FastQCFastQC Report
Mon 16 Jan 2017
ERR1146481_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146481_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3884414
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN121350.3124023340457531No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC81700.21032773540616423No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT69660.17933206913578212No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA66420.1709910426643504No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC57670.14846512240971224No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA54360.1399438885762434No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC51360.13222071591751033No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC44470.11448316271128668No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG28100.032.5700841
TAGGCAT35850.027.520745
GCATCGT29800.026.9394768
GGGCACC30150.026.15359994
GGTATCA60200.025.7823731
GTATCAA85250.025.3451351
CTAACGC13600.025.2237473
GTACATG147850.025.1436521
CTGTGCG35900.024.8489389
TACATGG147350.024.748482
ACATGGG144750.024.4456773
CAACGCA79050.024.3081235
TCTAACG14000.023.8323732
TAACGCC14500.023.6547644
CTAGGCA42000.023.6046164
TAGTACT38100.023.5544054
ATCAACG82850.023.4251943
CATGGGG112200.023.1577344
GCTATTG33700.023.1253058
CGCCTAC15250.022.4719037
Produced by FastQC (version 0.11.5)