##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146477_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3262508
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.405182761237672	31.0	30.0	34.0	26.0	34.0
2	30.44838112274361	31.0	30.0	34.0	26.0	34.0
3	30.713673652294492	31.0	31.0	34.0	27.0	34.0
4	34.190941447499895	37.0	35.0	37.0	30.0	37.0
5	34.38757575460351	37.0	35.0	37.0	30.0	37.0
6	34.50716228128789	37.0	35.0	37.0	30.0	37.0
7	34.444234619501316	37.0	35.0	37.0	30.0	37.0
8	34.35620571658368	37.0	35.0	37.0	30.0	37.0
9	35.74011067559068	39.0	35.0	39.0	30.0	39.0
10-11	35.66956188306665	39.0	35.0	39.0	29.5	39.0
12-13	35.662054008756456	39.0	35.0	39.0	30.0	39.0
14-15	36.68726743352047	40.0	36.0	41.0	29.0	41.0
16-17	36.51750647048222	40.0	36.0	41.0	28.0	41.0
18-19	36.468035633935614	40.0	36.0	41.0	27.5	41.0
20-21	36.80253688266818	40.0	36.0	41.0	30.0	41.0
22-23	36.53352589480241	40.0	36.0	41.0	28.0	41.0
24-25	36.651521927302554	40.0	36.0	41.0	29.0	41.0
26-27	36.7281264904178	40.0	36.0	41.0	29.5	41.0
28-29	36.56649746146216	39.5	35.5	41.0	29.0	41.0
30-31	36.51080365166921	39.0	35.0	41.0	29.0	41.0
32-33	36.610918042193305	39.5	35.0	41.0	29.5	41.0
34-35	36.658097236849684	40.0	35.0	41.0	30.0	41.0
36-37	36.58776468900612	39.5	35.0	41.0	29.5	41.0
38-39	36.42588477943962	39.0	35.0	41.0	29.0	41.0
40-41	36.39282509039059	39.0	35.0	41.0	29.0	41.0
42-43	36.236131528259854	39.0	35.0	41.0	28.0	41.0
44-45	36.066864050601566	39.0	35.0	41.0	28.0	41.0
46-47	35.94377193864352	39.0	34.5	41.0	27.5	41.0
48-49	35.83295136747557	39.0	34.0	40.0	27.5	41.0
50-51	35.63203369922771	38.0	34.0	40.0	27.0	41.0
52-53	35.46062936857166	38.0	34.0	40.0	27.0	41.0
54-55	35.27624805824231	38.0	34.0	40.0	27.0	41.0
56-57	35.140375134712315	37.5	33.0	40.0	27.0	41.0
58-59	34.91019439645818	37.0	33.0	40.0	26.0	41.0
60-61	34.661392554439715	36.5	33.0	40.0	26.0	41.0
62-63	34.40405847280681	36.0	33.0	39.5	26.0	41.0
64-65	34.12638788931705	35.5	32.5	39.0	26.0	41.0
66-67	33.835297568618984	35.0	32.0	39.0	26.0	41.0
68-69	33.55215113648764	35.0	32.0	38.5	25.0	40.0
70-71	33.45469451722417	35.0	32.0	38.0	25.5	40.0
72-73	33.230307787751016	35.0	32.0	37.0	25.0	40.0
74-75	32.96229695068947	35.0	32.0	37.0	25.0	39.5
76-77	32.71518889762109	35.0	32.0	36.0	25.0	39.0
78-79	32.491393124553255	35.0	31.0	36.0	25.0	39.0
80-81	32.249108354676835	35.0	31.0	36.0	24.0	38.0
82-83	32.016247163225344	35.0	31.0	35.0	24.0	37.0
84-85	31.820681052736113	35.0	31.0	35.0	24.0	37.0
86-87	31.642309076330235	34.5	31.0	35.0	24.0	36.0
88-89	31.626385283959458	35.0	31.0	35.0	24.0	36.0
90-91	31.548196663425806	35.0	31.0	35.0	24.0	36.0
92-93	31.41479607099814	35.0	31.0	35.0	23.0	36.0
94-95	31.381196000132412	35.0	31.0	35.0	23.0	35.0
96-97	31.28859408160838	34.5	31.0	35.0	23.0	35.0
98-99	31.211058946062355	34.0	31.0	35.0	23.0	35.0
100	30.725634695761666	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.7599513495112511
1101	2	0.974876620342414
1101	3	0.9621953218244741
1101	4	0.9904222952312267
1101	5	0.9189559138609553
1101	6	0.700665976417703
1101	7	0.7360213250241188
1101	8	0.7449210207522796
1101	9	0.99427480828043
1101	10-11	1.0056955269553498
1101	12-13	0.7589024520538601
1101	14-15	1.05881332779132
1101	16-17	1.1900631841789462
1101	18-19	1.3038151329359877
1101	20-21	0.9789731065412397
1101	22-23	1.3019243661246875
1101	24-25	1.1636152556602823
1101	26-27	1.0213713474115806
1101	28-29	1.2244837255987946
1101	30-31	1.2351345529576605
1101	32-33	1.0590581258909566
1101	34-35	0.9713584177197987
1101	36-37	1.0036798042156434
1101	38-39	1.0404815901229654
1101	40-41	0.9475105732664844
1101	42-43	1.0297879380256774
1101	44-45	1.0146209179129144
1101	46-47	1.0040342220521694
1101	48-49	0.9561191485098135
1101	50-51	1.0222405640063386
1101	52-53	1.0851067834763128
1101	54-55	0.971857966368475
1101	56-57	0.8960423324578102
1101	58-59	0.7887061982687058
1101	60-61	0.871463450659796
1101	62-63	0.8178160290599905
1101	64-65	0.8352465854310154
1101	66-67	0.8622907157022084
1101	68-69	0.8396572936028477
1101	70-71	0.7571503320394299
1101	72-73	0.7260638044183594
1101	74-75	0.7719077279434501
1101	76-77	0.734973456328575
1101	78-79	0.7564571371556212
1101	80-81	0.7566385583235018
1101	82-83	0.747129935560146
1101	84-85	0.7519093611958638
1101	86-87	0.757539995428246
1101	88-89	0.6671010748028792
1101	90-91	0.8060891672911907
1101	92-93	0.8417033302439982
1101	94-95	0.7776373087304265
1101	96-97	0.823208123393325
1101	98-99	0.7578673817326838
1101	100	0.836690820588526
1102	1	0.7916021164085869
1102	2	0.9331500357224201
1102	3	0.9332906807749914
1102	4	0.9151089694574139
1102	5	0.7961820599570828
1102	6	0.6173028415103801
1102	7	0.7228789882994917
1102	8	0.6851979581746406
1102	9	0.8432747975770525
1102	10-11	0.8600653086630174
1102	12-13	0.7913102471382274
1102	14-15	1.0457075871896748
1102	16-17	1.1983836078691823
1102	18-19	1.1960023250169982
1102	20-21	0.9869026206769504
1102	22-23	1.266978531534754
1102	24-25	1.1503332240576043
1102	26-27	1.0372193968461048
1102	28-29	1.1634244323242413
1102	30-31	1.2256579278851731
1102	32-33	1.0843907885839457
1102	34-35	0.9700232101402975
1102	36-37	0.9181476603848537
1102	38-39	0.9866117822864595
1102	40-41	0.9084813983650335
1102	42-43	0.9623785878028244
1102	44-45	0.9976679803696697
1102	46-47	1.026759548709947
1102	48-49	0.9407443302312046
1102	50-51	1.0109244525199017
1102	52-53	1.034266898518787
1102	54-55	0.9852301503558394
1102	56-57	0.795084765386953
1102	58-59	0.7677181643352071
1102	60-61	0.8161984648228824
1102	62-63	0.8690468629227155
1102	64-65	0.7756352000544879
1102	66-67	0.8912474963892834
1102	68-69	0.8732916097165386
1102	70-71	0.7394857769821783
1102	72-73	0.6458283001033251
1102	74-75	0.6879339493410228
1102	76-77	0.714134640135228
1102	78-79	0.7365381822212669
1102	80-81	0.7964963402804912
1102	82-83	0.7620446264559106
1102	84-85	0.7555864451916037
1102	86-87	0.7267057942814716
1102	88-89	0.74559865678291
1102	90-91	0.792428660945653
1102	92-93	0.8488876213936258
1102	94-95	0.7543033452616967
1102	96-97	0.7396965577592418
1102	98-99	0.7448364467544693
1102	100	0.8042048750737791
1103	1	0.5947020004266861
1103	2	0.8187359347657939
1103	3	0.7140649368588257
1103	4	0.7346303776667753
1103	5	0.7204883960663935
1103	6	0.4854411993708112
1103	7	0.5481996804847924
1103	8	0.5752150051788618
1103	9	0.6474030035636886
1103	10-11	0.7884343931949829
1103	12-13	0.6125415892008022
1103	14-15	0.8571145511066547
1103	16-17	1.1100102285229738
1103	18-19	1.051822697641832
1103	20-21	0.8169387853859078
1103	22-23	1.0466603041632183
1103	24-25	0.9530892319797744
1103	26-27	0.8417781611729467
1103	28-29	0.9614116131765726
1103	30-31	0.978520765816512
1103	32-33	0.8097829690595475
1103	34-35	0.8341179773365042
1103	36-37	0.7527682351221401
1103	38-39	0.813179221337009
1103	40-41	0.7545626203080786
1103	42-43	0.7925528999769469
1103	44-45	0.8497005202947037
1103	46-47	0.7892734947645721
1103	48-49	0.7498393078982488
1103	50-51	0.7897412731376079
1103	52-53	0.8772848817970456
1103	54-55	0.8452556131706359
1103	56-57	0.7017308789654848
1103	58-59	0.6781960941062621
1103	60-61	0.6011803994723337
1103	62-63	0.6203709020855612
1103	64-65	0.7107268062583287
1103	66-67	0.7487112230441113
1103	68-69	0.7837462252135268
1103	70-71	0.6344944780808675
1103	72-73	0.5490772745586625
1103	74-75	0.6308075730525715
1103	76-77	0.6099899667893709
1103	78-79	0.5955750744716042
1103	80-81	0.6476875447608847
1103	82-83	0.49808957864350845
1103	84-85	0.5592946919529069
1103	86-87	0.608462333255197
1103	88-89	0.6106235199258521
1103	90-91	0.6758971364573938
1103	92-93	0.7024851083491548
1103	94-95	0.6414678322641549
1103	96-97	0.6648884962047212
1103	98-99	0.6776253244022925
1103	100	0.7434907267404327
1104	1	0.5983082843015488
1104	2	0.708677640997017
1104	3	0.6872057711688235
1104	4	0.7093722053572051
1104	5	0.6724609524092173
1104	6	0.36469218446816853
1104	7	0.438014879598029
1104	8	0.508142500482478
1104	9	0.5676074696785278
1104	10-11	0.5934407162317754
1104	12-13	0.5146246353839814
1104	14-15	0.6991862615099507
1104	16-17	0.8228711709557786
1104	18-19	0.7677032293134047
1104	20-21	0.618845993245877
1104	22-23	0.8913273623976963
1104	24-25	0.7671862104115661
1104	26-27	0.6759199276854062
1104	28-29	0.8799507694293709
1104	30-31	0.9010967475840204
1104	32-33	0.7272412489600839
1104	34-35	0.679852422468862
1104	36-37	0.6326778457283595
1104	38-39	0.6816399957430122
1104	40-41	0.6592385212857437
1104	42-43	0.6682891671519471
1104	44-45	0.648497829144695
1104	46-47	0.5625890310368646
1104	48-49	0.49757700271360505
1104	50-51	0.6482068063448239
1104	52-53	0.7218086000843655
1104	54-55	0.6332362155671447
1104	56-57	0.5644834689987022
1104	58-59	0.4803107458104847
1104	60-61	0.5428485973917745
1104	62-63	0.4490623299276848
1104	64-65	0.49954489505233823
1104	66-67	0.5433459336442965
1104	68-69	0.5529053475130965
1104	70-71	0.4403421913400507
1104	72-73	0.42199235183391437
1104	74-75	0.36851336550159175
1104	76-77	0.46770865191002997
1104	78-79	0.4659901190861433
1104	80-81	0.4382541605953705
1104	82-83	0.4036471062963116
1104	84-85	0.4364481635238988
1104	86-87	0.35366764391036654
1104	88-89	0.34084042339493337
1104	90-91	0.4037441669723911
1104	92-93	0.3960869751041116
1104	94-95	0.30563217293402545
1104	96-97	0.4429096295801749
1104	98-99	0.4474929136188024
1104	100	0.5525696861214051
1105	1	0.48181201925216754
1105	2	0.6698930620066186
1105	3	0.6798424939866834
1105	4	0.7650228141712532
1105	5	0.6680143716842295
1105	6	0.38928558310226435
1105	7	0.4187139044386399
1105	8	0.4349600054795104
1105	9	0.4516780529401032
1105	10-11	0.5225153173241281
1105	12-13	0.47747928472139023
1105	14-15	0.6119602045173664
1105	16-17	0.772517648476132
1105	18-19	0.8479388436140809
1105	20-21	0.6054445070370633
1105	22-23	0.8270484814218477
1105	24-25	0.7066556469205167
1105	26-27	0.5102152607845696
1105	28-29	0.8082385906615457
1105	30-31	0.7656980653081717
1105	32-33	0.6517974612880622
1105	34-35	0.5708317706632471
1105	36-37	0.47189055295361726
1105	38-39	0.61518278214362
1105	40-41	0.5423621165992571
1105	42-43	0.5876290383326719
1105	44-45	0.5293941706649647
1105	46-47	0.4803847185133634
1105	48-49	0.4885879029253033
1105	50-51	0.5214732328224017
1105	52-53	0.5916484463965119
1105	54-55	0.5390695307671365
1105	56-57	0.5776888430999279
1105	58-59	0.5230100286626609
1105	60-61	0.4560188834737531
1105	62-63	0.3623531897900918
1105	64-65	0.3697488718499429
1105	66-67	0.4747733748844922
1105	68-69	0.5150004170052469
1105	70-71	0.4228993269142549
1105	72-73	0.3825829885007792
1105	74-75	0.34090395149871
1105	76-77	0.4018277677696531
1105	78-79	0.4208684806194185
1105	80-81	0.45934388645030566
1105	82-83	0.4460633147074944
1105	84-85	0.47920685898009197
1105	86-87	0.5046029747091794
1105	88-89	0.4276080047074302
1105	90-91	0.4307097566220861
1105	92-93	0.4879817980864125
1105	94-95	0.3662912759241621
1105	96-97	0.36839717041905473
1105	98-99	0.4396285344474862
1105	100	0.5063601247721614
1106	1	0.4691172389868896
1106	2	0.5177160396793425
1106	3	0.5536835984566544
1106	4	0.6602276439690584
1106	5	0.518561261974952
1106	6	0.27284913980872716
1106	7	0.28368507319550673
1106	8	0.2613274193998052
1106	9	0.44123331407739386
1106	10-11	0.4085135332498169
1106	12-13	0.20606775824631995
1106	14-15	0.4991210798429435
1106	16-17	0.5778762699964304
1106	18-19	0.5527878371552859
1106	20-21	0.33120537332934674
1106	22-23	0.7019662408654028
1106	24-25	0.5660632514431754
1106	26-27	0.42709621710861967
1106	28-29	0.6418211549108079
1106	30-31	0.6465303698504101
1106	32-33	0.4794711262434248
1106	34-35	0.4824980734891611
1106	36-37	0.3790844374178661
1106	38-39	0.5161683514665754
1106	40-41	0.45365165605214486
1106	42-43	0.39791814117665325
1106	44-45	0.44183790081055463
1106	46-47	0.46817416010562596
1106	48-49	0.4521112991403555
1106	50-51	0.502655389068174
1106	52-53	0.4798524782539033
1106	54-55	0.45830930433272954
1106	56-57	0.422454805672011
1106	58-59	0.48736655747132573
1106	60-61	0.41934994593894004
1106	62-63	0.4092901524976753
1106	64-65	0.40174835243590934
1106	66-67	0.4522755955677127
1106	68-69	0.33438095930362266
1106	70-71	0.3237347178297796
1106	72-73	0.2919905930234421
1106	74-75	0.3358453104062775
1106	76-77	0.3010781930869513
1106	78-79	0.3333250079225252
1106	80-81	0.317471210639944
1106	82-83	0.3218633252463263
1106	84-85	0.26723380076055747
1106	86-87	0.31704162363045185
1106	88-89	0.3928727553134195
1106	90-91	0.38549194147326205
1106	92-93	0.43549444468914444
1106	94-95	0.4395615895298022
1106	96-97	0.5501915200976164
1106	98-99	0.4610005402890138
1106	100	0.3780257124628008
1107	1	0.526389016848583
1107	2	0.6865449396852554
1107	3	0.6010454213100118
1107	4	0.716385567133905
1107	5	0.6602015659144342
1107	6	0.42128981215530814
1107	7	0.4085269059011054
1107	8	0.5230725720859795
1107	9	0.5565239860430893
1107	10-11	0.5861676259747952
1107	12-13	0.4153663192604
1107	14-15	0.6996034261565001
1107	16-17	0.8798994075291589
1107	18-19	0.9264948483166862
1107	20-21	0.6806557916324678
1107	22-23	0.9579042297819953
1107	24-25	0.6691087404332237
1107	26-27	0.584322506343014
1107	28-29	0.663597638161086
1107	30-31	0.7053127038668023
1107	32-33	0.670079955607747
1107	34-35	0.4589590328929205
1107	36-37	0.5522392369577602
1107	38-39	0.5241469442428865
1107	40-41	0.4813976691770989
1107	42-43	0.5742034941651397
1107	44-45	0.5145973773358534
1107	46-47	0.5447343130112543
1107	48-49	0.5500372498943804
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2214	1	-0.9026872647060529
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2214	3	-1.0655436390781539
2214	4	-0.882263965856481
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2214	6	-1.0204092071439277
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2214	9	-4.473720087469253
2214	10-11	-1.7156967735993263
2214	12-13	-1.335450401442145
2214	14-15	-3.7616004415331545
2214	16-17	-3.1231259419316757
2214	18-19	-1.722817040717878
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2214	22-23	-2.8516590305814233
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2214	32-33	-1.3940486206844724
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2214	86-87	-1.051502096072241
2214	88-89	-0.9783133105285344
2214	90-91	-0.9737432045650252
2214	92-93	-0.9797722704808969
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2214	96-97	-1.0651979173225392
2214	98-99	-1.308668687245241
2214	100	-1.1452742263343225
2215	1	-3.9496094968453015
2215	2	-2.131788821973352
2215	3	-3.659926025052499
2215	4	-1.8835875449551978
2215	5	-1.504658528109637
2215	6	-1.2406534699915142
2215	7	-1.2255220461226344
2215	8	-1.1728035555485263
2215	9	-1.6998446959123754
2215	10-11	-1.336899019618194
2215	12-13	-1.2423822010432133
2215	14-15	-3.6136670778787945
2215	16-17	-3.4032805977439082
2215	18-19	-2.300096227165298
2215	20-21	-1.7677569487159772
2215	22-23	-3.1849317387485456
2215	24-25	-5.382422931772247
2215	26-27	-3.608261845767686
2215	28-29	-1.7997407185586454
2215	30-31	-4.063040666302122
2215	32-33	-4.057467661368889
2215	34-35	-2.9379863517832376
2215	36-37	-1.8283518449500704
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2215	42-43	-1.8710057292786288
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2215	46-47	-2.735970231545039
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2215	50-51	-5.190301641982188
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2215	54-55	-3.135127162178385
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2215	64-65	-1.7546739721140838
2215	66-67	-1.4949841629472473
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2215	82-83	-1.446664286280253
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2215	86-87	-1.3279205782536607
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2215	90-91	-1.5375150111016787
2215	92-93	-3.7420078834344714
2215	94-95	-1.9135644943107408
2215	96-97	-1.5248251873835486
2215	98-99	-3.0732182732906104
2215	100	-2.9995355063620934
2216	1	-0.5926538078985004
2216	2	-0.5336859355645345
2216	3	-0.8144211291976049
2216	4	-0.7915791358557556
2216	5	-0.8926486343389328
2216	6	-0.7600679611523589
2216	7	-0.9464915053544516
2216	8	-0.7553471853635116
2216	9	-0.9441437915315021
2216	10-11	-0.9680588809823121
2216	12-13	-1.2180143178382181
2216	14-15	-1.587377981774324
2216	16-17	-1.2687758473278663
2216	18-19	-3.206406472878392
2216	20-21	-1.5169576283909478
2216	22-23	-0.9876059762564964
2216	24-25	-1.2087661561754004
2216	26-27	-1.046970212872381
2216	28-29	-1.064567747399984
2216	30-31	-1.1987968906472446
2216	32-33	-1.1799366303891077
2216	34-35	-1.4348259212678585
2216	36-37	-1.4807403569010589
2216	38-39	-1.4152711897651216
2216	40-41	-1.4100903013521062
2216	42-43	-1.4508404478822712
2216	44-45	-1.3529959705493226
2216	46-47	-1.5784654556751576
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2216	54-55	-1.385482758951163
2216	56-57	-1.3947404155171057
2216	58-59	-1.4769551494265372
2216	60-61	-1.4283049792473719
2216	62-63	-1.3404254565614195
2216	64-65	-1.3981099122731564
2216	66-67	-1.3839091250918187
2216	68-69	-1.4770705312561816
2216	70-71	-1.5227146520694177
2216	72-73	-1.5119804110652524
2216	74-75	-1.6095614461027665
2216	76-77	-1.4776527698616988
2216	78-79	-1.566695201627887
2216	80-81	-1.4277687691428333
2216	82-83	-1.5026755557044638
2216	84-85	-1.4883861415789497
2216	86-87	-1.388497825642041
2216	88-89	-1.3481794758286156
2216	90-91	-1.3967230789395337
2216	92-93	-1.3378975538628204
2216	94-95	-1.2406095819108458
2216	96-97	-1.2400474587947166
2216	98-99	-1.1810372036348937
2216	100	-1.1880244670455582
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	13448.0
1	2077.0
2	388.0
3	301.0
4	299.0
5	185.0
6	171.0
7	204.0
8	352.0
9	609.0
10	1140.0
11	2341.0
12	3858.0
13	5658.0
14	7534.0
15	9384.0
16	11424.0
17	13617.0
18	15656.0
19	17639.0
20	20257.0
21	22741.0
22	26387.0
23	30276.0
24	34239.0
25	38051.0
26	43693.0
27	49436.0
28	58680.0
29	70113.0
30	83411.0
31	99066.0
32	122177.0
33	154877.0
34	210094.0
35	283681.0
36	416186.0
37	633263.0
38	573121.0
39	186237.0
40	237.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.96756532846738	20.269518046402823	12.56276195317613	24.200154671953666
2	15.456112963664298	19.747118792279007	40.47700422135804	24.31976402269865
3	18.651547170345975	25.85939619961259	29.040779950158147	26.448276679883286
4	12.239574511331684	16.559750422686157	39.41005638895134	31.790618677030817
5	13.322847483842526	38.09124905678152	35.068331964877636	13.517571494498315
6	30.6478190431017	37.826835533488975	17.991493005291463	13.533852418117867
7	27.428242606160524	31.664313157226754	23.103977061292287	17.803467175320435
8	26.066430426602977	35.2680866742059	20.0234864067492	18.64199649244193
9	26.042808247139355	15.365918359864473	21.08416009819813	37.507113294798046
10-11	25.428344193892016	25.991416938527294	27.734622117576297	20.845616750004393
12-13	25.67878805339805	23.62629684643246	27.691144598945343	23.003770501224146
14-15	22.991620527881015	25.023483444037147	25.02781268646748	26.957083341614357
16-17	22.36817230847736	27.72076538337037	26.303293475586393	23.607768832565878
18-19	21.98333783725904	28.02476614765214	27.711822912831014	22.28007310225781
20-21	23.377174825235162	27.266365604671016	26.834401060367654	22.522058509726172
22-23	23.619170857974613	26.90667997740952	26.656025464958343	22.818123699657527
24-25	23.14239648482486	27.059499608286607	27.180611072894127	22.617492833994408
26-27	23.022484997832738	27.225186697231173	26.430893605144252	23.321434699791837
28-29	22.35745663186988	27.707634266634074	26.900695057728406	23.03421404376764
30-31	23.029150855066487	27.300524282212145	26.822764144482157	22.847560718239208
32-33	23.30668824195544	27.507399431584396	26.0433032929685	23.142609033491663
34-35	23.38072002921161	27.344301039816227	26.781900511049106	22.49307841992306
36-37	23.105506964356188	27.06155776164857	26.573654486754634	23.25928078724061
38-39	23.203463230143758	27.109535315647186	26.98946060523324	22.697540848975812
40-41	22.907953014384383	26.50742332294167	27.4765571165654	23.10806654610855
42-43	22.85066206961161	26.71042867497325	26.913265306122447	23.52564394929269
44-45	23.00276655886338	27.347433594034726	26.850013922942868	22.799785924159025
46-47	22.990896211136153	27.140009856094245	26.509780740427523	23.35931319234208
48-49	23.42689231083978	26.88332939978099	26.616859472353493	23.072918817025727
50-51	23.148273692241986	26.827628689601106	26.64441591626108	23.379681701895823
52-53	22.79104077273882	27.25937687332946	26.53374793148993	23.41583442244179
54-55	22.74896157832083	27.141179432548274	26.56669241855874	23.54316657057215
56-57	23.490595454808762	26.771556011304437	26.379699961669438	23.35814857221737
58-59	22.802768965766944	26.948899239042852	27.019741129741732	23.22859066544848
60-61	22.694959631915168	27.512384655736998	26.5163124653842	23.27634324696363
62-63	22.957346920698008	26.963740068476007	26.297697311411767	23.78121569941422
64-65	22.404293623580728	27.098037070789786	26.586740271055405	23.910929034574085
66-67	22.93530433576884	26.7956445878959	26.51503094776818	23.75402012856708
68-69	22.52170344686348	26.82192687251701	26.78102945307638	23.87534022754313
70-71	22.52865423877508	26.554476372107054	26.89490217938768	24.021967209730185
72-73	22.913205309802965	26.641062576915118	27.149836774698237	23.295895338583684
74-75	23.028052393392954	26.862830655792568	26.87928602199842	23.22983092881606
76-77	22.822260493292145	26.821737933482385	26.616103253628072	23.739898319597394
78-79	22.649752899990492	26.700269822823692	26.778046725626297	23.871930551559522
80-81	22.36220346276394	26.981959295368412	27.205155182537013	23.450682059330635
82-83	22.9641715139232	26.272893032198724	27.940287653958652	22.822647799919423
84-85	23.142067077057433	26.702861544193222	26.872393103672486	23.282678275076858
86-87	22.176864713420844	27.232623899514152	27.193661399336417	23.39684998772859
88-89	22.954192590498835	26.172335236157725	27.095249230633815	23.77822294270963
90-91	22.624449673852393	26.254119165938526	27.318784202422403	23.802646957786674
92-93	22.78231348383028	26.43666530827909	27.312530892987468	23.468490314903164
94-95	21.963253745580968	26.319324975694748	27.808585262386693	23.90883601633759
96-97	22.217980116575955	26.220169208366272	27.4539660089578	24.107884666099974
98-99	21.767662220572287	26.750271028050417	27.314684909458737	24.167381841918562
100	22.394340316629673	26.25220122726457	26.775878732402962	24.57757972370279
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	11326.0
1	6645.0
2	1925.5
3	1479.5
4	848.0
5	527.5
6	409.5
7	378.5
8	384.5
9	380.0
10	372.0
11	357.0
12	350.5
13	378.5
14	401.0
15	489.5
16	553.0
17	645.5
18	830.0
19	1062.5
20	1372.5
21	1774.5
22	2468.5
23	3489.5
24	4590.0
25	5938.5
26	8102.0
27	10898.0
28	14696.5
29	20841.0
30	29158.5
31	37404.5
32	48405.0
33	57757.5
34	63772.0
35	72116.0
36	80794.0
37	94114.5
38	100522.0
39	96030.0
40	92082.0
41	88495.5
42	88433.0
43	94161.0
44	101963.0
45	116561.5
46	131071.0
47	138903.0
48	143898.0
49	142217.5
50	137849.0
51	140381.0
52	140780.0
53	135315.0
54	128779.0
55	114417.0
56	101866.0
57	87560.5
58	72583.0
59	66444.0
60	55765.0
61	42061.5
62	32900.0
63	26486.5
64	19376.0
65	11857.0
66	7963.0
67	5901.5
68	4444.5
69	3312.0
70	2512.0
71	1951.0
72	1492.0
73	1160.0
74	925.5
75	722.5
76	540.0
77	363.5
78	218.0
79	130.5
80	71.5
81	45.0
82	50.0
83	38.5
84	24.5
85	18.5
86	12.0
87	12.5
88	13.5
89	9.0
90	5.0
91	6.5
92	7.0
93	3.0
94	4.5
95	4.5
96	1.0
97	0.5
98	2.5
99	3.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9945109713140934
2	0.45173835589062156
3	0.4854853995760317
4	0.4725812166590856
5	0.4389567780370193
6	0.4833091597016774
7	0.499370422999729
8	0.5019144780641152
9	0.5157075476903046
10-11	0.5296232223798378
12-13	0.526696026492502
14-15	0.5252554169982112
16-17	0.5289948714302003
18-19	0.5269259109862718
20-21	0.5277688207967612
22-23	0.4906501378693937
24-25	0.5256845347199148
26-27	0.5050409071793847
28-29	0.5107267169919583
30-31	0.5130102362967386
32-33	0.5155389657282067
34-35	0.5279067514930231
36-37	0.5458224163741514
38-39	0.5347113325086099
40-41	0.5394622787131863
42-43	0.5373779926363399
44-45	0.5475388872609661
46-47	0.5460523008679213
48-49	0.5188033255397381
50-51	0.5325350926342556
52-53	0.5246423916814916
54-55	0.5345274249135941
56-57	0.522787990098415
58-59	0.5288262894681025
60-61	0.522925920794677
62-63	0.516335898639942
64-65	0.528136635986793
66-67	0.5247649967448356
68-69	0.5457304625766435
70-71	0.5304967834561631
72-73	0.5349718682682157
74-75	0.5322898825075678
76-77	0.5194316764893757
78-79	0.5508492239712516
80-81	0.5257458372515869
82-83	0.5246117404156557
84-85	0.5330101872547132
86-87	0.8381895155506133
88-89	1.1030930805380401
90-91	1.0933766292680356
92-93	1.09250306819171
94-95	1.0985107162955616
96-97	1.0911544124949273
98-99	1.086388140657433
100	1.0829398732508855
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3262508.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.122436136834864
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.25294943643893	33.313353784549705
2	12.43137868147029	10.72142666569026
3	4.3732416640395355	5.657545031054709
4	1.9831762458276347	3.420775640351603
5	1.0850195381858774	2.339434287131928
6	0.6422216866265212	1.6616498200345522
7	0.39679191239898465	1.1977443731426454
8	0.2833835443189638	0.9776151033699542
9	0.19985727256308666	0.7756499225325333
>10	1.0602975707940394	8.559833860150414
>50	0.11912157722789707	3.6318050003993485
>100	0.14094623272757154	13.076109168566925
>500	0.02159741519949131	6.257609104739788
>1k	0.00951991327867252	6.875812124067146
>5k	4.26264773671904E-4	1.225652817050537
>10k+	7.104412894531732E-5	0.30798329716822176
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	10053	0.3081371754490717	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	8444	0.2588192887189855	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	7298	0.22369293807095644	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	6765	0.20735581338038098	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	6090	0.18666620894109684	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	5841	0.1790340437479387	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	5569	0.17069689944055308	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	4999	0.15322567791404648	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	3921	0.12018361334286383	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	3550	0.10881199371771655	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	3464	0.10617598485582258	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	3368	0.10323346333556885	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.06512658359765E-5
2	0.0	0.0	0.0	0.0	3.06512658359765E-5
3	0.0	0.0	0.0	0.0	3.06512658359765E-5
4	0.0	0.0	0.0	0.0	3.06512658359765E-5
5	0.0	0.0	0.0	0.0	3.06512658359765E-5
6	0.0	0.0	0.0	0.0	3.06512658359765E-5
7	0.0	0.0	0.0	0.0	3.06512658359765E-5
8	0.0	0.0	0.0	0.0	3.06512658359765E-5
9	0.0	0.0	0.0	0.0	3.06512658359765E-5
10-11	0.0	0.0	0.0	1.532563291798825E-5	3.06512658359765E-5
12-13	0.0	0.0	0.0	3.06512658359765E-5	7.662816458994123E-5
14-15	0.0	0.0	0.0	3.06512658359765E-5	1.2260506334390596E-4
16-17	0.0	0.0	0.0	3.06512658359765E-5	1.5325632917988246E-4
18-19	0.0	0.0	0.0	4.5976898753964746E-5	1.8390759501585896E-4
20-21	0.0	0.0	0.0	6.1302531671953E-5	2.6053575960580024E-4
22-23	0.0	0.0	0.0	9.195379750792948E-5	2.7586139252378845E-4
24-25	0.0	0.0	0.0	2.1455886085183546E-4	2.7586139252378845E-4
26-27	0.0	0.0	0.0	3.524895571137297E-4	3.831408229497062E-4
28-29	0.0	0.0	0.0	0.00148658639304486	3.9846645586769444E-4
30-31	0.0	0.0	0.0	0.00594634557217944	4.1379208878568265E-4
32-33	0.0	0.0	0.0	0.016153217095559613	4.291177217036709E-4
34-35	6.1302531671953E-5	0.0	0.0	0.03250566741905307	5.210715192116004E-4
36-37	6.1302531671953E-5	0.0	0.0	0.06015310920310386	5.210715192116004E-4
38-39	6.1302531671953E-5	0.0	0.0	0.1064058693495924	5.210715192116004E-4
40-41	6.1302531671953E-5	0.0	0.0	0.15874290576452227	5.210715192116004E-4
42-43	6.1302531671953E-5	0.0	0.0	0.21278108743334884	5.67048417965565E-4
44-45	6.1302531671953E-5	0.0	0.0	0.28157785360219806	6.436765825555064E-4
46-47	6.1302531671953E-5	0.0	0.0	0.3697155685135485	6.896534813094712E-4
48-49	6.1302531671953E-5	0.0	0.0	0.47434366444465426	7.203047471454477E-4
50-51	6.1302531671953E-5	0.0	0.0	0.5899295879121216	7.356303800634358E-4
52-53	6.1302531671953E-5	0.0	0.0	0.7019906158084517	7.969329117353889E-4
54-55	6.1302531671953E-5	1.532563291798825E-5	0.0	0.8262355218745824	7.969329117353889E-4
56-57	6.1302531671953E-5	3.06512658359765E-5	0.0	0.9841355178286153	7.969329117353889E-4
58-59	6.1302531671953E-5	3.06512658359765E-5	0.0	1.1727634077832145	7.969329117353889E-4
60-61	6.1302531671953E-5	3.06512658359765E-5	0.0	1.375797392680723	8.582354434073418E-4
62-63	6.1302531671953E-5	3.06512658359765E-5	0.0	1.5861110532142757	8.888867092433183E-4
64-65	6.1302531671953E-5	3.06512658359765E-5	0.0	1.804746532422296	9.042123421613066E-4
66-67	6.1302531671953E-5	3.06512658359765E-5	0.0	2.0569757989865467	9.501892409152713E-4
68-69	6.1302531671953E-5	3.06512658359765E-5	0.0	2.3462164690477385	9.501892409152713E-4
70-71	6.1302531671953E-5	3.06512658359765E-5	0.0	2.6698785106427323	9.501892409152713E-4
72-73	6.1302531671953E-5	3.06512658359765E-5	0.0	3.0090194414848943	0.001057468671341189
74-75	6.1302531671953E-5	3.06512658359765E-5	0.0	3.3418155602990094	0.0012107250005210716
76-77	6.1302531671953E-5	3.06512658359765E-5	0.0	3.71669586710592	0.0012567018992750362
78-79	6.1302531671953E-5	3.06512658359765E-5	0.0	4.135407484058277	0.001302678798029001
80-81	6.1302531671953E-5	3.06512658359765E-5	0.0	4.593046208622324	0.0013180044309469893
82-83	6.1302531671953E-5	3.06512658359765E-5	0.0	5.071144653131885	0.0013486556967829658
84-85	6.1302531671953E-5	3.06512658359765E-5	1.532563291798825E-5	5.536446194154927	0.001363981329700954
86-87	6.1302531671953E-5	3.06512658359765E-5	3.06512658359765E-5	6.0289200823415605	0.0013946325955369306
88	6.1302531671953E-5	3.06512658359765E-5	3.06512658359765E-5	6.432566602135535	0.0014406094942908951
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	4100	0.0	32.68202	1
GTATCAA	6095	0.0	31.738365	1
GTACTAG	2470	0.0	31.327179	1
GGGCACC	3045	0.0	31.323837	94
CAACGCA	5775	0.0	31.077532	5
ATCAACG	6055	0.0	29.71893	3
CTGTGCG	3770	0.0	27.648544	9
TAGTACT	3780	0.0	27.344252	4
AACGCAG	7065	0.0	26.979939	6
GTACATG	12540	0.0	26.375147	1
TATCAAC	7540	0.0	25.673664	2
ACATGGG	12400	0.0	25.31065	3
TACATGG	12815	0.0	25.298363	2
TAGGCAT	3310	0.0	24.981573	5
CTAGTAC	4110	0.0	24.92087	3
GCATCGT	2845	0.0	24.920414	8
ACGCAGA	7190	0.0	24.815058	7
GTACTGT	4260	0.0	24.466423	6
ACTGTGC	4580	0.0	24.399002	8
CGCAGAG	7220	0.0	24.386791	8
>>END_MODULE