FastQCFastQC Report
Mon 16 Jan 2017
ERR1146477_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146477_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262508
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN100530.3081371754490717No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC84440.2588192887189855No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT72980.22369293807095644No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA67650.20735581338038098No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC60900.18666620894109684No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA58410.1790340437479387No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC55690.17069689944055308No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC49990.15322567791404648No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT39210.12018361334286383No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG35500.10881199371771655No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT34640.10617598485582258No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC33680.10323346333556885No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA41000.032.682021
GTATCAA60950.031.7383651
GTACTAG24700.031.3271791
GGGCACC30450.031.32383794
CAACGCA57750.031.0775325
ATCAACG60550.029.718933
CTGTGCG37700.027.6485449
TAGTACT37800.027.3442524
AACGCAG70650.026.9799396
GTACATG125400.026.3751471
TATCAAC75400.025.6736642
ACATGGG124000.025.310653
TACATGG128150.025.2983632
TAGGCAT33100.024.9815735
CTAGTAC41100.024.920873
GCATCGT28450.024.9204148
ACGCAGA71900.024.8150587
GTACTGT42600.024.4664236
ACTGTGC45800.024.3990028
CGCAGAG72200.024.3867918
Produced by FastQC (version 0.11.5)