##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146484_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3573946 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.486147804135822 31.0 30.0 34.0 27.0 34.0 2 30.57881820262533 31.0 31.0 34.0 26.0 34.0 3 30.762341960398953 31.0 31.0 34.0 27.0 34.0 4 34.234261513744194 37.0 35.0 37.0 30.0 37.0 5 34.45236245875007 37.0 35.0 37.0 30.0 37.0 6 34.65929507608677 37.0 35.0 37.0 31.0 37.0 7 34.55781033065413 37.0 35.0 37.0 30.0 37.0 8 34.49181045264814 37.0 35.0 37.0 30.0 37.0 9 35.87522866881592 39.0 35.0 39.0 30.0 39.0 10-11 35.78360655141404 39.0 35.0 39.0 30.0 39.0 12-13 35.76685014267143 39.0 35.0 39.0 30.0 39.0 14-15 36.79028908103256 40.0 36.0 41.0 29.5 41.0 16-17 36.625859484166796 40.0 36.0 41.0 28.5 41.0 18-19 36.57940424953259 40.0 36.0 41.0 28.0 41.0 20-21 36.91888629542808 40.0 36.0 41.0 30.0 41.0 22-23 36.635955747512696 40.0 36.0 41.0 28.5 41.0 24-25 36.759786381775214 40.0 36.0 41.0 29.5 41.0 26-27 36.835261500873266 40.0 36.0 41.0 30.0 41.0 28-29 36.66823337565817 40.0 35.5 41.0 29.5 41.0 30-31 36.61495920755378 40.0 35.0 41.0 29.5 41.0 32-33 36.7211023893478 40.0 35.5 41.0 30.0 41.0 34-35 36.77629600447237 40.0 35.0 41.0 30.0 41.0 36-37 36.714357323809594 40.0 35.0 41.0 30.0 41.0 38-39 36.542038407967 39.0 35.0 41.0 29.5 41.0 40-41 36.508833373531665 39.0 35.0 41.0 29.0 41.0 42-43 36.35480236690761 39.0 35.0 41.0 29.0 41.0 44-45 36.17086058379169 39.0 35.0 41.0 28.0 41.0 46-47 36.04301477974205 39.0 35.0 41.0 28.0 41.0 48-49 35.91405759907956 38.5 34.5 40.5 28.0 41.0 50-51 35.69428986895717 38.0 34.0 40.0 27.5 41.0 52-53 35.50740246215248 38.0 34.0 40.0 27.0 41.0 54-55 35.32082465711569 38.0 34.0 40.0 27.0 41.0 56-57 35.18099000376615 37.0 33.5 40.0 27.0 41.0 58-59 34.918142159954286 37.0 33.0 40.0 26.5 41.0 60-61 34.660918351872134 36.5 33.0 40.0 26.0 41.0 62-63 34.394042187542844 36.0 33.0 39.0 26.0 41.0 64-65 34.108655670790775 35.0 33.0 39.0 26.0 41.0 66-67 33.81737007218352 35.0 32.0 39.0 26.0 41.0 68-69 33.533841026137495 35.0 32.0 38.0 26.0 40.0 70-71 33.467478943442345 35.0 32.0 37.5 26.0 40.0 72-73 33.26661049159668 35.0 32.0 37.0 26.0 40.0 74-75 32.99141215340131 35.0 32.0 36.5 25.0 39.5 76-77 32.764273019234196 35.0 32.0 36.0 25.0 39.0 78-79 32.55249967402977 35.0 32.0 36.0 25.0 39.0 80-81 32.334114309505516 35.0 31.5 35.5 24.5 38.0 82-83 32.10396072016757 35.0 31.0 35.0 24.0 37.0 84-85 31.92942338804224 35.0 31.0 35.0 24.0 37.0 86-87 31.763862408665382 35.0 31.0 35.0 24.0 36.0 88-89 31.76416949220833 35.0 32.0 35.0 24.0 36.0 90-91 31.699031406742016 35.0 32.0 35.0 24.0 36.0 92-93 31.5628224377201 35.0 31.0 35.0 24.0 36.0 94-95 31.55460798792147 35.0 31.5 35.0 24.0 35.0 96-97 31.481355062443583 35.0 31.0 35.0 24.0 35.0 98-99 31.41674552441475 35.0 31.0 35.0 24.0 35.0 100 30.94166447954166 34.0 30.0 35.0 22.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7834842155333668 1101 2 0.9402486965364716 1101 3 0.914653346953969 1101 4 1.0354771364683728 1101 5 0.8799522620308551 1101 6 0.5864499390721463 1101 7 0.690383068764092 1101 8 0.7287710280252213 1101 9 0.8929535599985456 1101 10-11 0.9117825117538345 1101 12-13 0.8425060917584162 1101 14-15 1.0964927049901974 1101 16-17 1.186557614499712 1101 18-19 1.2841876490469062 1101 20-21 0.9453698246940192 1101 22-23 1.248384233945636 1101 24-25 1.1455472363667525 1101 26-27 1.0191731186575055 1101 28-29 1.2417355371620502 1101 30-31 1.244567387368761 1101 32-33 1.1488848117181476 1101 34-35 1.0314988043147295 1101 36-37 0.9585875380354523 1101 38-39 1.0840152742920282 1101 40-41 0.9884656631240176 1101 42-43 0.96333722585873 1101 44-45 0.9428034727087322 1101 46-47 1.0391773162484483 1101 48-49 0.9709849326311115 1101 50-51 1.0170802477980772 1101 52-53 0.9626184874894292 1101 54-55 1.0060768532562534 1101 56-57 0.8496014949019681 1101 58-59 0.7774899232571073 1101 60-61 0.8662717424954209 1101 62-63 0.7562435622983799 1101 64-65 0.7530283835912641 1101 66-67 0.8110029951316804 1101 68-69 0.7728199213958931 1101 70-71 0.7123717739457831 1101 72-73 0.7100454704922541 1101 74-75 0.7281868601786599 1101 76-77 0.6936523608149088 1101 78-79 0.715073979618893 1101 80-81 0.7324688215952904 1101 82-83 0.6982234222563832 1101 84-85 0.7619490884758164 1101 86-87 0.752140098463201 1101 88-89 0.695939349686121 1101 90-91 0.7803484020524287 1101 92-93 0.8780957999357 1101 94-95 0.7978782883510114 1101 96-97 0.7436530284500904 1101 98-99 0.7976808717934887 1101 100 0.8861605479402499 1102 1 0.8262573339486501 1102 2 0.9634460593796064 1102 3 0.9413878044216197 1102 4 1.0298361089158234 1102 5 0.9067456972027443 1102 6 0.6047305540134715 1102 7 0.7288944297057967 1102 8 0.7049067956061066 1102 9 0.8473188210786944 1102 10-11 0.8592553763490045 1102 12-13 0.8683017307734815 1102 14-15 1.0274245008111222 1102 16-17 1.1862952107447526 1102 18-19 1.2039905123256105 1102 20-21 1.0109657295999526 1102 22-23 1.2594679839229386 1102 24-25 1.1125650613240623 1102 26-27 1.0581638955269455 1102 28-29 1.2066407375568247 1102 30-31 1.1845193579887408 1102 32-33 1.065612769794022 1102 34-35 0.9500737782668196 1102 36-37 0.961729502943335 1102 38-39 1.036549805424329 1102 40-41 0.9262907437532988 1102 42-43 0.9973292852232589 1102 44-45 1.0155402956247457 1102 46-47 0.976048860126248 1102 48-49 0.9707952494653966 1102 50-51 1.013567137904431 1102 52-53 0.9717482744245487 1102 54-55 0.9716311567596989 1102 56-57 0.8899911372187361 1102 58-59 0.7657896420278689 1102 60-61 0.8732295265473553 1102 62-63 0.8996777047735307 1102 64-65 0.8056562130622993 1102 66-67 0.8590942791013774 1102 68-69 0.9364117583381883 1102 70-71 0.8517673197112785 1102 72-73 0.7453034777432705 1102 74-75 0.766762197820789 1102 76-77 0.7688044827076439 1102 78-79 0.8304029294189803 1102 80-81 0.8465493461227211 1102 82-83 0.7347895814628131 1102 84-85 0.7521440538561137 1102 86-87 0.7583984759186251 1102 88-89 0.7152222894916278 1102 90-91 0.71494799795936 1102 92-93 0.8262079960825162 1102 94-95 0.7944234942735982 1102 96-97 0.8201919798794606 1102 98-99 0.8039812268824633 1102 100 0.7721632297014018 1103 1 0.7040745097415453 1103 2 0.808907861943581 1103 3 0.7777639859861694 1103 4 0.7585602997231362 1103 5 0.7276781238899162 1103 6 0.5697352326256606 1103 7 0.5850172021845594 1103 8 0.6714082001765149 1103 9 0.813878866886526 1103 10-11 0.8521200698696063 1103 12-13 0.7643060423140753 1103 14-15 0.8573521550037455 1103 16-17 1.1130272663851386 1103 18-19 1.2285555346036432 1103 20-21 0.8842118315082743 1103 22-23 1.1563254126963969 1103 24-25 1.0804104920439528 1103 26-27 0.9398363626483786 1103 28-29 1.0615215245729743 1103 30-31 1.0727661985511077 1103 32-33 0.9177002745022378 1103 34-35 0.9001906131793262 1103 36-37 0.811320931398761 1103 38-39 0.9268939864938162 1103 40-41 0.8528988915867259 1103 42-43 0.8957478254962581 1103 44-45 0.924731343983467 1103 46-47 0.9626233118024814 1103 48-49 0.9629888109394642 1103 50-51 1.0262736791089253 1103 52-53 1.0561220374609448 1103 54-55 1.0318847815211853 1103 56-57 0.8802193890726997 1103 58-59 0.8846824954653698 1103 60-61 0.8979332402856457 1103 62-63 0.825160092385353 1103 64-65 0.8291388569476013 1103 66-67 0.8506977983650259 1103 68-69 0.9075408164245857 1103 70-71 0.8461974593901971 1103 72-73 0.8263229197098738 1103 74-75 0.8119122679365418 1103 76-77 0.7392242426056086 1103 78-79 0.7770021358733459 1103 80-81 0.8038092405628561 1103 82-83 0.7145697726783524 1103 84-85 0.7503411001139 1103 86-87 0.7720336208526959 1103 88-89 0.7728072445746861 1103 90-91 0.8279998282144057 1103 92-93 0.7729809236786309 1103 94-95 0.7733013540206777 1103 96-97 0.7627144926170146 1103 98-99 0.8163711265495621 1103 100 0.9138433843225542 1104 1 0.4034545984212663 1104 2 0.5853223018401579 1104 3 0.539324371928906 1104 4 0.7023558114467434 1104 5 0.6258790030499597 1104 6 0.3703706050649984 1104 7 0.368802030171139 1104 8 0.40656213619695336 1104 9 0.43648083694439066 1104 10-11 0.4640775609927772 1104 12-13 0.3820636321670463 1104 14-15 0.6226181249907725 1104 16-17 0.7008574736900428 1104 18-19 0.7184173813765469 1104 20-21 0.6093397506505553 1104 22-23 0.831459734194695 1104 24-25 0.7014707083640275 1104 26-27 0.61032012134811 1104 28-29 0.8138355214306685 1104 30-31 0.8145513116863725 1104 32-33 0.6172771897993741 1104 34-35 0.5746341132003607 1104 36-37 0.523786453449155 1104 38-39 0.6324070376152591 1104 40-41 0.5834142670643274 1104 42-43 0.469907204107308 1104 44-45 0.5350117706356414 1104 46-47 0.554041467210034 1104 48-49 0.5187747767537161 1104 50-51 0.6163740248845642 1104 52-53 0.6333723773980324 1104 54-55 0.5312294306872971 1104 56-57 0.5128915456933569 1104 58-59 0.4913372378160261 1104 60-61 0.4895248749831893 1104 62-63 0.44345795668943566 1104 64-65 0.39181007068211215 1104 66-67 0.5085793441946933 1104 68-69 0.41935048264704733 1104 70-71 0.3584265473792527 1104 72-73 0.4428037566385754 1104 74-75 0.3684441203321782 1104 76-77 0.3621493742637796 1104 78-79 0.3879705131462359 1104 80-81 0.4014310748727894 1104 82-83 0.3147834197481103 1104 84-85 0.29582285267289876 1104 86-87 0.32193277423364464 1104 88-89 0.30868171024948055 1104 90-91 0.32659184601564917 1104 92-93 0.384907527152329 1104 94-95 0.3820429496745703 1104 96-97 0.35695968078471907 1104 98-99 0.30669803479062097 1104 100 0.4753546682415539 1105 1 0.4399848775215851 1105 2 0.6113833884120474 1105 3 0.6072221192214435 1105 4 0.6677128665118843 1105 5 0.57576934990648 1105 6 0.3515090027450043 1105 7 0.4110795231444868 1105 8 0.44264250867389165 1105 9 0.41853338470214396 1105 10-11 0.5759143893700127 1105 12-13 0.5275891797420655 1105 14-15 0.6676528941672899 1105 16-17 0.8001201591457985 1105 18-19 0.8034446225361123 1105 20-21 0.5702930203740166 1105 22-23 0.8616454595754348 1105 24-25 0.7849320471089669 1105 26-27 0.4923540109853448 1105 28-29 0.7523936997342275 1105 30-31 0.7447931666037846 1105 32-33 0.6638995859061865 1105 34-35 0.4889282170622309 1105 36-37 0.4436581749068651 1105 38-39 0.60517722944779 1105 40-41 0.4520906156009872 1105 42-43 0.5661749593026499 1105 44-45 0.6441027079402062 1105 46-47 0.5464323206626958 1105 48-49 0.5687100265649008 1105 50-51 0.6100004095652167 1105 52-53 0.5781483043677653 1105 54-55 0.5590602388099342 1105 56-57 0.5849189749858752 1105 58-59 0.5609893746835795 1105 60-61 0.5645955749568685 1105 62-63 0.5075557859616637 1105 64-65 0.4227472821428435 1105 66-67 0.47416993780325356 1105 68-69 0.46354381477922857 1105 70-71 0.4219911140060333 1105 72-73 0.4194369485477907 1105 74-75 0.44021507215168043 1105 76-77 0.44611287316435266 1105 78-79 0.4823580013904518 1105 80-81 0.598875195724645 1105 82-83 0.5894418666766583 1105 84-85 0.5035509847973572 1105 86-87 0.45323201935057966 1105 88-89 0.49788976023503295 1105 90-91 0.5442234899181848 1105 92-93 0.5847542816092002 1105 94-95 0.5201267036870725 1105 96-97 0.4991360771726576 1105 98-99 0.5256651123328737 1105 100 0.6312773193854007 1106 1 0.5317267699518311 1106 2 0.5484125915766533 1106 3 0.5353149703578168 1106 4 0.6018716463721603 1106 5 0.5570615281528504 1106 6 0.313679581205875 1106 7 0.3247940645334637 1106 8 0.34349777710234264 1106 9 0.566135277372183 1106 10-11 0.5872276489054329 1106 12-13 0.4513707417275654 1106 14-15 0.576436313862196 1106 16-17 0.7444978224076024 1106 18-19 0.7429598489409628 1106 20-21 0.565469822263573 1106 22-23 0.8019298267225707 1106 24-25 0.640002527663718 1106 26-27 0.5095020585034717 1106 28-29 0.7987120085333501 1106 30-31 0.7769593334305895 1106 32-33 0.6605915756430747 1106 34-35 0.5504416793584355 1106 36-37 0.4375525135459597 1106 38-39 0.61659982611544 1106 40-41 0.5901849293343844 1106 42-43 0.5967119687978837 1106 44-45 0.5983078719499986 1106 46-47 0.5832107943681066 1106 48-49 0.5738004705131701 1106 50-51 0.5156610619856821 1106 52-53 0.4722953820893636 1106 54-55 0.5711663287996345 1106 56-57 0.47466201433197597 1106 58-59 0.4509420620199833 1106 60-61 0.45318176953963274 1106 62-63 0.41617256019880955 1106 64-65 0.5105133562681061 1106 66-67 0.5223381048524018 1106 68-69 0.4189089231408474 1106 70-71 0.3848090125695123 1106 72-73 0.36198096679162717 1106 74-75 0.4139667861219323 1106 76-77 0.42972664318496356 1106 78-79 0.4399870014945435 1106 80-81 0.502080275807117 1106 82-83 0.4646860018465091 1106 84-85 0.425801043519229 1106 86-87 0.469058824700781 1106 88-89 0.512772979321845 1106 90-91 0.4761548578081367 1106 92-93 0.4779242396073897 1106 94-95 0.5009079607548195 1106 96-97 0.520278608649491 1106 98-99 0.46453369986334536 1106 100 0.5103219758038513 1107 1 0.3420933607883079 1107 2 0.5284507528324482 1107 3 0.45448994984749547 1107 4 0.56786085358587 1107 5 0.450302698849697 1107 6 0.14780108451794405 1107 7 0.16542625924478926 1107 8 0.2286214774987414 1107 9 0.2529332406985745 1107 10-11 0.28185906184055654 1107 12-13 0.18812723227856765 1107 14-15 0.36942285583012335 1107 16-17 0.47873914395190553 1107 18-19 0.718675788653492 1107 20-21 0.4714939449780573 1107 22-23 0.5991007758038123 1107 24-25 0.3599262880428711 1107 26-27 0.3380980029674916 1107 28-29 0.5211686806493461 1107 30-31 0.4990448125610243 1107 32-33 0.447046549389313 1107 34-35 0.29901100904219646 1107 36-37 0.3199476526407494 1107 38-39 0.39582782280090356 1107 40-41 0.30322553799086194 1107 42-43 0.45657974915082633 1107 44-45 0.3205263016731621 1107 46-47 0.35712838849774187 1107 48-49 0.379771625355275 1107 50-51 0.39878497173259575 1107 52-53 0.4030994195948949 1107 54-55 0.40196430706006936 1107 56-57 0.34564123081302256 1107 58-59 0.3611514481571447 1107 60-61 0.37794812381550713 1107 62-63 0.34843314568971806 1107 64-65 0.4001083959214 1107 66-67 0.3342853914066737 1107 68-69 0.3788514558765286 1107 70-71 0.2451729899497863 1107 72-73 0.24253392753950465 1107 74-75 0.29083818174453313 1107 76-77 0.23291184687150945 1107 78-79 0.21957916942873368 1107 80-81 0.2533594478696699 1107 82-83 0.30976838497328885 1107 84-85 0.3678158832921028 1107 86-87 0.31325062895695055 1107 88-89 0.3107320070421551 1107 90-91 0.3028579475367543 1107 92-93 0.4104216117651944 1107 94-95 0.41869575331804754 1107 96-97 0.41056544351632596 1107 98-99 0.41176363495920754 1107 100 0.4964563404716209 1108 1 0.32409782394527653 1108 2 0.40903392258031346 1108 3 0.3973787652549028 1108 4 0.492033854770618 1108 5 0.35351444805747434 1108 6 0.14000661094730305 1108 7 0.17084729071032 1108 8 0.19897279009298785 1108 9 0.22835141465918696 1108 10-11 0.31467836913199676 1108 12-13 0.1657715556666517 1108 14-15 0.33551021738327336 1108 16-17 0.5672606606537371 1108 18-19 0.5111210501016998 1108 20-21 0.2708974366103263 1108 22-23 0.5018423383241952 1108 24-25 0.3854711815898568 1108 26-27 0.30095047269274033 1108 28-29 0.3958250567463608 1108 30-31 0.5488525328959284 1108 32-33 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-3.1904443829207274 2216 1 -0.5718368084533658 2216 2 -0.4659937490403685 2216 3 -0.7511543080231391 2216 4 -0.6041689035768982 2216 5 -0.7295832886674916 2216 6 -0.6312011210025616 2216 7 -0.7552888269730715 2216 8 -0.5871689819090093 2216 9 -0.826063047118538 2216 10-11 -0.870289200962155 2216 12-13 -1.012103023876108 2216 14-15 -1.3575075234058147 2216 16-17 -1.024608699466249 2216 18-19 -2.9732234851821673 2216 20-21 -1.2691211378599192 2216 22-23 -0.8002572956590441 2216 24-25 -1.0396762153489192 2216 26-27 -0.9797445318816287 2216 28-29 -0.8850065676938428 2216 30-31 -1.0313130493721658 2216 32-33 -1.0029404217907185 2216 34-35 -1.1509590627101076 2216 36-37 -1.2060925506913023 2216 38-39 -1.240825711868304 2216 40-41 -1.3398931892580563 2216 42-43 -1.3730295784737834 2216 44-45 -1.1787297750648804 2216 46-47 -1.2534903291690327 2216 48-49 -1.3198470428327624 2216 50-51 -1.206614116673947 2216 52-53 -1.277679101615739 2216 54-55 -1.05970167859644 2216 56-57 -1.1558835650610604 2216 58-59 -1.1401265057321623 2216 60-61 -1.1464864161060007 2216 62-63 -1.1222435886997104 2216 64-65 -1.1060572673683637 2216 66-67 -1.1985774767641928 2216 68-69 -1.2792353587486005 2216 70-71 -1.3093133202942013 2216 72-73 -1.2416542622809104 2216 74-75 -1.3018187671898076 2216 76-77 -1.2264072234996846 2216 78-79 -1.2533129434631576 2216 80-81 -1.1809348366741794 2216 82-83 -1.3450562448450327 2216 84-85 -1.2662799287974096 2216 86-87 -1.2961198394847315 2216 88-89 -1.1640244727849804 2216 90-91 -1.112967295074565 2216 92-93 -1.0978845585933428 2216 94-95 -1.049708850109294 2216 96-97 -1.0712583653730974 2216 98-99 -1.0010280732639885 2216 100 -1.0242512368252576 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 14846.0 1 2392.0 2 372.0 3 365.0 4 332.0 5 205.0 6 166.0 7 243.0 8 431.0 9 834.0 10 1270.0 11 2266.0 12 3503.0 13 5191.0 14 7025.0 15 9124.0 16 11123.0 17 13323.0 18 15509.0 19 17620.0 20 20369.0 21 23732.0 22 27642.0 23 32014.0 24 36295.0 25 40969.0 26 46833.0 27 52636.0 28 63130.0 29 75045.0 30 89452.0 31 107316.0 32 132696.0 33 171228.0 34 236672.0 35 320233.0 36 469121.0 37 716510.0 38 596356.0 39 209383.0 40 174.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.19364729078296 21.31640204581837 12.87912532672497 23.61082533667369 2 15.337388482006192 19.774639637588653 41.058520843205606 23.829451037199554 3 18.522060065998993 26.84779042269551 28.950120068456307 25.680029442849182 4 12.184933834734846 16.97725954668648 40.13385137701284 30.70395524156584 5 13.228078842790753 38.45647355584202 34.95953123054791 13.35591637081932 6 29.687710002693656 38.42410323402039 18.32674352495795 13.561443238327996 7 26.893744134713245 31.85634269332562 23.75241114784839 17.497502024112745 8 26.12516283475338 34.98179850563257 20.10342678567009 18.789611873943965 9 26.072116477240765 15.269889719697668 21.75549545130318 36.90249835175839 10-11 25.58172245037809 26.044473000263586 27.506680345704197 20.867124203654125 12-13 25.869624565794275 23.855410705839148 27.499932839378094 22.775031888988483 14-15 23.31414054979744 24.964781818867156 24.992348765760276 26.72872886557513 16-17 22.161176901154402 27.551973696610272 26.436068478156606 23.850780924078723 18-19 21.69488488329113 28.156799292472336 27.964234859127394 22.184080965109143 20-21 23.425707149932848 27.201219492469807 26.777961201112316 22.59511215648503 22-23 23.85153220194952 26.89495868559902 26.52866939213721 22.72483972031425 24-25 23.20957225797096 27.062109887210383 27.272968931061946 22.455348923756706 26-27 23.042190191095557 27.26856367283253 26.374452007870165 23.314794128201754 28-29 22.23630367046261 27.805455511178366 26.84204819820979 23.11619262014923 30-31 23.106498906732806 27.259423558206425 26.752363589111543 22.88171394594923 32-33 23.55906004807739 27.64991346041744 25.733793358918543 23.05723313258663 34-35 23.551283827452128 27.359537089325357 26.77680580101114 22.31237328221137 36-37 23.226521637598015 27.072755561682836 26.562940496253763 23.137782304465386 38-39 23.379260162209373 26.942836240385184 27.12325017403488 22.554653423370567 40-41 22.94676496934559 26.241270132430657 27.704331792360254 23.1076331058635 42-43 22.900921311213914 26.49263824556305 27.021312240763855 23.58512820245918 44-45 22.983025248646634 27.501564702285936 26.80883312247894 22.706576926588486 46-47 23.000944505451375 27.107841027099738 26.393877061234733 23.497337406214154 48-49 23.705191973972834 26.56419447692027 26.653106280545853 23.077507268561043 50-51 23.3477981449164 26.583356741187803 26.48679494592891 23.582050167966887 52-53 22.728302646285805 27.25844138181095 26.398371421221622 23.614884550681627 54-55 22.752637667329832 27.175991150761142 26.335009632437007 23.736361549472015 56-57 23.753282113446886 26.61455228168153 26.172239631657877 23.459925973213707 58-59 22.818248308648748 26.87103534607649 27.074869544425223 23.235846800849544 60-61 22.785065777648512 27.556009240853168 26.331677769548563 23.327247211949764 62-63 23.274586279721895 26.791407292356517 25.911600819121922 24.02240560879967 64-65 22.3893530604733 27.00633309042914 26.370647046421425 24.233666802676137 66-67 23.200301549025276 26.514893554178613 26.35278282351998 23.932022073276134 68-69 22.595291927716673 26.692431228539693 26.542722542903736 24.1695543008399 70-71 22.625745667564377 26.32848770438827 26.799135081184243 24.24663154686311 72-73 23.243820179992174 26.535912280644997 27.138235234957435 23.08203230440539 74-75 23.35452900912853 26.894132318461477 26.67955933136497 23.07177934104502 76-77 23.048085488563334 26.843340818167473 26.339738899957215 23.768834793311974 78-79 22.80234907552702 26.718300296645065 26.573086867230582 23.90626376059733 80-81 22.482822989057993 27.010470835263146 27.152834925869268 23.353871249809597 82-83 23.348781081267344 25.985971850874613 28.085679445400064 22.579567622457976 84-85 23.64046272028564 26.632958938997085 26.612788315779685 23.113790024937586 86-87 22.396331804259987 27.246867953503866 27.142441711574467 23.21435853066168 88-89 23.359041867233838 25.987718590095387 26.892617670906716 23.760621871764062 90-91 23.087879110679545 25.971305224795167 27.242834122221087 23.697981542304202 92-93 23.30754973168135 26.295369222137627 27.066953333463484 23.330127712717545 94-95 22.078801051261667 26.234530043960074 27.77575706388054 23.91091184089771 96-97 22.489819568779197 26.256529608199457 27.25099074063857 24.002660082382768 98-99 21.928433194710127 27.016969047638916 27.084458211674367 23.970139545976586 100 22.74554169435559 26.276370958867062 26.333488645914755 24.644598700862595 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 12427.0 1 7362.5 2 2102.0 3 1538.0 4 879.0 5 495.5 6 374.0 7 344.0 8 365.5 9 380.5 10 344.0 11 306.0 12 304.0 13 320.5 14 333.5 15 450.5 16 526.0 17 562.5 18 703.5 19 869.0 20 1088.5 21 1395.0 22 1921.5 23 2677.0 24 3545.0 25 4764.5 26 6684.5 27 8932.5 28 12764.5 29 20738.5 30 31192.0 31 41086.0 32 54675.0 33 66545.5 34 73981.0 35 83391.5 36 92179.5 37 109596.5 38 117341.5 39 106529.0 40 97000.5 41 88627.0 42 86681.0 43 93322.5 44 103492.5 45 123535.0 46 141903.5 47 154442.0 48 163752.5 49 161319.5 50 153836.5 51 156746.0 52 159297.5 53 154016.0 54 146472.0 55 128498.0 56 114368.5 57 97113.5 58 78878.0 59 73962.0 60 62324.0 61 45307.0 62 34627.0 63 28476.0 64 20828.0 65 11459.0 66 7067.0 67 5120.5 68 3784.5 69 2726.0 70 2048.5 71 1629.5 72 1280.5 73 1049.5 74 840.5 75 670.5 76 500.0 77 331.5 78 228.0 79 150.5 80 98.5 81 72.5 82 53.0 83 37.5 84 29.0 85 22.0 86 18.0 87 14.5 88 11.0 89 8.5 90 9.0 91 9.0 92 5.5 93 4.0 94 5.0 95 5.5 96 5.0 97 4.5 98 3.0 99 2.5 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 1.0017498865399757 2 0.4476844361946152 3 0.48196027584076534 4 0.4677462949915863 5 0.4343378439405632 6 0.4880879565611791 7 0.505519669295507 8 0.5090731645078017 9 0.521608328721251 10-11 0.5348429998662543 12-13 0.5326185678239123 14-15 0.5306179779996676 16-17 0.534954920975303 18-19 0.5325206368534947 20-21 0.5323107847740285 22-23 0.49638410876941064 24-25 0.5306319681382987 26-27 0.510304296707337 28-29 0.515970302852925 30-31 0.5189781826586076 32-33 0.521454437196309 34-35 0.5329823114283204 36-37 0.5516591465008145 38-39 0.5412085129433964 40-41 0.5451117616214682 42-43 0.5426075268065047 44-45 0.55276436745267 46-47 0.5513513634509307 48-49 0.5242804451997876 50-51 0.5379907810582477 52-53 0.5300723625930555 54-55 0.5397395483871329 56-57 0.528309605125539 58-59 0.5343113745982732 60-61 0.5277919699961892 62-63 0.5225456680095334 64-65 0.532926350873796 66-67 0.5307438892473473 68-69 0.5514912648372415 70-71 0.5361440827589449 72-73 0.539781518803026 74-75 0.537543096622053 76-77 0.5248260606063998 78-79 0.5558841683674012 80-81 0.5309677314654446 82-83 0.5302542343952595 84-85 0.5392219132577828 86-87 0.8477044700731349 88-89 1.1165669542852634 90-91 1.1063261728073115 92-93 1.106032379896059 94-95 1.1112786818827145 96-97 1.1043535632603292 98-99 1.0997508076507032 100 1.0951200717638152 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3573946.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.94408652393019 #Duplication Level Percentage of deduplicated Percentage of total 1 75.36786060741272 24.075574603667015 2 12.540322386707336 8.011782867179159 3 4.605444787587856 4.413501803276692 4 2.33828756414752 2.9877784106783336 5 1.271795010618146 2.0313164929944385 6 0.7666448435070162 1.4693861528467826 7 0.5314147535663688 1.1882891207611985 8 0.3630690163121472 0.9278326456986763 9 0.2732080459202461 0.7854643312119212 >10 1.4728137680655407 8.861995935688913 >50 0.18035395432958085 4.066841105095849 >100 0.22290412345967597 15.625182568522428 >500 0.04135092727227107 9.252020298879804 >1k 0.023303700539032475 13.171927229441108 >5k 9.636868643960797E-4 2.1851951028354324 >10k+ 2.6282369028983996E-4 0.9459113312223867 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 11674 0.32664175675849605 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 11136 0.31158836759145214 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 10990 0.30750324711117627 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 9798 0.27415075661467747 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 9693 0.27121282750215026 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 9602 0.2686666222712934 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 8877 0.2483809212562249 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 7786 0.2178544387632046 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 6236 0.17448500900685127 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 5826 0.16301309532936425 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 5103 0.14278335486882007 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 5062 0.14163616350107136 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 5051 0.14132838045118756 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 5049 0.14127241989666325 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 4963 0.13886611605211718 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 4792 0.13408148864028724 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 4718 0.13201094812288713 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 4596 0.12859735429690322 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 4350 0.12171420609041099 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 4283 0.11983952751384602 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 4226 0.11824465170990273 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 4185 0.11709746034215401 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 4124 0.11539066342916204 No Hit GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT 4071 0.1139077087342674 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 4047 0.11323618207997546 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 3965 0.11094179934447805 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 3834 0.10727638302313466 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 3730 0.10436643418786964 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 3617 0.10120466285724519 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 3597 0.10064505731200192 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 8.394083178649034E-5 2 0.0 0.0 0.0 0.0 8.394083178649034E-5 3 0.0 0.0 0.0 0.0 8.394083178649034E-5 4 0.0 0.0 0.0 0.0 8.394083178649034E-5 5 0.0 0.0 0.0 0.0 8.394083178649034E-5 6 2.7980277262163447E-5 0.0 0.0 0.0 1.3990138631081724E-4 7 2.7980277262163447E-5 0.0 0.0 0.0 1.3990138631081724E-4 8 2.7980277262163447E-5 0.0 0.0 0.0 1.3990138631081724E-4 9 2.7980277262163447E-5 0.0 0.0 2.7980277262163447E-5 1.3990138631081724E-4 10-11 2.7980277262163447E-5 0.0 0.0 2.7980277262163447E-5 1.6788166357298068E-4 12-13 2.7980277262163447E-5 0.0 0.0 2.7980277262163447E-5 2.6581263399055275E-4 14-15 2.7980277262163447E-5 0.0 0.0 2.7980277262163447E-5 3.637436044081248E-4 16-17 2.7980277262163447E-5 0.0 0.0 4.197041589324517E-5 5.03644990718942E-4 18-19 2.7980277262163447E-5 0.0 0.0 5.5960554524326894E-5 5.316252679811055E-4 20-21 2.7980277262163447E-5 0.0 0.0 1.1192110904865379E-4 6.155660997675958E-4 22-23 2.7980277262163447E-5 0.0 0.0 1.958619408351441E-4 7.274872088162496E-4 24-25 2.7980277262163447E-5 0.0 0.0 3.7773374303920655E-4 7.974379019716582E-4 26-27 2.7980277262163447E-5 0.0 0.0 5.176351293500238E-4 8.254181792338216E-4 28-29 2.7980277262163447E-5 0.0 0.0 0.0013010828926906 8.394083178649034E-4 30-31 2.7980277262163447E-5 0.0 0.0 0.005106400600344829 8.394083178649034E-4 32-33 2.7980277262163447E-5 0.0 0.0 0.015445113048714224 8.953688723892303E-4 34-35 2.7980277262163447E-5 0.0 0.0 0.030988157067846016 8.953688723892303E-4 36-37 2.7980277262163447E-5 0.0 0.0 0.058940454052747296 8.953688723892303E-4 38-39 2.7980277262163447E-5 0.0 0.0 0.10268761755213984 9.233491496513938E-4 40-41 2.7980277262163447E-5 0.0 0.0 0.1517930041472367 9.233491496513938E-4 42-43 2.7980277262163447E-5 0.0 0.0 0.20474567886588102 9.233491496513938E-4 44-45 2.7980277262163447E-5 0.0 0.0 0.26943607989600293 9.513294269135571E-4 46-47 2.7980277262163447E-5 0.0 0.0 0.3533909018211243 0.001007289981437884 48-49 2.7980277262163447E-5 0.0 0.0 0.45382610705366 0.001063250535962211 50-51 2.7980277262163447E-5 0.0 0.0 0.5656352949932651 0.0011471913677487014 52-53 2.7980277262163447E-5 0.0 0.0 0.6744645834044498 0.0011751716450108646 54-55 2.7980277262163447E-5 0.0 0.0 0.7969762273968326 0.0012870927540595185 56-57 2.7980277262163447E-5 0.0 0.0 0.9511755353886153 0.001315073031321682 58-59 2.7980277262163447E-5 0.0 0.0 1.1358733455961563 0.0013710335858460089 60-61 2.7980277262163447E-5 0.0 0.0 1.3315254343518341 0.0013710335858460089 62-63 2.7980277262163447E-5 0.0 0.0 1.5370125905651624 0.0014549744176324993 64-65 2.7980277262163447E-5 0.0 0.0 1.754685157526163 0.0014549744176324993 66-67 2.7980277262163447E-5 0.0 0.0 2.0031779998914367 0.0014549744176324993 68-69 2.7980277262163447E-5 0.0 0.0 2.2919484513755943 0.0014549744176324993 70-71 2.7980277262163447E-5 0.0 0.0 2.60979041093514 0.0014969448335257446 72-73 2.7980277262163447E-5 0.0 0.0 2.943217384929711 0.001664826497098725 74-75 2.7980277262163447E-5 0.0 0.0 3.274825640902241 0.0016788166357298068 76-77 2.7980277262163447E-5 0.0 0.0 3.657330021214646 0.0017067969129919702 78-79 2.7980277262163447E-5 0.0 0.0 4.0720956612103265 0.0017907377447784606 80-81 2.7980277262163447E-5 0.0 0.0 4.527390732820249 0.0018886687151960326 82-83 2.7980277262163447E-5 0.0 0.0 5.003111406831552 0.0020565503787690134 84-85 2.7980277262163447E-5 0.0 0.0 5.4656673603910075 0.0020985207946622583 86-87 2.7980277262163447E-5 0.0 0.0 5.962499153596613 0.002210441903710912 88 2.7980277262163447E-5 0.0 0.0 6.359525297808081 0.002322363012759566 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 3310 0.0 35.492165 1 GGGCACC 3320 0.0 34.42165 94 GTATCAA 7860 0.0 33.13429 1 GGTATCA 5125 0.0 32.588966 1 CAACGCA 7370 0.0 32.252453 5 TACCCCG 265 0.0 31.908499 5 ATCAACG 7740 0.0 30.776564 3 GCATCGT 3690 0.0 30.408895 8 TAGGCAT 4445 0.0 29.8028 5 TATTCTC 2390 0.0 27.910664 5 TATCAAC 9360 0.0 27.60871 2 AACGCAG 9130 0.0 27.508654 6 CTAACGC 1520 0.0 27.20146 3 CTAGGCA 5075 0.0 26.845924 4 AGGGCTA 4775 0.0 26.660952 5 ACGCAGA 9190 0.0 25.491882 7 CGCCTAC 1650 0.0 25.323454 7 TAGTACT 4825 0.0 25.315847 4 CGCAGAG 9305 0.0 25.12708 8 CTGTGCG 4850 0.0 24.879686 9 >>END_MODULE