FastQCFastQC Report
Mon 16 Jan 2017
ERR1146484_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146484_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3573946
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA116740.32664175675849605No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC111360.31158836759145214No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN109900.30750324711117627No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC97980.27415075661467747No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT96930.27121282750215026No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA96020.2686666222712934No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC88770.2483809212562249No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC77860.2178544387632046No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT62360.17448500900685127No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC58260.16301309532936425No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG51030.14278335486882007No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT50620.14163616350107136No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT50510.14132838045118756No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC50490.14127241989666325No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA49630.13886611605211718No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC47920.13408148864028724No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC47180.13201094812288713No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA45960.12859735429690322No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG43500.12171420609041099No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC42830.11983952751384602No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG42260.11824465170990273No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA41850.11709746034215401No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT41240.11539066342916204No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT40710.1139077087342674No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC40470.11323618207997546No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC39650.11094179934447805No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT38340.10727638302313466No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG37300.10436643418786964No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA36170.10120466285724519No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT35970.10064505731200192No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG33100.035.4921651
GGGCACC33200.034.4216594
GTATCAA78600.033.134291
GGTATCA51250.032.5889661
CAACGCA73700.032.2524535
TACCCCG2650.031.9084995
ATCAACG77400.030.7765643
GCATCGT36900.030.4088958
TAGGCAT44450.029.80285
TATTCTC23900.027.9106645
TATCAAC93600.027.608712
AACGCAG91300.027.5086546
CTAACGC15200.027.201463
CTAGGCA50750.026.8459244
AGGGCTA47750.026.6609525
ACGCAGA91900.025.4918827
CGCCTAC16500.025.3234547
TAGTACT48250.025.3158474
CGCAGAG93050.025.127088
CTGTGCG48500.024.8796869
Produced by FastQC (version 0.11.5)