##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146487_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2965078
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.463887627914005	34.0	31.0	34.0	31.0	34.0
2	32.56824575947075	34.0	31.0	34.0	31.0	34.0
3	32.68481638594331	34.0	31.0	34.0	31.0	34.0
4	36.15933375108513	37.0	35.0	37.0	35.0	37.0
5	36.19308395934272	37.0	37.0	37.0	35.0	37.0
6	36.28572806516389	37.0	37.0	37.0	35.0	37.0
7	36.26776226460147	37.0	37.0	37.0	35.0	37.0
8	36.2778388966496	37.0	37.0	37.0	35.0	37.0
9	38.081909818224005	39.0	38.0	39.0	37.0	39.0
10-11	38.047140412495054	39.0	38.0	39.0	36.0	39.0
12-13	38.028407009866186	39.0	38.0	39.0	35.0	39.0
14-15	39.45709404609255	41.0	39.0	41.0	36.5	41.0
16-17	39.45780330230773	41.0	39.0	41.0	36.5	41.0
18-19	39.52354609221073	41.0	39.5	41.0	37.0	41.0
20-21	39.55643005006951	41.0	40.0	41.0	37.0	41.0
22-23	39.51327132034975	41.0	39.0	41.0	36.5	41.0
24-25	39.49124137712397	41.0	39.0	41.0	36.0	41.0
26-27	39.408111860800965	41.0	39.0	41.0	36.0	41.0
28-29	39.316172120935775	41.0	39.0	41.0	36.0	41.0
30-31	39.203393300277426	41.0	39.0	41.0	35.5	41.0
32-33	39.06440758050884	41.0	39.0	41.0	35.0	41.0
34-35	38.97075591266064	41.0	39.0	41.0	35.0	41.0
36-37	38.87114504239011	40.5	38.5	41.0	35.0	41.0
38-39	38.759061313058204	40.0	38.0	41.0	35.0	41.0
40-41	38.66522921150809	40.0	38.0	41.0	35.0	41.0
42-43	38.55646343873585	40.0	38.0	41.0	35.0	41.0
44-45	38.388968350916905	40.0	38.0	41.0	34.5	41.0
46-47	38.133081153345714	40.0	38.0	41.0	34.0	41.0
48-49	37.898927616743975	40.0	38.0	41.0	33.0	41.0
50-51	37.73932287784672	40.0	37.0	41.0	33.0	41.0
52-53	37.601415207289655	40.0	37.0	41.0	33.0	41.0
54-55	37.45446831415565	40.0	36.5	41.0	33.0	41.0
56-57	37.255177772726384	40.0	36.0	41.0	33.0	41.0
58-59	37.042040715286404	39.0	35.5	41.0	32.0	41.0
60-61	36.846510951819816	39.0	35.0	41.0	32.0	41.0
62-63	36.59367814269979	39.0	35.0	41.0	32.0	41.0
64-65	36.29572898250906	38.0	35.0	41.0	31.0	41.0
66-67	36.03440449121406	37.0	35.0	40.0	31.0	41.0
68-69	35.72279970375147	37.0	35.0	40.0	31.0	41.0
70-71	35.4323130791163	36.0	35.0	39.0	31.0	41.0
72-73	35.11347981402176	36.0	35.0	39.0	31.0	41.0
74-75	34.79662052735206	35.0	35.0	38.5	30.5	40.5
76-77	34.496280199036924	35.0	35.0	37.0	30.0	39.5
78-79	34.2116949368617	35.0	35.0	37.0	30.0	39.0
80-81	33.96221954363426	35.0	34.0	36.5	30.0	39.0
82-83	33.7149004174595	35.0	34.0	36.0	30.0	37.0
84-85	33.515959782508254	35.0	34.0	36.0	29.5	37.0
86-87	33.34238020045341	35.0	34.0	35.0	29.0	37.0
88-89	33.19193845828002	35.0	34.0	35.0	29.0	36.0
90-91	33.10262579938875	35.0	34.0	35.0	29.0	36.0
92-93	33.02471064842139	35.0	34.0	35.0	29.0	36.0
94-95	32.95330814231531	35.0	34.0	35.0	29.0	36.0
96-97	32.901580498050976	35.0	34.0	35.0	29.0	35.5
98-99	32.854514113962594	35.0	34.0	35.0	29.0	35.0
100	32.29577097128642	35.0	33.0	35.0	26.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2933315353516619
1101	2	0.2734220773311904
1101	3	0.28428608779013587
1101	4	0.16954548664435976
1101	5	0.16256653232989038
1101	6	0.1645791147446829
1101	7	0.16873254854026243
1101	8	0.16073341424187504
1101	9	0.24173064547954226
1101	10-11	0.20975487323930508
1101	12-13	0.22062566447887377
1101	14-15	0.34048747573108784
1101	16-17	0.3104704616735745
1101	18-19	0.315256174308324
1101	20-21	0.30719627209317224
1101	22-23	0.3201252942716266
1101	24-25	0.2743177658663285
1101	26-27	0.3330405049174985
1101	28-29	0.3875268246145964
1101	30-31	0.39134359099256955
1101	32-33	0.39335435991556267
1101	34-35	0.4282760064536575
1101	36-37	0.45031270086202113
1101	38-39	0.4385295940112428
1101	40-41	0.38858347638881696
1101	42-43	0.4214739437399473
1101	44-45	0.4423308090479452
1101	46-47	0.484012687410484
1101	48-49	0.502235771572515
1101	50-51	0.4995211544404512
1101	52-53	0.5397468255886508
1101	54-55	0.4420012789991503
1101	56-57	0.5175044546494192
1101	58-59	0.5169526976834291
1101	60-61	0.4966827428129861
1101	62-63	0.5305968470726086
1101	64-65	0.48856292496663656
1101	66-67	0.47511396621958824
1101	68-69	0.5031817914345922
1101	70-71	0.4584166705320456
1101	72-73	0.46551832961378636
1101	74-75	0.5040552270429757
1101	76-77	0.5094457840931526
1101	78-79	0.4641108581322726
1101	80-81	0.36964774521590726
1101	82-83	0.3883260867485845
1101	84-85	0.4323164753793307
1101	86-87	0.4582131360822288
1101	88-89	0.4463426587506234
1101	90-91	0.4271618216360551
1101	92-93	0.3307033959905965
1101	94-95	0.3746379022943245
1101	96-97	0.3426332872596447
1101	98-99	0.35081805926612475
1101	100	0.35329398879642326
1102	1	0.26985507219112037
1102	2	0.32762555611225963
1102	3	0.2707797413980231
1102	4	0.21676197742823433
1102	5	0.19035899005535128
1102	6	0.1404778412298029
1102	7	0.1575849927819064
1102	8	0.16838373199659884
1102	9	0.2622802411337446
1102	10-11	0.24515693688091744
1102	12-13	0.2595708723480499
1102	14-15	0.3489414728376161
1102	16-17	0.3332159668774679
1102	18-19	0.31257577026879346
1102	20-21	0.32232869721408264
1102	22-23	0.3324792966265093
1102	24-25	0.24469941422424313
1102	26-27	0.33164571125669795
1102	28-29	0.3627100751098524
1102	30-31	0.3368247725438849
1102	32-33	0.3099186342282252
1102	34-35	0.371875201472335
1102	36-37	0.3321660008955192
1102	38-39	0.3672542280398119
1102	40-41	0.35756645216180516
1102	42-43	0.3664279626359104
1102	44-45	0.29294119655043716
1102	46-47	0.3361998069807868
1102	48-49	0.39530368215731926
1102	50-51	0.4248761839344368
1102	52-53	0.42603090261179943
1102	54-55	0.415156324386615
1102	56-57	0.3976134193625356
1102	58-59	0.36314030215297777
1102	60-61	0.3463479208272702
1102	62-63	0.3958750320437261
1102	64-65	0.43440086634941366
1102	66-67	0.3958488260366195
1102	68-69	0.3425711934583049
1102	70-71	0.344174900361061
1102	72-73	0.43050651947032037
1102	74-75	0.42255575909643994
1102	76-77	0.3832673163450906
1102	78-79	0.35183023827615045
1102	80-81	0.3374482562624763
1102	82-83	0.3213229678237397
1102	84-85	0.2992163879382588
1102	86-87	0.3795431577812778
1102	88-89	0.3134900129398446
1102	90-91	0.263249685811374
1102	92-93	0.24572581482292577
1102	94-95	0.2953769108736921
1102	96-97	0.3582887937331307
1102	98-99	0.37035265852738064
1102	100	0.332194665267302
1103	1	0.24807137152479442
1103	2	0.21870705278063696
1103	3	0.26104114580602555
1103	4	0.157305288550738
1103	5	0.12603300338184908
1103	6	0.12330715952811033
1103	7	0.1537408103113833
1103	8	0.15146932912627165
1103	9	0.23819003098510194
1103	10-11	0.21786324134016866
1103	12-13	0.26559342488521054
1103	14-15	0.2925601299365397
1103	16-17	0.29067368087029166
1103	18-19	0.3096285637080527
1103	20-21	0.3492379745077798
1103	22-23	0.29647212081922447
1103	24-25	0.27173683093361944
1103	26-27	0.31511572663342946
1103	28-29	0.34477055691405667
1103	30-31	0.26276019027838515
1103	32-33	0.27698680439736734
1103	34-35	0.30011712868913776
1103	36-37	0.350746216169739
1103	38-39	0.30228754428789273
1103	40-41	0.37102109080865375
1103	42-43	0.35899587652622245
1103	44-45	0.3793071627216307
1103	46-47	0.32697376905152
1103	48-49	0.3243144458682252
1103	50-51	0.30288746020302426
1103	52-53	0.2972507898491159
1103	54-55	0.3208457144429957
1103	56-57	0.40017620767622475
1103	58-59	0.3632684415686569
1103	60-61	0.2936826209810377
1103	62-63	0.29361977832768105
1103	64-65	0.2920379755241882
1103	66-67	0.26578731911708786
1103	68-69	0.2986193738786014
1103	70-71	0.3467376886747502
1103	72-73	0.27750805714330795
1103	74-75	0.21291967503698572
1103	76-77	0.23014071460239194
1103	78-79	0.2329168605211578
1103	80-81	0.2837578410907682
1103	82-83	0.23393188632707052
1103	84-85	0.2492420158214017
1103	86-87	0.17631404450602872
1103	88-89	0.23289032047443925
1103	90-91	0.19418254075755925
1103	92-93	0.23329629150155284
1103	94-95	0.24540253875683504
1103	96-97	0.2912718793302531
1103	98-99	0.24131626693333175
1103	100	0.26838123625653765
1104	1	0.1505699354415242
1104	2	0.13843381749621386
1104	3	0.1403473909909323
1104	4	0.09162700327900097
1104	5	0.014667949560767113
1104	6	0.036154867521524636
1104	7	0.12897571363194515
1104	8	0.09125434787017639
1104	9	0.12017164027978566
1104	10-11	0.09547712441717238
1104	12-13	0.10950664163725321
1104	14-15	0.2079777924538746
1104	16-17	0.18916570187921877
1104	18-19	0.14770731726479625
1104	20-21	0.17776113917188496
1104	22-23	0.2086682719266335
1104	24-25	0.20485948399976905
1104	26-27	0.138643190392294
1104	28-29	0.18890889368906727
1104	30-31	0.14789338518705364
1104	32-33	0.15859087753236167
1104	34-35	0.19803553718686828
1104	36-37	0.1374476304323764
1104	38-39	0.1451379836491924
1104	40-41	0.16592020761585502
1104	42-43	0.1385182950205177
1104	44-45	0.16813072229127357
1104	46-47	0.17856865807249278
1104	48-49	0.16275346722783013
1104	50-51	0.14405238797456832
1104	52-53	0.11932528080510707
1104	54-55	0.033166917497531756
1104	56-57	0.06984564270988614
1104	58-59	0.03594740077985392
1104	60-61	0.10121807099188374
1104	62-63	0.09413663571656627
1104	64-65	0.08653054406946836
1104	66-67	0.10592223833589998
1104	68-69	0.20215192406826787
1104	70-71	0.13500480938239434
1104	72-73	0.09139662086935374
1104	74-75	0.06266801793768906
1104	76-77	0.04641246941723409
1104	78-79	0.12263564075261968
1104	80-81	0.08090781086641385
1104	82-83	0.10853311987493441
1104	84-85	0.1059239670358636
1104	86-87	0.10246343833303939
1104	88-89	0.11679411571201115
1104	90-91	0.0978692749595993
1104	92-93	0.053996101290756826
1104	94-95	0.030816402867046122
1104	96-97	0.07545959539780966
1104	98-99	0.0878826230726446
1104	100	0.041510069310859876
1105	1	0.19864169805047283
1105	2	0.15959675876465695
1105	3	0.1925294332691223
1105	4	0.11979324781198386
1105	5	0.10273928309861446
1105	6	0.0679938674500491
1105	7	0.12548537175437957
1105	8	0.14784649491284796
1105	9	0.1348972906985324
1105	10-11	0.10501098529809383
1105	12-13	0.1419936497022718
1105	14-15	0.194249120231909
1105	16-17	0.1870585604920194
1105	18-19	0.14974823245947988
1105	20-21	0.15935500891172438
1105	22-23	0.16804701427491153
1105	24-25	0.18964949625045335
1105	26-27	0.20502704906373026
1105	28-29	0.2565621848801527
1105	30-31	0.2296233858971206
1105	32-33	0.27865922692729583
1105	34-35	0.26974760202510595
1105	36-37	0.2419778051494319
1105	38-39	0.3203379185423145
1105	40-41	0.2586903070029507
1105	42-43	0.2770304349928452
1105	44-45	0.24633744292063398
1105	46-47	0.2494269238766762
1105	48-49	0.21212386950648465
1105	50-51	0.24971639475015905
1105	52-53	0.2148458658995196
1105	54-55	0.22784772998564762
1105	56-57	0.2770123369513655
1105	58-59	0.222828854845865
1105	60-61	0.17936722263785754
1105	62-63	0.15352106792082054
1105	64-65	0.18074164612111332
1105	66-67	0.16073228284387397
1105	68-69	0.1689287617372699
1105	70-71	0.16407516386858134
1105	72-73	0.1403685000387327
1105	74-75	0.15788297731526058
1105	76-77	0.1365444227739374
1105	78-79	0.16193890389668297
1105	80-81	0.15906897183954527
1105	82-83	0.16147562656197323
1105	84-85	0.1921268882872056
1105	86-87	0.2068512500552373
1105	88-89	0.17728824472506233
1105	90-91	0.18185799108906764
1105	92-93	0.20575741486396737
1105	94-95	0.16595628296266796
1105	96-97	0.15043883850005813
1105	98-99	0.14199324086857246
1105	100	0.1319119701891438
1106	1	0.09152640444676763
1106	2	0.12470151321512901
1106	3	0.12852774045176574
1106	4	0.1367719328751491
1106	5	0.13248827286889764
1106	6	0.11400596290310716
1106	7	0.12907680664461907
1106	8	0.07317368192365592
1106	9	0.09689217584178778
1106	10-11	0.14313128681980913
1106	12-13	0.14778580403988428
1106	14-15	0.15846611256872478
1106	16-17	0.1556588462687074
1106	18-19	0.17468094069420914
1106	20-21	0.1499762546796788
1106	22-23	0.20583085697031578
1106	24-25	0.181794050111165
1106	26-27	0.20252627546440038
1106	28-29	0.23571362605019885
1106	30-31	0.22460557035960704
1106	32-33	0.23879195609472248
1106	34-35	0.1930026804771785
1106	36-37	0.17225762057773153
1106	38-39	0.24212941532050536
1106	40-41	0.19539998427551097
1106	42-43	0.202658643154777
1106	44-45	0.09325618541216585
1106	46-47	0.1323883986300345
1106	48-49	0.17523125874525647
1106	50-51	0.14133279642113195
1106	52-53	0.1298052860640766
1106	54-55	0.22052561232573709
1106	56-57	0.17970304056271402
1106	58-59	0.18692673563875672
1106	60-61	0.19823138542135865
1106	62-63	0.23276704747959798
1106	64-65	0.2182285631912677
1106	66-67	0.12720829898934483
1106	68-69	0.16914724666573022
1106	70-71	0.15453207666118374
1106	72-73	0.10146538425367169
1106	74-75	0.14469202797236136
1106	76-77	0.13967761382566835
1106	78-79	0.1035900606552076
1106	80-81	0.11065103935201392
1106	82-83	0.11263511544034088
1106	84-85	0.12456766243261086
1106	86-87	0.1303745854514986
1106	88-89	0.1327127118516458
1106	90-91	0.0657254871755697
1106	92-93	0.0802277837343297
1106	94-95	0.07917072469697928
1106	96-97	0.0854630362320421
1106	98-99	0.14034234576864435
1106	100	0.1519731072891446
1107	1	0.10652631600910922
1107	2	0.08351587372240488
1107	3	0.11886703153547984
1107	4	0.0817188260601398
1107	5	0.0855672300120105
1107	6	0.07293938261055644
1107	7	0.055766573965136956
1107	8	0.038920670621322984
1107	9	0.08922485178850792
1107	10-11	0.09246057007678132
1107	12-13	0.09131418479054787
1107	14-15	0.13052652765087913
1107	16-17	0.13575141838961713
1107	18-19	0.0869592661713341
1107	20-21	0.17379030565515308
1107	22-23	0.13598727002725042
1107	24-25	0.1000450406129616
1107	26-27	0.16184086148771115
1107	28-29	0.20584969291791566
1107	30-31	0.14983324206132664
1107	32-33	0.1573958404978555
1107	34-35	0.19790887022902837
1107	36-37	0.13711017288861171
1107	38-39	0.13885672442781072
1107	40-41	0.1561829118273792
1107	42-43	0.16737986554011286
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2216	34-35	-0.5779507254012728
2216	36-37	-0.6146368316817998
2216	38-39	-0.5227957767504847
2216	40-41	-0.5630592124755154
2216	42-43	-0.6091749415390666
2216	44-45	-0.6261957571085617
2216	46-47	-0.5837716864492961
2216	48-49	-0.6165006010294007
2216	50-51	-0.6066146351366726
2216	52-53	-0.6602647412248857
2216	54-55	-0.5780890587865102
2216	56-57	-0.5563488397378364
2216	58-59	-0.5462671588371748
2216	60-61	-0.5738281405217478
2216	62-63	-0.5680489794576857
2216	64-65	-0.4880164067807371
2216	66-67	-0.53371110981751
2216	68-69	-0.48716994013683035
2216	70-71	-0.4628368424999252
2216	72-73	-0.4969854305263297
2216	74-75	-0.5090023585163905
2216	76-77	-0.4185261082259686
2216	78-79	-0.41806742233569594
2216	80-81	-0.41189944268528933
2216	82-83	-0.3753989974486984
2216	84-85	-0.44935138877730196
2216	86-87	-0.44833038945753856
2216	88-89	-0.43305141703204697
2216	90-91	-0.31424426502580616
2216	92-93	-0.3677116589554714
2216	94-95	-0.4594485622056723
2216	96-97	-0.41613822457195226
2216	98-99	-0.46668699155264903
2216	100	-0.6238728250667016
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	21.0
8	73.0
9	134.0
10	281.0
11	306.0
12	231.0
13	241.0
14	376.0
15	609.0
16	1193.0
17	2330.0
18	4083.0
19	6239.0
20	9151.0
21	13332.0
22	19296.0
23	24029.0
24	23807.0
25	20051.0
26	17232.0
27	17603.0
28	20612.0
29	24322.0
30	29966.0
31	37719.0
32	49299.0
33	64957.0
34	97139.0
35	158137.0
36	288765.0
37	703887.0
38	972425.0
39	356263.0
40	969.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.710603685854146	19.680576396597125	13.43322193002604	23.175597987522696
2	15.369199423182863	21.32738153104983	39.735571470235584	23.56784757553172
3	18.127442608398862	27.210674426353798	30.843879465833496	23.81800349941384
4	12.097995398434712	17.959089103220894	39.171144907486415	30.771770590857983
5	12.323446959102348	38.89951486397534	35.04255019803226	13.734487978890053
6	28.77606656690131	39.014528709859874	18.18505293668677	14.024351786552048
7	25.2248338829535	34.14753338698004	23.230114013864053	17.397518716202406
8	25.82869658066331	34.08234117281232	20.82818731918688	19.260774927337494
9	26.66931527602309	14.776440957033845	20.9949282953096	37.55931547163346
10-11	24.48778750508418	26.15732199962362	28.585723545889856	20.769166949402344
12-13	26.040512256338623	24.330017625168715	28.377111833145708	21.25235828534696
14-15	22.844806870918845	25.11680558663495	27.16166007858482	24.876727463861386
16-17	21.22743482633509	28.07430361022543	27.804428753644927	22.89383280979455
18-19	21.175125915743195	26.946491795494083	30.592416118564163	21.28596617019856
20-21	22.492747239701618	25.780721451509876	30.256033736717885	21.470497572070617
22-23	22.33909801670519	25.69667926447506	30.091589866802295	21.87263285201746
24-25	22.143059980209625	26.008523215915396	30.262087540361506	21.586329263513473
26-27	21.962676867185284	25.959451994180256	30.282238780902222	21.79563235773224
28-29	21.511732418235628	26.6349586435419	30.17407654106557	21.679232397156902
30-31	21.97592133076004	25.995789310379426	30.067831393667436	21.960457965193093
32-33	22.04932012652793	26.33820421127966	29.614571420188014	21.997904242004402
34-35	22.475051394658195	26.11079661340779	29.8970622879143	21.517089704019718
36-37	22.415797192534313	25.727620551162815	29.99547900994196	21.86110324636091
38-39	22.252142915802224	25.934631013431257	30.02151220860417	21.79171386216234
40-41	22.09050201363287	25.942491818825992	29.964479387960836	22.002526779580304
42-43	22.208840434150023	25.957738432098125	29.365125403955002	22.468295729796854
44-45	22.296580732054903	26.237877793214953	29.299210860337105	22.166330614393043
46-47	22.744340394044727	26.30673581175753	28.56118549666374	22.387738297534003
48-49	22.814136719393563	26.41664574240272	28.379558109899012	22.389659428304704
50-51	22.54522459475981	26.6888471996796	28.253552834046502	22.51237537151409
52-53	22.61427193226676	26.925608342676927	27.957684263316878	22.50243546173943
54-55	22.304111609848494	26.72742014196759	28.0395259023225	22.928942345861426
56-57	22.856281873440793	26.57114710154679	27.778311777182623	22.7942592478298
58-59	22.582297222779648	26.580287114904984	28.162758029891922	22.674657632423447
60-61	22.334711398820787	26.964670798839542	28.169719253726612	22.530898548613067
62-63	22.475896254613122	26.70306293575398	27.969771141946293	22.851269667686605
64-65	22.14108549407512	26.8623560432978	27.983537012715882	23.013021449911193
66-67	22.361719769852375	26.587332436976947	27.852304570726176	23.198643222444502
68-69	22.31033780306255	26.507901228405434	28.14383021549541	23.037930753036612
70-71	22.466174912724412	26.402016436242025	27.91186472460846	23.219943926425103
72-73	22.63123208647196	26.31953855358864	28.087636233877404	22.961593126061995
74-75	22.492681642731956	27.051758100530677	27.63565837221926	22.819901884518103
76-77	22.711240113336384	26.490069575534935	27.441830110769754	23.356860200358923
78-79	22.482693005888414	26.73495312149828	27.607785816030272	23.17456805658303
80-81	22.602023049987732	26.835487436669027	27.555569795393207	23.006919717950037
82-83	22.758313666866492	26.382322860570863	28.065688107120483	22.79367536544216
84-85	22.843439515600707	26.736309661022005	27.414977262797276	23.00527356058001
86-87	22.388123881458032	27.000382620819398	27.51733386980066	23.094159627921908
88-89	22.660824876824282	26.595618387810347	27.252398170297997	23.49115856506737
90-91	22.52344163010916	26.471061886240747	27.502552631423633	23.50294385222646
92-93	22.903276068960075	26.584983599082385	27.361354406191	23.15038592576654
94-95	22.19855973410476	26.604633437068674	27.686561312478343	23.510245516348224
96-97	22.281409587510026	26.431873691850367	27.695403744807027	23.59131297583258
98-99	22.305447055802066	26.83115572178038	27.200794047878617	23.662603174538933
100	22.440723536591495	26.252973393001866	27.206988624463623	24.099314445943016
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	584.0
1	391.0
2	578.0
3	866.5
4	510.5
5	385.0
6	880.0
7	2182.0
8	4159.5
9	5023.0
10	3498.5
11	1901.0
12	1712.5
13	1890.0
14	2151.0
15	2429.0
16	2832.5
17	3353.0
18	3851.5
19	4361.0
20	4955.0
21	5786.5
22	6897.5
23	8243.0
24	10035.5
25	12435.5
26	15699.5
27	19863.5
28	23753.0
29	28714.0
30	35064.0
31	41894.5
32	50453.5
33	55669.5
34	58841.0
35	63218.5
36	69366.5
37	79302.5
38	83898.0
39	83104.0
40	82747.5
41	82486.0
42	84945.5
43	88534.0
44	91337.5
45	99961.0
46	109923.0
47	120990.0
48	136616.0
49	131955.5
50	116409.0
51	112359.5
52	109252.5
53	106172.0
54	103540.5
55	95087.0
56	86043.0
57	76324.0
58	66145.5
59	59601.0
60	49245.0
61	37663.5
62	30043.5
63	23835.5
64	17165.0
65	11775.0
66	8602.5
67	6553.0
68	4984.5
69	3723.0
70	2780.0
71	2081.0
72	1560.0
73	1205.5
74	924.5
75	672.0
76	456.5
77	293.0
78	218.5
79	132.0
80	76.5
81	56.5
82	57.5
83	42.0
84	19.0
85	12.0
86	9.0
87	8.5
88	4.5
89	1.5
90	2.5
91	2.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0059020369784538555
2	0.08815956949530501
3	3.372592559116489E-4
4	0.0
5	0.03935815516488942
6	0.0040808369965309515
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	5.0588888386747336E-5
16-17	0.0
18-19	0.0
20-21	0.0
22-23	4.047111070939787E-4
24-25	0.0
26-27	0.0
28-29	1.0117777677349467E-4
30-31	1.8549259075140688E-4
32-33	0.0013827629492377603
34-35	0.0010960925817128588
36-37	6.576555490277153E-4
38-39	5.902036978453855E-4
40-41	0.0013153110980554305
42-43	6.745185118232978E-4
44-45	4.5529999548072595E-4
46-47	2.866703675249015E-4
48-49	6.576555490277153E-4
50-51	5.227518466630558E-4
52-53	0.0011635444328951887
54-55	0.001180407395690771
56-57	0.0010455036933261115
58-59	0.002124733312243388
60-61	0.001753748130740574
62-63	0.001028640730530529
64-65	0.004182014773304446
66-67	0.003760440703414885
68-69	0.0032376888567518295
70-71	0.00151766665160242
72-73	0.004721629582763084
74-75	0.0034569073730944004
76-77	0.0033388666335253236
78-79	6.576555490277153E-4
80-81	1.6862962795582442E-5
82-83	1.8549259075140688E-4
84-85	1.180407395690771E-4
86-87	5.0588888386747336E-5
88-89	1.6862962795582444E-4
90-91	1.6862962795582442E-5
92-93	0.0
94-95	1.6862962795582442E-5
96-97	4.047111070939787E-4
98-99	5.902036978453855E-4
100	0.0010455036933261115
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2965078.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.7096427205953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.02008549495154	35.85314404263242
2	16.254782964691955	17.135676051394636
3	6.6178731693269	10.464771909763037
4	3.3016391744155307	6.961128851030554
5	1.827741649934322	4.816980467679474
6	1.140726533917402	3.607637282681357
7	0.711601154006772	2.6255769811082184
8	0.49015346351679406	2.066865115218605
9	0.36211778152913465	1.7178388998457743
>10	1.2391759082840583	9.99116591959446
>50	0.021117604947015742	0.7356619062763405
>100	0.010388928645337624	0.9686840246814515
>500	0.00139793867946061	0.5255308647654287
>1k	7.98822102548919E-4	0.5831629425739554
>5k	1.9970552563722976E-4	0.6795848298298657
>10k+	1.9970552563722976E-4	1.2665899109243894
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13081	0.44116883265802787	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10984	0.3704455666933551	No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	6500	0.21921851634257175	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6412	0.21625063489054924	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.745185118232977E-5
2	0.0	0.0	0.0	0.0	6.745185118232977E-5
3	0.0	0.0	0.0	0.0	6.745185118232977E-5
4	0.0	0.0	0.0	0.0	6.745185118232977E-5
5	0.0	0.0	0.0	0.0	6.745185118232977E-5
6	0.0	0.0	0.0	0.0	6.745185118232977E-5
7	0.0	0.0	0.0	0.0	6.745185118232977E-5
8	0.0	0.0	0.0	0.0	6.745185118232977E-5
9	0.0	0.0	0.0	0.0	6.745185118232977E-5
10-11	0.0	0.0	0.0	0.0	6.745185118232977E-5
12-13	0.0	0.0	0.0	0.0	3.03533330320484E-4
14-15	0.0	0.0	0.0	0.0	3.541222187072313E-4
16-17	0.0	0.0	0.0	3.3725925591164884E-5	3.7098518150281375E-4
18-19	0.0	0.0	0.0	6.745185118232977E-5	3.7098518150281375E-4
20-21	0.0	0.0	0.0	2.0235555354698932E-4	4.0471110709397863E-4
22-23	0.0	0.0	0.0	5.058888838674733E-4	4.384370326851435E-4
24-25	0.0	0.0	0.0	9.949148049393642E-4	4.384370326851435E-4
26-27	0.0	0.0	0.0	0.001720022205149409	4.384370326851435E-4
28-29	0.0	0.0	0.0	0.005716544387702449	4.384370326851435E-4
30-31	0.0	0.0	0.0	0.016475114651284047	4.384370326851435E-4
32-33	0.0	0.0	0.0	0.03954364775564083	4.384370326851435E-4
34-35	0.0	0.0	0.0	0.07461861037045231	4.7216295827630844E-4
36-37	0.0	0.0	0.0	0.12922088390254827	4.7216295827630844E-4
38-39	0.0	0.0	0.0	0.2057281461061058	5.227518466630558E-4
40-41	0.0	0.0	0.0	0.28474798976620513	5.396148094586381E-4
42-43	0.0	0.0	0.0	0.36742709635294585	5.733407350498031E-4
44-45	0.0	0.0	0.0	0.46454089909270513	6.07066660640968E-4
46-47	0.0	0.0	0.0	0.5799847423912625	6.407925862321329E-4
48-49	0.0	0.0	0.0	0.700757281933224	6.576555490277153E-4
50-51	0.0	0.0	0.0	0.8267910658674071	7.419703630056275E-4
52-53	0.0	0.0	0.0	0.9480694942932362	7.756962885967924E-4
54-55	0.0	0.0	0.0	1.073597389343552	8.262851769835398E-4
56-57	0.0	0.0	0.0	1.2177588582829861	8.600111025747045E-4
58-59	0.0	0.0	0.0	1.3781762233573618	8.937370281658695E-4
60-61	0.0	0.0	0.0	1.547463506862214	9.611888793481993E-4
62-63	0.0	0.0	0.0	1.7111185607933417	0.0011466814700996062
64-65	0.0	0.0	0.0	1.8724127999330877	0.0011466814700996062
66-67	0.0	0.0	0.0	2.058664224010296	0.0011466814700996062
68-69	0.0	0.0	0.0	2.2679504552662695	0.0011466814700996062
70-71	0.0	0.0	0.0	2.4860560160643326	0.001180407395690771
72-73	0.0	0.0	0.0	2.7045156990811035	0.001214133321281936
74-75	0.0	0.0	0.0	2.918759641398978	0.001332174060851013
76-77	0.0	0.0	0.0	3.151350487238447	0.001416488874828925
78-79	0.0	0.0	0.0	3.4075494809917313	0.001483940726011255
80-81	0.0	0.0	0.0	3.6805102597638237	0.00151766665160242
82-83	0.0	0.0	0.0	3.960907605128769	0.0016188444283759145
84-85	0.0	0.0	0.0	4.2307655987464745	0.0016188444283759145
86-87	0.0	0.0	0.0	4.50768917377553	0.0016694333167626618
88	0.0	0.0	0.0	4.7292179160210965	0.0017200222051494093
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATG	11730	0.0	27.161911	1
TACATGG	11730	0.0	26.759346	2
ACATGGG	11430	0.0	26.038101	3
GTATCAA	22715	0.0	25.753014	1
GGTATCA	16585	0.0	25.254814	1
CATGGGG	8120	0.0	24.376867	4
CTAACGC	375	0.0	23.82178	3
GAGTACT	14455	0.0	23.376503	12-13
GTGATCG	625	0.0	22.558487	8
GTACTAG	1405	0.0	22.44234	1
ATCAACG	26185	0.0	22.211044	3
CAACGCA	26235	0.0	22.168715	5
AACGCAG	26985	0.0	21.805094	6
TATCAAC	27015	0.0	21.670208	2
TCAACGC	26955	0.0	21.559118	4
AGTACTT	15100	0.0	21.257513	12-13
GTACTTT	15655	0.0	21.2544	14-15
ATGGGGG	5315	0.0	20.78818	5
AGAGTAC	21850	0.0	20.734535	10-11
TCTAACG	560	0.0	20.168373	2
>>END_MODULE