##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146479_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5324780
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.478201916323304	31.0	30.0	34.0	27.0	34.0
2	30.50437088480651	31.0	30.0	34.0	26.0	34.0
3	30.773891879101107	31.0	31.0	34.0	27.0	34.0
4	34.24168716829615	37.0	35.0	37.0	30.0	37.0
5	34.49114761548834	37.0	35.0	37.0	30.0	37.0
6	34.599028880066406	37.0	35.0	37.0	31.0	37.0
7	34.51626001449825	37.0	35.0	37.0	30.0	37.0
8	34.47643808758296	37.0	35.0	37.0	30.0	37.0
9	35.867295550238694	39.0	35.0	39.0	30.0	39.0
10-11	35.78306765725532	39.0	35.0	39.0	30.0	39.0
12-13	35.76582788396891	39.0	35.0	39.0	30.0	39.0
14-15	36.78098747741691	40.0	36.0	41.0	29.5	41.0
16-17	36.61257535522594	40.0	36.0	41.0	28.5	41.0
18-19	36.54795794380237	40.0	36.0	41.0	28.0	41.0
20-21	36.887095335394136	40.0	36.0	41.0	30.0	41.0
22-23	36.595638317451616	40.0	36.0	41.0	28.5	41.0
24-25	36.705468488838974	40.0	36.0	41.0	29.5	41.0
26-27	36.773149313211064	40.0	36.0	41.0	30.0	41.0
28-29	36.601150094463996	40.0	35.5	41.0	29.5	41.0
30-31	36.546967574247205	39.0	35.0	41.0	29.5	41.0
32-33	36.65305327168446	39.5	35.0	41.0	30.0	41.0
34-35	36.69478081723564	40.0	35.0	41.0	30.0	41.0
36-37	36.62488684978534	39.5	35.0	41.0	30.0	41.0
38-39	36.45172316978354	39.0	35.0	41.0	29.0	41.0
40-41	36.40784755426515	39.0	35.0	41.0	29.0	41.0
42-43	36.242821299659326	39.0	35.0	41.0	28.0	41.0
44-45	36.033541010145015	39.0	35.0	41.0	28.0	41.0
46-47	35.89354236231356	38.5	34.5	40.5	27.5	41.0
48-49	35.754758600355316	38.0	34.0	40.0	27.5	41.0
50-51	35.519833964971326	38.0	34.0	40.0	27.0	41.0
52-53	35.31847916721442	38.0	34.0	40.0	27.0	41.0
54-55	35.11094608603548	37.0	33.0	40.0	26.5	41.0
56-57	34.939520036508554	37.0	33.0	40.0	26.5	41.0
58-59	34.65355629716157	36.5	33.0	40.0	26.0	41.0
60-61	34.39260861481601	36.0	33.0	39.5	26.0	41.0
62-63	34.111415025597296	35.5	33.0	39.0	26.0	41.0
64-65	33.821922032459554	35.0	32.5	39.0	26.0	41.0
66-67	33.53422911369108	35.0	32.0	38.0	25.5	40.0
68-69	33.24770582070997	35.0	32.0	37.5	25.0	40.0
70-71	33.200463587228015	35.0	32.0	37.0	25.0	40.0
72-73	33.00285110370757	35.0	32.0	37.0	25.0	40.0
74-75	32.75395086745368	35.0	32.0	36.0	25.0	39.0
76-77	32.5368952332303	35.0	32.0	36.0	25.0	39.0
78-79	32.33130345291261	35.0	31.0	35.5	24.0	39.0
80-81	32.136337369806824	35.0	31.0	35.0	24.0	37.5
82-83	31.902118115678018	35.0	31.0	35.0	24.0	37.0
84-85	31.736766401616592	35.0	31.0	35.0	24.0	37.0
86-87	31.579927245820485	34.5	31.0	35.0	23.5	36.0
88-89	31.606731827418223	35.0	31.0	35.0	24.0	36.0
90-91	31.536620949597918	35.0	31.0	35.0	24.0	36.0
92-93	31.39251058259684	35.0	31.0	35.0	23.0	36.0
94-95	31.38312437321354	35.0	31.0	35.0	23.0	35.0
96-97	31.292602980780426	35.0	31.0	35.0	23.0	35.0
98-99	31.2155596099745	34.5	31.0	35.0	23.0	35.0
100	30.713856910520246	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.732788587188093
1101	2	0.83342009443842
1101	3	0.8825522261572765
1101	4	0.9453337776260256
1101	5	0.8357838783649427
1101	6	0.5494391837100636
1101	7	0.6333812061298261
1101	8	0.6545782694659366
1101	9	0.7721988396271726
1101	10-11	0.8939039217314217
1101	12-13	0.7388511179510004
1101	14-15	1.0189120199033326
1101	16-17	1.1033873988358494
1101	18-19	1.2090915107596203
1101	20-21	0.8896262938985586
1101	22-23	1.2061654985003472
1101	24-25	1.1147022847932746
1101	26-27	0.9812509299185095
1101	28-29	1.1659418808479103
1101	30-31	1.2267048562806622
1101	32-33	1.0580640587116648
1101	34-35	0.9979500764353446
1101	36-37	0.8975773342148372
1101	38-39	1.0430345825368974
1101	40-41	0.9129173301600488
1101	42-43	0.9674557879050951
1101	44-45	0.944864496456141
1101	46-47	0.9849632830538155
1101	48-49	0.9266526344773993
1101	50-51	0.8630285528981432
1101	52-53	0.8977534366844466
1101	54-55	0.9429839880634532
1101	56-57	0.8324665852845143
1101	58-59	0.7850758970395546
1101	60-61	0.7831715876615917
1101	62-63	0.7482647107996456
1101	64-65	0.6939381922672823
1101	66-67	0.7070709852410104
1101	68-69	0.724266809467764
1101	70-71	0.6133781949593171
1101	72-73	0.6454527137163737
1101	74-75	0.6370903863566326
1101	76-77	0.6521580536274598
1101	78-79	0.6944782152950069
1101	80-81	0.7497211126935994
1101	82-83	0.7099179424513125
1101	84-85	0.7343111745798581
1101	86-87	0.731899564416139
1101	88-89	0.6991570193488066
1101	90-91	0.7375212677528076
1101	92-93	0.8004101787320934
1101	94-95	0.783713961506951
1101	96-97	0.7353248947258528
1101	98-99	0.7675242407214142
1101	100	0.836103474753731
1102	1	0.6980894698937803
1102	2	0.8857403981450105
1102	3	0.8145915588414923
1102	4	0.8709038500570259
1102	5	0.7822585435034028
1102	6	0.609066981422238
1102	7	0.6524367623856691
1102	8	0.6341487870774714
1102	9	0.8796429929894032
1102	10-11	0.787748032604739
1102	12-13	0.7391021315207027
1102	14-15	0.9425153735762066
1102	16-17	1.1003311560550415
1102	18-19	1.1450593695242475
1102	20-21	0.9151159463718201
1102	22-23	1.2359746088986654
1102	24-25	1.0834340629052122
1102	26-27	0.9246540799397422
1102	28-29	1.1223866992139406
1102	30-31	1.1430213993185205
1102	32-33	1.038734845552895
1102	34-35	0.9555937155648593
1102	36-37	0.9088953037980687
1102	38-39	0.9399158515350905
1102	40-41	0.9348678972887043
1102	42-43	0.9338006082080312
1102	44-45	0.9545647205528951
1102	46-47	0.9264695274179644
1102	48-49	0.8948802871369637
1102	50-51	0.9671979161147348
1102	52-53	0.9400419674749259
1102	54-55	0.9371552820353983
1102	56-57	0.8280507586860182
1102	58-59	0.7780424442432974
1102	60-61	0.8108212496410516
1102	62-63	0.8010446219049641
1102	64-65	0.7549884078939186
1102	66-67	0.8207927836842899
1102	68-69	0.8330849350793912
1102	70-71	0.769497897795155
1102	72-73	0.697127551761973
1102	74-75	0.7088264874544308
1102	76-77	0.7260399986974733
1102	78-79	0.7551738083458872
1102	80-81	0.7832429688135889
1102	82-83	0.7500755172995923
1102	84-85	0.7357531690624484
1102	86-87	0.7275963319073924
1102	88-89	0.6782835785183146
1102	90-91	0.7169171642212753
1102	92-93	0.7890027272461566
1102	94-95	0.7640820763118761
1102	96-97	0.7549524756892119
1102	98-99	0.7706536861857245
1102	100	0.8021061778112433
1103	1	0.5768671687075759
1103	2	0.7481108009318156
1103	3	0.7268024809395861
1103	4	0.6974702099899801
1103	5	0.6957967807146801
1103	6	0.5819472811135427
1103	7	0.5609157321722904
1103	8	0.5853966683041989
1103	9	0.675429028377323
1103	10-11	0.7848571156796567
1103	12-13	0.6312736492626314
1103	14-15	0.7877566111022247
1103	16-17	1.0205055934213334
1103	18-19	1.0401577363794772
1103	20-21	0.7412423630117502
1103	22-23	0.9545032984142168
1103	24-25	0.8916911648370913
1103	26-27	0.8212302250733003
1103	28-29	0.9934136028461111
1103	30-31	1.0206880963245197
1103	32-33	0.8060922836384421
1103	34-35	0.7701518906203546
1103	36-37	0.7523968104983041
1103	38-39	0.8018123708169611
1103	40-41	0.7822408635957956
1103	42-43	0.7723473802716967
1103	44-45	0.7788458929136937
1103	46-47	0.7552424148427974
1103	48-49	0.7060121403399577
1103	50-51	0.7235691374505748
1103	52-53	0.8328474163575947
1103	54-55	0.8461912773539453
1103	56-57	0.7398551873703525
1103	58-59	0.7641582773598117
1103	60-61	0.7339079640477308
1103	62-63	0.677222033138662
1103	64-65	0.6599560980380232
1103	66-67	0.7150813585355564
1103	68-69	0.6998141344795883
1103	70-71	0.6438390286431783
1103	72-73	0.6224758641443131
1103	74-75	0.635063627331931
1103	76-77	0.6965942599791859
1103	78-79	0.6203164215920438
1103	80-81	0.6643321339640593
1103	82-83	0.615220451964305
1103	84-85	0.5329887502950896
1103	86-87	0.558707309198553
1103	88-89	0.5969332805952696
1103	90-91	0.601492165347512
1103	92-93	0.6518539854550198
1103	94-95	0.5981913533746344
1103	96-97	0.5623017114291962
1103	98-99	0.603223134907811
1103	100	0.6688639084414945
1104	1	0.5498181752650488
1104	2	0.70543972890734
1104	3	0.6910841396531886
1104	4	0.7578950521867824
1104	5	0.7294807789427864
1104	6	0.3976282631801027
1104	7	0.5285866354612878
1104	8	0.5116952447332608
1104	9	0.6327304554295452
1104	10-11	0.5886251728924634
1104	12-13	0.5671019542785913
1104	14-15	0.7610637309343744
1104	16-17	0.9108853885729076
1104	18-19	0.8496121127325438
1104	20-21	0.687429598296859
1104	22-23	0.9731306521409877
1104	24-25	0.8133694825407787
1104	26-27	0.683106119889402
1104	28-29	0.855707532418414
1104	30-31	0.8934431225400914
1104	32-33	0.6886054826014956
1104	34-35	0.6254896185361076
1104	36-37	0.6493713951455575
1104	38-39	0.7005009527296338
1104	40-41	0.6006070266373698
1104	42-43	0.6046113302852589
1104	44-45	0.660390305987157
1104	46-47	0.6242521572150963
1104	48-49	0.6030639658743411
1104	50-51	0.6612811521036832
1104	52-53	0.6888952764305287
1104	54-55	0.6657882695285338
1104	56-57	0.5982645079157862
1104	58-59	0.6431314281106921
1104	60-61	0.6629571360538336
1104	62-63	0.6009202847785673
1104	64-65	0.5712024925752104
1104	66-67	0.638462394869812
1104	68-69	0.6045484791127578
1104	70-71	0.5184578196658762
1104	72-73	0.5053871234308289
1104	74-75	0.5729650253616398
1104	76-77	0.5215431938136774
1104	78-79	0.5232624365395182
1104	80-81	0.5644511117206932
1104	82-83	0.5478807187013501
1104	84-85	0.5200697401294008
1104	86-87	0.5230314256077975
1104	88-89	0.3978735901520558
1104	90-91	0.47742536137160485
1104	92-93	0.47646186639668286
1104	94-95	0.38428277029206726
1104	96-97	0.4448261520237047
1104	98-99	0.5318898250198316
1104	100	0.6281148519688671
1105	1	0.5051564028639888
1105	2	0.6169666812617542
1105	3	0.5618097709252012
1105	4	0.6666047402043347
1105	5	0.5438189763252694
1105	6	0.31818723633877966
1105	7	0.3834844822753922
1105	8	0.44699013330890125
1105	9	0.4615092983505207
1105	10-11	0.5587166124281495
1105	12-13	0.47824055929539355
1105	14-15	0.6894487347977645
1105	16-17	0.7696751022216688
1105	18-19	0.7942940054526346
1105	20-21	0.6066210972102652
1105	22-23	0.8525808338473695
1105	24-25	0.7019602304554908
1105	26-27	0.5986585449514052
1105	28-29	0.7704417751390054
1105	30-31	0.8074367025036153
1105	32-33	0.666123664031609
1105	34-35	0.6058628948195022
1105	36-37	0.5287703467786073
1105	38-39	0.6416546551800835
1105	40-41	0.4834937300562032
1105	42-43	0.5525317790043403
1105	44-45	0.6466959288369338
1105	46-47	0.522925726981768
1105	48-49	0.6137531851244233
1105	50-51	0.6267840677513448
1105	52-53	0.5932252278395964
1105	54-55	0.5206540413406131
1105	56-57	0.5289811246134022
1105	58-59	0.5340501317098614
1105	60-61	0.5533887492717611
1105	62-63	0.5172907722754303
1105	64-65	0.4593135543381095
1105	66-67	0.5821599747239787
1105	68-69	0.5012298015572938
1105	70-71	0.4495048988142756
1105	72-73	0.4287470836122651
1105	74-75	0.45976278066306975
1105	76-77	0.45556698303331444
1105	78-79	0.5064977023027879
1105	80-81	0.5498764406363534
1105	82-83	0.5011857808681057
1105	84-85	0.4517856580116444
1105	86-87	0.5287490794970608
1105	88-89	0.5398558348698153
1105	90-91	0.5563661380878706
1105	92-93	0.5538516219168201
1105	94-95	0.4689375437478276
1105	96-97	0.5253264033478366
1105	98-99	0.5306074844247952
1105	100	0.590954013639827
1106	1	0.5118497303981542
1106	2	0.5864571055748193
1106	3	0.5812887236966588
1106	4	0.6557836314194248
1106	5	0.5659951768119313
1106	6	0.31705999887978464
1106	7	0.3173490711934761
1106	8	0.39397935005978724
1106	9	0.4831683225063088
1106	10-11	0.45922308876531304
1106	12-13	0.41076441926458784
1106	14-15	0.6745932080386936
1106	16-17	0.7563596807615482
1106	18-19	0.7231672476691529
1106	20-21	0.5687287950094273
1106	22-23	0.8323763270028408
1106	24-25	0.7063875098531938
1106	26-27	0.5544378454098577
1106	28-29	0.7607589088373672
1106	30-31	0.7866389710219011
1106	32-33	0.6420819624060456
1106	34-35	0.6064337804536137
1106	36-37	0.5203134042949316
1106	38-39	0.6655415687468675
1106	40-41	0.5777740720445053
1106	42-43	0.5022303549781242
1106	44-45	0.5058905907302886
1106	46-47	0.4906878540998463
1106	48-49	0.4983879941597422
1106	50-51	0.5295345223665038
1106	52-53	0.5110392879064563
1106	54-55	0.5176650408623757
1106	56-57	0.5365591005932586
1106	58-59	0.5219086592519631
1106	60-61	0.5083695275614275
1106	62-63	0.4675325418929077
1106	64-65	0.47027434917838207
1106	66-67	0.529501262536705
1106	68-69	0.4310272863829212
1106	70-71	0.4630242122730195
1106	72-73	0.4425673605608793
1106	74-75	0.49479177292103316
1106	76-77	0.48125109481661354
1106	78-79	0.4851286366126075
1106	80-81	0.5187904468982083
1106	82-83	0.5289440107721504
1106	84-85	0.4250160859946419
1106	86-87	0.48359435842538545
1106	88-89	0.4686050911335222
1106	90-91	0.43586306613103076
1106	92-93	0.5245537950981038
1106	94-95	0.49957161377183823
1106	96-97	0.5258220182045221
1106	98-99	0.5459639192179928
1106	100	0.5055997631047084
1107	1	0.47727005910838116
1107	2	0.5962621176215492
1107	3	0.539733002272115
1107	4	0.612250218908656
1107	5	0.5335159408235697
1107	6	0.3474314279347581
1107	7	0.2984742038174204
1107	8	0.41421661715765623
1107	9	0.42734591968928726
1107	10-11	0.4287419358036928
1107	12-13	0.37957589496760846
1107	14-15	0.5875836985462115
1107	16-17	0.7087378477498589
1107	18-19	0.7521918708391695
1107	20-21	0.4633564848481484
1107	22-23	0.7177711132263838
1107	24-25	0.4846327901331122
1107	26-27	0.39082200932983113
1107	28-29	0.5974513291072796
1107	30-31	0.6046555797885418
1107	32-33	0.5205485050548191
1107	34-35	0.37443979849692965
1107	36-37	0.35360564841337094
1107	38-39	0.413689173594598
1107	40-41	0.23892001884208725
1107	42-43	0.42423039737591495
1107	44-45	0.33150796272691707
1107	46-47	0.3603943898672668
1107	48-49	0.4134019758337786
1107	50-51	0.46971412149279246
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2214	96-97	-1.2180583352475374
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2214	100	-1.2396563077722327
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2215	4	-1.9238385729619196
2215	5	-1.454719686203937
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2215	9	-1.6942244897571328
2215	10-11	-1.3305439280451665
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2215	14-15	-3.6386444468554586
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2215	22-23	-3.0656767871334196
2215	24-25	-5.297955894722346
2215	26-27	-3.438126776385552
2215	28-29	-1.6526999247787302
2215	30-31	-3.8157097587867312
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2215	100	-3.035808841729626
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2216	3	-0.7897654998290591
2216	4	-0.7845270447577661
2216	5	-0.9256978672169254
2216	6	-0.6794906689286009
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2216	9	-0.9763277329992022
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2216	12-13	-1.1585042812872288
2216	14-15	-1.5466666697647398
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2216	32-33	-1.2266613130824808
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2216	36-37	-1.435283479870776
2216	38-39	-1.563341098497915
2216	40-41	-1.548195070189081
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2216	62-63	-1.439672237162867
2216	64-65	-1.4148284132694684
2216	66-67	-1.3406964757711464
2216	68-69	-1.5153603009282435
2216	70-71	-1.5411257747651135
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2216	74-75	-1.4885629163325724
2216	76-77	-1.4024716191887237
2216	78-79	-1.5408878760026923
2216	80-81	-1.5731571838411256
2216	82-83	-1.727144477684682
2216	84-85	-1.5063601709239407
2216	86-87	-1.5270448462791961
2216	88-89	-1.3810024277597996
2216	90-91	-1.5009929329576117
2216	92-93	-1.4810168103197512
2216	94-95	-1.364770681474429
2216	96-97	-1.34951224494365
2216	98-99	-1.295303745962201
2216	100	-1.2628476259900623
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	22225.0
1	3666.0
2	552.0
3	557.0
4	532.0
5	291.0
6	288.0
7	385.0
8	676.0
9	1195.0
10	1980.0
11	3675.0
12	6348.0
13	8827.0
14	11623.0
15	14901.0
16	18018.0
17	21059.0
18	24360.0
19	27675.0
20	31966.0
21	36068.0
22	41874.0
23	48262.0
24	54638.0
25	61870.0
26	70986.0
27	82427.0
28	96775.0
29	115697.0
30	137787.0
31	166567.0
32	204355.0
33	261017.0
34	361067.0
35	485869.0
36	721965.0
37	1085002.0
38	811927.0
39	279698.0
40	130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.433408141081216	21.358206435236916	12.288756642112272	23.919628781569596
2	15.403746370293456	19.675424245534177	40.97274626162864	23.94808312254372
3	18.355582655964646	27.2240594261394	28.765121547033267	25.65523637086268
4	12.088149094688442	16.74313138395792	40.56002590378314	30.60869361757049
5	12.982693878752086	38.728838448724765	35.14919307453344	13.139274597989708
6	29.29768226806544	38.83723107224591	18.44969804901046	13.415388610678184
7	26.67585693459702	31.678336343797316	24.20537625011019	17.44043047149548
8	26.2536852553166	34.830207371001435	19.993121234281624	18.92298613940034
9	26.258488015159532	14.760910168588826	21.94817069314236	37.03243112310928
10-11	25.786918984516387	25.64466117329462	27.52690535352171	21.041514488667286
12-13	25.941738645257345	23.565014864613534	27.53929044482439	22.953956045304736
14-15	23.464577053706904	24.60108401677571	24.922699702505255	27.01163922701213
16-17	22.224260440054902	27.199193507729667	26.349593402292022	24.226952649923405
18-19	21.543187638049126	28.15646996047296	27.96257336972222	22.337769031755688
20-21	23.43285939476229	27.167704165506628	26.670144664506797	22.72929177522429
22-23	24.07144055002704	26.737499078686017	26.448067288526474	22.742993082760467
24-25	23.422100542836912	26.819258909605853	27.271172999763984	22.48746754779325
26-27	23.287599085547864	27.11932380906446	26.092485916857573	23.5005911885301
28-29	22.313852064843044	27.658188908251912	26.7494167022054	23.27854232469964
30-31	23.23609217308483	27.19654438247403	26.692162795757696	22.875200648683446
32-33	23.745566861219746	27.492417527335135	25.54776462192418	23.21425098952094
34-35	23.66440500413134	27.213504493114183	26.758575737938685	22.363514764815797
36-37	23.286617797266114	26.772554459675096	26.36780545759611	23.57302228546268
38-39	23.51074178411025	26.807691185591477	27.071424100144675	22.610142930153597
40-41	22.97463592172278	25.87727245302416	27.759251955660524	23.38883966959254
42-43	23.03084522342097	26.179619595877863	26.997220781023323	23.792314399677846
44-45	23.127230162390383	27.29297615230014	26.743950634351005	22.835843050958466
46-47	23.098816311865537	26.93900637428643	26.297545703789492	23.66463161005854
48-49	23.941970884434348	26.43840398503422	26.55897150955034	23.06065362098109
50-51	23.405058847677335	26.29815829212293	26.492709021183046	23.804073839016684
52-53	22.780648581859708	27.016018180472457	26.3485814065547	23.854751831113138
54-55	22.77915090155033	26.896851055505106	26.343554246068184	23.980443796876376
56-57	24.09521162597102	26.215304165190023	26.02843959112211	23.661044617716843
58-59	22.787308297333077	26.64067800258571	27.142318194374766	23.429695505706448
60-61	22.829074124405384	27.426312260255177	26.26637047730561	23.47824313803383
62-63	23.384787131821014	26.558502960384622	25.755605832204566	24.301104075589798
64-65	22.40401850494109	26.775037626751313	26.365320965487992	24.455622902819606
66-67	23.24874351056497	26.282444342925203	26.306414338796774	24.162397807713052
68-69	22.529626353699083	26.400601162457676	26.64908680699393	24.420685676849306
70-71	22.551753939496592	26.03452884133431	26.788727875898367	24.62498934327074
72-73	23.241284504148616	26.16305747638668	27.264962026081342	23.33069599338336
74-75	23.392449128860022	26.523462323865104	26.710701314439994	23.373387232834876
76-77	22.979965111485505	26.424580515126205	26.366099442744765	24.229354930643527
78-79	22.814838037698504	26.413703362005553	26.444648204264286	24.326810396031657
80-81	22.363900494536534	26.808290812858555	27.239603630629244	23.58820506197567
82-83	23.32217904548423	25.540011845963207	28.38966081914628	22.748148289406284
84-85	23.599243139571563	26.260622873737603	26.760308994378494	23.37982499231234
86-87	21.97943120931473	27.03752136544919	27.386594544385584	23.596452880850496
88-89	23.240472292342726	25.564548009763094	27.153153722916873	24.041825974977307
90-91	22.843254371611344	25.607780286513698	27.345571816287357	24.2033935255876
92-93	23.151117585971384	25.894632315302612	27.21405231628441	23.740197782441594
94-95	21.80983775299314	25.92154809083326	28.05111305349408	24.217501102679524
96-97	22.24211731375246	25.80540226745175	27.39300588468702	24.559474534108766
98-99	21.497538100130523	26.551632105882156	27.3746517028121	24.576178091175223
100	22.412625980008016	25.7695800802482	26.441859551030106	25.375934388713677
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18492.0
1	10853.0
2	2942.0
3	2042.5
4	1009.0
5	491.0
6	354.0
7	323.5
8	312.5
9	330.5
10	329.5
11	301.0
12	295.0
13	310.5
14	318.5
15	372.5
16	444.5
17	513.0
18	635.5
19	760.0
20	941.5
21	1176.5
22	1567.5
23	2264.0
24	3121.0
25	4239.5
26	6188.5
27	8421.5
28	12754.0
29	23565.0
30	38578.0
31	52448.5
32	71636.0
33	89046.0
34	102901.0
35	119168.0
36	132976.0
37	158942.0
38	170144.0
39	157850.0
40	144730.0
41	131682.5
42	130435.0
43	140885.5
44	156397.5
45	189562.0
46	223586.5
47	246871.0
48	261989.0
49	256630.0
50	244145.5
51	250706.0
52	253611.5
53	242198.5
54	229336.5
55	197294.5
56	172029.5
57	142623.0
58	111401.0
59	106239.5
60	89002.5
61	61517.5
62	45737.5
63	38229.0
64	27803.5
65	13378.0
66	7114.5
67	4813.5
68	3505.0
69	2468.5
70	1806.0
71	1442.0
72	1150.0
73	1002.5
74	864.0
75	670.5
76	488.0
77	321.5
78	230.5
79	185.0
80	138.5
81	89.5
82	60.5
83	49.0
84	35.5
85	32.0
86	31.5
87	26.0
88	21.5
89	16.5
90	12.0
91	11.5
92	12.0
93	10.0
94	4.5
95	2.5
96	4.5
97	4.0
98	4.0
99	4.0
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0045485447286084
2	0.45215013578025753
3	0.48766334008165596
4	0.47265802530808787
5	0.4387223509703687
6	0.49275275222638304
7	0.5098238800476264
8	0.5130352803308306
9	0.5267259868013326
10-11	0.5401443815519139
12-13	0.5379564977332397
14-15	0.5362099467020234
16-17	0.5400974312553758
18-19	0.5382006392752376
20-21	0.5376654058947037
22-23	0.5003117499690128
24-25	0.5357967840924883
26-27	0.514584640116587
28-29	0.521016830742303
30-31	0.5239183590683559
32-33	0.5263128241917976
34-35	0.5385386814103118
36-37	0.5567647865263917
38-39	0.5461540195087872
40-41	0.5506049076205966
42-43	0.5488959168266107
44-45	0.5589338902264507
46-47	0.5573469702034638
48-49	0.5289044805606993
50-51	0.5444168585368785
52-53	0.5361348262275624
54-55	0.5459192680260969
56-57	0.5339375523495806
58-59	0.5399847505436844
60-61	0.5332332979015095
62-63	0.5274678014866342
64-65	0.5386325820033879
66-67	0.5366512794894812
68-69	0.5578728135246902
70-71	0.5414402097363648
72-73	0.5453746445862553
74-75	0.5422665349554348
76-77	0.5294960542970789
78-79	0.5610560436299715
80-81	0.5353836214829533
82-83	0.5345009559080375
84-85	0.5437877245632683
86-87	0.8493872047295851
88-89	1.1162808604299146
90-91	1.1072476233759894
92-93	1.1069659215967607
94-95	1.111736071725029
96-97	1.1047310874815486
98-99	1.1009562836398874
100	1.0961767434523115
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5324780.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.369452363045742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.92651616098496	17.042527454270235
2	12.304948060110144	5.751197950409923
3	5.121774728849374	3.5907921162029095
4	2.7341254442992344	2.5558005730056896
5	1.628853949348159	1.9032712387825361
6	1.0526322931153858	1.4759664137858166
7	0.6958627174562445	1.1383351438769282
8	0.4936808141061874	0.922964021424335
9	0.38544018538893926	0.8106773446125303
>10	2.051017960998984	8.84299443363382
>50	0.21883775672425732	3.636752836213128
>100	0.27516760661505324	14.512609233883483
>500	0.05721860684512454	9.361750105764573
>1k	0.05030737062432089	21.263141891473037
>5k	0.002893075626957751	4.5086208341011025
>10k+	7.232689067394378E-4	2.6825984085600094
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	22430	0.42123806053959034	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	18770	0.3525028264078516	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	16614	0.3120128906734175	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	16609	0.31191899008034135	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	15448	0.2901152723680603	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	15084	0.28327930919211686	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	14225	0.267147187301635	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	13357	0.25084604434361607	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	10303	0.19349156209270618	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	9385	0.1762514132039258	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	9244	0.17360341647917849	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	8754	0.1644011583577162	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	8345	0.15672008984408745	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	8265	0.15521768035486913	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	7920	0.14873853943261506	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	7771	0.1459403017589459	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	7741	0.14537689820048905	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	7330	0.13765826944962983	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	7172	0.13469101070842365	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	7045	0.13230593564428952	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6951	0.13054060449445798	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	6929	0.13012744188492295	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	6769	0.1271226229064863	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	6682	0.12548875258696135	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	6666	0.12518827068911767	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	6605	0.1240426834535887	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	6581	0.12359196060682319	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	6305	0.11840864786901995	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	6286	0.11805182561533058	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	6231	0.11701891909149298	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	6197	0.1163803950585752	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	6108	0.11470896450181979	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	6021	0.11307509418229486	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	5925	0.11127220279523287	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5824	0.10937541081509472	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	5817	0.10924394998478812	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	5736	0.10772276037695455	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	5629	0.10571328768512502	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5612	0.10539402566866612	No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA	5552	0.10426721855175237	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	5474	0.10280236929976451	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	5425	0.10188214348761826	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	5335	0.10019193281224764	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8780118615229174E-5
2	0.0	0.0	0.0	0.0	1.8780118615229174E-5
3	0.0	0.0	0.0	0.0	1.8780118615229174E-5
4	0.0	0.0	0.0	0.0	1.8780118615229174E-5
5	0.0	0.0	0.0	0.0	1.8780118615229174E-5
6	0.0	0.0	0.0	0.0	1.8780118615229174E-5
7	0.0	0.0	0.0	0.0	1.8780118615229174E-5
8	0.0	0.0	0.0	0.0	1.8780118615229174E-5
9	0.0	0.0	0.0	0.0	1.8780118615229174E-5
10-11	0.0	0.0	0.0	0.0	1.8780118615229174E-5
12-13	0.0	0.0	0.0	0.0	1.8780118615229174E-5
14-15	0.0	0.0	0.0	1.8780118615229174E-5	2.817017792284376E-5
16-17	0.0	0.0	0.0	1.8780118615229174E-5	3.756023723045835E-5
18-19	0.0	0.0	0.0	2.817017792284376E-5	3.756023723045835E-5
20-21	0.0	0.0	0.0	6.573041515330211E-5	3.756023723045835E-5
22-23	0.0	0.0	0.0	9.390059307614587E-5	3.756023723045835E-5
24-25	0.0	0.0	0.0	2.253614233827501E-4	3.756023723045835E-5
26-27	0.0	0.0	0.0	5.352333805340315E-4	3.756023723045835E-5
28-29	0.0	0.0	0.0	0.0018686218022153029	3.756023723045835E-5
30-31	0.0	0.0	0.0	0.008328982605854138	3.756023723045835E-5
32-33	0.0	0.0	0.0	0.021916398423972444	3.756023723045835E-5
34-35	0.0	0.0	0.0	0.04072468721712447	3.756023723045835E-5
36-37	0.0	0.0	0.0	0.0756369277228355	3.756023723045835E-5
38-39	0.0	0.0	0.0	0.1313950998914509	3.756023723045835E-5
40-41	0.0	0.0	0.0	0.19329437084724627	3.756023723045835E-5
42-43	0.0	0.0	0.0	0.2601515931174621	3.756023723045835E-5
44-45	0.0	0.0	0.0	0.3388121199373495	3.756023723045835E-5
46-47	0.0	0.0	0.0	0.43878808138552206	3.756023723045835E-5
48-49	0.0	0.0	0.0	0.5592907124801401	3.756023723045835E-5
50-51	0.0	0.0	0.0	0.693192958206724	4.6950296538072935E-5
52-53	0.0	0.0	0.0	0.8269449629843862	5.6340355845687524E-5
54-55	0.0	0.0	0.0	0.9745754754187028	5.6340355845687524E-5
56-57	0.0	0.0	0.0	1.1537002467707587	5.6340355845687524E-5
58-59	0.0	0.0	0.0	1.372498018697486	5.6340355845687524E-5
60-61	0.0	0.0	0.0	1.6037470092661106	5.6340355845687524E-5
62-63	0.0	0.0	0.0	1.8469683254519436	5.6340355845687524E-5
64-65	0.0	0.0	0.0	2.100678337884382	5.6340355845687524E-5
66-67	0.0	0.0	0.0	2.393629408163342	5.6340355845687524E-5
68-69	0.0	0.0	0.0	2.72433790691822	5.6340355845687524E-5
70-71	0.0	0.0	0.0	3.0951231787979974	5.6340355845687524E-5
72-73	0.0	0.0	0.0	3.4818903316193346	5.6340355845687524E-5
74-75	0.0	0.0	0.0	3.8575584343390714	5.6340355845687524E-5
76-77	0.0	0.0	0.0	4.29305060490762	5.6340355845687524E-5
78-79	0.0	9.390059307614587E-6	0.0	4.765671069978478	5.6340355845687524E-5
80-81	0.0	1.8780118615229174E-5	0.0	5.276030183406638	5.6340355845687524E-5
82-83	0.0	1.8780118615229174E-5	0.0	5.811357464533746	5.6340355845687524E-5
84-85	0.0	1.8780118615229174E-5	0.0	6.332045643200283	5.6340355845687524E-5
86-87	0.0	2.817017792284376E-5	0.0	6.884171740428712	5.6340355845687524E-5
88	0.0	3.756023723045835E-5	0.0	7.330781741217478	5.6340355845687524E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5750	0.0	40.98637	94
TACCCCG	345	0.0	34.047775	5
GTACTAG	6310	0.0	33.358223	1
CAACGCA	9720	0.0	31.662329	5
GTATCAA	11360	0.0	30.992592	1
CAGTACG	880	0.0	30.945475	9
TAGGCAT	7575	0.0	30.64165	5
GCATCGT	6670	0.0	29.280212	8
ATCAACG	10550	0.0	29.174946	3
CTGTGCG	8665	0.0	28.989254	9
TAGTACT	8815	0.0	28.46528	4
TATTCTC	2760	0.0	28.089415	5
CTAACGC	3075	0.0	27.965796	3
CTAGGCA	8660	0.0	27.78105	4
ACTGTGC	9465	0.0	27.577883	8
TCTAACG	3160	0.0	27.511747	2
GGTATCA	7740	0.0	27.256115	1
AGGGCTA	6335	0.0	26.923271	5
CGCCTAC	3190	0.0	26.196064	7
GTACATG	22940	0.0	26.169237	1
>>END_MODULE