##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146486_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3612282
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.582583530300237	31.0	31.0	34.0	27.0	34.0
2	30.69223194645379	31.0	31.0	34.0	27.0	34.0
3	30.92504073602227	31.0	31.0	34.0	27.0	34.0
4	34.40105838912909	37.0	35.0	37.0	30.0	37.0
5	34.65688669932192	37.0	35.0	37.0	31.0	37.0
6	34.794829694913076	37.0	35.0	37.0	32.0	37.0
7	34.66508539477261	37.0	35.0	37.0	31.0	37.0
8	34.62365756604828	37.0	35.0	37.0	31.0	37.0
9	36.01981240667257	39.0	35.0	39.0	30.0	39.0
10-11	35.93315305947874	39.0	35.0	39.0	30.0	39.0
12-13	35.92949678347372	39.0	35.0	39.0	30.0	39.0
14-15	36.971862246635226	40.0	36.0	41.0	30.0	41.0
16-17	36.80710545300727	40.0	36.0	41.0	29.5	41.0
18-19	36.756418657236615	40.0	36.0	41.0	29.5	41.0
20-21	37.09276116870167	40.0	36.0	41.0	30.0	41.0
22-23	36.79787084729266	40.0	36.0	41.0	29.5	41.0
24-25	36.921088525203736	40.0	36.0	41.0	30.0	41.0
26-27	36.99420546347157	40.0	36.0	41.0	30.0	41.0
28-29	36.819699430996806	40.0	36.0	41.0	30.0	41.0
30-31	36.76511745207046	40.0	36.0	41.0	30.0	41.0
32-33	36.87401066694129	40.0	36.0	41.0	30.0	41.0
34-35	36.93757782476562	40.0	36.0	41.0	30.0	41.0
36-37	36.880872119064904	40.0	35.5	41.0	30.0	41.0
38-39	36.70441593430414	40.0	35.0	41.0	30.0	41.0
40-41	36.670375264168186	39.0	35.0	41.0	30.0	41.0
42-43	36.51583735710557	39.0	35.0	41.0	29.0	41.0
44-45	36.32456588383742	39.0	35.0	41.0	29.0	41.0
46-47	36.19605570661427	39.0	35.0	41.0	28.5	41.0
48-49	36.06617312823307	39.0	35.0	41.0	28.0	41.0
50-51	35.84152150911806	38.0	34.0	40.0	28.0	41.0
52-53	35.65261114719172	38.0	34.0	40.0	28.0	41.0
54-55	35.456082886109115	38.0	34.0	40.0	28.0	41.0
56-57	35.304524536013524	37.0	34.0	40.0	28.0	41.0
58-59	35.027273756589324	37.0	33.5	40.0	27.0	41.0
60-61	34.76685555003735	36.5	33.0	40.0	27.0	41.0
62-63	34.50219044360324	36.0	33.0	39.0	26.0	41.0
64-65	34.21388612516963	35.0	33.0	39.0	26.0	41.0
66-67	33.92336021938486	35.0	33.0	39.0	26.0	41.0
68-69	33.638886858777916	35.0	32.0	38.0	26.0	40.0
70-71	33.59590931715741	35.0	32.0	37.5	26.0	40.0
72-73	33.39882918886178	35.0	32.0	37.0	26.0	40.0
74-75	33.13401542293764	35.0	32.0	36.5	26.0	39.5
76-77	32.91321455523129	35.0	32.0	36.0	25.0	39.0
78-79	32.70727700661244	35.0	32.0	36.0	25.0	39.0
80-81	32.49690721820721	35.0	32.0	35.5	25.0	38.0
82-83	32.260781688694294	35.0	32.0	35.0	25.0	37.0
84-85	32.092316159148154	35.0	31.5	35.0	25.0	37.0
86-87	31.926421580596422	35.0	31.5	35.0	24.5	36.5
88-89	31.93091347796213	35.0	32.0	35.0	25.0	36.0
90-91	31.858413601152954	35.0	32.0	35.0	25.0	36.0
92-93	31.716469672079867	35.0	32.0	35.0	24.0	36.0
94-95	31.715089519589	35.0	32.0	35.0	24.0	35.5
96-97	31.63968939855748	35.0	32.0	35.0	24.0	35.0
98-99	31.573377300000388	35.0	32.0	35.0	24.0	35.0
100	31.09663337469223	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8129157459605665
1101	2	0.9843848931092545
1101	3	0.9427180550172487
1101	4	1.0090331285321454
1101	5	0.941313989501765
1101	6	0.6596390502771712
1101	7	0.767486432051264
1101	8	0.7896469232491441
1101	9	0.9655588089153753
1101	10-11	1.0156705426669959
1101	12-13	0.9029919176225221
1101	14-15	1.097711411049275
1101	16-17	1.240259119694727
1101	18-19	1.3757091876380798
1101	20-21	1.0141503157268943
1101	22-23	1.3932918386835098
1101	24-25	1.212444496523311
1101	26-27	1.0813732615891283
1101	28-29	1.2978940113290918
1101	30-31	1.2328831824839455
1101	32-33	1.1519683642298375
1101	34-35	1.105690246707887
1101	36-37	1.009865082546483
1101	38-39	1.1055343107415823
1101	40-41	0.9984848203212593
1101	42-43	1.0511575167955627
1101	44-45	1.0951134307964878
1101	46-47	1.0901881520913648
1101	48-49	1.0160885912411501
1101	50-51	0.9978094618899149
1101	52-53	1.0635905619474926
1101	54-55	1.0648362111774219
1101	56-57	0.9689172227337153
1101	58-59	0.8567101398027646
1101	60-61	0.9374877622523456
1101	62-63	0.8790034340617865
1101	64-65	0.8612227591794124
1101	66-67	0.9289954154102915
1101	68-69	0.9589555098198659
1101	70-71	0.8295164322828299
1101	72-73	0.8246226805488419
1101	74-75	0.8284739400019419
1101	76-77	0.8146196195259137
1101	78-79	0.8037086241912803
1101	80-81	0.7913179901941092
1101	82-83	0.7763852185664604
1101	84-85	0.8141652136803685
1101	86-87	0.7733749306739774
1101	88-89	0.8076043574294332
1101	90-91	0.8554742300475162
1101	92-93	0.8778952813130907
1101	94-95	0.8566574004666663
1101	96-97	0.8018238135983999
1101	98-99	0.8126549421254836
1101	100	0.9491870418150086
1102	1	0.6870399571278547
1102	2	0.8368689857517388
1102	3	0.8490442857807992
1102	4	0.9070399420170361
1102	5	0.8005106948565981
1102	6	0.5959359650647826
1102	7	0.6452411573591021
1102	8	0.662957352755484
1102	9	0.8243389936836749
1102	10-11	0.8310733663190675
1102	12-13	0.7659075540398348
1102	14-15	0.9568068598074433
1102	16-17	1.1527051469302165
1102	18-19	1.1854957876306926
1102	20-21	0.8992786663438181
1102	22-23	1.2056580408153579
1102	24-25	1.0332454615747935
1102	26-27	0.8421703132307599
1102	28-29	1.1340517271278827
1102	30-31	1.1518360608776206
1102	32-33	1.007968490320053
1102	34-35	0.8523345169838024
1102	36-37	0.8161278453369505
1102	38-39	0.9104565576692849
1102	40-41	0.9029846073518044
1102	42-43	0.9501928689563215
1102	44-45	0.9669585612537404
1102	46-47	0.8955101972747173
1102	48-49	0.8652249885668013
1102	50-51	0.9543022432248733
1102	52-53	0.9079660540068843
1102	54-55	0.936873686881988
1102	56-57	0.8430985204788541
1102	58-59	0.8036092238034556
1102	60-61	0.7906263899909618
1102	62-63	0.8059088187292147
1102	64-65	0.693873206338381
1102	66-67	0.7935134547688918
1102	68-69	0.8435199911277564
1102	70-71	0.835928139144599
1102	72-73	0.7100847089406699
1102	74-75	0.7205279112287712
1102	76-77	0.7428069150137304
1102	78-79	0.7661201496061452
1102	80-81	0.7835145048759316
1102	82-83	0.767567514269544
1102	84-85	0.6459682038637808
1102	86-87	0.7048972740145736
1102	88-89	0.789159991663233
1102	90-91	0.7240366516255499
1102	92-93	0.7811935208168528
1102	94-95	0.7266710897127169
1102	96-97	0.7251227296838216
1102	98-99	0.7207943925258959
1102	100	0.792641191105826
1103	1	0.7732992624172041
1103	2	0.9324880245507465
1103	3	0.8325593999223138
1103	4	0.7821005496676037
1103	5	0.7776333217549549
1103	6	0.6148983368009766
1103	7	0.7275186111234504
1103	8	0.6916894090979966
1103	9	0.8158732032650917
1103	10-11	0.9111825754579712
1103	12-13	0.756145218209916
1103	14-15	0.9706706820814759
1103	16-17	1.1683711578436942
1103	18-19	1.246661945203627
1103	20-21	0.9364257038228914
1103	22-23	1.1600446992935431
1103	24-25	1.0788055047129035
1103	26-27	0.9121964875178179
1103	28-29	1.1411153235969351
1103	30-31	1.1570626607931658
1103	32-33	0.9512759201384497
1103	34-35	0.991480437448871
1103	36-37	0.9286335031045923
1103	38-39	0.9773543657426345
1103	40-41	0.8827618414430489
1103	42-43	0.89781604551154
1103	44-45	0.9936572190668898
1103	46-47	1.0236914272670816
1103	48-49	0.9518184656348225
1103	50-51	1.0022890814873122
1103	52-53	1.0369575458622222
1103	54-55	0.9682891133701972
1103	56-57	0.9658795851000335
1103	58-59	0.8997349725884547
1103	60-61	0.9057879982762103
1103	62-63	0.8636146508445961
1103	64-65	0.8626197611055773
1103	66-67	0.8641068676734065
1103	68-69	0.9104177201820036
1103	70-71	0.8787821336549797
1103	72-73	0.8347185344260382
1103	74-75	0.8410291167441812
1103	76-77	0.8365633550704743
1103	78-79	0.8357048836847412
1103	80-81	0.8583663756689504
1103	82-83	0.7676003862156193
1103	84-85	0.7401222285278521
1103	86-87	0.8761976076016076
1103	88-89	0.8817448155075382
1103	90-91	0.8939848103540591
1103	92-93	0.9522342766566787
1103	94-95	0.9788123888246645
1103	96-97	0.9576182208539059
1103	98-99	0.9975069972104009
1103	100	1.0848558815785267
1104	1	0.4574249782248998
1104	2	0.67870208880041
1104	3	0.6263655180265957
1104	4	0.66076063643672
1104	5	0.6022608954271149
1104	6	0.3732918514378767
1104	7	0.3776694348298335
1104	8	0.4199188952170019
1104	9	0.5651733670122567
1104	10-11	0.5456567182237393
1104	12-13	0.3895567487863687
1104	14-15	0.6228924291305589
1104	16-17	0.8126748384973297
1104	18-19	0.7946856313342607
1104	20-21	0.6352346448307742
1104	22-23	0.8397427892850757
1104	24-25	0.6529476515076382
1104	26-27	0.6130276778828048
1104	28-29	0.8101777819802081
1104	30-31	0.8169995815586688
1104	32-33	0.6462351896722822
1104	34-35	0.5673360398339895
1104	36-37	0.5384463661665748
1104	38-39	0.6189390931776941
1104	40-41	0.5608804183995275
1104	42-43	0.5281040942192874
1104	44-45	0.6069405841930617
1104	46-47	0.5517707086552406
1104	48-49	0.5673170504316616
1104	50-51	0.6246956771406076
1104	52-53	0.6410843683231633
1104	54-55	0.5925940507317549
1104	56-57	0.5229343869434615
1104	58-59	0.49053211929878415
1104	60-61	0.5535569665940443
1104	62-63	0.47379996836967564
1104	64-65	0.46732495055898227
1104	66-67	0.548752597961375
1104	68-69	0.49961607183139023
1104	70-71	0.4009261148681347
1104	72-73	0.40183999805934434
1104	74-75	0.45928054835957255
1104	76-77	0.47107645578154234
1104	78-79	0.4422073717788422
1104	80-81	0.4615048254916019
1104	82-83	0.3927270792543922
1104	84-85	0.41649730144504815
1104	86-87	0.3803172649517599
1104	88-89	0.39304160677585287
1104	90-91	0.3853528213230497
1104	92-93	0.35377614472485774
1104	94-95	0.30157999727199325
1104	96-97	0.35678213495511457
1104	98-99	0.45313592325032914
1104	100	0.517984315251546
1105	1	0.4884370487912868
1105	2	0.6204081842402722
1105	3	0.6183411414981599
1105	4	0.717717493643093
1105	5	0.6446980251693546
1105	6	0.33248365966887405
1105	7	0.4095723471972619
1105	8	0.4560531753639978
1105	9	0.46972408823840794
1105	10-11	0.5603443612592969
1105	12-13	0.5889300732269831
1105	14-15	0.7364211408624186
1105	16-17	0.8272882011243468
1105	18-19	0.8260154860428344
1105	20-21	0.6677310158143399
1105	22-23	0.9270526092074007
1105	24-25	0.7794341097183732
1105	26-27	0.5802119534526611
1105	28-29	0.8590914310052753
1105	30-31	0.8758840676768642
1105	32-33	0.7081047543210914
1105	34-35	0.6134312181462036
1105	36-37	0.5268431909406601
1105	38-39	0.6526108731612155
1105	40-41	0.5294985150948364
1105	42-43	0.6112913514675142
1105	44-45	0.6579372232661242
1105	46-47	0.5649766667809644
1105	48-49	0.684344003234834
1105	50-51	0.7241589750338164
1105	52-53	0.6914530240831596
1105	54-55	0.6724339816823601
1105	56-57	0.5831274113853766
1105	58-59	0.6465837494137432
1105	60-61	0.6504418973484789
1105	62-63	0.5562653455912141
1105	64-65	0.5827935840871703
1105	66-67	0.7030175750102998
1105	68-69	0.5841223294327094
1105	70-71	0.6082552895447932
1105	72-73	0.5464257670838961
1105	74-75	0.5720010683224288
1105	76-77	0.5423838171269608
1105	78-79	0.5878945309455403
1105	80-81	0.6899858696021397
1105	82-83	0.6282318067839157
1105	84-85	0.540947848739961
1105	86-87	0.5605845698022129
1105	88-89	0.5239963977314304
1105	90-91	0.5189477053906906
1105	92-93	0.5893321361983048
1105	94-95	0.526471780655676
1105	96-97	0.5547865028523695
1105	98-99	0.6060777151344112
1105	100	0.67072105135993
1106	1	0.4188025920010361
1106	2	0.46847237101027517
1106	3	0.4633117959400792
1106	4	0.551359357916887
1106	5	0.5178198580589921
1106	6	0.20989563156017965
1106	7	0.2585449956981236
1106	8	0.23468025550666027
1106	9	0.31440625511578446
1106	10-11	0.3396721886601455
1106	12-13	0.2715967501723
1106	14-15	0.4030229663514291
1106	16-17	0.4996853888845294
1106	18-19	0.526189537922626
1106	20-21	0.3510766835328525
1106	22-23	0.6154362736847148
1106	24-25	0.4309067839158658
1106	26-27	0.3231054631676997
1106	28-29	0.5791138213405986
1106	30-31	0.5597850396949369
1106	32-33	0.4088994656108227
1106	34-35	0.378056030132484
1106	36-37	0.3378362973898277
1106	38-39	0.42167015358688786
1106	40-41	0.3531491847491637
1106	42-43	0.332352314166279
1106	44-45	0.38453469745813607
1106	46-47	0.4215995463802358
1106	48-49	0.3988070977264968
1106	50-51	0.42757217351120147
1106	52-53	0.38553346140564315
1106	54-55	0.40124753374545463
1106	56-57	0.3802398804209304
1106	58-59	0.31003431079957977
1106	60-61	0.3513472754830431
1106	62-63	0.2515743390711265
1106	64-65	0.3259607629734447
1106	66-67	0.3242618403777513
1106	68-69	0.30422489327171576
1106	70-71	0.28055226042536674
1106	72-73	0.2700486843715595
1106	74-75	0.31773688292292235
1106	76-77	0.3183291107024644
1106	78-79	0.3866950976420185
1106	80-81	0.3667252748792009
1106	82-83	0.43518043406962903
1106	84-85	0.29841854600960005
1106	86-87	0.38753665756822286
1106	88-89	0.40170764977431617
1106	90-91	0.38465725802938877
1106	92-93	0.44175909779381684
1106	94-95	0.41519935177539224
1106	96-97	0.41189924598537075
1106	98-99	0.4420848690778172
1106	100	0.478320403119465
1107	1	0.45853347873697814
1107	2	0.56676210259727
1107	3	0.4364024369314343
1107	4	0.6595323594914717
1107	5	0.47546102881373287
1107	6	0.23381224592964855
1107	7	0.27762067913523936
1107	8	0.31304885638045477
1107	9	0.36009225318306903
1107	10-11	0.44125686043491896
1107	12-13	0.3133596081135508
1107	14-15	0.48499628911194037
1107	16-17	0.569642532970704
1107	18-19	0.6447607673724889
1107	20-21	0.3680695602210591
1107	22-23	0.6754497086735753
1107	24-25	0.40023047611357043
1107	26-27	0.38599521622933963
1107	28-29	0.5141876620934624
1107	30-31	0.48401968882798485
1107	32-33	0.41182005012120015
1107	34-35	0.3202497313935524
1107	36-37	0.37813404461996925
1107	38-39	0.3846451216250202
1107	40-41	0.24696318768224756
1107	42-43	0.32017705975513877
1107	44-45	0.3054052974075816
1107	46-47	0.2636320544515627
1107	48-49	0.2695146204532719
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2214	3	-1.0104396486068978
2214	4	-0.8622009516921239
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2214	16-17	-3.1015399143642313
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2214	94-95	-1.097617190455093
2214	96-97	-0.9888720178067878
2214	98-99	-1.2382427353812773
2214	100	-1.0943175427802032
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2215	3	-3.804249177900907
2215	4	-2.037706445122403
2215	5	-1.5637201068657163
2215	6	-1.384810561489303
2215	7	-1.289495102783441
2215	8	-1.225592623507744
2215	9	-1.8530316772065447
2215	10-11	-1.4244229848006
2215	12-13	-1.4002772942314223
2215	14-15	-3.7339805919416165
2215	16-17	-3.530705443192801
2215	18-19	-2.3111719950441625
2215	20-21	-1.7868692966475521
2215	22-23	-3.265166043556931
2215	24-25	-5.537921439004428
2215	26-27	-3.5242842557343934
2215	28-29	-1.83328657002329
2215	30-31	-4.071870382411198
2215	32-33	-4.043970072910966
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2215	36-37	-1.9955769988296055
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2215	44-45	-3.4978667091795828
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2215	100	-2.990582122132448
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2216	2	-0.4034364657481504
2216	3	-0.6802700210352448
2216	4	-0.6416825134713307
2216	5	-0.7793898220127886
2216	6	-0.5786361145860539
2216	7	-0.8087184048483138
2216	8	-0.6028751743869663
2216	9	-0.7165414409913566
2216	10-11	-0.7706334439114357
2216	12-13	-0.9415896500654313
2216	14-15	-1.1887918018744443
2216	16-17	-0.9045483442268392
2216	18-19	-2.9266694472739516
2216	20-21	-1.2460594266085465
2216	22-23	-0.7788790066455746
2216	24-25	-1.0111423983038677
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2216	28-29	-0.888394018905899
2216	30-31	-1.0681203955361127
2216	32-33	-1.0217493346222994
2216	34-35	-1.2338416701580428
2216	36-37	-1.1885328245891174
2216	38-39	-1.304412189918331
2216	40-41	-1.3331017493004467
2216	42-43	-1.2954017256884285
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2216	50-51	-1.228060253806035
2216	52-53	-1.1699224702060604
2216	54-55	-1.080526381530838
2216	56-57	-1.220343702232718
2216	58-59	-1.2436047320411987
2216	60-61	-1.264162275624976
2216	62-63	-1.2639603123660805
2216	64-65	-1.3393743119957549
2216	66-67	-1.1953389201333735
2216	68-69	-1.3252579678196028
2216	70-71	-1.4468968248551661
2216	72-73	-1.4022883005307278
2216	74-75	-1.44512393430918
2216	76-77	-1.351742280980961
2216	78-79	-1.5295228164917773
2216	80-81	-1.4397165659270108
2216	82-83	-1.5416970417027827
2216	84-85	-1.4696694147761775
2216	86-87	-1.3696014288634686
2216	88-89	-1.1989704918781001
2216	90-91	-1.322760177755594
2216	92-93	-1.3360418248596595
2216	94-95	-1.194627427102244
2216	96-97	-1.186008410769066
2216	98-99	-1.1556653336001332
2216	100	-1.1331195768002722
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	14840.0
1	2538.0
2	403.0
3	371.0
4	340.0
5	183.0
6	168.0
7	272.0
8	418.0
9	767.0
10	1150.0
11	2060.0
12	3162.0
13	4625.0
14	6320.0
15	8073.0
16	10017.0
17	12196.0
18	14074.0
19	16364.0
20	18888.0
21	22218.0
22	25646.0
23	30373.0
24	34454.0
25	38853.0
26	44407.0
27	51359.0
28	61233.0
29	73524.0
30	88237.0
31	106640.0
32	131857.0
33	169250.0
34	238412.0
35	323818.0
36	480581.0
37	744171.0
38	602823.0
39	226942.0
40	255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.79927945150392	21.96274658567231	12.630768254142257	23.607205708681512
2	15.175825511280413	19.931600315134666	41.46265089658483	23.429923277000093
3	18.058431332293466	27.594187078074523	29.295172506266898	25.052209083365117
4	11.893952584847334	17.24605601545607	41.106307204395165	29.75368419530143
5	12.785098774367572	38.919856335270346	35.38120730313319	12.913837587228889
6	28.737050952717162	39.35617384202037	18.6860197859829	13.220755419279564
7	26.11653194874978	32.12470712034575	24.50278279146134	17.25597813944313
8	25.722923966487304	35.376369546750524	20.266133849900708	18.634572636861463
9	26.023143309077994	14.869139174873297	22.27021714036371	36.837500375685
10-11	25.455887460923933	26.119985794568983	27.820142655480446	20.60398408902664
12-13	25.64584832169749	24.01057314576831	27.788434108483983	22.55514442405022
14-15	23.151220394645293	25.079541343129886	25.198992513428514	26.570245748796307
16-17	21.891147455935723	27.586365732001305	26.702443096036514	23.820043716026458
18-19	21.29551379185151	28.40309147212302	28.325608456669976	21.975786279355493
20-21	23.18944645899242	27.43195090718672	27.012054724827035	22.366547908993827
22-23	23.732857518640476	27.034719180257383	26.713552608901104	22.51887069220104
24-25	23.027350843624976	27.273988519742566	27.546119325100015	22.15254131153244
26-27	22.955830509875273	27.451274740358496	26.436694135355417	23.156200614410814
28-29	21.938302810404252	28.05044822631606	27.113591154655648	22.89765780862404
30-31	22.921793304535576	27.544456524673116	26.93483939495215	22.59891077583916
32-33	23.489798880102384	27.913231658625886	25.83205821363766	22.764911247634064
34-35	23.34982644107346	27.576693229309306	27.011791764912818	22.061688564704415
36-37	22.982762541856296	27.257581455709644	26.70455921484287	23.05509678759119
38-39	23.17668833118108	27.128266812404956	27.400649337003514	22.294395519410457
40-41	22.71761897803108	26.26109406205855	28.042713095760003	22.978573864150363
42-43	22.676541640142613	26.63393967296016	27.24791525811998	23.441603428777245
44-45	22.762379509209595	27.7322357595593	27.07755532895896	22.427829402272145
46-47	22.806690585740803	27.27500987503866	26.599276081027927	23.319023458192607
48-49	23.593355739493738	26.722323999535806	26.93581517227484	22.748505088695612
50-51	23.107086579765475	26.65498978597658	26.734355649526027	23.50356798473192
52-53	22.396442425278074	27.441497950807126	26.583233188296973	23.578826435617827
54-55	22.547505943200786	27.266453293163668	26.52787090485563	23.658169858779917
56-57	23.701851554739083	26.603090511903865	26.36522609851572	23.329831834841332
58-59	22.582557491954454	27.021239466615604	27.373342636657416	23.022860404772526
60-61	22.68125832911587	27.719311526083967	26.475404121709563	23.1240260230906
62-63	23.098785802085544	26.88682671374556	26.022074908978986	23.99231257518991
64-65	22.13903793918581	27.113159337956453	26.614304124948703	24.133498597909036
66-67	23.065933061267202	26.585811250746737	26.5151902043947	23.833065483591355
68-69	22.279277256707708	26.819325729284294	26.877073595891037	24.024323418116964
70-71	22.425736036292925	26.38058948032308	27.027619791551423	24.166054691832574
72-73	23.09252175311992	26.580981392998503	27.407490702483507	22.91900615139807
74-75	23.239792861714417	26.935018858902453	26.97032493980084	22.85486333958229
76-77	22.868141817894923	26.931020601662553	26.526381613248894	23.67445596719363
78-79	22.645427232621632	26.832297877286106	26.75231942386562	23.76995546622664
80-81	22.243407674604068	27.170231356365115	27.455873162625466	23.130487806405352
82-83	23.226061596666323	25.989960338410683	28.528318753271854	22.255659311651144
84-85	23.555240174247093	26.70163261318588	26.830236006222396	22.91289120634464
86-87	22.039528238724028	27.44981212527662	27.402124500438624	23.10853513556073
88-89	23.237923017742133	25.996518317190375	27.1629772475556	23.60258141751189
90-91	22.942181626905107	26.01579040633296	27.517930982548698	23.524096984213234
92-93	23.332675938960023	26.27548388821571	27.267748143397448	23.12409202942682
94-95	21.894611599862824	26.300753774119723	28.019008825526136	23.785625800491317
96-97	22.29836123671607	26.30692218694739	27.44170578514988	23.95301079118666
98-99	21.636381449575566	27.135811108700675	27.40951874427035	23.81828869745341
100	22.5477152894659	26.368045430257038	26.408604161062666	24.675635119214398
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12594.0
1	7393.5
2	2000.5
3	1397.5
4	717.5
5	383.5
6	319.5
7	317.5
8	310.5
9	290.5
10	258.5
11	241.0
12	233.0
13	229.5
14	253.5
15	380.5
16	451.0
17	493.0
18	621.0
19	773.5
20	1000.0
21	1291.0
22	1786.5
23	2552.0
24	3587.0
25	4804.0
26	6700.0
27	8904.5
28	12809.0
29	21519.0
30	32611.5
31	42869.0
32	56754.5
33	68649.5
34	77021.5
35	87785.0
36	98171.0
37	117219.0
38	124290.0
39	112743.0
40	102176.0
41	92045.0
42	89496.0
43	96065.5
44	105882.5
45	126986.0
46	147905.0
47	162827.5
48	172341.0
49	167961.5
50	159183.0
51	162502.0
52	164823.5
53	157505.0
54	148458.0
55	127280.5
56	109990.0
57	89884.5
58	69961.5
59	66470.0
60	54916.5
61	37521.5
62	27901.5
63	23613.0
64	17048.5
65	8043.5
66	4444.5
67	3007.0
68	2185.5
69	1666.5
70	1273.0
71	978.0
72	773.0
73	675.5
74	559.0
75	428.0
76	305.5
77	204.0
78	141.0
79	94.0
80	58.0
81	45.5
82	40.0
83	29.0
84	27.5
85	24.5
86	18.5
87	14.5
88	12.5
89	13.5
90	10.5
91	8.0
92	6.0
93	1.5
94	2.0
95	4.0
96	3.5
97	1.5
98	1.5
99	1.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.007534849161832
2	0.4528716196576015
3	0.48725431735396074
4	0.4722776350240651
5	0.4388638539294551
6	0.487669567326139
7	0.5046394495224902
8	0.5066326493889458
9	0.5217200650447557
10-11	0.5340114642212319
12-13	0.5319905810232978
14-15	0.5310216644215485
16-17	0.5348558058313276
18-19	0.5325304059871295
20-21	0.5329318142935685
22-23	0.4951302251596083
24-25	0.5303295811345847
26-27	0.5102730074783752
28-29	0.5153944238019069
30-31	0.5180935486210656
32-33	0.521235606743881
34-35	0.5333470642657467
36-37	0.5517564796989826
38-39	0.5409461387566087
40-41	0.5455554134477872
42-43	0.5434514802554175
44-45	0.5535282129136098
46-47	0.5506491464398405
48-49	0.5239070482315611
50-51	0.5378179222995325
52-53	0.5304126311290204
54-55	0.5404063137927769
56-57	0.5276027729839475
58-59	0.534315980867496
60-61	0.528433272928304
62-63	0.5226889816465049
64-65	0.5330563892852219
66-67	0.5304264727947596
68-69	0.5520471546795073
70-71	0.5372088890070045
72-73	0.5404201554585163
74-75	0.5375134056532684
76-77	0.5247513898416569
78-79	0.555452204451369
80-81	0.5300112228225814
82-83	0.5296651811790996
84-85	0.5385238472522356
86-87	0.8482172765027758
88-89	1.1196800249814383
90-91	1.1104614755990811
92-93	1.1100600672926422
94-95	1.1144478753319924
96-97	1.1077485091141832
98-99	1.1033745427405723
100	1.0986130097262616
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3612282.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.29854174605237
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.71938065467111	19.387122096399935
2	12.678975463725983	6.668771310599429
3	5.196388390711242	4.099723110654644
4	2.5090721548214927	2.6393975522972237
5	1.4307063656606245	1.88127455418344
6	0.9032394452641217	1.4252328154775813
7	0.5866747159919423	1.080008265690726
8	0.41462188777667214	0.8723160819617483
9	0.3155746976913415	0.746923892511024
>10	1.6377221018089743	8.216306126011034
>50	0.22522988383249973	4.223203935096891
>100	0.28758661403755115	16.81666240760611
>500	0.0560536621905623	10.461498582235107
>1k	0.03666668128254825	17.026639940080145
>5k	0.00136973234676186	2.1731496276187605
>10k+	7.375481867179246E-4	2.2817697015762692
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	14171	0.39230049038253384	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	12735	0.35254722637933583	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	11581	0.320600661853089	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	11159	0.30891829596914083	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	11147	0.30858609599139825	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	11066	0.3063437461416357	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	10488	0.2903427805470337	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	9612	0.2660921821718238	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	7033	0.19469687028864302	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	6286	0.1740174216741661	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6274	0.17368522169642348	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	6183	0.17116603853187543	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	6046	0.16737342211931405	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	5806	0.16072942256446202	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5332	0.14760752344362926	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	5297	0.14663860684188	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5181	0.1434273403903682	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5158	0.14279062376636154	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5125	0.14187707382756937	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	5094	0.14101889055173433	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4802	0.13293535775999768	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	4801	0.13290767442851914	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	4703	0.13019470794362123	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4675	0.12941957466222184	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4628	0.12811845808272998	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	4352	0.12047785859465016	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4331	0.11989650863360057	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	4278	0.11842929206523745	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	4255	0.11779257544123078	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	4147	0.11480277564154737	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	4146	0.11477509231006883	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	4068	0.11261579245474192	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	3984	0.1102903926105437	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	3915	0.10838024273852374	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	3915	0.10838024273852374	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	3914	0.10835255940704518	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	3900	0.10796499276634547	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	3811	0.10550117626475451	No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG	3779	0.10461530965744092	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	3729	0.10323114308351342	No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC	3641	0.10079500991340101	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	3624	0.10032439327826564	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	2.7683331478550123E-5	0.0
3	0.0	0.0	0.0	2.7683331478550123E-5	0.0
4	0.0	0.0	0.0	5.5366662957100246E-5	0.0
5	0.0	0.0	0.0	5.5366662957100246E-5	0.0
6	0.0	0.0	0.0	5.5366662957100246E-5	0.0
7	0.0	0.0	0.0	5.5366662957100246E-5	0.0
8	0.0	0.0	0.0	5.5366662957100246E-5	0.0
9	0.0	0.0	0.0	5.5366662957100246E-5	0.0
10-11	0.0	0.0	0.0	1.3841665739275063E-4	0.0
12-13	0.0	0.0	0.0	1.3841665739275063E-4	0.0
14-15	0.0	0.0	0.0	1.522583231320257E-4	0.0
16-17	0.0	0.0	0.0	1.6609998887130075E-4	0.0
18-19	0.0	0.0	0.0	1.6609998887130075E-4	0.0
20-21	0.0	0.0	0.0	1.799416546105758E-4	0.0
22-23	0.0	0.0	0.0	2.0762498608912594E-4	0.0
24-25	0.0	0.0	0.0	3.598833092211516E-4	0.0
26-27	0.0	0.0	0.0	6.920832869637531E-4	0.0
28-29	0.0	0.0	0.0	0.0018547832090628582	0.0
30-31	0.0	0.0	0.0	0.00656094956041638	0.0
32-33	0.0	0.0	0.0	0.01727439884261528	0.0
34-35	0.0	0.0	0.0	0.033303047768695804	0.0
36-37	0.0	0.0	0.0	0.06526345396068192	0.0
38-39	0.0	0.0	0.0	0.1166714005163495	0.0
40-41	0.0	0.0	0.0	0.17210727180214613	0.0
42-43	0.0	0.0	0.0	0.2315157011551147	0.0
44-45	0.0	0.0	0.0	0.3012638548153217	0.0
46-47	0.0	0.0	0.0	0.39142846544095944	0.0
48-49	0.0	0.0	0.0	0.5006668914553183	0.0
50-51	0.0	0.0	0.0	0.6190961835205557	0.0
52-53	0.0	0.0	0.0	0.7384528671903245	0.0
54-55	0.0	0.0	0.0	0.8690628251061241	0.0
56-57	0.0	0.0	0.0	1.0334741307572333	0.0
58-59	0.0	0.0	0.0	1.2337076673415863	0.0
60-61	0.0	0.0	0.0	1.440917403458534	0.0
62-63	0.0	0.0	0.0	1.6580377722448025	0.0
64-65	0.0	0.0	0.0	1.8898579900461812	0.0
66-67	0.0	0.0	0.0	2.158690822034382	0.0
68-69	0.0	0.0	0.0	2.462100135039291	0.0
70-71	0.0	0.0	0.0	2.7984249291721963	0.0
72-73	0.0	0.0	0.0	3.1488405390276837	0.0
74-75	0.0	0.0	0.0	3.4965570240640127	0.0
76-77	0.0	0.0	0.0	3.8954599890041806	0.0
78-79	0.0	0.0	0.0	4.323388926999609	0.0
80-81	0.0	0.0	0.0	4.7999021117398915	0.0
82-83	0.0	0.0	0.0	5.292097903762773	0.0
84-85	0.0	0.0	0.0	5.771185638330563	0.0
86-87	0.0	0.0	0.0	6.284932903909495	0.0
88	0.0	0.0	0.0	6.693691134856027	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	3855	0.0	39.3278	94
GGTATCA	5605	0.0	35.613377	1
GTACTAG	3970	0.0	33.04477	1
GCATCGT	4160	0.0	29.792402	8
TATTCTC	2035	0.0	29.78482	5
CAACGCA	7235	0.0	29.67887	5
TAGGCAT	5080	0.0	29.412579	5
TCTAACG	1970	0.0	29.339378	2
CTAACGC	1990	0.0	29.04451	3
GTATCAA	8435	0.0	28.867758	1
TAACGCC	2015	0.0	28.68216	4
CGCCTAC	2025	0.0	28.283276	7
TATTCGC	450	0.0	28.169712	9
TACCTGG	1365	0.0	27.884598	2
ATCAACG	7785	0.0	27.645145	3
CTAGGCA	5635	0.0	27.350277	4
TAGTACT	6035	0.0	26.861088	4
CTGTGCG	6065	0.0	26.784065	9
AGGGCTA	4845	0.0	26.572138	5
TACGGGT	90	9.536491E-4	26.104134	4
>>END_MODULE