FastQCFastQC Report
Mon 16 Jan 2017
ERR1146487_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146487_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2965078
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130810.44116883265802787No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109840.3704455666933551No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT65000.21921851634257175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64120.21625063489054924No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG117300.027.1619111
TACATGG117300.026.7593462
ACATGGG114300.026.0381013
GTATCAA227150.025.7530141
GGTATCA165850.025.2548141
CATGGGG81200.024.3768674
CTAACGC3750.023.821783
GAGTACT144550.023.37650312-13
GTGATCG6250.022.5584878
GTACTAG14050.022.442341
ATCAACG261850.022.2110443
CAACGCA262350.022.1687155
AACGCAG269850.021.8050946
TATCAAC270150.021.6702082
TCAACGC269550.021.5591184
AGTACTT151000.021.25751312-13
GTACTTT156550.021.254414-15
ATGGGGG53150.020.788185
AGAGTAC218500.020.73453510-11
TCTAACG5600.020.1683732
Produced by FastQC (version 0.11.5)