##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146478_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3510338
Sequences flagged as poor quality	0
Sequence length	100
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.60319604550901	31.0	31.0	34.0	27.0	34.0
2	30.65277360755574	31.0	31.0	34.0	27.0	34.0
3	30.816237068909032	31.0	31.0	34.0	27.0	34.0
4	34.270873915845144	37.0	35.0	37.0	30.0	37.0
5	34.46101913832799	37.0	35.0	37.0	30.0	37.0
6	34.62460794373647	37.0	35.0	37.0	31.0	37.0
7	34.46764328677182	37.0	35.0	37.0	30.0	37.0
8	34.4295905408539	37.0	35.0	37.0	30.0	37.0
9	35.78326930341181	39.0	35.0	39.0	30.0	39.0
10-11	35.70765735379328	39.0	35.0	39.0	29.5	39.0
12-13	35.706123028608644	39.0	35.0	39.0	30.0	39.0
14-15	36.72429022504386	40.0	36.0	41.0	29.5	41.0
16-17	36.57832849714187	40.0	36.0	41.0	28.0	41.0
18-19	36.543095137847125	40.0	36.0	41.0	28.0	41.0
20-21	36.88022463933673	40.0	36.0	41.0	30.0	41.0
22-23	36.605325185210084	40.0	36.0	41.0	28.5	41.0
24-25	36.73008653297774	40.0	36.0	41.0	29.5	41.0
26-27	36.80131756543102	40.0	36.0	41.0	30.0	41.0
28-29	36.65311744908895	40.0	35.5	41.0	29.5	41.0
30-31	36.6045912103051	40.0	35.0	41.0	29.5	41.0
32-33	36.705507845683236	40.0	35.0	41.0	30.0	41.0
34-35	36.748966908599684	40.0	35.0	41.0	30.0	41.0
36-37	36.685651353231506	40.0	35.0	41.0	30.0	41.0
38-39	36.51476780868395	39.0	35.0	41.0	29.0	41.0
40-41	36.475900753716594	39.0	35.0	41.0	29.0	41.0
42-43	36.313833311777955	39.0	35.0	41.0	29.0	41.0
44-45	36.12225318473605	39.0	35.0	41.0	28.0	41.0
46-47	35.990218320856854	39.0	34.5	41.0	28.0	41.0
48-49	35.8549652483607	38.0	34.0	40.5	28.0	41.0
50-51	35.63395846781705	38.0	34.0	40.0	27.5	41.0
52-53	35.4435591387496	38.0	34.0	40.0	27.0	41.0
54-55	35.25087455965779	38.0	34.0	40.0	27.0	41.0
56-57	35.09702555708311	37.0	33.0	40.0	27.0	41.0
58-59	34.82705397599889	37.0	33.0	40.0	26.5	41.0
60-61	34.57706893182366	36.0	33.0	40.0	26.0	41.0
62-63	34.307160592512744	36.0	33.0	39.0	26.0	41.0
64-65	34.03172913833369	35.0	33.0	39.0	26.0	41.0
66-67	33.752199218422845	35.0	32.0	39.0	26.0	41.0
68-69	33.4769932411067	35.0	32.0	38.0	25.0	40.0
70-71	33.425735498974745	35.0	32.0	37.5	26.0	40.0
72-73	33.22841646587878	35.0	32.0	37.0	25.0	40.0
74-75	32.973777881218275	35.0	32.0	36.5	25.0	39.5
76-77	32.74503637541456	35.0	32.0	36.0	25.0	39.0
78-79	32.53092138135986	35.0	32.0	36.0	25.0	39.0
80-81	32.315625019585006	35.0	31.5	36.0	24.5	38.0
82-83	32.075144473267244	35.0	31.0	35.0	24.0	37.0
84-85	31.887767930039786	35.0	31.0	35.0	24.0	37.0
86-87	31.71652516082497	35.0	31.0	35.0	24.0	36.5
88-89	31.7157977379956	35.0	31.5	35.0	24.0	36.0
90-91	31.636554371687286	35.0	31.5	35.0	24.0	36.0
92-93	31.484922534525165	35.0	31.0	35.0	23.5	36.0
94-95	31.463061819118273	35.0	31.0	35.0	24.0	35.5
96-97	31.37072028391568	35.0	31.0	35.0	23.0	35.0
98-99	31.29315239729052	35.0	31.0	35.0	23.0	35.0
100	30.812073082421122	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7249495066337808
1101	2	0.8471169174371163
1101	3	0.8258664886224025
1101	4	0.9468924093322997
1101	5	0.8287495977799821
1101	6	0.5348918653941297
1101	7	0.6032750483718488
1101	8	0.6522555901014329
1101	9	0.8227302381912196
1101	10-11	0.8889744830647004
1101	12-13	0.7225172822154491
1101	14-15	1.0180015799798738
1101	16-17	1.0962256442757266
1101	18-19	1.263059068801283
1101	20-21	0.8643608651629364
1101	22-23	1.2084291367222022
1101	24-25	1.0425340042635582
1101	26-27	0.9705511938831961
1101	28-29	1.134153272440372
1101	30-31	1.1342600734515713
1101	32-33	1.022516487355695
1101	34-35	0.9269520955047028
1101	36-37	0.9249747364942493
1101	38-39	0.9727132685927486
1101	40-41	0.919132188503788
1101	42-43	0.9237981904318104
1101	44-45	0.944320051540771
1101	46-47	0.9067051338181429
1101	48-49	0.8323623096328348
1101	50-51	0.9192604696521798
1101	52-53	0.9972959310288303
1101	54-55	0.9810446579160725
1101	56-57	0.8147826885016727
1101	58-59	0.7913615902489184
1101	60-61	0.8948672473977695
1101	62-63	0.8621315251932629
1101	64-65	0.7926343944599452
1101	66-67	0.807627647928399
1101	68-69	0.7732582234798926
1101	70-71	0.7524214135428053
1101	72-73	0.6756093648175536
1101	74-75	0.6991544520650024
1101	76-77	0.7298158585969219
1101	78-79	0.6960254132991679
1101	80-81	0.6974831778159754
1101	82-83	0.7064981285790708
1101	84-85	0.7251624734668844
1101	86-87	0.7290666045900025
1101	88-89	0.7039343163670679
1101	90-91	0.6772672986394355
1101	92-93	0.7532821437724273
1101	94-95	0.7326890599893119
1101	96-97	0.7787347719318412
1101	98-99	0.7646765667595865
1101	100	0.7724871517087593
1102	1	0.8810626381928905
1102	2	0.9310439197111293
1102	3	0.9504528861161461
1102	4	1.0826035047770333
1102	5	0.9182582298805784
1102	6	0.752860474473529
1102	7	0.8113153963424793
1102	8	0.8032845801676274
1102	9	1.0183717629576492
1102	10-11	0.9649082659832189
1102	12-13	0.9827874429584256
1102	14-15	1.1440503382153793
1102	16-17	1.4206391532733846
1102	18-19	1.436536601948447
1102	20-21	1.1369958957432118
1102	22-23	1.386543096581022
1102	24-25	1.2354181554463395
1102	26-27	1.1299118422735006
1102	28-29	1.3057137734204503
1102	30-31	1.3235103492101103
1102	32-33	1.1928218967432542
1102	34-35	1.0852014111757384
1102	36-37	0.9978581192680167
1102	38-39	1.051424518143044
1102	40-41	1.1213341994188681
1102	42-43	1.1241585975642892
1102	44-45	1.1479834167795673
1102	46-47	1.015385252915891
1102	48-49	1.0162073965400324
1102	50-51	1.090784686613027
1102	52-53	1.0606123214614058
1102	54-55	1.101691970469119
1102	56-57	0.9912073844892646
1102	58-59	0.9570259822459946
1102	60-61	0.9939180645174162
1102	62-63	0.9429586892427935
1102	64-65	0.8427463152098582
1102	66-67	0.9471602539663664
1102	68-69	0.9458961391252529
1102	70-71	0.8555807822778476
1102	72-73	0.8456555774157053
1102	74-75	0.9154892738612119
1102	76-77	0.8075330143027202
1102	78-79	0.7790691336530955
1102	80-81	0.8692979141210273
1102	82-83	0.8787039200947291
1102	84-85	0.8589471611658333
1102	86-87	0.9283898890921662
1102	88-89	0.8936340902090407
1102	90-91	0.9477867678496068
1102	92-93	0.8990355610384348
1102	94-95	0.9534090218005176
1102	96-97	0.9657543662203558
1102	98-99	0.8918369755305768
1102	100	0.9685823061695658
1103	1	0.4771715271745549
1103	2	0.6968735436481879
1103	3	0.662090799467979
1103	4	0.5912713209548315
1103	5	0.5759735353382496
1103	6	0.3771922834961501
1103	7	0.4290836605133279
1103	8	0.5282333376687447
1103	9	0.5602828839138141
1103	10-11	0.6748226018079251
1103	12-13	0.5535533257572567
1103	14-15	0.7225978010347589
1103	16-17	0.9487761265794106
1103	18-19	1.0189674099300987
1103	20-21	0.7108627345620917
1103	22-23	0.8728970982512081
1103	24-25	0.8199692126129321
1103	26-27	0.7532279962951307
1103	28-29	0.9153795039163768
1103	30-31	0.9658528324581184
1103	32-33	0.8165865915220749
1103	34-35	0.7637085539315791
1103	36-37	0.7201678277741976
1103	38-39	0.772219721491858
1103	40-41	0.6897670609090412
1103	42-43	0.7588661307457301
1103	44-45	0.8406058825342626
1103	46-47	0.8328109017357477
1103	48-49	0.6975234142511155
1103	50-51	0.8004658653952603
1103	52-53	0.789652136290897
1103	54-55	0.7511858423163886
1103	56-57	0.605357126081401
1103	58-59	0.6847525839229718
1103	60-61	0.6721814950504168
1103	62-63	0.5713076874673106
1103	64-65	0.6184809784330412
1103	66-67	0.6697849675029417
1103	68-69	0.6062620099688019
1103	70-71	0.5434149503107335
1103	72-73	0.5832083809749733
1103	74-75	0.5404277881220025
1103	76-77	0.5841522995748178
1103	78-79	0.5350496680143593
1103	80-81	0.6366878186024536
1103	82-83	0.570465794065079
1103	84-85	0.5320379004241893
1103	86-87	0.5774575093861287
1103	88-89	0.6484340947503071
1103	90-91	0.6295269333323432
1103	92-93	0.6790611227109515
1103	94-95	0.5367718050543075
1103	96-97	0.4838774658118261
1103	98-99	0.6083593076624929
1103	100	0.7379406611080697
1104	1	0.475687633504279
1104	2	0.6579406265977461
1104	3	0.6370633039031048
1104	4	0.774576101855935
1104	5	0.7505388739116654
1104	6	0.4345441405969339
1104	7	0.45574720404324154
1104	8	0.48364278619091294
1104	9	0.723923045823696
1104	10-11	0.6847239638155216
1104	12-13	0.5408191497606509
1104	14-15	0.7059068001775088
1104	16-17	0.8374119344264912
1104	18-19	0.7964641946022226
1104	20-21	0.6495859402928659
1104	22-23	0.9392676528897752
1104	24-25	0.7753146262198385
1104	26-27	0.7310819161991233
1104	28-29	0.8682274693172403
1104	30-31	0.9422063006226935
1104	32-33	0.7659396623968107
1104	34-35	0.6587936691548109
1104	36-37	0.6992854553631176
1104	38-39	0.7976824330142662
1104	40-41	0.7170587729431475
1104	42-43	0.6677842756454098
1104	44-45	0.6961553462275702
1104	46-47	0.5802531126037493
1104	48-49	0.5646683709245153
1104	50-51	0.6518102543050972
1104	52-53	0.7450292818714814
1104	54-55	0.6237897365873977
1104	56-57	0.4883735197463963
1104	58-59	0.5109015434966153
1104	60-61	0.5265245554736708
1104	62-63	0.47947975034007584
1104	64-65	0.4192826139065815
1104	66-67	0.5008320530803445
1104	68-69	0.5122191672365801
1104	70-71	0.4062003940342862
1104	72-73	0.40716840533212206
1104	74-75	0.42759889028071996
1104	76-77	0.4328394903805588
1104	78-79	0.37692029796878046
1104	80-81	0.44462068744935834
1104	82-83	0.4583225248613658
1104	84-85	0.49221524236236647
1104	86-87	0.4638438075129727
1104	88-89	0.409940438878472
1104	90-91	0.3891768358854293
1104	92-93	0.37066473174023074
1104	94-95	0.33463100070171947
1104	96-97	0.4018723912567417
1104	98-99	0.4582107539259148
1104	100	0.5904414157373132
1105	1	0.5857935979226774
1105	2	0.7198256711222122
1105	3	0.6421806004097732
1105	4	0.8027424583190523
1105	5	0.661472052229513
1105	6	0.3996676491405111
1105	7	0.4807239006070816
1105	8	0.5121859274529541
1105	9	0.5947697003849228
1105	10-11	0.6888262614194858
1105	12-13	0.6924158682810315
1105	14-15	0.8357690068698602
1105	16-17	0.9495910782062751
1105	18-19	0.9454215940223918
1105	20-21	0.7471550809009031
1105	22-23	1.0740930998304918
1105	24-25	0.8688448641293007
1105	26-27	0.6761423644030984
1105	28-29	0.9543753041123679
1105	30-31	0.9348957229404391
1105	32-33	0.8694275637944315
1105	34-35	0.7144440072853087
1105	36-37	0.5968442005343206
1105	38-39	0.7351606016011729
1105	40-41	0.6291320970175036
1105	42-43	0.6888605757620709
1105	44-45	0.7172673765094899
1105	46-47	0.6467008311903797
1105	48-49	0.7098074885550574
1105	50-51	0.7709638334088638
1105	52-53	0.7191673634102074
1105	54-55	0.6626725205358355
1105	56-57	0.6772635526201469
1105	58-59	0.6969726936899718
1105	60-61	0.6472102666337349
1105	62-63	0.6320181487338488
1105	64-65	0.5850924868100833
1105	66-67	0.6668416199358163
1105	68-69	0.6170322213030772
1105	70-71	0.5542915734331046
1105	72-73	0.48994404604883357
1105	74-75	0.5948374221423407
1105	76-77	0.5858635734081048
1105	78-79	0.5805006895461702
1105	80-81	0.6485298363764045
1105	82-83	0.6135231109887513
1105	84-85	0.5331558382160573
1105	86-87	0.6264069319113048
1105	88-89	0.6111278553283057
1105	90-91	0.6113704197633894
1105	92-93	0.615086494527521
1105	94-95	0.6170817906553481
1105	96-97	0.6854378196449922
1105	98-99	0.7927366582791358
1105	100	0.8015943453586125
1106	1	0.5253295657851211
1106	2	0.5241698968613804
1106	3	0.48757226144294563
1106	4	0.6028429754194349
1106	5	0.4550126189556494
1106	6	0.26427979163844384
1106	7	0.32456569652871536
1106	8	0.3318225560212298
1106	9	0.5340489567395679
1106	10-11	0.5523055146604392
1106	12-13	0.4265381893852265
1106	14-15	0.7115961641625148
1106	16-17	0.748032214267603
1106	18-19	0.7215676661249333
1106	20-21	0.5493002643944394
1106	22-23	0.8366247047716513
1106	24-25	0.6241154355756251
1106	26-27	0.5533784991296997
1106	28-29	0.7667453965473001
1106	30-31	0.7430182985081046
1106	32-33	0.553912847658772
1106	34-35	0.5156541784160211
1106	36-37	0.4039959127071384
1106	38-39	0.5892385298336151
1106	40-41	0.5923740019987491
1106	42-43	0.5955581894019559
1106	44-45	0.5790029811709445
1106	46-47	0.6004583682952713
1106	48-49	0.5377959144931026
1106	50-51	0.5091080632011895
1106	52-53	0.5208215202979432
1106	54-55	0.5242958579505057
1106	56-57	0.5128161604614547
1106	58-59	0.5766899815441278
1106	60-61	0.49304700388583456
1106	62-63	0.48652915810430386
1106	64-65	0.4832941440169094
1106	66-67	0.506922983156791
1106	68-69	0.5328672513951247
1106	70-71	0.46790563460714907
1106	72-73	0.4098938586303049
1106	74-75	0.46718568722080533
1106	76-77	0.482954570004388
1106	78-79	0.4632229213197192
1106	80-81	0.4891603394343278
1106	82-83	0.47366573535274625
1106	84-85	0.40464544004110437
1106	86-87	0.44605412957184853
1106	88-89	0.47822839833136044
1106	90-91	0.4898559721833422
1106	92-93	0.5452796029156737
1106	94-95	0.5409577525937337
1106	96-97	0.5165819584424725
1106	98-99	0.5335632398079042
1106	100	0.6045473094756382
1107	1	0.47735741937616893
1107	2	0.6651434652793782
1107	3	0.6058103968176383
1107	4	0.6858407264624091
1107	5	0.5249236412908616
1107	6	0.41679302229526627
1107	7	0.3609382230146636
1107	8	0.44190413901484504
1107	9	0.5413704854739478
1107	10-11	0.5997652684357533
1107	12-13	0.4523517194052573
1107	14-15	0.6660514634345844
1107	16-17	0.8099888724761328
1107	18-19	0.8875563450989361
1107	20-21	0.6185704910400887
1107	22-23	0.874123423790067
1107	24-25	0.6072837413438634
1107	26-27	0.521753128057064
1107	28-29	0.7040567700519347
1107	30-31	0.6867097101845872
1107	32-33	0.6665397810262732
1107	34-35	0.5658348376097209
1107	36-37	0.6070810272709792
1107	38-39	0.5963656987632859
1107	40-41	0.4936907571257976
1107	42-43	0.5672716438832168
1107	44-45	0.5398984081466267
1107	46-47	0.45565602873918465
1107	48-49	0.5221251875913921
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2214	3	-1.0970662545104517
2214	4	-0.8303171901108186
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2214	96-97	-1.0377148460393606
2214	98-99	-1.3372476013147434
2214	100	-1.3538551657559879
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2215	2	-2.3430247846550714
2215	3	-3.8827060711842165
2215	4	-2.1968888137512437
2215	5	-1.6793356179683983
2215	6	-1.5271004382373334
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2215	8	-1.3479015325125516
2215	9	-1.9480594036636916
2215	10-11	-1.5889693779005398
2215	12-13	-1.5864822593366057
2215	14-15	-3.90600421313507
2215	16-17	-3.7039268243969943
2215	18-19	-2.4514123962872105
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2215	22-23	-3.425375981223958
2215	24-25	-5.6976752147078535
2215	26-27	-3.848335750075016
2215	28-29	-2.0438910997637905
2215	30-31	-4.14046972010793
2215	32-33	-4.288254747119588
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2215	36-37	-2.2039819471210933
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2215	100	-3.3222074941749327
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2216	3	-0.5594239854927139
2216	4	-0.5963229827355647
2216	5	-0.6156329839498156
2216	6	-0.5006204478320839
2216	7	-0.6147387693658004
2216	8	-0.570984656771067
2216	9	-0.6988526562195929
2216	10-11	-0.7272418850479809
2216	12-13	-0.9559589402009081
2216	14-15	-1.3721239303492183
2216	16-17	-1.0295136115166912
2216	18-19	-2.928710971336969
2216	20-21	-1.172947784815939
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2216	24-25	-0.9866420933666689
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2216	28-29	-0.8307024008250465
2216	30-31	-0.9504900765399782
2216	32-33	-0.8481416036067415
2216	34-35	-1.150832622861131
2216	36-37	-1.1793268071220098
2216	38-39	-1.289345736881529
2216	40-41	-1.3202879249264612
2216	42-43	-1.2817478956783717
2216	44-45	-1.2407923203295468
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2216	50-51	-1.2052067796649624
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2216	54-55	-1.238611023989698
2216	56-57	-1.187691072333081
2216	58-59	-1.2395487497988995
2216	60-61	-1.215056960417705
2216	62-63	-1.3207766208875427
2216	64-65	-1.2199894511566285
2216	66-67	-1.1451573330621656
2216	68-69	-1.292575493832679
2216	70-71	-1.3938036054285519
2216	72-73	-1.3392354817432945
2216	74-75	-1.3597009978380754
2216	76-77	-1.2568933602796548
2216	78-79	-1.2571575590586583
2216	80-81	-1.2173982753496944
2216	82-83	-1.4166243179699052
2216	84-85	-1.2700082024070518
2216	86-87	-1.3958729424795067
2216	88-89	-1.3124675829875159
2216	90-91	-1.2746986542963796
2216	92-93	-1.2891584725080278
2216	94-95	-1.222237100441049
2216	96-97	-1.2110543128327826
2216	98-99	-1.1902461467211687
2216	100	-1.1456092549414763
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	14511.0
1	2418.0
2	398.0
3	352.0
4	354.0
5	189.0
6	179.0
7	254.0
8	516.0
9	886.0
10	1487.0
11	2463.0
12	4012.0
13	5482.0
14	7106.0
15	9325.0
16	11525.0
17	13651.0
18	15910.0
19	18124.0
20	20864.0
21	24163.0
22	27677.0
23	32294.0
24	36425.0
25	40754.0
26	45972.0
27	52532.0
28	62130.0
29	74235.0
30	88642.0
31	105152.0
32	130299.0
33	166710.0
34	231776.0
35	313089.0
36	458572.0
37	700022.0
38	572045.0
39	217620.0
40	223.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.03963314008914	21.69224823845837	12.685430546471407	23.582688074981085
2	15.156795322467472	19.871472019395096	41.43995751027617	23.531775147861264
3	18.22667388785329	27.350902724662106	29.326070926403435	25.09635246108116
4	11.911013645558926	17.051052639412184	40.9035617667902	30.13437194823869
5	12.732474350247763	38.947795790805486	35.331609187016085	12.988120671930664
6	29.01053700749216	39.13387384390503	18.524658635825826	13.330930512776984
7	26.279752471576405	32.217033169186706	24.247203165312808	17.256011193924085
8	25.914363530369787	35.31303221432081	20.170886967556616	18.601717287752788
9	26.006487360757568	15.057899136345402	22.089386060393107	36.846227442503924
10-11	25.454124698701325	26.14114010044817	27.72787001310592	20.67686518774459
12-13	25.620019331972937	24.09594386567859	27.743443239179467	22.54059356316901
14-15	23.114270985479852	25.197181888478394	25.266274307643844	26.422272818397914
16-17	21.77799353863074	27.77183003391073	26.757715837228485	23.692460590230045
18-19	21.17150588778785	28.469495930437454	28.405413405319607	21.95358477645509
20-21	23.170668839472512	27.501779247317526	27.060268154408597	22.26728375880137
22-23	23.66022394243877	27.163123318645948	26.81914044936502	22.357512289550264
24-25	22.936007748652234	27.298834609119215	27.683489207561696	22.081668434666856
26-27	22.811401763749434	27.544407632653805	26.602645552565086	23.04154505103168
28-29	21.857582142809587	28.143327027367164	27.20252918257297	22.796561647250275
30-31	22.750782450150346	27.62793931574504	27.127577854487967	22.493700379616648
32-33	23.348737206037036	27.852988233892585	26.0720926443143	22.72618191575608
34-35	23.218526269964983	27.648508949738403	27.180827031868787	21.952137748427827
36-37	22.812604690687046	27.33176193164102	26.86542601652822	22.990207361143717
38-39	23.034611629705786	27.2712105270017	27.50841263342171	22.1857652098708
40-41	22.582272324369054	26.425292408527945	28.155016657192668	22.83741860991033
42-43	22.50903119586438	26.72752770368741	27.397663632780635	23.365777467667574
44-45	22.707623292051572	27.752673240523972	27.155769480047464	22.38393398737699
46-47	22.70230499600325	27.36152414213796	26.72187210368035	23.214298758178444
48-49	23.38058319294882	26.908636350293786	27.002009652345176	22.708770804412215
50-51	23.013403345272653	26.82769928262394	26.865850118483834	23.293047253619566
52-53	22.317462895015737	27.56297433284632	26.683125790002837	23.436436982135106
54-55	22.416385482399352	27.447914257427925	26.657228059929523	23.478472200243203
56-57	23.606880903019718	26.74229920747499	26.444096386025233	23.20672350348006
58-59	22.42666707145439	27.191409984471044	27.468780269577163	22.9131426744974
60-61	22.465645930958935	27.889998245871535	26.618620252666098	23.025735570503436
62-63	22.877794096316414	27.05919648928525	26.239723294095814	23.82328612030252
64-65	22.022312811543994	27.28631710300905	26.72661371358509	23.96475637186186
66-67	22.856741492987513	26.783508777406528	26.663465709109712	23.696284020496247
68-69	22.13124181358939	26.949208984158197	26.950755853216453	23.968793349035963
70-71	22.168120403429853	26.53805196791487	27.213055734245422	24.08077189440985
72-73	22.826849870887365	26.6983670843058	27.553828675392182	22.92095436941465
74-75	22.996248674433776	27.008742113731255	27.096456826818216	22.898552385016753
76-77	22.595087077000972	26.980639500185	26.748227015348874	23.67604640746515
78-79	22.36549057893699	26.903731943266934	26.944854693171433	23.785922784624645
80-81	21.943813693892416	27.229535937363735	27.608640209367973	23.218010159375872
82-83	22.892795854870045	26.153903872942557	28.63596711298068	22.317333159206715
84-85	23.206613766918792	26.795906723522823	27.06186142801823	22.935618081540156
86-87	21.72062683326637	27.4957453926916	27.63020348134401	23.15342429269802
88-89	22.87193298478307	26.06832941024639	27.44317669504867	23.616560909921873
90-91	22.546443700609753	26.088909609106565	27.763545830303432	23.60110085998025
92-93	22.754636507410936	26.437630188098804	27.55790134372337	23.24983196076689
94-95	21.43141191451448	26.432350272800846	28.312235515111166	23.824002297573504
96-97	21.83706532489044	26.35458569269697	27.837238438104034	23.97111054430856
98-99	21.212888419208024	27.14185562152402	27.64726179993957	23.997994159328385
100	22.067201458061156	26.426263994783422	26.794494713145824	24.712039834009598
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	12232.0
1	7250.5
2	2080.5
3	1515.0
4	868.0
5	494.5
6	396.5
7	392.5
8	374.0
9	379.0
10	376.0
11	344.5
12	331.5
13	325.5
14	342.0
15	477.0
16	607.0
17	731.0
18	953.0
19	1149.5
20	1403.5
21	1817.0
22	2462.5
23	3327.0
24	4358.0
25	5712.5
26	7882.0
27	10511.0
28	14506.5
29	22472.5
30	32986.5
31	42980.5
32	56020.0
33	68127.5
34	77109.5
35	87415.5
36	96777.5
37	114400.0
38	121568.5
39	110357.0
40	100357.0
41	91004.0
42	88322.5
43	94440.0
44	103712.5
45	124398.0
46	144897.5
47	157620.5
48	164919.0
49	159532.5
50	150885.5
51	154403.5
52	156847.0
53	150688.0
54	141793.5
55	121319.0
56	104680.0
57	85395.0
58	66244.0
59	62920.5
60	51990.0
61	35531.0
62	26512.0
63	22362.5
64	16442.5
65	7837.0
66	4157.5
67	2930.5
68	2358.5
69	1902.5
70	1459.5
71	1124.0
72	862.0
73	691.5
74	554.0
75	408.5
76	304.5
77	215.0
78	143.5
79	111.0
80	86.5
81	57.0
82	39.5
83	29.0
84	22.5
85	20.5
86	18.5
87	13.5
88	11.5
89	13.0
90	9.5
91	5.5
92	4.5
93	5.5
94	6.5
95	3.0
96	1.0
97	1.5
98	2.5
99	2.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.000331022254837
2	0.45140952238787263
3	0.4818339430561957
4	0.46872979183201163
5	0.4351432824987223
6	0.49057953963407513
7	0.5050510805512175
8	0.5086119912099633
9	0.5218016042899573
10-11	0.535162141081571
12-13	0.5330825692568636
14-15	0.5309460228616162
16-17	0.5360737342102099
18-19	0.5340368933134074
20-21	0.5325128235514642
22-23	0.4965761131834029
24-25	0.5308320737205363
26-27	0.511304039667975
28-29	0.5168305730103483
30-31	0.5202490472427441
32-33	0.5229980702712959
34-35	0.5342932788808371
36-37	0.5526390905946948
38-39	0.5415860239099483
40-41	0.5470555826817817
42-43	0.54427807236796
44-45	0.5552314335542617
46-47	0.5520123703187556
48-49	0.5253197840207979
50-51	0.5393640156588909
52-53	0.5315015249243804
54-55	0.5413581256277886
56-57	0.5294931713128479
58-59	0.5354470139342707
60-61	0.5291655675322433
62-63	0.5241518053247294
64-65	0.534321766166107
66-67	0.53198580877397
68-69	0.5530948871590143
70-71	0.537725996755868
72-73	0.5414435874835984
74-75	0.5385663716713319
76-77	0.5266159555005814
78-79	0.5574819290905889
80-81	0.5323276561972095
82-83	0.5306041754383766
84-85	0.5399480050069252
86-87	0.846058128875339
88-89	1.1112035365255426
90-91	1.1021303361670585
92-93	1.1018454633143588
94-95	1.1067167890955232
96-97	1.100221688053971
98-99	1.0961052753324607
100	1.091917644397776
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3510338.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.394976376926564
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.62422406109313	22.229722797992338
2	12.47835254168622	7.33601756371656
3	4.6184800597968065	4.072803367651005
4	2.243135884812479	2.637477053771963
5	1.1986073230807759	1.7616516973585299
6	0.7641121134292231	1.3476634514145267
7	0.4991805642469988	1.0271380625702982
8	0.3425993694430635	0.805656029722303
9	0.2580515655554099	0.6826877706177171
>10	1.4407634083568683	8.122639388211512
>50	0.1982975672100957	4.135383212546728
>100	0.25226161929999774	16.333823448068298
>500	0.05119658084050283	10.568373746732606
>1k	0.02937985605051576	15.576145159606924
>5k	8.72668991599478E-4	1.7424320835486482
>10k+	4.8481610644415444E-4	1.620385166470224
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	12826	0.365377920872577	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	11917	0.33948297856217835	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	11224	0.3197412898700923	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	10824	0.3083463757621061	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	10060	0.2865820898158525	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	9572	0.27268029460410936	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	9195	0.2619405880573324	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	8226	0.23433640863073585	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6163	0.1755671391187971	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	6070	0.17291782158869032	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	5727	0.16314668274109217	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	5539	0.15779107311033866	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	5373	0.15306218375552438	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	5268	0.15007101880217802	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	4978	0.14180970607388804	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	4955	0.14115449851267883	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	4926	0.14032836723984984	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	4818	0.13725174043069358	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	4773	0.13596981259354513	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	4688	0.13354839334559807	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	4452	0.12682539402188622	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	4421	0.12594228817851727	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4384	0.12488825862352855	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	4374	0.12460338577082891	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	4310	0.12278019951355112	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	4122	0.11742458988279762	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	4036	0.1149746833495806	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	4017	0.11443342492945124	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	4006	0.11412006479148162	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	3846	0.10956209914828716	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	3819	0.10879294244599808	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	3778	0.1076249637499295	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	3762	0.10716916718561005	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	3653	0.10406405309118381	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	3643	0.10377918023848416	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	3611	0.10286758710984527	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	3563	0.10150019741688693	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.8487285269965456E-5
7	0.0	0.0	0.0	0.0	2.8487285269965456E-5
8	0.0	0.0	0.0	0.0	2.8487285269965456E-5
9	0.0	0.0	0.0	0.0	2.8487285269965456E-5
10-11	0.0	0.0	0.0	0.0	2.8487285269965456E-5
12-13	0.0	0.0	0.0	0.0	5.697457053993091E-5
14-15	0.0	0.0	0.0	0.0	7.121821317491364E-5
16-17	0.0	0.0	0.0	1.4243642634982728E-5	9.97054984448791E-5
18-19	0.0	0.0	0.0	2.8487285269965456E-5	1.1394914107986183E-4
20-21	0.0	0.0	0.0	7.121821317491364E-5	1.1394914107986183E-4
22-23	0.0	0.0	0.0	1.5668006898481002E-4	1.1394914107986183E-4
24-25	0.0	0.0	0.0	3.988219937795164E-4	1.1394914107986183E-4
26-27	0.0	0.0	0.0	7.406694170191019E-4	1.4243642634982729E-4
28-29	0.0	0.0	0.0	0.0016949934735629446	1.4243642634982729E-4
30-31	0.0	0.0	0.0	0.005626238840818178	1.4243642634982729E-4
32-33	0.0	0.0	0.0	0.016323214459690205	1.4243642634982729E-4
34-35	0.0	0.0	0.0	0.03452658974719813	1.4243642634982729E-4
36-37	0.0	0.0	0.0	0.0650649595566011	1.4243642634982729E-4
38-39	0.0	0.0	0.0	0.11562989091078979	1.994109968897582E-4
40-41	0.0	0.0	0.0	0.17199198481741645	2.2789828215972365E-4
42-43	0.0	0.0	0.0	0.22919445363950708	2.2789828215972365E-4
44-45	0.0	0.0	0.0	0.3004269104570557	2.2789828215972365E-4
46-47	0.0	0.0	0.0	0.39244084187904416	2.2789828215972365E-4
48-49	0.0	0.0	0.0	0.5054071716170921	2.2789828215972365E-4
50-51	0.0	0.0	0.0	0.6281019092748333	2.2789828215972365E-4
52-53	0.0	0.0	0.0	0.7475348527691635	3.7033470850955094E-4
54-55	0.0	0.0	0.0	0.8797016127791684	3.988219937795164E-4
56-57	0.0	0.0	0.0	1.0412530075451425	3.988219937795164E-4
58-59	0.0	0.0	0.0	1.2430142054696727	3.988219937795164E-4
60-61	0.0	0.0	0.0	1.457110397916098	3.988219937795164E-4
62-63	0.0	0.0	0.0	1.6797527759435131	3.988219937795164E-4
64-65	0.0	0.0	0.0	1.910770415840298	4.2730927904948183E-4
66-67	0.0	0.0	0.0	2.1738362516657936	4.557965643194473E-4
68-69	0.0	0.0	0.0	2.4778668037095004	4.557965643194473E-4
70-71	0.0	0.0	0.0	2.817064909418979	4.7004020695443007E-4
72-73	0.0	0.0	0.0	3.1734835790741522	5.127711348593782E-4
74-75	0.0	0.0	0.0	3.5193049786088975	5.555020627643264E-4
76-77	0.0	0.0	0.0	3.9166456335543756	5.697457053993091E-4
78-79	0.0	0.0	0.0	4.352558072755388	5.697457053993091E-4
80-81	0.0	0.0	0.0	4.83266853505275	5.697457053993091E-4
82-83	0.0	0.0	0.0	5.326338945138616	5.982329906692746E-4
84-85	0.0	0.0	0.0	5.804027988188032	5.982329906692746E-4
86-87	0.0	0.0	0.0	6.316670930263696	5.982329906692746E-4
88	0.0	0.0	0.0	6.728639806195301	5.982329906692746E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	3585	0.0	42.1326	94
GTACTAG	3900	0.0	35.68381	1
GTATCAA	8035	0.0	33.81819	1
CAACGCA	7390	0.0	32.558334	5
GGTATCA	5090	0.0	32.438744	1
GCATCGT	4060	0.0	31.804518	8
CTGTGCG	5210	0.0	30.823132	9
ATCAACG	7840	0.0	30.632248	3
TAGTACT	5370	0.0	30.192142	4
TAGGCAT	5000	0.0	29.417856	5
TATTCTC	2195	0.0	28.902536	5
ACTGTGC	5990	0.0	27.828106	8
TATCAAC	9740	0.0	27.60092	2
GTACTGT	5970	0.0	27.447853	6
CTAGTAC	6420	0.0	27.158945	3
AACGCAG	9360	0.0	27.138052	6
CTAGGCA	5605	0.0	27.081707	4
TACTGTG	6525	0.0	26.841343	7
CGTTAAC	795	0.0	26.702946	1
ACGCAGA	9025	0.0	26.585793	7
>>END_MODULE