##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146486_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3612282
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.867843097521177	31.0	31.0	34.0	30.0	34.0
2	31.874279472089942	31.0	31.0	34.0	30.0	34.0
3	32.045532713115975	33.0	31.0	34.0	30.0	34.0
4	35.767765639559705	37.0	35.0	37.0	35.0	37.0
5	35.676867974316515	37.0	35.0	37.0	33.0	37.0
6	35.702014958964995	37.0	35.0	37.0	33.0	37.0
7	35.62046678526206	37.0	35.0	37.0	33.0	37.0
8	35.60900117986359	37.0	35.0	37.0	33.0	37.0
9	37.2134473443657	39.0	37.0	39.0	33.0	39.0
10-11	37.1558069109776	39.0	37.0	39.0	33.0	39.0
12-13	37.0885826466483	39.0	37.0	39.0	33.0	39.0
14-15	38.28284045929969	40.0	38.0	41.0	33.0	41.0
16-17	38.28985970641273	40.0	38.0	41.0	33.0	41.0
18-19	38.34799345676777	40.0	38.0	41.0	33.5	41.0
20-21	38.36245329129896	40.0	38.0	41.0	33.5	41.0
22-23	38.330041231553906	40.0	38.0	41.0	33.0	41.0
24-25	38.256207156584125	40.0	37.5	41.0	33.0	41.0
26-27	38.1123761932208	40.0	37.0	41.0	33.0	41.0
28-29	38.049400766606816	40.0	37.0	41.0	33.0	41.0
30-31	37.9278328768352	40.0	37.0	41.0	33.0	41.0
32-33	37.80677034627972	40.0	36.5	41.0	33.0	41.0
34-35	37.71109343069007	40.0	36.0	41.0	32.5	41.0
36-37	37.631510219855485	40.0	36.0	41.0	32.0	41.0
38-39	37.51660210913766	40.0	36.0	41.0	32.0	41.0
40-41	37.391984208320395	40.0	36.0	41.0	31.0	41.0
42-43	37.27014225356713	39.5	36.0	41.0	31.0	41.0
44-45	37.05976249916258	39.0	35.0	41.0	31.0	41.0
46-47	36.822645075882775	39.0	35.0	41.0	30.5	41.0
48-49	36.56385907855477	39.0	35.0	41.0	30.0	41.0
50-51	36.36111757055512	39.0	35.0	41.0	30.0	41.0
52-53	36.16493092178296	38.0	35.0	40.5	29.0	41.0
54-55	35.96600957511069	38.0	35.0	40.0	29.0	41.0
56-57	35.69455388588156	37.5	34.0	40.0	28.5	41.0
58-59	35.399484038067904	37.0	34.0	40.0	28.0	41.0
60-61	35.1411577224591	36.5	34.0	40.0	28.0	41.0
62-63	34.86860286655361	36.0	34.0	39.5	28.0	41.0
64-65	34.588690888474375	35.5	33.5	39.0	27.0	41.0
66-67	34.370557725005966	35.0	33.0	39.0	27.0	41.0
68-69	34.18642744392603	35.0	33.0	38.5	27.0	41.0
70-71	33.99534961556158	35.0	33.0	38.0	27.0	40.5
72-73	33.80346592541778	35.0	33.0	37.0	26.5	40.0
74-75	33.62712587223257	35.0	33.0	37.0	26.5	39.5
76-77	33.47756307508661	35.0	33.0	36.0	27.0	39.0
78-79	33.36542122126678	35.0	33.0	36.0	27.0	39.0
80-81	33.22794967281071	35.0	33.0	36.0	27.0	38.5
82-83	33.0334359277598	35.0	33.0	35.5	27.0	37.0
84-85	32.89284405259612	35.0	33.0	35.0	27.0	37.0
86-87	32.75270521515209	35.0	33.0	35.0	27.0	36.5
88-89	32.64256278994829	35.0	33.0	35.0	27.0	36.0
90-91	32.54967095592204	35.0	33.0	35.0	27.0	36.0
92-93	32.46648019174583	35.0	33.0	35.0	26.0	36.0
94-95	32.42511451210066	35.0	33.0	35.0	27.0	35.5
96-97	32.371707690595585	35.0	33.0	35.0	26.0	35.0
98-99	32.32933945910092	35.0	33.0	35.0	26.0	35.0
100	31.828757555473242	35.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4236596427932042
1101	2	0.5684460673229879
1101	3	0.4606977643453263
1101	4	0.33235254341448695
1101	5	0.35044490626547287
1101	6	0.3741341353064769
1101	7	0.40531135645363037
1101	8	0.3957100658145123
1101	9	0.4800456535232982
1101	10-11	0.5301479544176857
1101	12-13	0.5072041754051497
1101	14-15	0.6041689182467778
1101	16-17	0.6384849927199099
1101	18-19	0.6324563580863085
1101	20-21	0.6598221402796938
1101	22-23	0.6244852393068285
1101	24-25	0.703304542195383
1101	26-27	0.6495827746091578
1101	28-29	0.6864535574358541
1101	30-31	0.7053984071145436
1101	32-33	0.7548914069883637
1101	34-35	0.7392529653308983
1101	36-37	0.7318858010519733
1101	38-39	0.7715099166265844
1101	40-41	0.789900402809181
1101	42-43	0.8189744321281722
1101	44-45	0.7747252561828475
1101	46-47	0.6768128715813972
1101	48-49	0.7140097016503262
1101	50-51	0.7374267478131031
1101	52-53	0.6688090591750893
1101	54-55	0.7088003136413477
1101	56-57	0.7201283318928873
1101	58-59	0.7450071563244265
1101	60-61	0.8070544383832825
1101	62-63	0.7503781381849777
1101	64-65	0.725371945829643
1101	66-67	0.6363944188381225
1101	68-69	0.6926679184338269
1101	70-71	0.6411321693442886
1101	72-73	0.6086668967144462
1101	74-75	0.6110383268737181
1101	76-77	0.5809120611115901
1101	78-79	0.5901813263280644
1101	80-81	0.5472382574110668
1101	82-83	0.5643829275403647
1101	84-85	0.5332987219506151
1101	86-87	0.4952703156640368
1101	88-89	0.5235158466383822
1101	90-91	0.5249268909320435
1101	92-93	0.5244475483022129
1101	94-95	0.5193620479409802
1101	96-97	0.5220537131896918
1101	98-99	0.5393193309566939
1101	100	0.6595578213130366
1102	1	0.4215598177818265
1102	2	0.5060303709359317
1102	3	0.42578492036506077
1102	4	0.34604386533204234
1102	5	0.30237896876931813
1102	6	0.30949794403068864
1102	7	0.31979224148395957
1102	8	0.3867768428870022
1102	9	0.4144051613408948
1102	10-11	0.4368090354414136
1102	12-13	0.47510521918613335
1102	14-15	0.6077631622282738
1102	16-17	0.5506388789127215
1102	18-19	0.5143380969934412
1102	20-21	0.5471709089010588
1102	22-23	0.5523095833301497
1102	24-25	0.5956867131921726
1102	26-27	0.6414170297850106
1102	28-29	0.5809794735889398
1102	30-31	0.6454842176593729
1102	32-33	0.6573753650833609
1102	34-35	0.6743135616761222
1102	36-37	0.6815741506232627
1102	38-39	0.6487753695980061
1102	40-41	0.6306615072057298
1102	42-43	0.7032549413481348
1102	44-45	0.6705617994348643
1102	46-47	0.6788960511461681
1102	48-49	0.6934816757926043
1102	50-51	0.6061462055328946
1102	52-53	0.6708636185666563
1102	54-55	0.6403867592503332
1102	56-57	0.6720821728777437
1102	58-59	0.649841735951938
1102	60-61	0.631547540325684
1102	62-63	0.5588727747376154
1102	64-65	0.5637295130880133
1102	66-67	0.6031049095443564
1102	68-69	0.586494033870963
1102	70-71	0.5814544567131179
1102	72-73	0.6269796558144662
1102	74-75	0.5955542942009799
1102	76-77	0.5623436810018632
1102	78-79	0.5510778470068587
1102	80-81	0.497809046305278
1102	82-83	0.5283330803863109
1102	84-85	0.5837097260900634
1102	86-87	0.5505679116463682
1102	88-89	0.5394360053890424
1102	90-91	0.5083267483885834
1102	92-93	0.4781210215341787
1102	94-95	0.444362009498839
1102	96-97	0.49025099210125944
1102	98-99	0.5107736201769058
1102	100	0.5601089662737309
1103	1	0.3245320264478835
1103	2	-0.17243735070948318
1103	3	0.23688501796945616
1103	4	0.2116906904077709
1103	5	0.25759908121666086
1103	6	0.18172619102037402
1103	7	0.28438575774545427
1103	8	0.3169772953819816
1103	9	0.3690954507151645
1103	10-11	0.4108942076019062
1103	12-13	0.3856946321437533
1103	14-15	0.44471526748677803
1103	16-17	0.5191880518671965
1103	18-19	0.4831274089962605
1103	20-21	0.49235235572184166
1103	22-23	0.4685725677488435
1103	24-25	0.4852177332465928
1103	26-27	0.5362507846589111
1103	28-29	0.5683492185026324
1103	30-31	0.5111837484755597
1103	32-33	0.5256087619059073
1103	34-35	0.4170668384402134
1103	36-37	0.5151173323494902
1103	38-39	0.5516055414297796
1103	40-41	0.5793967195711502
1103	42-43	0.5814149942213973
1103	44-45	0.6022930494664678
1103	46-47	0.5430083252779738
1103	48-49	0.5141489320471209
1103	50-51	0.5000804069595404
1103	52-53	0.4853335252482651
1103	54-55	0.5084386096880351
1103	56-57	0.5292201816343223
1103	58-59	0.4618396880929083
1103	60-61	0.40314488300813167
1103	62-63	0.45871037468096887
1103	64-65	0.40744174195767613
1103	66-67	0.44630604286791
1103	68-69	0.4150913310384112
1103	70-71	0.3621679565260081
1103	72-73	0.2821001567829242
1103	74-75	0.2952118570243272
1103	76-77	0.31555395051388757
1103	78-79	0.36840184460215397
1103	80-81	0.37559241512345665
1103	82-83	0.35113487961964296
1103	84-85	0.3276893793036777
1103	86-87	0.328081651487679
1103	88-89	0.333838813791111
1103	90-91	0.41298186636095124
1103	92-93	0.3801745146209541
1103	94-95	0.36814389844728623
1103	96-97	0.3608562657728527
1103	98-99	0.3416307846175499
1103	100	0.35315233005997726
1104	1	0.3480949621925973
1104	2	0.4270825118404318
1104	3	0.3664243688704403
1104	4	0.20809406738825942
1104	5	0.25898074716118913
1104	6	0.22810914180806918
1104	7	0.250135402772365
1104	8	0.3193328912022082
1104	9	0.3140997741655198
1104	10-11	0.2850635705635369
1104	12-13	0.3053908464518429
1104	14-15	0.4598179142637022
1104	16-17	0.42879145735808066
1104	18-19	0.4468047614315509
1104	20-21	0.49222417151471376
1104	22-23	0.44211592135831523
1104	24-25	0.43274239379478274
1104	26-27	0.47981642138778824
1104	28-29	0.4231731949890687
1104	30-31	0.4801448449250785
1104	32-33	0.48688992873106685
1104	34-35	0.44445643102300636
1104	36-37	0.45601013891588593
1104	38-39	0.4811744343451352
1104	40-41	0.4204751437719665
1104	42-43	0.411729602606826
1104	44-45	0.4151126143454249
1104	46-47	0.44120521201857343
1104	48-49	0.4216093129234082
1104	50-51	0.43531311362703207
1104	52-53	0.3763243550318691
1104	54-55	0.3458711741787184
1104	56-57	0.4078191023660622
1104	58-59	0.31439080515619366
1104	60-61	0.3917098538693935
1104	62-63	0.406756681554036
1104	64-65	0.38036913987829024
1104	66-67	0.3928403050396838
1104	68-69	0.39845940202927466
1104	70-71	0.42923575685574633
1104	72-73	0.4050005448618279
1104	74-75	0.3784682123087748
1104	76-77	0.4000864270350206
1104	78-79	0.38876822185086013
1104	80-81	0.34503017900867405
1104	82-83	0.26960473457303635
1104	84-85	0.30332130643712674
1104	86-87	0.2892256120683072
1104	88-89	0.25847471549779755
1104	90-91	0.30758967032952
1104	92-93	0.3415205988504013
1104	94-95	0.36330050863712415
1104	96-97	0.3665185977350589
1104	98-99	0.3159176966611241
1104	100	0.44370597554454605
1105	1	0.12229612086235164
1105	2	-0.37046100366298873
1105	3	0.0793902234220738
1105	4	0.10457751776554858
1105	5	0.1131693513956975
1105	6	0.18878394270257104
1105	7	0.2642251408293035
1105	8	0.21001933110439097
1105	9	0.38734078008964445
1105	10-11	0.31276650123950844
1105	12-13	0.3259832588650369
1105	14-15	0.3752195908039013
1105	16-17	0.36268974027751
1105	18-19	0.4574715850286566
1105	20-21	0.438074276788889
1105	22-23	0.39536320450006457
1105	24-25	0.4345967885009898
1105	26-27	0.41256492727922733
1105	28-29	0.42997743734198224
1105	30-31	0.4767446853791313
1105	32-33	0.42837135209016
1105	34-35	0.3724831615080504
1105	36-37	0.4088185399254769
1105	38-39	0.43856991213294094
1105	40-41	0.5066903738712227
1105	42-43	0.49165689150269287
1105	44-45	0.5115714789536838
1105	46-47	0.36734563860783
1105	48-49	0.4038191550775423
1105	50-51	0.4822413310279998
1105	52-53	0.40573730440871714
1105	54-55	0.4146566633439832
1105	56-57	0.4665110871438074
1105	58-59	0.4575649848677017
1105	60-61	0.4349504674674165
1105	62-63	0.5046253046201983
1105	64-65	0.4894969989547562
1105	66-67	0.4701175618955915
1105	68-69	0.4885118429515103
1105	70-71	0.38604745340458635
1105	72-73	0.40581370609478284
1105	74-75	0.4728307477816003
1105	76-77	0.3658340044213446
1105	78-79	0.34009781500869707
1105	80-81	0.41151977582821075
1105	82-83	0.3733723729006826
1105	84-85	0.36573332730887387
1105	86-87	0.4137010066829845
1105	88-89	0.4008370417300213
1105	90-91	0.3209787139692608
1105	92-93	0.30155407734438455
1105	94-95	0.33071390265022416
1105	96-97	0.2912027098357086
1105	98-99	0.2920302680971716
1105	100	0.2611273468550479
1106	1	0.22631438945729343
1106	2	0.27805630045155993
1106	3	0.20689333271500487
1106	4	0.1833508974213487
1106	5	0.15094426257098803
1106	6	0.1674302096666338
1106	7	0.17594400592881243
1106	8	0.16809646897883113
1106	9	0.1990421714768189
1106	10-11	0.2012908976255403
1106	12-13	0.2667824927626725
1106	14-15	0.3495907084705081
1106	16-17	0.3554181919352857
1106	18-19	0.3683588213383473
1106	20-21	0.3423572207325307
1106	22-23	0.26838430657738144
1106	24-25	0.288811465048866
1106	26-27	0.3130600157955641
1106	28-29	0.2946165537888561
1106	30-31	0.23159184178941672
1106	32-33	0.2794048707818959
1106	34-35	0.2365160086610345
1106	36-37	0.3269909437943568
1106	38-39	0.3131381229961079
1106	40-41	0.26345809684092814
1106	42-43	0.3178298572713487
1106	44-45	0.27361797615959915
1106	46-47	0.1911522868414437
1106	48-49	0.1962833596638731
1106	50-51	0.16956834984136293
1106	52-53	0.1576494767337877
1106	54-55	0.1904635377079913
1106	56-57	0.20808156273714218
1106	58-59	0.22897208125064594
1106	60-61	0.15213672010988688
1106	62-63	0.1244754418402394
1106	64-65	0.16210986714471431
1106	66-67	0.26660448916040025
1106	68-69	0.21264629460243611
1106	70-71	0.25763656762441656
1106	72-73	0.20817856748713126
1106	74-75	0.15639260986261405
1106	76-77	0.1526842649852327
1106	78-79	0.10916009337520904
1106	80-81	0.13697948864260923
1106	82-83	0.16473059428233938
1106	84-85	0.16491703084507492
1106	86-87	0.10517272153940382
1106	88-89	0.16338402374581307
1106	90-91	0.15685781301094437
1106	92-93	0.16255112394438243
1106	94-95	0.1012602473894404
1106	96-97	0.14071224671381088
1106	98-99	0.11042811711744349
1106	100	0.11902066812809409
1107	1	0.012060363128849616
1107	2	-0.5638944772705692
1107	3	-0.09210086046206101
1107	4	-0.041688500541759765
1107	5	0.029580096010477064
1107	6	0.10127207627976986
1107	7	0.130096948054657
1107	8	0.12949033323376113
1107	9	0.09432685385554151
1107	10-11	0.20934665566369404
1107	12-13	0.18582686457273923
1107	14-15	0.22392752887648726
1107	16-17	0.2394089304962037
1107	18-19	0.19737918089852968
1107	20-21	0.1488594685672311
1107	22-23	0.1850077127355334
1107	24-25	0.1535781984292015
1107	26-27	0.23116573905414128
1107	28-29	0.13273189061726498
1107	30-31	0.21388648386650289
1107	32-33	0.22662880660339368
1107	34-35	0.2669944763320373
1107	36-37	0.23186300295232343
1107	38-39	0.17466056833080756
1107	40-41	0.15956169997889447
1107	42-43	0.18686954400835276
1107	44-45	0.1769104487644313
1107	46-47	0.23855857370929812
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2216	76-77	-0.8384742883125753
2216	78-79	-0.7035602918427131
2216	80-81	-0.6543059367214141
2216	82-83	-0.6337730510355826
2216	84-85	-0.6513830363776378
2216	86-87	-0.5953039683330417
2216	88-89	-0.6481200052897904
2216	90-91	-0.6478390500249276
2216	92-93	-0.643637333366307
2216	94-95	-0.5891685931773054
2216	96-97	-0.6079702745132849
2216	98-99	-0.5948411488277543
2216	100	-0.6016912868413371
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	8.0
13	48.0
14	203.0
15	616.0
16	1442.0
17	3146.0
18	5465.0
19	8357.0
20	12194.0
21	17747.0
22	24182.0
23	28630.0
24	30499.0
25	31885.0
26	35310.0
27	43186.0
28	54535.0
29	67812.0
30	84057.0
31	104273.0
32	129965.0
33	164601.0
34	227616.0
35	314258.0
36	468722.0
37	802082.0
38	669307.0
39	281438.0
40	697.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.66147984137457	22.290095822584938	13.786280943546103	24.26214339249439
2	14.695077942474807	20.31936107851223	40.62739906320086	24.358161915812104
3	17.5943861498601	27.17511638999848	30.13779103036397	25.092706429777458
4	12.077841018282271	17.709978074122645	39.711064676076866	30.501116231518218
5	12.407673789211646	38.922321958522694	35.40650663087676	13.263497621388895
6	28.629575431818445	39.66514795910175	18.15126836719835	13.55400824188145
7	25.24341375840366	32.72329134213575	24.549389372042196	17.483905527418393
8	25.9512407946002	35.13011996294863	19.903429466470225	19.015209775980946
9	25.97419027639592	14.345834572162417	22.13824945007062	37.54172570137104
10-11	25.452442528019688	26.147252623134076	27.55500539548131	20.845299453364937
12-13	25.781749597622778	23.99663149222569	28.05805305344378	22.163565856707752
14-15	23.15349895195203	24.895852742269902	25.768566202273398	26.182082103504666
16-17	21.46473881511212	27.265471504271805	27.3265289342454	23.943260746370676
18-19	20.844967253387196	27.39739034770818	29.90361771312428	21.854024685780345
20-21	22.564665174811314	26.517569718076988	28.95943435178968	21.95833075532202
22-23	23.03735586919757	26.125494393117656	28.661111321813483	22.176038415871297
24-25	22.384658783561196	26.332620210714445	29.533699196242154	21.749021809482205
26-27	22.355856074080737	26.49135240257179	28.437023759265283	22.71576776408219
28-29	21.4363801054292	27.202873419074148	28.98450342470494	22.376243050791718
30-31	22.457825828659004	26.59378475988309	28.79376527081773	22.154624140640184
32-33	22.990743801286833	26.965059760007666	27.833859593464737	22.210336845240764
34-35	22.8131355956837	26.733325748056412	28.834682765171177	21.61885589108871
36-37	22.580753662089503	26.32151919479155	28.520281639141132	22.577445503977817
38-39	22.679306322153142	26.15062998957446	29.34660140044437	21.823462287828026
40-41	22.210021515568613	25.502772773227235	29.755419586808067	22.531786124396085
42-43	22.393607979948253	25.79151410725824	28.74896117493399	23.065916737859517
44-45	22.49381415958112	26.83975116007001	28.457385110021864	22.20904957032701
46-47	22.60887992687171	26.508616990589328	27.75825641519682	23.124246667342142
48-49	23.559068096647447	26.17166856344624	27.743134982142774	22.526128357763543
50-51	22.903115385664158	26.25307579157383	27.339356027485678	23.504452795276336
52-53	22.093443978072585	26.978375442448844	27.288927054975222	23.63925352450335
54-55	22.29460358871192	26.875295347442098	27.079917315173752	23.750183748672235
56-57	23.47728725789	26.153036096192473	26.93917426355692	23.430502382360608
58-59	22.263255150839846	26.605864725138588	27.96258590500446	23.168294219017103
60-61	22.47028192720387	27.293399182787603	27.02568737750119	23.210631512507334
62-63	22.911763256578528	26.45819180230115	26.533310522268195	24.09673441885213
64-65	21.94349721311902	26.67399721284219	27.123699091045495	24.2588064829933
66-67	22.72992529376167	26.192473345104283	27.06517652829984	24.012424832834203
68-69	22.138889777073135	26.133490355080376	27.512524436024922	24.21509543182157
70-71	22.19738700545421	25.75025178185144	27.63507217377294	24.417289038921407
72-73	22.8000333307311	25.88449628240542	27.981605533565762	23.333864853297722
74-75	22.90745386424175	26.37454966040782	27.441827502564863	23.276168972785563
76-77	22.582035617816494	26.396891517894588	26.914556607213203	24.10651625707571
78-79	22.206038174083176	26.359274333919068	27.292289399940618	24.142398092057135
80-81	21.839909395108258	26.834210599897514	27.90297347824727	23.422906526746964
82-83	22.832179196073774	25.586702589154264	28.944829525981515	22.636288688790447
84-85	23.236108498358917	26.294613522387237	27.13061117084105	23.3386668084128
86-87	21.63396670687976	27.02678496653177	27.705451885135897	23.633796441452574
88-89	22.64341136349668	25.70633567569633	27.53943636002217	24.110816600784823
90-91	22.298075936800217	25.55814609879461	27.963927604383027	24.179850360022144
92-93	22.71258737764154	26.01428108880463	27.356758254026637	23.916373279527196
94-95	21.23526187424389	25.958466099888433	28.235812780470788	24.57045924539689
96-97	21.624997646808687	25.899638882029258	27.708370938253367	24.766992532908688
98-99	20.921716879848393	26.743393355130667	27.665763573629537	24.669126191391406
100	21.861031211402715	25.939342591787078	26.690439869828275	25.50918632698193
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	191.0
1	129.5
2	100.5
3	126.0
4	86.0
5	71.5
6	141.5
7	335.0
8	575.5
9	657.5
10	520.0
11	395.5
12	393.0
13	432.0
14	574.5
15	875.5
16	1184.0
17	1587.5
18	2200.0
19	2907.5
20	3768.0
21	4665.0
22	5561.0
23	6626.0
24	7623.0
25	8705.0
26	10554.5
27	12346.0
28	15670.5
29	23713.5
30	34170.5
31	43290.5
32	56600.0
33	68276.5
34	74920.5
35	85116.0
36	95800.0
37	115190.5
38	122307.0
39	109963.0
40	100128.0
41	90602.5
42	87421.0
43	93075.5
44	102296.0
45	124484.5
46	146813.5
47	161911.5
48	172041.0
49	167759.5
50	159109.0
51	161781.5
52	162957.0
53	156920.5
54	150998.0
55	129817.5
56	111520.0
57	91199.5
58	70542.5
59	68711.5
60	56679.0
61	37697.0
62	27730.0
63	23312.0
64	16643.0
65	7274.0
66	3751.5
67	2560.5
68	1855.5
69	1457.0
70	1186.5
71	934.5
72	749.0
73	638.0
74	491.5
75	369.0
76	255.5
77	146.5
78	79.5
79	53.0
80	28.0
81	14.0
82	11.5
83	5.0
84	4.0
85	4.0
86	3.5
87	2.5
88	1.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013924715733710713
2	0.005398249638317275
3	0.0020208831979341593
4	0.028347731434035326
5	0.0024914998330695113
6	0.0
7	4.7061663513535217E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009107816056442992
16-17	0.0030174831311619637
18-19	0.0
20-21	4.1524997217825186E-5
22-23	6.920832869637531E-4
24-25	0.0
26-27	1.9378332034985087E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	5.813499610495525E-4
36-37	0.0
38-39	0.0
40-41	3.8756664069970173E-4
42-43	7.751332813994035E-4
44-45	0.0
46-47	0.0
48-49	5.259832980924524E-4
50-51	1.3841665739275062E-5
52-53	0.0
54-55	3.045166462640514E-4
56-57	9.689166017492543E-5
58-59	1.9378332034985087E-4
60-61	5.5366662957100246E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0012042249193169306
70-71	7.751332813994035E-4
72-73	0.0
74-75	2.2146665182840098E-4
76-77	0.0027960164793335626
78-79	4.014083064389768E-4
80-81	0.0031835831200332644
82-83	0.0016609998887130073
84-85	0.005273674646663799
86-87	0.00759907449086201
88-89	0.007197666184423032
90-91	0.005882707939191901
92-93	0.008374207772261413
94-95	0.0014395332368846066
96-97	0.00459543302543932
98-99	0.0018409415433235832
100	0.08017092796188116
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3612282.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.169088182021667
#Duplication Level	Percentage of deduplicated	Percentage of total
1	66.47823947732245	13.408054742036704
2	15.933766600769516	6.42739087285344
3	6.552233735156345	3.964577399807566
4	3.244264218066161	2.617354043998159
5	1.9275534148362725	1.9438497399694887
6	1.13280943375045	1.3708640017643312
7	0.7731039623528527	1.0914961393395533
8	0.5655493816341408	0.9125292279573449
9	0.39120035237039635	0.7101138963416825
>10	2.147540291342025	8.298056585737559
>50	0.3131891969804871	4.491963782977895
>100	0.4008890796276342	18.048279852704177
>500	0.08094293213837976	11.6043521888141
>1k	0.05515094698242146	19.63135526052478
>5k	0.002606636797676636	3.047408363800523
>10k+	9.603398728282344E-4	2.432353901372749
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	15819	0.4379226206591844	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	13549	0.37508145820287564	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	12200	0.3377366440383115	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	12028	0.3329751110240009	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	11846	0.32793674469490475	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	11806	0.3268294114357628	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	10657	0.29502126356690866	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	7029	0.19458613696272883	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6978	0.19317428705732276	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6477	0.17930493798656916	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	6415	0.17758857143489903	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6321	0.17498633827591534	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	6273	0.1736575383649449	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	6061	0.16778867209149229	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5771	0.15976050596271277	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	5677	0.15715827280372907	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5670	0.15696448948337918	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5566	0.15408542300960998	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	5511	0.15256283977828974	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	5476	0.1515939231765405	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	5301	0.1467493401677942	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5248	0.14528212359943105	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5222	0.14456235698098874	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5080	0.14063132391103464	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5049	0.13977314063519958	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5008	0.138638124044579	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4892	0.13542685759306722	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	4875	0.13495624095793185	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4832	0.1337658577043542	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	4663	0.12908737468447923	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4660	0.12900432469004358	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	4624	0.12800772475681577	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	4489	0.12427047500721151	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	4471	0.1237721750405976	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	4395	0.12166824184822779	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	4214	0.11665755885061022	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	4208	0.11649145886173892	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	4112	0.11383385903979812	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	4022	0.1113423592067286	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	3995	0.11059490925680775	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	3925	0.10865707605330925	No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC	3905	0.10810340942373822	No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG	3795	0.10505824296109773	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	3769	0.10433847634265542	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	3766	0.10425542634821977	No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT	3743	0.10361870972421311	No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT	3698	0.10237295980767835	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	3687	0.10206844316141431	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	3685	0.10201307649845721	No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT	3674	0.10170855985219315	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	2.7683331478550123E-5	0.0
10-11	0.0	0.0	0.0	1.1073332591420049E-4	0.0
12-13	0.0	0.0	0.0	1.1073332591420049E-4	0.0
14-15	0.0	0.0	0.0	1.1073332591420049E-4	0.0
16-17	0.0	0.0	0.0	1.1073332591420049E-4	0.0
18-19	0.0	0.0	0.0	1.1073332591420049E-4	0.0
20-21	0.0	0.0	0.0	1.3841665739275063E-4	0.0
22-23	0.0	0.0	0.0	1.799416546105758E-4	0.0
24-25	0.0	0.0	0.0	3.598833092211516E-4	0.0
26-27	0.0	0.0	0.0	7.474499499208534E-4	0.0
28-29	0.0	0.0	0.0	0.0019516748692377838	0.0
30-31	0.0	0.0	0.0	0.00672704954928768	0.0
32-33	0.0	0.0	0.0	0.017634282151836426	0.0
34-35	0.0	0.0	0.0	0.03400897272139883	0.0
36-37	0.0	0.0	0.0	0.06614932056799552	0.0
38-39	0.0	0.0	0.0	0.11816630041619121	0.0
40-41	0.0	0.0	0.0	0.17455724663799782	0.0
42-43	0.0	0.0	0.0	0.23526679257045824	0.0
44-45	0.0	0.0	0.0	0.3062468544814607	0.0
46-47	0.0	0.0	0.0	0.3977125816865904	0.0
48-49	0.0	0.0	0.0	0.5082936492776589	0.0
50-51	0.0	0.0	0.0	0.6287299828750912	0.0
52-53	0.0	0.0	0.0	0.7505089580492332	0.0
54-55	0.0	0.0	0.0	0.8825722908676565	0.0
56-57	0.0	0.0	0.0	1.0484369714213897	0.0
58-59	0.0	0.0	0.0	1.2513419494934226	0.0
60-61	0.0	0.0	0.0	1.461347702089704	0.0
62-63	0.0	0.0	0.0	1.6809180457118242	0.0
64-65	0.0	0.0	0.0	1.9161433132850645	0.0
66-67	0.0	0.0	0.0	2.1881735700590372	0.0
68-69	0.0	0.0	0.0	2.4948910411756335	0.0
70-71	0.0	0.0	0.0	2.834856193397968	0.0
72-73	0.0	0.0	0.0	3.190185594590899	0.0
74-75	0.0	0.0	0.0	3.542400620992492	0.0
76-77	0.0	0.0	0.0	3.948778085431868	0.0
78-79	0.0	0.0	0.0	4.386202406124439	0.0
80-81	0.0	0.0	0.0	4.873110681834918	0.0
82-83	0.0	0.0	0.0	5.375936873145562	0.0
84-85	0.0	0.0	0.0	5.86612562363625	0.0
86-87	0.0	0.0	0.0	6.389589738564155	0.0
88	0.0	0.0	0.0	6.805974727333027	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7670	0.0	41.311825	1
GGGCACC	3870	0.0	39.747086	94
GTATCAA	12950	0.0	37.391884	1
TACCCCG	245	0.0	34.529003	5
GTACTAG	3825	0.0	32.57049	1
CAACGCA	14125	0.0	32.37442	5
ATCAACG	14420	0.0	31.7491	3
TATTCTC	2285	0.0	31.263311	5
GTGGTAT	1870	0.0	29.665403	1
TATCAAC	16330	0.0	29.618761	2
AACGCAG	16600	0.0	28.67959	6
ACGCAGA	16975	0.0	26.938574	7
CGCAGAG	17075	0.0	26.753286	8
TCTAACG	1880	0.0	26.752455	2
CTGTGCG	6010	0.0	26.592562	9
CTAACGC	1895	0.0	26.540693	3
TAGGCAT	5310	0.0	26.463924	5
AGGGCTA	4915	0.0	26.295824	5
CTATTGA	4910	0.0	26.040133	9
TAACGCC	1955	0.0	25.966578	4
>>END_MODULE