FastQCFastQC Report
Mon 16 Jan 2017
ERR1146480_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146480_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4239657
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC139080.3280454055599309No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN130910.3087749787305907No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA130540.3079022666220404No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT119590.2820747055717007No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC112600.2655875227642236No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA105760.24945414216291556No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC100780.23770790891810353No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC85380.20138421575141577No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG64960.15321994208493753No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT63850.15060180575928667No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC63350.14942246507205653No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT61220.14439847374445622No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT60210.14201620555625136No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC59000.13916220109315447No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT54570.12871324260429556No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG54180.12779335686825607No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC52760.12444402931652254No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA52650.12418457436533191No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA51770.12210893475580689No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC51500.12147209078470264No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG50250.11852373906662732No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA49500.11675472803578214No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC47380.11175432352192641No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA47020.11090519822712074No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT45620.10760304430287639No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG44960.10604631459573263No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT43530.10267340023025447No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA43230.1019657958179164No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA42810.10097514964064311No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCCCG1950.043.367535
GGGCACC40850.035.48830494
GTACTAG45900.033.508131
GTATCAA91750.032.755081
CAACGCA82800.032.5692985
GGTATCA60100.031.5570851
GCATCGT47200.030.9336288
ATCAACG89500.030.2922253
TAGGCAT55950.029.5576155
TATTCTC25500.029.11015
CTAGGCA62650.027.4471444
TATCAAC109550.027.192562
AACGCAG105200.027.0875666
CTGTGCG57150.026.6174859
AGGGCTA53200.026.140085
ACGCAGA103000.025.9806547
TAGTACT59750.025.9485534
CGCAGAG102500.025.7867728
CTATTGA54550.025.3040949
GCTATTG53650.025.2895188
Produced by FastQC (version 0.11.5)