FastQCFastQC Report
Mon 16 Jan 2017
ERR1146477_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146477_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3262508
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC94910.2909111640492529No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73760.22608373680616262No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA73710.22593048047698272No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70670.2166124956628459No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT69990.2145282095859995No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA63880.19580028616021783No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC61940.1898539405880384No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC61530.18859723868876338No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC52540.16104175070222052No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41210.12631386651005913No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC38890.11920277283611258No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT38300.11739434815178998No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG37830.11595373865749907No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT35610.1091491576419123No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG35240.10801506080598117No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG32650.10007638295446326No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA63150.047.35311
GTATCAA107150.037.912831
GGGCACC28800.034.62697294
GTACTAG26800.033.5091361
GTGGTAT18800.033.2627751
CAACGCA126850.030.8260425
ATCAACG129650.030.133293
TATCAAC139600.029.0313032
AACGCAG143400.027.8246776
TAGTACT39100.027.653444
TCAACGC146350.026.9184064
CTGTGCG38500.026.8604589
CTAACGC12350.025.8857043
CTAGTAC43050.025.4449043
ACGCAGA153900.025.3460987
CGCAGAG155800.024.9461178
GCATCGT33800.024.8886458
CGCCTAC12600.024.6175587
GTACATG120450.024.4751511
TCTAACG13300.024.3917032
Produced by FastQC (version 0.11.5)