FastQCFastQC Report
Mon 16 Jan 2017
ERR1146485_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146485_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2970727
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC82070.276262342517505No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA79860.26882308606613803No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67690.2278566828927734No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66760.2247261360602977No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC65850.22166291281561717No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC65690.22112432411325578No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA64700.21779180651739458No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT64300.21644533476149105No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC54490.18342311494795718No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC41120.138417296506882No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT39740.13377196894901483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39500.13296408589547273No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG37130.12498624074174436No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG34450.11596487997719077No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT33710.11347390722876927No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG32870.11064631654137186No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC32520.10946815375495628No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC31620.10643859230417335No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC31290.10532775310555295No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA31280.10529409131165536No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA30450.10250016241815556No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA30260.10186058833410137No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64700.047.887681
GTATCAA106900.036.416261
GTGGTAT20300.032.6562081
CAACGCA124500.030.0859365
GGGCACC24250.030.06695294
ATCAACG127850.029.3054983
GTACTAG27650.029.0766031
TATCAAC138550.027.9588622
AACGCAG138950.027.9037536
TAGGCAT34100.027.0132125
TGGTATC25800.025.8742312
CTGTGCG31650.025.3949939
TCAACGC149550.025.3046864
GCATCGT30550.024.9213188
ACGCAGA151250.024.7956077
TATTCTC18900.024.6177235
CTAGGCA38650.024.5693324
CGCAGAG152350.024.5240358
TAGTACT34600.023.9126684
CGACGAG15850.022.9794524-25
Produced by FastQC (version 0.11.5)