	94-95	-0.03394229882763966
1104	96-97	-0.015328506261205632
1104	98-99	-0.13071597459833129
1104	100	-0.14531801244832465
1105	1	0.13927353767771322
1105	2	0.22300133130602617
1105	3	0.15955861161459595
1105	4	0.12724593485549462
1105	5	0.13451929587613876
1105	6	0.09833881372923514
1105	7	0.059591347806062345
1105	8	0.12708943222058622
1105	9	0.15150529232575138
1105	10-11	0.1113822598103269
1105	12-13	0.18181834049330092
1105	14-15	0.2360562549742653
1105	16-17	0.12668725318710017
1105	18-19	0.09109780768875453
1105	20-21	0.1133517675924196
1105	22-23	0.12547340814261787
1105	24-25	0.19929646798392042
1105	26-27	0.17874698430210856
1105	28-29	0.16309041167363603
1105	30-31	0.20971021836751902
1105	32-33	0.14483490849204372
1105	34-35	0.12916760887665646
1105	36-37	0.18047867362390946
1105	38-39	0.1461428255392363
1105	40-41	0.14990754101229697
1105	42-43	0.08715485726602168
1105	44-45	0.05438488925535978
1105	46-47	0.024089745732901235
1105	48-49	0.07569985693920955
1105	50-51	8.47424331396951E-4
1105	52-53	0.011940532589868269
1105	54-55	4.973906630567626E-5
1105	56-57	0.04388634384401513
1105	58-59	0.04424579583627519
1105	60-61	0.04913108989144632
1105	62-63	0.0898305439340703
1105	64-65	0.07244328151109869
1105	66-67	0.05065217501870478
1105	68-69	0.006371505902706076
1105	70-71	0.06406916343038205
1105	72-73	0.0905773218975483
1105	74-75	0.06394431872494977
1105	76-77	0.01648826418867344
1105	78-79	0.012985295738801028
1105	80-81	-0.01594415922940584
1105	82-83	0.04194366850040865
1105	84-85	0.08603171064613946
1105	86-87	0.035173874370805436
1105	88-89	0.023059404634281577
1105	90-91	-0.005841701224930773
1105	92-93	-0.013468979601341857
1105	94-95	0.02055614444875431
1105	96-97	-0.04056083135905908
1105	98-99	-0.07350176189775937
1105	100	-0.09051473047410141
1106	1	0.11442438954709289
1106	2	0.05481059811588551
1106	3	0.022921061103112095
1106	4	0.08532015033476625
1106	5	0.031152091027038864
1106	6	0.05253331480317058
1106	7	-4.2653599022202116E-4
1106	8	0.07699432500881898
1106	9	0.07792678109490936
1106	10-11	0.13785681976882813
1106	12-13	0.14411379172923944
1106	14-15	0.13437424525421449
1106	16-17	0.1800070750772349
1106	18-19	0.1521268313153925
1106	20-21	0.16309118975922843
1106	22-23	0.18608440371944823
1106	24-25	0.18038429653610422
1106	26-27	0.14423967606372656
1106	28-29	0.19194367580775662
1106	30-31	0.2079948756175085
1106	32-33	0.11789459623095411
1106	34-35	0.16181708419428276
1106	36-37	0.14944346120263674
1106	38-39	0.20418575575147457
1106	40-41	0.19165175721965966
1106	42-43	0.09605432120719826
1106	44-45	0.03472335033876561
1106	46-47	0.019293714297461406
1106	48-49	0.03972578161793905
1106	50-51	0.02773739575371792
1106	52-53	0.06883244938698141
1106	54-55	0.05838350849730034
1106	56-57	-0.0017605672193639066
1106	58-59	0.0443862951263867
1106	60-61	0.029163239769488314
1106	62-63	-0.011233723967933429
1106	64-65	-0.030474439374259532
1106	66-67	-0.014307074498937311
1106	68-69	0.033899014184768816
1106	70-71	-0.022052976527383805
1106	72-73	-0.09097534884037373
1106	74-75	-0.010144597416882561
1106	76-77	0.0022108059630383536
1106	78-79	0.0294640991171633
1106	80-81	0.03379076923540936
1106	82-83	0.06245827132540427
1106	84-85	0.07647508545046122
1106	86-87	-0.05526102497027097
1106	88-89	-0.07556614826170716
1106	90-91	-0.02330223628263184
1106	92-93	0.017341604602670202
1106	94-95	-0.038033367819629404
1106	96-97	-0.014930234140372534
1106	98-99	0.067326421691007
1106	100	-0.05963821968011729
1107	1	0.08880499428381228
1107	2	0.12925576234462
1107	3	0.14623162542381962
1107	4	0.11512049126194768
1107	5	0.09110608133426723
1107	6	0.12254067723839057
1107	7	0.11985233006210194
1107	8	0.024361756956039926
1107	9	0.08714933667798164
1107	10-11	0.16980738422218877
1107	12-13	0.1479640358171821
1107	14-15	0.19384575285101135
1107	16-17	0.11506777094896847
1107	18-19	0.08818585018799041
1107	20-21	0.08825659582073797
1107	22-23	0.061239086714657276
1107	24-25	0.1321301388606031
1107	26-27	0.19297247493880576
1107	28-29	0.2245234189405707
1107	30-31	0.1827820724974245
1107	32-33	0.11512525380917538
1107	34-35	0.15300595718895948
1107	36-37	0.16004415190076315
1107	38-39	0.16172318699120325
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2216	16-17	-0.25311191847757186
2216	18-19	-0.3142079098621906
2216	20-21	-0.2680171452085176
2216	22-23	-0.21179522813321938
2216	24-25	-0.2895733117405328
2216	26-27	-0.26712201591933393
2216	28-29	-0.24773815167327484
2216	30-31	-0.27501453518446084
2216	32-33	-0.29820569914581796
2216	34-35	-0.2856199051944657
2216	36-37	-0.20260109723698605
2216	38-39	-0.27073165034339297
2216	40-41	-0.2833648545534828
2216	42-43	-0.2408228429729249
2216	44-45	-0.11018738145844509
2216	46-47	-0.04089997559581349
2216	48-49	-0.03876695992484969
2216	50-51	-0.12010970999352821
2216	52-53	-0.061504262378036856
2216	54-55	-0.0689557292716998
2216	56-57	-0.09525483890512731
2216	58-59	-0.023677380801167658
2216	60-61	-0.08677083220302251
2216	62-63	-0.027309651183855976
2216	64-65	-0.08530829628137582
2216	66-67	-0.12721966360229686
2216	68-69	-0.05999220702148733
2216	70-71	-0.11323261108528726
2216	72-73	-0.0759293304245503
2216	74-75	-0.1296693696707436
2216	76-77	-0.1107957474448682
2216	78-79	-0.037606463299141524
2216	80-81	0.020882364777200735
2216	82-83	-0.030433876420467243
2216	84-85	-0.034590616859802026
2216	86-87	-0.026614804169796002
2216	88-89	-0.05867820967708326
2216	90-91	-0.0362407997742622
2216	92-93	-0.056855640970908894
2216	94-95	-0.0560766488857567
2216	96-97	-0.11563667832481883
2216	98-99	-0.11612795637773132
2216	100	-0.02893361843030462
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	75.0
1	36.0
2	30.0
3	34.0
4	37.0
5	54.0
6	128.0
7	567.0
8	1764.0
9	2701.0
10	3191.0
11	4328.0
12	5100.0
13	5866.0
14	6749.0
15	7584.0
16	8115.0
17	8640.0
18	9729.0
19	10712.0
20	11786.0
21	13337.0
22	15093.0
23	17189.0
24	20334.0
25	24852.0
26	28540.0
27	27266.0
28	28985.0
29	36272.0
30	42585.0
31	46151.0
32	58227.0
33	79769.0
34	120688.0
35	188630.0
36	355383.0
37	720153.0
38	813739.0
39	240310.0
40	349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.23449639936947	19.948259180603873	12.971482817235646	23.845761602791008
2	14.999819549927162	21.25573687883435	39.84668152316051	23.89776204807797
3	18.265903206613178	26.953117094606355	30.279234525160316	24.50174517362015
4	12.064338823072283	17.68676782681081	39.55435165360004	30.69454169651687
5	12.820491198908776	39.13576407557342	35.07598964633058	12.96775507918722
6	29.162495446511688	38.7020534545798	18.606396470540616	13.529054628367895
7	26.053813262353987	33.50236121894344	23.329052267896664	17.114773250805904
8	25.689356277024157	34.7458295952026	21.038705673639264	18.52610845413398
9	26.478623461407956	15.905738028376131	20.76670064211575	36.84893786810016
10-11	24.444414838719254	26.678678346462537	28.625730718016033	20.251176096802176
12-13	25.326071742360234	24.763542968348165	27.978373747645378	21.93201154164622
14-15	22.59502789479427	25.960701234366358	25.823170901381054	25.621099969458317
16-17	22.08945563299914	28.745850704454806	26.492873659210257	22.671820003335792
18-19	22.015486044380467	28.748714527450343	27.57260622160282	21.663193206566376
20-21	23.378872426399848	27.555791727075064	26.835899562496195	22.229436284028896
22-23	23.338597522261786	27.41802072679534	26.585626824935222	22.65775492600765
24-25	23.07571668419352	27.71784845330853	26.807992245463197	22.398442617034753
26-27	22.83494297533344	27.65850631293329	26.84464635937597	22.661904352357297
28-29	22.4934095673924	28.28413668705631	26.696800301122735	22.525653444428556
30-31	22.797549803141706	27.7333419229411	26.664374230771955	22.80473404314524
32-33	22.755235995016132	28.067628271506084	26.27875184753198	22.898383885945805
34-35	23.313198800293044	27.758688498490926	26.613202443127925	22.314910258088105
36-37	23.228352764799013	27.368303168981484	26.806298193208477	22.597045873011027
38-39	22.92432000569399	27.575008976975596	26.91723152602513	22.583439491305278
40-41	22.84633993025226	27.434251615047934	27.023766415064998	22.695642039634812
42-43	22.780601892286036	27.46002413344439	26.560940320495106	23.19843365377447
44-45	22.78160270813728	27.713522283787324	26.756906423088594	22.7479685849868
46-47	23.02402339134332	27.665823205018196	26.473264807205112	22.836888596433376
48-49	23.01489263829294	27.49919832746059	26.59731039495593	22.888598639290542
50-51	22.752856893444136	27.57541279358592	26.778915371516337	22.892814941453608
52-53	22.897565626274595	27.710395250476438	26.5467815453292	22.84525757791976
54-55	22.57075433105694	27.50573934524652	26.657788935483666	23.265717388212874
56-57	23.088603349768853	27.368462427620848	26.486872390689854	23.056061831920445
58-59	22.96869912870385	27.25385672956081	26.80096829426699	22.97647584746835
60-61	22.63665679473191	27.66015915205276	26.83906114729021	22.864122905925118
62-63	22.8813497851699	27.4138929481309	26.565621482369018	23.139135784330183
64-65	22.518686259271437	27.600923681250013	26.553457402886195	23.326932656592362
66-67	22.83221529042743	27.33661295584723	26.390462891018394	23.44070886270694
68-69	22.677856510963	27.29260448641948	26.743408466880215	23.2861305357373
70-71	22.850390154163826	27.126287475001202	26.59968268079898	23.42363969003599
72-73	22.93504934107037	27.098123607145247	26.869362686345855	23.097464365438526
74-75	22.75968195069878	27.80534241043097	26.559771898447437	22.87520374042281
76-77	22.960658718899033	27.142042588653258	26.498894206519374	23.398404485928335
78-79	22.823764866874583	27.330588817978036	26.638493885907156	23.20715242924022
80-81	22.94687563263378	27.454500978473583	26.620234158850124	22.978389230042513
82-83	23.088037474556593	26.97635336315184	27.172359950349723	22.763249211941844
84-85	23.15576660312748	27.38594153270019	26.585550176279078	22.872741687893257
86-87	22.691992899010625	27.668640063371807	26.676819291156086	22.962547746461482
88-89	23.040019405147223	27.16715550634547	26.379327445665922	23.41349764284138
90-91	22.961082425099143	27.0083010439984	26.706133596033855	23.324482934868602
92-93	23.329730127289665	27.112016860845063	26.670978368280725	22.887274643584558
94-95	22.62419562115608	27.115103479059748	26.992867247673953	23.26783365211022
96-97	22.658392456722083	26.996144995925363	26.98130053968033	23.364162007672224
98-99	22.619440407319992	27.486728093932978	26.487387978230565	23.406443520516465
100	22.844516113796836	26.868326681802696	26.393734629229176	23.893422575171293
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1640.0
1	1267.5
2	971.0
3	1041.0
4	974.0
5	842.0
6	724.0
7	694.5
8	734.0
9	718.0
10	626.0
11	551.5
12	555.0
13	559.5
14	588.5
15	653.5
16	732.0
17	862.0
18	1044.5
19	1239.5
20	1520.0
21	2155.5
22	3157.5
23	4524.0
24	6308.5
25	8958.0
26	12499.0
27	16480.5
28	20655.0
29	26102.0
30	32826.0
31	40164.0
32	49538.5
33	55995.5
34	59485.5
35	64202.0
36	70954.5
37	81234.5
38	86207.5
39	85332.0
40	84969.5
41	84954.5
42	87566.0
43	91162.0
44	93929.0
45	102235.5
46	112151.5
47	122629.5
48	137408.0
49	133580.0
50	118405.0
51	114546.5
52	112128.5
53	109149.0
54	105703.5
55	97120.0
56	88439.5
57	78383.0
58	67925.0
59	60994.0
60	50509.0
61	39242.5
62	31815.5
63	25340.5
64	18471.5
65	12801.0
66	9465.0
67	7258.5
68	5530.5
69	4116.5
70	3142.5
71	2366.0
72	1758.5
73	1361.5
74	1017.5
75	792.0
76	570.5
77	396.5
78	295.5
79	188.5
80	136.5
81	113.0
82	89.5
83	76.0
84	63.5
85	52.5
86	44.5
87	34.5
88	24.0
89	18.5
90	17.5
91	15.0
92	12.5
93	10.0
94	7.5
95	9.5
96	9.0
97	7.5
98	8.0
99	6.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.062291784566881545
2	0.009072273984023355
3	0.011129555445084411
4	0.009240903611979179
5	0.011197007296266742
6	0.010724844337990433
7	0.0028329777496578505
8	0.004654177731580755
9	0.006475377713503659
10-11	0.006273022159956669
12-13	0.005497325871359877
14-15	0.009358944351548257
16-17	0.009021685095636608
18-19	0.0072510740021004515
20-21	0.006391062899525747
22-23	0.002546307382132949
24-25	0.003507496261481148
26-27	0.0044855481036249305
28-29	0.00586831105286269
30-31	0.008600111025747046
32-33	0.010522488784443444
34-35	0.01193897765927237
36-37	0.017318262791063167
38-39	0.018532396112345105
40-41	0.018127685005251127
42-43	0.024518747904776875
44-45	0.02787447750109778
46-47	0.03050509969720864
48-49	0.032461203381496204
50-51	0.03289964041418134
52-53	0.030589414511186554
54-55	0.03735146259221511
56-57	0.03952678479284525
58-59	0.03719969592705487
60-61	0.03777303666210467
62-63	0.03343925522363999
64-65	0.03603615149415968
66-67	0.03773931073651351
68-69	0.040471110709397864
70-71	0.039982084788325975
72-73	0.03596869964297735
74-75	0.03827892554597215
76-77	0.038548732950701464
78-79	0.03730087370382836
80-81	0.04310173290550873
82-83	0.03868363665306612
84-85	0.034670251507717506
86-87	0.033186310781706245
88-89	0.030892947841507035
90-91	0.03348984411202673
92-93	0.0354459477963143
94-95	0.0386161848018838
96-97	0.03401259595868979
98-99	0.031095303395054022
100	0.028599584901307824
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2965078.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.54985191833891
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.6470302299802	40.657261282057725
2	15.17960905091729	17.471685061171343
3	5.9966552182716	10.353198594505947
4	2.9886286011990477	6.879805337516702
5	1.6973868995588801	4.884218235887098
6	1.0184364702736435	3.516652083149037
7	0.6479682569774292	2.610333406578466
8	0.4356541949633689	2.0057467526196024
9	0.3082596402021779	1.5966266981432193
>10	1.0649319647591682	9.246598106754247
>50	0.012558732845712971	0.47888445975220806
>100	0.0026949989917689626	0.21903349067266698
>500	1.8574105968774626E-4	0.07995649119178491
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	2.0235555354698932E-4
14-15	0.0	0.0	0.0	0.0	2.866703675249015E-4
16-17	0.0	0.0	0.0	3.3725925591164884E-5	4.55299995480726E-4
18-19	0.0	0.0	0.0	6.745185118232977E-5	5.227518466630558E-4
20-21	0.0	0.0	0.0	1.6862962795582444E-4	6.407925862321329E-4
22-23	0.0	0.0	0.0	4.7216295827630844E-4	6.407925862321329E-4
24-25	0.0	0.0	0.0	9.611888793481993E-4	6.407925862321329E-4
26-27	0.0	0.0	0.0	0.0016694333167626618	6.576555490277153E-4
28-29	0.0	0.0	0.0	0.005649092536520119	6.745185118232978E-4
30-31	0.0	0.0	0.0	0.016306485023328224	6.913814746188802E-4
32-33	0.0	0.0	0.0	0.03924011442532035	8.094222141879573E-4
34-35	0.0	0.0	0.0	0.07418017333776716	9.105999909614519E-4
36-37	0.0	0.0	0.0	0.12864754316749846	9.105999909614519E-4
38-39	0.0	0.0	0.0	0.20481754611514436	9.611888793481993E-4
40-41	0.0	0.0	0.0	0.2831122823750336	9.780518421437818E-4
42-43	0.0	0.0	0.0	0.3651337334127467	9.780518421437818E-4
44-45	0.0	0.0	0.0	0.461640469491865	0.001028640730530529
46-47	0.0	0.0	0.0	0.5761568498366654	0.0010960925817128588
48-49	0.0	0.0	0.0	0.696356048643577	0.0011466814700996062
50-51	0.0	0.0	0.0	0.8215298214751855	0.001214133321281936
52-53	0.0	0.0	0.0	0.9420831425008043	0.00151766665160242
54-55	0.0	0.0	0.0	1.0664474931182248	0.0015513925771935848
56-57	0.0	0.0	0.0	1.2096983620666977	0.0015513925771935848
58-59	0.0	0.0	0.0	1.3688510049314049	0.0015851185027847498
60-61	0.0	0.0	0.0	1.537446906961638	0.0015851185027847498
62-63	0.0	0.0	0.0	1.7003094016413733	0.0016525703539670795
64-65	0.0	0.0	0.0	1.8609965741204784	0.001720022205149409
66-67	0.0	0.0	0.0	2.0458820982112442	0.001753748130740574
68-69	0.0	0.0	0.0	2.253836155406367	0.001753748130740574
70-71	0.0	0.0	0.0	2.47018796807369	0.001753748130740574
72-73	0.0	0.0	0.0	2.686489191852626	0.001939240721491981
74-75	0.0	0.0	0.0	2.8990131119653513	0.0021078703494478053
76-77	0.0	0.0	0.0	3.129141965236665	0.0021753222006301353
78-79	0.0	0.0	0.0	3.3828621034589985	0.0021921851634257175
80-81	0.0	0.0	0.0	3.6535463822536878	0.0021921851634257175
82-83	0.0	0.0	0.0	3.9317178165296154	0.0022259110890168825
84-85	0.0	0.0	0.0	4.19951852868626	0.0022259110890168825
86-87	0.0	0.0	0.0	4.474907574100918	0.0023776777541771243
88	0.0	0.0	0.0	4.694446486736605	0.0025294444193373665
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	11930	0.0	28.8504	1
TAGTCCG	115	4.205942E-6	28.602915	5
TACATGG	12100	0.0	27.65201	2
ACATGGG	11875	0.0	26.988756	3
GTACTAG	1335	0.0	26.063437	1
GTGATCG	690	0.0	25.196957	8
CATGGGG	8030	0.0	24.462048	4
CTAACGC	465	0.0	24.254389	3
GTATAAG	1495	0.0	22.64501	1
TGATCGC	775	0.0	22.43342	9
ATGGGGG	5450	0.0	22.245045	5
GTATTAG	670	0.0	21.053663	1
CATGGGA	3810	0.0	19.73454	4
GCATCGT	1440	0.0	19.578716	8
TAGGCAT	1880	0.0	19.496061	5
TCTAACG	610	0.0	19.259377	2
GCTATTG	1765	0.0	19.168283	8
TAACGCC	690	0.0	18.38852	4
ATTTAGG	2040	0.0	18.208666	1
TACTAGG	1845	0.0	18.08398	2
>>END_MODULE