##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146481_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3884414
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.85009682284123	31.0	31.0	34.0	30.0	34.0
2	31.80749605989475	31.0	31.0	34.0	30.0	34.0
3	31.983696897395593	33.0	31.0	34.0	30.0	34.0
4	35.725655143864685	37.0	35.0	37.0	35.0	37.0
5	35.64122670755486	37.0	35.0	37.0	33.0	37.0
6	35.68439666832629	37.0	35.0	37.0	33.0	37.0
7	35.60472390430062	37.0	35.0	37.0	33.0	37.0
8	35.58863653565248	37.0	35.0	37.0	33.0	37.0
9	37.179188418124326	39.0	37.0	39.0	33.0	39.0
10-11	37.129824987758774	39.0	37.0	39.0	33.0	39.0
12-13	37.07718113980641	39.0	37.0	39.0	33.0	39.0
14-15	38.28901939906508	40.0	38.0	41.0	33.0	41.0
16-17	38.28783221870789	40.0	38.0	41.0	33.0	41.0
18-19	38.3460816740955	40.0	38.0	41.0	33.0	41.0
20-21	38.36282538370009	40.0	38.0	41.0	33.0	41.0
22-23	38.33372150857247	40.0	38.0	41.0	33.0	41.0
24-25	38.27662641005825	40.0	38.0	41.0	33.0	41.0
26-27	38.14512896926023	40.0	37.0	41.0	33.0	41.0
28-29	38.07348997815372	40.0	37.0	41.0	33.0	41.0
30-31	37.9428613942798	40.0	37.0	41.0	33.0	41.0
32-33	37.819950190685134	40.0	37.0	41.0	33.0	41.0
34-35	37.72460582213945	40.0	36.5	41.0	32.5	41.0
36-37	37.635828724744584	40.0	36.0	41.0	32.0	41.0
38-39	37.52941241587534	40.0	36.0	41.0	32.0	41.0
40-41	37.41364823625906	40.0	36.0	41.0	31.0	41.0
42-43	37.29583780204685	40.0	36.0	41.0	31.0	41.0
44-45	37.12602338473705	39.0	35.5	41.0	31.0	41.0
46-47	36.90905024541668	39.0	35.0	41.0	30.5	41.0
48-49	36.677476062026344	39.0	35.0	41.0	30.0	41.0
50-51	36.50269912012469	39.0	35.0	41.0	30.0	41.0
52-53	36.33384379213956	39.0	35.0	41.0	29.5	41.0
54-55	36.15267566742371	38.5	35.0	40.5	29.0	41.0
56-57	35.934034451528596	38.0	35.0	40.0	29.0	41.0
58-59	35.69488679630956	38.0	34.0	40.0	28.0	41.0
60-61	35.44752117050345	37.0	34.0	40.0	28.0	41.0
62-63	35.189662584884104	37.0	34.0	40.0	28.0	41.0
64-65	34.927591394738045	36.0	34.0	39.5	28.0	41.0
66-67	34.70069063184305	36.0	34.0	39.0	28.0	41.0
68-69	34.50141823193923	35.5	33.5	39.0	28.0	41.0
70-71	34.246440775880224	35.0	33.0	39.0	27.0	41.0
72-73	34.0152669102727	35.0	33.0	38.0	27.0	40.0
74-75	33.81393358174489	35.0	33.0	37.0	27.0	39.5
76-77	33.616658523010166	35.0	33.0	37.0	27.0	39.0
78-79	33.46359785542941	35.0	33.0	36.0	27.0	39.0
80-81	33.27512669864747	35.0	33.0	36.0	27.0	38.5
82-83	33.069283037286965	35.0	33.0	36.0	27.0	37.0
84-85	32.902298004280695	35.0	33.0	35.0	27.0	37.0
86-87	32.74730371170529	35.0	33.0	35.0	27.0	36.5
88-89	32.617101189523055	35.0	33.0	35.0	27.0	36.0
90-91	32.517152522877325	35.0	33.0	35.0	26.0	36.0
92-93	32.43481243245442	35.0	33.0	35.0	26.0	36.0
94-95	32.375041125894406	35.0	33.0	35.0	26.0	35.5
96-97	32.3202609196651	35.0	33.0	35.0	26.0	35.0
98-99	32.27259066618543	35.0	33.0	35.0	26.0	35.0
100	31.78616954835401	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4597646741771797
1101	2	0.5906319739654258
1101	3	0.44278628104801143
1101	4	0.30377744501776505
1101	5	0.32029268374428455
1101	6	0.37339736072005536
1101	7	0.4072590047170692
1101	8	0.4139466002174714
1101	9	0.435766250025182
1101	10-11	0.500479256688152
1101	12-13	0.5212357093218287
1101	14-15	0.6045730332213921
1101	16-17	0.6286309271782073
1101	18-19	0.6471075916653533
1101	20-21	0.6356003345723025
1101	22-23	0.6465489343550246
1101	24-25	0.6868607872131562
1101	26-27	0.7094486274698539
1101	28-29	0.7144511142705738
1101	30-31	0.7924399789813776
1101	32-33	0.773012188270954
1101	34-35	0.7458556000644307
1101	36-37	0.7835235733006414
1101	38-39	0.817747601470586
1101	40-41	0.8470862731281841
1101	42-43	0.8320794780020933
1101	44-45	0.8408530285027211
1101	46-47	0.8208434215154554
1101	48-49	0.8788217964000751
1101	50-51	0.7708191079025255
1101	52-53	0.850537315116469
1101	54-55	0.7519792134866705
1101	56-57	0.7960907705020119
1101	58-59	0.7965502729624205
1101	60-61	0.7731339358018303
1101	62-63	0.7723059930454141
1101	64-65	0.8412117847513585
1101	66-67	0.8818532878664627
1101	68-69	0.8152752723066712
1101	70-71	0.80131270642093
1101	72-73	0.7933629700924172
1101	74-75	0.7211135638591628
1101	76-77	0.671560679313778
1101	78-79	0.7260318561135719
1101	80-81	0.652695827401395
1101	82-83	0.569041605500459
1101	84-85	0.6194101901353477
1101	86-87	0.5829500685901223
1101	88-89	0.5820641078635376
1101	90-91	0.6258270943497308
1101	92-93	0.6069687407865416
1101	94-95	0.6038707450231797
1101	96-97	0.6275842667677551
1101	98-99	0.6225265248150365
1101	100	0.650894310266068
1102	1	0.44425417748959006
1102	2	0.5491296206921312
1102	3	0.5034023199249198
1102	4	0.3429489653663822
1102	5	0.31950556471750247
1102	6	0.3291537125527526
1102	7	0.4141280420739122
1102	8	0.3637654394848795
1102	9	0.46062926401862825
1102	10-11	0.4921074099016778
1102	12-13	0.512467534063731
1102	14-15	0.628901441431033
1102	16-17	0.6498685349746651
1102	18-19	0.6159656696167559
1102	20-21	0.6569210767307894
1102	22-23	0.6112418823459578
1102	24-25	0.6029567860239027
1102	26-27	0.670639407589583
1102	28-29	0.6471799302178383
1102	30-31	0.7507345075304102
1102	32-33	0.7071793008580087
1102	34-35	0.7650149669173345
1102	36-37	0.753747049489121
1102	38-39	0.7291650188530099
1102	40-41	0.7159240287633963
1102	42-43	0.7578662794516688
1102	44-45	0.6841978649546618
1102	46-47	0.7528604577212477
1102	48-49	0.7516328496193978
1102	50-51	0.7280648705565653
1102	52-53	0.6908083717183828
1102	54-55	0.7350513776298797
1102	56-57	0.7686154871942819
1102	58-59	0.7439654990096756
1102	60-61	0.6791884716283718
1102	62-63	0.5970913817226418
1102	64-65	0.674454123030813
1102	66-67	0.7256084550574755
1102	68-69	0.6373496540311052
1102	70-71	0.6673045635315589
1102	72-73	0.6369659495649671
1102	74-75	0.672183368587504
1102	76-77	0.5895591886431433
1102	78-79	0.545395598329911
1102	80-81	0.6006118674436252
1102	82-83	0.6186928978611945
1102	84-85	0.5543848018066768
1102	86-87	0.5808205428656876
1102	88-89	0.48942939765193927
1102	90-91	0.5546408920659687
1102	92-93	0.5759042314611591
1102	94-95	0.5383110658632901
1102	96-97	0.5845853375015935
1102	98-99	0.55703113535359
1102	100	0.6138334972243484
1103	1	0.22463500818795978
1103	2	-0.201734236180517
1103	3	0.15625622387799254
1103	4	0.19536767513731235
1103	5	0.17973343325020608
1103	6	0.24810953282757708
1103	7	0.2547918563576559
1103	8	0.2881790815340892
1103	9	0.3449180740524298
1103	10-11	0.3923536381889363
1103	12-13	0.371652153385142
1103	14-15	0.488397425011037
1103	16-17	0.4746160883494781
1103	18-19	0.5009908382276578
1103	20-21	0.4779170486793731
1103	22-23	0.4334743640801477
1103	24-25	0.43728326397519623
1103	26-27	0.4708893937213503
1103	28-29	0.45867481865459325
1103	30-31	0.5394300165483443
1103	32-33	0.5311403910623724
1103	34-35	0.4746812166422103
1103	36-37	0.4928712211975679
1103	38-39	0.5501260965312298
1103	40-41	0.5941403410295365
1103	42-43	0.5724390214792194
1103	44-45	0.569494135204522
1103	46-47	0.5733482259374014
1103	48-49	0.5817514364551357
1103	50-51	0.6487152997560841
1103	52-53	0.6124259985143254
1103	54-55	0.5698789015145636
1103	56-57	0.5610724196121311
1103	58-59	0.5383416162973731
1103	60-61	0.5313996052269019
1103	62-63	0.5097384968612104
1103	64-65	0.4520720432965035
1103	66-67	0.4723610511017142
1103	68-69	0.47531587279849674
1103	70-71	0.4824884026528693
1103	72-73	0.4600367885126957
1103	74-75	0.45894090351308847
1103	76-77	0.42325041192889756
1103	78-79	0.4363035830336983
1103	80-81	0.4368834772863224
1103	82-83	0.4208665424872038
1103	84-85	0.3674991422781204
1103	86-87	0.4078970651058711
1103	88-89	0.4331896758040088
1103	90-91	0.42943488166211097
1103	92-93	0.46620022434630926
1103	94-95	0.4417111993562415
1103	96-97	0.4261483502907879
1103	98-99	0.4436793348987109
1103	100	0.4537457426080138
1104	1	0.3244252391142055
1104	2	0.39724690262940143
1104	3	0.294683983338782
1104	4	0.24426472004327593
1104	5	0.2523714581160448
1104	6	0.25264201098089245
1104	7	0.29564665181327854
1104	8	0.2670663740709145
1104	9	0.3404312631853017
1104	10-11	0.3318267578834906
1104	12-13	0.3025767777266424
1104	14-15	0.4218938386268647
1104	16-17	0.35278177767612817
1104	18-19	0.4465148733409876
1104	20-21	0.3899943033063451
1104	22-23	0.3642676949738899
1104	24-25	0.3963200087148877
1104	26-27	0.5112905207419018
1104	28-29	0.47542690117298037
1104	30-31	0.543470970489885
1104	32-33	0.48259216009887496
1104	34-35	0.45462726915597784
1104	36-37	0.5503383933289854
1104	38-39	0.5090207318869631
1104	40-41	0.5795063793503985
1104	42-43	0.5384048755686379
1104	44-45	0.5158254597781493
1104	46-47	0.4875583230945537
1104	48-49	0.5211530507936999
1104	50-51	0.5473982191695512
1104	52-53	0.5266099571589891
1104	54-55	0.48392435608732853
1104	56-57	0.5250986158096111
1104	58-59	0.46647813264054605
1104	60-61	0.5624404407940276
1104	62-63	0.4378083149311536
1104	64-65	0.39563448820283753
1104	66-67	0.45331088258342334
1104	68-69	0.489504878880048
1104	70-71	0.4800509112530378
1104	72-73	0.5258358368304812
1104	74-75	0.5003280833839128
1104	76-77	0.48007193275768856
1104	78-79	0.4263553252707055
1104	80-81	0.4181980461721153
1104	82-83	0.4182355660064587
1104	84-85	0.3911319628759884
1104	86-87	0.35581262389018065
1104	88-89	0.36841393522784216
1104	90-91	0.39731215203227066
1104	92-93	0.3694123170436896
1104	94-95	0.3848419081425689
1104	96-97	0.37465638202473883
1104	98-99	0.38434310890333023
1104	100	0.40729309928333635
1105	1	0.09016399539667219
1105	2	-0.49629473100286603
1105	3	-0.030077235311374295
1105	4	0.07301297653534533
1105	5	0.1293810746049857
1105	6	0.22162679587800937
1105	7	0.20761011483671865
1105	8	0.25323895626947746
1105	9	0.31943028827370057
1105	10-11	0.33585505488048284
1105	12-13	0.3466880774877552
1105	14-15	0.3620362389802878
1105	16-17	0.37650547996784667
1105	18-19	0.4017198931364163
1105	20-21	0.3912586007731278
1105	22-23	0.3959235900338811
1105	24-25	0.4433504164392943
1105	26-27	0.3416911446686939
1105	28-29	0.4326908762595636
1105	30-31	0.407688689502379
1105	32-33	0.44037127035604584
1105	34-35	0.43371057987583583
1105	36-37	0.4256291859047536
1105	38-39	0.4239054730402785
1105	40-41	0.4526003395844711
1105	42-43	0.46929997519995226
1105	44-45	0.42001333289248777
1105	46-47	0.4027608312145503
1105	48-49	0.4283859535131427
1105	50-51	0.4677901283830863
1105	52-53	0.3524183136726009
1105	54-55	0.3951908317776045
1105	56-57	0.4488283711767309
1105	58-59	0.4342705864333496
1105	60-61	0.3784567068033269
1105	62-63	0.33680287545409726
1105	64-65	0.35532039518523106
1105	66-67	0.40765135739351877
1105	68-69	0.4072566569937024
1105	70-71	0.3248908489923892
1105	72-73	0.31039496644969944
1105	74-75	0.27026321411592136
1105	76-77	0.286447422152996
1105	78-79	0.2031877002264224
1105	80-81	0.2662052055906585
1105	82-83	0.26921953123035536
1105	84-85	0.29248049139518884
1105	86-87	0.3720281085927297
1105	88-89	0.35713633321045535
1105	90-91	0.2992975529708062
1105	92-93	0.318331650756285
1105	94-95	0.3268439983118725
1105	96-97	0.28613401362397184
1105	98-99	0.26161197583128626
1105	100	0.2554395522084363
1106	1	0.2877780240720682
1106	2	0.3413596545541715
1106	3	0.26276597744024954
1106	4	0.16146682793686296
1106	5	0.15640080150478752
1106	6	0.16505870720219207
1106	7	0.21708141529626346
1106	8	0.2086225110129547
1106	9	0.22594760597925756
1106	10-11	0.25703941142447206
1106	12-13	0.2661956628896007
1106	14-15	0.34287120647373825
1106	16-17	0.3265805980052292
1106	18-19	0.3496422823285812
1106	20-21	0.3615173936211562
1106	22-23	0.3684042751365766
1106	24-25	0.2996015214101604
1106	26-27	0.308671939981636
1106	28-29	0.2916385678118516
1106	30-31	0.3653692171522849
1106	32-33	0.36561374644065836
1106	34-35	0.36752964102270624
1106	36-37	0.3232215618564709
1106	38-39	0.2832019209236023
1106	40-41	0.3247171310447854
1106	42-43	0.36893760276583265
1106	44-45	0.35447051512129946
1106	46-47	0.3239097100879249
1106	48-49	0.2846353696850059
1106	50-51	0.29000004743959096
1106	52-53	0.30508428142572086
1106	54-55	0.21428830716748593
1106	56-57	0.27032597801963476
1106	58-59	0.21934528510713847
1106	60-61	0.21230669230930488
1106	62-63	0.28695237799707485
1106	64-65	0.25660309346009313
1106	66-67	0.19813239154905204
1106	68-69	0.18884148476159623
1106	70-71	0.28689783474168706
1106	72-73	0.24240123723111395
1106	74-75	0.2541599097395988
1106	76-77	0.2560738564622298
1106	78-79	0.25166618385714656
1106	80-81	0.23231972436582993
1106	82-83	0.2585554745789125
1106	84-85	0.2680850550096565
1106	86-87	0.31871540559360767
1106	88-89	0.2860377663665403
1106	90-91	0.2115256540756718
1106	92-93	0.2490638838366479
1106	94-95	0.2866564735551762
1106	96-97	0.2798567348469021
1106	98-99	0.2198807083056451
1106	100	0.2955884924200234
1107	1	0.12451931401583494
1107	2	-0.4343835803051981
1107	3	-0.03164257382098157
1107	4	0.09249450647475754
1107	5	0.04850758395065924
1107	6	0.10701931188543767
1107	7	0.20129964859187766
1107	8	0.18783235202923265
1107	9	0.21001023452536316
1107	10-11	0.20988227203521603
1107	12-13	0.18860366690725527
1107	14-15	0.28004750749136775
1107	16-17	0.300978754879651
1107	18-19	0.24291057202275113
1107	20-21	0.24396553336545423
1107	22-23	0.23541047974853768
1107	24-25	0.22495605675538144
1107	26-27	0.2952864681224554
1107	28-29	0.25313378961161703
1107	30-31	0.2965762998894448
1107	32-33	0.3312026173631324
1107	34-35	0.3525932840971393
1107	36-37	0.28517303779744907
1107	38-39	0.3808881020847963
1107	40-41	0.3358968604901378
1107	42-43	0.3151986151084216
1107	44-45	0.27286671084787173
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2216	72-73	-0.8902365806946335
2216	74-75	-0.9700155782989413
2216	76-77	-0.8522893839798797
2216	78-79	-0.817547844097227
2216	80-81	-0.7266036027745528
2216	82-83	-0.6866702172845365
2216	84-85	-0.6542906938932802
2216	86-87	-0.6204804206635401
2216	88-89	-0.5938305263009198
2216	90-91	-0.6349481647105861
2216	92-93	-0.6625381459078454
2216	94-95	-0.624942686724701
2216	96-97	-0.6149139856390384
2216	98-99	-0.6727576447069339
2216	100	-0.7559432387208673
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	10.0
13	56.0
14	226.0
15	678.0
16	1634.0
17	3203.0
18	5816.0
19	8931.0
20	12912.0
21	18335.0
22	25070.0
23	30255.0
24	32520.0
25	33820.0
26	38016.0
27	46173.0
28	58042.0
29	72629.0
30	89732.0
31	111852.0
32	138399.0
33	174838.0
34	235301.0
35	322168.0
36	477219.0
37	812797.0
38	831918.0
39	301021.0
40	839.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.72857554816318	20.550609184629803	13.91325169675685	24.80756357045017
2	14.897549994387571	20.51113784023422	39.68826816908139	24.903043996296827
3	18.260738828408126	25.771566281933683	29.949754642939247	26.01794024671894
4	12.47641816750041	17.232364548992788	38.04427487442336	32.24694240908344
5	12.807521730321325	38.25561185886123	35.10033547708105	13.836530933736391
6	30.3791511409443	38.32699089231993	17.544396658028727	13.749461308707053
7	26.440041345026305	32.42917475996844	23.088056410474245	18.042727484531014
8	25.832236213750647	35.30522235786402	19.848322037764255	19.014219390621083
9	25.817047307521808	15.133814263876097	20.960819315345894	38.088319113256205
10-11	25.140574614343375	26.100745183185932	27.778488595705813	20.98019160676488
12-13	25.84653695512373	23.79568707146046	28.07702783482914	22.28074813858667
14-15	22.914220230372877	24.970707130261676	25.945835932638374	26.169236706727073
16-17	21.86263486242311	27.68906484016031	27.01421691136008	23.434083386056496
18-19	21.501814688135713	27.0765036888447	29.49728067090686	21.924400952112723
20-21	22.64557964571982	26.276284065166628	29.1007530999226	21.97738318919095
22-23	22.77196332803348	26.045455341181512	28.882456008468782	22.300125322316227
24-25	22.42579220775543	26.19118690633734	29.340207987640866	22.042812898266366
26-27	22.278049361923856	26.375890707754664	28.75957255810469	22.586487372216794
28-29	21.83301007565105	26.768889721847362	29.019538082192064	22.378562120309525
30-31	22.42110392970471	26.39494657366594	28.88632622578335	22.297623270846
32-33	22.621932162740634	26.60199196069214	28.39276400507258	22.383311871494644
34-35	22.689151131558138	26.531738938865857	28.827717430270127	21.95139249930588
36-37	22.570418601106883	26.166173327559832	28.638257919984845	22.625150151348443
38-39	22.585066885249617	26.24171882811667	29.01895884424266	22.15425544239105
40-41	22.36355966990522	25.84691662121777	29.29492705371834	22.494596655158666
42-43	22.464338310396844	25.942172835317194	28.64831515821486	22.9451736960711
44-45	22.59060182565504	26.51603304900044	28.443608225075906	22.44975690026861
46-47	22.74238791230801	26.45239410629248	27.891246916523315	22.913971064876197
48-49	23.207187943240047	26.398664295983405	27.702857601836683	22.69129015893987
50-51	22.848439944866843	26.537040078632195	27.51245104152132	23.102068934979638
52-53	22.583882665441944	26.794903941752864	27.402576038496413	23.218637354308783
54-55	22.47636700648749	26.758971784574193	27.399611265575118	23.365049943363196
56-57	23.13801838631864	26.47510175290456	27.198403361128204	23.188476499648594
58-59	22.461987119269427	26.63271382221276	27.736820575186893	23.16847848333092
60-61	22.500682857709958	26.9776421702029	27.41691082637754	23.10476414570961
62-63	22.74092050950285	26.56207603000092	27.16910452902291	23.527898931473317
64-65	22.125589599872722	26.64490705676583	27.45240337409967	23.777099969261773
66-67	22.558743738437766	26.444670933633745	27.356391465997188	23.640193861931298
68-69	22.37867861199875	26.32285220547773	27.59605984250955	23.702409340013965
70-71	22.331059742170154	26.122575178274747	27.639662767592334	23.90670231196276
72-73	22.658269690099974	26.206604136428304	27.76738009903167	23.367746074440056
74-75	22.70826666721587	26.542404027178407	27.4260009314163	23.32332837418942
76-77	22.58518037284181	26.479690106445137	27.096004262284996	23.83912525842806
78-79	22.446078132918306	26.557067403186625	27.172556024511934	23.82429843938314
80-81	22.222729965979596	26.884435532754857	27.450073520580936	23.442760980684614
82-83	22.718647183305272	26.24039090295906	28.045549565691985	22.995412348043683
84-85	22.824094205557046	26.587198299155006	27.126075546826144	23.4626319484618
86-87	22.00969853942622	26.96075528630902	27.418687517539503	23.610858656725256
88-89	22.52270564323398	26.263767144686756	27.25620775819644	23.957319453882825
90-91	22.2636710568762	26.289791932542656	27.38908642843663	24.05745058214451
92-93	22.509032755299643	26.519824559800014	27.2019332355576	23.769209449342743
94-95	21.738996676148307	26.374033332226325	27.668330008451868	24.2186399831735
96-97	21.966049942910253	26.290736786008058	27.370885129246815	24.372328141834878
98-99	21.57623316105595	26.763399617927025	27.20406689791165	24.456300323105374
100	22.03650506009841	26.28821419764904	26.785502174539833	24.889778567712714
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	206.0
1	157.5
2	187.0
3	255.0
4	175.5
5	149.0
6	328.5
7	856.5
8	1465.0
9	1689.0
10	1363.0
11	962.0
12	910.0
13	987.5
14	1174.5
15	1497.5
16	1855.5
17	2265.5
18	2834.0
19	3574.0
20	4394.0
21	5381.5
22	6530.5
23	7772.0
24	9251.5
25	11142.5
26	13799.5
27	17087.0
28	21865.5
29	29315.0
30	38487.5
31	47208.5
32	58791.5
33	69231.5
34	76039.0
35	84464.5
36	94141.5
37	109156.0
38	117510.0
39	115045.0
40	111899.5
41	109354.5
42	111020.0
43	116742.5
44	124612.5
45	140791.0
46	156045.0
47	166227.0
48	173983.0
49	170230.0
50	161140.0
51	160183.0
52	158982.0
53	152353.0
54	146652.0
55	131358.0
56	116558.5
57	100692.0
58	84310.5
59	78370.0
60	65205.0
61	48311.0
62	37301.5
63	29325.0
64	21548.5
65	13776.5
66	9569.0
67	7089.0
68	5266.0
69	3904.5
70	2959.5
71	2426.0
72	1878.0
73	1509.0
74	1174.5
75	831.0
76	582.5
77	363.5
78	217.0
79	118.0
80	68.5
81	43.5
82	19.0
83	5.5
84	3.5
85	3.5
86	4.0
87	2.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.5
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013747247332544883
2	0.004582415777514961
3	0.0019307931646832702
4	0.029039129196836384
5	0.0024456713419321423
6	0.0
7	7.465733570108645E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009345038917067028
16-17	0.0033853240154113336
18-19	0.0
20-21	6.4359772156109E-5
22-23	7.723172658733081E-4
24-25	1.28719544312218E-5
26-27	7.723172658733081E-5
28-29	0.0
30-31	0.0
32-33	0.0
34-35	3.475427696429886E-4
36-37	0.0
38-39	0.0
40-41	3.7328667850543225E-4
42-43	7.337014025796427E-4
44-45	0.0
46-47	0.0
48-49	5.277501316800938E-4
50-51	0.0
52-53	0.0
54-55	3.6041472407421046E-4
56-57	1.029756354497744E-4
58-59	1.8020736203710523E-4
60-61	7.723172658733081E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0013258113064158455
70-71	7.851892203045299E-4
72-73	0.0
74-75	1.8020736203710523E-4
76-77	0.0028961897470249054
78-79	5.277501316800938E-4
80-81	0.003282348379961559
82-83	0.0019050492558208266
84-85	0.005843867311774698
86-87	0.008341026471431727
88-89	0.00778753243088919
90-91	0.006204282035848908
92-93	0.0091519596005987
94-95	0.001673354076058834
96-97	0.004608159686377404
98-99	0.001995152936839379
100	0.08657676550439783
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3884414.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.16612332884718
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.65573415541716	26.966554636658913
2	15.722575172934352	12.001394861945665
3	5.698953538792129	6.525208908207312
4	2.588418775538296	3.951596408555928
5	1.443543530694507	2.7547230211523024
6	0.8336421741523579	1.9090134018496314
7	0.549929184478624	1.469206555386046
8	0.3783654634185074	1.1552594352165735
9	0.28788802945183367	0.9888813033261726
>10	1.4955739065717981	10.56974857373636
>50	0.15139403519641473	4.030286512950395
>100	0.15737425009591702	12.821330262357902
>500	0.025302438430851656	6.558950630726798
>1k	0.010766995073615879	6.823258538350754
>5k	5.383497536807939E-4	1.4745869495794384
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	9252	0.23818264479532822	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8478	0.21825685933579683	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7706	0.1983825616939904	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	7164	0.18442936309054597	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	7134	0.18365704582467265	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	5944	0.15302179427836476	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	5873	0.1511939767491313	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	5863	0.15093653766050685	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	4854	0.12496093361830124	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4777	0.1229786526358931	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.14878177248872E-5
2	0.0	0.0	0.0	0.0	5.14878177248872E-5
3	0.0	0.0	0.0	0.0	5.14878177248872E-5
4	0.0	0.0	0.0	0.0	5.14878177248872E-5
5	0.0	0.0	0.0	0.0	5.14878177248872E-5
6	0.0	0.0	0.0	0.0	5.14878177248872E-5
7	0.0	0.0	0.0	0.0	5.14878177248872E-5
8	0.0	0.0	0.0	0.0	5.14878177248872E-5
9	0.0	0.0	0.0	0.0	5.14878177248872E-5
10-11	0.0	0.0	0.0	2.57439088624436E-5	7.723172658733081E-5
12-13	0.0	0.0	0.0	2.57439088624436E-5	3.604147240742104E-4
14-15	0.0	0.0	0.0	5.14878177248872E-5	4.5051840509276306E-4
16-17	0.0	0.0	0.0	5.14878177248872E-5	5.020062228176502E-4
18-19	0.0	0.0	0.0	5.14878177248872E-5	5.534940405425374E-4
20-21	0.0	0.0	0.0	7.723172658733081E-5	6.178538126986465E-4
22-23	0.0	0.0	0.0	9.01036810185526E-5	6.950855392859772E-4
24-25	0.0	0.0	0.0	3.475427696429886E-4	6.950855392859772E-4
26-27	0.0	0.0	0.0	6.822135848547554E-4	7.208294481484208E-4
28-29	0.0	0.0	0.0	0.001995152936839379	7.465733570108645E-4
30-31	0.0	0.0	0.0	0.0063716174434547915	7.723172658733081E-4
32-33	0.0	0.0	0.0	0.016527589489688792	8.495489924606388E-4
34-35	0.0	0.0	0.0	0.03327400220470836	9.525246279104133E-4
36-37	0.0	0.0	0.0	0.06213292403950763	9.653965823416351E-4
38-39	0.0	0.0	0.0	0.11085327156168215	9.911404912040787E-4
40-41	0.0	0.0	0.0	0.16739976737803952	0.0010040124456353005
42-43	0.0	0.0	0.0	0.22617311131099826	0.0010040124456353005
44-45	0.0	0.0	0.0	0.29839764762458376	0.001029756354497744
46-47	0.0	0.0	0.0	0.39159059770662963	0.0010683722177914094
48-49	0.0	0.0	0.0	0.49911003307062535	0.001158475898809962
50-51	0.0	0.0	0.0	0.6141338178680233	0.0011842198076724058
52-53	0.0	0.0	0.0	0.7274842485893624	0.00128719544312218
54-55	0.0	0.0	0.0	0.8587009520612375	0.0013129393519846237
56-57	0.0	0.0	0.0	1.0233976090087205	0.0013386832608470673
58-59	0.0	0.0	0.0	1.21766114528472	0.0013644271697095109
60-61	0.0	0.0	0.0	1.4273967707870479	0.0013644271697095109
62-63	0.0	0.0	0.0	1.6441476114543918	0.0015188906228841726
64-65	0.0	0.0	0.0	1.8670383743854284	0.0015961223494715033
66-67	0.0	0.0	0.0	2.1266785672176036	0.0016347382127651687
68-69	0.0	0.0	0.0	2.421922586006538	0.001673354076058834
70-71	0.0	0.0	0.0	2.7530278698408566	0.0016990979849212777
72-73	0.0	0.0	0.0	3.105333262623397	0.0018278175292334958
74-75	0.0	0.0	0.0	3.4494392204332494	0.0019565370735457137
76-77	0.0	0.0	0.0	3.84061791559808	0.001995152936839379
78-79	0.0	0.0	0.0	4.277659384401353	0.002008024891270601
80-81	0.0	0.0	0.0	4.763112273820452	0.0020466407545642663
82-83	0.0	0.0	0.0	5.262363898389822	0.002188232253307706
84-85	0.0	0.0	0.0	5.753866606391595	0.0022139761621701496
86-87	0.0	0.0	0.0	6.266968453928959	0.002329823752051146
88	0.0	0.0	0.0	6.67933438608758	0.0024456713419321423
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8665	0.0	40.05385	1
GTATCAA	13860	0.0	33.62536	1
GGGCACC	3085	0.0	30.646133	94
CAACGCA	16370	0.0	27.818598	5
ATCAACG	16415	0.0	27.69684	3
GTACTAG	2785	0.0	27.01788	1
CTGTGCG	3890	0.0	26.340092	9
TATCAAC	17665	0.0	26.243675	2
AACGCAG	17990	0.0	26.201725	6
TAGTACT	3945	0.0	25.979557	4
TCAACGC	18415	0.0	24.789612	4
CTAACGC	1100	0.0	24.362776	3
CTAGTAC	4395	0.0	24.17654	3
TAACGCC	1185	0.0	23.804285	4
TCTAACG	1190	0.0	23.706404	2
GTACTGT	4545	0.0	23.67891	6
ACGCAGA	19420	0.0	23.401157	7
ACTGTGC	5025	0.0	23.194002	8
CGCAGAG	19805	0.0	22.946249	8
TAGGCAT	3555	0.0	22.870022	5
>>END_MODULE