##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146482_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4553629
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.801075362090323	31.0	31.0	34.0	30.0	34.0
2	31.80712240720533	31.0	31.0	34.0	30.0	34.0
3	31.986984446910366	33.0	31.0	34.0	30.0	34.0
4	35.72017153790966	37.0	35.0	37.0	35.0	37.0
5	35.620079501426225	37.0	35.0	37.0	33.0	37.0
6	35.645956444848714	37.0	35.0	37.0	33.0	37.0
7	35.561086992374655	37.0	35.0	37.0	33.0	37.0
8	35.54021177394996	37.0	35.0	37.0	33.0	37.0
9	37.142027600403985	39.0	37.0	39.0	33.0	39.0
10-11	37.0909152897612	39.0	37.0	39.0	33.0	39.0
12-13	37.031652886082725	39.0	37.0	39.0	33.0	39.0
14-15	38.2328457149232	40.0	37.0	41.0	33.0	41.0
16-17	38.22723656231108	40.0	37.0	41.0	33.0	41.0
18-19	38.27251363692562	40.0	37.5	41.0	33.0	41.0
20-21	38.28300625720716	40.0	38.0	41.0	33.0	41.0
22-23	38.252905649537986	40.0	37.5	41.0	33.0	41.0
24-25	38.189414201288685	40.0	37.0	41.0	33.0	41.0
26-27	38.051703487482186	40.0	37.0	41.0	33.0	41.0
28-29	37.98276627279034	40.0	37.0	41.0	33.0	41.0
30-31	37.87575788453561	40.0	37.0	41.0	33.0	41.0
32-33	37.77777438609952	40.0	37.0	41.0	32.5	41.0
34-35	37.6900769693798	40.0	36.5	41.0	32.0	41.0
36-37	37.61348267502689	40.0	36.0	41.0	32.0	41.0
38-39	37.50896175336199	40.0	36.0	41.0	32.0	41.0
40-41	37.392061474485516	40.0	36.0	41.0	31.0	41.0
42-43	37.27833833191066	39.5	36.0	41.0	31.0	41.0
44-45	37.11627967495815	39.0	35.5	41.0	31.0	41.0
46-47	36.92758511947284	39.0	35.0	41.0	30.5	41.0
48-49	36.716757008531	39.0	35.0	41.0	30.0	41.0
50-51	36.534468113234524	39.0	35.0	41.0	30.0	41.0
52-53	36.34290891945743	38.5	35.0	40.5	30.0	41.0
54-55	36.1380412194318	38.0	35.0	40.0	29.5	41.0
56-57	35.89427059165338	38.0	34.5	40.0	29.0	41.0
58-59	35.633019400570404	37.0	34.0	40.0	29.0	41.0
60-61	35.37318499157485	37.0	34.0	40.0	28.0	41.0
62-63	35.09723629219684	36.0	34.0	39.5	28.0	41.0
64-65	34.819347931067725	36.0	34.0	39.0	28.0	41.0
66-67	34.58390011570991	35.0	33.0	39.0	28.0	41.0
68-69	34.38272880816597	35.0	33.0	39.0	28.0	41.0
70-71	34.1438588870547	35.0	33.0	38.0	27.5	40.0
72-73	33.92204316601111	35.0	33.0	37.0	27.0	40.0
74-75	33.72661497016995	35.0	33.0	37.0	27.0	39.0
76-77	33.55669917334065	35.0	33.0	36.0	27.0	39.0
78-79	33.4285043862818	35.0	33.0	36.0	28.0	39.0
80-81	33.26724728782253	35.0	33.0	36.0	27.5	37.5
82-83	33.0792410844186	35.0	33.0	35.0	27.0	37.0
84-85	32.9332236991639	35.0	33.0	35.0	27.0	37.0
86-87	32.79899493349151	35.0	33.0	35.0	27.0	36.0
88-89	32.688538416282924	35.0	33.0	35.0	27.0	36.0
90-91	32.598384826695366	35.0	33.0	35.0	27.0	36.0
92-93	32.52654091934147	35.0	33.0	35.0	27.0	35.5
94-95	32.48436137419188	35.0	33.0	35.0	27.0	35.0
96-97	32.4309838372867	35.0	33.0	35.0	27.0	35.0
98-99	32.38816546099825	35.0	33.0	35.0	27.0	35.0
100	31.87598308953145	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4835904760713525
1101	2	0.5997385952976408
1101	3	0.5460734153903459
1101	4	0.37734145880753545
1101	5	0.3786551692615916
1101	6	0.417857082035745
1101	7	0.45581586971290733
1101	8	0.44921755168058297
1101	9	0.492102192838189
1101	10-11	0.5256147896161707
1101	12-13	0.5221179735509054
1101	14-15	0.7562064462569822
1101	16-17	0.7609260662663857
1101	18-19	0.7507560059758234
1101	20-21	0.7520035522643127
1101	22-23	0.6824935172838664
1101	24-25	0.737051037727241
1101	26-27	0.7483024851832951
1101	28-29	0.7732008877492973
1101	30-31	0.806682537752458
1101	32-33	0.7712048223621935
1101	34-35	0.7892885427568572
1101	36-37	0.8177878154306271
1101	38-39	0.8370447491890118
1101	40-41	0.8169732904642117
1101	42-43	0.788615711411822
1101	44-45	0.7720989753410592
1101	46-47	0.753214641505167
1101	48-49	0.7737340796965952
1101	50-51	0.7108192265871267
1101	52-53	0.8085906325408843
1101	54-55	0.7774205426766159
1101	56-57	0.7473024424776185
1101	58-59	0.7719100474914882
1101	60-61	0.7819602253935756
1101	62-63	0.7386375921121839
1101	64-65	0.7474731338728802
1101	66-67	0.7184467143273494
1101	68-69	0.726487367132421
1101	70-71	0.6983402969136563
1101	72-73	0.7011311224003833
1101	74-75	0.6042664707687067
1101	76-77	0.6389905256716446
1101	78-79	0.6021677580365363
1101	80-81	0.5918785766134604
1101	82-83	0.6408115415822522
1101	84-85	0.609685073558552
1101	86-87	0.5874605654552525
1101	88-89	0.5400194931864135
1101	90-91	0.551299096350796
1101	92-93	0.6153294354077374
1101	94-95	0.5990115124176256
1101	96-97	0.650120512952526
1101	98-99	0.6086965287160524
1101	100	0.7004245772254833
1102	1	0.42892111953347367
1102	2	0.4765134710391514
1102	3	0.41241084691082364
1102	4	0.2673391854356666
1102	5	0.24233405267138863
1102	6	0.26606066554521135
1102	7	0.3329228437277081
1102	8	0.3457868891554128
1102	9	0.4381657968398116
1102	10-11	0.4042099534763608
1102	12-13	0.4278708279295742
1102	14-15	0.5279778727868134
1102	16-17	0.5150779431685564
1102	18-19	0.5051754629066849
1102	20-21	0.5227482418364886
1102	22-23	0.5520233967070567
1102	24-25	0.5342191358351016
1102	26-27	0.5893702627432802
1102	28-29	0.6342926421634658
1102	30-31	0.6351344963149401
1102	32-33	0.6315943030863806
1102	34-35	0.6162096215146349
1102	36-37	0.6717443267322665
1102	38-39	0.640963795126531
1102	40-41	0.627748522505982
1102	42-43	0.704143587624884
1102	44-45	0.6742644415743939
1102	46-47	0.7425965510934915
1102	48-49	0.7355113939903788
1102	50-51	0.7068567320839705
1102	52-53	0.6797647177656572
1102	54-55	0.661274279950689
1102	56-57	0.6979965795029699
1102	58-59	0.6265277331499917
1102	60-61	0.6635086803939032
1102	62-63	0.6532875160924903
1102	64-65	0.6529291039945591
1102	66-67	0.6181899016142793
1102	68-69	0.617858318040561
1102	70-71	0.5839370423456813
1102	72-73	0.5663547485337332
1102	74-75	0.5997554427127554
1102	76-77	0.5266087489617846
1102	78-79	0.5591401577359321
1102	80-81	0.5284620731083365
1102	82-83	0.5620911832068316
1102	84-85	0.5163766053829448
1102	86-87	0.5474826971830851
1102	88-89	0.4680459225414424
1102	90-91	0.472452454493002
1102	92-93	0.5285598845932284
1102	94-95	0.5228457486148486
1102	96-97	0.5744159328545919
1102	98-99	0.5474292303505592
1102	100	0.5831065246707041
1103	1	0.3143053667743523
1103	2	-0.11871770091856249
1103	3	0.21171979648517691
1103	4	0.21781438835166256
1103	5	0.2483142130705005
1103	6	0.2282739364205426
1103	7	0.32270344307315213
1103	8	0.29995881302626515
1103	9	0.3508495572756658
1103	10-11	0.4017269819836997
1103	12-13	0.4242723018804284
1103	14-15	0.5257946827878328
1103	16-17	0.5814474871574689
1103	18-19	0.5186769300896472
1103	20-21	0.5221287377298225
1103	22-23	0.50878682010174
1103	24-25	0.5291422477132386
1103	26-27	0.5690049414374627
1103	28-29	0.5835672084139105
1103	30-31	0.6293418428650099
1103	32-33	0.6425366599574787
1103	34-35	0.5798023948529973
1103	36-37	0.5207427991216704
1103	38-39	0.5501920012959545
1103	40-41	0.615058115030358
1103	42-43	0.6100477904877053
1103	44-45	0.5775747882356512
1103	46-47	0.5779058076402137
1103	48-49	0.5982195067628453
1103	50-51	0.5799099349902903
1103	52-53	0.5468538944512247
1103	54-55	0.5249654899052985
1103	56-57	0.5545039105835556
1103	58-59	0.4980889173382508
1103	60-61	0.5177580724919011
1103	62-63	0.48354284701838424
1103	64-65	0.503416815430846
1103	66-67	0.49177722438003
1103	68-69	0.487589693035126
1103	70-71	0.46572644962310505
1103	72-73	0.41811706005994154
1103	74-75	0.4433463065378689
1103	76-77	0.45274388207177907
1103	78-79	0.4324295722646667
1103	80-81	0.4146946639069071
1103	82-83	0.41577775231912995
1103	84-85	0.420343558718308
1103	86-87	0.40244888187893935
1103	88-89	0.42461797161835335
1103	90-91	0.399556846856882
1103	92-93	0.38468174779473685
1103	94-95	0.42903432392458996
1103	96-97	0.4156779156544701
1103	98-99	0.37841519180256
1103	100	0.44624627203484835
1104	1	0.34110766033566264
1104	2	0.4406221174193057
1104	3	0.39740111335392214
1104	4	0.25296112800375425
1104	5	0.25051789383941525
1104	6	0.29333209551422357
1104	7	0.35246175341216457
1104	8	0.3705822270703649
1104	9	0.3971930174295579
1104	10-11	0.3480449783139292
1104	12-13	0.4123659374621198
1104	14-15	0.5069841741729917
1104	16-17	0.47215979627197413
1104	18-19	0.4639600421463044
1104	20-21	0.480191614037615
1104	22-23	0.40872221700656297
1104	24-25	0.4182857593073237
1104	26-27	0.505205273003007
1104	28-29	0.5266994427724114
1104	30-31	0.5253642328580241
1104	32-33	0.5208118248782583
1104	34-35	0.4623540726696902
1104	36-37	0.4941437735031542
1104	38-39	0.49263459237163687
1104	40-41	0.48694756802176187
1104	42-43	0.4990093627122434
1104	44-45	0.44714356199294514
1104	46-47	0.3767034515492611
1104	48-49	0.36602630273822
1104	50-51	0.4051104888109833
1104	52-53	0.44843372318895547
1104	54-55	0.4545953162667118
1104	56-57	0.3782073523233578
1104	58-59	0.4767435934095303
1104	60-61	0.3905278795259761
1104	62-63	0.344497521412265
1104	64-65	0.32480452858844444
1104	66-67	0.31107186015649546
1104	68-69	0.37070197102459446
1104	70-71	0.28440140356456567
1104	72-73	0.38839395176810143
1104	74-75	0.3618826072803074
1104	76-77	0.32154486213777744
1104	78-79	0.3072137471389311
1104	80-81	0.33066042310159816
1104	82-83	0.36483645283939836
1104	84-85	0.2754560328700819
1104	86-87	0.32688273697603165
1104	88-89	0.33317713621855205
1104	90-91	0.33336806370615335
1104	92-93	0.34612657167267
1104	94-95	0.30545397286396536
1104	96-97	0.2988507197316608
1104	98-99	0.2855352543135723
1104	100	0.39892109178452273
1105	1	0.10081325985131073
1105	2	-0.4936328802228864
1105	3	-0.02903460367841859
1105	4	0.07942786327363649
1105	5	0.09605098377812737
1105	6	0.2020387320396253
1105	7	0.19174175303399466
1105	8	0.21215681630772565
1105	9	0.25593222265202087
1105	10-11	0.2917295405253455
1105	12-13	0.2564337583386518
1105	14-15	0.3414348486274079
1105	16-17	0.3693649182664487
1105	18-19	0.34526464198537354
1105	20-21	0.38515013059413405
1105	22-23	0.37557841368435874
1105	24-25	0.36386535398440856
1105	26-27	0.34186246282984456
1105	28-29	0.41224624008509636
1105	30-31	0.4214300328671996
1105	32-33	0.45581719516790997
1105	34-35	0.4062372542868147
1105	36-37	0.38863725375868086
1105	38-39	0.39400775893629714
1105	40-41	0.44597285847055446
1105	42-43	0.4301166334988551
1105	44-45	0.41334720859683216
1105	46-47	0.40307262887544226
1105	48-49	0.402586296508332
1105	50-51	0.385340727638976
1105	52-53	0.39635433079537563
1105	54-55	0.3515847875496121
1105	56-57	0.40277207070889887
1105	58-59	0.3572764579605021
1105	60-61	0.31482835030247713
1105	62-63	0.35717636737007297
1105	64-65	0.38317014657526016
1105	66-67	0.3869593896647032
1105	68-69	0.3146336341877074
1105	70-71	0.29416521599320333
1105	72-73	0.2859610399026664
1105	74-75	0.3452031573331311
1105	76-77	0.2886355050320262
1105	78-79	0.29131997643744256
1105	80-81	0.3091191912487332
1105	82-83	0.26211850974345907
1105	84-85	0.26542458240378153
1105	86-87	0.3434160298202471
1105	88-89	0.2564954683565688
1105	90-91	0.19385570324157442
1105	92-93	0.22263290077055586
1105	94-95	0.2676875157652958
1105	96-97	0.23651900141697269
1105	98-99	0.2573026870142954
1105	100	0.31183952727745634
1106	1	0.26469587644986703
1106	2	0.31047323281557127
1106	3	0.2403182559177921
1106	4	0.1851086438782943
1106	5	0.19753319371946532
1106	6	0.25396951780132326
1106	7	0.19964436115110828
1106	8	0.2562248606031332
1106	9	0.28433632110006357
1106	10-11	0.27571693519411156
1106	12-13	0.29237318578319815
1106	14-15	0.365266018572477
1106	16-17	0.3694283553146036
1106	18-19	0.3794076707607985
1106	20-21	0.41836409920848183
1106	22-23	0.3022141352023411
1106	24-25	0.2947299804310646
1106	26-27	0.3226658019007118
1106	28-29	0.3652851602286802
1106	30-31	0.3382827475233867
1106	32-33	0.37491697970126125
1106	34-35	0.35785501301236167
1106	36-37	0.3588675922365354
1106	38-39	0.4061372091651947
1106	40-41	0.36097878744060097
1106	42-43	0.3648228112880503
1106	44-45	0.36906431649279625
1106	46-47	0.3372989520259111
1106	48-49	0.36445887174848934
1106	50-51	0.389456286207448
1106	52-53	0.37198205140659013
1106	54-55	0.3244837271097296
1106	56-57	0.332711860340666
1106	58-59	0.37666478715047447
1106	60-61	0.36637205390131555
1106	62-63	0.36525587832178275
1106	64-65	0.3034014947476962
1106	66-67	0.3080676955860966
1106	68-69	0.30580106478422664
1106	70-71	0.3177038242171619
1106	72-73	0.29461848700824333
1106	74-75	0.24041871409935567
1106	76-77	0.27684398763911844
1106	78-79	0.28342194994522885
1106	80-81	0.267932695881278
1106	82-83	0.26909372966688494
1106	84-85	0.2638547461719014
1106	86-87	0.33820758000109663
1106	88-89	0.2907647415262957
1106	90-91	0.27563186676258766
1106	92-93	0.2553873714919135
1106	94-95	0.26116805895058093
1106	96-97	0.24321589305589697
1106	98-99	0.26129382515289024
1106	100	0.3117143468624093
1107	1	0.14895957529174453
1107	2	-0.49995979526652334
1107	3	0.017549893424103402
1107	4	0.06267683811457658
1107	5	0.09513221418806239
1107	6	0.184879354844405
1107	7	0.19873598493777678
1107	8	0.18270083833732542
1107	9	0.18746585017004946
1107	10-11	0.24813699353927632
1107	12-13	0.16407445092044526
1107	14-15	0.21164349614077338
1107	16-17	0.29907157751518554
1107	18-19	0.3012133736976139
1107	20-21	0.26196428163405727
1107	22-23	0.2623734725347546
1107	24-25	0.23851792244471426
1107	26-27	0.3178843196049357
1107	28-29	0.2740274088711203
1107	30-31	0.27465705192634715
1107	32-33	0.2414063312584247
1107	34-35	0.3123641249641125
1107	36-37	0.3192740969320411
1107	38-39	0.36470952369587906
1107	40-41	0.3772437538719089
1107	42-43	0.4177890397127797
1107	44-45	0.309369919141659
1107	46-47	0.3511820743283849
1107	48-49	0.39790679044335775
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2216	80-81	-0.6059197570116197
2216	82-83	-0.5335250085500931
2216	84-85	-0.6097109755830274
2216	86-87	-0.6183702111933869
2216	88-89	-0.5876375565240011
2216	90-91	-0.5948469552647211
2216	92-93	-0.6555782226349578
2216	94-95	-0.5661033993413511
2216	96-97	-0.5704904092863821
2216	98-99	-0.6325318333029912
2216	100	-0.6692160673900567
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	5.0
13	37.0
14	211.0
15	566.0
16	1523.0
17	3042.0
18	5257.0
19	8305.0
20	12123.0
21	17589.0
22	24506.0
23	29435.0
24	33156.0
25	37572.0
26	45723.0
27	56386.0
28	71632.0
29	89792.0
30	112043.0
31	137004.0
32	170168.0
33	214856.0
34	289285.0
35	397532.0
36	596156.0
37	1000996.0
38	904174.0
39	294121.0
40	431.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.46597949918625	20.74348944320106	13.73583073979956	25.05470031781313
2	15.004222096553693	20.249789993246843	39.6823490010267	25.063638909172763
3	18.31417680054498	25.80898633106828	29.37865018365939	26.498186684727347
4	12.704231397559033	17.167178524255373	38.0041793587652	32.124410719420396
5	13.238569556854971	38.25300900538443	34.69128568849706	13.817135749263535
6	30.593445359733963	38.09559364629837	17.606792296869155	13.704168697098511
7	26.804183933187076	31.84447065075841	23.239254130239527	18.11209128581499
8	26.156215185734276	34.89882465172284	19.7766001578082	19.168360004734687
9	25.89062481813955	15.14168150281896	20.980167686036786	37.9875259930047
10-11	25.59560737161504	25.704959714548547	27.385278862199797	21.31415405163662
12-13	26.0277023007364	23.34020843595295	27.723075375705836	22.90901388760481
14-15	23.22615076717812	24.598182052781414	25.317135978169258	26.858531201871205
16-17	22.40594103002172	27.2375464383171	26.40990590397051	23.946606627690674
18-19	22.02564152679105	27.120984164498253	28.516047310837138	22.337326997873564
20-21	23.22644635286253	26.33618790827354	27.955908104881694	22.481457633982238
22-23	23.48962735197849	25.946470985013683	27.755319900039233	22.808581762968597
24-25	23.1152889267	26.03989038193494	28.22672861579193	22.61809207557313
26-27	23.003139811369753	26.343187586572654	27.420419040466175	23.233253561591415
28-29	22.425169024529666	26.78842523183158	27.869848421994853	22.9165573216439
30-31	23.11246700159367	26.36227061976283	27.724502808639002	22.800759570004494
32-33	23.299449735584517	26.546123981554054	27.134456935336633	23.0199693475248
34-35	23.399301321647332	26.415740521642505	27.717392676888984	22.467565479821175
36-37	23.23361213660577	25.983298156261743	27.381282049986943	23.401807657145543
38-39	23.271702635414524	26.07882636025025	27.886296841486207	22.763174162849015
40-41	23.042134080111452	25.4674537059201	28.322026543359264	23.168385670609183
42-43	23.116547745922837	25.63779854721316	27.623044044559975	23.622609662304033
44-45	23.261128651455795	26.284080235785567	27.45097371788523	23.00381739487341
46-47	23.341954296232743	26.17957018457147	26.870272040168402	23.608203479027388
48-49	23.9082181030633	26.006020072691943	26.91223723122774	23.17352459301702
50-51	23.510984316025745	26.091552473862055	26.765169055274374	23.632294154837822
52-53	23.151413960162323	26.344361826578318	26.676459588605045	23.827764624654314
54-55	23.03090208906871	26.348952303316402	26.62941122731689	23.990734380297994
56-57	23.91183851269133	25.93212083306889	26.422148439670316	23.73389221456946
58-59	22.913707938107223	26.211295328526752	27.167436347852707	23.707560385513318
60-61	22.954088550779712	26.720088579987216	26.633168808714903	23.692654060518166
62-63	23.42318621038297	26.111997705566264	26.31048774504906	24.154328339001708
64-65	22.636505960410915	26.273330567773527	26.666269913512934	24.42389355830262
66-67	23.20442662324928	26.00826725233874	26.675921556191778	24.111384568220203
68-69	22.883455704607417	25.909691987182338	26.936218372414288	24.270633935795953
70-71	22.71959145999599	25.688211102426862	26.964283714289916	24.627913723287232
72-73	23.14194898179013	25.750582667143064	27.201502362181902	23.90596598888491
74-75	23.259611344082227	26.11848187039906	26.814104789017694	23.807801996501023
76-77	23.012984953775128	26.035845825003662	26.46834820064825	24.482821020572963
78-79	22.877916673895374	26.12415926465263	26.509173065811844	24.488750995640153
80-81	22.627544384425683	26.523847072246998	26.90891489998811	23.939693643339215
82-83	23.288045471455384	25.608307210788624	27.680344382763465	23.423302934992527
84-85	23.380375569223133	26.022328590676548	26.648822681297634	23.948473158802685
86-87	22.320300654123013	26.554332796283642	26.939407529354714	24.18595902023863
88-89	22.980736033987018	25.56322184417873	26.83188206800039	24.62416005383386
90-91	22.645511035133133	25.68890794194888	26.917273946350896	24.748307076567094
92-93	22.911191156869116	25.835160058938396	26.822140946567806	24.431507837624682
94-95	21.984821288276603	25.701417257332388	27.459189245486236	24.85457220890477
96-97	22.26640772485815	25.58045585236891	27.068167024054972	25.08496939871797
98-99	21.765551830614406	26.130255376065147	26.957688628812814	25.146504164507633
100	22.267951511714926	25.424876894657434	26.523676555753728	25.783495037873916
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	90.0
1	61.5
2	70.0
3	104.5
4	74.0
5	53.0
6	131.5
7	334.5
8	536.0
9	576.5
10	448.5
11	328.5
12	346.5
13	409.0
14	496.5
15	646.0
16	802.0
17	1073.5
18	1481.0
19	1949.5
20	2427.0
21	2980.5
22	3701.5
23	4512.0
24	5362.5
25	6552.5
26	8354.5
27	10611.0
28	14201.0
29	20865.5
30	30392.0
31	40807.5
32	55093.0
33	67683.5
34	77505.0
35	88632.0
36	100178.0
37	120292.0
38	131943.5
39	129227.0
40	124940.5
41	121493.0
42	123877.5
43	132458.5
44	145359.5
45	169543.5
46	192502.5
47	207853.5
48	218453.0
49	214051.0
50	204751.5
51	206586.5
52	207032.0
53	200636.5
54	194271.5
55	172212.0
56	151211.5
57	127453.0
58	103654.5
59	98054.5
60	81586.5
61	59380.0
62	45863.5
63	36043.5
64	25556.0
65	14717.0
66	9968.5
67	7889.0
68	6216.5
69	4783.5
70	3583.0
71	2672.5
72	1963.0
73	1591.0
74	1263.5
75	996.0
76	756.0
77	480.5
78	287.0
79	158.5
80	97.0
81	47.0
82	26.0
83	10.5
84	3.0
85	2.0
86	2.0
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014032763758312328
2	0.004479943359461212
3	0.001998406106426325
4	0.027582396370016093
5	0.002876826372987347
6	0.0
7	4.172496266164855E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009146551025566642
16-17	0.003129372199623641
18-19	0.0
20-21	6.588151999207665E-5
22-23	9.333215332210859E-4
24-25	0.0
26-27	2.5254582663629383E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	6.917559599168049E-4
36-37	0.0
38-39	0.0
40-41	5.050916532725877E-4
42-43	7.905782399049197E-4
44-45	0.0
46-47	0.0
48-49	6.478349465887537E-4
50-51	0.0
52-53	0.0
54-55	4.611706399445365E-4
56-57	1.756840533122044E-4
58-59	2.635260799683066E-4
60-61	9.882227998811497E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0015042947064857503
70-71	0.0010541043198732264
72-73	0.0
74-75	2.7450633330031935E-4
76-77	0.0028109448529952705
78-79	5.709731732646642E-4
80-81	0.0031952537196157176
82-83	0.001998406106426325
84-85	0.006017178825943001
86-87	0.00783990087905712
88-89	0.007927742905713223
90-91	0.00577561325263872
92-93	0.00878420266561022
94-95	0.0017678207864540569
96-97	0.004941113999405749
98-99	0.001965465346430287
100	0.08046329641698961
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4553629.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.706624867558784
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.64886029573424	21.079748006865618
2	15.62350702968839	9.59490338952618
3	6.159796070129547	5.6743964155839235
4	3.004205983816535	3.689961046797189
5	1.6961248649670908	2.6041134978541627
6	1.0625633740685714	1.9576640955318696
7	0.6956649453515427	1.495306575729442
8	0.4850970526663111	1.1916554576466258
9	0.36529343468485825	1.0095235618905025
>10	1.7934285961143652	10.03466257627462
>50	0.19262763169784672	4.168810794821201
>100	0.2082072111380164	14.11133496137544
>500	0.03907468043505929	8.411135194920263
>1k	0.024690045322658564	12.958330852860959
>5k	7.156534876132918E-4	1.451490750296042
>10k+	1.4313069752265836E-4	0.5669628220259781
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	14965	0.32863898222714233	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	10835	0.23794208970471684	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	8723	0.19156149963029487	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	8367	0.18374355925790176	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	8315	0.18260161291137245	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	7266	0.15956504142080966	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	6266	0.1376045347567841	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	5963	0.13095050123758434	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	5459	0.11988240587891548	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	5280	0.1159514751860549	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5252	0.11533658099946219	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	5160	0.11331621438637184	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	4964	0.10901195508022284	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4779	0.1049492613473781	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	4717	0.10358770993420853	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	4715	0.10354378892088047	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	4660	0.10233596105435906	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4636	0.10180890889442244	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.196050666402555E-5
2	0.0	0.0	0.0	0.0	2.196050666402555E-5
3	0.0	0.0	0.0	6.588151999207665E-5	2.196050666402555E-5
4	0.0	0.0	0.0	6.588151999207665E-5	2.196050666402555E-5
5	0.0	0.0	0.0	6.588151999207665E-5	2.196050666402555E-5
6	0.0	0.0	0.0	6.588151999207665E-5	6.588151999207665E-5
7	0.0	0.0	0.0	6.588151999207665E-5	6.588151999207665E-5
8	0.0	0.0	0.0	8.78420266561022E-5	8.78420266561022E-5
9	0.0	0.0	0.0	1.0980253332012774E-4	1.0980253332012774E-4
10-11	0.0	0.0	0.0	1.0980253332012774E-4	1.0980253332012774E-4
12-13	0.0	0.0	0.0	1.0980253332012774E-4	2.8548658663233217E-4
14-15	0.0	0.0	0.0	1.0980253332012774E-4	3.7332861328843433E-4
16-17	0.0	0.0	0.0	1.2078278665214052E-4	4.3921013328051097E-4
18-19	0.0	0.0	0.0	1.317630399841533E-4	4.6117063994453655E-4
20-21	0.0	0.0	0.0	1.4274329331616609E-4	5.160719066046004E-4
22-23	0.0	0.0	0.0	1.866643066442172E-4	5.490126666006388E-4
24-25	0.0	0.0	0.0	3.5136810662440875E-4	5.709731732646643E-4
26-27	0.0	0.0	0.0	5.709731732646643E-4	5.819534265966771E-4
28-29	0.0	0.0	0.0	0.0014274329331616608	6.368546932567409E-4
30-31	0.0	0.0	0.0	0.005512087172670413	6.588151999207665E-4
32-33	0.0	0.0	0.0	0.0165033207580152	7.027362132488176E-4
34-35	0.0	0.0	0.0	0.03278703644939014	7.905782399049198E-4
36-37	0.0	0.0	0.0	0.0626533255124649	8.015584932369326E-4
38-39	0.0	0.0	0.0	0.11132878853327752	8.564597598969964E-4
40-41	0.0	0.0	0.0	0.16619711443334537	8.784202665610219E-4
42-43	0.0	0.0	0.0	0.2270935994126882	9.113610265570602E-4
44-45	0.0	0.0	0.0	0.3023632360036358	9.443017865530986E-4
46-47	0.0	0.0	0.0	0.3987039787387159	9.662622932171242E-4
48-49	0.0	0.0	0.0	0.5096155176453769	0.0011199858398653032
50-51	0.0	0.0	0.0	0.6320892633106474	0.0011639068531933542
52-53	0.0	0.0	0.0	0.7560343629224076	0.0012737093865134818
54-55	0.0	0.0	0.0	0.8968670921588034	0.0013725316665015967
56-57	0.0	0.0	0.0	1.0697292203646804	0.0014384131864936735
58-59	0.0	0.0	0.0	1.275510148059932	0.00150429470648575
60-61	0.0	0.0	0.0	1.4944234587402707	0.0015372354664817884
62-63	0.0	0.0	0.0	1.7269303230456412	0.001570176226477827
64-65	0.0	0.0	0.0	1.9727891754027391	0.0015811564798098397
66-67	0.0	0.0	0.0	2.255256192368768	0.0015921367331418524
68-69	0.0	0.0	0.0	2.575780767383553	0.0016031169864738652
70-71	0.0	0.0	0.0	2.931134705967482	0.001614097239805878
72-73	0.0	0.0	0.0	3.3094812950286463	0.0017238997731260056
74-75	0.0	0.0	0.0	3.6852365443034554	0.0017568405331220439
76-77	0.0	0.0	0.0	4.1131040758919974	0.0018007615464500951
78-79	0.0	0.0	0.0	4.584640074981953	0.001855662813110159
80-81	0.0	0.0	0.0	5.096166156707101	0.0018886035731061972
82-83	0.0	0.0	0.0	5.635834627722197	0.002009386359758338
84-85	0.0	0.0	0.0	6.172889798444274	0.0020203666130903506
86-87	0.0	0.0	0.0	6.73569366322992	0.0021082086397464527
88	0.0	0.0	0.0	7.190199289401925	0.0021740901597385293
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	7020	0.0	38.178112	1
GTATCAA	11930	0.0	37.44207	1
GGGCACC	4360	0.0	33.6613	94
CAACGCA	13295	0.0	32.026997	5
ATCAACG	13725	0.0	31.033138	3
TACCCCG	315	0.0	29.839792	5
TATCAAC	15230	0.0	29.264894	2
GTGGTAT	2170	0.0	29.251675	1
AACGCAG	15780	0.0	28.055027	6
CTGTGCG	5725	0.0	27.424452	9
TAGTACT	6000	0.0	26.404854	4
TAGGCAT	5140	0.0	26.059021	5
GTACTGT	6370	0.0	25.82233	6
TCAACGC	16785	0.0	25.795465	4
ACGCAGA	16740	0.0	25.463531	7
TACTGTG	7075	0.0	25.10916	7
CTAGTAC	6490	0.0	24.9186	3
CGCAGAG	17155	0.0	24.765352	8
ACTGTGC	7165	0.0	24.465801	8
CTAGGCA	5865	0.0	23.966702	4
>>END_MODULE