##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146483_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4996006
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.66173839663123	31.0	31.0	34.0	27.0	34.0
2	30.648698380266158	31.0	31.0	34.0	27.0	34.0
3	30.894514338053238	31.0	31.0	34.0	27.0	34.0
4	34.36584223477714	37.0	35.0	37.0	30.0	37.0
5	34.578711274566125	37.0	35.0	37.0	30.0	37.0
6	34.730038554797574	37.0	35.0	37.0	31.0	37.0
7	34.5961075707275	37.0	35.0	37.0	30.0	37.0
8	34.53851136287667	37.0	35.0	37.0	30.0	37.0
9	35.93070925054934	39.0	35.0	39.0	30.0	39.0
10-11	35.855685621674596	39.0	35.0	39.0	30.0	39.0
12-13	35.851295414777326	39.0	35.0	39.0	30.0	39.0
14-15	36.88003126897766	40.0	36.0	41.0	30.0	41.0
16-17	36.715786670392305	40.0	36.0	41.0	29.5	41.0
18-19	36.670086465068295	40.0	36.0	41.0	28.5	41.0
20-21	37.01036317810667	40.0	36.0	41.0	30.0	41.0
22-23	36.71078787735644	40.0	36.0	41.0	29.5	41.0
24-25	36.82743585576158	40.0	36.0	41.0	30.0	41.0
26-27	36.90398500322057	40.0	36.0	41.0	30.0	41.0
28-29	36.730605107359764	40.0	36.0	41.0	29.5	41.0
30-31	36.67415391414662	40.0	35.5	41.0	30.0	41.0
32-33	36.781836831260804	40.0	35.5	41.0	30.0	41.0
34-35	36.840166224780354	40.0	35.0	41.0	30.0	41.0
36-37	36.77718811786855	40.0	35.0	41.0	30.0	41.0
38-39	36.59888428876987	39.0	35.0	41.0	30.0	41.0
40-41	36.55857358858256	39.0	35.0	41.0	30.0	41.0
42-43	36.39831897719898	39.0	35.0	41.0	29.0	41.0
44-45	36.20127307693385	39.0	35.0	41.0	28.5	41.0
46-47	36.070175956554095	39.0	35.0	41.0	28.0	41.0
48-49	35.93250888809982	38.0	34.5	40.0	28.0	41.0
50-51	35.70143910956072	38.0	34.0	40.0	27.5	41.0
52-53	35.502948255066144	38.0	34.0	40.0	27.5	41.0
54-55	35.30325313860712	37.5	34.0	40.0	27.0	41.0
56-57	35.1477638337504	37.0	33.5	40.0	27.0	41.0
58-59	34.86358803412166	36.5	33.0	40.0	27.0	41.0
60-61	34.605877575006915	36.0	33.0	39.5	26.5	41.0
62-63	34.334186247974884	35.5	33.0	39.0	26.0	41.0
64-65	34.04741007116485	35.0	33.0	39.0	26.0	41.0
66-67	33.75952811105511	35.0	32.0	38.5	26.0	40.5
68-69	33.47357359058415	35.0	32.0	38.0	26.0	40.0
70-71	33.43582143416161	35.0	32.0	37.5	26.0	40.0
72-73	33.239723891444484	35.0	32.0	37.0	26.0	40.0
74-75	32.97636281861951	35.0	32.0	36.5	25.0	39.0
76-77	32.75778992259016	35.0	32.0	36.0	25.0	39.0
78-79	32.5510508794425	35.0	32.0	36.0	25.0	39.0
80-81	32.35140940583338	35.0	31.5	35.5	24.5	38.0
82-83	32.11827507813241	35.0	31.0	35.0	24.0	37.0
84-85	31.949301902359608	35.0	31.0	35.0	24.0	37.0
86-87	31.79141157956976	35.0	31.0	35.0	24.0	36.0
88-89	31.80950703421893	35.0	32.0	35.0	24.0	36.0
90-91	31.744591279514076	35.0	32.0	35.0	24.0	36.0
92-93	31.59965330305848	35.0	32.0	35.0	24.0	36.0
94-95	31.595936934423214	35.0	32.0	35.0	24.0	35.0
96-97	31.519980060071987	35.0	32.0	35.0	24.0	35.0
98-99	31.45482581486091	35.0	31.0	35.0	24.0	35.0
100	30.971746030729346	34.0	31.0	35.0	23.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8098639140402071
1101	2	0.9496983902892069
1101	3	0.9318689797240758
1101	4	0.892468724563912
1101	5	0.8876042665144013
1101	6	0.6061555485460488
1101	7	0.6924252707435627
1101	8	0.7067331902481513
1101	9	0.9015972409359918
1101	10-11	0.9766907329814742
1101	12-13	0.8125136904717678
1101	14-15	1.0805070524770244
1101	16-17	1.2029278077719567
1101	18-19	1.3388396926972987
1101	20-21	0.9935371536320758
1101	22-23	1.3201639456970256
1101	24-25	1.1532801331367182
1101	26-27	1.1000500794883266
1101	28-29	1.3607478515653284
1101	30-31	1.3104093163247796
1101	32-33	1.1602260993327391
1101	34-35	1.0471376888283572
1101	36-37	0.9768208936017473
1101	38-39	1.035136694151987
1101	40-41	0.9858016790202697
1101	42-43	0.9910942227950912
1101	44-45	1.0629274513731417
1101	46-47	1.0586902966726441
1101	48-49	1.0217540877051405
1101	50-51	1.0069987665412796
1101	52-53	0.9948890657997609
1101	54-55	1.0239713358320444
1101	56-57	0.9463765290840129
1101	58-59	0.873910582355677
1101	60-61	0.8938368144815172
1101	62-63	0.8655550685600488
1101	64-65	0.8379653572012273
1101	66-67	0.8130847363460418
1101	68-69	0.8664839341750294
1101	70-71	0.7678215654655958
1101	72-73	0.7674145108439205
1101	74-75	0.7677080203327122
1101	76-77	0.7665823309323372
1101	78-79	0.7656832795076269
1101	80-81	0.8157065536381864
1101	82-83	0.7366169655225505
1101	84-85	0.7106433385909661
1101	86-87	0.7762522932377607
1101	88-89	0.762918245541254
1101	90-91	0.7770358257890706
1101	92-93	0.8204839620329665
1101	94-95	0.8316672898834021
1101	96-97	0.7949766078398781
1101	98-99	0.7779141263774356
1101	100	0.7932035988126138
1102	1	0.7342630548525868
1102	2	0.8133343675030247
1102	3	0.8226297712353592
1102	4	0.8848063861101636
1102	5	0.6980145699045863
1102	6	0.5331667689778072
1102	7	0.6292888161405941
1102	8	0.6441481537779694
1102	9	0.8636258638215821
1102	10-11	0.7743376005811626
1102	12-13	0.7263955244134692
1102	14-15	0.8839685056073137
1102	16-17	1.0721395819014035
1102	18-19	1.0976514176546388
1102	20-21	0.8482088715732203
1102	22-23	1.1658139077684595
1102	24-25	1.059214835208806
1102	26-27	0.9054037856884989
1102	28-29	1.0840710104272375
1102	30-31	1.1457605225420693
1102	32-33	0.9674488305042317
1102	34-35	0.8161301671374517
1102	36-37	0.8115228979741218
1102	38-39	0.9221553543462377
1102	40-41	0.8354968849889985
1102	42-43	0.8354964315972992
1102	44-45	0.8957011474003451
1102	46-47	0.8166527760146849
1102	48-49	0.7583069746813109
1102	50-51	0.864605876664136
1102	52-53	0.8168875384253269
1102	54-55	0.8483454128677721
1102	56-57	0.7200807566581346
1102	58-59	0.7217323810478504
1102	60-61	0.7217821881627486
1102	62-63	0.7069702191183751
1102	64-65	0.6321764034542241
1102	66-67	0.7328381257864223
1102	68-69	0.7246797966619738
1102	70-71	0.678497752530518
1102	72-73	0.6275111841212819
1102	74-75	0.6507093855806687
1102	76-77	0.609317345310437
1102	78-79	0.5798637383985579
1102	80-81	0.6710740236622783
1102	82-83	0.683236161855163
1102	84-85	0.65209874993295
1102	86-87	0.6609974962310332
1102	88-89	0.5833658899336989
1102	90-91	0.6495063422789116
1102	92-93	0.6777717469270073
1102	94-95	0.6561167124761305
1102	96-97	0.6340784090538492
1102	98-99	0.6504993727468751
1102	100	0.7690017256836974
1103	1	0.5564188391362244
1103	2	0.7841534089860929
1103	3	0.6984934726603811
1103	4	0.7169814778917782
1103	5	0.6843713648796594
1103	6	0.4581837083130438
1103	7	0.5296338270618506
1103	8	0.5177915313120067
1103	9	0.6423229105130446
1103	10-11	0.7285125185379115
1103	12-13	0.5913778204827551
1103	14-15	0.8091323518047631
1103	16-17	1.0140730136307141
1103	18-19	1.089344656024629
1103	20-21	0.7861974547591402
1103	22-23	1.0012049879434244
1103	24-25	0.8651342771798198
1103	26-27	0.8051128794005322
1103	28-29	0.9881888724559857
1103	30-31	1.031518873527439
1103	32-33	0.8412664988951519
1103	34-35	0.7317277158706403
1103	36-37	0.7292531637445094
1103	38-39	0.8359461350716941
1103	40-41	0.7937663908633183
1103	42-43	0.8152972862703223
1103	44-45	0.8437390535738771
1103	46-47	0.8009571876465813
1103	48-49	0.7833260320045241
1103	50-51	0.7996876903913517
1103	52-53	0.8489574398498902
1103	54-55	0.846982956340895
1103	56-57	0.7340648499288491
1103	58-59	0.7396753392275812
1103	60-61	0.6630704833132768
1103	62-63	0.6331798013199403
1103	64-65	0.6949613244616728
1103	66-67	0.7357912570699199
1103	68-69	0.7072865376822648
1103	70-71	0.5987266958833146
1103	72-73	0.614483748922126
1103	74-75	0.6206893456499287
1103	76-77	0.6431832055116047
1103	78-79	0.6439834943727192
1103	80-81	0.6113984789528644
1103	82-83	0.5942972142565282
1103	84-85	0.5826070619301582
1103	86-87	0.6439223100701241
1103	88-89	0.5898859212938099
1103	90-91	0.6271828495307119
1103	92-93	0.7155233749543797
1103	94-95	0.6620437894374511
1103	96-97	0.7001779629679348
1103	98-99	0.7000453704202663
1103	100	0.7346060761004409
1104	1	0.5137869022049877
1104	2	0.7192547027371745
1104	3	0.6819030784445488
1104	4	0.7172080853703662
1104	5	0.6232582385547332
1104	6	0.3369415086996099
1104	7	0.3266302649671289
1104	8	0.4218173144694717
1104	9	0.5392186413072437
1104	10-11	0.5293343193013698
1104	12-13	0.4574585855112687
1104	14-15	0.5861040450198658
1104	16-17	0.741490764864416
1104	18-19	0.6717309149939581
1104	20-21	0.5418948878431564
1104	22-23	0.7676105870908927
1104	24-25	0.7113147807216222
1104	26-27	0.5468750887110474
1104	28-29	0.7891932219874036
1104	30-31	0.8088749096089529
1104	32-33	0.6304124811535772
1104	34-35	0.5177892414662892
1104	36-37	0.5732906012982326
1104	38-39	0.6797496711752373
1104	40-41	0.5294412214818607
1104	42-43	0.5158097966197843
1104	44-45	0.5949348312645881
1104	46-47	0.522929004711763
1104	48-49	0.47917162343095754
1104	50-51	0.582911141184745
1104	52-53	0.6417718214733981
1104	54-55	0.6499672505553917
1104	56-57	0.5591128617812586
1104	58-59	0.5436617609574199
1104	60-61	0.5605200008773323
1104	62-63	0.5154787338432953
1104	64-65	0.49537735143864836
1104	66-67	0.5364904479924064
1104	68-69	0.5040292997554374
1104	70-71	0.4435634607888801
1104	72-73	0.43456983062279164
1104	74-75	0.4582373167393641
1104	76-77	0.5204014054780615
1104	78-79	0.486971085705207
1104	80-81	0.5336576125837666
1104	82-83	0.44566583045291
1104	84-85	0.41832880813712947
1104	86-87	0.3926238530339248
1104	88-89	0.3083939951739545
1104	90-91	0.3581714638579001
1104	92-93	0.3918658664168788
1104	94-95	0.3665763102125261
1104	96-97	0.38970292472532364
1104	98-99	0.5185769862224774
1104	100	0.5755591784130232
1105	1	0.4428735712931626
1105	2	0.6003845325142052
1105	3	0.6039525070077296
1105	4	0.6777789763359792
1105	5	0.585516084423034
1105	6	0.3206891544900614
1105	7	0.4299059002621135
1105	8	0.472628769359261
1105	9	0.4940482034205971
1105	10-11	0.5779151241738703
1105	12-13	0.5424361496243577
1105	14-15	0.7282279643479583
1105	16-17	0.7793962817610094
1105	18-19	0.8416782415757211
1105	20-21	0.6326908624532308
1105	22-23	0.8998141869810539
1105	24-25	0.7944327578001307
1105	26-27	0.6350613040562862
1105	28-29	0.8806353701992862
1105	30-31	0.838517873511158
1105	32-33	0.7085374058017493
1105	34-35	0.5939700627213682
1105	36-37	0.5601964540936208
1105	38-39	0.6601079698639154
1105	40-41	0.5753198180399366
1105	42-43	0.5948537165405625
1105	44-45	0.6110811425178042
1105	46-47	0.5606420752216437
1105	48-49	0.6455763918938757
1105	50-51	0.615750394250945
1105	52-53	0.6612925492457293
1105	54-55	0.5774058768621089
1105	56-57	0.5204144863894129
1105	58-59	0.5511880501402828
1105	60-61	0.5911815118306265
1105	62-63	0.5776257509312899
1105	64-65	0.5347149462229552
1105	66-67	0.6284892037830048
1105	68-69	0.5356434762362241
1105	70-71	0.5022706778114454
1105	72-73	0.489868655095826
1105	74-75	0.4897570784706815
1105	76-77	0.5525142057592518
1105	78-79	0.46845171166232547
1105	80-81	0.5948639155995679
1105	82-83	0.5636363566265672
1105	84-85	0.5470489432151417
1105	86-87	0.5652201043586302
1105	88-89	0.5987891364950251
1105	90-91	0.5449303207424379
1105	92-93	0.5855855723607561
1105	94-95	0.46513211662439247
1105	96-97	0.5524905869934074
1105	98-99	0.594772484703352
1105	100	0.6651816564026021
1106	1	0.5606954320124018
1106	2	0.6458337389873918
1106	3	0.6185827771241712
1106	4	0.7072258539414307
1106	5	0.5273665139818391
1106	6	0.36040358149135443
1106	7	0.3488169073141023
1106	8	0.4151311198997618
1106	9	0.5664062359437878
1106	10-11	0.5224791555563186
1106	12-13	0.46513768601801786
1106	14-15	0.654219182319018
1106	16-17	0.7751891408305198
1106	18-19	0.6986731152104397
1106	20-21	0.5809224286850565
1106	22-23	0.8853758628146906
1106	24-25	0.7216331349115706
1106	26-27	0.571904850743671
1106	28-29	0.8519893634177862
1106	30-31	0.7600487761371681
1106	32-33	0.6561695530714573
1106	34-35	0.6308535378218068
1106	36-37	0.5228022400634629
1106	38-39	0.6562464194455799
1106	40-41	0.6116621012474042
1106	42-43	0.5669388138208049
1106	44-45	0.5737862970711234
1106	46-47	0.5947194069693822
1106	48-49	0.6162482441115529
1106	50-51	0.6910852106498524
1106	52-53	0.5958056423803555
1106	54-55	0.6359854710974702
1106	56-57	0.5562777289854566
1106	58-59	0.5759547937913965
1106	60-61	0.5595007987234766
1106	62-63	0.4848439310654271
1106	64-65	0.501028736602315
1106	66-67	0.5795521609257577
1106	68-69	0.5320842603999338
1106	70-71	0.5179615783173048
1106	72-73	0.4306587947219853
1106	74-75	0.49468211732351364
1106	76-77	0.5029930286575208
1106	78-79	0.524085387117097
1106	80-81	0.5557623914408509
1106	82-83	0.5523557959938756
1106	84-85	0.49593470611347
1106	86-87	0.5140396573722086
1106	88-89	0.5824359400349941
1106	90-91	0.4962294832169576
1106	92-93	0.602672399430233
1106	94-95	0.5365925438005341
1106	96-97	0.5345937326044279
1106	98-99	0.5400782818204597
1106	100	0.5221750965350758
1107	1	0.3836225626724641
1107	2	0.603418261190857
1107	3	0.45075286500863143
1107	4	0.6492263305012074
1107	5	0.5054370539003443
1107	6	0.29576831195074504
1107	7	0.2911020638025903
1107	8	0.34194533519663395
1107	9	0.45835071847347564
1107	10-11	0.3928459286639665
1107	12-13	0.34917530563330246
1107	14-15	0.5178334678788019
1107	16-17	0.604001191722638
1107	18-19	0.7073745089908101
1107	20-21	0.4608348930957078
1107	22-23	0.7928238188930479
1107	24-25	0.4996558182501474
1107	26-27	0.3918009338478825
1107	28-29	0.5983341917498564
1107	30-31	0.6097075614260561
1107	32-33	0.5322624745538675
1107	34-35	0.3829419668735312
1107	36-37	0.3652653563946373
1107	38-39	0.3992375542474633
1107	40-41	0.2505748471981377
1107	42-43	0.37892600182851766
1107	44-45	0.4546255498979406
1107	46-47	0.36011121516487776
1107	48-49	0.37489943514469104
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2216	98-99	-1.1011110326304134
2216	100	-0.9717615607116308
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	20330.0
1	3570.0
2	544.0
3	509.0
4	459.0
5	311.0
6	270.0
7	331.0
8	561.0
9	977.0
10	1625.0
11	2829.0
12	4678.0
13	6651.0
14	9044.0
15	11682.0
16	14756.0
17	17573.0
18	20951.0
19	23656.0
20	27500.0
21	32305.0
22	37090.0
23	43140.0
24	49546.0
25	56133.0
26	64307.0
27	73859.0
28	87810.0
29	105321.0
30	126265.0
31	151754.0
32	187621.0
33	240510.0
34	334622.0
35	454301.0
36	672979.0
37	1021615.0
38	796686.0
39	291103.0
40	232.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.3282426698732	21.620022916880618	12.203926274599866	23.84780813864632
2	15.41407208329153	19.94049456105088	40.79858245321406	23.84685090244353
3	18.31156617356018	27.392389726823087	28.912658366344758	25.38338573327198
4	11.943390914392584	16.83419470307881	40.82460232871478	30.397812053813823
5	12.859260289730143	38.917845195143975	35.24056294356271	12.982331571563178
6	29.076074002901592	39.087858583424634	18.492217329468026	13.343850084205746
7	26.540158032189836	31.884175175210217	24.27945440712179	17.296212385478164
8	26.144829199765752	34.962612710571946	20.131358952379365	18.76119913728294
9	26.195129204271982	14.77298586180345	22.002260292590588	37.02962464133398
10-11	25.631656315053924	25.848612568632046	27.690119701736716	20.82961141457731
12-13	25.747207899760678	23.84056479296472	27.649846552960305	22.762380754314297
14-15	23.29225985172981	24.96202495477101	24.976864851899837	26.768850341599343
16-17	22.004844243378507	27.48848025723812	26.599107921101595	23.90756757828178
18-19	21.28350257446065	28.36610415057891	28.197726626739676	22.152666648220766
20-21	23.208546874053617	27.428669568452722	26.830229015191016	22.532554542302645
22-23	23.78888746182248	27.05733665624126	26.548410355862195	22.60536552607406
24-25	23.123949695448783	27.17176797463906	27.463868718558775	22.240413611353386
26-27	22.99085286791672	27.324388925425737	26.347128301066125	23.337629905591424
28-29	22.08464699268455	27.973391928551017	26.92098568487285	23.020975393891582
30-31	23.023511125208405	27.50118883031672	26.81157167743933	22.663728367035553
32-33	23.507159229373805	27.848189005908953	25.70172801769305	22.942923747024196
34-35	23.464687776089374	27.480579858994385	26.92822252296676	22.126509841949478
36-37	23.05188757519291	27.06085462279239	26.595988658055386	23.291269143959315
38-39	23.290052472128615	27.0685307190208	27.27741706200956	22.36399974684102
40-41	22.788935682410138	26.16646145585499	27.97102522623838	23.07357763549649
42-43	22.77282647961191	26.444564299737067	27.15888954518606	23.62371967546496
44-45	22.86422112630162	27.593268588456898	27.00944364396128	22.533066641280204
46-47	22.921043116545203	27.19520210103809	26.489770793191592	23.393983989225113
48-49	23.649518540513714	26.726024184796575	26.70595352197879	22.918503752710922
50-51	23.206251823091204	26.550889034607618	26.65515048231233	23.58770865998885
52-53	22.496262500212545	27.349654612661812	26.520517739428122	23.633565147697517
54-55	22.533320574520875	27.220845887129386	26.531014731095446	23.71481880725429
56-57	23.8188641871768	26.536422535803588	26.200447771840597	23.444265505179015
58-59	22.57240801778797	26.892957747329145	27.36892825941349	23.165705975469397
60-61	22.573581772739555	27.741193055657003	26.43809750323105	23.24712766837239
62-63	23.11736229969106	26.83301537824181	25.96461216671933	24.085010155347796
64-65	22.13354636734583	27.154833837997895	26.45341130270869	24.258208491947585
66-67	23.02336725939599	26.555918239795446	26.449450522141554	23.971263978667018
68-69	22.31122208336155	26.715884997603457	26.787907159940826	24.184985759094168
70-71	22.40541391692986	26.357871097503427	26.927404935993472	24.30931004957324
72-73	23.175736829686603	26.40611046797986	27.410532225906604	23.007620476426936
74-75	23.283078790542415	26.82666499238726	26.85797723704456	23.032278980025765
76-77	22.92649756251464	26.803193882503308	26.451142845415056	23.819165709567
78-79	22.69694998438119	26.761534169771643	26.629247269928474	23.91226857591869
80-81	22.282713079859302	27.132036111804492	27.355775485114915	23.229475323221298
82-83	23.301938113926486	25.91546864088344	28.430349367187436	22.352243878002646
84-85	23.63363900612338	26.640794357503538	26.785527418382387	22.94003921799069
86-87	22.064552385001036	27.360944832109173	27.398409333182343	23.17609344970744
88-89	23.294759267632475	25.977807633503737	27.09549145704803	23.631941641815754
90-91	22.927490644389877	25.942302677652872	27.412941953477095	23.717264724480152
92-93	23.31275574004153	26.26177904303263	27.205154840925367	23.220310376000473
94-95	21.916768289566928	26.279039433125856	27.996403202024506	23.80778907528271
96-97	22.403030564581076	26.161932792385624	27.410609910431067	24.024426732602237
98-99	21.695877575332858	26.998138362967516	27.301824290968636	24.004159770730986
100	22.657742044755043	26.15735372738137	26.37911350320549	24.80579072465809
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	17223.0
1	10096.0
2	2700.0
3	1918.0
4	1043.0
5	558.0
6	424.0
7	397.0
8	393.0
9	401.0
10	352.0
11	315.0
12	310.0
13	302.0
14	336.0
15	474.5
16	552.0
17	609.0
18	775.0
19	965.0
20	1150.0
21	1401.0
22	1896.5
23	2663.0
24	3738.0
25	5096.5
26	7593.5
27	10480.5
28	15379.5
29	26789.0
30	42769.0
31	57802.5
32	77909.0
33	95145.0
34	106670.5
35	121848.5
36	134753.0
37	159207.5
38	170122.5
39	155301.5
40	139431.5
41	124410.0
42	120346.5
43	128321.0
44	142776.5
45	174194.0
46	204203.0
47	224255.5
48	235706.0
49	229877.5
50	219264.5
51	225503.0
52	230477.5
53	221684.5
54	209836.0
55	180534.0
56	157120.0
57	129420.5
58	100968.0
59	96328.0
60	79539.0
61	54025.5
62	40275.5
63	33892.0
64	24869.5
65	12082.5
66	6807.5
67	4893.0
68	3678.0
69	2822.0
70	2189.0
71	1610.5
72	1231.0
73	1057.5
74	828.0
75	626.5
76	471.5
77	317.5
78	211.5
79	161.5
80	131.5
81	87.0
82	56.5
83	43.0
84	38.0
85	21.5
86	12.0
87	14.0
88	12.0
89	15.5
90	15.5
91	10.5
92	9.5
93	7.5
94	6.0
95	3.5
96	4.0
97	4.5
98	1.5
99	3.5
100	8.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9894703889466906
2	0.4472172371290187
3	0.4801835706362242
4	0.4664926343162919
5	0.43340620487645526
6	0.48548780766075944
7	0.5028016379483932
8	0.5043829010613678
9	0.5174733577181453
10-11	0.5318448376563198
12-13	0.5312043260156213
14-15	0.5263204247552946
16-17	0.5324753413026325
18-19	0.5307939982457988
20-21	0.5288824713180889
22-23	0.4935442431414213
24-25	0.5267507685138889
26-27	0.5079457470627537
28-29	0.5130998641715002
30-31	0.5161022624872749
32-33	0.5187743969883143
34-35	0.530173502593872
36-37	0.5478276046906269
38-39	0.5363584431243678
40-41	0.543073807357317
42-43	0.5414525122667987
44-45	0.5500193554611423
46-47	0.5487283241853592
48-49	0.5217267553321594
50-51	0.5357779794499846
52-53	0.529232751121596
54-55	0.5382599620576917
56-57	0.5261703048395059
58-59	0.5320349895496522
60-61	0.5256799131145959
62-63	0.5197051404662044
64-65	0.5310942380773762
66-67	0.5289124953012467
68-69	0.5483680363874663
70-71	0.5322451574317565
72-73	0.5369989547650663
74-75	0.5345069641629734
76-77	0.5230177866079424
78-79	0.5532018976758635
80-81	0.5281118557503733
82-83	0.5285522075033536
84-85	0.5375894264338353
86-87	0.8403312566077783
88-89	1.1050427081152425
90-91	1.095264897600203
92-93	1.0950547297180988
94-95	1.099768495073865
96-97	1.0932032507567044
98-99	1.0886195893279553
100	1.0843061437476256
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4996006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.79597044107855
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.06313139641037	17.386081150416192
2	12.508293143965785	5.952939478443105
3	5.136943514392576	3.6671566807792773
4	2.6633556286803297	2.535085272566292
5	1.587204185231632	1.8884531937864042
6	1.0003538438888544	1.428263429987909
7	0.6756563807650782	1.125452948550834
8	0.48736792186767197	0.9277914130154401
9	0.3734440077943772	0.7997816313785615
>10	1.8896301901487986	8.352356891868975
>50	0.2254409866659864	3.791718168379399
>100	0.278929405080206	14.813138537205663
>500	0.058070292080157744	9.624628473427224
>1k	0.0486443895974437	20.62821206283732
>5k	0.002861434682202571	4.5119044508036925
>10k+	6.732787487535462E-4	2.5670362165537104
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	20584	0.4120091128793681	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	17211	0.3444951827519823	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	17045	0.34117252861585834	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	15404	0.3083262910412838	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	15363	0.3075056355016387	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	14922	0.2986785844532613	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	14117	0.2825657134919374	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	13535	0.2709164080267318	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	9331	0.18676919122995447	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	9244	0.1850278002068052	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	8416	0.1684545615037292	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	8308	0.16629283471637143	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	8225	0.16463150764830947	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	8046	0.16104864565815175	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	7816	0.15644496824063062	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7707	0.15426322546450105	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	7390	0.14791815702383065	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	7308	0.1462768459445405	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	7240	0.14491575870805598	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	7094	0.14199342434736867	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	6959	0.1392912658631715	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	6684	0.133786868950918	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	6461	0.1293233034547997	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6422	0.1285426798926983	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	6418	0.12846261593761096	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	6408	0.12826245604989267	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	6256	0.12522002575657434	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	6223	0.12455949812710393	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5950	0.11909513319239408	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	5915	0.11839457358538	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5899	0.11807431776503072	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	5810	0.11629289476433775	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	5612	0.11232972898751523	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	5612	0.11232972898751523	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	5588	0.11184934525699129	No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG	5461	0.10930731468296875	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	5404	0.1081664033229744	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	5324	0.10656512422122792	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	5261	0.10530411692860257	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5187	0.10382293375948708	No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA	5161	0.10330251805141946	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5155	0.10318242211878849	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	4999	0.10005992787038287	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.001598877183094E-5
2	0.0	0.0	0.0	0.0	2.001598877183094E-5
3	0.0	0.0	0.0	0.0	2.001598877183094E-5
4	0.0	0.0	0.0	0.0	2.001598877183094E-5
5	0.0	6.0047966315492816E-5	0.0	0.0	2.001598877183094E-5
6	0.0	6.0047966315492816E-5	0.0	0.0	2.001598877183094E-5
7	0.0	6.0047966315492816E-5	0.0	0.0	2.001598877183094E-5
8	0.0	6.0047966315492816E-5	0.0	0.0	2.001598877183094E-5
9	0.0	6.0047966315492816E-5	0.0	0.0	2.001598877183094E-5
10-11	0.0	6.0047966315492816E-5	0.0	0.0	2.001598877183094E-5
12-13	0.0	7.005596070140828E-5	0.0	0.0	2.602078540338022E-4
14-15	0.0	8.006395508732376E-5	0.0	0.0	3.002398315774641E-4
16-17	0.0	8.006395508732376E-5	0.0	0.0	3.302638147352105E-4
18-19	0.0	8.006395508732376E-5	0.0	0.0	3.4027180912112596E-4
20-21	0.0	1.000799438591547E-4	0.0	0.0	3.803037866647878E-4
22-23	0.0	1.000799438591547E-4	0.0	2.001598877183094E-5	4.403517529802806E-4
24-25	0.0	1.000799438591547E-4	0.0	1.8014389894647844E-4	4.403517529802806E-4
26-27	0.0	1.000799438591547E-4	0.0	4.8038373052394247E-4	4.403517529802806E-4
28-29	0.0	1.000799438591547E-4	0.0	0.0018214549782366153	4.603677417521116E-4
30-31	0.0	1.2009593263098563E-4	0.0	0.007345867879261954	4.603677417521116E-4
32-33	0.0	1.6012791017464752E-4	0.0	0.0201861246763915	5.104077136816889E-4
34-35	0.0	1.7013590456056298E-4	0.0	0.04004198553804779	5.604476856112663E-4
36-37	0.0	2.001598877183094E-4	0.0	0.07633097318137728	5.604476856112663E-4
38-39	0.0	2.201758764901403E-4	0.0	0.13282610148987012	5.704556799971817E-4
40-41	0.0	2.201758764901403E-4	0.0	0.19442530693517981	5.804636743830972E-4
42-43	0.0	2.201758764901403E-4	0.0	0.2592470865727543	5.904716687690127E-4
44-45	0.0	2.4019186526197126E-4	0.0	0.33906084180043017	6.505196350845055E-4
46-47	0.0	2.501998596478867E-4	0.0	0.44031172100273697	7.205755957859139E-4
48-49	0.0	2.602078540338022E-4	0.0	0.5606778694821424	7.606075733295757E-4
50-51	0.0	2.602078540338022E-4	0.0	0.6931436831741196	7.806235621014066E-4
52-53	0.0	2.702158484197177E-4	0.0	0.8268304721811783	8.606875171887304E-4
54-55	0.0	3.903117810507033E-4	0.0	0.9748987491207977	8.706955115746458E-4
56-57	0.0	4.403517529802806E-4	0.0	1.1548424881795578	9.407514722760541E-4
58-59	0.0	4.603677417521116E-4	0.0	1.3756188443328532	9.407514722760541E-4
60-61	0.0	4.7037573613802704E-4	0.0	1.6064632428383794	9.407514722760541E-4
62-63	0.0	4.803837305239425E-4	0.0	1.852159505012604	0.0010608474049070397
64-65	0.0	4.803837305239425E-4	0.0	2.1072432659208173	0.0010808633936788706
66-67	0.0	4.803837305239425E-4	0.0	2.404470691188121	0.0011008793824507017
68-69	0.0	5.104077136816889E-4	0.0	2.740148830886112	0.001130903365608448
70-71	0.0	5.804636743830972E-4	0.0	3.1126063499523418	0.0011709353431521097
72-73	0.0	6.405116406985901E-4	0.0	3.4984145335293833	0.0012309833094676027
74-75	0.0	6.405116406985901E-4	0.0	3.8783079924243484	0.0013110472645549264
76-77	0.0	6.405116406985901E-4	0.0	4.319180161112697	0.0013811032252563347
78-79	0.0	6.405116406985901E-4	0.0	4.794419782522279	0.0014311431971859123
80-81	0.0	6.905516126281673E-4	0.0	5.315916353983562	0.001471175174729574
82-83	0.0	7.005596070140829E-4	0.0	5.8596506889703495	0.0015412311354309823
84-85	0.0	7.005596070140829E-4	0.0	6.382007947948821	0.0015812631129746442
86-87	0.0	7.405915845577447E-4	0.0	6.943396385032363	0.0016112870961323906
88	0.0	7.405915845577447E-4	0.0	7.390563582189453	0.0016413110792901369
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5185	0.0	38.61984	94
GTACTAG	5860	0.0	35.428867	1
GTATCAA	10935	0.0	33.1394	1
TATTCTC	2585	0.0	32.716305	5
CAACGCA	9655	0.0	32.701622	5
GCATCGT	6105	0.0	31.452042	8
TAGGCAT	7370	0.0	31.237831	5
GGTATCA	6900	0.0	30.7727	1
ATCAACG	10435	0.0	30.397812	3
AGGGCTA	6290	0.0	28.683548	5
CTAGGCA	8305	0.0	28.628183	4
CTGTGCG	7670	0.0	28.09553	9
TAGTACT	7690	0.0	27.67928	4
TATCAAC	13040	0.0	27.641241	2
TACCCCG	315	0.0	26.848139	5
GCTATTG	6775	0.0	26.401394	8
GTACACG	305	0.0	26.299728	1
AACGCAG	12685	0.0	26.238077	6
ACGCAGA	11965	0.0	26.20969	7
ACTGTGC	8305	0.0	26.172945	8
>>END_MODULE