FastQCFastQC Report
Mon 16 Jan 2017
ERR1146482_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146482_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4553629
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC149650.32863898222714233No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT108350.23794208970471684No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA87230.19156149963029487No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC83670.18374355925790176No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC83150.18260161291137245No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA72660.15956504142080966No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC62660.1376045347567841No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG59630.13095050123758434No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT54590.11988240587891548No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG52800.1159514751860549No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52520.11533658099946219No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG51600.11331621438637184No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC49640.10901195508022284No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA47790.1049492613473781No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT47170.10358770993420853No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT47150.10354378892088047No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT46600.10233596105435906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46360.10180890889442244No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA70200.038.1781121
GTATCAA119300.037.442071
GGGCACC43600.033.661394
CAACGCA132950.032.0269975
ATCAACG137250.031.0331383
TACCCCG3150.029.8397925
TATCAAC152300.029.2648942
GTGGTAT21700.029.2516751
AACGCAG157800.028.0550276
CTGTGCG57250.027.4244529
TAGTACT60000.026.4048544
TAGGCAT51400.026.0590215
GTACTGT63700.025.822336
TCAACGC167850.025.7954654
ACGCAGA167400.025.4635317
TACTGTG70750.025.109167
CTAGTAC64900.024.91863
CGCAGAG171550.024.7653528
ACTGTGC71650.024.4658018
CTAGGCA58650.023.9667024
Produced by FastQC (version 0.11.5)