##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146483_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4996006
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.818681963152166	31.0	31.0	34.0	30.0	34.0
2	31.82020978357512	31.0	31.0	34.0	30.0	34.0
3	31.994598485270032	33.0	31.0	34.0	30.0	34.0
4	35.7288043689299	37.0	35.0	37.0	35.0	37.0
5	35.637256440444624	37.0	35.0	37.0	33.0	37.0
6	35.665554044570804	37.0	35.0	37.0	33.0	37.0
7	35.58086039128055	37.0	35.0	37.0	33.0	37.0
8	35.5667657324671	37.0	35.0	37.0	33.0	37.0
9	37.16014812632331	39.0	37.0	39.0	33.0	39.0
10-11	37.1031847239575	39.0	37.0	39.0	33.0	39.0
12-13	37.03630219819592	39.0	37.0	39.0	33.0	39.0
14-15	38.215329305048876	40.0	37.0	41.0	33.0	41.0
16-17	38.22462983431164	40.0	37.0	41.0	33.0	41.0
18-19	38.277244763116784	40.0	37.5	41.0	33.0	41.0
20-21	38.2961362536394	40.0	38.0	41.0	33.0	41.0
22-23	38.25909566561769	40.0	37.5	41.0	33.0	41.0
24-25	38.18409425449049	40.0	37.0	41.0	33.0	41.0
26-27	38.03537135463809	40.0	37.0	41.0	33.0	41.0
28-29	37.97169789227635	40.0	37.0	41.0	33.0	41.0
30-31	37.844335955561306	40.0	37.0	41.0	33.0	41.0
32-33	37.71986332682547	40.0	36.0	41.0	32.0	41.0
34-35	37.621870550195496	40.0	36.0	41.0	32.0	41.0
36-37	37.541143165160335	40.0	36.0	41.0	32.0	41.0
38-39	37.4181608268685	40.0	36.0	41.0	31.5	41.0
40-41	37.286472234020536	39.5	36.0	41.0	31.0	41.0
42-43	37.15937470851716	39.0	35.0	41.0	31.0	41.0
44-45	36.94878208713121	39.0	35.0	41.0	31.0	41.0
46-47	36.709992542042585	39.0	35.0	41.0	30.0	41.0
48-49	36.43982653343491	39.0	35.0	41.0	30.0	41.0
50-51	36.231544557792766	39.0	35.0	41.0	29.0	41.0
52-53	36.02845733171658	38.0	35.0	40.0	29.0	41.0
54-55	35.81884579402026	38.0	34.5	40.0	29.0	41.0
56-57	35.54249524520186	37.5	34.0	40.0	28.0	41.0
58-59	35.251292632554886	37.0	34.0	40.0	28.0	41.0
60-61	34.98802893751528	36.5	34.0	40.0	28.0	41.0
62-63	34.71280288694609	36.0	34.0	39.0	27.0	41.0
64-65	34.4359535396875	35.0	33.0	39.0	27.0	41.0
66-67	34.22004757400211	35.0	33.0	39.0	26.5	41.0
68-69	34.0346051425879	35.0	33.0	38.0	26.0	41.0
70-71	33.84792612338736	35.0	33.0	37.5	26.0	40.0
72-73	33.66567484106304	35.0	33.0	37.0	26.0	40.0
74-75	33.49455995449165	35.0	33.0	36.5	26.0	39.5
76-77	33.34858334837868	35.0	33.0	36.0	27.0	39.0
78-79	33.24229084192453	35.0	33.0	36.0	27.0	39.0
80-81	33.11112866958126	35.0	33.0	36.0	27.0	38.0
82-83	32.92251670634503	35.0	33.0	35.0	27.0	37.0
84-85	32.78121163185152	35.0	33.0	35.0	26.5	37.0
86-87	32.65416975079694	35.0	33.0	35.0	26.0	36.5
88-89	32.54875914880807	35.0	33.0	35.0	26.0	36.0
90-91	32.460223426473064	35.0	33.0	35.0	26.0	36.0
92-93	32.377395063176465	35.0	33.0	35.0	26.0	36.0
94-95	32.339592866781985	35.0	33.0	35.0	26.0	35.0
96-97	32.28786404579978	35.0	33.0	35.0	26.0	35.0
98-99	32.24868544993741	35.0	33.0	35.0	26.0	35.0
100	31.740822969387946	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5186410261424257
1101	2	0.6148810020906055
1101	3	0.5203100250823525
1101	4	0.3797840771610197
1101	5	0.37534909335867894
1101	6	0.4281231172550761
1101	7	0.40869357626091585
1101	8	0.4484333889264889
1101	9	0.5334525945486632
1101	10-11	0.5695470166934626
1101	12-13	0.5533014830588385
1101	14-15	0.7399744557606027
1101	16-17	0.690889946147152
1101	18-19	0.6763766864934553
1101	20-21	0.7110781307768761
1101	22-23	0.7232012860444073
1101	24-25	0.7063885432031611
1101	26-27	0.6995237452378831
1101	28-29	0.7519012113945891
1101	30-31	0.8129181175556539
1101	32-33	0.8250392944417442
1101	34-35	0.7413477663893104
1101	36-37	0.7647277252021354
1101	38-39	0.770141297241608
1101	40-41	0.78753239047996
1101	42-43	0.7874740502211637
1101	44-45	0.7736923158385309
1101	46-47	0.7445820000373544
1101	48-49	0.8082937386087394
1101	50-51	0.7989354870699046
1101	52-53	0.7431631277973736
1101	54-55	0.679893773900119
1101	56-57	0.69462531891444
1101	58-59	0.7165817090042523
1101	60-61	0.7725535838107049
1101	62-63	0.7282757984375436
1101	64-65	0.7219830304768067
1101	66-67	0.6167524150126837
1101	68-69	0.6439730863738404
1101	70-71	0.600855300673615
1101	72-73	0.5988503416066138
1101	74-75	0.59812070121842
1101	76-77	0.6125092888511929
1101	78-79	0.5705303797717889
1101	80-81	0.489974992181736
1101	82-83	0.5468459677594595
1101	84-85	0.5824721782349371
1101	86-87	0.5823874812107093
1101	88-89	0.5406707526443029
1101	90-91	0.5513790282318212
1101	92-93	0.569411385104317
1101	94-95	0.5361565140895763
1101	96-97	0.5439615494879106
1101	98-99	0.5666870612570847
1101	100	0.579979962451997
1102	1	0.4077702835331536
1102	2	0.47667264946948507
1102	3	0.37515848346260583
1102	4	0.2612336549588292
1102	5	0.271776600513121
1102	6	0.2746248452375326
1102	7	0.3236370941769451
1102	8	0.28880831564043064
1102	9	0.3622718149406978
1102	10-11	0.382745030459688
1102	12-13	0.3929128354036209
1102	14-15	0.48187176145915345
1102	16-17	0.49262753581607654
1102	18-19	0.4764517273579827
1102	20-21	0.4511327429518843
1102	22-23	0.47405073854965707
1102	24-25	0.5023049456713622
1102	26-27	0.524918222299469
1102	28-29	0.5444310887479773
1102	30-31	0.5787324653508961
1102	32-33	0.5045323290590957
1102	34-35	0.5356716805123796
1102	36-37	0.5418862061363967
1102	38-39	0.5205245138476329
1102	40-41	0.5398402102202837
1102	42-43	0.5870091647490412
1102	44-45	0.5956301885178519
1102	46-47	0.5918124884533569
1102	48-49	0.6116160864620639
1102	50-51	0.5552016193135074
1102	52-53	0.48637570812051223
1102	54-55	0.4884061817673242
1102	56-57	0.523248953074031
1102	58-59	0.4788683010837076
1102	60-61	0.45433094675533425
1102	62-63	0.3957879302759366
1102	64-65	0.4592085542864197
1102	66-67	0.5103241073822318
1102	68-69	0.4460130739122903
1102	70-71	0.40020850251372053
1102	72-73	0.4126267632396079
1102	74-75	0.3520448016477644
1102	76-77	0.32820165057216144
1102	78-79	0.35421990612245935
1102	80-81	0.3150545639361013
1102	82-83	0.3651330228209986
1102	84-85	0.3613083109397124
1102	86-87	0.36047346858470775
1102	88-89	0.35524018344341357
1102	90-91	0.4099320579996757
1102	92-93	0.371001424624815
1102	94-95	0.35017956734733957
1102	96-97	0.33014731589592117
1102	98-99	0.3659354639350454
1102	100	0.37089276917292935
1103	1	0.28243893902524775
1103	2	-0.1469478760890368
1103	3	0.20039421333131457
1103	4	0.22234131040534777
1103	5	0.23909604605859158
1103	6	0.24849419143858142
1103	7	0.31697113362809404
1103	8	0.3234821909952075
1103	9	0.34072312385909953
1103	10-11	0.3885233548574547
1103	12-13	0.3938399713908396
1103	14-15	0.5285927030753115
1103	16-17	0.5422494630842607
1103	18-19	0.5311475607109628
1103	20-21	0.5031742019360337
1103	22-23	0.45512314438992263
1103	24-25	0.4863088917442795
1103	26-27	0.5312979986318993
1103	28-29	0.5792139153047131
1103	30-31	0.5707156067513921
1103	32-33	0.6045800961752903
1103	34-35	0.5459469191716195
1103	36-37	0.5065657596725686
1103	38-39	0.5753033946238446
1103	40-41	0.5716883990554891
1103	42-43	0.5462250402804969
1103	44-45	0.5886271590457994
1103	46-47	0.528886636270208
1103	48-49	0.5687624739329564
1103	50-51	0.4899934112274522
1103	52-53	0.5105177283696491
1103	54-55	0.5190402646052519
1103	56-57	0.5049956155434643
1103	58-59	0.4313928513785825
1103	60-61	0.48126299128475836
1103	62-63	0.5355371718297448
1103	64-65	0.4711088964282766
1103	66-67	0.5285796743738231
1103	68-69	0.4926522198927614
1103	70-71	0.45216497700194225
1103	72-73	0.4298070264570839
1103	74-75	0.4347581163873997
1103	76-77	0.468073887104957
1103	78-79	0.406593691856564
1103	80-81	0.4112702347591721
1103	82-83	0.4151844411364891
1103	84-85	0.4104098783494976
1103	86-87	0.41575150192815613
1103	88-89	0.39640626758115616
1103	90-91	0.45794033061582695
1103	92-93	0.41008813432154056
1103	94-95	0.4433769773861158
1103	96-97	0.38598487999703934
1103	98-99	0.46131534271303565
1103	100	0.48724651271027497
1104	1	0.35554606059891114
1104	2	0.4112270546156296
1104	3	0.34563841896906666
1104	4	0.2643948640109599
1104	5	0.26981294650124
1104	6	0.28067507263638447
1104	7	0.28364271382393724
1104	8	0.31910740387336745
1104	9	0.35675034714662957
1104	10-11	0.32613717463873115
1104	12-13	0.3792581860389177
1104	14-15	0.44635367652164604
1104	16-17	0.48315713115245984
1104	18-19	0.4995130682357356
1104	20-21	0.43983339608347904
1104	22-23	0.45794255495098213
1104	24-25	0.48536290439308516
1104	26-27	0.5488403211864821
1104	28-29	0.5155312554879927
1104	30-31	0.5533587644301647
1104	32-33	0.4988912458668793
1104	34-35	0.4798368238778252
1104	36-37	0.508064085406879
1104	38-39	0.4897514999501169
1104	40-41	0.4706364851971401
1104	42-43	0.403117968316252
1104	44-45	0.4318159200225793
1104	46-47	0.39842694676408286
1104	48-49	0.44249635841879353
1104	50-51	0.39720419684145014
1104	52-53	0.4031577519823415
1104	54-55	0.37564756557072343
1104	56-57	0.3594688704143678
1104	58-59	0.39000171354884827
1104	60-61	0.37270443583597057
1104	62-63	0.3862086283142716
1104	64-65	0.30945963804045107
1104	66-67	0.32936250746270446
1104	68-69	0.2681425950766183
1104	70-71	0.35706214258367197
1104	72-73	0.39158109068302593
1104	74-75	0.3009782561166503
1104	76-77	0.3070896203664475
1104	78-79	0.285594205435423
1104	80-81	0.31790997438399415
1104	82-83	0.282534877598728
1104	84-85	0.2943668581061445
1104	86-87	0.27801728564901396
1104	88-89	0.27542478625514377
1104	90-91	0.27475115888697843
1104	92-93	0.2636023663605229
1104	94-95	0.24469307698711162
1104	96-97	0.2660397497438751
1104	98-99	0.21177440325503483
1104	100	0.3617727167845324
1105	1	0.1257575789921681
1105	2	-0.3318538565269762
1105	3	0.0404965709204248
1105	4	0.12991223167706778
1105	5	0.16477796612922901
1105	6	0.2079359822008442
1105	7	0.23304823725539592
1105	8	0.21933141328468508
1105	9	0.2859552902126268
1105	10-11	0.29762081012605535
1105	12-13	0.3279218805440607
1105	14-15	0.3087934308780049
1105	16-17	0.38421324316512795
1105	18-19	0.4038759941644585
1105	20-21	0.3928631022293487
1105	22-23	0.3181549441929761
1105	24-25	0.40656737030946033
1105	26-27	0.38691363546691093
1105	28-29	0.3990063923723355
1105	30-31	0.403022526660223
1105	32-33	0.3833569644453334
1105	34-35	0.4286080290618841
1105	36-37	0.4277373235334778
1105	38-39	0.42703483177262314
1105	40-41	0.39990917064930187
1105	42-43	0.39489846851750343
1105	44-45	0.4252534440696394
1105	46-47	0.4494406765400072
1105	48-49	0.37983341937142256
1105	50-51	0.37856068666688003
1105	52-53	0.35712637249295653
1105	54-55	0.33571640804991176
1105	56-57	0.4355027796284574
1105	58-59	0.3652645162657606
1105	60-61	0.36470404605078954
1105	62-63	0.3548320656352857
1105	64-65	0.3772768431178477
1105	66-67	0.4281119134718807
1105	68-69	0.3788007701061744
1105	70-71	0.3187444926814891
1105	72-73	0.3651097617000687
1105	74-75	0.3145530562565142
1105	76-77	0.22523223351790733
1105	78-79	0.22109278660197873
1105	80-81	0.2369950314235325
1105	82-83	0.2325079815824722
1105	84-85	0.2833654299421866
1105	86-87	0.29682975864903227
1105	88-89	0.2592670040510612
1105	90-91	0.2463432922713693
1105	92-93	0.1621083453381189
1105	94-95	0.23818400691587271
1105	96-97	0.23228240125880006
1105	98-99	0.20877801266442475
1105	100	0.17327939621526411
1106	1	0.2422327897873302
1106	2	0.27351225842230775
1106	3	0.2162927922694422
1106	4	0.13506074456990547
1106	5	0.15902078818481868
1106	6	0.18824050359054922
1106	7	0.20229176575880103
1106	8	0.19870492872512813
1106	9	0.2474942458958509
1106	10-11	0.24919270536133808
1106	12-13	0.25183343617943876
1106	14-15	0.3364801469191505
1106	16-17	0.3364507762066822
1106	18-19	0.36239176529629447
1106	20-21	0.3731384249702856
1106	22-23	0.3343585188565825
1106	24-25	0.31418350858450594
1106	26-27	0.30591856379385973
1106	28-29	0.3106003201786933
1106	30-31	0.2996198259742613
1106	32-33	0.3101513558033062
1106	34-35	0.3293066410034484
1106	36-37	0.32757484859758534
1106	38-39	0.34011498782439276
1106	40-41	0.29317277090774496
1106	42-43	0.2790349689661866
1106	44-45	0.37127392268844517
1106	46-47	0.36852044264333017
1106	48-49	0.2722457432833636
1106	50-51	0.2663952645220604
1106	52-53	0.3337059741466959
1106	54-55	0.337491388443631
1106	56-57	0.37873127968858
1106	58-59	0.3240180785412221
1106	60-61	0.25435788160016415
1106	62-63	0.24675060178150687
1106	64-65	0.2786398923075666
1106	66-67	0.27536521656118396
1106	68-69	0.25296504949884735
1106	70-71	0.2747742394298456
1106	72-73	0.30298136243844453
1106	74-75	0.21403957933750917
1106	76-77	0.23331184636192148
1106	78-79	0.2577879891018213
1106	80-81	0.24184396922614582
1106	82-83	0.2523107219979579
1106	84-85	0.20909090347591075
1106	86-87	0.2215190071689932
1106	88-89	0.2088743292618389
1106	90-91	0.19615584124674967
1106	92-93	0.22360171206631918
1106	94-95	0.22167497980284168
1106	96-97	0.216519933153549
1106	98-99	0.23859235065060602
1106	100	0.30069805271294925
1107	1	0.07756819751631738
1107	2	-0.48739319818720617
1107	3	-0.05003564254565518
1107	4	0.024654960543593063
1107	5	0.06223634566382685
1107	6	0.14937129692303586
1107	7	0.14300518203307178
1107	8	0.13021702570329552
1107	9	0.1479970932184429
1107	10-11	0.1672848910717306
1107	12-13	0.09105904223195438
1107	14-15	0.15268419125804655
1107	16-17	0.2507206890618292
1107	18-19	0.21409396636754252
1107	20-21	0.17668119865287935
1107	22-23	0.1244006957928363
1107	24-25	0.07114267690047171
1107	26-27	0.1749010371391293
1107	28-29	0.1384217302723414
1107	30-31	0.15947223722371717
1107	32-33	0.22138505779017237
1107	34-35	0.21545248818265605
1107	36-37	0.20354301328771385
1107	38-39	0.20326889256197944
1107	40-41	0.27676672941355207
1107	42-43	0.24613928741416657
1107	44-45	0.19635361388049688
1107	46-47	0.22903948811431007
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2216	80-81	-0.5914628954022589
2216	82-83	-0.5654297341001282
2216	84-85	-0.6874121243505584
2216	86-87	-0.6413845302948644
2216	88-89	-0.5978640893502742
2216	90-91	-0.6194218255477892
2216	92-93	-0.6187798997013516
2216	94-95	-0.5383311877679944
2216	96-97	-0.611911877963351
2216	98-99	-0.6170406475212395
2216	100	-0.6637266783879703
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	7.0
12	10.0
13	55.0
14	313.0
15	947.0
16	2340.0
17	4606.0
18	8078.0
19	12291.0
20	18051.0
21	25682.0
22	34771.0
23	40702.0
24	42968.0
25	45238.0
26	50404.0
27	61793.0
28	78101.0
29	98212.0
30	120416.0
31	149753.0
32	185283.0
33	234786.0
34	323690.0
35	442204.0
36	661138.0
37	1105807.0
38	886754.0
39	360874.0
40	732.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.046743658577114	22.061485427389183	13.40181729735573	24.48995361667798
2	14.965530632098547	20.327959421427057	40.11055419217173	24.595955754302665
3	17.869305682937735	27.10039148085771	29.800944854402665	25.229357981801886
4	12.158410536836643	17.406947354258364	39.43333109090793	31.001311017997065
5	12.435779723777001	38.942262751529206	35.31684338189124	13.305114142802555
6	28.944921203057	39.43628170182342	17.992732594796724	13.626064500322856
7	25.588141689635087	32.56693868482523	24.36864365220907	17.47627597333061
8	26.304992427951447	34.746515516594656	19.799295677387097	19.149196378066797
9	26.16926400808966	14.24976271045311	21.983200180304028	37.5977731011532
10-11	25.606274291904374	25.96091758096367	27.39654435963448	21.036263767497477
12-13	25.891582195858053	23.864032589232277	27.941089342166524	22.30329587274315
14-15	23.281211121780572	24.759681934002685	25.66272256983041	26.296384374386335
16-17	21.536862673901737	27.1672224943368	27.26966746219314	24.02624736956832
18-19	20.903307562080588	27.340679734972294	29.813825283636568	21.942187419310546
20-21	22.55745694257128	26.453702380423387	28.899888350725238	22.088952326280094
22-23	23.072661173897924	26.094701471438185	28.586153318804936	22.246484035858956
24-25	22.481384874653333	26.18640555668034	29.53764353094558	21.79456603772074
26-27	22.401625942542573	26.355193145228643	28.417875560111206	22.82530535211758
28-29	21.514185531402486	27.073266124980634	28.952947614554507	22.459600729062377
30-31	22.56243287137766	26.519293611737055	28.731180466957007	22.187093049928283
32-33	23.074551952099338	26.872305597711453	27.79121962623744	22.26192282395177
34-35	22.93839228730897	26.589262332956732	28.784950520654284	21.687394859080015
36-37	22.632488832079066	26.115150782445017	28.493350488370112	22.75900989710581
38-39	22.81017076440661	26.039600432825743	29.266027702929097	21.884201099838553
40-41	22.267550843327193	25.355090137380316	29.73933068253808	22.638028336754417
42-43	22.454255884817577	25.607016694754304	28.717763645722087	23.220963774706036
44-45	22.580567357204938	26.691451131163575	28.4253561745122	22.30262533711929
46-47	22.733689671309442	26.394363817817673	27.69955640565684	23.172390105216046
48-49	23.674779257478864	26.117113382582275	27.603648854408714	22.604458505530147
50-51	23.026896187364084	26.115033300103452	27.305954727231587	23.552115785300877
52-53	22.179587054138846	26.871565006126897	27.22251534525779	23.726332594476467
54-55	22.39247996517205	26.797625094138045	27.005832180324713	23.804062760365195
56-57	23.640119731773446	26.05348017879502	26.77919067462063	23.52720941481091
58-59	22.270747777268703	26.477338939426055	27.938660042157785	23.313253241147454
60-61	22.4283226581265	27.254523618895117	27.006204963971175	23.310948759007204
62-63	22.995318660546044	26.32654964785871	26.519373675692144	24.158758015903103
64-65	21.983300260247887	26.601088949853143	26.997865895277144	24.417744894621823
66-67	22.72011883092214	26.118903780339735	27.00873457718025	24.152242811557873
68-69	22.19274604471054	26.008336975459954	27.444704311193707	24.3542126686358
70-71	22.188561701286126	25.695494250706297	27.56955793194163	24.546386116065946
72-73	22.87634362328628	25.677721363825423	28.0080428246083	23.43789218828
74-75	22.941500730385183	26.24719925604394	27.414494492987785	23.39680552058309
76-77	22.658061419375397	26.215942719846495	26.85694263447592	24.269053226302184
78-79	22.26623537336958	26.305221506524795	27.15128148492944	24.277261635176192
80-81	21.879940350490905	26.763443658236337	27.791637058758774	23.564978932513984
82-83	22.888993196434683	25.473167050647426	28.912020032386494	22.725819720531394
84-85	23.29440813260624	26.148316774901577	27.128150902002073	23.429124190490107
86-87	21.623979366690143	26.8958364756747	27.704586315409035	23.77559784222612
88-89	22.64080409783708	25.63091882054067	27.480001945707944	24.248275135914305
90-91	22.23932687920279	25.509829372789543	27.83570241339804	24.41514133460963
92-93	22.681926186601327	25.921346312833172	27.311507996084533	24.08521950448097
94-95	21.21283421732027	25.820868843934036	28.214719353329095	24.751577585416598
96-97	21.69454782382852	25.70966465045591	27.626721273000143	24.96906625271543
98-99	20.982880290942077	26.538506459836086	27.564936387445847	24.91367686177599
100	21.905468208641366	25.711604735864814	26.65381353869491	25.72911351679891
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	162.0
1	118.0
2	115.0
3	154.0
4	106.0
5	80.0
6	190.5
7	437.5
8	717.5
9	852.0
10	688.5
11	463.0
12	473.0
13	576.0
14	710.5
15	1101.0
16	1585.0
17	2067.0
18	2744.0
19	3693.0
20	4755.5
21	5823.5
22	7004.0
23	8117.0
24	9182.5
25	10458.5
26	12561.5
27	14909.0
28	19083.0
29	29726.0
30	44973.5
31	58739.5
32	78623.0
33	95868.5
34	104999.0
35	118433.5
36	131497.0
37	156912.5
38	167949.0
39	152200.0
40	136969.5
41	122070.5
42	117105.0
43	124890.5
44	138329.5
45	171168.0
46	203520.0
47	223965.5
48	236544.5
49	229200.5
50	217182.0
51	222138.5
52	226913.5
53	220479.5
54	213722.0
55	184374.0
56	158670.5
57	131396.5
58	102046.0
59	99149.5
60	81789.0
61	54347.5
62	39490.5
63	32725.5
64	23936.5
65	11126.0
66	6041.5
67	4375.0
68	3317.0
69	2621.5
70	2015.0
71	1454.0
72	1104.0
73	941.0
74	677.0
75	496.5
76	376.5
77	228.5
78	124.0
79	70.5
80	62.0
81	43.5
82	25.5
83	8.5
84	1.0
85	1.0
86	1.5
87	1.0
88	0.0
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013530808409757716
2	0.00532425301330703
3	0.0020816628322704177
4	0.028302608123368943
5	0.002301838708760558
6	0.0
7	3.20255820349295E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008787019070833782
16-17	0.0030624462820901337
18-19	0.0
20-21	8.006395508732375E-5
22-23	8.706955115746458E-4
24-25	3.0023983157746408E-5
26-27	2.301838708760558E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	6.505196350845055E-4
36-37	0.0
38-39	0.0
40-41	4.203357642084497E-4
42-43	7.105676013999983E-4
44-45	0.0
46-47	0.0
48-49	5.404316968394354E-4
50-51	1.0007994385915468E-5
52-53	0.0
54-55	3.7029579227887237E-4
56-57	1.1008793824507017E-4
58-59	2.602078540338022E-4
60-61	1.2009593263098563E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0013911112196422503
70-71	8.907115003464767E-4
72-73	0.0
74-75	2.4019186526197126E-4
76-77	0.0027922304336704157
78-79	4.90391724909858E-4
80-81	0.0031224942484056263
82-83	0.00192153492209577
84-85	0.006275012479969
86-87	0.007896307570487305
88-89	0.007906315564873222
90-91	0.005814644738216888
92-93	0.00872697110451829
94-95	0.0016012791017464751
96-97	0.004973973209799989
98-99	0.0019515589052535166
100	0.0845675525609857
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4996006.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.73639323538936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.57423178241132	11.630503610014141
2	15.640578515790201	5.54814901970075
3	6.589540940436714	3.5062406808084896
4	3.4201410158937193	2.4264386391350024
5	2.081247302598143	1.8456910289487032
6	1.2854122958986818	1.3679146769798212
7	0.8935041737534101	1.10932789682065
8	0.6375461205566474	0.904621495991172
9	0.47082018447045193	0.7515586741433845
>10	2.531403475392311	8.367891391778366
>50	0.31189045634835233	3.9276168260342597
>100	0.39789872727744163	15.740120399387001
>500	0.08639341418056962	10.700213905248056
>1k	0.07351910147915035	23.616144552167384
>5k	0.0050819655400334346	6.105214100454841
>10k+	7.905279728940898E-4	2.452353102388075
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	22647	0.4533020977156552	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	18036	0.36100837348874276	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	17109	0.3424535518972555	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	17096	0.34219334404322177	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	16996	0.3401917451660386	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	15865	0.31755366186509787	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	14684	0.2939147791255655	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	9886	0.19787806499832064	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	9415	0.1884505342867883	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	9266	0.18546815195978547	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9167	0.1834865690713742	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8566	0.17145695981950382	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	8548	0.17109667202161088	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	8307	0.1662728187275996	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	8153	0.16319035645673766	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	7951	0.15914712672482778	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	7802	0.156164744397825	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	7676	0.15364272981257426	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	7629	0.15270197834029825	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	7611	0.15234169054240526	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	7558	0.15128084313749823	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	7418	0.1484786047094419	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	7298	0.1460766860568222	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7233	0.1447756467866532	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	7210	0.14431527904490105	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	7088	0.1418733284147377	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	7072	0.1415530725943884	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	6938	0.13887093009896304	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	6619	0.13248582968074898	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	6571	0.1315250622197011	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	6477	0.12964355927514898	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	6432	0.1287428397804166	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	6110	0.12229769139588703	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	6106	0.12221762744079971	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	5932	0.11873484539450113	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	5877	0.11763396601205042	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	5796	0.11601267092153213	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	5780	0.11569241510118282	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	5686	0.11381091215663071	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	5645	0.11299025661698565	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	5641	0.11291019266189833	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	5535	0.11078849785208425	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	5512	0.11032813011033213	No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC	5457	0.10922725072788142	No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT	5451	0.10910715479525045	No Hit
GTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTTTTATGGATAATTG	5330	0.10668522015385891	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	5272	0.10552429280509269	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5257	0.10522405297351525	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	5222	0.10452349336650116	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	5162	0.1033225340401913	No Hit
CTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA	5076	0.10160115900581385	No Hit
GGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGA	5063	0.10134095115178005	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.006395508732376E-5
2	0.0	0.0	0.0	0.0	8.006395508732376E-5
3	0.0	0.0	0.0	0.0	8.006395508732376E-5
4	0.0	0.0	0.0	0.0	8.006395508732376E-5
5	0.0	1.000799438591547E-4	0.0	0.0	1.000799438591547E-4
6	0.0	1.000799438591547E-4	0.0	0.0	1.4011192140281657E-4
7	0.0	1.2009593263098563E-4	0.0	0.0	1.4011192140281657E-4
8	0.0	1.2009593263098563E-4	0.0	0.0	1.4011192140281657E-4
9	0.0	1.2009593263098563E-4	0.0	0.0	1.4011192140281657E-4
10-11	0.0	1.2009593263098563E-4	0.0	0.0	1.4011192140281657E-4
12-13	0.0	1.7013590456056298E-4	0.0	0.0	2.8022384280563313E-4
14-15	0.0	1.8014389894647844E-4	0.0	0.0	3.7029579227887237E-4
16-17	0.0	1.8014389894647844E-4	0.0	0.0	4.803837305239425E-4
18-19	0.0	2.001598877183094E-4	0.0	0.0	5.204157080676044E-4
20-21	0.0	2.201758764901403E-4	0.0	0.0	5.604476856112663E-4
22-23	0.0	2.201758764901403E-4	0.0	2.001598877183094E-5	6.405116406985901E-4
24-25	0.0	2.201758764901403E-4	0.0	1.901518933323939E-4	6.405116406985901E-4
26-27	0.0	2.201758764901403E-4	0.0	5.003997192957734E-4	6.60527629470421E-4
28-29	0.0	2.201758764901403E-4	0.0	0.0018414709670084464	6.805436182422519E-4
30-31	0.0	2.201758764901403E-4	0.0	0.0075260117782084334	6.905516126281673E-4
32-33	0.0	2.201758764901403E-4	0.0	0.02056642846305629	7.005596070140829E-4
34-35	0.0	2.702158484197177E-4	0.0	0.040632457206816804	7.005596070140829E-4
36-37	0.0	3.302638147352105E-4	0.0	0.07760198846838855	7.005596070140829E-4
38-39	0.0	3.6028779789295693E-4	0.0	0.13487774033898278	7.205755957859138E-4
40-41	0.0	3.8030378666478786E-4	0.0	0.19740768926218263	7.205755957859138E-4
42-43	0.0	3.9031178105070335E-4	0.0	0.26354051616431207	7.205755957859138E-4
44-45	0.0	4.003197754366188E-4	0.0	0.3447553906060161	7.405915845577447E-4
46-47	0.0	4.003197754366188E-4	0.0	0.44804790066304967	7.706155677154911E-4
48-49	0.0	4.003197754366188E-4	0.0	0.5705757759298127	7.906315564873221E-4
50-51	0.0	4.003197754366188E-4	0.0	0.705273372369849	8.206555396450684E-4
52-53	0.0	4.103277698225342E-4	0.0	0.8409417442653191	9.207354835042232E-4
54-55	0.0	5.003997192957734E-4	0.0	0.9914219478519442	9.60767461047885E-4
56-57	0.0	5.604476856112663E-4	0.0	1.173527413698062	9.907914442056315E-4
58-59	0.0	6.004796631549281E-4	0.0	1.396805768447836	0.0010408314161352088
60-61	0.0	6.004796631549281E-4	0.0	1.6298118937407202	0.0010408314161352088
62-63	0.0	6.004796631549281E-4	0.0	1.8774497068258125	0.0010808633936788706
64-65	0.0	6.605276294704209E-4	0.0	2.1356859859655892	0.0011208953712225325
66-67	0.0	7.005596070140829E-4	0.0	2.43583574559358	0.0011409113599943634
68-69	0.0	7.405915845577447E-4	0.0	2.774466243635416	0.0011409113599943634
70-71	0.0	7.806235621014066E-4	0.0	3.1508468965009246	0.0011509193543802788
72-73	2.001598877183094E-5	8.206555396450684E-4	0.0	3.5413788534281183	0.0012009593263098564
74-75	2.001598877183094E-5	8.206555396450684E-4	0.0	3.9267667012409513	0.0012509992982394336
76-77	2.001598877183094E-5	8.206555396450684E-4	0.0	4.375785377359435	0.001261007292625349
78-79	2.001598877183094E-5	8.406715284168994E-4	0.0	4.86018231363213	0.001261007292625349
80-81	2.001598877183094E-5	8.606875171887304E-4	0.0	5.392397447080729	0.001261007292625349
82-83	2.001598877183094E-5	8.606875171887304E-4	0.0	5.948481647139735	0.001301039270169011
84-85	2.001598877183094E-5	8.606875171887304E-4	0.0	6.4829786033083225	0.0013110472645549264
86-87	2.001598877183094E-5	9.307434778901386E-4	0.0	7.05562603407602	0.0013811032252563347
88	2.001598877183094E-5	9.407514722760541E-4	0.0	7.512060634034467	0.0014411511915718275
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9900	0.0	44.079895	1
GTGGTAT	2125	0.0	40.496727	1
GGGCACC	5230	0.0	39.75183	94
GTATCAA	16580	0.0	37.012955	1
TACCCCG	335	0.0	35.073883	5
GTACTAG	5705	0.0	33.960133	1
CAACGCA	18570	0.0	31.307362	5
TAGGCAT	7005	0.0	30.863014	5
ATCAACG	18995	0.0	30.715075	3
TATTCTC	3130	0.0	30.631939	5
CTGTGCG	7830	0.0	29.116142	9
TAGTACT	8010	0.0	28.758146	4
TATCAAC	21390	0.0	28.640667	2
ACTGTGC	8375	0.0	27.889355	8
AACGCAG	22025	0.0	27.80352	6
CTAGGCA	8240	0.0	27.327862	4
TGAACCG	570	0.0	27.209951	5
TACTGTG	9570	0.0	27.206274	7
AGGGCTA	6860	0.0	26.719551	5
GTACTGT	8810	0.0	26.35253	6
>>END_MODULE