FastQCFastQC Report
Mon 16 Jan 2017
ERR1146485_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146485_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2970727
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN91170.30689457496431005No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC74320.25017445224687423No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA74230.24987149610179596No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT65050.21896996930381016No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC61830.2081308716687868No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA59440.20008570292726324No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC55360.18635169101704735No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC49010.16497645189207893No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT39290.13225718822362337No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC37090.12485159356615402No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC35850.1206775311228531No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG35070.11805191119884123No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT33830.11387784875554031No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT32190.10835731455633586No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC30560.10287044215102903No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA30090.10128833783784238No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC29800.10031214581481235No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41100.035.124641
GTACTAG24850.032.274151
GGGCACC25100.031.41180294
GTATCAA62650.031.1753641
CAACGCA58050.031.0019235
ATCAACG61650.029.267813
TAGGCAT30550.028.7622245
GCATCGT25650.027.2737738
AACGCAG70450.026.3903926
CTAGGCA35500.026.0753384
GTACATG119900.026.0478021
TATCAAC74700.025.8540152
TACATGG120900.025.3412532
ACGCAGA71350.025.071337
ACATGGG117900.024.6699873
TAGTACT36400.024.656084
CGCAGAG72200.024.6461098
GCTATTG33550.024.3502318
CTGTGCG34700.024.2214079
TCTAACG12550.023.9631022
Produced by FastQC (version 0.11.5)