##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146484_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3573946
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.835722196138384	31.0	31.0	34.0	30.0	34.0
2	31.83996512538242	31.0	31.0	34.0	30.0	34.0
3	32.009126047231824	33.0	31.0	34.0	30.0	34.0
4	35.73967205995837	37.0	35.0	37.0	35.0	37.0
5	35.64785981657249	37.0	35.0	37.0	33.0	37.0
6	35.67629169550967	37.0	35.0	37.0	33.0	37.0
7	35.59401317199532	37.0	35.0	37.0	33.0	37.0
8	35.57796256574666	37.0	35.0	37.0	33.0	37.0
9	37.1762085940862	39.0	37.0	39.0	33.0	39.0
10-11	37.11623692691496	39.0	37.0	39.0	33.0	39.0
12-13	37.05386790958789	39.0	37.0	39.0	33.0	39.0
14-15	38.23219824809888	40.0	37.0	41.0	33.0	41.0
16-17	38.23987141943387	40.0	37.5	41.0	33.0	41.0
18-19	38.3012555310013	40.0	38.0	41.0	33.0	41.0
20-21	38.32003827142324	40.0	38.0	41.0	33.0	41.0
22-23	38.2909467574496	40.0	38.0	41.0	33.0	41.0
24-25	38.22123375674954	40.0	37.0	41.0	33.0	41.0
26-27	38.068972782465096	40.0	37.0	41.0	33.0	41.0
28-29	38.00681473642859	40.0	37.0	41.0	33.0	41.0
30-31	37.8742580329977	40.0	37.0	41.0	33.0	41.0
32-33	37.743021299146655	40.0	36.5	41.0	33.0	41.0
34-35	37.64089734427996	40.0	36.0	41.0	32.0	41.0
36-37	37.55437253388831	40.0	36.0	41.0	32.0	41.0
38-39	37.441419092510074	40.0	36.0	41.0	32.0	41.0
40-41	37.312132024378656	40.0	36.0	41.0	31.0	41.0
42-43	37.1828218725185	39.0	35.0	41.0	31.0	41.0
44-45	36.97487273170887	39.0	35.0	41.0	31.0	41.0
46-47	36.72323742440429	39.0	35.0	41.0	30.0	41.0
48-49	36.44916095542574	39.0	35.0	41.0	30.0	41.0
50-51	36.24371339130474	39.0	35.0	41.0	29.0	41.0
52-53	36.05577336646944	38.0	35.0	40.5	29.0	41.0
54-55	35.85748749421508	38.0	35.0	40.0	29.0	41.0
56-57	35.59903856969299	38.0	34.0	40.0	28.0	41.0
58-59	35.31759937055568	37.0	34.0	40.0	28.0	41.0
60-61	35.0570431114516	37.0	34.0	40.0	28.0	41.0
62-63	34.78913321577886	36.0	34.0	39.5	27.0	41.0
64-65	34.51141567332019	36.0	33.5	39.0	27.0	41.0
66-67	34.291935440546666	35.0	33.0	39.0	26.5	41.0
68-69	34.10050921306589	35.0	33.0	38.5	26.0	41.0
70-71	33.893620944468665	35.0	33.0	38.0	26.0	40.5
72-73	33.6940688247668	35.0	33.0	37.0	26.0	40.0
74-75	33.50747646998583	35.0	33.0	37.0	26.0	39.5
76-77	33.34246558285996	35.0	33.0	36.0	26.0	39.0
78-79	33.228658463222445	35.0	33.0	36.0	27.0	39.0
80-81	33.08631705683298	35.0	33.0	36.0	27.0	38.0
82-83	32.8969665462209	35.0	33.0	35.0	26.0	37.0
84-85	32.750962521537815	35.0	33.0	35.0	26.0	37.0
86-87	32.608021497806625	35.0	33.0	35.0	26.0	36.5
88-89	32.4920806022251	35.0	33.0	35.0	26.0	36.0
90-91	32.399979602377876	35.0	33.0	35.0	26.0	36.0
92-93	32.320717912357935	35.0	33.0	35.0	26.0	36.0
94-95	32.275958282525814	35.0	33.0	35.0	26.0	35.0
96-97	32.22344811589207	35.0	33.0	35.0	25.5	35.0
98-99	32.18418689034473	35.0	33.0	35.0	25.0	35.0
100	31.689340857416425	34.0	32.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4827154403717202
1101	2	0.582534925497491
1101	3	0.5191594311286636
1101	4	0.3335175836205764
1101	5	0.37453264707374245
1101	6	0.408125557561938
1101	7	0.3983476646731461
1101	8	0.4014958084620375
1101	9	0.47558655579031495
1101	10-11	0.5363928999951924
1101	12-13	0.5621694997934554
1101	14-15	0.7042224248611717
1101	16-17	0.6778701920650434
1101	18-19	0.6746667723226878
1101	20-21	0.6743430217289017
1101	22-23	0.6473920369970401
1101	24-25	0.7261084823776045
1101	26-27	0.7347464282846587
1101	28-29	0.7471312983295988
1101	30-31	0.7464365481623005
1101	32-33	0.7335739691433503
1101	34-35	0.8085156097042869
1101	36-37	0.8305662740478041
1101	38-39	0.8406719021157869
1101	40-41	0.8712905153080115
1101	42-43	0.843829197507219
1101	44-45	0.7988083601290015
1101	46-47	0.7985583771623297
1101	48-49	0.8475442829220796
1101	50-51	0.844561349064719
1101	52-53	0.8569902831632135
1101	54-55	0.7796960886537434
1101	56-57	0.783029459375733
1101	58-59	0.7957997168476183
1101	60-61	0.8290935874918404
1101	62-63	0.765336138830925
1101	64-65	0.7150469468599425
1101	66-67	0.6804334389911446
1101	68-69	0.693812859311187
1101	70-71	0.6481740313904965
1101	72-73	0.6597992836972324
1101	74-75	0.6383821833666445
1101	76-77	0.6999844097257437
1101	78-79	0.6544654572216189
1101	80-81	0.632690281905397
1101	82-83	0.5924510779393586
1101	84-85	0.5705795397269071
1101	86-87	0.606669052419555
1101	88-89	0.5842059221074294
1101	90-91	0.6142972757866403
1101	92-93	0.6114729547729709
1101	94-95	0.5692361628615075
1101	96-97	0.6163178059277357
1101	98-99	0.667790474009152
1101	100	0.6040458133911955
1102	1	0.4468003525198512
1102	2	0.5459747407011548
1102	3	0.4861709333137796
1102	4	0.30398567394794185
1102	5	0.3309111972624308
1102	6	0.35160303976647356
1102	7	0.36227306181378083
1102	8	0.42491783002613914
1102	9	0.48323171052805947
1102	10-11	0.49372294901705516
1102	12-13	0.5042425950112985
1102	14-15	0.5912673015293137
1102	16-17	0.6165364452491175
1102	18-19	0.6517807406920326
1102	20-21	0.6064785828007331
1102	22-23	0.6260722588047685
1102	24-25	0.6493002470417224
1102	26-27	0.693023743540131
1102	28-29	0.7048422813123523
1102	30-31	0.7174832156903506
1102	32-33	0.7401614268604675
1102	34-35	0.7188502522636924
1102	36-37	0.7473775777570992
1102	38-39	0.7481563156114319
1102	40-41	0.7289023126324707
1102	42-43	0.7150338402822669
1102	44-45	0.722743790794965
1102	46-47	0.7150060779254162
1102	48-49	0.7057706432525777
1102	50-51	0.6315200688172595
1102	52-53	0.583596626983443
1102	54-55	0.638313589954798
1102	56-57	0.6382451002210985
1102	58-59	0.6122737816547428
1102	60-61	0.620147112179545
1102	62-63	0.6421233539974409
1102	64-65	0.5798057062769928
1102	66-67	0.6584049703522865
1102	68-69	0.6784494286300315
1102	70-71	0.5870920807034423
1102	72-73	0.5771537013450043
1102	74-75	0.6206733351468685
1102	76-77	0.516694785966223
1102	78-79	0.5336696783391659
1102	80-81	0.4969727258174359
1102	82-83	0.534583342460607
1102	84-85	0.5241976686022838
1102	86-87	0.5941484655255564
1102	88-89	0.5571471743570271
1102	90-91	0.5714773549226706
1102	92-93	0.5791745274502063
1102	94-95	0.5501024962176331
1102	96-97	0.5322093640096455
1102	98-99	0.5384091368888022
1102	100	0.5177794075827151
1103	1	0.2784544695858031
1103	2	-0.12803210087052364
1103	3	0.20410548884703417
1103	4	0.20028202871840506
1103	5	0.2601616367897819
1103	6	0.21662141308109994
1103	7	0.30365148894302507
1103	8	0.31193357463452287
1103	9	0.3946508087385965
1103	10-11	0.4257972249105464
1103	12-13	0.4375697634965974
1103	14-15	0.5346780492840253
1103	16-17	0.5693595168827699
1103	18-19	0.5419912169209056
1103	20-21	0.5171928232050007
1103	22-23	0.50014515282313
1103	24-25	0.44553984949924086
1103	26-27	0.5268300363087661
1103	28-29	0.5603518052977492
1103	30-31	0.6007855145114362
1103	32-33	0.5928756245524767
1103	34-35	0.5470040442654849
1103	36-37	0.5035182210120084
1103	38-39	0.5836947803161721
1103	40-41	0.6512562043243619
1103	42-43	0.6081421063849248
1103	44-45	0.6758648261680378
1103	46-47	0.6581303505485252
1103	48-49	0.669422538294917
1103	50-51	0.698574279821969
1103	52-53	0.6853740978658109
1103	54-55	0.6060463900287729
1103	56-57	0.5417699918757748
1103	58-59	0.5712473288180817
1103	60-61	0.5948395064674443
1103	62-63	0.5802878078501692
1103	64-65	0.5969012833180471
1103	66-67	0.6254262520467435
1103	68-69	0.5806682488303636
1103	70-71	0.4802587061144976
1103	72-73	0.5334911944941325
1103	74-75	0.4145687506143858
1103	76-77	0.4772238765308501
1103	78-79	0.4052018103541215
1103	80-81	0.4255079818879324
1103	82-83	0.4756676999950997
1103	84-85	0.42305393892935683
1103	86-87	0.43631966297962066
1103	88-89	0.40197849551591247
1103	90-91	0.4283949029464651
1103	92-93	0.4698308391926673
1103	94-95	0.4263504374309264
1103	96-97	0.4269886058291874
1103	98-99	0.43932532164478744
1103	100	0.44099825752655875
1104	1	0.26204022615266354
1104	2	0.3619752775767182
1104	3	0.27358398772248904
1104	4	0.22883951115291268
1104	5	0.2188167020599181
1104	6	0.20670478275555837
1104	7	0.18449523852795835
1104	8	0.23946146414127867
1104	9	0.2960685691439622
1104	10-11	0.28660260324932096
1104	12-13	0.2958017475227379
1104	14-15	0.37362116315755856
1104	16-17	0.35694022254070035
1104	18-19	0.3942277974996884
1104	20-21	0.4038031551341206
1104	22-23	0.3490005215601073
1104	24-25	0.3799635364593854
1104	26-27	0.41536754311580637
1104	28-29	0.40199042865354073
1104	30-31	0.39607640699225755
1104	32-33	0.41850081755706015
1104	34-35	0.4331389208421683
1104	36-37	0.4420772039014196
1104	38-39	0.3568933074491625
1104	40-41	0.3399677403806507
1104	42-43	0.3430247637549044
1104	44-45	0.3724703357499237
1104	46-47	0.4025395130497955
1104	48-49	0.4293436132598316
1104	50-51	0.47603481834208594
1104	52-53	0.4956466936514232
1104	54-55	0.3818053753255626
1104	56-57	0.3523227382962659
1104	58-59	0.43364475276736414
1104	60-61	0.3966866570835066
1104	62-63	0.3871047743495595
1104	64-65	0.31570991105138546
1104	66-67	0.2749807919570557
1104	68-69	0.2504715107598514
1104	70-71	0.29588589903384843
1104	72-73	0.3585261670611928
1104	74-75	0.29590855308286024
1104	76-77	0.2644570183077235
1104	78-79	0.2420706093039513
1104	80-81	0.2228915385623793
1104	82-83	0.2583147867743776
1104	84-85	0.262743095033116
1104	86-87	0.2804241272930099
1104	88-89	0.1951239934317357
1104	90-91	0.17371310661208383
1104	92-93	0.2175618421468073
1104	94-95	0.2119485572204951
1104	96-97	0.27471562417278506
1104	98-99	0.23530897256633665
1104	100	0.296351740906303
1105	1	0.12355321925151941
1105	2	-0.37446613678505614
1105	3	0.032544481791951796
1105	4	0.057427314072022284
1105	5	0.1020736077150346
1105	6	0.1750711555126898
1105	7	0.24130609514551793
1105	8	0.21365098537511784
1105	9	0.3017327643321295
1105	10-11	0.25607063496670435
1105	12-13	0.29094274249678165
1105	14-15	0.37135833895500525
1105	16-17	0.3236941740833572
1105	18-19	0.3452525756254232
1105	20-21	0.33326785261062497
1105	22-23	0.32056394016092327
1105	24-25	0.31874024180096683
1105	26-27	0.36328294933608163
1105	28-29	0.3760632480831134
1105	30-31	0.3931400890702932
1105	32-33	0.38985613283931997
1105	34-35	0.41861372075819503
1105	36-37	0.45080283537894417
1105	38-39	0.40894749028031896
1105	40-41	0.3972372575219225
1105	42-43	0.37644481073989766
1105	44-45	0.46236334554629366
1105	46-47	0.41816431433710477
1105	48-49	0.4713888664379766
1105	50-51	0.4890667278436851
1105	52-53	0.4566128327890553
1105	54-55	0.4353822476760527
1105	56-57	0.5265390677173727
1105	58-59	0.4102170721788738
1105	60-61	0.4361393558131539
1105	62-63	0.5127690376798313
1105	64-65	0.534860887968108
1105	66-67	0.4850961582433442
1105	68-69	0.4195901690861419
1105	70-71	0.4406156006870958
1105	72-73	0.4338715944503875
1105	74-75	0.4232163401174276
1105	76-77	0.3836723220550695
1105	78-79	0.339048627973483
1105	80-81	0.4137218262749158
1105	82-83	0.34564705302019405
1105	84-85	0.32288328427726753
1105	86-87	0.3648102928380652
1105	88-89	0.34854287727807787
1105	90-91	0.39728851390711384
1105	92-93	0.36392412091221615
1105	94-95	0.3758804536975049
1105	96-97	0.31849600497183417
1105	98-99	0.3482597895784849
1105	100	0.3353910666313098
1106	1	0.24439261778693577
1106	2	0.2827692768921537
1106	3	0.2410155140598249
1106	4	0.15930230623789754
1106	5	0.16150263081738814
1106	6	0.191588555989334
1106	7	0.18407290908464802
1106	8	0.24927152435199673
1106	9	0.25234719132925676
1106	10-11	0.2181515808221377
1106	12-13	0.2645492356373609
1106	14-15	0.31957753340533657
1106	16-17	0.32487397623832237
1106	18-19	0.3034942724069225
1106	20-21	0.3312960034138541
1106	22-23	0.290507128450777
1106	24-25	0.3155598766789893
1106	26-27	0.27623436182196315
1106	28-29	0.30876343313195065
1106	30-31	0.2812466649119827
1106	32-33	0.25931216119148814
1106	34-35	0.29376471499718804
1106	36-37	0.2837917283612583
1106	38-39	0.23508215425223256
1106	40-41	0.2991058529546464
1106	42-43	0.35994086452095786
1106	44-45	0.3243450739149836
1106	46-47	0.3393573282325235
1106	48-49	0.28814085467071493
1106	50-51	0.2953272391954016
1106	52-53	0.30482583146855546
1106	54-55	0.30069448387041575
1106	56-57	0.36696942548209677
1106	58-59	0.2994677708465474
1106	60-61	0.2805854518576112
1106	62-63	0.2620705062444202
1106	64-65	0.2443955389149295
1106	66-67	0.26498954329915847
1106	68-69	0.21276353663166958
1106	70-71	0.2665931734272817
1106	72-73	0.31484932114815933
1106	74-75	0.2558441310413997
1106	76-77	0.20707376208981998
1106	78-79	0.25330816898369335
1106	80-81	0.31693932174202644
1106	82-83	0.2744367325213801
1106	84-85	0.2924123539211365
1106	86-87	0.28820346957429166
1106	88-89	0.21553657210199617
1106	90-91	0.12684475229558245
1106	92-93	0.2733211228812422
1106	94-95	0.26527105961460506
1106	96-97	0.17613057752291184
1106	98-99	0.1884379566494374
1106	100	0.2149029093754926
1107	1	-0.0014551723320508358
1107	2	-0.4867723537181803
1107	3	-0.062310158615716205
1107	4	0.01090666692142861
1107	5	0.031937577063082756
1107	6	0.10535566779072525
1107	7	0.14474577095550245
1107	8	0.14840369248347685
1107	9	0.18859112249349153
1107	10-11	0.23262726807548972
1107	12-13	0.22275010361994418
1107	14-15	0.21006258676505496
1107	16-17	0.24771308639451206
1107	18-19	0.25606052198013884
1107	20-21	0.20710514726341245
1107	22-23	0.1984551728045858
1107	24-25	0.19424777849359032
1107	26-27	0.3120541766563605
1107	28-29	0.21588292966588796
1107	30-31	0.24585849850462438
1107	32-33	0.24455603261958458
1107	34-35	0.2820729751354847
1107	36-37	0.2877464047968914
1107	38-39	0.2977241132195303
1107	40-41	0.20485489484721597
1107	42-43	0.1864837216403359
1107	44-45	0.18482850645232674
1107	46-47	0.20336116847177976
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2216	76-77	-0.7500296440621526
2216	78-79	-0.7312775397951867
2216	80-81	-0.6170744005568451
2216	82-83	-0.6650061510751044
2216	84-85	-0.6666093877847032
2216	86-87	-0.6276436190164638
2216	88-89	-0.559754063053262
2216	90-91	-0.636139966177673
2216	92-93	-0.6292561254232929
2216	94-95	-0.6274684609342174
2216	96-97	-0.7008675534084858
2216	98-99	-0.6831256591189074
2216	100	-0.5932177426022918
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	11.0
13	31.0
14	211.0
15	723.0
16	1645.0
17	3312.0
18	5616.0
19	9062.0
20	13493.0
21	18918.0
22	26215.0
23	31076.0
24	32980.0
25	34108.0
26	36865.0
27	43899.0
28	54572.0
29	68239.0
30	84811.0
31	104833.0
32	130036.0
33	165729.0
34	225455.0
35	311033.0
36	458794.0
37	773545.0
38	672607.0
39	265627.0
40	498.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.975625898112895	21.690040338998703	14.074182900909902	24.2601508619785
2	14.851786851539494	20.241974026322886	40.252288058667425	24.653951063470195
3	17.91839390017838	26.568528557937814	29.947773774964148	25.565303766919662
4	12.291134482510587	17.576987869629797	38.71138921440732	31.42048843345229
5	12.713653061983976	38.53862634704435	34.99000935407627	13.757711236895409
6	29.465442398961823	38.859400785574266	17.78703427528004	13.888122540183875
7	25.9138953958833	32.61466642342384	23.744156296202615	17.72728188449025
8	26.200367884685445	34.886089493238	19.701444845557265	19.212097776519286
9	26.028121297859563	14.641043821031433	21.66244257747599	37.66839230363301
10-11	25.57205676862493	26.107557305006846	27.188071672039815	21.13231425432841
12-13	26.072218214824733	23.903508894650336	27.750363323900253	22.273909566624678
14-15	23.359420602936048	24.750576861838024	25.717586464915666	26.172416070310266
16-17	21.667417023120958	27.26344394865267	27.126393137722076	23.942745890504295
18-19	21.171836395961215	27.003289921000484	29.86234263192561	21.96253105111269
20-21	22.6613836165101	26.12514656421034	29.139728068041325	22.07374175123823
22-23	23.060008881002126	25.796792934147117	28.868405317297164	22.274792867553593
24-25	22.493893653386714	25.972360238845276	29.609321127028938	21.924424980739072
26-27	22.38717414436796	26.173250955458926	28.71586712356709	22.723707776606023
28-29	21.633580921480068	26.736316105503555	29.165871560454466	22.464231412561915
30-31	22.51294507527534	26.190085132791594	28.98730143096734	22.309668360965723
32-33	22.99637431567237	26.542021060195093	28.165730539857066	22.29587408427548
34-35	22.896919791809044	26.37195938757063	28.933369017668365	21.797751802951957
36-37	22.697181210908056	25.986640536818406	28.755862567593354	22.560315684680184
38-39	22.82078408571366	25.86249204660619	29.371078913895172	21.945644953784978
40-41	22.348867564743063	25.270241344624345	29.74431543942461	22.636575651207984
42-43	22.516581214373893	25.510363398885932	28.879335430747826	23.09371995599235
44-45	22.620039586496272	26.494132815660898	28.463426699787853	22.422400898054978
46-47	22.761102154313466	26.207894579268963	27.806435799533624	23.224567466883943
48-49	23.661599415769192	25.92215012605171	27.681292023067044	22.73495843511205
50-51	23.11824564756234	26.100272094244303	27.270743216425657	23.510739041767707
52-53	22.39097904668957	26.74188418067872	27.22636268147308	23.640774091158622
54-55	22.54767257336553	26.70276878170611	27.00629963626942	23.74325900865894
56-57	23.516684021445776	26.072166501792765	26.894213632644163	23.516935844117288
58-59	22.527273129172045	26.398645863849385	27.73130710960652	23.34277389737206
60-61	22.570485155895	26.988699319295435	27.035748181840567	23.405067342968998
62-63	23.046235169753544	26.2447725847005	26.620701599856293	24.088290645689668
64-65	22.159795105997	26.446723935773502	27.031092108147703	24.362388850081793
66-67	22.831388610796022	25.99353767516353	27.099598035336854	24.075475678703594
68-69	22.387610967617196	25.90659287619166	27.39202770752312	24.313768448668025
70-71	22.449861832540368	25.591422761088374	27.54266738744305	24.416048018928215
72-73	23.00970691778779	25.70611027698796	27.781743204849768	23.50243960037449
74-75	23.071596053655067	26.235079492101153	27.276241347084728	23.417083107159044
76-77	22.772554267418705	26.182711793505746	26.849191453760334	24.195542485315215
78-79	22.397287804001785	26.220249666298773	27.161272442203337	24.221190087496105
80-81	22.118274065442428	26.5962681354122	27.64768518822942	23.63777261091595
82-83	22.97113021726311	25.516492172050505	28.61804041391844	22.89433719676795
84-85	23.32541496578462	26.117517453963384	27.07226859341503	23.484798986836967
86-87	22.03951064101572	26.730970783604814	27.541756145704078	23.687762429675388
88-89	22.792285633696878	25.61637867350776	27.35446719586669	24.236868496928672
90-91	22.4769867569358	25.455084739241542	27.776129186157704	24.29179931766495
92-93	22.77194933376464	25.955364553223	27.2784471502901	23.99423896272227
94-95	21.505008534094408	25.808962198158873	28.000699515934972	24.685329751811746
96-97	21.85239225757421	25.743024316345437	27.626235400825934	24.778348025254427
98-99	21.27078466713404	26.50073302715803	27.476004010768694	24.752478294939237
100	22.098027075382113	25.753480894529897	26.709909540567566	25.43858248952042
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	197.0
1	146.5
2	142.5
3	186.0
4	129.5
5	99.0
6	221.0
7	564.0
8	992.5
9	1203.0
10	971.0
11	657.5
12	616.0
13	701.0
14	861.5
15	1180.5
16	1567.5
17	1999.5
18	2588.0
19	3356.0
20	4237.5
21	5150.0
22	6160.5
23	7215.0
24	8193.5
25	9258.0
26	10807.5
27	12515.0
28	16000.0
29	23451.0
30	33066.0
31	41795.5
32	54934.0
33	66298.5
34	72207.5
35	81052.5
36	89693.5
37	107169.0
38	115088.0
39	103924.0
40	94731.5
41	86690.0
42	84402.0
43	90994.5
44	99995.0
45	120540.0
46	140735.5
47	154146.0
48	164177.0
49	160687.5
50	153043.5
51	155483.0
52	157024.5
53	152323.0
54	148223.0
55	130558.0
56	114285.5
57	97374.0
58	79505.0
59	75931.0
60	63730.5
61	45383.0
62	34126.5
63	27789.0
64	20145.0
65	10677.5
66	6460.5
67	4637.5
68	3477.5
69	2490.0
70	1768.0
71	1435.0
72	1164.0
73	929.0
74	742.0
75	583.0
76	433.0
77	273.0
78	150.0
79	83.0
80	50.0
81	31.0
82	18.5
83	6.0
84	4.0
85	3.5
86	3.5
87	3.5
88	1.0
89	0.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013458513363100619
2	0.00486856824361644
3	0.0019865996856136044
4	0.028344020866571572
5	0.002881968558002835
6	0.0
7	3.917238816702882E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008394083178649033
16-17	0.002490244676332547
18-19	0.0
20-21	1.3990138631081724E-5
22-23	6.995069315540862E-4
24-25	1.3990138631081724E-5
26-27	2.51822495359471E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	4.336942975635335E-4
36-37	0.0
38-39	0.0
40-41	3.077830498837979E-4
42-43	6.155660997675958E-4
44-45	0.0
46-47	0.0
48-49	4.4768443619461515E-4
50-51	1.3990138631081724E-5
52-53	0.0
54-55	2.6581263399055275E-4
56-57	6.995069315540862E-5
58-59	1.958619408351441E-4
60-61	5.5960554524326894E-5
62-63	0.0
64-65	1.3990138631081724E-5
66-67	0.0
68-69	0.001203151922273028
70-71	6.155660997675958E-4
72-73	0.0
74-75	1.6788166357298068E-4
76-77	0.0026161559240122823
78-79	4.336942975635335E-4
80-81	0.0028679784193717532
82-83	0.0014969448335257444
84-85	0.005246301986655646
86-87	0.007009059454171943
88-89	0.006995069315540862
90-91	0.005274282263917809
92-93	0.007946398742454418
94-95	0.0012870927540595185
96-97	0.004378913391528579
98-99	0.0018886687151960326
100	0.08262575875516866
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3573946.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.379656086199333
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.52772447781213	17.392100796166957
2	15.519285053072682	7.877482347013569
3	6.150232911520455	4.682723884332407
4	3.030446647848971	3.0764677483993
5	1.6615951833749472	2.108535715427074
6	1.0270671699928706	1.563996693110705
7	0.6642813972126677	1.1801463384002233
8	0.46574891515887135	0.9456437831402068
9	0.3584037531384165	0.8186547595190486
>10	1.9538168677842547	9.343625025514656
>50	0.24758629275666322	4.437498859925696
>100	0.2977423524472304	16.61936300016791
>500	0.060373623713550935	10.794448895204004
>1k	0.034152962319709435	15.804357821929727
>5k	9.915376157334998E-4	1.7705173330498123
>10k+	5.508542309630555E-4	1.5844369986987044
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	12806	0.3583154306192651	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	12644	0.3537826257027946	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	10620	0.29715054452417583	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	10357	0.28979173160422683	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	10239	0.2864900588872915	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	9508	0.26603647620865006	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	8781	0.2456948146390572	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8431	0.23590171759730003	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8278	0.23162073517618903	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6488	0.18153603887691644	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6289	0.17596796370174592	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	5340	0.1494146805799528	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	5168	0.1446020728908607	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5038	0.14096463684677943	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	4982	0.1393977413200983	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	4980	0.13934178076557396	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	4867	0.13618000943494948	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	4865	0.13612404888042517	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	4860	0.13598414749411436	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	4702	0.13156326368669252	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4654	0.13022021037810866	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4513	0.12627499128414363	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	4499	0.12588326740247335	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4493	0.12571538573890034	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4490	0.12563144490711387	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	4225	0.11821667143264057	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4190	0.11723736172846483	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	4152	0.11617411119250264	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	4098	0.1146631762203458	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	3967	0.11099775989900239	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	3940	0.11024229241292398	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	3934	0.11007441074935101	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	3868	0.10822771245004821	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	3866	0.10817175189552389	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	3864	0.10811579134099955	No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC	3788	0.10598929026907514	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	3728	0.10431047363334532	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	3651	0.10215599228415874	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.5960554524326894E-5
2	0.0	0.0	0.0	0.0	5.5960554524326894E-5
3	0.0	0.0	0.0	0.0	5.5960554524326894E-5
4	0.0	0.0	0.0	0.0	5.5960554524326894E-5
5	0.0	0.0	0.0	0.0	5.5960554524326894E-5
6	0.0	0.0	0.0	0.0	1.1192110904865379E-4
7	0.0	0.0	0.0	0.0	1.1192110904865379E-4
8	0.0	0.0	0.0	0.0	1.6788166357298068E-4
9	0.0	0.0	0.0	2.7980277262163447E-5	1.958619408351441E-4
10-11	0.0	0.0	0.0	2.7980277262163447E-5	1.958619408351441E-4
12-13	0.0	0.0	0.0	2.7980277262163447E-5	3.7773374303920655E-4
14-15	0.0	0.0	0.0	2.7980277262163447E-5	4.6167457482569683E-4
16-17	0.0	0.0	0.0	4.197041589324517E-5	5.03644990718942E-4
18-19	0.0	0.0	0.0	5.5960554524326894E-5	6.155660997675958E-4
20-21	0.0	0.0	0.0	1.1192110904865379E-4	7.414773474473314E-4
22-23	0.0	0.0	0.0	1.958619408351441E-4	7.694576247094947E-4
24-25	0.0	0.0	0.0	3.7773374303920655E-4	7.834477633405765E-4
26-27	0.0	0.0	0.0	5.176351293500238E-4	8.673885951270668E-4
28-29	0.0	0.0	0.0	0.0013430533085838454	8.673885951270668E-4
30-31	0.0	0.0	0.0	0.005260292125286728	8.673885951270668E-4
32-33	0.0	0.0	0.0	0.015710925682704775	8.673885951270668E-4
34-35	0.0	0.0	0.0	0.03144983164267171	9.09359011020312E-4
36-37	0.0	0.0	0.0	0.05963996098430139	9.513294269135571E-4
38-39	0.0	0.0	0.0	0.104254513078821	0.001007289981437884
40-41	0.0	0.0	0.0	0.1544791107644044	0.001007289981437884
42-43	0.0	0.0	0.0	0.20836912477133118	0.0010352702587000475
44-45	0.0	0.0	0.0	0.2742906580009883	0.001063250535962211
46-47	0.0	0.0	0.0	0.35935070087796517	0.001105220951855456
48-49	0.0	0.0	0.0	0.46115693969634686	0.0011751716450108648
50-51	0.0	0.0	0.0	0.5743651414990602	0.0012311321995351916
52-53	0.0	0.0	0.0	0.6850271380709165	0.0013710335858460089
54-55	0.0	0.0	0.0	0.8093714902239709	0.0014409842790014176
56-57	0.0	0.0	0.0	0.9654734570695809	0.0014689645562635809
58-59	0.0	0.0	0.0	1.1520599359923178	0.0014829546948946627
60-61	0.0	0.0	0.0	1.3491949794428901	0.0014829546948946627
62-63	2.7980277262163447E-5	0.0	0.0	1.5568785874212985	0.0015389152494189894
64-65	2.7980277262163447E-5	0.0	0.0	1.777139330029049	0.0015808856653122347
66-67	2.7980277262163447E-5	0.0	0.0	2.0289058648339955	0.0016368462198365617
68-69	2.7980277262163447E-5	0.0	0.0	2.320824097510147	0.0016788166357298068
70-71	2.7980277262163447E-5	0.0	0.0	2.6416739368753754	0.0017347771902541337
72-73	2.7980277262163447E-5	0.0	0.0	2.9785424849731923	0.0019446292697203596
74-75	2.7980277262163447E-5	0.0	0.0	3.314277831841891	0.0020285701015068497
76-77	2.7980277262163447E-5	0.0	0.0	3.7037632913312066	0.0020985207946622583
78-79	2.7980277262163447E-5	0.0	0.0	4.126825083535118	0.0021544813491865857
80-81	2.7980277262163447E-5	2.7980277262163447E-5	0.0	4.590919952343992	0.0022524123196041575
82-83	2.7980277262163447E-5	2.7980277262163447E-5	0.0	5.077119240189974	0.002406303844546056
84-85	2.7980277262163447E-5	2.7980277262163447E-5	0.0	5.548824744414157	0.002406303844546056
86-87	2.7980277262163447E-5	2.7980277262163447E-5	0.0	6.0550579107798495	0.0024902446763325464
88	2.7980277262163447E-5	2.7980277262163447E-5	0.0	6.459666710129364	0.0025462052308568737
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7980	0.0	47.433323	1
GTATCAA	13560	0.0	38.767933	1
GTGGTAT	2120	0.0	37.26181	1
GGGCACC	3540	0.0	34.54573	94
CAACGCA	16155	0.0	31.185555	5
ATCAACG	16280	0.0	31.014692	3
TATTCTC	2375	0.0	30.473532	5
GTACTAG	3600	0.0	30.432943	1
TATCAAC	17530	0.0	29.90271	2
TAGGCAT	4340	0.0	29.562325	5
AACGCAG	18440	0.0	28.238688	6
GGACCGT	170	4.08545E-9	27.645008	6
TAGTACT	4895	0.0	27.083694	4
TCAACGC	19155	0.0	26.653805	4
CTGTGCG	4905	0.0	26.640623	9
CTAGGCA	5100	0.0	26.548122	4
ACGCAGA	19090	0.0	26.317066	7
CGCAGAG	19345	0.0	25.969797	8
CTAACGC	1760	0.0	25.910553	3
TCTAACG	1785	0.0	25.547659	2
>>END_MODULE