##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146482_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4553629
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.54767593056	31.0	31.0	34.0	27.0	34.0
2	30.6576906023745	31.0	31.0	34.0	27.0	34.0
3	30.844549479107762	31.0	31.0	34.0	27.0	34.0
4	34.32237277125563	37.0	35.0	37.0	30.0	37.0
5	34.551417122475286	37.0	35.0	37.0	30.0	37.0
6	34.684479565638746	37.0	35.0	37.0	31.0	37.0
7	34.590700735611094	37.0	35.0	37.0	30.0	37.0
8	34.52449441972546	37.0	35.0	37.0	30.0	37.0
9	35.93630772291726	39.0	35.0	39.0	30.0	39.0
10-11	35.86529545995073	39.0	35.0	39.0	30.0	39.0
12-13	35.87583320907346	39.0	35.0	39.0	30.0	39.0
14-15	36.911866118210334	40.0	36.0	41.0	30.0	41.0
16-17	36.71344558373113	40.0	36.0	41.0	29.5	41.0
18-19	36.64314593920585	40.0	36.0	41.0	28.5	41.0
20-21	36.98807983698276	40.0	36.0	41.0	30.0	41.0
22-23	36.67091247442424	40.0	36.0	41.0	29.0	41.0
24-25	36.78867711884301	40.0	36.0	41.0	30.0	41.0
26-27	36.8658661037164	40.0	36.0	41.0	30.0	41.0
28-29	36.66549393900996	40.0	36.0	41.0	29.5	41.0
30-31	36.5928266224587	39.0	35.5	41.0	29.5	41.0
32-33	36.70760924967756	40.0	35.5	41.0	30.0	41.0
34-35	36.76115489426126	40.0	35.5	41.0	30.0	41.0
36-37	36.68911894227659	40.0	35.0	41.0	30.0	41.0
38-39	36.518064273571696	39.0	35.0	41.0	29.0	41.0
40-41	36.484151651353244	39.0	35.0	41.0	29.0	41.0
42-43	36.31768222663726	39.0	35.0	41.0	29.0	41.0
44-45	36.14445785987396	39.0	35.0	41.0	28.0	41.0
46-47	36.02010561246865	39.0	35.0	41.0	28.0	41.0
48-49	35.89399454808461	38.0	34.0	40.0	28.0	41.0
50-51	35.67933784241096	38.0	34.0	40.0	27.5	41.0
52-53	35.50291240239379	38.0	34.0	40.0	27.0	41.0
54-55	35.301765251407176	38.0	34.0	40.0	27.0	41.0
56-57	35.16863143659705	37.5	33.5	40.0	27.0	41.0
58-59	34.917033315625844	37.0	33.0	40.0	26.5	41.0
60-61	34.652472346780996	36.5	33.0	40.0	26.0	41.0
62-63	34.384684610889465	36.0	33.0	39.0	26.0	41.0
64-65	34.09328032652638	35.5	33.0	39.0	26.0	41.0
66-67	33.79476479089535	35.0	32.0	39.0	26.0	40.5
68-69	33.499571660317514	35.0	32.0	38.0	25.5	40.0
70-71	33.41110452783922	35.0	32.0	37.5	26.0	40.0
72-73	33.19068505580934	35.0	32.0	37.0	25.5	40.0
74-75	32.91943414801689	35.0	32.0	36.5	25.0	39.0
76-77	32.681979032547446	35.0	32.0	36.0	25.0	39.0
78-79	32.46100516752682	35.0	32.0	36.0	25.0	39.0
80-81	32.2283659911688	35.0	31.0	35.0	24.5	37.0
82-83	32.012981075094174	35.0	31.0	35.0	24.0	37.0
84-85	31.840044939980835	35.0	31.0	35.0	24.0	36.5
86-87	31.684191883001446	34.5	31.0	35.0	24.0	36.0
88-89	31.696440575198373	35.0	31.0	35.0	24.0	36.0
90-91	31.636423279103326	35.0	31.5	35.0	24.0	36.0
92-93	31.51173053404219	35.0	31.0	35.0	24.0	35.5
94-95	31.505134915470716	35.0	31.0	35.0	24.0	35.0
96-97	31.435503638965756	35.0	31.0	35.0	24.0	35.0
98-99	31.364531563726423	35.0	31.0	35.0	23.5	35.0
100	30.881985115607794	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7305479546381157
1101	2	0.8985412854464627
1101	3	0.8916984627269073
1101	4	0.8862125795915503
1101	5	0.8756632156337361
1101	6	0.6075733647205581
1101	7	0.6588206293242038
1101	8	0.6906097175514319
1101	9	0.9044949992118987
1101	10-11	0.8712459619291337
1101	12-13	0.7930087922302036
1101	14-15	1.0443919088415043
1101	16-17	1.2013218028120818
1101	18-19	1.328906883428111
1101	20-21	0.9869702526694581
1101	22-23	1.27439036502453
1101	24-25	1.1536615176267588
1101	26-27	1.054333035757196
1101	28-29	1.264901506053576
1101	30-31	1.2924481166652981
1101	32-33	1.110525840814553
1101	34-35	1.0397449375500827
1101	36-37	0.9963913237968285
1101	38-39	1.086253339758926
1101	40-41	1.04570827205157
1101	42-43	1.068664451824489
1101	44-45	1.1288248719657616
1101	46-47	1.042266592962683
1101	48-49	0.9969244908121269
1101	50-51	0.9832794353070042
1101	52-53	1.0043117368525571
1101	54-55	0.9746304565692796
1101	56-57	0.8899862380106214
1101	58-59	0.8423328237279932
1101	60-61	0.9508374901886683
1101	62-63	0.8540530504084174
1101	64-65	0.8885856916607082
1101	66-67	0.9592302197357796
1101	68-69	0.9306128939908973
1101	70-71	0.871281607773426
1101	72-73	0.8322557269596587
1101	74-75	0.8695260984072064
1101	76-77	0.8066437050340767
1101	78-79	0.8377233725225324
1101	80-81	0.8319687774783588
1101	82-83	0.7564276976746598
1101	84-85	0.7521266875111401
1101	86-87	0.8035762004283065
1101	88-89	0.8133502611172965
1101	90-91	0.8608511711357565
1101	92-93	0.8289924964554736
1101	94-95	0.7891300916730444
1101	96-97	0.7779246120025434
1101	98-99	0.7702666077583267
1101	100	0.8561087733118597
1102	1	0.5971881434968331
1102	2	0.758712544495598
1102	3	0.7419464168757628
1102	4	0.8493199143114225
1102	5	0.6269835944181068
1102	6	0.463964757622108
1102	7	0.5404150289496954
1102	8	0.49685003767351077
1102	9	0.6725827312995563
1102	10-11	0.6987054431745463
1102	12-13	0.6125827931762586
1102	14-15	0.8405214871224587
1102	16-17	0.9996243477405429
1102	18-19	1.0435571377692412
1102	20-21	0.8046809574122946
1102	22-23	1.0750971781168985
1102	24-25	0.9091327332885086
1102	26-27	0.7643115843279418
1102	28-29	0.9073112459715134
1102	30-31	0.9637848740049932
1102	32-33	0.7993567942040656
1102	34-35	0.6603114647555515
1102	36-37	0.7092618509866284
1102	38-39	0.6784025718168465
1102	40-41	0.6525304309913409
1102	42-43	0.7416166858100013
1102	44-45	0.8323020541886308
1102	46-47	0.8072353518411219
1102	48-49	0.7367061002859927
1102	50-51	0.8075821965606238
1102	52-53	0.7280966146961418
1102	54-55	0.7678861281915985
1102	56-57	0.6873205810245082
1102	58-59	0.6638675161412735
1102	60-61	0.7607122198128096
1102	62-63	0.7157465387029944
1102	64-65	0.6469651470352034
1102	66-67	0.6718850335456494
1102	68-69	0.6766611300965906
1102	70-71	0.5838624085240127
1102	72-73	0.5599161409972027
1102	74-75	0.5886130496197595
1102	76-77	0.5831698435492285
1102	78-79	0.6062652528531203
1102	80-81	0.6897509282545471
1102	82-83	0.6888688854955518
1102	84-85	0.6356856695201749
1102	86-87	0.5629594143300913
1102	88-89	0.5040162954488814
1102	90-91	0.5200775248259148
1102	92-93	0.6364281843804953
1102	94-95	0.6324346444611493
1102	96-97	0.583683253813355
1102	98-99	0.6183869648122986
1102	100	0.5798505084794527
1103	1	0.5757820166181382
1103	2	0.7901489335335405
1103	3	0.7061859471838474
1103	4	0.7385344275789123
1103	5	0.7145141103734218
1103	6	0.5061747895762423
1103	7	0.6280398120144923
1103	8	0.6453691980677903
1103	9	0.7495009461222111
1103	10-11	0.7807558374044348
1103	12-13	0.7012086204305774
1103	14-15	0.8435882499899208
1103	16-17	1.1030331914456752
1103	18-19	1.1860030792076088
1103	20-21	0.8842618555119657
1103	22-23	1.0872063321783614
1103	24-25	0.9838414116636329
1103	26-27	0.8649568306068005
1103	28-29	1.0669464322578222
1103	30-31	1.1073165187485188
1103	32-33	0.9036727775550517
1103	34-35	0.802298700022078
1103	36-37	0.8107096366763926
1103	38-39	0.8603977956166347
1103	40-41	0.8282808213538573
1103	42-43	0.7984743158865228
1103	44-45	0.8739008001943631
1103	46-47	0.7961106847973909
1103	48-49	0.8377216471077489
1103	50-51	0.7893395771918748
1103	52-53	0.8693321906198364
1103	54-55	0.8591684146765957
1103	56-57	0.7203063950330915
1103	58-59	0.7427604456246328
1103	60-61	0.6894443513613524
1103	62-63	0.5805821271956404
1103	64-65	0.7469138439736938
1103	66-67	0.8119034760596264
1103	68-69	0.7150608975710497
1103	70-71	0.702556724459015
1103	72-73	0.7223861084629633
1103	74-75	0.6763417682155222
1103	76-77	0.6935159005252345
1103	78-79	0.7334614952211069
1103	80-81	0.7237000191812228
1103	82-83	0.7119624540615774
1103	84-85	0.6679874427086752
1103	86-87	0.6202156855099723
1103	88-89	0.6301861744485855
1103	90-91	0.7016444979815546
1103	92-93	0.7367083873206646
1103	94-95	0.7249571477121606
1103	96-97	0.7055579969959069
1103	98-99	0.7101041489812268
1103	100	0.7063945553906343
1104	1	0.5418449600218196
1104	2	0.6501107634249479
1104	3	0.584484036535077
1104	4	0.7205359307133179
1104	5	0.6462974586635468
1104	6	0.3712345794729046
1104	7	0.4000686753634497
1104	8	0.45633422806058377
1104	9	0.5083338642997006
1104	10-11	0.5345569013402951
1104	12-13	0.4496425836266127
1104	14-15	0.6054152641946757
1104	16-17	0.7850807065212777
1104	18-19	0.7197540611839699
1104	20-21	0.6399088369888375
1104	22-23	0.8304821703877963
1104	24-25	0.7082126322777285
1104	26-27	0.5752809550286884
1104	28-29	0.8198757970554027
1104	30-31	0.8422065583630456
1104	32-33	0.6571786778002249
1104	34-35	0.5098728596227105
1104	36-37	0.5543431332764044
1104	38-39	0.6760461889029443
1104	40-41	0.5642369052274532
1104	42-43	0.48346355491658244
1104	44-45	0.6105883923929696
1104	46-47	0.5696494037876718
1104	48-49	0.5107431569330956
1104	50-51	0.5320242609698553
1104	52-53	0.5988794983504917
1104	54-55	0.5539458729723066
1104	56-57	0.4818697549904414
1104	58-59	0.448960374148875
1104	60-61	0.45104266874543697
1104	62-63	0.33392194180596135
1104	64-65	0.37655943088104493
1104	66-67	0.45290524105439545
1104	68-69	0.4683985571917191
1104	70-71	0.3448757006688652
1104	72-73	0.3609922704753217
1104	74-75	0.4007773706015243
1104	76-77	0.4526754918366933
1104	78-79	0.39820662168289545
1104	80-81	0.46844349853198963
1104	82-83	0.4171948970696775
1104	84-85	0.44235875651151346
1104	86-87	0.44218793669836387
1104	88-89	0.37617568046438166
1104	90-91	0.4220769100509827
1104	92-93	0.48135813044590137
1104	94-95	0.4233705977364366
1104	96-97	0.46769150897205947
1104	98-99	0.482427721725756
1104	100	0.5299385278962276
1105	1	0.5037363161681618
1105	2	0.5972230851585749
1105	3	0.5779389034275049
1105	4	0.6732177156457411
1105	5	0.5706963966247898
1105	6	0.3398500218371012
1105	7	0.4084672804791438
1105	8	0.43262739668166716
1105	9	0.4575187579882751
1105	10-11	0.5131089684737731
1105	12-13	0.48059728738717666
1105	14-15	0.663177978477286
1105	16-17	0.8103547007644281
1105	18-19	0.8529655622506667
1105	20-21	0.6398630695951724
1105	22-23	0.9463959489155371
1105	24-25	0.7073643578939368
1105	26-27	0.5342795563128959
1105	28-29	0.8003111353277887
1105	30-31	0.8244640880379492
1105	32-33	0.6369633885232702
1105	34-35	0.5447920804556219
1105	36-37	0.5106098271509438
1105	38-39	0.6636603922339788
1105	40-41	0.5348152791014371
1105	42-43	0.5651621637257875
1105	44-45	0.5668783714327077
1105	46-47	0.5572694425462217
1105	48-49	0.6409354010211601
1105	50-51	0.6783327118518088
1105	52-53	0.6058358594629425
1105	54-55	0.5952059417140987
1105	56-57	0.508494153107371
1105	58-59	0.5561572792733429
1105	60-61	0.5404385343993212
1105	62-63	0.5024547540642672
1105	64-65	0.5122713221636843
1105	66-67	0.5021949145936659
1105	68-69	0.5357084606201425
1105	70-71	0.5050392658925063
1105	72-73	0.5468523669150329
1105	74-75	0.5375206510784736
1105	76-77	0.5283086826536518
1105	78-79	0.5050213967772663
1105	80-81	0.6171316955588182
1105	82-83	0.543589928958589
1105	84-85	0.4840881700449451
1105	86-87	0.6015469480086715
1105	88-89	0.48194568785524794
1105	90-91	0.5176922783866686
1105	92-93	0.610096878107516
1105	94-95	0.4949116723946432
1105	96-97	0.5406488396249571
1105	98-99	0.6757745924969818
1105	100	0.6954383088528253
1106	1	0.48088369227592054
1106	2	0.5310124385479895
1106	3	0.48699886039497287
1106	4	0.6077716141199758
1106	5	0.5153002738954342
1106	6	0.3288057870086334
1106	7	0.3427850189890904
1106	8	0.42045284676117234
1106	9	0.5014492449672332
1106	10-11	0.46954836627888596
1106	12-13	0.36227216659838746
1106	14-15	0.5462188224690507
1106	16-17	0.7000808846204833
1106	18-19	0.6997453965000773
1106	20-21	0.549255324651412
1106	22-23	0.8212320038281646
1106	24-25	0.6236421980508169
1106	26-27	0.4816908188154727
1106	28-29	0.7051070109099626
1106	30-31	0.6542914107209228
1106	32-33	0.5485169762721327
1106	34-35	0.5006775033537139
1106	36-37	0.4572736659489678
1106	38-39	0.547162514655426
1106	40-41	0.5184272736651536
1106	42-43	0.4409004187520509
1106	44-45	0.49436051015258897
1106	46-47	0.541583478842675
1106	48-49	0.5486834281539359
1106	50-51	0.5086830020467161
1106	52-53	0.4313579204211351
1106	54-55	0.5172641096764679
1106	56-57	0.4841841382181826
1106	58-59	0.49505798894750086
1106	60-61	0.45170492375102356
1106	62-63	0.4305662473634797
1106	64-65	0.4205500795141006
1106	66-67	0.4403339671266622
1106	68-69	0.4547581295597496
1106	70-71	0.45109611538917704
1106	72-73	0.38253376853614185
1106	74-75	0.4552303141596781
1106	76-77	0.4182211171619272
1106	78-79	0.4240895984981705
1106	80-81	0.481318089088262
1106	82-83	0.5286864894176944
1106	84-85	0.4280723662618193
1106	86-87	0.4750787163660526
1106	88-89	0.4326779661409752
1106	90-91	0.41002737191804073
1106	92-93	0.44439836379167375
1106	94-95	0.5125468352149483
1106	96-97	0.4646076002237649
1106	98-99	0.4360220480123296
1106	100	0.42251929482718964
1107	1	0.45908773519036217
1107	2	0.5658094869592993
1107	3	0.49998599314666947
1107	4	0.6913515733802171
1107	5	0.5464333223704614
1107	6	0.26188620327594236
1107	7	0.2728001659282313
1107	8	0.3452822996039302
1107	9	0.464213428272231
1107	10-11	0.4098809429289645
1107	12-13	0.326368586810986
1107	14-15	0.556371463458504
1107	16-17	0.7138403394061257
1107	18-19	0.753826623664132
1107	20-21	0.4811289006305941
1107	22-23	0.7608828739021334
1107	24-25	0.4857873835688622
1107	26-27	0.45161971182719185
1107	28-29	0.6149842320471066
1107	30-31	0.6213920891369682
1107	32-33	0.5417458417649286
1107	34-35	0.3818557495238508
1107	36-37	0.3858681088017022
1107	38-39	0.4485563914523567
1107	40-41	0.2978759037023764
1107	42-43	0.43089507634886104
1107	44-45	0.28656585167550475
1107	46-47	0.37487258207974605
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2214	100	-1.1782795456625266
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2215	3	-3.891572333357516
2215	4	-2.0786199421919065
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2215	24-25	-5.493380444458026
2215	26-27	-3.676563341397852
2215	28-29	-1.8887846202009797
2215	30-31	-4.08828796771175
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2215	100	-3.337299274032091
2216	1	-0.45430521130018775
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2216	3	-0.6694681498544028
2216	4	-0.5915189270558301
2216	5	-0.7027180659878596
2216	6	-0.6017913865738223
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2216	8	-0.6261675255149797
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2216	10-11	-0.7301071785873461
2216	12-13	-0.9252083838454439
2216	14-15	-1.2976507875369663
2216	16-17	-1.0200025513285809
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2216	30-31	-0.9309377937754988
2216	32-33	-0.9095603852322043
2216	34-35	-1.0981428604184202
2216	36-37	-1.1135680422340855
2216	38-39	-1.1776325206913043
2216	40-41	-1.2159176906933453
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2216	54-55	-0.8878946368175278
2216	56-57	-0.9787602920811338
2216	58-59	-1.0848281080142428
2216	60-61	-1.0060819035230324
2216	62-63	-1.0711579933775184
2216	64-65	-0.9911549238476951
2216	66-67	-1.0499587456233144
2216	68-69	-1.1764378913045732
2216	70-71	-1.2387445545242741
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2216	74-75	-1.1852045275190264
2216	76-77	-1.046701124423027
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2216	80-81	-1.1975470681657896
2216	82-83	-1.3041743456939443
2216	84-85	-1.2241814529661035
2216	86-87	-1.2419660572957447
2216	88-89	-1.0542083532466187
2216	90-91	-1.1394253978258035
2216	92-93	-1.1056177972331191
2216	94-95	-1.0102907002389294
2216	96-97	-1.0033682873239655
2216	98-99	-1.0442707850136195
2216	100	-1.042410891747945
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	18564.0
1	3129.0
2	494.0
3	499.0
4	453.0
5	254.0
6	258.0
7	353.0
8	656.0
9	1101.0
10	1721.0
11	2949.0
12	4669.0
13	6669.0
14	8766.0
15	11223.0
16	13715.0
17	16530.0
18	18988.0
19	22091.0
20	25723.0
21	29757.0
22	34103.0
23	39796.0
24	45063.0
25	51286.0
26	59005.0
27	68186.0
28	81387.0
29	96710.0
30	116023.0
31	140436.0
32	173465.0
33	220818.0
34	300286.0
35	410200.0
36	606323.0
37	918700.0
38	779923.0
39	223207.0
40	150.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.16692995052393	20.10723438385145	12.55069933249668	24.17513633312794
2	15.517760916542178	19.73060161316082	40.628984481643485	24.12265298865352
3	18.9526340272022	26.128430617085186	28.54214275815744	26.37679259755517
4	12.537096751062627	16.542034526923345	39.293686243837435	31.627182478176596
5	13.576397688416119	38.09735234494397	34.68990337374701	13.6363465928929
6	30.665929020945228	37.61053100340856	18.155463469986103	13.568076505660114
7	27.451879677674953	31.399853889905383	23.15997289679816	17.98829353562151
8	26.136753902480585	34.82345364124106	20.082534561927186	18.95725789435117
9	26.11320414851861	15.505290281735565	21.08298295467715	37.29852261506868
10-11	25.633760402978538	25.593965477653907	27.608184795048956	21.164089324318596
12-13	26.07024014067677	23.279797639160332	27.49598330512034	23.153978915042554
14-15	23.352150653951746	24.643033124547983	24.876437514956866	27.128378706543405
16-17	22.767198358120776	27.2579702939257	26.099472694661863	23.875358653291663
18-19	22.290639483251656	27.655297989668785	27.52034864948551	22.53371387759405
20-21	23.757552476152473	26.8623259556811	26.6398980474362	22.740223520730225
22-23	24.04385224144554	26.489795841800984	26.475671599447963	22.990680317305518
24-25	23.573013285659393	26.598583535602078	26.87811895753289	22.95028422120564
26-27	23.401066560777974	26.883077052455185	26.067987207274356	23.647869179492485
28-29	22.82000348323533	27.286149106753214	26.58927026661319	23.304577143398262
30-31	23.408106117539464	26.928389622719706	26.493271244097734	23.170233015643092
32-33	23.596022520946445	27.090400562471785	25.80376842851167	23.509808488070103
34-35	23.805166082618246	26.97219694013453	26.42472398675556	22.797912990491668
36-37	23.542900410288244	26.54745221280844	26.126487489471188	23.783159887432127
38-39	23.598031924212588	26.67120463225754	26.63222419963987	23.098539243890002
40-41	23.40860065359571	25.96285913291314	27.15648109334036	23.47205912015079
42-43	23.306323985217446	26.15277697277813	26.638448439082683	23.902450602921736
44-45	23.469188341240894	26.848913720819205	26.526582958270783	23.155314979669114
46-47	23.472544414721337	26.652686048525936	26.082295376956115	23.792474159796612
48-49	23.910935251385634	26.358961939992575	26.295748247246664	23.43435456137512
50-51	23.630849937571963	26.363056291211613	26.33874744298957	23.667346328226856
52-53	23.345669664998333	26.612687350655122	26.23116144794616	23.81048153640038
54-55	23.13682340292328	26.600526613685883	26.29673735101018	23.965912632380657
56-57	24.010348206073992	26.249814004274924	26.03773472439738	23.702103065253702
58-59	23.10560658629555	26.460716142628005	26.759519186294888	23.674158084781556
60-61	23.07117640299529	26.94123609003268	26.318293409700246	23.669294097271777
62-63	23.549865782479316	26.405122782541124	25.925295146094008	24.119716288885552
64-65	22.77978635046807	26.542110629181227	26.315792959624005	24.362310060726696
66-67	23.45189611218215	26.238446131251177	26.26465155016533	24.045006206401343
68-69	23.018245473410165	26.30432970477454	26.50560348182657	24.17182133998873
70-71	22.89233167917789	26.036665775452427	26.56789496006205	24.50310758530764
72-73	23.380544398629382	26.117131735972325	26.824221366270134	23.678102499128155
74-75	23.499398524112742	26.453723117527296	26.50656821383464	23.54031014452532
76-77	23.274679866290672	26.282941110762316	26.215675833906204	24.226703189040816
78-79	23.22955831374224	26.3948027765988	26.198133652856253	24.177505256802707
80-81	22.951827078413114	26.658041854989467	26.719504676771017	23.670626389826403
82-83	23.608294763009134	25.843942242204342	27.463542626785337	23.084220368001183
84-85	23.705873014786587	26.251094846675098	26.491017234272128	23.552014904266187
86-87	22.711365920233426	26.77182618228326	26.812930301830697	23.70387759565262
88-89	23.50888190666462	25.704774044956185	26.67891757241097	24.10742647596823
90-91	23.203664045526352	25.911776036271878	26.715814435255837	24.168745482945937
92-93	23.44486080954431	25.969410672293332	26.850690073899937	23.735038444262425
94-95	22.60420598723682	25.910864245777805	27.380417356858324	24.104512410127054
96-97	22.965716893632816	25.74833773344253	26.967479379657316	24.31846599326734
98-99	22.423017335891615	26.309239251822685	26.898084974776125	24.369658437509575
100	23.04918869152295	25.644816348586797	26.326170273653847	24.979824686236405
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	15427.0
1	9037.0
2	2466.5
3	1761.5
4	877.5
5	434.0
6	337.0
7	312.0
8	305.5
9	303.5
10	293.0
11	286.0
12	263.5
13	247.5
14	266.5
15	309.0
16	337.0
17	392.5
18	509.5
19	610.5
20	736.5
21	974.5
22	1331.5
23	1835.0
24	2622.5
25	3858.0
26	5645.0
27	7993.5
28	11711.5
29	18325.0
30	27848.5
31	38704.0
32	53165.0
33	66387.5
34	77257.0
35	89215.0
36	102085.5
37	121995.0
38	133049.0
39	130922.0
40	126726.5
41	123140.0
42	125343.5
43	134356.5
44	148072.0
45	171183.0
46	192217.0
47	206869.5
48	216568.5
49	213188.5
50	205186.0
51	207468.5
52	209003.5
53	201556.0
54	191996.5
55	169782.5
56	149464.0
57	126509.0
58	103993.0
59	96280.0
60	79945.0
61	59186.5
62	45955.0
63	36805.0
64	26730.5
65	15873.0
66	10690.0
67	8318.0
68	6578.0
69	5148.0
70	4071.0
71	3060.5
72	2258.5
73	1836.5
74	1496.5
75	1169.5
76	864.5
77	599.0
78	400.0
79	254.0
80	185.0
81	140.5
82	103.0
83	71.0
84	52.0
85	40.5
86	30.0
87	18.0
88	13.0
89	14.0
90	12.0
91	13.0
92	11.0
93	8.5
94	7.5
95	5.5
96	5.5
97	7.0
98	6.5
99	3.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9923294146273225
2	0.44836766455940963
3	0.4817476346887285
4	0.4660897934372783
5	0.43411529573445706
6	0.4852173947416445
7	0.5006775914331185
8	0.5033567732461297
9	0.5167307218045212
10-11	0.5304999594828652
12-13	0.5291713488296916
14-15	0.5256686480167796
16-17	0.5311148536694579
18-19	0.530379176696213
20-21	0.5286223361630911
22-23	0.49218985560747264
24-25	0.5264043249900244
26-27	0.5069692765923619
28-29	0.5129535146583087
30-31	0.5156875977379799
32-33	0.5187950094309396
34-35	0.5310379918961338
36-37	0.5486503182406823
38-39	0.5365720395754683
40-41	0.5420402057348107
42-43	0.5411398249615856
44-45	0.550132652440504
46-47	0.5485405157073622
48-49	0.521693796310591
50-51	0.5361438096955198
52-53	0.5282490075498026
54-55	0.538087314535286
56-57	0.526063937136732
58-59	0.531971313429355
60-61	0.5252623786434951
62-63	0.5197832322308207
64-65	0.5304560384695371
66-67	0.52774391589653
68-69	0.5483867921607141
70-71	0.532059155456011
72-73	0.5364841975488122
74-75	0.5348151990423463
76-77	0.5227259401238001
78-79	0.5536463335067482
80-81	0.5284027310964508
82-83	0.5275792120965498
84-85	0.5370661509754089
86-87	0.8403517282589337
88-89	1.104360939373849
90-91	1.09503870429497
92-93	1.0944238101083774
94-95	1.0991013980278148
96-97	1.0928316733752355
98-99	1.0884285917890983
100	1.084014529949629
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4553629.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.417662536243554
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.35579779288896	28.196378119637266
2	12.527802719933298	9.375221889901967
3	4.782956580633329	5.3690116577893
4	2.3594348262590747	3.5313814442089004
5	1.3195204887628689	2.468668617909409
6	0.7929003360742611	1.7801086320060486
7	0.5167547230049112	1.3535027687585448
8	0.36724703609385034	1.0993220531196282
9	0.2733637922832142	0.9205770716352981
>10	1.3570449055259	9.244518653101963
>50	0.141813317119126	3.727454410297763
>100	0.1592912813642252	12.99679987200926
>500	0.028641061968125527	7.4852566984304145
>1k	0.016785540381890843	10.488666301623203
>5k	4.6952560508785573E-4	1.134906410869066
>10k+	1.7607210190794589E-4	0.8282253987019091
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	13885	0.30492163502999475	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	13067	0.28695794057882185	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	10762	0.23633897271824297	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	8303	0.18233808683140415	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	7536	0.16549437822009652	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	7021	0.15418471728812339	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	6773	0.14873851163544505	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	5709	0.12537253254492187	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	5648	0.12403294163841631	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	5446	0.11959691929228314	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5243	0.11513893643948596	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	4860	0.10672806238716417	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	4677	0.10270928966764749	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	4608	0.10119401470782972	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.196050666402555E-5
2	0.0	0.0	0.0	0.0	2.196050666402555E-5
3	0.0	0.0	0.0	8.78420266561022E-5	2.196050666402555E-5
4	0.0	0.0	0.0	8.78420266561022E-5	4.39210133280511E-5
5	0.0	0.0	0.0	8.78420266561022E-5	4.39210133280511E-5
6	0.0	0.0	0.0	8.78420266561022E-5	8.78420266561022E-5
7	0.0	0.0	0.0	8.78420266561022E-5	8.78420266561022E-5
8	0.0	0.0	0.0	8.78420266561022E-5	1.0980253332012774E-4
9	0.0	0.0	0.0	1.317630399841533E-4	1.0980253332012774E-4
10-11	0.0	0.0	0.0	1.5372354664817885E-4	1.317630399841533E-4
12-13	0.0	0.0	0.0	1.9764455997622996E-4	2.1960506664025548E-4
14-15	0.0	0.0	0.0	2.4156557330428104E-4	2.4156557330428104E-4
16-17	0.0	0.0	0.0	2.635260799683066E-4	2.4156557330428104E-4
18-19	0.0	0.0	0.0	2.635260799683066E-4	2.635260799683066E-4
20-21	0.0	0.0	0.0	2.745063333003194E-4	2.9646683996434494E-4
22-23	0.0	1.0980253332012775E-5	0.0	3.294075999603832E-4	3.5136810662440875E-4
24-25	0.0	2.196050666402555E-5	0.0	4.941113999405749E-4	3.7332861328843433E-4
26-27	0.0	2.196050666402555E-5	0.0	7.027362132488175E-4	3.952891199524599E-4
28-29	0.0	2.196050666402555E-5	0.0	0.001614097239805878	4.1724962661648544E-4
30-31	0.0	2.196050666402555E-5	0.0	0.005676790972650605	4.3921013328051097E-4
32-33	0.0	2.196050666402555E-5	0.0	0.01649234050468319	4.831311466085621E-4
34-35	0.0	2.196050666402555E-5	0.0	0.03255645112941788	5.050916532725877E-4
36-37	0.0	2.196050666402555E-5	0.0	0.06190666828588802	5.050916532725877E-4
38-39	0.0	2.196050666402555E-5	0.0	0.10981351357345975	5.050916532725877E-4
40-41	0.0	2.196050666402555E-5	0.0	0.16386930072695866	5.050916532725877E-4
42-43	0.0	2.196050666402555E-5	0.0	0.223481096066456	5.160719066046004E-4
44-45	0.0	2.196050666402555E-5	0.0	0.2973123194709099	5.599929199326516E-4
46-47	0.0	2.196050666402555E-5	0.0	0.3923683725661445	6.588151999207665E-4
48-49	0.0	2.196050666402555E-5	0.0	0.5011387620730631	6.697954532527792E-4
50-51	0.0	2.196050666402555E-5	0.0	0.6218886079652075	6.807757065847921E-4
52-53	0.0	2.196050666402555E-5	0.0	0.7445709784437863	7.576374799088815E-4
54-55	0.0	2.196050666402555E-5	0.0	0.8839982352536845	8.454795065649836E-4
56-57	0.0	2.196050666402555E-5	0.0	1.0551364636864355	8.674400132290092E-4
58-59	0.0	2.196050666402555E-5	0.0	1.2582272293153438	8.784202665610219E-4
60-61	0.0	2.196050666402555E-5	0.0	1.4750213511026042	8.894005198930347E-4
62-63	0.0	2.196050666402555E-5	0.0	1.705189421448256	9.443017865530986E-4
64-65	0.0	2.196050666402555E-5	0.0	1.948456934018999	9.77242546549137E-4
66-67	0.0	2.196050666402555E-5	0.0	2.2272785068787995	9.882227998811498E-4
68-69	0.0	2.196050666402555E-5	0.0	2.5453654656538776	9.882227998811498E-4
70-71	0.0	2.196050666402555E-5	0.0	2.897183762664899	0.0010541043198732264
72-73	0.0	2.196050666402555E-5	0.0	3.2709076650732856	0.0011858673598573797
74-75	0.0	3.2940759996038324E-5	0.0	3.6410300443887724	0.0012078278665214053
76-77	0.0	4.39210133280511E-5	0.0	4.060519642684988	0.0012517488798494563
78-79	0.0	4.39210133280511E-5	0.0	4.52155851958954	0.0012517488798494563
80-81	0.0	4.39210133280511E-5	0.0	5.023312175849196	0.0012846896398454946
82-83	0.0	4.39210133280511E-5	0.0	5.552373722145568	0.0013615514131695842
84-85	0.0	4.39210133280511E-5	0.0	6.076527095202529	0.0013615514131695842
86-87	0.0	4.39210133280511E-5	0.0	6.627230720816298	0.0013944921731656225
88	0.0	4.39210133280511E-5	0.0	7.0737427225625975	0.0014274329331616608
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	4325	0.0	36.68724	94
GTATCAA	8310	0.0	33.381386	1
CAACGCA	7545	0.0	33.067314	5
GTACTAG	4130	0.0	31.527323	1
ATCAACG	8120	0.0	30.557198	3
CTGTGCG	5900	0.0	28.886633	9
TAGTACT	6000	0.0	28.818398	4
GGTATCA	5125	0.0	28.536182	1
TATCAAC	9765	0.0	28.297958	2
GCATCGT	4160	0.0	27.988619	8
TAGGCAT	4895	0.0	27.644163	5
TCTAACG	2010	0.0	27.589	2
CTAACGC	1985	0.0	27.225317	3
AACGCAG	9830	0.0	26.696396	6
TAACGCC	2025	0.0	26.683699	4
ACTGTGC	7090	0.0	26.421032	8
GTACTGT	6610	0.0	26.136095	6
CTAGTAC	6635	0.0	25.922436	3
ACGCAGA	9615	0.0	25.781477	7
TACTGTG	7290	0.0	25.438568	7
>>END_MODULE