##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146485_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2970727 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.82264644310972 31.0 31.0 34.0 30.0 34.0 2 31.819258720171863 31.0 31.0 34.0 30.0 34.0 3 31.992139297889036 33.0 31.0 34.0 30.0 34.0 4 35.727881424311285 37.0 35.0 37.0 35.0 37.0 5 35.63386403395533 37.0 35.0 37.0 33.0 37.0 6 35.666272935884045 37.0 35.0 37.0 33.0 37.0 7 35.57897915224119 37.0 35.0 37.0 33.0 37.0 8 35.56562686507377 37.0 35.0 37.0 33.0 37.0 9 37.163931253191556 39.0 37.0 39.0 33.0 39.0 10-11 37.10489368427324 39.0 37.0 39.0 33.0 39.0 12-13 37.042978873521534 39.0 37.0 39.0 33.0 39.0 14-15 38.23116462737909 40.0 37.0 41.0 33.0 41.0 16-17 38.23356067386872 40.0 37.0 41.0 33.0 41.0 18-19 38.30267153461089 40.0 38.0 41.0 33.0 41.0 20-21 38.32255252670474 40.0 38.0 41.0 33.0 41.0 22-23 38.29353690190987 40.0 38.0 41.0 33.0 41.0 24-25 38.231087205253125 40.0 37.0 41.0 33.0 41.0 26-27 38.08670049452542 40.0 37.0 41.0 33.0 41.0 28-29 38.018081600901056 40.0 37.0 41.0 33.0 41.0 30-31 37.890477650756864 40.0 37.0 41.0 33.0 41.0 32-33 37.76145855879723 40.0 36.5 41.0 33.0 41.0 34-35 37.66331069802106 40.0 36.0 41.0 32.0 41.0 36-37 37.57626634827098 40.0 36.0 41.0 32.0 41.0 38-39 37.46367757791275 40.0 36.0 41.0 32.0 41.0 40-41 37.3389931151533 40.0 36.0 41.0 31.0 41.0 42-43 37.20911682561204 39.5 35.5 41.0 31.0 41.0 44-45 37.017993575310015 39.0 35.0 41.0 31.0 41.0 46-47 36.78213127628355 39.0 35.0 41.0 30.0 41.0 48-49 36.532356389530236 39.0 35.0 41.0 30.0 41.0 50-51 36.3394465395171 39.0 35.0 41.0 29.5 41.0 52-53 36.16112672083298 38.5 35.0 41.0 29.0 41.0 54-55 35.97411912976184 38.0 35.0 40.0 29.0 41.0 56-57 35.73387608487754 38.0 34.5 40.0 28.0 41.0 58-59 35.47669122743355 37.5 34.0 40.0 28.0 41.0 60-61 35.22680222719893 37.0 34.0 40.0 28.0 41.0 62-63 34.9619507278858 36.5 34.0 40.0 27.5 41.0 64-65 34.6971707598847 36.0 34.0 39.0 27.0 41.0 66-67 34.472698433750395 35.5 33.0 39.0 27.0 41.0 68-69 34.28275149483611 35.0 33.0 39.0 27.0 41.0 70-71 34.05444576361275 35.0 33.0 38.5 26.0 40.5 72-73 33.83861374673607 35.0 33.0 37.5 26.0 40.0 74-75 33.64330583052566 35.0 33.0 37.0 26.5 39.5 76-77 33.46701901588399 35.0 33.0 36.5 27.0 39.0 78-79 33.337249265920434 35.0 33.0 36.0 27.0 39.0 80-81 33.17013983445803 35.0 33.0 36.0 27.0 38.5 82-83 32.97020207511495 35.0 33.0 35.5 27.0 37.0 84-85 32.81356785729554 35.0 33.0 35.0 26.5 37.0 86-87 32.6644269231067 35.0 33.0 35.0 26.0 36.5 88-89 32.5421753665012 35.0 33.0 35.0 26.0 36.0 90-91 32.446810157917575 35.0 33.0 35.0 26.0 36.0 92-93 32.366076384669476 35.0 33.0 35.0 26.0 36.0 94-95 32.31507371764555 35.0 33.0 35.0 26.0 35.5 96-97 32.26493464394406 35.0 33.0 35.0 26.0 35.0 98-99 32.221760532017925 35.0 33.0 35.0 25.0 35.0 100 31.733443025899046 34.0 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.475968123575111 1101 2 0.6039472564417316 1101 3 0.49150367256970995 1101 4 0.36179322239644307 1101 5 0.3890446652883668 1101 6 0.3799083601166444 1101 7 0.439999409410639 1101 8 0.4638691905249175 1101 9 0.5003408813085599 1101 10-11 0.5122777001099976 1101 12-13 0.5218310851334991 1101 14-15 0.6633903567000203 1101 16-17 0.6486519748785611 1101 18-19 0.6471909893521541 1101 20-21 0.6267640181604932 1101 22-23 0.6486684632769268 1101 24-25 0.6840576828778211 1101 26-27 0.7358441077435245 1101 28-29 0.7729516529721607 1101 30-31 0.7441050543539376 1101 32-33 0.7140359243044401 1101 34-35 0.7382136901511771 1101 36-37 0.7564737690833212 1101 38-39 0.8080127660573311 1101 40-41 0.800639906043088 1101 42-43 0.7456731386413509 1101 44-45 0.6705975337225709 1101 46-47 0.7073500087165812 1101 48-49 0.756657027495585 1101 50-51 0.76446112380318 1101 52-53 0.7714436782225675 1101 54-55 0.7166184546335401 1101 56-57 0.6905518811987932 1101 58-59 0.6612964533133336 1101 60-61 0.715779746003065 1101 62-63 0.7004438428473065 1101 64-65 0.6199397405874194 1101 66-67 0.6152008728984057 1101 68-69 0.5726007349329194 1101 70-71 0.61828329164792 1101 72-73 0.525895712731959 1101 74-75 0.505587908886227 1101 76-77 0.5135177745408299 1101 78-79 0.455345789499809 1101 80-81 0.4578933099002924 1101 82-83 0.4480469763867845 1101 84-85 0.5174368872667898 1101 86-87 0.4892211495970642 1101 88-89 0.5394006559801667 1101 90-91 0.44935366204227734 1101 92-93 0.4751133147669506 1101 94-95 0.49818308489462737 1101 96-97 0.5387839496277351 1101 98-99 0.4784624461755129 1101 100 0.5264374852509341 1102 1 0.4320880497332915 1102 2 0.4892246811551182 1102 3 0.4435649608906438 1102 4 0.32527777592353146 1102 5 0.2910489846668156 1102 6 0.2998558635023514 1102 7 0.3579860098507055 1102 8 0.3411075085035762 1102 9 0.4240242307919715 1102 10-11 0.41755361952677816 1102 12-13 0.44230294963938377 1102 14-15 0.6132730064867218 1102 16-17 0.5661365032366987 1102 18-19 0.5659427983701875 1102 20-21 0.5493077709859548 1102 22-23 0.560649414256531 1102 24-25 0.6204668873551853 1102 26-27 0.5652515532987366 1102 28-29 0.606301741402298 1102 30-31 0.6058996825263208 1102 32-33 0.6142786836341401 1102 34-35 0.6970856712086331 1102 36-37 0.6068782388946232 1102 38-39 0.6362466610776636 1102 40-41 0.628835128008987 1102 42-43 0.6667983067166716 1102 44-45 0.6584481928101553 1102 46-47 0.6731122793040498 1102 48-49 0.7048536482740957 1102 50-51 0.676370754700578 1102 52-53 0.569570430973485 1102 54-55 0.6416828290545382 1102 56-57 0.6454515732664134 1102 58-59 0.6222076998635018 1102 60-61 0.630225269270305 1102 62-63 0.6015226710198718 1102 64-65 0.6172431783751122 1102 66-67 0.560154862128023 1102 68-69 0.5968556857131091 1102 70-71 0.5894884921330927 1102 72-73 0.6111430557140309 1102 74-75 0.5551800993493288 1102 76-77 0.5458392758878574 1102 78-79 0.5059525063172146 1102 80-81 0.5020534850761607 1102 82-83 0.5169910132341187 1102 84-85 0.4795280007893936 1102 86-87 0.5106668103254748 1102 88-89 0.519115888710644 1102 90-91 0.580669421965375 1102 92-93 0.4980308748505493 1102 94-95 0.4556410309730623 1102 96-97 0.4271481520286642 1102 98-99 0.5042565748411008 1102 100 0.5980637573717793 1103 1 0.266677459376627 1103 2 -0.2611199287254493 1103 3 0.09100776904265828 1103 4 0.1484560786541067 1103 5 0.11666251461032573 1103 6 0.1994375965480728 1103 7 0.21452977124005201 1103 8 0.2746556749685638 1103 9 0.3412138307957946 1103 10-11 0.3430500873352855 1103 12-13 0.358282349573372 1103 14-15 0.4660316791288537 1103 16-17 0.45969940111643126 1103 18-19 0.4529426835799413 1103 20-21 0.4225019769483467 1103 22-23 0.407844705943333 1103 24-25 0.37970104510567637 1103 26-27 0.38395925428928024 1103 28-29 0.4329200447068118 1103 30-31 0.5696667396392456 1103 32-33 0.5445898847705593 1103 34-35 0.488991404503075 1103 36-37 0.4445336242311271 1103 38-39 0.5025325270504837 1103 40-41 0.567185113899356 1103 42-43 0.5146007909688777 1103 44-45 0.5121795932033066 1103 46-47 0.5750717248671648 1103 48-49 0.5622747180235166 1103 50-51 0.4197240408104861 1103 52-53 0.48381159571184185 1103 54-55 0.5117497182867368 1103 56-57 0.4944656924665196 1103 58-59 0.5048130318703627 1103 60-61 0.4810056037882049 1103 62-63 0.49548111695127517 1103 64-65 0.4460952280984145 1103 66-67 0.5404402354593856 1103 68-69 0.3699489719186744 1103 70-71 0.4170990136156121 1103 72-73 0.3708006220651896 1103 74-75 0.47294711819884583 1103 76-77 0.46659729805193706 1103 78-79 0.4194585351965259 1103 80-81 0.3898240193769169 1103 82-83 0.3645643952895341 1103 84-85 0.3627370577397002 1103 86-87 0.3747536060992829 1103 88-89 0.33378823430580695 1103 90-91 0.32872097713404713 1103 92-93 0.27219721069810277 1103 94-95 0.296300453099839 1103 96-97 0.36514467962376074 1103 98-99 0.3491655079136038 1103 100 0.5628379793091476 1104 1 0.33111477872293094 1104 2 0.42946497406260065 1104 3 0.32824170643317885 1104 4 0.23795414311411633 1104 5 0.22818817089480348 1104 6 0.27435060598654815 1104 7 0.21320706986678317 1104 8 0.24716665752695377 1104 9 0.25378713022917054 1104 10-11 0.3177931439068331 1104 12-13 0.35305809680252764 1104 14-15 0.4199564362472543 1104 16-17 0.39387364321115825 1104 18-19 0.43401383395103466 1104 20-21 0.3913801150340035 1104 22-23 0.40501738912765006 1104 24-25 0.3996453118345542 1104 26-27 0.4911735891475857 1104 28-29 0.5322623021514303 1104 30-31 0.5154108005563671 1104 32-33 0.49038959987646535 1104 34-35 0.4961057002156224 1104 36-37 0.47309712338692833 1104 38-39 0.5005826533845052 1104 40-41 0.4734277493574979 1104 42-43 0.45062523828226375 1104 44-45 0.47940199445913834 1104 46-47 0.5298412295350019 1104 48-49 0.5125675944850627 1104 50-51 0.45909258324407887 1104 52-53 0.4493405230391261 1104 54-55 0.42085839618836474 1104 56-57 0.5358459657750387 1104 58-59 0.6005969057973175 1104 60-61 0.5395018879072921 1104 62-63 0.4777817751139608 1104 64-65 0.41822876553005983 1104 66-67 0.3935168346029272 1104 68-69 0.401222202352713 1104 70-71 0.37742803070134556 1104 72-73 0.42656641579475263 1104 74-75 0.4436390836319575 1104 76-77 0.3689595366354155 1104 78-79 0.35102535877571484 1104 80-81 0.3437431418793935 1104 82-83 0.30213734873772324 1104 84-85 0.26978376277447325 1104 86-87 0.30753535666570286 1104 88-89 0.3800504574682222 1104 90-91 0.4340101515540695 1104 92-93 0.32981125730132277 1104 94-95 0.33279684892270467 1104 96-97 0.3143655704071122 1104 98-99 0.27323782277199626 1104 100 0.38331886906442847 1105 1 0.10654704590942288 1105 2 -0.3505408450728993 1105 3 0.01090965982880121 1105 4 0.13635652591383973 1105 5 0.1761638170913642 1105 6 0.24477721434430322 1105 7 0.23843735053186776 1105 8 0.23812054688963968 1105 9 0.25316513998864565 1105 10-11 0.3306271029708938 1105 12-13 0.29215334947805616 1105 14-15 0.3391994997736205 1105 16-17 0.3504128766883454 1105 18-19 0.3864688518733317 1105 20-21 0.36592259953233963 1105 22-23 0.393707947379923 1105 24-25 0.40496509176542617 1105 26-27 0.3569174766507075 1105 28-29 0.41895648013041153 1105 30-31 0.3739923822711333 1105 32-33 0.37876459426573206 1105 34-35 0.4790835265841551 1105 36-37 0.4136449557188726 1105 38-39 0.44421318476936733 1105 40-41 0.4479536020291732 1105 42-43 0.4019166294884684 1105 44-45 0.38316062697833075 1105 46-47 0.43592030244892754 1105 48-49 0.4886852933135373 1105 50-51 0.5234102615532947 1105 52-53 0.4803805211795691 1105 54-55 0.42052395620400773 1105 56-57 0.4099121788804396 1105 58-59 0.40330737254648596 1105 60-61 0.4262766200346917 1105 62-63 0.3858616768138532 1105 64-65 0.3387933579340867 1105 66-67 0.36511648769909044 1105 68-69 0.4562545121808057 1105 70-71 0.3624339380055659 1105 72-73 0.3686119140602315 1105 74-75 0.3571870490185205 1105 76-77 0.3811676981733285 1105 78-79 0.26241759896654315 1105 80-81 0.25805717320975674 1105 82-83 0.23032152197162503 1105 84-85 0.3150157186452347 1105 86-87 0.3337022295937473 1105 88-89 0.3190629517773331 1105 90-91 0.3289599274452186 1105 92-93 0.2882657558297055 1105 94-95 0.29585535768507754 1105 96-97 0.2812706399481826 1105 98-99 0.2517655333990447 1105 100 0.32459970407029815 1106 1 0.27402016236309024 1106 2 0.28004741752379303 1106 3 0.25306563024586026 1106 4 0.17170880914266462 1106 5 0.1810203457262034 1106 6 0.18926868636303595 1106 7 0.17133452080206268 1106 8 0.13661466452781923 1106 9 0.26151085992279377 1106 10-11 0.2952222733852352 1106 12-13 0.32109146307704606 1106 14-15 0.3697547817484974 1106 16-17 0.3464486927379866 1106 18-19 0.3624427849181373 1106 20-21 0.3241791416009292 1106 22-23 0.3309672907259369 1106 24-25 0.3321190490060886 1106 26-27 0.29781464107193756 1106 28-29 0.29781658550236756 1106 30-31 0.3269920952944432 1106 32-33 0.36248248768700364 1106 34-35 0.2848300461663129 1106 36-37 0.3683979906719159 1106 38-39 0.3022750977737303 1106 40-41 0.316993246132796 1106 42-43 0.3519660815535488 1106 44-45 0.3086351762400241 1106 46-47 0.3524622068949341 1106 48-49 0.28968259392134144 1106 50-51 0.3385861163796946 1106 52-53 0.3726879656681632 1106 54-55 0.36417940342161614 1106 56-57 0.38877455780212244 1106 58-59 0.2954623665635623 1106 60-61 0.3852758628765969 1106 62-63 0.36351716403084566 1106 64-65 0.2978519158960111 1106 66-67 0.23484013065404952 1106 68-69 0.2452488852867276 1106 70-71 0.2494015594499146 1106 72-73 0.2641311956487087 1106 74-75 0.24440865238238985 1106 76-77 0.2164990430468663 1106 78-79 0.24853889859926426 1106 80-81 0.31953233057524244 1106 82-83 0.27241334715470344 1106 84-85 0.2424716051767959 1106 86-87 0.23657658606595788 1106 88-89 0.2730374846241901 1106 90-91 0.21865356803417768 1106 92-93 0.2006751062888128 1106 94-95 0.18353560961325144 1106 96-97 0.2197948929691762 1106 98-99 0.2559642656073393 1106 100 0.2740827498043039 1107 1 0.06141720391067196 1107 2 -0.5544907989659933 1107 3 -0.056491906568240324 1107 4 0.007410362689199701 1107 5 0.08718561111417245 1107 6 0.11743589636756013 1107 7 0.1915448477004702 1107 8 0.20893897541774464 1107 9 0.16854156110251495 1107 10-11 0.22519449486722465 1107 12-13 0.2062496463817709 1107 14-15 0.2164361875648808 1107 16-17 0.2992175783550053 1107 18-19 0.28229219279831597 1107 20-21 0.25161499220227057 1107 22-23 0.18589828071119996 1107 24-25 0.25595836294211693 1107 26-27 0.3017671186259463 1107 28-29 0.22714014652682835 1107 30-31 0.2723680421568915 1107 32-33 0.306526831162401 1107 34-35 0.3220987944683813 1107 36-37 0.3785861075250878 1107 38-39 0.34731460913237555 1107 40-41 0.4472902350491452 1107 42-43 0.4434588745544161 1107 44-45 0.40807208779364856 1107 46-47 0.4179825111863451 1107 48-49 0.3923750448932424 1107 50-51 0.4340305541619571 1107 52-53 0.5000345569263516 1107 54-55 0.4684501897958313 1107 56-57 0.4329952223694633 1107 58-59 0.41581077641204445 1107 60-61 0.4090254039263641 1107 62-63 0.3650818512155425 1107 64-65 0.3568408078198999 1107 66-67 0.44933121591589753 1107 68-69 0.3717823666315141 1107 70-71 0.4272207167728723 1107 72-73 0.5052045904887734 1107 74-75 0.4820126009089023 1107 76-77 0.35404739937582264 1107 78-79 0.2485213178443786 1107 80-81 0.2668317970826095 1107 82-83 0.22872054475276826 1107 84-85 0.2504383936503345 1107 86-87 0.2535794892963068 1107 88-89 0.23497316089845555 1107 90-91 0.27148294995405564 1107 92-93 0.2333410972993093 1107 94-95 0.2871331456056865 1107 96-97 0.2578617150127016 1107 98-99 0.23110600350381816 1107 100 0.308395756824023 1108 1 0.14260124100935556 1108 2 0.3141955426758365 1108 3 0.1632338769578432 1108 4 0.14610761931643879 1108 5 0.10919766870694048 1108 6 0.12595364581019197 1108 7 0.12868392650766936 1108 8 0.08095147905112299 1108 9 0.06302791374668004 1108 10-11 0.18696010294323173 1108 12-13 0.18070394822042601 1108 14-15 0.25214310896285497 1108 16-17 0.21582336357604248 1108 18-19 0.18097687944364083 1108 20-21 0.1298130569064071 1108 22-23 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-0.8641356669935121 2215 22-23 -0.8248327791814489 2215 24-25 -0.8216152227297968 2215 26-27 -0.832967790499211 2215 28-29 -0.8359344426529418 2215 30-31 -0.846909608751858 2215 32-33 -0.8678907811363104 2215 34-35 -0.9331332041896871 2215 36-37 -0.8720337277936849 2215 38-39 -0.879713595029969 2215 40-41 -0.8984038775085352 2215 42-43 -0.8355181507069105 2215 44-45 -0.9390923403540157 2215 46-47 -0.8895411492336578 2215 48-49 -0.8398513152866229 2215 50-51 -0.8422611311426849 2215 52-53 -0.9265261773706612 2215 54-55 -0.9410442894395032 2215 56-57 -0.9197499307758363 2215 58-59 -0.8880479467492748 2215 60-61 -0.8671875604535302 2215 62-63 -0.7655742840137592 2215 64-65 -0.8040997085893693 2215 66-67 -0.8191163338769272 2215 68-69 -0.815618312575566 2215 70-71 -0.7241173182351375 2215 72-73 -0.7793980380544028 2215 74-75 -0.7745933353845302 2215 76-77 -0.666197856768818 2215 78-79 -0.7021581835210924 2215 80-81 -0.7015558659506311 2215 82-83 -0.7008286418467193 2215 84-85 -0.6633632973045493 2215 86-87 -0.7255208408901552 2215 88-89 -0.6744407581381715 2215 90-91 -0.6896421559881247 2215 92-93 -0.6523958482699541 2215 94-95 -0.7586213687082797 2215 96-97 -0.7347444037367055 2215 98-99 -0.6309833969811578 2215 100 -0.7927768171000729 2216 1 -0.3844984293744389 2216 2 -0.16990012219723738 2216 3 -0.35209238896932504 2216 4 -0.29307077188212816 2216 5 -0.3778441801080419 2216 6 -0.35823120466648106 2216 7 -0.3825697886974524 2216 8 -0.43463058152753575 2216 9 -0.4472336439320159 2216 10-11 -0.533820797584923 2216 12-13 -0.5353141394816845 2216 14-15 -0.667727568465132 2216 16-17 -0.7377695538634228 2216 18-19 -0.7815392299752872 2216 20-21 -0.6553898149217261 2216 22-23 -0.5984930256557064 2216 24-25 -0.6707321165319158 2216 26-27 -0.6766782214471192 2216 28-29 -0.7467700182325601 2216 30-31 -0.8072457273826075 2216 32-33 -0.7626582515550311 2216 34-35 -0.6368464527269992 2216 36-37 -0.7442609946910252 2216 38-39 -0.7948025025966672 2216 40-41 -0.764900454103298 2216 42-43 -0.8136772278474567 2216 44-45 -0.7791607129471245 2216 46-47 -0.707992880447911 2216 48-49 -0.8045272379277577 2216 50-51 -0.645340115064009 2216 52-53 -0.7548582326691005 2216 54-55 -0.7794627007830002 2216 56-57 -0.71240110502157 2216 58-59 -0.7712726812272876 2216 60-61 -0.7603731215699696 2216 62-63 -0.7573875835814974 2216 64-65 -0.7008703622701091 2216 66-67 -0.661062360325424 2216 68-69 -0.7186001135405107 2216 70-71 -0.8294619032785775 2216 72-73 -0.811928915559534 2216 74-75 -0.8316413340091628 2216 76-77 -0.7113209028763237 2216 78-79 -0.6879781361473931 2216 80-81 -0.5243555878757107 2216 82-83 -0.5974834878275956 2216 84-85 -0.5851658108944733 2216 86-87 -0.571298925365987 2216 88-89 -0.5295080187044761 2216 90-91 -0.5817523598915457 2216 92-93 -0.49654085656580804 2216 94-95 -0.5363691664858621 2216 96-97 -0.6202595382169065 2216 98-99 -0.503423176977229 2216 100 -0.5091291372109765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 2.0 12 5.0 13 36.0 14 186.0 15 547.0 16 1267.0 17 2680.0 18 4655.0 19 7359.0 20 10752.0 21 15054.0 22 20912.0 23 24856.0 24 26715.0 25 27566.0 26 30187.0 27 36367.0 28 45206.0 29 55810.0 30 70145.0 31 86191.0 32 107603.0 33 136367.0 34 183918.0 35 253426.0 36 373071.0 37 628755.0 38 594587.0 39 225967.0 40 535.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.94430908692422 21.420667045301133 14.222325316135652 24.412698551638996 2 14.834147536921932 20.350556387413686 40.12318083398865 24.692115241675733 3 17.870000676613447 26.29259256391725 30.38909985838245 25.44830690108686 4 12.38802683450127 17.555459630399326 38.51200104515022 31.544512489949184 5 12.6943442973376 38.42481224521398 35.174390617478444 13.706452839969973 6 29.614198813960353 38.79262550884009 17.72529081265293 13.867884864546626 7 25.86976418840018 32.71921713228346 23.564463687073737 17.84655499224262 8 26.00185072542849 35.319839217807626 19.762435255747164 18.91587480101672 9 25.757937366846566 14.886827365826614 21.51022291849773 37.845012348829094 10-11 25.353945347384666 26.215939734617148 27.49350579841231 20.936609119585878 12-13 25.767867596046358 23.94509828738891 28.0253284802003 22.261705636364432 14-15 23.050207542914084 24.99688601467108 25.85361912424633 26.099287318168507 16-17 21.68599951087907 27.555467508551622 27.132645523154892 23.625887457414414 18-19 21.215210956779266 27.11457498450716 29.761250360601966 21.90896369811161 20-21 22.62970398481718 26.27495770394173 29.21572033233627 21.879617978904818 22-23 22.826216116230917 25.9772337022803 29.02840973546028 22.168140446028502 24-25 22.347795674257515 26.12981603493017 29.634294904917212 21.888093385895104 26-27 22.268193097198775 26.349928796758633 28.826027616523458 22.555850489519127 28-29 21.66353555880429 26.784150815608438 29.250062358473194 22.302251267114077 30-31 22.363650379183277 26.39621547183568 29.052585444572994 22.187548704408044 32-33 22.6947982766508 26.68629598074815 28.4035523964336 22.215353346167454 34-35 22.642575500750578 26.54747652543638 29.04827136762171 21.761676606191337 36-37 22.519874764661985 26.181116608830095 28.797008274405556 22.502000352102364 38-39 22.569727881424313 26.175107978619376 29.292762344032287 21.962401795924027 40-41 22.2513697476177 25.63635007977045 29.649075650732154 22.46320452187969 42-43 22.35959339583819 25.85236993606731 28.896851838916888 22.89118482917761 44-45 22.534770108461665 26.583728494742196 28.56024804702687 22.321253349769265 46-47 22.586205329537183 26.382851739658342 27.99244090756236 23.03850202324212 48-49 23.320156905304422 26.23222267815528 27.853401326080746 22.59421909045955 50-51 22.932635681434206 26.393253233972697 27.470211837035173 23.20389924755792 52-53 22.31739907436799 26.880945303960953 27.410781939908986 23.390873681762073 54-55 22.448426917255343 26.78242232542608 27.292434825798466 23.476715931520108 56-57 23.192986489654793 26.31571063412667 27.185835369734008 23.305467506484533 58-59 22.39553037647439 26.57874185382668 27.879588113319333 23.1461396563796 60-61 22.462462866808607 27.04933980762272 27.3394373427362 23.148759982832473 62-63 22.838197518654525 26.492420878794988 26.960084181414178 23.70929742113631 64-65 22.08124475927946 26.630148108526967 27.311361831632457 23.97724530056111 66-67 22.630689390172844 26.3094353671677 27.322419730927816 23.73745551173164 68-69 22.296778489285472 26.2454442356263 27.57485053120709 23.882926743881132 70-71 22.265812837431504 25.850663590394905 27.743267527755705 24.14025604441788 72-73 22.72317516890647 26.01551741375091 27.913554493563357 23.347752923779264 74-75 22.808342758657833 26.426775327193408 27.4553980179889 23.30948389615985 76-77 22.51845388615548 26.402337133177678 27.117265323929324 23.96194365673752 78-79 22.256273105739606 26.42048596004164 27.359467872660392 23.96377306155836 80-81 21.998889460971153 26.727264372753318 27.743496790831706 23.530349375443823 82-83 22.6352548870305 25.908103528195163 28.50245180462717 22.954189780147168 84-85 22.92246913397381 26.368795971236874 27.28938632191223 23.419348572877084 86-87 21.85792009404463 26.83919516028338 27.657002371146046 23.645882374525943 88-89 22.382423918735334 25.943787416998166 27.55173680853825 24.12205185572825 90-91 22.16637222122727 25.843411132461398 27.835378292817907 24.15483835349342 92-93 22.37400188051619 26.1243961795829 27.570972990387688 23.930628949513224 94-95 21.305122350486606 25.989123054700812 28.207145350553592 24.498609244258986 96-97 21.62736988771716 25.978862366370727 27.784369273527027 24.609398472385095 98-99 21.079547348057055 26.577429812995113 27.672805234911575 24.67021760403626 100 21.70294323848206 25.98016144806673 27.07197918242365 25.244916131027562 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 321.0 1 221.0 2 174.0 3 229.0 4 157.5 5 120.0 6 260.0 7 688.5 8 1226.0 9 1406.0 10 1083.0 11 680.0 12 607.5 13 675.5 14 766.0 15 1059.5 16 1388.5 17 1724.0 18 2247.0 19 2899.0 20 3622.0 21 4389.5 22 5374.5 23 6444.0 24 7581.0 25 8839.5 26 10618.0 27 12696.5 28 15483.0 29 20979.5 30 29163.0 31 36657.5 32 46320.5 33 54791.5 34 59616.0 35 66523.5 36 73861.0 37 86876.0 38 93204.0 39 88279.0 40 83753.0 41 79011.5 42 78698.5 43 83642.0 44 90646.5 45 105973.0 46 120380.0 47 129285.0 48 135358.5 49 132481.0 50 126239.5 51 126459.0 52 125817.0 53 120520.0 54 116047.5 55 102123.5 56 89719.5 57 75829.0 58 61289.5 59 57881.0 60 48107.5 61 34881.0 62 26558.5 63 21074.0 64 15442.0 65 9048.0 66 6057.5 67 4571.0 68 3511.5 69 2679.0 70 1975.0 71 1619.0 72 1305.5 73 1019.0 74 825.0 75 647.0 76 453.5 77 280.0 78 164.5 79 98.5 80 63.5 81 35.5 82 20.0 83 18.0 84 12.0 85 6.0 86 2.5 87 1.5 88 1.0 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.013599364734625564 2 0.004544342176174384 3 0.0016830896948794017 4 0.027569009202124597 5 0.0032651940080660395 6 0.0 7 2.6929435118070425E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.008651081031680125 16-17 0.0030800541416293047 18-19 0.0 20-21 3.366179389758803E-5 22-23 6.732358779517607E-4 24-25 0.0 26-27 1.8513986643673417E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 3.871106298222624E-4 36-37 0.0 38-39 0.0 40-41 5.217578054126145E-4 42-43 8.247139504909068E-4 44-45 0.0 46-47 0.0 48-49 6.059122901565846E-4 50-51 0.0 52-53 0.0 54-55 5.217578054126145E-4 56-57 1.1781627864155812E-4 58-59 2.3563255728311624E-4 60-61 6.732358779517606E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0013464717559035213 70-71 0.0010603465077740231 72-73 0.0 74-75 2.3563255728311624E-4 76-77 0.0026592817179094544 78-79 3.197870420270863E-4 80-81 0.003282024905014833 82-83 0.0019523840460601057 84-85 0.006311586355797756 86-87 0.007489749142213337 88-89 0.007927352462881981 90-91 0.005756166756487554 92-93 0.008954037176758417 94-95 0.0013464717559035213 96-97 0.004998776393791823 98-99 0.00188506045826493 100 0.08300998375145209 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 2970727.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.148246119444956 #Duplication Level Percentage of deduplicated Percentage of total 1 70.70033436567873 24.142924186462498 2 15.781402236955543 10.778144153950343 3 5.663070101245124 5.801517348269657 4 2.6338119951335592 3.5976024096866857 5 1.4340331972479308 2.448485928153845 6 0.7935745132547422 1.6259506675645006 7 0.522113574400044 1.248048398864109 8 0.37376935054986427 1.0210854219585495 9 0.2619805712452641 0.8051559322856456 >10 1.3965352670738178 9.023934812343786 >50 0.17212983785982944 4.150853348663944 >100 0.21524679882883427 15.944843590505373 >500 0.03535483030131937 8.23671529841275 >1k 0.01575702743234283 9.118586349311277 >5k 8.863327930692843E-4 2.056152153567035 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 8207 0.276262342517505 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 7986 0.26882308606613803 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6769 0.2278566828927734 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6676 0.2247261360602977 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 6585 0.22166291281561717 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 6569 0.22112432411325578 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 6470 0.21779180651739458 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 6430 0.21644533476149105 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 5449 0.18342311494795718 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 4112 0.138417296506882 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 3974 0.13377196894901483 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3950 0.13296408589547273 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 3713 0.12498624074174436 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 3445 0.11596487997719077 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 3371 0.11347390722876927 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 3287 0.11064631654137186 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 3252 0.10946815375495628 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 3162 0.10643859230417335 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 3129 0.10532775310555295 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 3128 0.10529409131165536 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 3045 0.10250016241815556 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 3026 0.10186058833410137 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.366179389758803E-5 0.0 4 0.0 0.0 0.0 3.366179389758803E-5 0.0 5 0.0 0.0 0.0 3.366179389758803E-5 0.0 6 0.0 0.0 0.0 3.366179389758803E-5 0.0 7 0.0 0.0 0.0 3.366179389758803E-5 0.0 8 0.0 0.0 0.0 3.366179389758803E-5 0.0 9 0.0 0.0 0.0 3.366179389758803E-5 0.0 10-11 0.0 0.0 0.0 3.366179389758803E-5 0.0 12-13 0.0 0.0 0.0 3.366179389758803E-5 8.415448474397008E-5 14-15 0.0 0.0 0.0 3.366179389758803E-5 1.0098538169276409E-4 16-17 0.0 0.0 0.0 3.366179389758803E-5 1.0098538169276409E-4 18-19 0.0 0.0 0.0 3.366179389758803E-5 1.0098538169276409E-4 20-21 0.0 0.0 0.0 3.366179389758803E-5 1.0098538169276409E-4 22-23 0.0 0.0 0.0 1.6830896948794017E-4 1.0098538169276409E-4 24-25 0.0 0.0 0.0 3.8711062982226235E-4 1.0098538169276409E-4 26-27 0.0 0.0 0.0 5.049269084638204E-4 1.0098538169276409E-4 28-29 0.0 0.0 0.0 0.0014137953436986973 1.0098538169276409E-4 30-31 0.0 0.0 0.0 0.005402717920562879 1.0098538169276409E-4 32-33 0.0 0.0 0.0 0.01533294712035135 1.0098538169276409E-4 34-35 0.0 0.0 0.0 0.029958996568853348 1.0098538169276409E-4 36-37 0.0 0.0 0.0 0.056652799129640656 1.0098538169276409E-4 38-39 0.0 0.0 0.0 0.10101904348666169 1.0098538169276409E-4 40-41 0.0 0.0 0.0 0.15152856522999253 1.0098538169276409E-4 42-43 0.0 0.0 0.0 0.204276596267513 1.0098538169276409E-4 44-45 0.0 0.0 0.0 0.26596183358484304 1.1781627864155811E-4 46-47 0.0 0.0 0.0 0.34661549176346396 1.3464717559035213E-4 48-49 0.0 0.0 0.0 0.44418420137562287 1.5147807253914616E-4 50-51 0.0 0.0 0.0 0.5514475076302872 1.6830896948794017E-4 52-53 0.0 0.0 0.0 0.6583068723581804 1.6830896948794017E-4 54-55 0.0 0.0 0.0 0.7793883450078045 1.6830896948794017E-4 56-57 0.0 0.0 0.0 0.9235786391681229 1.6830896948794017E-4 58-59 0.0 0.0 0.0 1.1021039631039808 1.6830896948794017E-4 60-61 0.0 0.0 0.0 1.294363298950055 1.6830896948794017E-4 62-63 0.0 0.0 0.0 1.4939945676597008 2.0197076338552818E-4 64-65 0.0 0.0 0.0 1.705205493470117 2.6929435118070425E-4 66-67 0.0 0.0 0.0 1.9454665474141515 2.6929435118070425E-4 68-69 0.0 0.0 0.0 2.2221328314584277 2.6929435118070425E-4 70-71 0.0 0.0 0.0 2.5332182997629875 2.6929435118070425E-4 72-73 0.0 0.0 0.0 2.850834156083679 3.3661793897588033E-4 74-75 0.0 0.0 0.0 3.171311264885666 4.0394152677105635E-4 76-77 0.0 0.0 0.0 3.5426513442669085 4.0394152677105635E-4 78-79 0.0 0.0 0.0 3.9476700484426877 4.0394152677105635E-4 80-81 0.0 0.0 0.0 4.389716725905814 4.0394152677105635E-4 82-83 0.0 0.0 0.0 4.855966233181306 4.0394152677105635E-4 84-85 0.0 0.0 0.0 5.308330250474043 4.0394152677105635E-4 86-87 0.0 0.0 0.0 5.796375769298223 4.2077242371985036E-4 88 0.0 0.0 0.0 6.187609968872939 4.376033206686444E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6470 0.0 47.88768 1 GTATCAA 10690 0.0 36.41626 1 GTGGTAT 2030 0.0 32.656208 1 CAACGCA 12450 0.0 30.085936 5 GGGCACC 2425 0.0 30.066952 94 ATCAACG 12785 0.0 29.305498 3 GTACTAG 2765 0.0 29.076603 1 TATCAAC 13855 0.0 27.958862 2 AACGCAG 13895 0.0 27.903753 6 TAGGCAT 3410 0.0 27.013212 5 TGGTATC 2580 0.0 25.874231 2 CTGTGCG 3165 0.0 25.394993 9 TCAACGC 14955 0.0 25.304686 4 GCATCGT 3055 0.0 24.921318 8 ACGCAGA 15125 0.0 24.795607 7 TATTCTC 1890 0.0 24.617723 5 CTAGGCA 3865 0.0 24.569332 4 CGCAGAG 15235 0.0 24.524035 8 TAGTACT 3460 0.0 23.912668 4 CGACGAG 1585 0.0 22.97945 24-25 >>END_MODULE