##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146480_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4239657
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.560015114430247	31.0	31.0	34.0	27.0	34.0
2	30.67911743803803	31.0	31.0	34.0	27.0	34.0
3	30.923079154752376	31.0	31.0	34.0	27.0	34.0
4	34.39179513814443	37.0	35.0	37.0	30.0	37.0
5	34.64480522834748	37.0	35.0	37.0	31.0	37.0
6	34.77283374574877	37.0	35.0	37.0	32.0	37.0
7	34.6494728229194	37.0	35.0	37.0	31.0	37.0
8	34.606865602571155	37.0	35.0	37.0	30.0	37.0
9	36.0134091036138	39.0	35.0	39.0	30.0	39.0
10-11	35.932689130276344	39.0	35.0	39.0	30.0	39.0
12-13	35.928688216994914	39.0	35.0	39.0	30.0	39.0
14-15	36.97990662923911	40.0	36.0	41.0	30.0	41.0
16-17	36.8212304674647	40.0	36.0	41.0	29.5	41.0
18-19	36.77032481637076	40.0	36.0	41.0	29.5	41.0
20-21	37.09735410199457	40.0	36.0	41.0	30.0	41.0
22-23	36.82527784676921	40.0	36.0	41.0	29.5	41.0
24-25	36.94070145297131	40.0	36.0	41.0	30.0	41.0
26-27	37.00583018862139	40.0	36.0	41.0	30.0	41.0
28-29	36.844174540534766	40.0	36.0	41.0	30.0	41.0
30-31	36.789272221785865	40.0	36.0	41.0	30.0	41.0
32-33	36.89201579278701	40.0	36.0	41.0	30.0	41.0
34-35	36.94654166598855	40.0	36.0	41.0	30.0	41.0
36-37	36.88870514760982	40.0	36.0	41.0	30.0	41.0
38-39	36.72105809502986	40.0	35.0	41.0	30.0	41.0
40-41	36.68294817245829	39.0	35.0	41.0	30.0	41.0
42-43	36.533274979556126	39.0	35.0	41.0	29.0	41.0
44-45	36.349685599566186	39.0	35.0	41.0	29.0	41.0
46-47	36.21971659499813	39.0	35.0	41.0	28.5	41.0
48-49	36.09461484738034	39.0	35.0	41.0	28.0	41.0
50-51	35.88229307229335	38.5	34.5	40.5	28.0	41.0
52-53	35.69427031479198	38.0	34.0	40.0	28.0	41.0
54-55	35.50623941983986	38.0	34.0	40.0	28.0	41.0
56-57	35.35767138709571	37.5	34.0	40.0	28.0	41.0
58-59	35.10020456843561	37.0	33.5	40.0	27.0	41.0
60-61	34.847941590557916	36.5	33.0	40.0	27.0	41.0
62-63	34.580904304286875	36.0	33.0	39.5	26.0	41.0
64-65	34.306044922973726	35.5	33.0	39.0	26.0	41.0
66-67	34.0184463035571	35.0	33.0	39.0	26.0	41.0
68-69	33.734237345143725	35.0	32.5	38.5	26.0	40.5
70-71	33.66480389805119	35.0	32.5	38.0	26.0	40.0
72-73	33.45498138174857	35.0	32.0	37.0	26.0	40.0
74-75	33.19446089624703	35.0	32.0	37.0	26.0	39.5
76-77	32.96014571461795	35.0	32.0	36.0	25.0	39.0
78-79	32.73969521591015	35.0	32.0	36.0	25.0	39.0
80-81	32.5072856129635	35.0	32.0	36.0	25.0	38.0
82-83	32.26981498739167	35.0	32.0	35.0	25.0	37.0
84-85	32.0826470160204	35.0	31.5	35.0	25.0	37.0
86-87	31.90466834934996	35.0	31.5	35.0	24.0	36.5
88-89	31.897025514092295	35.0	32.0	35.0	25.0	36.0
90-91	31.81443133253468	35.0	32.0	35.0	24.5	36.0
92-93	31.667758382340836	35.0	32.0	35.0	24.0	36.0
94-95	31.648918178994197	35.0	32.0	35.0	24.0	35.5
96-97	31.55656471738162	35.0	32.0	35.0	24.0	35.0
98-99	31.477793132793526	35.0	32.0	35.0	24.0	35.0
100	30.999935607998477	34.0	31.0	35.0	22.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7241975947615238
1101	2	0.8700608062072561
1101	3	0.849014359106036
1101	4	0.8665891101316205
1101	5	0.809100173394576
1101	6	0.5585316195074412
1101	7	0.6290070387615216
1101	8	0.6138877976800856
1101	9	0.806642981653745
1101	10-11	0.8579868991565363
1101	12-13	0.7483936136136151
1101	14-15	0.9626675613924647
1101	16-17	1.0229428775372327
1101	18-19	1.1669455263777095
1101	20-21	0.9301614560925628
1101	22-23	1.178294242201119
1101	24-25	1.0244713593156831
1101	26-27	0.9514618308155391
1101	28-29	1.130566808723792
1101	30-31	1.1531019487029681
1101	32-33	1.0170450896783834
1101	34-35	0.9713147914103502
1101	36-37	0.9105991808352414
1101	38-39	1.005463899374348
1101	40-41	0.9469081580756082
1101	42-43	0.9445111270276456
1101	44-45	1.0089988206020166
1101	46-47	0.986108663122522
1101	48-49	0.9776616660604702
1101	50-51	0.973122134861022
1101	52-53	1.0272704109611723
1101	54-55	1.031523292917285
1101	56-57	0.8690480471421225
1101	58-59	0.8532770423578384
1101	60-61	0.9247264397586363
1101	62-63	0.7871464768623682
1101	64-65	0.8275349730485857
1101	66-67	0.8415773831797253
1101	68-69	0.9010657955295329
1101	70-71	0.8045786598171745
1101	72-73	0.7597956871904543
1101	74-75	0.7827140969542938
1101	76-77	0.7521499867032446
1101	78-79	0.717532108881251
1101	80-81	0.7290929979002456
1101	82-83	0.6880282828692614
1101	84-85	0.7416866595242269
1101	86-87	0.6702290583468127
1101	88-89	0.7169614759496383
1101	90-91	0.7084317876923194
1101	92-93	0.7863364871314928
1101	94-95	0.7054161001053565
1101	96-97	0.6994163294453699
1101	98-99	0.6777446895933075
1101	100	0.8400271178164616
1102	1	0.5854258667920114
1102	2	0.7344889393821887
1102	3	0.7287575814922782
1102	4	0.8107114284066128
1102	5	0.6993297608215769
1102	6	0.47192703977174943
1102	7	0.4823120653942894
1102	8	0.5337674497949507
1102	9	0.714454248722312
1102	10-11	0.6841754036618894
1102	12-13	0.6449400190197707
1102	14-15	0.8850440248702967
1102	16-17	1.0476338640148413
1102	18-19	1.0682526792235265
1102	20-21	0.8501142166885245
1102	22-23	1.1230060797635844
1102	24-25	0.9519837694297948
1102	26-27	0.8338680776571792
1102	28-29	0.9553694752317767
1102	30-31	1.036412849799376
1102	32-33	0.8824896127534885
1102	34-35	0.7479233081843688
1102	36-37	0.7073450267845445
1102	38-39	0.7252192974068095
1102	40-41	0.6691103265864129
1102	42-43	0.7589016239969553
1102	44-45	0.8040346561447365
1102	46-47	0.7677197389461767
1102	48-49	0.6711784251685771
1102	50-51	0.756911297383148
1102	52-53	0.7038500306726903
1102	54-55	0.7704941757709491
1102	56-57	0.6839079349403292
1102	58-59	0.6730803224006863
1102	60-61	0.7339064785951237
1102	62-63	0.6879003613065677
1102	64-65	0.5918871639039835
1102	66-67	0.6567829310112572
1102	68-69	0.6570825577975867
1102	70-71	0.5967169903662963
1102	72-73	0.539363542756405
1102	74-75	0.5903569111616349
1102	76-77	0.5472807849047783
1102	78-79	0.6353865397835534
1102	80-81	0.6782089641807545
1102	82-83	0.6715685605611554
1102	84-85	0.6225350283380067
1102	86-87	0.578730381504144
1102	88-89	0.5740433317138809
1102	90-91	0.5765019467568564
1102	92-93	0.6106621410239086
1102	94-95	0.6459556098548482
1102	96-97	0.6545653127495896
1102	98-99	0.6743778822492708
1102	100	0.7031115919361852
1103	1	0.5617576631093968
1103	2	0.730712460082124
1103	3	0.6575093876415856
1103	4	0.6659627087346749
1103	5	0.6120569249338104
1103	6	0.4894452381650396
1103	7	0.5531969758802262
1103	8	0.5862485420096846
1103	9	0.7160993411610619
1103	10-11	0.7855458991784019
1103	12-13	0.6571840374141757
1103	14-15	0.8429343180234241
1103	16-17	1.0504977292054818
1103	18-19	1.1166094784967058
1103	20-21	0.8182062882997769
1103	22-23	1.0514503333774314
1103	24-25	0.9303095048016985
1103	26-27	0.842154024851034
1103	28-29	1.0115288644079428
1103	30-31	1.0432119363016312
1103	32-33	0.8810455644899378
1103	34-35	0.8183191115707871
1103	36-37	0.7931939885209331
1103	38-39	0.8508164001006264
1103	40-41	0.7716307962785791
1103	42-43	0.8191264265806311
1103	44-45	0.889240087349279
1103	46-47	0.7700012057696526
1103	48-49	0.7390525765428677
1103	50-51	0.7717922626540528
1103	52-53	0.8412648694104732
1103	54-55	0.8129505014938729
1103	56-57	0.6804971907632549
1103	58-59	0.6805819932054717
1103	60-61	0.6742031786778782
1103	62-63	0.664401353833064
1103	64-65	0.6604156360354594
1103	66-67	0.6828271746687307
1103	68-69	0.6880457378649183
1103	70-71	0.6290051030725081
1103	72-73	0.6366332672758546
1103	74-75	0.6428833297900098
1103	76-77	0.6226039502864467
1103	78-79	0.6004731291638308
1103	80-81	0.6551869543612767
1103	82-83	0.6187046206976845
1103	84-85	0.591590528926055
1103	86-87	0.6406151488671341
1103	88-89	0.6940772456285487
1103	90-91	0.6257535631127027
1103	92-93	0.6608571242536243
1103	94-95	0.5875961099139637
1103	96-97	0.6250583152822387
1103	98-99	0.5903732276422637
1103	100	0.7078515499949383
1104	1	0.5933145747458397
1104	2	0.7694774043852242
1104	3	0.7006763153638822
1104	4	0.8392161499911239
1104	5	0.7174265344368393
1104	6	0.4481295660750533
1104	7	0.5065272550135802
1104	8	0.5333764532326626
1104	9	0.6390939782031992
1104	10-11	0.7379055704595672
1104	12-13	0.6744074557641255
1104	14-15	0.8421019097022366
1104	16-17	0.957574684783765
1104	18-19	0.9780478135351984
1104	20-21	0.8786257035955245
1104	22-23	1.0737615660127062
1104	24-25	0.916438765063603
1104	26-27	0.8008127119675592
1104	28-29	1.0100767915055187
1104	30-31	1.0538257641014965
1104	32-33	0.8752067308804641
1104	34-35	0.7508968261720952
1104	36-37	0.7563760665237638
1104	38-39	0.8367563361797181
1104	40-41	0.7069818813947819
1104	42-43	0.7367448386984421
1104	44-45	0.7888632739180395
1104	46-47	0.7483950104517589
1104	48-49	0.7741639009248615
1104	50-51	0.8722087686130635
1104	52-53	0.8904352436706944
1104	54-55	0.8307770458718267
1104	56-57	0.7652268548314325
1104	58-59	0.7689338660155443
1104	60-61	0.7568155457734065
1104	62-63	0.6629273945094596
1104	64-65	0.718305799519662
1104	66-67	0.8539281896168092
1104	68-69	0.7759426918135048
1104	70-71	0.6484282335067633
1104	72-73	0.7058111261368083
1104	74-75	0.7045137172690019
1104	76-77	0.6674871814610199
1104	78-79	0.5980621823316525
1104	80-81	0.6554888131377226
1104	82-83	0.5607977617250697
1104	84-85	0.667381056680945
1104	86-87	0.6064924074510358
1104	88-89	0.5132063157574436
1104	90-91	0.5421223607779879
1104	92-93	0.48750970297020046
1104	94-95	0.4906191039353338
1104	96-97	0.5612864628133778
1104	98-99	0.6198695079767838
1104	100	0.7167076675997457
1105	1	0.43775086984090805
1105	2	0.6236517838431226
1105	3	0.6407596578284007
1105	4	0.7290054754476571
1105	5	0.6003329786623866
1105	6	0.33848275553418716
1105	7	0.4203033522890536
1105	8	0.4764248582054478
1105	9	0.5017507229736324
1105	10-11	0.5569400631432018
1105	12-13	0.49901636719648224
1105	14-15	0.6909417288617732
1105	16-17	0.8803337644069629
1105	18-19	0.769431463983814
1105	20-21	0.6141723219573336
1105	22-23	0.8920648153907109
1105	24-25	0.7782100760538242
1105	26-27	0.5759628869785587
1105	28-29	0.7713343299012294
1105	30-31	0.8237831884245352
1105	32-33	0.6734062252896962
1105	34-35	0.5493594080043209
1105	36-37	0.5708181972595483
1105	38-39	0.6394284118344515
1105	40-41	0.5160056611851687
1105	42-43	0.6045869793056582
1105	44-45	0.6168460777769411
1105	46-47	0.5443461185379093
1105	48-49	0.6615957292370211
1105	50-51	0.6485913980562472
1105	52-53	0.6686843537396427
1105	54-55	0.5919611545740082
1105	56-57	0.5296125219350927
1105	58-59	0.5124868874466344
1105	60-61	0.5543575711435338
1105	62-63	0.5899730691333573
1105	64-65	0.5893197583142964
1105	66-67	0.6586845160304762
1105	68-69	0.6011338959628034
1105	70-71	0.5163967967578529
1105	72-73	0.4667113898634767
1105	74-75	0.45271874742163476
1105	76-77	0.47290254531500864
1105	78-79	0.5112713382112233
1105	80-81	0.5637588240540339
1105	82-83	0.4721361476391692
1105	84-85	0.446233727937944
1105	86-87	0.5461751303400391
1105	88-89	0.5407439122952127
1105	90-91	0.5698838703536993
1105	92-93	0.5077664958762966
1105	94-95	0.5008831255075954
1105	96-97	0.4537456705393055
1105	98-99	0.5118127829919707
1105	100	0.6540253959112583
1106	1	0.4156813179992618
1106	2	0.514842473537005
1106	3	0.5472070610054551
1106	4	0.680557595879975
1106	5	0.590294849556237
1106	6	0.3431011258092198
1106	7	0.3615953612945262
1106	8	0.3970425613907551
1106	9	0.5529037918542201
1106	10-11	0.5564609822509468
1106	12-13	0.4497343873922546
1106	14-15	0.5893896977080715
1106	16-17	0.7569927781117087
1106	18-19	0.6791233944717376
1106	20-21	0.5528093995519896
1106	22-23	0.8195888732960981
1106	24-25	0.6375051750512881
1106	26-27	0.5253290743619701
1106	28-29	0.7384652334042698
1106	30-31	0.7468879911491726
1106	32-33	0.6607214379233568
1106	34-35	0.6182499927898846
1106	36-37	0.482156329972554
1106	38-39	0.6064778813469403
1106	40-41	0.5396153760781104
1106	42-43	0.47492650821698845
1106	44-45	0.48423825809020116
1106	46-47	0.44977129360432855
1106	48-49	0.5125968340299778
1106	50-51	0.5410411779487774
1106	52-53	0.5179343479960536
1106	54-55	0.5389981227011873
1106	56-57	0.4998846157409602
1106	58-59	0.49812365042348716
1106	60-61	0.5525916165511049
1106	62-63	0.5475511263095214
1106	64-65	0.5464683973095887
1106	66-67	0.5682295058402076
1106	68-69	0.49528857578652463
1106	70-71	0.4740410986580983
1106	72-73	0.40585902203419266
1106	74-75	0.49598239701906266
1106	76-77	0.5048665472296179
1106	78-79	0.5103849921356982
1106	80-81	0.48314014951751716
1106	82-83	0.50587767465327
1106	84-85	0.49588949940091
1106	86-87	0.4857514664627942
1106	88-89	0.46560671786463104
1106	90-91	0.4365998902325998
1106	92-93	0.5214683689056869
1106	94-95	0.5183119398079832
1106	96-97	0.4947906992149953
1106	98-99	0.5217307192896534
1106	100	0.4726065351038642
1107	1	0.38595223256234945
1107	2	0.5572141687782768
1107	3	0.47086210690946473
1107	4	0.5788305311012891
1107	5	0.5059729724755044
1107	6	0.28390526355138235
1107	7	0.27506043996209684
1107	8	0.31784765082478117
1107	9	0.3737732775531839
1107	10-11	0.4504056973303463
1107	12-13	0.31863599420413635
1107	14-15	0.5276186131085936
1107	16-17	0.6146037127487389
1107	18-19	0.7422476964819538
1107	20-21	0.4571106279441892
1107	22-23	0.7122516896827449
1107	24-25	0.4165137494692317
1107	26-27	0.4563567072820831
1107	28-29	0.5611824207150278
1107	30-31	0.5803320953657192
1107	32-33	0.5224682648512129
1107	34-35	0.3391458187135754
1107	36-37	0.3491524472326262
1107	38-39	0.3770617954973545
1107	40-41	0.21964890401962833
1107	42-43	0.4295789958383054
1107	44-45	0.2646833985805159
1107	46-47	0.2950692784491551
1107	48-49	0.2962970399235161
1107	50-51	0.3346960342124774
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2210	22-23	-1.7471999012624124
2210	24-25	-0.884337904316979
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2210	30-31	0.053353820310519495
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2211	88-89	-0.4456929150899924
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2213	68-69	-0.5680157074935792
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2214	3	-1.0545301800706
2214	4	-0.8116361929444906
2214	5	-1.1081150749253794
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2214	9	-4.619216813307904
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2214	14-15	-3.7677644886087407
2214	16-17	-3.2913883200077407
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2214	26-27	-1.5140669770147
2214	28-29	-1.0060315960419501
2214	30-31	-1.045479759407165
2214	32-33	-1.2536863677802756
2214	34-35	-1.1543850458552072
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2214	40-41	-1.2969700415430836
2214	42-43	-3.9182112718739788
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2214	52-53	-1.159497759519013
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2214	80-81	-1.0743341143893872
2214	82-83	-1.1637208026134687
2214	84-85	-1.1629045251099228
2214	86-87	-0.983455784353545
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2214	90-91	-0.9805490383587703
2214	92-93	-0.9899177903800869
2214	94-95	-1.0699791356241093
2214	96-97	-0.975167755756587
2214	98-99	-1.2192934390244012
2214	100	-1.0442610278907942
2215	1	-4.008206659461738
2215	2	-2.170760044709265
2215	3	-3.6982464725972477
2215	4	-1.9389065231686118
2215	5	-1.5105472325186469
2215	6	-1.327337306431751
2215	7	-1.182493426596558
2215	8	-1.1508194220180314
2215	9	-1.7563529660776211
2215	10-11	-1.326790824984947
2215	12-13	-1.240113323635505
2215	14-15	-3.610237434888262
2215	16-17	-3.3982915970513403
2215	18-19	-2.2676376098123043
2215	20-21	-1.7910789837037626
2215	22-23	-3.1833322112193514
2215	24-25	-5.53895217881912
2215	26-27	-3.4724737209841408
2215	28-29	-1.650545438997625
2215	30-31	-3.9739913660719424
2215	32-33	-4.040646901970327
2215	34-35	-2.9611351122239995
2215	36-37	-1.8744297767746545
2215	38-39	-1.661602883402253
2215	40-41	-1.8141286022176715
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2215	44-45	-3.3025782821037666
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2215	50-51	-5.092689235208248
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2215	54-55	-3.116440918231909
2215	56-57	-1.9394173013493798
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2215	60-61	-3.12792125004097
2215	62-63	-2.7260774903361806
2215	64-65	-1.7969707711589962
2215	66-67	-1.6488517284714703
2215	68-69	-1.7281686215630572
2215	70-71	-1.3828902234791656
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2215	80-81	-1.594445951432622
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2215	84-85	-1.382928710348601
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2215	88-89	-1.3372127440515342
2215	90-91	-1.7028786055442389
2215	92-93	-3.9946451608709452
2215	94-95	-2.0853391694609478
2215	96-97	-1.7190339742651588
2215	98-99	-3.133892436518135
2215	100	-3.207473232549656
2216	1	-0.5570504533608123
2216	2	-0.4697965032321463
2216	3	-0.7681602211506231
2216	4	-0.701313848354765
2216	5	-0.7847683128089145
2216	6	-0.6503085777853528
2216	7	-0.8033150353134104
2216	8	-0.7434413932093236
2216	9	-0.8685533605779057
2216	10-11	-0.8522107639902501
2216	12-13	-1.0256275933368642
2216	14-15	-1.3171475950903186
2216	16-17	-1.1318911574237092
2216	18-19	-3.0824642698797007
2216	20-21	-1.3868937141406974
2216	22-23	-0.8417356496314525
2216	24-25	-0.9809924440514166
2216	26-27	-0.997689567958794
2216	28-29	-1.025023235618903
2216	30-31	-1.1465299972578435
2216	32-33	-0.997691789115585
2216	34-35	-1.2226015323886372
2216	36-37	-1.3071904781408463
2216	38-39	-1.3643207137962392
2216	40-41	-1.402778275772313
2216	42-43	-1.4033736913755561
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2216	46-47	-1.4434075761361314
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2216	50-51	-1.2900276684161511
2216	52-53	-1.2804892665130083
2216	54-55	-1.2208553182202593
2216	56-57	-1.2386291742067428
2216	58-59	-1.326328221800182
2216	60-61	-1.2859648343162817
2216	62-63	-1.2522193939820312
2216	64-65	-1.2489611051740894
2216	66-67	-1.2266522074035606
2216	68-69	-1.383442988506559
2216	70-71	-1.4036736195297976
2216	72-73	-1.3421534706526188
2216	74-75	-1.3897332854911397
2216	76-77	-1.3026461249614627
2216	78-79	-1.3797353956788285
2216	80-81	-1.3505740257326053
2216	82-83	-1.5293918701735905
2216	84-85	-1.419961660584793
2216	86-87	-1.3710732624121853
2216	88-89	-1.2750247959625334
2216	90-91	-1.3531444929151348
2216	92-93	-1.2691503978258716
2216	94-95	-1.1853895373943324
2216	96-97	-1.189241705597361
2216	98-99	-1.1469025756122413
2216	100	-1.1709640505918593
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	17298.0
1	3060.0
2	434.0
3	463.0
4	432.0
5	240.0
6	233.0
7	319.0
8	542.0
9	899.0
10	1399.0
11	2567.0
12	4193.0
13	5829.0
14	7811.0
15	10111.0
16	12355.0
17	14477.0
18	16978.0
19	19474.0
20	22628.0
21	26172.0
22	30187.0
23	35119.0
24	40285.0
25	45373.0
26	52284.0
27	60119.0
28	71608.0
29	85142.0
30	102051.0
31	122740.0
32	152261.0
33	195848.0
34	274263.0
35	374322.0
36	552190.0
37	863590.0
38	741783.0
39	272285.0
40	293.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.873430110291046	21.574403272160595	13.024411360599272	23.52775525694909
2	15.10950997720714	19.89904326852453	41.534750818600116	23.456695935668222
3	18.281011323169537	27.16359046981377	29.14248469801919	25.412913508997505
4	11.943017336985886	17.303667402864537	40.886304823833854	29.867010436315727
5	13.016573564737822	38.678118440815126	35.26343344751942	13.041874546927637
6	29.02831888318215	39.05425638903367	18.72615393221169	13.19127079557249
7	26.504540017153964	31.983971612980312	24.170002555552447	17.341485814313277
8	25.65779678969942	35.308967960528875	20.332575343063297	18.700659906708403
9	25.908723503789062	15.36961027253584	22.049907327428304	36.67175889624679
10-11	25.284297932613654	26.135964545133977	27.95296951576809	20.62676800648428
12-13	25.62316635570399	23.997441790537945	27.809429994507962	22.569961859250103
14-15	23.15662698027837	25.13379305962064	25.291506319319673	26.418073640781316
16-17	21.95073568975702	27.68582429812041	26.694521863907056	23.66891814821551
18-19	21.40640022821795	28.3807421897185	28.260621863085117	21.95223571897843
20-21	23.23992616208227	27.432915875685858	27.085625354733477	22.241532607498396
22-23	23.670568201158563	27.033040177003148	26.896006857117932	22.400384764720354
24-25	23.034845005917486	27.250631408191435	27.551355046677134	22.16316853921394
26-27	22.930871778434454	27.47747560862709	26.547031280060622	23.044621332877828
28-29	22.020699007299765	27.994872133490496	27.16049133913826	22.823937520071482
30-31	22.859531106481914	27.449288399245653	27.076820067174467	22.614360427097967
32-33	23.340298720504297	27.794834927911445	26.072922932689607	22.791943418894654
34-35	23.295540506508615	27.621527588399974	27.01017257185514	22.072759333236267
36-37	22.936635503955287	27.25284535548948	26.79432254722613	23.01619659332911
38-39	23.11595018325373	27.164642826270676	27.38808799167215	22.331318998803447
40-41	22.73627155883145	26.37650833110694	28.014435818322802	22.87278429173881
42-43	22.647865799068096	26.649141251494413	27.390862609488387	23.3121303399491
44-45	22.784619779797023	27.630349497308625	27.089857098487	22.495173624407354
46-47	22.80222627696542	27.25723503250686	26.647620231265833	23.292918459261887
48-49	23.499615758797535	26.720223322267284	27.00946530364777	22.770695615287416
50-51	23.099609119092097	26.74991388525425	26.821331319147472	23.329145676506176
52-53	22.448413626229577	27.37331228886567	26.72255733913953	23.45571674576522
54-55	22.56610047820508	27.268195514246845	26.669955964437165	23.495748043110908
56-57	23.563314738957487	26.652727800864312	26.5009099585628	23.283047501615396
58-59	22.57799725133713	27.00949582276045	27.375932825534605	23.03657410036781
60-61	22.59801058658743	27.623912702743013	26.649136173259038	23.128940537410518
62-63	23.046609735463385	26.903597591741814	26.23409953982086	23.81569313297394
64-65	22.123262560760278	27.071030893658072	26.800434528769628	24.005272016812025
66-67	22.94718420909106	26.641432165630018	26.724771229586135	23.686612395692784
68-69	22.307895826936868	26.786554371114907	26.95484590313708	23.950703898811142
70-71	22.33714934596276	26.411920918176484	27.153184068907105	24.097745666953646
72-73	22.917994963135364	26.62739118561732	27.419193231311468	23.03542061993585
74-75	23.086066845995816	26.908881962670034	26.983440392503656	23.021610798830494
76-77	22.64734468589807	26.909678133757676	26.708036021475927	23.734941158868327
78-79	22.499423628116322	26.813614615462704	26.828083210073554	23.858878546347416
80-81	22.115230026107806	27.107381086188948	27.535859696049208	23.24152919165404
82-83	22.918609106886933	26.1529321322848	28.465127605873462	22.463331154954798
84-85	23.214753655557214	26.66841601888067	26.98998695455961	23.126843371002508
86-87	21.88062705568361	27.3000254654098	27.547447910391256	23.271899568515334
88-89	22.903467029231813	26.01766312450058	27.413312342719475	23.66555750354813
90-91	22.609991052436442	26.06911936202429	27.60011510313668	23.720774482402586
92-93	22.737085054348217	26.37344500472002	27.518497282708505	23.37097265822326
94-95	21.606565115145564	26.341496516051137	28.127244662570305	23.924693706232993
96-97	21.956504318277002	26.230420360630745	27.716035484951142	24.097039836141114
98-99	21.36285947904731	26.931410809296715	27.604468320578235	24.101261391077742
100	22.12676228982373	26.147232252854934	26.928627947761957	24.797377509559375
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	14615.0
1	8616.5
2	2332.0
3	1580.0
4	813.5
5	424.0
6	336.0
7	328.5
8	329.5
9	325.0
10	305.5
11	287.5
12	284.5
13	286.5
14	302.5
15	399.0
16	471.5
17	528.0
18	697.5
19	900.5
20	1167.0
21	1550.0
22	2149.5
23	2951.0
24	4164.5
25	5772.0
26	8140.0
27	10948.0
28	15394.0
29	24244.5
30	35779.5
31	47386.0
32	62772.0
33	77825.5
34	89475.0
35	102257.0
36	115115.0
37	137159.5
38	146776.5
39	136234.5
40	126445.0
41	117408.0
42	114801.0
43	120499.0
44	130713.0
45	153333.5
46	176180.5
47	192454.0
48	202278.5
49	196846.0
50	186284.5
51	187130.0
52	186549.5
53	177999.5
54	168263.5
55	145636.0
56	126800.0
57	104472.5
58	81534.0
59	75770.0
60	62377.0
61	43330.5
62	32347.5
63	26738.5
64	19166.5
65	9415.0
66	5413.0
67	3865.5
68	2794.5
69	2111.0
70	1641.5
71	1183.5
72	899.5
73	740.0
74	572.5
75	438.5
76	320.0
77	199.5
78	136.0
79	97.5
80	67.5
81	49.0
82	42.5
83	30.0
84	21.5
85	23.5
86	20.0
87	12.5
88	12.0
89	12.5
90	9.5
91	11.0
92	11.5
93	10.0
94	7.5
95	3.0
96	1.0
97	1.5
98	1.5
99	1.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9979109159066406
2	0.4490221732559969
3	0.4819257784297173
4	0.46647641542700274
5	0.4358135575590195
6	0.4875394401009327
7	0.5045455328107911
8	0.5067626933027837
9	0.5217403200306062
10-11	0.5351848038650296
12-13	0.5341233972465226
14-15	0.5309391773910012
16-17	0.5361282764148138
18-19	0.5338993225159488
20-21	0.5326492213874848
22-23	0.49473341829303646
24-25	0.5306089619985768
26-27	0.5099115329376881
28-29	0.5169875770610689
30-31	0.5185089265475957
32-33	0.5221177090505199
34-35	0.5337931818540981
36-37	0.5519314416236973
38-39	0.5416122106104337
40-41	0.5472258722816492
42-43	0.5451856128927411
44-45	0.5543255032187745
46-47	0.5535825185858195
48-49	0.524900953072383
50-51	0.5401262413445238
52-53	0.5320005840095083
54-55	0.5413527556592432
56-57	0.5290994059189222
58-59	0.5353617049681142
60-61	0.5291465795464114
62-63	0.5228960739040918
64-65	0.5348310016588605
66-67	0.5318118894995515
68-69	0.5533820306689905
70-71	0.5362108302629198
72-73	0.5412348215905202
74-75	0.538486957789274
76-77	0.525608557484721
78-79	0.5573328219712114
80-81	0.5313401532246594
82-83	0.5295947290075589
84-85	0.5391473885741228
86-87	0.8471322090442693
88-89	1.1158213978159082
90-91	1.1061154239600044
92-93	1.1057262415332183
94-95	1.1102902899927989
96-97	1.1034147337862474
98-99	1.099794157876451
100	1.0942158764258523
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4239657.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.045279785775953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.5097927413777	22.086222899090945
2	13.048554585296158	7.8409494663038535
3	5.165242894133711	4.655735037472153
4	2.6012300505458117	3.1261873862326808
5	1.481012774816935	2.22487215928416
6	0.9047791756138405	1.631060608539693
7	0.6034073996778908	1.2690680903690834
8	0.4125316410820797	0.9915702861437111
9	0.28440059659873196	0.7690405946445451
>10	1.5048576747151183	8.342858824158988
>50	0.18600119912246166	3.9686493839646966
>100	0.2239364191180222	14.90960648477578
>500	0.04505405358939612	9.611115598359246
>1k	0.027550475278392512	14.646713336887649
>5k	0.0010988793558333197	1.9471267206702743
>10k+	5.494396779166599E-4	1.9792231231026882
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	13908	0.3280454055599309	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	13091	0.3087749787305907	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	13054	0.3079022666220404	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	11959	0.2820747055717007	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	11260	0.2655875227642236	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	10576	0.24945414216291556	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	10078	0.23770790891810353	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	8538	0.20138421575141577	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	6496	0.15321994208493753	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	6385	0.15060180575928667	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6335	0.14942246507205653	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6122	0.14439847374445622	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6021	0.14201620555625136	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5900	0.13916220109315447	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	5457	0.12871324260429556	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	5418	0.12779335686825607	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5276	0.12444402931652254	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	5265	0.12418457436533191	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5177	0.12210893475580689	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	5150	0.12147209078470264	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5025	0.11852373906662732	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4950	0.11675472803578214	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	4738	0.11175432352192641	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4702	0.11090519822712074	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4562	0.10760304430287639	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	4496	0.10604631459573263	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	4353	0.10267340023025447	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	4323	0.1019657958179164	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	4281	0.10097514964064311	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	2.3586813744602454E-5	0.0
2	0.0	0.0	0.0	9.434725497840982E-5	0.0
3	0.0	0.0	0.0	9.434725497840982E-5	0.0
4	0.0	0.0	0.0	9.434725497840982E-5	0.0
5	0.0	0.0	0.0	9.434725497840982E-5	0.0
6	0.0	0.0	0.0	9.434725497840982E-5	0.0
7	0.0	0.0	0.0	1.1793406872301227E-4	0.0
8	0.0	0.0	0.0	1.1793406872301227E-4	0.0
9	0.0	0.0	0.0	1.1793406872301227E-4	0.0
10-11	0.0	0.0	0.0	1.1793406872301227E-4	0.0
12-13	0.0	0.0	0.0	1.1793406872301227E-4	2.3586813744602454E-5
14-15	0.0	0.0	0.0	1.1793406872301227E-4	2.3586813744602454E-5
16-17	0.0	0.0	0.0	1.1793406872301227E-4	2.3586813744602454E-5
18-19	0.0	0.0	0.0	1.1793406872301227E-4	2.3586813744602454E-5
20-21	0.0	0.0	0.0	1.2972747559531348E-4	2.3586813744602454E-5
22-23	0.0	0.0	0.0	2.3586813744602453E-4	2.3586813744602454E-5
24-25	0.0	0.0	0.0	3.773890199136392E-4	2.3586813744602454E-5
26-27	0.0	0.0	0.0	6.486373779765674E-4	2.3586813744602454E-5
28-29	0.0	0.0	0.0	0.0016274901483775692	2.3586813744602454E-5
30-31	0.0	0.0	0.0	0.005424967161258565	2.3586813744602454E-5
32-33	0.0	0.0	0.0	0.014753551997248834	2.3586813744602454E-5
34-35	0.0	0.0	0.0	0.030309055661814154	2.3586813744602454E-5
36-37	0.0	0.0	0.0	0.059250076126441356	2.3586813744602454E-5
38-39	0.0	0.0	0.0	0.10667136515996459	2.3586813744602454E-5
40-41	0.0	0.0	0.0	0.15865670265306842	2.3586813744602454E-5
42-43	0.0	0.0	0.0	0.21507636113015746	2.3586813744602454E-5
44-45	0.0	0.0	0.0	0.2844687671667779	2.3586813744602454E-5
46-47	0.0	0.0	0.0	0.37609174515768606	2.3586813744602454E-5
48-49	0.0	0.0	0.0	0.48374196308805173	4.717362748920491E-5
50-51	0.0	0.0	0.0	0.5989753416373069	4.717362748920491E-5
52-53	0.0	0.0	0.0	0.7136780168773087	4.717362748920491E-5
54-55	0.0	0.0	0.0	0.8427096814671564	4.717362748920491E-5
56-57	0.0	0.0	0.0	1.0018499138019892	4.717362748920491E-5
58-59	0.0	0.0	0.0	1.1982101382258046	4.717362748920491E-5
60-61	0.0	0.0	0.0	1.4079440860428094	4.717362748920491E-5
62-63	0.0	0.0	0.0	1.6284100341136087	4.717362748920491E-5
64-65	0.0	0.0	0.0	1.8582989142753765	4.717362748920491E-5
66-67	0.0	0.0	0.0	2.122990138117305	4.717362748920491E-5
68-69	0.0	0.0	0.0	2.42501928811694	4.717362748920491E-5
70-71	0.0	0.0	0.0	2.7572395597096655	4.717362748920491E-5
72-73	0.0	0.0	0.0	3.1061357086198247	5.896703436150613E-5
74-75	0.0	0.0	0.0	3.4483096156127724	7.076044123380735E-5
76-77	0.0	0.0	0.0	3.844025589805968	7.076044123380735E-5
78-79	0.0	0.0	0.0	4.277103076970613	7.076044123380735E-5
80-81	0.0	0.0	0.0	4.748155334264069	7.076044123380735E-5
82-83	0.0	0.0	0.0	5.242322669027235	7.076044123380735E-5
84-85	0.0	0.0	0.0	5.721783625420642	7.076044123380735E-5
86-87	0.0	0.0	0.0	6.246790719154875	7.076044123380735E-5
88	0.0	0.0	0.0	6.660774680593265	7.076044123380735E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCCG	195	0.0	43.36753	5
GGGCACC	4085	0.0	35.488304	94
GTACTAG	4590	0.0	33.50813	1
GTATCAA	9175	0.0	32.75508	1
CAACGCA	8280	0.0	32.569298	5
GGTATCA	6010	0.0	31.557085	1
GCATCGT	4720	0.0	30.933628	8
ATCAACG	8950	0.0	30.292225	3
TAGGCAT	5595	0.0	29.557615	5
TATTCTC	2550	0.0	29.1101	5
CTAGGCA	6265	0.0	27.447144	4
TATCAAC	10955	0.0	27.19256	2
AACGCAG	10520	0.0	27.087566	6
CTGTGCG	5715	0.0	26.617485	9
AGGGCTA	5320	0.0	26.14008	5
ACGCAGA	10300	0.0	25.980654	7
TAGTACT	5975	0.0	25.948553	4
CGCAGAG	10250	0.0	25.786772	8
CTATTGA	5455	0.0	25.304094	9
GCTATTG	5365	0.0	25.289518	8
>>END_MODULE