FastQCFastQC Report
Mon 16 Jan 2017
ERR1146482_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146482_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4553629
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN138850.30492163502999475No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC130670.28695794057882185No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT107620.23633897271824297No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA83030.18233808683140415No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC75360.16549437822009652No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA70210.15418471728812339No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC67730.14873851163544505No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG57090.12537253254492187No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC56480.12403294163841631No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT54460.11959691929228314No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC52430.11513893643948596No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT48600.10672806238716417No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG46770.10270928966764749No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT46080.10119401470782972No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC43250.036.6872494
GTATCAA83100.033.3813861
CAACGCA75450.033.0673145
GTACTAG41300.031.5273231
ATCAACG81200.030.5571983
CTGTGCG59000.028.8866339
TAGTACT60000.028.8183984
GGTATCA51250.028.5361821
TATCAAC97650.028.2979582
GCATCGT41600.027.9886198
TAGGCAT48950.027.6441635
TCTAACG20100.027.5892
CTAACGC19850.027.2253173
AACGCAG98300.026.6963966
TAACGCC20250.026.6836994
ACTGTGC70900.026.4210328
GTACTGT66100.026.1360956
CTAGTAC66350.025.9224363
ACGCAGA96150.025.7814777
TACTGTG72900.025.4385687
Produced by FastQC (version 0.11.5)