##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146485_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2970727
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.597040051138997	31.0	31.0	34.0	27.0	34.0
2	30.636841756243506	31.0	31.0	34.0	27.0	34.0
3	30.804190691369488	31.0	31.0	34.0	27.0	34.0
4	34.2615309316541	37.0	35.0	37.0	30.0	37.0
5	34.46055393174802	37.0	35.0	37.0	30.0	37.0
6	34.62288052722448	37.0	35.0	37.0	31.0	37.0
7	34.48087084407285	37.0	35.0	37.0	30.0	37.0
8	34.436493491323844	37.0	35.0	37.0	30.0	37.0
9	35.79560424098209	39.0	35.0	39.0	30.0	39.0
10-11	35.71915022821013	39.0	35.0	39.0	30.0	39.0
12-13	35.72927317117998	39.0	35.0	39.0	30.0	39.0
14-15	36.762068510502644	40.0	36.0	41.0	29.5	41.0
16-17	36.60171096166023	40.0	36.0	41.0	28.5	41.0
18-19	36.562921635007186	40.0	36.0	41.0	28.0	41.0
20-21	36.89336414958359	40.0	36.0	41.0	30.0	41.0
22-23	36.6171430427636	40.0	36.0	41.0	28.5	41.0
24-25	36.742760778758864	40.0	36.0	41.0	29.5	41.0
26-27	36.82088071371082	40.0	36.0	41.0	30.0	41.0
28-29	36.65364050617913	40.0	36.0	41.0	29.5	41.0
30-31	36.59437942294933	40.0	35.0	41.0	29.5	41.0
32-33	36.69785443091877	40.0	35.5	41.0	30.0	41.0
34-35	36.74519570462045	40.0	35.0	41.0	30.0	41.0
36-37	36.68418050531065	40.0	35.0	41.0	30.0	41.0
38-39	36.51688913185224	39.0	35.0	41.0	29.0	41.0
40-41	36.48256908157498	39.0	35.0	41.0	29.0	41.0
42-43	36.32399325148356	39.0	35.0	41.0	29.0	41.0
44-45	36.15628144221937	39.0	35.0	41.0	28.0	41.0
46-47	36.0294424563415	39.0	35.0	41.0	28.0	41.0
48-49	35.91148799603599	39.0	34.5	41.0	28.0	41.0
50-51	35.70233363752374	38.0	34.0	40.0	27.5	41.0
52-53	35.53587354206563	38.0	34.0	40.0	27.0	41.0
54-55	35.35028866671357	38.0	34.0	40.0	27.0	41.0
56-57	35.22794841128115	37.5	34.0	40.0	27.0	41.0
58-59	34.98326924688806	37.0	33.0	40.0	26.5	41.0
60-61	34.734637009728594	36.5	33.0	40.0	26.0	41.0
62-63	34.47771404104113	36.0	33.0	40.0	26.0	41.0
64-65	34.20406250725833	35.5	33.0	39.0	26.0	41.0
66-67	33.91571591734953	35.0	32.0	39.0	26.0	41.0
68-69	33.627989041066385	35.0	32.0	38.5	26.0	40.5
70-71	33.54533789203788	35.0	32.0	38.0	26.0	40.0
72-73	33.33073419402052	35.0	32.0	37.0	26.0	40.0
74-75	33.05425692094898	35.0	32.0	37.0	25.0	39.5
76-77	32.81493301134705	35.0	32.0	36.0	25.0	39.0
78-79	32.5977201876847	35.0	32.0	36.0	25.0	39.0
80-81	32.36125315453086	35.0	31.5	36.0	24.5	38.0
82-83	32.12827348322482	35.0	31.0	35.0	24.0	37.0
84-85	31.937770283166376	35.0	31.0	35.0	24.0	37.0
86-87	31.76642316847021	35.0	31.0	35.0	24.0	36.0
88-89	31.750391739126485	35.0	32.0	35.0	24.0	36.0
90-91	31.684841959560742	35.0	32.0	35.0	24.0	36.0
92-93	31.549059876589133	35.0	31.0	35.0	24.0	36.0
94-95	31.52965048622778	35.0	31.0	35.0	24.0	35.0
96-97	31.45626474596959	35.0	31.0	35.0	24.0	35.0
98-99	31.384959977810148	35.0	31.0	35.0	23.5	35.0
100	30.919504552252697	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.753908418078094
1101	2	0.9222823930888246
1101	3	0.8937634469072364
1101	4	1.0070210527055465
1101	5	0.9513248254281024
1101	6	0.6455952429626919
1101	7	0.6511895004385124
1101	8	0.7982282484574341
1101	9	0.8976900893541213
1101	10-11	0.968611593698121
1101	12-13	0.8218381659184146
1101	14-15	1.0372999540660217
1101	16-17	1.2234143614305921
1101	18-19	1.2998119034854696
1101	20-21	0.949903522397122
1101	22-23	1.294491842752393
1101	24-25	1.1179094498073496
1101	26-27	1.0475029269989236
1101	28-29	1.1683543683029356
1101	30-31	1.213710089674926
1101	32-33	1.0808970996880092
1101	34-35	0.987578268428841
1101	36-37	0.892764540683757
1101	38-39	0.9284101533320097
1101	40-41	0.864839113629678
1101	42-43	0.9018114255321663
1101	44-45	0.931915804278745
1101	46-47	0.9443555111504054
1101	48-49	0.8957245030223291
1101	50-51	0.9168221788188546
1101	52-53	0.9772499164743778
1101	54-55	0.9517774100888872
1101	56-57	0.8518648559183646
1101	58-59	0.8245822750909966
1101	60-61	0.8212200109828061
1101	62-63	0.7651962724905843
1101	64-65	0.7797045925763868
1101	66-67	0.8135247037587234
1101	68-69	0.8570469593562322
1101	70-71	0.6838321355458419
1101	72-73	0.6896068203390158
1101	74-75	0.6796182549399887
1101	76-77	0.624257226219683
1101	78-79	0.5957325502376847
1101	80-81	0.5825960803400179
1101	82-83	0.6000828249485082
1101	84-85	0.6578177192441075
1101	86-87	0.6006534386115732
1101	88-89	0.5564931610910975
1101	90-91	0.5589641975836415
1101	92-93	0.6231043000074159
1101	94-95	0.6506750589665877
1101	96-97	0.5949375649610573
1101	98-99	0.6419441088543394
1101	100	0.6661729711564846
1102	1	0.7363511417840485
1102	2	0.9257648072554581
1102	3	0.8274762827795676
1102	4	0.8964431252908511
1102	5	0.7380826198152377
1102	6	0.5138549748746897
1102	7	0.5912853263623319
1102	8	0.6430629029991266
1102	9	0.8167106494793472
1102	10-11	0.7749364531634342
1102	12-13	0.8356915413161516
1102	14-15	1.0192922020304849
1102	16-17	1.1930294653154192
1102	18-19	1.1669463656783208
1102	20-21	0.9232694527430283
1102	22-23	1.2496743708569085
1102	24-25	1.1062396499295204
1102	26-27	0.9744700253775633
1102	28-29	1.1112054194452696
1102	30-31	1.1595352390671465
1102	32-33	0.9942076486711358
1102	34-35	0.8419614646720817
1102	36-37	0.8337326642129455
1102	38-39	0.9320478163372385
1102	40-41	0.8398816346288029
1102	42-43	0.8155663516274245
1102	44-45	0.8828969091761394
1102	46-47	0.8467096546850428
1102	48-49	0.8566134847351563
1102	50-51	0.9544889109893191
1102	52-53	0.823841847503715
1102	54-55	0.8640225624045783
1102	56-57	0.7952284581367195
1102	58-59	0.7975828926982231
1102	60-61	0.8520728558965942
1102	62-63	0.8286980086638778
1102	64-65	0.7159668733282416
1102	66-67	0.8535045105192935
1102	68-69	0.8499496310399053
1102	70-71	0.7593162989542606
1102	72-73	0.7200705630194548
1102	74-75	0.7449295415668544
1102	76-77	0.7445098546805298
1102	78-79	0.6770217133605314
1102	80-81	0.7964216488920002
1102	82-83	0.6978588097077463
1102	84-85	0.7374818154514386
1102	86-87	0.8323566067043231
1102	88-89	0.8280895934897217
1102	90-91	0.8256746598307032
1102	92-93	0.9009738987272904
1102	94-95	0.817189905256928
1102	96-97	0.8817016757857346
1102	98-99	0.9011486588190607
1102	100	0.9408156447181497
1103	1	0.5654954283387603
1103	2	0.7983916385185807
1103	3	0.6867467907742295
1103	4	0.6892912147507104
1103	5	0.6230836003152405
1103	6	0.49261017032899446
1103	7	0.5860578264460372
1103	8	0.568866554285492
1103	9	0.6325732264246824
1103	10-11	0.6956216552660095
1103	12-13	0.6405921281812823
1103	14-15	0.7715153064369957
1103	16-17	0.9838702799435239
1103	18-19	1.0909216235976587
1103	20-21	0.8097567277661
1103	22-23	0.9896701618812642
1103	24-25	0.8953411532034323
1103	26-27	0.7882588448077215
1103	28-29	0.9903281828290957
1103	30-31	0.9711206836641111
1103	32-33	0.839674390589181
1103	34-35	0.8115113195102523
1103	36-37	0.8341466265330553
1103	38-39	0.8519054525191194
1103	40-41	0.8267183024593194
1103	42-43	0.8098875825614797
1103	44-45	0.8940604996236772
1103	46-47	0.8656905803727
1103	48-49	0.911731814345778
1103	50-51	0.8824228779010284
1103	52-53	0.8965559671821097
1103	54-55	0.8833502502899009
1103	56-57	0.7282436869758513
1103	58-59	0.8387845791582365
1103	60-61	0.7709700803641368
1103	62-63	0.7641160603864563
1103	64-65	0.7196728046358416
1103	66-67	0.7054332257773055
1103	68-69	0.7066149983447758
1103	70-71	0.6226617410641069
1103	72-73	0.6098356973957806
1103	74-75	0.597840670002789
1103	76-77	0.5524550136411079
1103	78-79	0.5443775200728922
1103	80-81	0.6690981030213479
1103	82-83	0.6438339767514591
1103	84-85	0.6420027574967122
1103	86-87	0.7124169433267156
1103	88-89	0.7138617013734176
1103	90-91	0.7207634309533049
1103	92-93	0.7905986542001564
1103	94-95	0.7065823300573619
1103	96-97	0.6505910084239446
1103	98-99	0.6936544102886089
1103	100	0.7497630660031298
1104	1	0.34099107683362817
1104	2	0.5636880885128477
1104	3	0.5290608286783893
1104	4	0.6674724002739012
1104	5	0.5316049189339935
1104	6	0.21870733878621706
1104	7	0.2379940584103224
1104	8	0.24860536512451858
1104	9	0.40851381187115976
1104	10-11	0.3522805603316357
1104	12-13	0.26779795674660534
1104	14-15	0.4831681617044694
1104	16-17	0.5947122447835227
1104	18-19	0.6305092982213196
1104	20-21	0.5151020753527078
1104	22-23	0.7364892976300368
1104	24-25	0.60619087799693
1104	26-27	0.3827206599053383
1104	28-29	0.5316464753625354
1104	30-31	0.5312313593830638
1104	32-33	0.3465653528629602
1104	34-35	0.31246045573351466
1104	36-37	0.3164369545665551
1104	38-39	0.48193825191494
1104	40-41	0.3755177585706022
1104	42-43	0.38144797081617554
1104	44-45	0.38911449308356794
1104	46-47	0.4088094292503257
1104	48-49	0.3186177845475555
1104	50-51	0.437532634287912
1104	52-53	0.49603820034845114
1104	54-55	0.45627846627164814
1104	56-57	0.3221049459269878
1104	58-59	0.3920802363521645
1104	60-61	0.26723294607047876
1104	62-63	0.28384458063745654
1104	64-65	0.29027194718569405
1104	66-67	0.4067547903420987
1104	68-69	0.3356935458791881
1104	70-71	0.3506061244394232
1104	72-73	0.38008289886987967
1104	74-75	0.27827515820172977
1104	76-77	0.34674222978496516
1104	78-79	0.3262234301325577
1104	80-81	0.35596084773172976
1104	82-83	0.31137779824683065
1104	84-85	0.2805840847749792
1104	86-87	0.21989217572990682
1104	88-89	0.18060691491547942
1104	90-91	0.23718000799333083
1104	92-93	0.2624326425987711
1104	94-95	0.26711935838070033
1104	96-97	0.30930412640292104
1104	98-99	0.3373661047856693
1104	100	0.39901445290946924
1105	1	0.3903375061607868
1105	2	0.601891995509277
1105	3	0.6197692082335067
1105	4	0.7223566267296491
1105	5	0.5929559202100307
1105	6	0.24192376574084307
1105	7	0.3372351661941764
1105	8	0.40384322829948616
1105	9	0.29577166850561554
1105	10-11	0.49743532849599603
1105	12-13	0.47144064739944014
1105	14-15	0.628702856897192
1105	16-17	0.7605814520986343
1105	18-19	0.7716233340402638
1105	20-21	0.54191799028753
1105	22-23	0.7558413296785602
1105	24-25	0.6607671150842194
1105	26-27	0.5143293062109038
1105	28-29	0.7549125172603155
1105	30-31	0.7680381127863782
1105	32-33	0.5745766753296238
1105	34-35	0.48507316741859796
1105	36-37	0.44029897693255293
1105	38-39	0.5554256774524475
1105	40-41	0.4717364827211341
1105	42-43	0.4480087415069747
1105	44-45	0.489877312815409
1105	46-47	0.44320524909117154
1105	48-49	0.43961752965980594
1105	50-51	0.4857412863645365
1105	52-53	0.5021416799670533
1105	54-55	0.4141712899790946
1105	56-57	0.3961580539545082
1105	58-59	0.37755644362239593
1105	60-61	0.42421174069187373
1105	62-63	0.34451267510302586
1105	64-65	0.36861245959023137
1105	66-67	0.31099634698488643
1105	68-69	0.39132740683550793
1105	70-71	0.36748041508199947
1105	72-73	0.3402839395813828
1105	74-75	0.32341513806720457
1105	76-77	0.3337415516873605
1105	78-79	0.3227058989849567
1105	80-81	0.4803673119416416
1105	82-83	0.4794325878922976
1105	84-85	0.4218437993648152
1105	86-87	0.3631016213250007
1105	88-89	0.3589107170712822
1105	90-91	0.3609558654742102
1105	92-93	0.3950075379184561
1105	94-95	0.27882856511871523
1105	96-97	0.34323165011229406
1105	98-99	0.3995344701696659
1105	100	0.40930614709546376
1106	1	0.4576804265156049
1106	2	0.6182827796518602
1106	3	0.576780533718658
1106	4	0.7551833829949288
1106	5	0.5817942061082491
1106	6	0.2826926161095713
1106	7	0.3727550411696967
1106	8	0.3307720475139817
1106	9	0.5337928915734835
1106	10-11	0.39414402564445083
1106	12-13	0.3347618658258753
1106	14-15	0.6147372003281077
1106	16-17	0.6836403783567562
1106	18-19	0.6247358189037087
1106	20-21	0.44668946363454154
1106	22-23	0.8228099869559458
1106	24-25	0.6723250258863587
1106	26-27	0.501633299809491
1106	28-29	0.6867267325875446
1106	30-31	0.6298104400824442
1106	32-33	0.57963968661889
1106	34-35	0.5336635241338357
1106	36-37	0.5572721199895767
1106	38-39	0.6659963488118663
1106	40-41	0.5399950492867092
1106	42-43	0.5217315631538781
1106	44-45	0.5952593852059849
1106	46-47	0.6427965242827725
1106	48-49	0.64855276405806
1106	50-51	0.5977173369237505
1106	52-53	0.4994190098272284
1106	54-55	0.5906308240014013
1106	56-57	0.5268374943858092
1106	58-59	0.5168112230767505
1106	60-61	0.4314828074414052
1106	62-63	0.45061157602569324
1106	64-65	0.5514596542185402
1106	66-67	0.5975391778316563
1106	68-69	0.4624908105337937
1106	70-71	0.46771744915052693
1106	72-73	0.44206212905090325
1106	74-75	0.4469833441817457
1106	76-77	0.4314619737487533
1106	78-79	0.5382515735613111
1106	80-81	0.5164763523300664
1106	82-83	0.5167904297143693
1106	84-85	0.4724858696195824
1106	86-87	0.40841523628415644
1106	88-89	0.4087381537033963
1106	90-91	0.4216637469749145
1106	92-93	0.5295710220305914
1106	94-95	0.5771260961757783
1106	96-97	0.5431288403821775
1106	98-99	0.4655609250433841
1106	100	0.48941259639190804
1107	1	0.4754550323550788
1107	2	0.6529352803014241
1107	3	0.5017672463691767
1107	4	0.6253452980461986
1107	5	0.5809781814907495
1107	6	0.35039374364665576
1107	7	0.35567792864767966
1107	8	0.3760692325945456
1107	9	0.5766126244348371
1107	10-11	0.5957242671707093
1107	12-13	0.4770902906124377
1107	14-15	0.6804379386828003
1107	16-17	0.7828050195354024
1107	18-19	0.7775174366884698
1107	20-21	0.5812939456907031
1107	22-23	0.8813240597025143
1107	24-25	0.6199158934059241
1107	26-27	0.5320578371025348
1107	28-29	0.6920293889840892
1107	30-31	0.719615804470429
1107	32-33	0.6456787851073926
1107	34-35	0.5285476863905032
1107	36-37	0.4559426401726583
1107	38-39	0.5326967520218489
1107	40-41	0.3654853996730054
1107	42-43	0.47483004165983544
1107	44-45	0.47841750318433185
1107	46-47	0.4470976467121801
1107	48-49	0.4489932452451697
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2216	90-91	-1.1767465752928565
2216	92-93	-1.1819416538966827
2216	94-95	-1.098984407446327
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2216	98-99	-0.9527207142365128
2216	100	-1.0790865076476166
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	12106.0
1	2092.0
2	305.0
3	334.0
4	295.0
5	155.0
6	159.0
7	261.0
8	487.0
9	902.0
10	1353.0
11	2208.0
12	3508.0
13	4672.0
14	6056.0
15	7924.0
16	9355.0
17	11173.0
18	13016.0
19	15172.0
20	17405.0
21	20229.0
22	23092.0
23	27147.0
24	30669.0
25	33839.0
26	38091.0
27	43739.0
28	52164.0
29	62213.0
30	73720.0
31	88152.0
32	109080.0
33	139075.0
34	190883.0
35	258569.0
36	380391.0
37	583921.0
38	527927.0
39	178681.0
40	207.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.388682578659505	20.950401449256635	12.925462429966537	23.73545354211732
2	15.295741035596583	19.84261723487685	41.010507850710546	23.851133878816015
3	18.4009757391102	26.546171725753993	29.362380467900184	25.69047206723562
4	12.238299794988965	16.96779142037949	39.89155585857417	30.902352926057368
5	13.172751617633088	38.400390010247236	35.077696086137635	13.349162285982047
6	29.84244846544711	38.367272523090854	18.26309732962698	13.527181681835062
7	26.759676610123844	32.140260564521505	23.569589821818905	17.530473003535747
8	25.84271829747441	35.41736094594078	20.215021603997425	18.524899152587388
9	25.88772077760141	15.468009432567262	21.630410250354196	37.01385953947713
10-11	25.402035954507866	26.163932562158443	27.77075655130065	20.663274932033044
12-13	25.609734224575764	23.94569363676727	27.726468844417013	22.718103294239953
14-15	23.06637791866247	25.204966470184242	25.095933021923607	26.63272258922968
16-17	22.18783678164459	27.85899220633656	26.420652742791166	23.53251826922768
18-19	21.777462824005028	28.194448533654427	27.951870957392916	22.076217684947636
20-21	23.35472932672418	27.301101441035808	26.95981461881883	22.384354613421184
22-23	23.620902014365466	26.983201273833206	26.776595784267	22.61930092753433
24-25	23.062705028706556	27.162297796941804	27.357808975342074	22.41718819900957
26-27	22.92976606280808	27.38311387219179	26.594430769298434	23.092689295701692
28-29	22.249683427325316	27.82396782927999	27.050862253456074	22.875486489938616
30-31	22.885565826562267	27.406635939929075	26.991058590417254	22.7167396430914
32-33	23.22038999685822	27.69543788911667	26.15310668194076	22.931065432084353
34-35	23.29784218919868	27.470044036091828	26.96577367733093	22.266340097378563
36-37	23.012392129041693	27.19089402583758	26.72965852258419	23.067055322536532
38-39	23.13735006305119	27.169461834569653	27.19748458579463	22.495703516584527
40-41	22.816885248481654	26.49721308820491	27.75267297984221	22.933228683471228
42-43	22.68625771360244	26.782348563280735	27.15264209392344	23.37875162919339
44-45	22.880208692084906	27.559939825355436	26.912532677949386	22.647318804610276
46-47	22.838535985771916	27.21726988055429	26.66931465995992	23.274879473713874
48-49	23.43597729339917	26.865806235923422	26.835047562586624	22.863168908090785
50-51	23.10532791413851	26.911805193269068	26.66796511648567	23.31490177610675
52-53	22.63916358450152	27.418559859710793	26.55882486810239	23.383451687685287
54-55	22.6671691349547	27.296313356101216	26.59307209720494	23.443445411739145
56-57	23.500125376595083	26.78243726009671	26.47446090602102	23.24297645728719
58-59	22.702840266283214	27.086797213711872	27.12150229161809	23.088860228386825
60-61	22.69473378586834	27.6003716248741	26.61610037187867	23.088794217378894
62-63	23.03566748739261	27.03534873915956	26.243553765331935	23.685430008115897
64-65	22.31805168259469	27.199809811300323	26.610800146194872	23.871338359910116
66-67	22.966519320657348	26.83488081893361	26.55615574966689	23.642444110742158
68-69	22.501921696332378	26.9491044099673	26.73061828358247	23.81835561011785
70-71	22.499471221773817	26.62128480418285	26.98692860224879	23.892315371794545
72-73	23.0422115649667	26.727899351376212	27.197035191449014	23.03285389220807
74-75	23.118726069296752	27.082973759393763	26.82064553421012	22.977654637099366
76-77	22.816664684314507	26.98493372881118	26.62166417082044	23.576737416053874
78-79	22.680439415076787	26.951381469240214	26.72485006362681	23.64332905205619
80-81	22.440159433843025	27.16862462168979	27.172448543297367	23.218767401169814
82-83	23.0980780915225	26.347297790487968	27.961908010515344	22.59271610747419
84-85	23.342366004936327	26.875379252065006	26.783580369625447	22.998674373373216
86-87	22.27378714044757	27.38686153192788	27.179287322323248	23.160064005301297
88-89	23.05923541240636	26.36931097251192	27.035216847035677	23.53623676804605
90-91	22.87956958650952	26.346029219758123	27.27432699389928	23.500074199833083
92-93	23.083441087564964	26.52923931922697	27.175645580873876	23.211674012334186
94-95	22.10766429413756	26.510030163075992	27.727990085311998	23.654315457474453
96-97	22.488397608029327	26.47213936859095	27.287910350962253	23.751552672417475
98-99	21.934938876183534	27.09094470360943	27.19776269320876	23.776353726998266
100	22.675439387602093	26.468961672720514	26.539000956659027	24.31659798301837
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10412.0
1	6112.5
2	1767.0
3	1385.0
4	800.5
5	495.5
6	409.0
7	370.0
8	387.5
9	408.5
10	379.0
11	340.0
12	327.5
13	323.0
14	366.5
15	508.5
16	596.0
17	663.5
18	840.0
19	1028.0
20	1266.0
21	1699.0
22	2328.5
23	3126.0
24	4133.5
25	5557.0
26	7619.5
27	9946.5
28	13329.0
29	19476.5
30	27706.0
31	35886.5
32	45883.0
33	54273.5
34	60475.5
35	68232.5
36	76069.0
37	88584.5
38	94247.5
39	89652.5
40	84760.0
41	79979.0
42	79746.5
43	84796.0
44	92545.5
45	107357.5
46	120789.5
47	129504.0
48	134806.5
49	132437.0
50	127478.5
51	128305.5
52	127532.0
53	121140.0
54	115061.5
55	101921.0
56	90099.5
57	75718.5
58	61510.5
59	57041.0
60	47469.0
61	35161.5
62	27239.5
63	21831.0
64	15987.0
65	9741.5
66	6687.0
67	4924.5
68	3766.5
69	2945.5
70	2197.0
71	1711.5
72	1439.0
73	1190.5
74	958.5
75	787.0
76	572.5
77	374.0
78	273.0
79	189.0
80	115.5
81	84.5
82	65.0
83	52.0
84	43.5
85	32.5
86	27.0
87	24.0
88	22.5
89	18.0
90	14.0
91	9.0
92	4.5
93	6.5
94	6.5
95	4.5
96	3.0
97	3.5
98	3.0
99	1.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9974999385672262
2	0.44514356250170417
3	0.47796381155185247
4	0.4649703591073835
5	0.43275602234739174
6	0.4834170221632617
7	0.5020319941886279
8	0.5057347915173626
9	0.5195361270153737
10-11	0.5318058508910446
12-13	0.530206915680909
14-15	0.5274971412721532
16-17	0.532748381120177
18-19	0.5317216964063005
20-21	0.5296514960815989
22-23	0.49179880884376115
24-25	0.5269080598789454
26-27	0.5062902111166728
28-29	0.5131572170717807
30-31	0.5157155134079975
32-33	0.5182401479503165
34-35	0.5306613498985265
36-37	0.5477110485076548
38-39	0.5380837754529447
40-41	0.5423756541748872
42-43	0.5400698212929024
44-45	0.549579278068971
46-47	0.5483001299008626
48-49	0.5205123190384038
50-51	0.5359462515404478
52-53	0.5281030535623099
54-55	0.5379154664834568
56-57	0.5261338386193009
58-59	0.5327988738110234
60-61	0.5250061685237317
62-63	0.5189470456221659
64-65	0.5307455043832705
66-67	0.5272951705087677
68-69	0.5488892112940704
70-71	0.5319236671696861
72-73	0.5363165312733214
74-75	0.533236477131692
76-77	0.5212023858133042
78-79	0.5517336328784166
80-81	0.5266555964247135
82-83	0.5257130661955811
84-85	0.5351551993838545
86-87	0.8426725175352701
88-89	1.1108223677234563
90-91	1.1012455873595923
92-93	1.1012119255656947
94-95	1.1062443637533843
96-97	1.0989734162715052
98-99	1.0957418840573367
100	1.0898342392283102
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	2970727.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.530178236170514
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.57327453247143	31.03528464928284
2	12.804155596785199	10.379094170027289
3	4.470949683244732	5.436251626405773
4	2.0836791647047903	3.3780755172992025
5	1.1106012598508959	2.250643350553617
6	0.6481083485412253	1.5760768129635592
7	0.40132141207835603	1.1385939853069185
8	0.28219864377213716	0.9150049064072248
9	0.2024162196292115	0.73835689135174
>10	1.0848262243628828	8.370162371199301
>50	0.13347750174813017	3.839976005856295
>100	0.16696765737620362	14.470878387775235
>500	0.026483794724240412	7.283391574399481
>1k	0.010958811610029612	7.568362252896926
>5k	5.811491005318735E-4	1.6198474982747786
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	9117	0.30689457496431005	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	7432	0.25017445224687423	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	7423	0.24987149610179596	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	6505	0.21896996930381016	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	6183	0.2081308716687868	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	5944	0.20008570292726324	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	5536	0.18635169101704735	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	4901	0.16497645189207893	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	3929	0.13225718822362337	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	3709	0.12485159356615402	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	3585	0.1206775311228531	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	3507	0.11805191119884123	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	3383	0.11387784875554031	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	3219	0.10835731455633586	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	3056	0.10287044215102903	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	3009	0.10128833783784238	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	2980	0.10031214581481235	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.0098538169276409E-4
2	0.0	0.0	0.0	0.0	1.0098538169276409E-4
3	0.0	0.0	0.0	3.366179389758803E-5	1.0098538169276409E-4
4	0.0	0.0	0.0	3.366179389758803E-5	1.0098538169276409E-4
5	0.0	0.0	0.0	3.366179389758803E-5	1.0098538169276409E-4
6	0.0	0.0	0.0	3.366179389758803E-5	1.0098538169276409E-4
7	0.0	0.0	0.0	3.366179389758803E-5	1.0098538169276409E-4
8	0.0	0.0	0.0	3.366179389758803E-5	1.0098538169276409E-4
9	0.0	0.0	0.0	3.366179389758803E-5	1.0098538169276409E-4
10-11	0.0	0.0	0.0	3.366179389758803E-5	1.0098538169276409E-4
12-13	0.0	0.0	0.0	3.366179389758803E-5	1.3464717559035213E-4
14-15	0.0	0.0	0.0	3.366179389758803E-5	1.5147807253914616E-4
16-17	0.0	0.0	0.0	3.366179389758803E-5	1.6830896948794017E-4
18-19	0.0	0.0	0.0	5.0492690846382044E-5	1.6830896948794017E-4
20-21	0.0	0.0	0.0	6.732358779517606E-5	1.6830896948794017E-4
22-23	0.0	0.0	0.0	2.0197076338552818E-4	2.0197076338552818E-4
24-25	0.0	0.0	0.0	3.8711062982226235E-4	2.0197076338552818E-4
26-27	0.0	0.0	0.0	5.049269084638204E-4	2.0197076338552818E-4
28-29	0.0	0.0	0.0	0.0013801335498011093	2.0197076338552818E-4
30-31	0.0	0.0	0.0	0.005369056126665291	2.0197076338552818E-4
32-33	0.0	0.0	0.0	0.01516463815086341	2.0197076338552818E-4
34-35	0.0	0.0	0.0	0.029790687599365408	2.3563255728311622E-4
36-37	0.0	0.0	0.0	0.05608054863338166	2.3563255728311622E-4
38-39	0.0	0.0	0.0	0.09995869697888767	2.3563255728311622E-4
40-41	0.0	0.0	0.0	0.14959301208088122	2.3563255728311622E-4
42-43	0.0	0.0	0.0	0.20198759428247698	2.3563255728311622E-4
44-45	0.0	0.0	0.0	0.2622758671530572	2.3563255728311622E-4
46-47	0.0	0.0	0.0	0.34128009743069626	2.3563255728311622E-4
48-49	0.0	0.0	0.0	0.43735085721441247	2.3563255728311622E-4
50-51	0.0	0.0	0.0	0.5432171990223269	2.3563255728311622E-4
52-53	0.0	0.0	0.0	0.6487974155821117	2.6929435118070425E-4
54-55	0.0	0.0	0.0	0.7678591805978805	2.6929435118070425E-4
56-57	0.0	0.0	0.0	0.9109722973534762	2.6929435118070425E-4
58-59	0.0	0.0	0.0	1.087073972128708	2.6929435118070425E-4
60-61	0.0	0.0	0.0	1.2771621222683875	2.6929435118070425E-4
62-63	0.0	0.0	0.0	1.4745043889929974	3.0295614507829227E-4
64-65	0.0	0.0	0.0	1.6833926510244799	3.0295614507829227E-4
66-67	0.0	0.0	0.0	1.9215161810560175	3.0295614507829227E-4
68-69	0.0	0.0	0.0	2.1957588159396675	3.0295614507829227E-4
70-71	0.0	0.0	0.0	2.5036127520300586	3.0295614507829227E-4
72-73	0.0	0.0	0.0	2.8173238402586303	3.3661793897588033E-4
74-75	0.0	0.0	0.0	3.1329704816363133	3.7027973287346834E-4
76-77	0.0	0.0	0.0	3.497914820177014	3.7027973287346834E-4
78-79	0.0	0.0	0.0	3.8965546144092	3.7027973287346834E-4
80-81	0.0	0.0	0.0	4.329798732768108	3.7027973287346834E-4
82-83	0.0	0.0	0.0	4.786505121473633	3.7027973287346834E-4
84-85	0.0	0.0	0.0	5.228467644452014	3.7027973287346834E-4
86-87	0.0	0.0	0.0	5.706902721118434	4.0394152677105635E-4
88	0.0	0.0	0.0	6.091640194470916	4.376033206686444E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4110	0.0	35.12464	1
GTACTAG	2485	0.0	32.27415	1
GGGCACC	2510	0.0	31.411802	94
GTATCAA	6265	0.0	31.175364	1
CAACGCA	5805	0.0	31.001923	5
ATCAACG	6165	0.0	29.26781	3
TAGGCAT	3055	0.0	28.762224	5
GCATCGT	2565	0.0	27.273773	8
AACGCAG	7045	0.0	26.390392	6
CTAGGCA	3550	0.0	26.075338	4
GTACATG	11990	0.0	26.047802	1
TATCAAC	7470	0.0	25.854015	2
TACATGG	12090	0.0	25.341253	2
ACGCAGA	7135	0.0	25.07133	7
ACATGGG	11790	0.0	24.669987	3
TAGTACT	3640	0.0	24.65608	4
CGCAGAG	7220	0.0	24.646109	8
GCTATTG	3355	0.0	24.350231	8
CTGTGCG	3470	0.0	24.221407	9
TCTAACG	1255	0.0	23.963102	2
>>END_MODULE