##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146481_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3884414
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.473312834316836	31.0	30.0	34.0	27.0	34.0
2	30.57975076806952	31.0	31.0	34.0	26.0	34.0
3	30.79649491532056	31.0	31.0	34.0	27.0	34.0
4	34.236910123380255	37.0	35.0	37.0	30.0	37.0
5	34.48102313502114	37.0	35.0	37.0	30.0	37.0
6	34.60123431745432	37.0	35.0	37.0	31.0	37.0
7	34.51028675110326	37.0	35.0	37.0	30.0	37.0
8	34.43111522098314	37.0	35.0	37.0	30.0	37.0
9	35.83908692533803	39.0	35.0	39.0	30.0	39.0
10-11	35.76254925453363	39.0	35.0	39.0	30.0	39.0
12-13	35.74935665971753	39.0	35.0	39.0	30.0	39.0
14-15	36.786415402683645	40.0	36.0	41.0	29.5	41.0
16-17	36.61770333955134	40.0	36.0	41.0	28.5	41.0
18-19	36.5725966645162	40.0	36.0	41.0	28.0	41.0
20-21	36.90103217628193	40.0	36.0	41.0	30.0	41.0
22-23	36.63504778326924	40.0	36.0	41.0	28.5	41.0
24-25	36.756624422628484	40.0	36.0	41.0	29.5	41.0
26-27	36.83446306186725	40.0	36.0	41.0	30.0	41.0
28-29	36.66693521854262	40.0	36.0	41.0	29.5	41.0
30-31	36.60834980514436	40.0	36.0	41.0	29.5	41.0
32-33	36.7007279605109	40.0	36.0	41.0	30.0	41.0
34-35	36.74221169525185	40.0	36.0	41.0	30.0	41.0
36-37	36.66974658210994	40.0	35.0	41.0	30.0	41.0
38-39	36.51640839519166	39.0	35.0	41.0	29.0	41.0
40-41	36.48286112139437	39.0	35.0	41.0	29.0	41.0
42-43	36.32671478889738	39.0	35.0	41.0	28.5	41.0
44-45	36.17386354801522	39.0	35.0	41.0	28.0	41.0
46-47	36.05502567440031	39.0	35.0	41.0	28.0	41.0
48-49	35.94869470658895	39.0	34.5	41.0	28.0	41.0
50-51	35.75839019733736	38.5	34.0	40.0	27.5	41.0
52-53	35.59971002061057	38.0	34.0	40.0	27.0	41.0
54-55	35.416180278415226	38.0	34.0	40.0	27.0	41.0
56-57	35.292864895451416	38.0	34.0	40.0	27.0	41.0
58-59	35.075712707244904	37.0	33.0	40.0	26.5	41.0
60-61	34.82405003689102	37.0	33.0	40.0	26.0	41.0
62-63	34.57113749976187	36.0	33.0	40.0	26.0	41.0
64-65	34.30024503052455	36.0	33.0	39.0	26.0	41.0
66-67	34.003973186174285	35.5	32.5	39.0	26.0	41.0
68-69	33.72079224820011	35.0	32.0	39.0	26.0	40.5
70-71	33.60623687382447	35.0	32.0	38.5	26.0	40.0
72-73	33.37391779043119	35.0	32.0	37.5	25.5	40.0
74-75	33.09690251862958	35.0	32.0	37.0	25.0	39.5
76-77	32.84260598895998	35.0	32.0	36.5	25.0	39.0
78-79	32.60612527397955	35.0	32.0	36.0	25.0	39.0
80-81	32.34826141085888	35.0	31.5	36.0	24.5	38.0
82-83	32.11220534165514	35.0	31.0	35.0	24.0	37.0
84-85	31.910422267039507	35.0	31.0	35.0	24.0	37.0
86-87	31.72930678861728	35.0	31.0	35.0	24.0	36.0
88-89	31.696243757745698	35.0	31.0	35.0	24.0	36.0
90-91	31.613770313874888	35.0	31.0	35.0	24.0	36.0
92-93	31.476423342105143	35.0	31.0	35.0	23.5	36.0
94-95	31.434538645983665	35.0	31.0	35.0	24.0	35.0
96-97	31.34845088602811	35.0	31.0	35.0	23.0	35.0
98-99	31.263423775117687	35.0	31.0	35.0	23.0	35.0
100	30.788087212125173	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.9128025689791244
1101	2	0.9940030109875231
1101	3	0.945684503334391
1101	4	0.9845306421455575
1101	5	0.9339964424323526
1101	6	0.7164634167324806
1101	7	0.8135509324095764
1101	8	0.850405799327774
1101	9	0.9671707872827255
1101	10-11	1.0483084469190445
1101	12-13	0.9499631631051599
1101	14-15	1.1847382054277844
1101	16-17	1.2569295235350566
1101	18-19	1.4298875117994214
1101	20-21	1.1029480247679828
1101	22-23	1.3953643738024653
1101	24-25	1.2786089862922267
1101	26-27	1.1479152043776821
1101	28-29	1.355576072129935
1101	30-31	1.3397517152748364
1101	32-33	1.1512706936017025
1101	34-35	1.0952489757193362
1101	36-37	1.0755399279646554
1101	38-39	1.1701589516316133
1101	40-41	1.0456962675262744
1101	42-43	1.025754616364118
1101	44-45	1.1114135138087775
1101	46-47	1.047345283769296
1101	48-49	1.04610504890627
1101	50-51	1.0336783272806613
1101	52-53	1.098950791017998
1101	54-55	1.1100361840174529
1101	56-57	1.0053792762938514
1101	58-59	0.9237433717010219
1101	60-61	0.9287222575220113
1101	62-63	0.80770883651563
1101	64-65	0.7971572055610565
1101	66-67	0.9498359919618053
1101	68-69	0.8277995778737761
1101	70-71	0.7266946348645931
1101	72-73	0.7442287660942242
1101	74-75	0.6776086859892487
1101	76-77	0.7388199402162243
1101	78-79	0.7460187547380102
1101	80-81	0.7539124193796098
1101	82-83	0.7674723132357286
1101	84-85	0.7425482328616937
1101	86-87	0.744248727062157
1101	88-89	0.7494734748954528
1101	90-91	0.74575609937229
1101	92-93	0.7890244695683002
1101	94-95	0.7800181531825352
1101	96-97	0.6976376392082919
1101	98-99	0.7662906416659467
1101	100	0.8266542619308872
1102	1	0.738977607243239
1102	2	0.9486605719547327
1102	3	0.9165176883775459
1102	4	0.9677340627827036
1102	5	0.8227129297702618
1102	6	0.6648575679368136
1102	7	0.7707141774837467
1102	8	0.7244010086888366
1102	9	0.8584267111473451
1102	10-11	0.8880427586841151
1102	12-13	0.8984127459661977
1102	14-15	1.1091170244248403
1102	16-17	1.2856719005011925
1102	18-19	1.2643228905083959
1102	20-21	1.0644377998844732
1102	22-23	1.3167475736851344
1102	24-25	1.2082052968535422
1102	26-27	1.0221290827007863
1102	28-29	1.178844071696986
1102	30-31	1.1933733892835718
1102	32-33	1.0428842723839011
1102	34-35	0.9774971897543949
1102	36-37	0.8994914796468549
1102	38-39	0.9839040707850089
1102	40-41	0.9284310701974476
1102	42-43	0.9932623744339111
1102	44-45	1.0483216981984071
1102	46-47	0.9477435203287214
1102	48-49	0.8948954944623608
1102	50-51	0.9854158315102168
1102	52-53	0.9531434347122314
1102	54-55	0.9326304737379374
1102	56-57	0.7995322837289365
1102	58-59	0.8490857619161574
1102	60-61	0.9349841450140133
1102	62-63	0.8821459532380089
1102	64-65	0.902224577595554
1102	66-67	0.9425086723333251
1102	68-69	0.9259676639792929
1102	70-71	0.7854075989328138
1102	72-73	0.7283937107575724
1102	74-75	0.7349237460879721
1102	76-77	0.769074732666148
1102	78-79	0.7401723684568395
1102	80-81	0.8101372579480284
1102	82-83	0.798188445622074
1102	84-85	0.7588399694609649
1102	86-87	0.7586580444937425
1102	88-89	0.7894764700946411
1102	90-91	0.7833278180150565
1102	92-93	0.8592894223889296
1102	94-95	0.811527598663357
1102	96-97	0.7693595102408715
1102	98-99	0.7900858351324089
1102	100	0.7743394548440783
1103	1	0.6556554579709193
1103	2	0.8129701753947707
1103	3	0.7833361003840373
1103	4	0.7998639911510281
1103	5	0.7166594001700801
1103	6	0.6155278063452059
1103	7	0.6402615003774272
1103	8	0.6329994937406624
1103	9	0.7364739204109654
1103	10-11	0.8174203343733595
1103	12-13	0.7036365077116997
1103	14-15	0.8952715147806103
1103	16-17	1.0777342592465047
1103	18-19	1.1736416290894738
1103	20-21	0.8131945796622659
1103	22-23	1.0610745705768636
1103	24-25	0.993143856095962
1103	26-27	0.8798535110163215
1103	28-29	1.0599852395992713
1103	30-31	1.0937314581809687
1103	32-33	0.8833351421459525
1103	34-35	0.8856805224614277
1103	36-37	0.8874225357264152
1103	38-39	0.9474860839517802
1103	40-41	0.8814517508683508
1103	42-43	0.9391404663794205
1103	44-45	0.9269558469839936
1103	46-47	0.8449602101687645
1103	48-49	0.8389850313706546
1103	50-51	0.9089329592286077
1103	52-53	0.9080269325854715
1103	54-55	0.94435047495476
1103	56-57	0.7429480928018037
1103	58-59	0.8743966340760778
1103	60-61	0.8198080005266846
1103	62-63	0.7219257793392799
1103	64-65	0.7688264661808333
1103	66-67	0.7442779013131045
1103	68-69	0.8035183009339377
1103	70-71	0.670926655678052
1103	72-73	0.7062447963272689
1103	74-75	0.7362729912085868
1103	76-77	0.730100516847763
1103	78-79	0.6580598872236934
1103	80-81	0.7008448242784553
1103	82-83	0.6751056173609271
1103	84-85	0.6523149198484646
1103	86-87	0.7172313877764225
1103	88-89	0.6880110896844585
1103	90-91	0.7340633713397686
1103	92-93	0.7622466670657637
1103	94-95	0.7430337248446115
1103	96-97	0.7398953251831308
1103	98-99	0.7172614928894063
1103	100	0.8186014947094407
1104	1	0.6451393635574085
1104	2	0.7480469174665387
1104	3	0.7074761283011703
1104	4	0.7589587213264721
1104	5	0.6769685196266835
1104	6	0.3468761750316247
1104	7	0.40213244102137224
1104	8	0.40003624569099117
1104	9	0.6329113312921635
1104	10-11	0.5992737171475468
1104	12-13	0.5011925267501454
1104	14-15	0.6614607179525578
1104	16-17	0.8081894118683763
1104	18-19	0.8166051715729665
1104	20-21	0.6543953048106985
1104	22-23	0.9050363638831129
1104	24-25	0.7973441648308537
1104	26-27	0.669879535360586
1104	28-29	0.8293648435076619
1104	30-31	0.9080960429601816
1104	32-33	0.8056902401473565
1104	34-35	0.598073362071311
1104	36-37	0.624122990176005
1104	38-39	0.7209411927090201
1104	40-41	0.6633901589187658
1104	42-43	0.5991309964263891
1104	44-45	0.6535247736405339
1104	46-47	0.6478241498975592
1104	48-49	0.6019468793574774
1104	50-51	0.6923155952044553
1104	52-53	0.6839546845351947
1104	54-55	0.6154997462911425
1104	56-57	0.5275934893290497
1104	58-59	0.5221396185894562
1104	60-61	0.5173523623894525
1104	62-63	0.4475819611575744
1104	64-65	0.5332370487331701
1104	66-67	0.6479028022421289
1104	68-69	0.5392110706608548
1104	70-71	0.4290769142690607
1104	72-73	0.41380381378358777
1104	74-75	0.4655930714147658
1104	76-77	0.41016031695725275
1104	78-79	0.3620370271416462
1104	80-81	0.47540466074150345
1104	82-83	0.36162223093937484
1104	84-85	0.3570981474560284
1104	86-87	0.37818844189996526
1104	88-89	0.3087235474196852
1104	90-91	0.4005248356612974
1104	92-93	0.3461101314942532
1104	94-95	0.34053338083775486
1104	96-97	0.3403825831089655
1104	98-99	0.4608054305271203
1104	100	0.45580975736363527
1105	1	0.501651171901603
1105	2	0.6725104360436447
1105	3	0.6007620461369996
1105	4	0.6920262096866452
1105	5	0.6379213750578003
1105	6	0.2713216565355836
1105	7	0.4183901854947294
1105	8	0.427198076624407
1105	9	0.5673640876623196
1105	10-11	0.5465657507986919
1105	12-13	0.5143960801367271
1105	14-15	0.6359462513752163
1105	16-17	0.7641239065206591
1105	18-19	0.7997957369673756
1105	20-21	0.6312707244357938
1105	22-23	0.8905339333095412
1105	24-25	0.7741089579402924
1105	26-27	0.6394225858647289
1105	28-29	0.8834228224058052
1105	30-31	0.8883347139823954
1105	32-33	0.7490569362615744
1105	34-35	0.6461926866146257
1105	36-37	0.5391973155225429
1105	38-39	0.7157339624624797
1105	40-41	0.5565662169445034
1105	42-43	0.5860020993692459
1105	44-45	0.6816071190650916
1105	46-47	0.5938527047675208
1105	48-49	0.6421307753751506
1105	50-51	0.7269172509921802
1105	52-53	0.7366590642614312
1105	54-55	0.7262737683003806
1105	56-57	0.6723782060751375
1105	58-59	0.5859177476194475
1105	60-61	0.5377898996802557
1105	62-63	0.6028843707520011
1105	64-65	0.5840355413848428
1105	66-67	0.6593311983494559
1105	68-69	0.6603709641423237
1105	70-71	0.5689496343888649
1105	72-73	0.5789669795124013
1105	74-75	0.574874590595833
1105	76-77	0.5288771096724716
1105	78-79	0.5674698429556599
1105	80-81	0.6395128786061619
1105	82-83	0.5602186581590445
1105	84-85	0.5455971014487275
1105	86-87	0.5693289694386152
1105	88-89	0.6352758682257189
1105	90-91	0.6905776055358679
1105	92-93	0.6936855966980424
1105	94-95	0.5870051772878746
1105	96-97	0.5880784834615689
1105	98-99	0.6824927940421617
1105	100	0.6842700288517349
1106	1	0.48515111578448966
1106	2	0.533882598381112
1106	3	0.49032217175167503
1106	4	0.7104116358514645
1106	5	0.5909477789274078
1106	6	0.3161315788562433
1106	7	0.32441491533910494
1106	8	0.36031663425908533
1106	9	0.5223249785581956
1106	10-11	0.4988129866408144
1106	12-13	0.43096260274153053
1106	14-15	0.5806342552996213
1106	16-17	0.7395141989275658
1106	18-19	0.7352433804726815
1106	20-21	0.5414779132586602
1106	22-23	0.8213347754607412
1106	24-25	0.657950951946269
1106	26-27	0.5236663077673427
1106	28-29	0.729253444529185
1106	30-31	0.7891755012576951
1106	32-33	0.6175499389227355
1106	34-35	0.5362609860293546
1106	36-37	0.48676108631317305
1106	38-39	0.6203979586265618
1106	40-41	0.5476312584210206
1106	42-43	0.5412160780665047
1106	44-45	0.6417002314491995
1106	46-47	0.5938644876857424
1106	48-49	0.5875090855597804
1106	50-51	0.588888885408501
1106	52-53	0.5606399017814567
1106	54-55	0.658750311591298
1106	56-57	0.527957426875652
1106	58-59	0.576744320363801
1106	60-61	0.5932766928982147
1106	62-63	0.5892629821100854
1106	64-65	0.5933358477226633
1106	66-67	0.6852724917255912
1106	68-69	0.5723136195412835
1106	70-71	0.5707774950293754
1106	72-73	0.5563641323623045
1106	74-75	0.5964892969566122
1106	76-77	0.5888383951800833
1106	78-79	0.6418347920071739
1106	80-81	0.5852327645031679
1106	82-83	0.605821426489932
1106	84-85	0.5640051394719166
1106	86-87	0.6381875829026598
1106	88-89	0.5827423977120532
1106	90-91	0.6162795509730223
1106	92-93	0.7155218580178051
1106	94-95	0.6382056181598124
1106	96-97	0.6496792576173469
1106	98-99	0.6838973591468971
1106	100	0.6248525108391441
1107	1	0.4610358560817822
1107	2	0.6179618329573522
1107	3	0.6027314296513744
1107	4	0.7532404512580939
1107	5	0.6558663261274376
1107	6	0.2801730542527494
1107	7	0.35359602057823025
1107	8	0.41527815037891713
1107	9	0.5827724704242314
1107	10-11	0.5483656759172035
1107	12-13	0.4356502840391627
1107	14-15	0.6240979448803543
1107	16-17	0.7524431029789795
1107	18-19	0.8584781990702979
1107	20-21	0.5837276609988677
1107	22-23	0.8083281343817816
1107	24-25	0.45743051117261757
1107	26-27	0.4600871559598616
1107	28-29	0.6237776812431903
1107	30-31	0.6538887772233295
1107	32-33	0.5623606747460457
1107	34-35	0.4821589946832532
1107	36-37	0.4733608198482102
1107	38-39	0.49674244783325605
1107	40-41	0.4128862888026319
1107	42-43	0.4704879950006955
1107	44-45	0.4653240122567084
1107	46-47	0.424388815049376
1107	48-49	0.4818832328278475
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2210	4	-0.01407517063723418
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2210	22-23	-1.6353717076678436
2210	24-25	-0.7844170414828326
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2214	3	-1.048232100087251
2214	4	-0.7651290447811121
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2214	98-99	-1.1627367940647204
2214	100	-1.0727221709774213
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2215	2	-2.4498284505084165
2215	3	-3.9175180055989465
2215	4	-2.1395155898951543
2215	5	-1.6125274396418945
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2215	7	-1.3509339687775466
2215	8	-1.2915335850555607
2215	9	-1.8862299838053644
2215	10-11	-1.4518443008065347
2215	12-13	-1.4601209057285587
2215	14-15	-3.7750953167673558
2215	16-17	-3.5285875580893347
2215	18-19	-2.375213751690815
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2215	22-23	-3.3298504822287995
2215	24-25	-5.646494286130405
2215	26-27	-3.7088978428545545
2215	28-29	-1.901170295644313
2215	30-31	-4.28700461642412
2215	32-33	-4.2955264958876285
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2215	36-37	-2.068423705134954
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2215	100	-3.1684449878777166
2216	1	-0.7264975878515223
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2216	3	-0.8837988429172476
2216	4	-0.8378828746277094
2216	5	-0.942521383018402
2216	6	-0.8198757693780436
2216	7	-0.9722584296192949
2216	8	-0.8410751817873603
2216	9	-1.013788709301771
2216	10-11	-1.0515413211852902
2216	12-13	-1.294555630765764
2216	14-15	-1.6000586504437635
2216	16-17	-1.3207617473726785
2216	18-19	-3.1357893387478555
2216	20-21	-1.5956145571787488
2216	22-23	-1.126600292217077
2216	24-25	-1.249840620091831
2216	26-27	-1.1905067868644394
2216	28-29	-1.0742702279526384
2216	30-31	-1.1960807933091928
2216	32-33	-1.223839379869645
2216	34-35	-1.3647883553164704
2216	36-37	-1.4442498107656334
2216	38-39	-1.499245365829104
2216	40-41	-1.5719048710352297
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2216	54-55	-1.3789886136963716
2216	56-57	-1.434555299883101
2216	58-59	-1.541552924578511
2216	60-61	-1.4631398098354822
2216	62-63	-1.485037814081629
2216	64-65	-1.5275363317849227
2216	66-67	-1.5143813579262755
2216	68-69	-1.6674687908702026
2216	70-71	-1.6856591513458952
2216	72-73	-1.6751675151541932
2216	74-75	-1.6565572819419678
2216	76-77	-1.522506082352173
2216	78-79	-1.5960002904060389
2216	80-81	-1.5496844194687576
2216	82-83	-1.6562629001336262
2216	84-85	-1.5591333841781534
2216	86-87	-1.5082659282726212
2216	88-89	-1.3933390160496515
2216	90-91	-1.3266593415644898
2216	92-93	-1.3434200058639796
2216	94-95	-1.2414938816679104
2216	96-97	-1.3161823256406358
2216	98-99	-1.2419841103206863
2216	100	-1.1962544892553026
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	16086.0
1	2735.0
2	425.0
3	390.0
4	367.0
5	219.0
6	191.0
7	225.0
8	462.0
9	772.0
10	1408.0
11	2647.0
12	4469.0
13	6497.0
14	8553.0
15	10817.0
16	12840.0
17	15073.0
18	17909.0
19	20446.0
20	22901.0
21	26197.0
22	30406.0
23	34887.0
24	39268.0
25	44118.0
26	49958.0
27	57272.0
28	68218.0
29	81261.0
30	97344.0
31	115348.0
32	141840.0
33	180230.0
34	245920.0
35	331896.0
36	485722.0
37	752734.0
38	719550.0
39	236425.0
40	388.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.16291259429389	19.997196668039727	12.759191184119098	24.08069955354728
2	15.473826337854463	19.870839170405972	40.58630285918071	24.06903163255885
3	18.80194794443433	25.898456256105433	29.058743604814808	26.24085219464543
4	12.282735665649602	16.684126447092467	39.26952934639894	31.76360854085899
5	13.324383008069516	38.08018626054393	35.167770761345665	13.427659970040889
6	30.697785722970895	37.633551251238565	18.176034894460297	13.492628131330248
7	27.22889253095048	31.815380252447657	23.13462650094884	17.821100715653028
8	25.810474073575723	35.17640939918594	20.401206855060952	18.611909672177383
9	25.973415954826635	15.597377568129994	21.04350697597622	37.385699501067144
10-11	25.209016855939232	25.927951497632755	28.10498286038487	20.758048786043144
12-13	25.613125987809237	23.76874953007683	27.863037244314885	22.75508723779905
14-15	22.90089642400972	25.181258306513083	25.253300929035365	26.664544340441832
16-17	22.352870987859706	27.844933382976933	26.453307905872325	23.34888772329104
18-19	21.980037418678048	28.017872550020662	27.805207012100308	22.196883019200982
20-21	23.35063705143919	27.196382253381252	26.993528479311053	22.45945221586851
22-23	23.490749866788924	26.9744313643104	26.790025061405164	22.74479370749551
24-25	23.10958206538935	27.08338698437574	27.262580387594543	22.54445056264037
26-27	22.923657072295857	27.29490806497982	26.63881156872401	23.14262329400032
28-29	22.427099885710557	27.6742441700158	26.97767765357425	22.920978290699388
30-31	22.959192717825573	27.353092529053093	26.86670157708934	22.82101317603199
32-33	23.124073930061286	27.55404444204056	26.270191737479625	23.051689890418533
34-35	23.30352582232715	27.429419559126295	26.840464657076446	22.426589961470107
36-37	23.139204926041934	27.03756000837195	26.661249318355118	23.161985747230997
38-39	23.027167769655456	27.21423263222988	27.060982462879444	22.69761713523522
40-41	22.9128304317624	26.692235022650802	27.443909483774533	22.95102506181227
42-43	22.820473096636178	26.829785050320243	26.98998875674909	23.35975309629449
44-45	22.958236265512838	27.37293122252514	26.9231725081669	22.745660003795127
46-47	22.979591403516856	27.22301639035311	26.579779035445554	23.21761317068448
48-49	23.267267093179033	26.987541717073515	26.75138597540279	22.993805214344658
50-51	23.01039309465216	26.994660766828034	26.762597283597987	23.23234885492182
52-53	22.807321769148885	27.306636029454584	26.61194121402972	23.274100987366808
54-55	22.637625038665078	27.21621772031978	26.72822880927082	23.417928431744322
56-57	23.303388260743027	26.954589870351608	26.532297961344497	23.209723907560868
58-59	22.714302519596814	27.06601102165883	27.05020959217947	23.169476866564885
60-61	22.627607820361813	27.4799284954673	26.756290615664174	23.136173068506714
62-63	22.874213049509898	27.050444928767014	26.53996957333965	23.535372448383438
64-65	22.33712179692829	27.18709975151513	26.73942321641478	23.736355235141797
66-67	22.764735395632822	26.960544840267573	26.6664199233655	23.6082998407341
68-69	22.529966995725676	26.948550455067565	26.885539706220797	23.635942842985962
70-71	22.498253834995076	26.646820931409653	26.995109184976755	23.859816048618516
72-73	22.777495494175625	26.754463050428217	27.248971289816332	23.219070165579826
74-75	22.874201822704343	27.00639836003199	26.956974134784872	23.162425682478798
76-77	22.72106207987623	26.84937274931673	26.743820564650488	23.68574460615655
78-79	22.59258741501327	26.828610992673983	26.841567884925194	23.73723370738755
80-81	22.371343287908562	27.064036994102363	27.20054941428679	23.364070303702285
82-83	22.830827754596665	26.44076600674262	27.831775013988896	22.89663122467181
84-85	22.947302458473153	26.82518624296594	26.966951296831876	23.26056000172903
86-87	22.153644510434372	27.172897751341416	27.28781612126095	23.38564161696326
88-89	22.741652940780586	26.382873530794303	27.19527068428006	23.680202844145054
90-91	22.51568782369763	26.427289328895665	27.315009937899514	23.742012909507192
92-93	22.673461989773173	26.544101807494336	27.346706609039618	23.435729593692873
94-95	21.917145785195313	26.375203274732677	27.837753017083212	23.869897922988798
96-97	22.19648103727647	26.26564043812165	27.522667579864812	24.015210944737063
98-99	21.74892077117225	26.77084895169553	27.386680973086435	24.09354930404578
100	22.260190891475894	26.238441507599113	27.056555269922878	24.444812331002115
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13598.0
1	8018.5
2	2314.0
3	1751.0
4	1007.5
5	643.0
6	546.5
7	499.5
8	465.5
9	451.5
10	435.0
11	408.5
12	405.0
13	421.5
14	439.5
15	534.0
16	645.5
17	720.0
18	928.0
19	1262.0
20	1638.0
21	2171.0
22	2985.0
23	4102.5
24	5613.5
25	7605.0
26	10594.0
27	14278.5
28	19083.0
29	26823.5
30	36633.0
31	46236.0
32	58208.5
33	68709.0
34	76525.5
35	86460.0
36	96944.5
37	111774.0
38	119595.5
39	116727.0
40	113623.0
41	111080.5
42	112440.0
43	118179.0
44	126713.0
45	142599.0
46	157276.5
47	166308.0
48	172704.5
49	169610.0
50	161446.0
51	161368.5
52	160320.5
53	153545.5
54	146238.0
55	130397.5
56	116345.0
57	100453.0
58	84218.0
59	77225.5
60	64449.5
61	48594.0
62	38131.0
63	30687.0
64	22844.0
65	14635.0
66	10064.0
67	7504.0
68	5594.5
69	4274.5
70	3329.0
71	2624.5
72	2027.0
73	1628.0
74	1319.5
75	1012.0
76	725.5
77	462.0
78	289.5
79	202.0
80	136.0
81	96.0
82	67.5
83	41.0
84	30.5
85	26.0
86	20.0
87	21.5
88	16.5
89	8.5
90	10.0
91	8.0
92	7.5
93	9.5
94	7.0
95	4.0
96	3.0
97	2.0
98	1.0
99	1.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.003755006546676
2	0.4532730033410445
3	0.48928873183960314
4	0.47332750834488807
5	0.4413278296288707
6	0.490267000376376
7	0.5069490533192395
8	0.5103472492890819
9	0.5243261918013888
10-11	0.5367604997819491
12-13	0.534198980850136
14-15	0.5336454868095935
16-17	0.5373912255490789
18-19	0.5363872131034436
20-21	0.535305968931221
22-23	0.49705052036162983
24-25	0.5331949684045006
26-27	0.5120720911828657
28-29	0.5175169279072726
30-31	0.5210180995125648
32-33	0.5226657096797612
34-35	0.5352287372046337
36-37	0.5538930711299053
38-39	0.5437885869013962
40-41	0.5484868502687921
42-43	0.5459639472002726
44-45	0.5551673946185963
46-47	0.5543178456261356
48-49	0.5261668812850536
50-51	0.5414330192404826
52-53	0.5329761451791699
54-55	0.543428172177322
56-57	0.5307750409714309
58-59	0.5364901887388934
60-61	0.5304017542929256
62-63	0.5243648076646825
64-65	0.5354346884755332
66-67	0.5341732369412735
68-69	0.5555792971603954
70-71	0.5392705308960373
72-73	0.5427073427291736
74-75	0.5386655490377699
76-77	0.526424320373678
78-79	0.5561971509730941
80-81	0.5319463888246722
82-83	0.5312513032853862
84-85	0.5400943359796355
86-87	0.8491499618732711
88-89	1.1181995533946691
90-91	1.108506971707959
92-93	1.1081851728471785
94-95	1.1133082107108048
96-97	1.1062028918647704
98-99	1.1013759089530621
100	1.0979777129832198
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3884414.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.530179515878814
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.51215492801484	34.07099994091233
2	12.763011692051245	11.366784036206043
3	4.546458866904152	6.073638885144026
4	1.9985161057371132	3.5597712381539486
5	1.1455860827464552	2.5506576957796034
6	0.6671840673636817	1.782589577390336
7	0.41626691504904423	1.297550831755851
8	0.3090902012038522	1.1011073716965314
9	0.21506112668399055	0.8619039520312729
>10	1.1591005614929866	9.525845671880282
>50	0.11596028665744051	3.6135526860222025
>100	0.12493805290137704	11.782267171474063
>500	0.019108524826793323	5.803821875172717
>1k	0.0071584805917119295	5.317674313655304
>5k	3.4637809314735143E-4	0.9798787166608617
>10k+	5.772968219122523E-5	0.3119560360647364
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	12135	0.3124023340457531	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	8170	0.21032773540616423	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	6966	0.17933206913578212	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	6642	0.1709910426643504	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	5767	0.14846512240971224	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	5436	0.1399438885762434	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	5136	0.13222071591751033	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	4447	0.11448316271128668	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.28719544312218E-4
2	0.0	0.0	0.0	0.0	1.28719544312218E-4
3	0.0	0.0	0.0	0.0	1.28719544312218E-4
4	0.0	0.0	0.0	0.0	1.28719544312218E-4
5	0.0	0.0	0.0	0.0	1.5446345317466162E-4
6	0.0	0.0	0.0	0.0	1.5446345317466162E-4
7	0.0	0.0	0.0	0.0	1.5446345317466162E-4
8	2.57439088624436E-5	0.0	0.0	0.0	1.5446345317466162E-4
9	2.57439088624436E-5	0.0	0.0	0.0	1.5446345317466162E-4
10-11	2.57439088624436E-5	0.0	0.0	2.57439088624436E-5	1.802073620371052E-4
12-13	2.57439088624436E-5	0.0	0.0	3.8615863293665404E-5	5.020062228176502E-4
14-15	2.57439088624436E-5	0.0	0.0	5.14878177248872E-5	5.792379494049811E-4
16-17	2.57439088624436E-5	0.0	0.0	5.14878177248872E-5	6.693416304235336E-4
18-19	2.57439088624436E-5	0.0	0.0	5.14878177248872E-5	6.822135848547554E-4
20-21	2.57439088624436E-5	0.0	0.0	5.14878177248872E-5	8.36677038029417E-4
22-23	2.57439088624436E-5	0.0	0.0	6.4359772156109E-5	9.525246279104133E-4
24-25	2.57439088624436E-5	0.0	0.0	3.21798860780545E-4	0.001029756354497744
26-27	2.57439088624436E-5	0.0	0.0	6.564696759923118E-4	0.0010683722177914094
28-29	2.57439088624436E-5	0.0	0.0	0.0018535614380959394	0.0010812441722226312
30-31	2.57439088624436E-5	0.0	0.0	0.006165666172555243	0.0010812441722226312
32-33	2.57439088624436E-5	0.0	0.0	0.016102814993458472	0.0011456039443787402
34-35	2.57439088624436E-5	0.0	0.0	0.03268189230087215	0.0012614515342597365
36-37	2.57439088624436E-5	0.0	0.0	0.06102593595842255	0.0012743234886909583
38-39	2.57439088624436E-5	0.0	0.0	0.10903832598687987	0.0013386832608470673
40-41	2.57439088624436E-5	0.0	0.0	0.1647867606285015	0.0013644271697095109
42-43	3.8615863293665404E-5	0.0	0.0	0.2220025980752824	0.0013772991241407326
44-45	5.14878177248872E-5	0.0	0.0	0.2922963412241847	0.001480274759590507
46-47	5.14878177248872E-5	0.0	0.0	0.38364860182256577	0.0015961223494715033
48-49	5.14878177248872E-5	0.0	0.0	0.48927585988517186	0.0016862260304900559
50-51	5.14878177248872E-5	0.0	0.0	0.6024332112900428	0.0017505858026461648
52-53	5.14878177248872E-5	0.0	0.0	0.7142132635707729	0.001802073620371052
54-55	5.14878177248872E-5	0.0	0.0	0.8439883081463511	0.001879305346958383
56-57	5.14878177248872E-5	0.0	0.0	1.0062264217974706	0.0019179212102520484
58-59	5.14878177248872E-5	0.0	0.0	1.1979541830505194	0.002008024891270601
60-61	5.14878177248872E-5	0.0	0.0	1.4048965944412721	0.002008024891270601
62-63	5.14878177248872E-5	0.0	0.0	1.6187383734071599	0.0021624883444452624
64-65	5.14878177248872E-5	0.0	0.0	1.8384755075025474	0.002188232253307706
66-67	5.14878177248872E-5	0.0	0.0	2.0943570896408055	0.0022268481166013714
68-69	5.14878177248872E-5	0.0	0.0	2.3871425651333764	0.0022654639798950368
70-71	5.14878177248872E-5	0.0	0.0	2.714077335731979	0.0022783359343262585
72-73	5.14878177248872E-5	0.0	0.0	3.0614785138762244	0.0023684396153448115
74-75	5.14878177248872E-5	0.0	0.0	3.4009119522275433	0.002458543296363364
76-77	5.14878177248872E-5	0.0	0.0	3.78309315124495	0.0025100311140882512
78-79	5.14878177248872E-5	0.0	0.0	4.210236087090614	0.002522903068519473
80-81	5.14878177248872E-5	0.0	0.0	4.684915150650781	0.0025615189318131384
82-83	5.14878177248872E-5	0.0	0.0	5.173714748221997	0.0026258787039692474
84-85	5.14878177248872E-5	0.0	0.0	5.65520307567628	0.0026258787039692474
86-87	5.14878177248872E-5	0.0	0.0	6.157994487714234	0.0027545982482814653
88	5.14878177248872E-5	0.0	0.0	6.560757941867164	0.0028833177925936832
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	2810	0.0	32.570084	1
TAGGCAT	3585	0.0	27.52074	5
GCATCGT	2980	0.0	26.939476	8
GGGCACC	3015	0.0	26.153599	94
GGTATCA	6020	0.0	25.782373	1
GTATCAA	8525	0.0	25.345135	1
CTAACGC	1360	0.0	25.223747	3
GTACATG	14785	0.0	25.143652	1
CTGTGCG	3590	0.0	24.848938	9
TACATGG	14735	0.0	24.74848	2
ACATGGG	14475	0.0	24.445677	3
CAACGCA	7905	0.0	24.308123	5
TCTAACG	1400	0.0	23.832373	2
TAACGCC	1450	0.0	23.654764	4
CTAGGCA	4200	0.0	23.604616	4
TAGTACT	3810	0.0	23.554405	4
ATCAACG	8285	0.0	23.425194	3
CATGGGG	11220	0.0	23.157734	4
GCTATTG	3370	0.0	23.125305	8
CGCCTAC	1525	0.0	22.471903	7
>>END_MODULE