##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146478_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3510338 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84378114016371 31.0 31.0 34.0 30.0 34.0 2 31.80503643808659 31.0 31.0 34.0 30.0 34.0 3 31.96974792740756 33.0 31.0 34.0 30.0 34.0 4 35.715210330173335 37.0 35.0 37.0 35.0 37.0 5 35.63366576096091 37.0 35.0 37.0 33.0 37.0 6 35.67842327433996 37.0 35.0 37.0 33.0 37.0 7 35.59554350606694 37.0 35.0 37.0 33.0 37.0 8 35.58519464507406 37.0 35.0 37.0 33.0 37.0 9 37.16155652247732 39.0 37.0 39.0 33.0 39.0 10-11 37.10625814380268 39.0 37.0 39.0 33.0 39.0 12-13 37.04031819727901 39.0 37.0 39.0 33.0 39.0 14-15 38.22519056569482 40.0 37.0 41.0 33.0 41.0 16-17 38.23537078195889 40.0 37.0 41.0 33.0 41.0 18-19 38.300205421814084 40.0 38.0 41.0 33.0 41.0 20-21 38.317502331684295 40.0 38.0 41.0 33.0 41.0 22-23 38.285981008096655 40.0 37.5 41.0 33.0 41.0 24-25 38.21312135754449 40.0 37.0 41.0 33.0 41.0 26-27 38.062144585507156 40.0 37.0 41.0 33.0 41.0 28-29 38.000287436708376 40.0 37.0 41.0 33.0 41.0 30-31 37.86427346882266 40.0 37.0 41.0 33.0 41.0 32-33 37.723796540390126 40.0 36.0 41.0 33.0 41.0 34-35 37.618903079988314 40.0 36.0 41.0 32.0 41.0 36-37 37.53444753753057 40.0 36.0 41.0 32.0 41.0 38-39 37.41375104049809 40.0 36.0 41.0 31.5 41.0 40-41 37.27959729234051 39.5 36.0 41.0 31.0 41.0 42-43 37.14610701305686 39.0 35.0 41.0 31.0 41.0 44-45 36.92367643799543 39.0 35.0 41.0 31.0 41.0 46-47 36.661546267054625 39.0 35.0 41.0 30.0 41.0 48-49 36.372305031595246 39.0 35.0 41.0 29.5 41.0 50-51 36.159069867346105 39.0 35.0 41.0 29.0 41.0 52-53 35.96502388089124 38.0 35.0 40.5 29.0 41.0 54-55 35.76793502506027 38.0 34.5 40.0 28.5 41.0 56-57 35.50360136260383 37.5 34.0 40.0 28.0 41.0 58-59 35.212538080378586 37.0 34.0 40.0 28.0 41.0 60-61 34.95457502952707 36.5 34.0 40.0 27.0 41.0 62-63 34.68393157581976 36.0 34.0 39.5 27.0 41.0 64-65 34.41595453201373 35.5 33.0 39.0 26.0 41.0 66-67 34.20189337892818 35.0 33.0 39.0 26.0 41.0 68-69 34.021429275471476 35.0 33.0 38.5 26.0 41.0 70-71 33.831716917288304 35.0 33.0 38.0 26.0 40.5 72-73 33.64071593675595 35.0 33.0 37.0 26.0 40.0 74-75 33.466874699815236 35.0 33.0 37.0 26.0 39.5 76-77 33.31107759423736 35.0 33.0 36.0 26.0 39.0 78-79 33.198802793349245 35.0 33.0 36.0 26.0 39.0 80-81 33.05799911575467 35.0 33.0 36.0 26.0 38.5 82-83 32.86309680150458 35.0 33.0 35.5 26.0 37.0 84-85 32.722155815195 35.0 33.0 35.0 26.0 37.0 86-87 32.58038428208337 35.0 33.0 35.0 26.0 36.5 88-89 32.46673041171534 35.0 33.0 35.0 26.0 36.0 90-91 32.3734264335799 35.0 33.0 35.0 26.0 36.0 92-93 32.286997434435094 35.0 33.0 35.0 26.0 36.0 94-95 32.24323669116763 35.0 33.0 35.0 26.0 35.5 96-97 32.191782814076596 35.0 33.0 35.0 25.0 35.0 98-99 32.15661269655514 35.0 33.0 35.0 25.0 35.0 100 31.662443616540628 34.0 32.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.46722894807138005 1101 2 0.533913200620237 1101 3 0.3911700937180953 1101 4 0.26510897902824837 1101 5 0.33057466268851954 1101 6 0.3674785813470791 1101 7 0.31890784066202826 1101 8 0.3471718141150433 1101 9 0.4475962553719768 1101 10-11 0.42197650064937875 1101 12-13 0.4436049179866757 1101 14-15 0.5790499868813512 1101 16-17 0.5838597408585997 1101 18-19 0.5965826264395702 1101 20-21 0.5608946149642264 1101 22-23 0.620010758654594 1101 24-25 0.6571306717313519 1101 26-27 0.6791115337576556 1101 28-29 0.6904007380374608 1101 30-31 0.6958643461380376 1101 32-33 0.7034676862558555 1101 34-35 0.7033693325617634 1101 36-37 0.7006230966743274 1101 38-39 0.6820447502231204 1101 40-41 0.687900374222238 1101 42-43 0.7517593871227319 1101 44-45 0.7210939421378484 1101 46-47 0.7290197378832133 1101 48-49 0.6821487243776616 1101 50-51 0.7148263351071407 1101 52-53 0.7232397455681365 1101 54-55 0.6701004523025418 1101 56-57 0.7226367344731273 1101 58-59 0.6810093552203824 1101 60-61 0.7149268393504258 1101 62-63 0.7520707059912866 1101 64-65 0.7006948516206961 1101 66-67 0.6152998601209916 1101 68-69 0.6234226074970906 1101 70-71 0.5768793696093155 1101 72-73 0.6581060987500109 1101 74-75 0.5535721478808568 1101 76-77 0.560823468999665 1101 78-79 0.5826411651026575 1101 80-81 0.5569788691812931 1101 82-83 0.5176059466540153 1101 84-85 0.5654844629145614 1101 86-87 0.547371657813045 1101 88-89 0.5299647088826731 1101 90-91 0.5387898327345795 1101 92-93 0.5284765716237345 1101 94-95 0.5579878098054607 1101 96-97 0.6162784472890834 1101 98-99 0.6009790950728942 1101 100 0.5679333229997106 1102 1 0.4149815847807332 1102 2 0.5107653382451183 1102 3 0.4193486691471193 1102 4 0.31117884237998794 1102 5 0.31518833163955406 1102 6 0.349387387017714 1102 7 0.3596076691683905 1102 8 0.3329811430241847 1102 9 0.46319767738065565 1102 10-11 0.4525734103738941 1102 12-13 0.507364997528299 1102 14-15 0.6807977395321814 1102 16-17 0.672557411887162 1102 18-19 0.6461686849293429 1102 20-21 0.5914086629358266 1102 22-23 0.566255745809876 1102 24-25 0.621139961570293 1102 26-27 0.652585832241904 1102 28-29 0.6621514484979798 1102 30-31 0.6962903081642224 1102 32-33 0.7282404900291866 1102 34-35 0.7591777030784215 1102 36-37 0.7023113717839635 1102 38-39 0.7501234049366943 1102 40-41 0.7134723445543116 1102 42-43 0.7719891315292671 1102 44-45 0.7730747458965013 1102 46-47 0.7241844994652453 1102 48-49 0.747739178195225 1102 50-51 0.7183031916588831 1102 52-53 0.7408272011780355 1102 54-55 0.7021497998399511 1102 56-57 0.7459778114576778 1102 58-59 0.7471536744389482 1102 60-61 0.6856893885360122 1102 62-63 0.6741010452932983 1102 64-65 0.6939055201322972 1102 66-67 0.7336778059066305 1102 68-69 0.6917956306625044 1102 70-71 0.6888174581608268 1102 72-73 0.7247028293674234 1102 74-75 0.6266002959459271 1102 76-77 0.5818734088052722 1102 78-79 0.5432099883478259 1102 80-81 0.5921711896677451 1102 82-83 0.6712573090333009 1102 84-85 0.6052456388515495 1102 86-87 0.576694813069949 1102 88-89 0.5812488418157358 1102 90-91 0.6579321971714762 1102 92-93 0.6276642969112984 1102 94-95 0.6298136414281217 1102 96-97 0.6258115745456649 1102 98-99 0.5738626085044416 1102 100 0.6451178540223204 1103 1 0.2454486667459861 1103 2 -0.19284189645686212 1103 3 0.19571784017117722 1103 4 0.2119075887172741 1103 5 0.22446546827375613 1103 6 0.26695102940515625 1103 7 0.2708678013707555 1103 8 0.26430221162483747 1103 9 0.3652113627704807 1103 10-11 0.36348264630542104 1103 12-13 0.40633160909886357 1103 14-15 0.5038915746140518 1103 16-17 0.5196399799850298 1103 18-19 0.495556761949949 1103 20-21 0.457083764110493 1103 22-23 0.4357725155068337 1103 24-25 0.3902706365530406 1103 26-27 0.39109199347478096 1103 28-29 0.48671020477998894 1103 30-31 0.5716060794426738 1103 32-33 0.4898070362156659 1103 34-35 0.5410339175097789 1103 36-37 0.5391601847124718 1103 38-39 0.5452445067698477 1103 40-41 0.5403152273576026 1103 42-43 0.6012430569228542 1103 44-45 0.5895418682688742 1103 46-47 0.6241002974182024 1103 48-49 0.5610873354511554 1103 50-51 0.5060964592746657 1103 52-53 0.5854498318982309 1103 54-55 0.5197182519075199 1103 56-57 0.5607859931806018 1103 58-59 0.5043917624191749 1103 60-61 0.4529303960573614 1103 62-63 0.4904901463840332 1103 64-65 0.3858991183972691 1103 66-67 0.42699724001403183 1103 68-69 0.4258710463742901 1103 70-71 0.37635785847638203 1103 72-73 0.4096176739082935 1103 74-75 0.44470425112435663 1103 76-77 0.48209993168112675 1103 78-79 0.3987324206269989 1103 80-81 0.4304439725983542 1103 82-83 0.33363347019835743 1103 84-85 0.4352471140377574 1103 86-87 0.44582387031714177 1103 88-89 0.3991859272097855 1103 90-91 0.38098813153871447 1103 92-93 0.3920025210170692 1103 94-95 0.36369407648000873 1103 96-97 0.4033172954715596 1103 98-99 0.4499144494566707 1103 100 0.46970207331674274 1104 1 0.36449900386447354 1104 2 0.42002352602807136 1104 3 0.3366202608224711 1104 4 0.24635813090666403 1104 5 0.24651168089313558 1104 6 0.31677458821209825 1104 7 0.2748313913359368 1104 8 0.26998365368351784 1104 9 0.3315732559989968 1104 10-11 0.3659144678068813 1104 12-13 0.4068962617495018 1104 14-15 0.4737111802468661 1104 16-17 0.45029431923553886 1104 18-19 0.4877344324888071 1104 20-21 0.4573924674226646 1104 22-23 0.4360339668996218 1104 24-25 0.5004246189832173 1104 26-27 0.5375958860744063 1104 28-29 0.5350380526112986 1104 30-31 0.540821133879966 1104 32-33 0.5462053769206605 1104 34-35 0.5277460583670717 1104 36-37 0.5340923428254598 1104 38-39 0.5772995444767375 1104 40-41 0.5806057503597017 1104 42-43 0.5431282482889799 1104 44-45 0.5630455664279665 1104 46-47 0.566794965550379 1104 48-49 0.507527345393612 1104 50-51 0.42748329739873014 1104 52-53 0.4130689300063324 1104 54-55 0.42425709030342773 1104 56-57 0.4898264830059986 1104 58-59 0.46531027259989344 1104 60-61 0.4227358140029196 1104 62-63 0.40013509881842424 1104 64-65 0.38691263832890854 1104 66-67 0.3989292538624625 1104 68-69 0.3554117559877312 1104 70-71 0.3828512168912397 1104 72-73 0.3586939118610033 1104 74-75 0.30811775953974774 1104 76-77 0.28459865677901064 1104 78-79 0.3145345524965748 1104 80-81 0.34179494251883114 1104 82-83 0.3989606934599905 1104 84-85 0.3120152329341792 1104 86-87 0.3323350027897618 1104 88-89 0.3048801797119367 1104 90-91 0.3161231154445119 1104 92-93 0.35370763464366206 1104 94-95 0.3486526710989253 1104 96-97 0.26632375567923106 1104 98-99 0.3135042658392706 1104 100 0.4348030963977969 1105 1 0.18252528878626606 1105 2 -0.3223140213737281 1105 3 0.023807327305870984 1105 4 0.09940874022132107 1105 5 0.12431647397282575 1105 6 0.1970247982782567 1105 7 0.21987268830409334 1105 8 0.21675908343791406 1105 9 0.36685212255385125 1105 10-11 0.2830434857992188 1105 12-13 0.26315598091869674 1105 14-15 0.38174518673452695 1105 16-17 0.3699755464175212 1105 18-19 0.37239277908859947 1105 20-21 0.31926394676501957 1105 22-23 0.3268828757969615 1105 24-25 0.35938169180344914 1105 26-27 0.36665544024426566 1105 28-29 0.3830067470785252 1105 30-31 0.4066350959208265 1105 32-33 0.4147488879309904 1105 34-35 0.4005646263963172 1105 36-37 0.40721590341259173 1105 38-39 0.3796852773172006 1105 40-41 0.42544992236315693 1105 42-43 0.41950642366012403 1105 44-45 0.43465724149927354 1105 46-47 0.35943447848632815 1105 48-49 0.4967927955950202 1105 50-51 0.4911853361656071 1105 52-53 0.445267882868535 1105 54-55 0.4528792262643222 1105 56-57 0.4231333663916388 1105 58-59 0.40586330983985874 1105 60-61 0.4780142298233159 1105 62-63 0.47475705939781676 1105 64-65 0.502468257832561 1105 66-67 0.47634127069536447 1105 68-69 0.39681128448895464 1105 70-71 0.40884664635089507 1105 72-73 0.3852677949390255 1105 74-75 0.3608813061016818 1105 76-77 0.31725363807186824 1105 78-79 0.35644645123572616 1105 80-81 0.364782399886586 1105 82-83 0.3606145092159423 1105 84-85 0.4114728447106799 1105 86-87 0.3943930722575715 1105 88-89 0.4122624035945961 1105 90-91 0.3421174661950488 1105 92-93 0.3562941135923765 1105 94-95 0.3209892586155547 1105 96-97 0.3109830269816811 1105 98-99 0.3754815482915177 1105 100 0.338670630386428 1106 1 0.23311138018294741 1106 2 0.23341428571537293 1106 3 0.22523520160574861 1106 4 0.1553743318200489 1106 5 0.13463737711417423 1106 6 0.20120888381556057 1106 7 0.20021528392015853 1106 8 0.21740765662387673 1106 9 0.28541001923603915 1106 10-11 0.23336777910272843 1106 12-13 0.18031912650066317 1106 14-15 0.32869844289402295 1106 16-17 0.3144248839602426 1106 18-19 0.33299150508015174 1106 20-21 0.35539841501083913 1106 22-23 0.28645478930997825 1106 24-25 0.26618378846977464 1106 26-27 0.3606653149303938 1106 28-29 0.331118577066853 1106 30-31 0.33214198623082325 1106 32-33 0.2888009577887303 1106 34-35 0.3468257579991558 1106 36-37 0.3515424884979552 1106 38-39 0.28324059916719335 1106 40-41 0.32631098127749425 1106 42-43 0.3106236015420478 1106 44-45 0.3139605229105129 1106 46-47 0.2677279747299721 1106 48-49 0.30494561889818783 1106 50-51 0.38499523356018983 1106 52-53 0.35388108576518107 1106 54-55 0.284539522620328 1106 56-57 0.2840366802013392 1106 58-59 0.3807362492191686 1106 60-61 0.3189763842861453 1106 62-63 0.23121371786555756 1106 64-65 0.35016846026255166 1106 66-67 0.34139805322746497 1106 68-69 0.3122593515932266 1106 70-71 0.31051657635062213 1106 72-73 0.3171533169736662 1106 74-75 0.33477108503116426 1106 76-77 0.3286701031205226 1106 78-79 0.25720532548178454 1106 80-81 0.2899453752893848 1106 82-83 0.3654323712100336 1106 84-85 0.3080974926928022 1106 86-87 0.32823347373006584 1106 88-89 0.32787338570686586 1106 90-91 0.2984516305610114 1106 92-93 0.3416143825881832 1106 94-95 0.27927134023955347 1106 96-97 0.25160554785848177 1106 98-99 0.26836554287424264 1106 100 0.37816417424802395 1107 1 0.06390604891311469 1107 2 -0.5712819468687549 1107 3 -0.04149148427396909 1107 4 0.052678783986294775 1107 5 0.057029851544726284 1107 6 0.16404154719291597 1107 7 0.16235231949820417 1107 8 0.18934261502909777 1107 9 0.2287935173848865 1107 10-11 0.23560585870590245 1107 12-13 0.229522580920289 1107 14-15 0.2712275522837686 1107 16-17 0.3311997683686485 1107 18-19 0.33503031355648716 1107 20-21 0.2623040899051148 1107 22-23 0.23487680952926837 1107 24-25 0.28034860182651755 1107 26-27 0.31749034360806405 1107 28-29 0.22045251377863906 1107 30-31 0.30012296774569336 1107 32-33 0.3021365323110743 1107 34-35 0.3275947016195886 1107 36-37 0.33497417627577875 1107 38-39 0.3918993161063824 1107 40-41 0.3491442781824361 1107 42-43 0.33078668842073 1107 44-45 0.30516071917881504 1107 46-47 0.33836333467368007 1107 48-49 0.3429063647770505 1107 50-51 0.2860482440968184 1107 52-53 0.2805223317801264 1107 54-55 0.2527540061883826 1107 56-57 0.28093346626181415 1107 58-59 0.281772877694749 1107 60-61 0.3317623027542993 1107 62-63 0.3336181792182771 1107 64-65 0.37784471632411964 1107 66-67 0.32077892207199454 1107 68-69 0.3166747290680547 1107 70-71 0.35112701347708253 1107 72-73 0.33813568819493156 1107 74-75 0.3254053255889673 1107 76-77 0.26699317602072625 1107 78-79 0.29887744871523125 1107 80-81 0.2538780100129756 1107 82-83 0.2553467668654221 1107 84-85 0.2845772534474662 1107 86-87 0.28315773834115987 1107 88-89 0.2687731062987808 1107 90-91 0.24955816626831506 1107 92-93 0.19208224205567603 1107 94-95 0.22062939595984687 1107 96-97 0.22820708632848863 1107 98-99 0.2339932264873994 1107 100 0.2757699640441622 1108 1 0.12330669248113679 1108 2 0.2531386471293935 1108 3 0.15548656798945615 1108 4 0.1291003983793999 1108 5 0.07001202466336309 1108 6 0.1453467180335224 1108 7 0.1557138545996537 1108 8 0.13599487604490434 1108 9 0.1371524036292726 1108 10-11 0.1641397811955443 1108 12-13 0.08706853678916104 1108 14-15 0.22657036934787556 1108 16-17 0.16722614357624366 1108 18-19 0.16552099512026075 1108 20-21 0.11253828852750303 1108 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-0.8755549912981948 2215 20-21 -0.806808977600582 2215 22-23 -0.7951831092568256 2215 24-25 -0.7153563389101976 2215 26-27 -0.8447005998312918 2215 28-29 -0.8682749725568542 2215 30-31 -0.9432259125377414 2215 32-33 -0.972525687087483 2215 34-35 -0.9564153358275505 2215 36-37 -0.8755957497039333 2215 38-39 -0.9452124807512874 2215 40-41 -1.0077060273781626 2215 42-43 -0.9128387763070194 2215 44-45 -1.001950707913558 2215 46-47 -1.0447656529089286 2215 48-49 -0.9821798577678678 2215 50-51 -0.9805983543985732 2215 52-53 -1.049751210504212 2215 54-55 -1.0482951188124616 2215 56-57 -0.9273772273506609 2215 58-59 -0.8569261703276752 2215 60-61 -0.87952600997302 2215 62-63 -0.844864152723396 2215 64-65 -0.9438249588874896 2215 66-67 -0.8895542700782215 2215 68-69 -0.9014167273366169 2215 70-71 -0.8215528773413112 2215 72-73 -0.874312561470596 2215 74-75 -0.8055281132955443 2215 76-77 -0.815703371043675 2215 78-79 -0.7420238205882015 2215 80-81 -0.7656678341612064 2215 82-83 -0.7755499812168409 2215 84-85 -0.8270126196894196 2215 86-87 -0.7263981735336813 2215 88-89 -0.7501425401518098 2215 90-91 -0.7278550229237801 2215 92-93 -0.7736481956792076 2215 94-95 -0.7983901041174661 2215 96-97 -0.8647345142846454 2215 98-99 -0.7980503150574165 2215 100 -0.8033544989557768 2216 1 -0.3561890760478157 2216 2 -0.11357839177724571 2216 3 -0.30967171046653874 2216 4 -0.15991126605577932 2216 5 -0.2969406749241088 2216 6 -0.3678004488953377 2216 7 -0.38292287695085747 2216 8 -0.44579247588096393 2216 9 -0.523049948538528 2216 10-11 -0.5666590378081438 2216 12-13 -0.5116345701629825 2216 14-15 -0.6534536744357027 2216 16-17 -0.7577749523412223 2216 18-19 -0.6769201040041111 2216 20-21 -0.6311644436915671 2216 22-23 -0.6328062527048459 2216 24-25 -0.6951329367928665 2216 26-27 -0.7214534409798077 2216 28-29 -0.7632122987330163 2216 30-31 -0.7011952449893926 2216 32-33 -0.7183817755407205 2216 34-35 -0.6690539248804583 2216 36-37 -0.7818913814988662 2216 38-39 -0.76762763527217 2216 40-41 -0.8240077369294738 2216 42-43 -0.8341199835601287 2216 44-45 -0.7553981734081034 2216 46-47 -0.7368387350931656 2216 48-49 -0.7158924430995697 2216 50-51 -0.8158963103275454 2216 52-53 -0.7679879318220628 2216 54-55 -0.8359681322775216 2216 56-57 -0.7679233016062597 2216 58-59 -0.7971893614901333 2216 60-61 -0.7873468506806915 2216 62-63 -0.7745527284862774 2216 64-65 -0.7064996741243448 2216 66-67 -0.674242019266238 2216 68-69 -0.7860419971890025 2216 70-71 -0.8323228237273952 2216 72-73 -0.8088813487069899 2216 74-75 -0.7848481663259932 2216 76-77 -0.8055244905177759 2216 78-79 -0.7518288299560538 2216 80-81 -0.5794922015883301 2216 82-83 -0.537428510367377 2216 84-85 -0.5590509046924339 2216 86-87 -0.5744777645898154 2216 88-89 -0.5952133014434722 2216 90-91 -0.6746748814660251 2216 92-93 -0.6801909571523908 2216 94-95 -0.6408271223539757 2216 96-97 -0.5383329836624782 2216 98-99 -0.6248219891470512 2216 100 -0.6789405891594065 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 0.0 11 4.0 12 10.0 13 64.0 14 239.0 15 712.0 16 1797.0 17 3554.0 18 6304.0 19 9611.0 20 14148.0 21 20168.0 22 27350.0 23 31836.0 24 33333.0 25 33485.0 26 35726.0 27 42748.0 28 53776.0 29 67650.0 30 83106.0 31 102630.0 32 129057.0 33 162608.0 34 222552.0 35 306374.0 36 449803.0 37 758472.0 38 641242.0 39 271289.0 40 689.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.987629217270204 22.189172687522632 13.886502769465533 23.936695325741628 2 14.836546378095647 20.46131668836552 40.5384924376882 24.16364449585063 3 17.877405790991034 27.048098727167062 30.268895928529915 24.805599553311986 4 12.193894561070062 17.58158964816646 39.4771936523496 30.747322138413885 5 12.251819388156841 39.083443154696276 35.31347945414652 13.35125800300036 6 28.70663736654419 39.6201733280385 17.893348161914894 13.77984114350242 7 25.26023928824864 32.95920864352524 24.280131167267324 17.500420900958797 8 26.00883447690792 35.137100757818764 19.777611158811485 19.076453606461826 9 25.88990006090582 14.458978024338398 22.048788464244755 37.60233345051103 10-11 25.453147246789342 26.18871743974512 27.415849983676786 20.942285329788753 12-13 25.867395105542546 24.130881413698624 27.99449796572296 22.007225515035874 14-15 23.146871117368747 24.996374667630384 26.04883215864967 25.807922056351195 16-17 21.24227796065517 27.473096607216874 27.485517392660626 23.79910803946733 18-19 20.71129617717724 27.268200384122554 30.39131844283941 21.629184995860797 20-21 22.397933530560472 26.37899231839696 29.532608711134404 21.69046543990816 22-23 22.77852700021024 26.075903354294468 29.257691494701604 21.88787815079369 24-25 22.163182315090786 26.200144572993338 30.152941134577517 21.483731977338362 26-27 22.093613042749357 26.426335275551455 29.058805951058496 22.421245730640692 28-29 21.18814484531119 27.053719613324983 29.642914727869513 22.115220813494314 30-31 22.170941943482365 26.48025631719795 29.448574467757805 21.900227271561885 32-33 22.782450009087444 26.780426842087575 28.520886022941383 21.916237125883605 34-35 22.54559413386814 26.615151894983935 29.455890061304952 21.383363909842977 36-37 22.25490821681559 26.239666949450452 29.144714839425717 22.36070999430824 38-39 22.445844246337533 26.089923534428877 29.856669072892693 21.607563146340894 40-41 21.943277095473697 25.489384467776233 30.23079324781046 22.336545188939606 42-43 22.152046490421302 25.69168659959101 29.308413818761824 22.847853091225872 44-45 22.356664799799905 26.740444937211173 28.878287503938367 22.024602759050552 46-47 22.446997981390965 26.457324052555624 28.1413784085749 22.95429955747851 48-49 23.363728934799845 26.222868760026678 28.070092725125033 22.343309580048437 50-51 22.747310942706942 26.324060532062727 27.678559728436408 23.250068796793926 52-53 22.01441855456654 27.037538835291645 27.49464581473351 23.453396795408306 54-55 22.185438670208598 26.91974389835699 27.337782114191704 23.557035317242704 56-57 23.328959106318788 26.254449540350073 27.120492363334602 23.296098989996537 58-59 22.112335512376678 26.69788403235306 28.14563862540087 23.044141829869393 60-61 22.24862837184651 27.36350984115336 27.29318892738315 23.09467285961698 62-63 22.7400466849631 26.502191526855817 26.85435134736313 23.90341044081795 64-65 21.808412751136785 26.690891304484072 27.38616623242548 24.114529711953665 66-67 22.522916596635422 26.24852649516941 27.362365105582427 23.86619180261274 68-69 21.983941780300455 26.18363813013143 27.72734271632012 24.10507737324799 70-71 22.005750485996685 25.812249737345283 27.924597585627716 24.257402191030312 72-73 22.66560940855268 25.927716932101696 28.188624571195138 23.218049088150487 74-75 22.739740466180912 26.411529392158723 27.648491987582375 23.200238154077997 76-77 22.4751825661765 26.396972395572487 27.100159134690166 24.027685903560844 78-79 22.07534414963426 26.40668839498633 27.429729320896477 24.088238134482935 80-81 21.691483242792977 26.836543909913363 28.020301285873455 23.451671561420206 82-83 22.5899726132685 25.749196680090584 29.063451269437035 22.59737943720388 84-85 22.98969114751455 26.312052928965624 27.43844722936146 23.259808694158366 86-87 21.45318541350284 26.992415644828306 27.979992903244096 23.57440603842476 88-89 22.35180042301223 25.81310483227656 27.753625005128114 24.081469739583095 90-91 21.996697540334196 25.669359174111587 28.132372452039267 24.20157083351495 92-93 22.239007504089365 26.14751855367185 27.69394870883196 23.919525233406823 94-95 20.878034745066813 26.018988500213304 28.55455365771843 24.54842309700145 96-97 21.277663158578463 25.952915724405685 28.041686822362657 24.7277342946532 98-99 20.621072148754433 26.71408184302711 27.923861094956127 24.740984913262334 100 21.485722040748716 26.02324400503051 27.02880396555966 25.46222998866111 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 255.0 1 180.5 2 149.0 3 198.0 4 145.5 5 112.5 6 230.5 7 567.0 8 978.5 9 1150.5 10 918.5 11 632.5 12 604.5 13 691.5 14 866.0 15 1196.5 16 1575.5 17 2077.0 18 2806.5 19 3733.0 20 4750.5 21 5686.0 22 6757.5 23 7838.5 24 8920.5 25 10209.0 26 12001.0 27 14086.5 28 17668.5 29 25142.0 30 35156.5 31 43852.5 32 56388.5 33 68227.5 34 75570.5 35 85234.0 36 93751.5 37 111338.5 38 119039.0 39 107925.5 40 98141.0 41 88568.5 42 86077.0 43 91871.5 44 100557.5 45 122659.5 46 144046.5 47 157291.0 48 166115.5 49 159698.5 50 149626.5 51 152248.0 52 154345.0 53 148632.5 54 142268.5 55 122388.5 56 105260.5 57 86414.5 58 66708.5 59 64476.5 60 53299.5 61 35704.5 62 26073.0 63 21758.5 64 15714.5 65 6938.0 66 3523.0 67 2575.0 68 2114.5 69 1691.5 70 1256.5 71 979.5 72 775.5 73 614.5 74 475.5 75 341.5 76 234.0 77 153.0 78 105.0 79 72.0 80 40.0 81 11.0 82 4.0 83 0.5 84 0.5 85 0.5 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.012961714797834282 2 0.0047858639253541965 3 0.0016522625456579967 4 0.028715183552125184 5 0.002620830244836822 6 0.0 7 3.1336013796962E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.008973494860039118 16-17 0.0026493175301067875 18-19 0.0 20-21 8.546185580989637E-5 22-23 9.115931286388946E-4 24-25 1.4243642634982728E-5 26-27 1.2819278371484455E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 5.127711348593782E-4 36-37 0.0 38-39 0.0 40-41 2.8487285269965457E-4 42-43 6.694512038441882E-4 44-45 0.0 46-47 0.0 48-49 4.7004020695443007E-4 50-51 0.0 52-53 0.0 54-55 3.7033470850955094E-4 56-57 9.97054984448791E-5 58-59 1.5668006898481E-4 60-61 7.121821317491364E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0013816333355933246 70-71 7.406694170191019E-4 72-73 0.0 74-75 1.5668006898481E-4 76-77 0.0029626776680764075 78-79 5.412584201293437E-4 80-81 0.0033330123765859584 82-83 0.0017519680441028754 84-85 0.0056832134113581085 86-87 0.008076145374035205 88-89 0.007805516163970534 90-91 0.006466613756282159 92-93 0.009073200358483998 94-95 0.0015810443324830827 96-97 0.004671914784274335 98-99 0.0019798663262625992 100 0.08372413140842848 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3510338.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.055747591845584 #Duplication Level Percentage of deduplicated Percentage of total 1 69.21113415025503 15.957144395136444 2 15.42585534901439 7.113092546303937 3 5.948020638292496 4.114081875226802 4 2.8783189229773543 2.654471783079949 5 1.55484486993099 1.7924055432802444 6 0.9958749359529182 1.3776384693825523 7 0.6443466397243384 1.0399125440996746 8 0.4621671549405533 0.852448741564063 9 0.3226139270646834 0.669429474481568 >10 1.8234672207714595 8.173901750995945 >50 0.27193589753893604 4.45137339307198 >100 0.3436007048597044 17.625316459656165 >500 0.0705188523424346 11.467785795714239 >1k 0.04483072399352672 18.254333649999875 >5k 0.001729008638868799 2.4768670154682964 >10k+ 7.410037023723425E-4 1.9797965625382028 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 14204 0.40463339997458936 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 12680 0.361218777223162 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 11045 0.31464206580676846 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 10798 0.307605706345087 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 10490 0.2988316224819376 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 10313 0.29378937298915375 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 8906 0.2537077626143123 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8815 0.2511154196547455 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8371 0.23846706499488085 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 6448 0.18368601542073726 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 6349 0.18086577417901067 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 6240 0.17776066008458444 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 5618 0.16004156864666594 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 5580 0.15895905180640724 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 5204 0.14824783254490023 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 5199 0.1481053961185504 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 5085 0.14485784559777434 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 5061 0.14417415075129517 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 5058 0.1440886888954853 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 5053 0.14394625246913545 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4918 0.14010046895769013 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 4916 0.1400434943871502 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 4796 0.13662502015475433 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 4759 0.1355709905997656 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 4689 0.133576880630868 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 4652 0.1325228510758793 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 4607 0.13124092323873088 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 4601 0.13106999952711107 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 4576 0.13035781739536192 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 4509 0.12844916928227423 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 4442 0.12654052116918654 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 4219 0.12018785655398426 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 4187 0.11927626342534536 No Hit GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT 4095 0.11665543318050853 No Hit ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG 4000 0.11394914107986183 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 3923 0.11175562011407449 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 3894 0.1109294888412455 No Hit ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC 3846 0.10956209914828716 No Hit ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT 3760 0.10711219261507013 No Hit GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG 3759 0.10708370532980015 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 3715 0.10583026477792166 No Hit GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA 3666 0.10443438779969337 No Hit ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA 3666 0.10443438779969337 No Hit GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC 3624 0.10323792181835482 No Hit ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT 3589 0.10224086683390601 No Hit GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG 3563 0.10150019741688693 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 5.697457053993091E-5 6 0.0 0.0 0.0 0.0 5.697457053993091E-5 7 0.0 0.0 0.0 2.8487285269965456E-5 5.697457053993091E-5 8 0.0 0.0 0.0 2.8487285269965456E-5 5.697457053993091E-5 9 0.0 0.0 0.0 2.8487285269965456E-5 5.697457053993091E-5 10-11 0.0 0.0 0.0 2.8487285269965456E-5 5.697457053993091E-5 12-13 0.0 0.0 0.0 2.8487285269965456E-5 1.994109968897582E-4 14-15 0.0 0.0 0.0 2.8487285269965456E-5 2.563855674296891E-4 16-17 0.0 0.0 0.0 4.273092790494818E-5 2.9911649533463733E-4 18-19 0.0 0.0 0.0 5.697457053993091E-5 3.1336013796962004E-4 20-21 0.0 0.0 0.0 8.546185580989637E-5 3.845783511445337E-4 22-23 0.0 0.0 0.0 1.5668006898481002E-4 3.988219937795164E-4 24-25 0.0 0.0 0.0 3.988219937795164E-4 3.988219937795164E-4 26-27 0.0 0.0 0.0 7.691567022890673E-4 4.7004020695443007E-4 28-29 0.0 0.0 0.0 0.0017092371161979273 4.842838495894128E-4 30-31 0.0 0.0 0.0 0.005668969768723126 4.842838495894128E-4 32-33 0.0 0.0 0.0 0.01660808731238986 4.985274922243955E-4 34-35 0.0 0.0 0.0 0.03495389902624761 5.412584201293437E-4 36-37 0.0 0.0 0.0 0.06616172003949478 5.412584201293437E-4 38-39 0.0 0.0 0.0 0.11769521909286229 5.412584201293437E-4 40-41 0.0 0.0 0.0 0.17549592090562222 5.412584201293437E-4 42-43 0.0 0.0 0.0 0.23385212478114642 5.412584201293437E-4 44-45 0.0 0.0 0.0 0.3063665094358435 5.555020627643264E-4 46-47 0.0 0.0 0.0 0.399932997905045 5.839893480342919E-4 48-49 0.0 0.0 0.0 0.5144803719755762 5.982329906692746E-4 50-51 0.0 0.0 0.0 0.6386422048247206 6.267202759392401E-4 52-53 0.0 0.0 0.0 0.7600977455732183 6.267202759392401E-4 54-55 0.0 0.0 0.0 0.893218829639767 6.267202759392401E-4 56-57 0.0 0.0 0.0 1.0566503852335587 6.409639185742228E-4 58-59 0.0 0.0 0.0 1.2602347694153668 6.552075612092055E-4 60-61 0.0 0.0 0.0 1.4769090611787241 6.552075612092055E-4 62-63 0.0 0.0 0.0 1.7017876910998315 6.836948464791709E-4 64-65 0.0 0.0 0.0 1.935454648526723 6.836948464791709E-4 66-67 0.0 0.0 0.0 2.20172530394509 6.979384891141536E-4 68-69 0.0 0.0 0.0 2.509687101356052 7.121821317491365E-4 70-71 0.0 0.0 0.0 2.8527737215048807 7.121821317491365E-4 72-73 0.0 0.0 0.0 3.213807331373788 7.406694170191019E-4 74-75 0.0 0.0 0.0 3.565169507893542 7.691567022890673E-4 76-77 0.0 0.0 0.0 3.97079996285258 7.691567022890673E-4 78-79 0.0 0.0 0.0 4.416326860832205 7.834003449240501E-4 80-81 0.0 0.0 0.0 4.907490389814314 8.118876301940155E-4 82-83 0.0 0.0 0.0 5.411316517098923 9.115931286388946E-4 84-85 0.0 0.0 0.0 5.898491826143237 9.258367712738773E-4 86-87 0.0 0.0 0.0 6.422956991605936 9.685676991788256E-4 88 0.0 0.0 0.0 6.843073231124752 9.97054984448791E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8340 0.0 45.727898 1 TACCCCG 310 0.0 37.901573 5 GTATCAA 13965 0.0 37.276325 1 GGGCACC 3425 0.0 36.810165 94 GTACTAG 3810 0.0 33.448048 1 TATTCTC 2150 0.0 32.57067 5 GTGGTAT 2270 0.0 31.280777 1 CAACGCA 16535 0.0 30.3561 5 ATCAACG 16720 0.0 30.088867 3 TAGGCAT 4715 0.0 29.604223 5 TATCAAC 18395 0.0 28.218493 2 AGGGCTA 4885 0.0 27.515688 5 CTGTGCG 4925 0.0 27.488937 9 AACGCAG 18790 0.0 27.262535 6 CTAGGCA 5340 0.0 27.117474 4 TACCTGG 1365 0.0 26.522997 2 TAGTACT 5150 0.0 26.47467 4 CTATTGA 5075 0.0 26.398579 9 GCTATTG 5075 0.0 25.929165 8 TCAACGC 20345 0.0 25.350628 4 >>END_MODULE