FastQCFastQC Report
Mon 16 Jan 2017
ERR1146484_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146484_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3573946
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC128060.3583154306192651No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA126440.3537826257027946No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC106200.29715054452417583No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA103570.28979173160422683No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC102390.2864900588872915No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT95080.26603647620865006No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC87810.2456948146390572No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84310.23590171759730003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82780.23162073517618903No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC64880.18153603887691644No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT62890.17596796370174592No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG53400.1494146805799528No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC51680.1446020728908607No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT50380.14096463684677943No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC49820.1393977413200983No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG49800.13934178076557396No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG48670.13618000943494948No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA48650.13612404888042517No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT48600.13598414749411436No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC47020.13156326368669252No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA46540.13022021037810866No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG45130.12627499128414363No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC44990.12588326740247335No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44930.12571538573890034No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA44900.12563144490711387No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT42250.11821667143264057No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA41900.11723736172846483No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT41520.11617411119250264No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA40980.1146631762203458No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC39670.11099775989900239No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT39400.11024229241292398No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT39340.11007441074935101No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA38680.10822771245004821No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC38660.10817175189552389No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT38640.10811579134099955No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC37880.10598929026907514No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG37280.10431047363334532No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT36510.10215599228415874No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA79800.047.4333231
GTATCAA135600.038.7679331
GTGGTAT21200.037.261811
GGGCACC35400.034.5457394
CAACGCA161550.031.1855555
ATCAACG162800.031.0146923
TATTCTC23750.030.4735325
GTACTAG36000.030.4329431
TATCAAC175300.029.902712
TAGGCAT43400.029.5623255
AACGCAG184400.028.2386886
GGACCGT1704.08545E-927.6450086
TAGTACT48950.027.0836944
TCAACGC191550.026.6538054
CTGTGCG49050.026.6406239
CTAGGCA51000.026.5481224
ACGCAGA190900.026.3170667
CGCAGAG193450.025.9697978
CTAACGC17600.025.9105533
TCTAACG17850.025.5476592
Produced by FastQC (version 0.11.5)