##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146477_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3262508 Sequences flagged as poor quality 0 Sequence length 100 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.830704782946125 31.0 31.0 34.0 30.0 34.0 2 31.790746566751714 31.0 31.0 34.0 30.0 34.0 3 31.965029970807734 31.0 31.0 34.0 30.0 34.0 4 35.71215702766093 37.0 35.0 37.0 33.0 37.0 5 35.629626348808955 37.0 35.0 37.0 33.0 37.0 6 35.670903795484946 37.0 35.0 37.0 33.0 37.0 7 35.58772790748712 37.0 35.0 37.0 33.0 37.0 8 35.569517683941314 37.0 35.0 37.0 33.0 37.0 9 37.160490947455145 39.0 37.0 39.0 33.0 39.0 10-11 37.108032072258524 39.0 37.0 39.0 33.0 39.0 12-13 37.05075619737944 39.0 37.0 39.0 33.0 39.0 14-15 38.25809944374083 40.0 38.0 41.0 33.0 41.0 16-17 38.258643503709415 40.0 37.5 41.0 33.0 41.0 18-19 38.315109572145104 40.0 38.0 41.0 33.0 41.0 20-21 38.33183780698775 40.0 38.0 41.0 33.0 41.0 22-23 38.29693521058033 40.0 38.0 41.0 33.0 41.0 24-25 38.238562786665966 40.0 37.0 41.0 33.0 41.0 26-27 38.100916227638365 40.0 37.0 41.0 33.0 41.0 28-29 38.028201156901375 40.0 37.0 41.0 33.0 41.0 30-31 37.89686707281637 40.0 37.0 41.0 33.0 41.0 32-33 37.7728341202535 40.0 36.5 41.0 33.0 41.0 34-35 37.674621027749204 40.0 36.0 41.0 32.0 41.0 36-37 37.586922698733616 40.0 36.0 41.0 32.0 41.0 38-39 37.476920363107155 40.0 36.0 41.0 32.0 41.0 40-41 37.3575125333026 40.0 36.0 41.0 31.0 41.0 42-43 37.229038978601736 39.5 36.0 41.0 31.0 41.0 44-45 37.050520642401494 39.0 35.0 41.0 31.0 41.0 46-47 36.81681071739901 39.0 35.0 41.0 30.0 41.0 48-49 36.576620655029814 39.0 35.0 41.0 30.0 41.0 50-51 36.38751966278703 39.0 35.0 41.0 30.0 41.0 52-53 36.21517066011792 39.0 35.0 41.0 29.0 41.0 54-55 36.03057954187392 38.0 35.0 40.0 29.0 41.0 56-57 35.79621628514015 38.0 34.5 40.0 28.5 41.0 58-59 35.54383391550304 37.5 34.0 40.0 28.0 41.0 60-61 35.29188510802119 37.0 34.0 40.0 28.0 41.0 62-63 35.0307758632316 36.5 34.0 40.0 28.0 41.0 64-65 34.76693773624463 36.0 34.0 39.5 27.0 41.0 66-67 34.54042549474208 36.0 33.5 39.0 27.0 41.0 68-69 34.340257556456564 35.0 33.0 39.0 27.0 41.0 70-71 34.100303202321655 35.0 33.0 38.5 26.5 40.5 72-73 33.87863738571676 35.0 33.0 37.5 26.0 40.0 74-75 33.6817155697396 35.0 33.0 37.0 26.5 39.5 76-77 33.50098681750359 35.0 33.0 36.5 27.0 39.0 78-79 33.35918670544256 35.0 33.0 36.0 27.0 39.0 80-81 33.188519231217214 35.0 33.0 36.0 27.0 38.0 82-83 32.9876942830485 35.0 33.0 35.5 27.0 37.0 84-85 32.828168850467186 35.0 33.0 35.0 27.0 37.0 86-87 32.680825150467065 35.0 33.0 35.0 26.0 36.5 88-89 32.55573534225817 35.0 33.0 35.0 26.0 36.0 90-91 32.459852818751706 35.0 33.0 35.0 26.0 36.0 92-93 32.3826738509147 35.0 33.0 35.0 26.0 36.0 94-95 32.32869298711298 35.0 33.0 35.0 26.0 35.0 96-97 32.27070355076524 35.0 33.0 35.0 26.0 35.0 98-99 32.227002201986934 35.0 33.0 35.0 25.5 35.0 100 31.736624400614495 34.0 32.0 35.0 25.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.49120860787474996 1101 2 0.6165166332724077 1101 3 0.524020864981523 1101 4 0.3755431535057028 1101 5 0.345504425103762 1101 6 0.40526435383159765 1101 7 0.4446101207158364 1101 8 0.45941131331500884 1101 9 0.4581737365472165 1101 10-11 0.5179726961563986 1101 12-13 0.5402349283801087 1101 14-15 0.7033106945646992 1101 16-17 0.6922569477668361 1101 18-19 0.6678755220886856 1101 20-21 0.649900650960646 1101 22-23 0.6849695806361282 1101 24-25 0.7233448286255708 1101 26-27 0.731326963994043 1101 28-29 0.7365307055055865 1101 30-31 0.7804271300899686 1101 32-33 0.7886002156109058 1101 34-35 0.7587049086137085 1101 36-37 0.7276634311581063 1101 38-39 0.7844035862964986 1101 40-41 0.8281726564390226 1101 42-43 0.8724802756236087 1101 44-45 0.7582544095076855 1101 46-47 0.73687985109256 1101 48-49 0.7986624505493936 1101 50-51 0.7054037121231573 1101 52-53 0.7279150429262486 1101 54-55 0.7794191802380084 1101 56-57 0.6792156987033806 1101 58-59 0.7049306865874883 1101 60-61 0.6898481749928607 1101 62-63 0.6479569372121361 1101 64-65 0.7026149406323299 1101 66-67 0.6696221765552579 1101 68-69 0.699667510615221 1101 70-71 0.6977559626337921 1101 72-73 0.6619954032366451 1101 74-75 0.6348655664034979 1101 76-77 0.616788319025261 1101 78-79 0.49714887573161803 1101 80-81 0.554981018732903 1101 82-83 0.5725748964209956 1101 84-85 0.5738445289341456 1101 86-87 0.5649615711189213 1101 88-89 0.5089536768824985 1101 90-91 0.5430507333203067 1101 92-93 0.5835531697208651 1101 94-95 0.551132343208856 1101 96-97 0.5015997509253296 1101 98-99 0.5349398467271698 1101 100 0.7208774754102052 1102 1 0.4900107734837782 1102 2 0.5605794199420409 1102 3 0.4696463454531994 1102 4 0.383389598377299 1102 5 0.31079634726120986 1102 6 0.35908240766709554 1102 7 0.35913023457464277 1102 8 0.4261184429638192 1102 9 0.49560850100669285 1102 10-11 0.5204057874253962 1102 12-13 0.4712676457862628 1102 14-15 0.6794921669471421 1102 16-17 0.6827781190568345 1102 18-19 0.6938156410725185 1102 20-21 0.6566928520356399 1102 22-23 0.6681417388724142 1102 24-25 0.6784501609246547 1102 26-27 0.6731542784587816 1102 28-29 0.71002259895279 1102 30-31 0.6567517523392681 1102 32-33 0.6734670812765344 1102 34-35 0.6479553995526643 1102 36-37 0.7193095200478083 1102 38-39 0.6994859818771246 1102 40-41 0.6972458159452302 1102 42-43 0.7731904292927041 1102 44-45 0.666233064759929 1102 46-47 0.7124680334253028 1102 48-49 0.7139042163430176 1102 50-51 0.6831045925707855 1102 52-53 0.6213007383596292 1102 54-55 0.6342802944097343 1102 56-57 0.6802006853210045 1102 58-59 0.6594286806477427 1102 60-61 0.6083211816925456 1102 62-63 0.6573632426375511 1102 64-65 0.6043303465140326 1102 66-67 0.5907219978717322 1102 68-69 0.5421243492264765 1102 70-71 0.5492314632215312 1102 72-73 0.5780311081456588 1102 74-75 0.5318745928175517 1102 76-77 0.4967490218722972 1102 78-79 0.4955139340996837 1102 80-81 0.5176601351120382 1102 82-83 0.5773746156775772 1102 84-85 0.5747988220292655 1102 86-87 0.6197686332104979 1102 88-89 0.5559290899543541 1102 90-91 0.5496497619568856 1102 92-93 0.5140720460798036 1102 94-95 0.5808327728552101 1102 96-97 0.5754475397010097 1102 98-99 0.5051644934053741 1102 100 0.5999398811653478 1103 1 0.27573763130834195 1103 2 -0.17561514791523436 1103 3 0.1826970941663646 1103 4 0.17865584852079763 1103 5 0.21376675959587033 1103 6 0.23323293867708372 1103 7 0.3013674390624388 1103 8 0.29689822872490623 1103 9 0.39133667606885325 1103 10-11 0.40626749017452113 1103 12-13 0.35993939871814007 1103 14-15 0.466424093121077 1103 16-17 0.47390294927262033 1103 18-19 0.4917895210748142 1103 20-21 0.4906408458631475 1103 22-23 0.47452772636644625 1103 24-25 0.39505563738340044 1103 26-27 0.4967001195739371 1103 28-29 0.5138094605393775 1103 30-31 0.5772888089231358 1103 32-33 0.5978813545396306 1103 34-35 0.533264229042345 1103 36-37 0.5305074868012767 1103 38-39 0.612808290871449 1103 40-41 0.5843477284941088 1103 42-43 0.580528013255261 1103 44-45 0.522266080946082 1103 46-47 0.5843001544793651 1103 48-49 0.6460463590510059 1103 50-51 0.5964944638374519 1103 52-53 0.614847993445828 1103 54-55 0.5575905782264385 1103 56-57 0.5941995428082123 1103 58-59 0.5826999121107193 1103 60-61 0.6996457060341186 1103 62-63 0.6226400321932388 1103 64-65 0.6181983720035973 1103 66-67 0.6228009653206996 1103 68-69 0.5932172029895995 1103 70-71 0.5354394263951008 1103 72-73 0.5777311021829519 1103 74-75 0.5482913181225797 1103 76-77 0.5366131620021974 1103 78-79 0.4880247997983531 1103 80-81 0.4709708829470429 1103 82-83 0.40304742141707806 1103 84-85 0.41451628627964254 1103 86-87 0.48411617323717593 1103 88-89 0.42194909851374973 1103 90-91 0.4547499540105804 1103 92-93 0.45133558284988595 1103 94-95 0.5014024958733145 1103 96-97 0.49173769532624334 1103 98-99 0.5167439413853288 1103 100 0.5564918610006586 1104 1 0.34130704746079843 1104 2 0.41502211941969946 1104 3 0.35127766574971986 1104 4 0.2638640549637117 1104 5 0.215650954214766 1104 6 0.2703609031972647 1104 7 0.31408911544060913 1104 8 0.2838191784350599 1104 9 0.2931874446701386 1104 10-11 0.3236709619194542 1104 12-13 0.34582564156959705 1104 14-15 0.38099740776448954 1104 16-17 0.3793219877705951 1104 18-19 0.45825781030510626 1104 20-21 0.40371429938223713 1104 22-23 0.423760203468575 1104 24-25 0.4526827797426023 1104 26-27 0.511438102190084 1104 28-29 0.5131703348291836 1104 30-31 0.5250836348631722 1104 32-33 0.5166941070755513 1104 34-35 0.5226627934088057 1104 36-37 0.4689631846888247 1104 38-39 0.5141579011189776 1104 40-41 0.5404312830248585 1104 42-43 0.473514084485295 1104 44-45 0.4718291463097941 1104 46-47 0.42565611211428234 1104 48-49 0.40624581667444915 1104 50-51 0.3736378743626716 1104 52-53 0.39049488622151074 1104 54-55 0.41963255617160655 1104 56-57 0.3771723688628228 1104 58-59 0.47040895903554514 1104 60-61 0.4091969393297106 1104 62-63 0.4444116791535748 1104 64-65 0.4311775230275856 1104 66-67 0.3005916261501298 1104 68-69 0.3425626165867044 1104 70-71 0.3080916602989987 1104 72-73 0.28693562944482665 1104 74-75 0.30077961998271263 1104 76-77 0.3080215445269445 1104 78-79 0.23853629749034866 1104 80-81 0.21225870530924595 1104 82-83 0.1657651359927499 1104 84-85 0.15248852444140937 1104 86-87 0.20507158065696274 1104 88-89 0.29779287548058164 1104 90-91 0.2094029267809816 1104 92-93 0.14835725819992263 1104 94-95 0.19980513962084245 1104 96-97 0.24988571228755774 1104 98-99 0.1816358815793535 1104 100 0.26927234985384274 1105 1 0.1441921919006468 1105 2 -0.33121075622804597 1105 3 0.021077100611119448 1105 4 0.05998362312073624 1105 5 0.12533159495385604 1105 6 0.25300169824593155 1105 7 0.2708560905813542 1105 8 0.20487797398731544 1105 9 0.2360214841676651 1105 10-11 0.3177581838187322 1105 12-13 0.3351746208696085 1105 14-15 0.3914392992069011 1105 16-17 0.45848153062382835 1105 18-19 0.4016942912759802 1105 20-21 0.38673270835233353 1105 22-23 0.36369095223058423 1105 24-25 0.37263080439980456 1105 26-27 0.3949759537308424 1105 28-29 0.350652071962017 1105 30-31 0.35482036203303835 1105 32-33 0.4236713771887963 1105 34-35 0.45117000218990455 1105 36-37 0.44627639052921353 1105 38-39 0.3917376282484284 1105 40-41 0.3995773268992622 1105 42-43 0.3391194324745186 1105 44-45 0.3388933865278929 1105 46-47 0.38228052031966797 1105 48-49 0.36350892008734803 1105 50-51 0.3680194781377182 1105 52-53 0.43440355945141107 1105 54-55 0.4322704602472527 1105 56-57 0.35297181615851514 1105 58-59 0.3284441931577291 1105 60-61 0.2741984081305162 1105 62-63 0.3229044330696027 1105 64-65 0.32333047415148997 1105 66-67 0.36421906676861227 1105 68-69 0.4153578999486882 1105 70-71 0.2890399538477766 1105 72-73 0.2506445535365813 1105 74-75 0.2755207924121521 1105 76-77 0.2919846877295811 1105 78-79 0.24306591597515848 1105 80-81 0.27166384875435057 1105 82-83 0.257474416016116 1105 84-85 0.22851820043483428 1105 86-87 0.24996609899910283 1105 88-89 0.207944698192442 1105 90-91 0.1590299757192497 1105 92-93 0.19237177834503427 1105 94-95 0.21241848596874036 1105 96-97 0.20386320932664148 1105 98-99 0.22825608543958253 1105 100 0.18559950852474216 1106 1 0.32037931645463047 1106 2 0.3502920817298296 1106 3 0.3061985230528208 1106 4 0.18573150406200511 1106 5 0.23907536574923682 1106 6 0.19341060506756946 1106 7 0.201684066673252 1106 8 0.2075627423587676 1106 9 0.290126737024444 1106 10-11 0.29332332887827306 1106 12-13 0.30238698593487356 1106 14-15 0.36227777649570925 1106 16-17 0.38765848520145596 1106 18-19 0.3283315746652633 1106 20-21 0.3155184086579581 1106 22-23 0.3310410749327417 1106 24-25 0.33457431624093203 1106 26-27 0.3503353280251176 1106 28-29 0.30973937115956574 1106 30-31 0.33472938929850926 1106 32-33 0.33218035778228483 1106 34-35 0.3066142517495294 1106 36-37 0.40313660793033534 1106 38-39 0.33293995883663996 1106 40-41 0.2319466076990082 1106 42-43 0.2895906689810417 1106 44-45 0.29378434721802904 1106 46-47 0.3171055380098622 1106 48-49 0.3179371129781501 1106 50-51 0.2911358494660661 1106 52-53 0.2430924530242251 1106 54-55 0.11804679604466628 1106 56-57 0.18585900545105716 1106 58-59 0.21407618454599486 1106 60-61 0.26479682579962116 1106 62-63 0.2824324814278043 1106 64-65 0.21147616602605268 1106 66-67 0.18110642167142998 1106 68-69 0.2164386951791144 1106 70-71 0.21550278186903427 1106 72-73 0.24675779332520165 1106 74-75 0.21419624896956435 1106 76-77 0.2549946920956714 1106 78-79 0.26933001166779036 1106 80-81 0.22869701597556258 1106 82-83 0.22899697665206276 1106 84-85 0.1958352010822182 1106 86-87 0.22215053615000357 1106 88-89 0.23426769844838446 1106 90-91 0.1614008373443312 1106 92-93 0.19448368572372488 1106 94-95 0.08939037738861799 1106 96-97 0.1347905515828529 1106 98-99 0.24054361498595256 1106 100 0.2785113888035724 1107 1 0.09385589180668319 1107 2 -0.4773934794035881 1107 3 -0.02146088393363854 1107 4 0.07228910684762013 1107 5 0.1036963138062319 1107 6 0.18269962943082874 1107 7 0.18757649804037868 1107 8 0.1995796696418921 1107 9 0.22038164382898628 1107 10-11 0.25842762080643666 1107 12-13 0.21243440438272643 1107 14-15 0.1911606425549195 1107 16-17 0.2752857236753954 1107 18-19 0.2381349179705481 1107 20-21 0.2532057363752287 1107 22-23 0.2220619862796127 1107 24-25 0.2684655382054828 1107 26-27 0.29670951442619753 1107 28-29 0.2772227110405012 1107 30-31 0.353969827164633 1107 32-33 0.32149705983503907 1107 34-35 0.33232085808391076 1107 36-37 0.36841940825956243 1107 38-39 0.4215830011624675 1107 40-41 0.3522139495126666 1107 42-43 0.35268324994554234 1107 44-45 0.37173563956299205 1107 46-47 0.40762834163284367 1107 48-49 0.38951404843037807 1107 50-51 0.3951030635560784 1107 52-53 0.3890447995155242 1107 54-55 0.2874485480460436 1107 56-57 0.3292286934114088 1107 58-59 0.39354488898708695 1107 60-61 0.40276727850578453 1107 62-63 0.34681368918349875 1107 64-65 0.30465074985407625 1107 66-67 0.32347447109805216 1107 68-69 0.3495754915246394 1107 70-71 0.3352829346803503 1107 72-73 0.3355909882700914 1107 74-75 0.3718688273440378 1107 76-77 0.35243189402999064 1107 78-79 0.296056484634164 1107 80-81 0.30431248181864845 1107 82-83 0.28955628725631755 1107 84-85 0.26411419291784455 1107 86-87 0.2844500043973781 1107 88-89 0.2668365398994794 1107 90-91 0.2927716120983632 1107 92-93 0.3193971974783949 1107 94-95 0.3253578795007215 1107 96-97 0.2551390583116486 1107 98-99 0.26985145872707506 1107 100 0.27973530131160373 1108 1 0.18458432161813576 1108 2 0.3600671613732267 1108 3 0.13738247304802087 1108 4 0.13168663179739326 1108 5 0.15191682005239926 1108 6 0.206506117463384 1108 7 0.19232040551709417 1108 8 0.22207177868310168 1108 9 0.24238330623544613 1108 10-11 0.22513774031035183 1108 12-13 0.20607891160153713 1108 14-15 0.2941855994150302 1108 16-17 0.210028062292551 1108 18-19 0.23741583235556618 1108 20-21 0.25474813594778567 1108 22-23 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-0.7836990985905175 2215 18-19 -0.791632275534262 2215 20-21 -0.7872675760183654 2215 22-23 -0.7650489843402823 2215 24-25 -0.750663975550296 2215 26-27 -0.7534703299297192 2215 28-29 -0.7362713543996264 2215 30-31 -0.812811324092408 2215 32-33 -0.8196053042533649 2215 34-35 -0.7609271794876307 2215 36-37 -0.8222372865767014 2215 38-39 -0.9610997325987043 2215 40-41 -0.9361509593094013 2215 42-43 -0.7946732050556662 2215 44-45 -0.7421686697453751 2215 46-47 -0.7829832174359197 2215 48-49 -0.7740589794414277 2215 50-51 -0.8308635434856981 2215 52-53 -0.8059919809091411 2215 54-55 -0.7879284546455736 2215 56-57 -0.8252877227111028 2215 58-59 -0.8317470622023322 2215 60-61 -0.7830266760962274 2215 62-63 -0.7962261838541522 2215 64-65 -0.831372446833619 2215 66-67 -0.8057645216682658 2215 68-69 -0.6490062544019466 2215 70-71 -0.7188104245901812 2215 72-73 -0.7608508306610844 2215 74-75 -0.7084393547215697 2215 76-77 -0.6503237878024919 2215 78-79 -0.5589427332318948 2215 80-81 -0.6057506110939457 2215 82-83 -0.6702992024162384 2215 84-85 -0.5756729938738943 2215 86-87 -0.5592488972976568 2215 88-89 -0.5008015483553478 2215 90-91 -0.5042836739135623 2215 92-93 -0.566336110290905 2215 94-95 -0.601868089003105 2215 96-97 -0.5371029200347124 2215 98-99 -0.5039757264837483 2215 100 -0.5483803798798412 2216 1 -0.345537871173601 2216 2 -0.1450741406335183 2216 3 -0.40137963652056285 2216 4 -0.2635411438646216 2216 5 -0.32555316584093674 2216 6 -0.35211372730782386 2216 7 -0.4065013764681211 2216 8 -0.411990438670486 2216 9 -0.4559924540720033 2216 10-11 -0.5386441916514855 2216 12-13 -0.5403474314280032 2216 14-15 -0.7727987168443491 2216 16-17 -0.7579257088593181 2216 18-19 -0.736195027162104 2216 20-21 -0.7092689308831979 2216 22-23 -0.7144246612509377 2216 24-25 -0.748836864127199 2216 26-27 -0.8295564377527072 2216 28-29 -0.7805746936904683 2216 30-31 -0.82360706942071 2216 32-33 -0.8494964059899317 2216 34-35 -0.7556497624424097 2216 36-37 -0.8873786704130779 2216 38-39 -0.9402344131525311 2216 40-41 -0.8350909253092809 2216 42-43 -0.9135556167183339 2216 44-45 -0.8787941306461775 2216 46-47 -0.8458778901588033 2216 48-49 -0.9535147303859404 2216 50-51 -0.9479150993090499 2216 52-53 -0.9771843986422724 2216 54-55 -0.8656669140069511 2216 56-57 -0.9191701814347439 2216 58-59 -1.011333049262781 2216 60-61 -0.927253649757553 2216 62-63 -0.9487671528828727 2216 64-65 -0.8742055400741648 2216 66-67 -0.8217966591537333 2216 68-69 -0.875061060542258 2216 70-71 -0.9020880786589913 2216 72-73 -0.9244606623276823 2216 74-75 -0.8836483852222301 2216 76-77 -0.8093015515356399 2216 78-79 -0.7484248645094098 2216 80-81 -0.7496954849390463 2216 82-83 -0.7181906745840152 2216 84-85 -0.6942460512809916 2216 86-87 -0.6459990206587776 2216 88-89 -0.6437104875086241 2216 90-91 -0.6255984010585678 2216 92-93 -0.6516184020340461 2216 94-95 -0.6996284738652534 2216 96-97 -0.6964276049533424 2216 98-99 -0.643695773466149 2216 100 -0.6037056640687553 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 5.0 13 44.0 14 211.0 15 618.0 16 1464.0 17 2811.0 18 4997.0 19 7921.0 20 11276.0 21 16242.0 22 22185.0 23 26460.0 24 28439.0 25 29829.0 26 32483.0 27 39807.0 28 49326.0 29 61715.0 30 77287.0 31 95117.0 32 118105.0 33 149624.0 34 200761.0 35 275242.0 36 408532.0 37 689948.0 38 668319.0 39 243065.0 40 674.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.567518726462595 20.882469701219613 13.828046086947193 24.721965485370596 2 15.031399135163568 20.377543679389497 39.599233804087916 24.991823381359026 3 18.260535059648607 25.633513566533022 29.952642807224038 26.15330856659433 4 12.467029653459928 17.12262807425583 38.03463469177957 32.37570758050467 5 12.791731291495271 38.29283170162027 35.0367212069568 13.87871579992766 6 30.42968783524822 38.40398245766753 17.344172029616477 13.822157677467764 7 26.455259827377407 32.360559854074786 23.139697157970915 18.044483160576895 8 26.241314963825374 35.22026612655049 19.585361936277245 18.953056973346886 9 25.854342732646174 14.850262436138086 21.033542293229626 38.261852537986115 10-11 25.43095679765383 26.14001253023747 27.349879295315137 21.079151376793558 12-13 25.90836558868208 23.703022337416492 27.92739512056369 22.46121695333774 14-15 22.98413826283715 24.853462586767943 25.760053386932803 26.402345763462108 16-17 21.859243380332718 27.52845779902241 26.926113250032145 23.68618557061272 18-19 21.45994737790681 27.039106111004173 29.508954460801323 21.991992050287692 20-21 22.663011602585804 26.248538125069825 29.085544836629396 22.002905435714975 22-23 22.854133754607687 25.98173721724748 28.835929209724377 22.328199818420455 24-25 22.427331978956065 26.08617358179658 29.44020367153123 22.046290767716126 26-27 22.364621497434023 26.229570504346206 28.686964387137355 22.718843611082416 28-29 21.774245457788915 26.719198849474086 29.088158557772122 22.41839713496488 30-31 22.498810730885562 26.336241934119396 28.854396678874046 22.310550656121 32-33 22.756986343021996 26.501406280076555 28.288988103630704 22.452619273270745 34-35 22.75105475402917 26.39728759363437 28.907593753912845 21.944063898423614 36-37 22.593063373331194 26.086709978948715 28.671960344618313 22.648266303101785 38-39 22.697032467046824 26.092840232115904 29.09303210904004 22.117095191797233 40-41 22.403053491269716 25.61731129089853 29.386727029466712 22.59290818836504 42-43 22.54659678738605 25.74342521579036 28.705233298497905 23.004744698325677 44-45 22.64014371765525 26.432180396186 28.490719409730183 22.436956476428563 46-47 22.77059550505317 26.33676300563861 27.83056777178784 23.062073717520388 48-49 23.367485667774357 26.25644427163849 27.648678210710496 22.727391849876653 50-51 22.970732945329175 26.304701781574174 27.457787076690693 23.266778196405955 52-53 22.535730180585 26.75305317258992 27.36108233297819 23.350134313846894 54-55 22.552729175341643 26.694404116602115 27.257684414374083 23.495182293682156 56-57 23.262706578354102 26.30756201603493 27.08495593720527 23.3447754684057 58-59 22.499008812716255 26.509428424783867 27.724738210848333 23.266824551651546 60-61 22.535583831697526 26.98521413134133 27.242914727845797 23.23628730911535 62-63 22.811714178172128 26.454479192081674 26.965144606541962 23.768662023204236 64-65 22.222704128235087 26.493973348111332 27.315549877578842 23.967772646074735 66-67 22.654319928104393 26.23906209578643 27.2758105114225 23.830807464686675 68-69 22.4286393659019 26.12206339356446 27.55915358975227 23.890143650781365 70-71 22.3383420447466 25.94781734461411 27.60906763484555 24.10477297579374 72-73 22.769767920875598 25.990342399160397 27.783701986324633 23.45618769363937 74-75 22.86698981333958 26.349347831817422 27.39205983628516 23.391602518557843 76-77 22.720909631500543 26.32912071407115 27.00542848560271 23.944541168825594 78-79 22.470117177262907 26.33443198192303 27.219000929657167 23.976449911156898 80-81 22.227918430703248 26.647374144955684 27.589945941704336 23.534761482636732 82-83 22.812966672960496 25.95011243063508 28.304580965996166 22.932339930408258 84-85 23.048820149825843 26.33664804904238 27.16221058565359 23.452321215478182 86-87 22.05883660228891 26.819684543360346 27.488133208470828 23.63334564587992 88-89 22.701778704469476 25.917798754361236 27.352508780717184 24.027913760452105 90-91 22.37419330711781 25.978901801833143 27.585873412343652 24.06103147870539 92-93 22.620231623447058 26.208753539943253 27.357766586062404 23.813248250547286 94-95 21.730547300890585 26.10857127969189 27.85901151429977 24.301869905117755 96-97 21.990379931993743 25.974152964108356 27.566718071376222 24.46874903252168 98-99 21.542177013477463 26.529094040316064 27.413323099704474 24.515405846502002 100 22.13985520584085 26.0093870789619 26.860666298545922 24.990091416651328 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 178.0 1 147.5 2 171.5 3 231.0 4 172.0 5 135.5 6 278.0 7 698.5 8 1200.5 9 1396.5 10 1159.5 11 825.5 12 755.5 13 845.0 14 986.5 15 1266.0 16 1589.0 17 1988.0 18 2527.5 19 3083.5 20 3875.5 21 4756.0 22 5759.5 23 6809.0 24 7853.0 25 9278.5 26 11209.0 27 13667.0 28 17107.5 29 22898.5 30 30855.0 31 38328.5 32 48933.5 33 57826.5 34 62679.0 35 70665.0 36 78933.0 37 92089.5 38 99048.0 39 94823.5 40 90553.5 41 87052.0 42 87482.0 43 92692.0 44 100066.0 45 115088.5 46 129622.0 47 138195.5 48 144224.5 49 142347.5 50 137107.0 51 138811.5 52 139460.0 53 134555.0 54 130084.0 55 115635.5 56 102076.5 57 87716.5 58 72083.5 59 66939.5 60 56285.5 61 41585.5 62 32048.0 63 25519.5 64 18538.0 65 11129.0 66 7334.5 67 5438.5 68 4051.0 69 3031.0 70 2242.5 71 1667.5 72 1306.0 73 1082.0 74 846.0 75 623.0 76 455.0 77 286.0 78 151.5 79 70.5 80 39.5 81 19.5 82 11.0 83 7.0 84 2.5 85 1.0 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01265897279025829 2 0.00481224873624831 3 0.0020842860768464015 4 0.02651334494811967 5 0.0026973113935659316 6 0.0 7 4.597689875396474E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.008781587662007265 16-17 0.002743288292319896 18-19 0.0 20-21 7.662816458994124E-5 22-23 7.969329117353889E-4 24-25 0.0 26-27 2.1455886085183546E-4 28-29 0.0 30-31 0.0 32-33 0.0 34-35 5.363971521295886E-4 36-37 0.0 38-39 0.0 40-41 3.9846645586769444E-4 42-43 7.509560129814241E-4 44-45 0.0 46-47 0.0 48-49 5.363971521295886E-4 50-51 0.0 52-53 0.0 54-55 3.524895571137297E-4 56-57 1.532563291798825E-4 58-59 1.9923322793384722E-4 60-61 3.065126583597649E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0014559351272088836 70-71 7.816072788174006E-4 72-73 0.0 74-75 1.8390759501585896E-4 76-77 0.0026973113935659316 78-79 4.597689875396474E-4 80-81 0.0031877316469415555 82-83 0.0015938658234707778 84-85 0.0057317867113276045 86-87 0.007754770256502053 88-89 0.007540211395650217 90-91 0.005869717407589499 92-93 0.008383121206139571 94-95 0.0013180044309469893 96-97 0.004934853799592216 98-99 0.0018850528489125543 100 0.08300362788382434 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3262508.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.635533321690275 #Duplication Level Percentage of deduplicated Percentage of total 1 71.21143327289133 26.088688365543334 2 15.629732449085662 11.45207167895163 3 5.517362572213587 6.0639456108653285 4 2.604788866276968 3.817113172258307 5 1.3461766459009585 2.4658949683892906 6 0.8124097290507668 1.7857838219702844 7 0.518286988531696 1.3291404166986016 8 0.36383153320231454 1.0663329806492043 9 0.2647866640262738 0.8730540587766387 >10 1.3565104592749126 9.378592714006041 >50 0.14920161942946053 3.864117480023585 >100 0.1812043070272054 14.269452719015632 >500 0.03046514382681601 7.5057261881474515 >1k 0.01305649021148419 8.130040759247558 >5k 7.532590506625494E-4 1.9100450654570587 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 9491 0.2909111640492529 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7376 0.22608373680616262 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 7371 0.22593048047698272 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7067 0.2166124956628459 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 6999 0.2145282095859995 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 6388 0.19580028616021783 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 6194 0.1898539405880384 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 6153 0.18859723868876338 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 5254 0.16104175070222052 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4121 0.12631386651005913 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 3889 0.11920277283611258 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 3830 0.11739434815178998 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 3783 0.11595373865749907 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 3561 0.1091491576419123 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 3524 0.10801506080598117 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 3265 0.10007638295446326 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.1302531671953E-5 2 0.0 0.0 0.0 0.0 6.1302531671953E-5 3 0.0 0.0 0.0 0.0 6.1302531671953E-5 4 0.0 0.0 0.0 0.0 6.1302531671953E-5 5 0.0 0.0 0.0 0.0 6.1302531671953E-5 6 0.0 0.0 0.0 0.0 1.22605063343906E-4 7 0.0 0.0 0.0 0.0 1.22605063343906E-4 8 0.0 0.0 0.0 0.0 1.22605063343906E-4 9 0.0 0.0 0.0 0.0 1.22605063343906E-4 10-11 0.0 0.0 0.0 0.0 1.22605063343906E-4 12-13 0.0 0.0 0.0 0.0 1.5325632917988246E-4 14-15 0.0 0.0 0.0 0.0 2.1455886085183546E-4 16-17 0.0 0.0 0.0 0.0 2.45210126687812E-4 18-19 0.0 0.0 0.0 1.532563291798825E-5 2.45210126687812E-4 20-21 0.0 0.0 0.0 4.5976898753964746E-5 3.3716392419574145E-4 22-23 0.0 0.0 0.0 9.195379750792948E-5 3.678151900317179E-4 24-25 0.0 0.0 0.0 2.1455886085183546E-4 3.678151900317179E-4 26-27 0.0 0.0 0.0 3.524895571137297E-4 3.9846645586769444E-4 28-29 0.0 0.0 0.0 0.0014712607601268717 3.9846645586769444E-4 30-31 0.0 0.0 0.0 0.005992322470933405 4.1379208878568265E-4 32-33 0.0 0.0 0.0 0.016245170893067543 4.291177217036709E-4 34-35 0.0 0.0 0.0 0.03270490064698692 4.5976898753964743E-4 36-37 0.0 0.0 0.0 0.06070483198815145 4.5976898753964743E-4 38-39 0.0 0.0 0.0 0.1074480123880156 5.210715192116004E-4 40-41 0.0 0.0 0.0 0.16094979690471256 5.210715192116004E-4 42-43 0.0 0.0 0.0 0.2160454472448803 5.517227850475769E-4 44-45 0.0 0.0 0.0 0.28589968208507077 5.976996838015416E-4 46-47 0.0 0.0 0.0 0.3757998447819898 6.743278483914829E-4 48-49 0.0 0.0 0.0 0.4828034138153837 7.662816458994124E-4 50-51 0.0 0.0 0.0 0.6001517850684197 7.969329117353889E-4 52-53 0.0 0.0 0.0 0.7140672145478264 8.582354434073418E-4 54-55 0.0 0.0 0.0 0.8403964066908036 9.195379750792949E-4 56-57 0.0 0.0 0.0 1.000242758025421 9.655148738332597E-4 58-59 0.0 0.0 0.0 1.1917978438673562 9.80840506751248E-4 60-61 0.0 0.0 0.0 1.3970387199050547 0.0010421430384232008 62-63 0.0 0.0 0.0 1.6096205741104697 0.0011340968359311303 64-65 0.0 0.0 0.0 1.8310299928766458 0.0011800737346850951 66-67 0.0 0.0 0.0 2.087167295834983 0.0011953993676030834 68-69 0.0 0.0 0.0 2.379319836150593 0.0011953993676030834 70-71 0.0 0.0 0.0 2.706966542304264 0.0012567018992750362 72-73 0.0 0.0 0.0 3.0500308351734313 0.0013639813297009538 74-75 0.0 0.0 0.0 3.3884514612684473 0.0014406094942908951 76-77 0.0 0.0 0.0 3.771975425041103 0.0015019120259628482 78-79 0.0 0.0 0.0 4.200480121428055 0.0015172376588808364 80-81 0.0 0.0 0.0 4.667498133337911 0.001547888924716813 82-83 0.0 0.0 0.0 5.15612528766213 0.0015632145576348012 84-85 0.0 0.0 0.0 5.632231399892353 0.0015632145576348012 86-87 0.0 0.0 0.0 6.135632464349513 0.0016551683551427306 88 0.0 0.0 0.0 6.548305781932182 0.0017471221526506602 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6315 0.0 47.3531 1 GTATCAA 10715 0.0 37.91283 1 GGGCACC 2880 0.0 34.626972 94 GTACTAG 2680 0.0 33.509136 1 GTGGTAT 1880 0.0 33.262775 1 CAACGCA 12685 0.0 30.826042 5 ATCAACG 12965 0.0 30.13329 3 TATCAAC 13960 0.0 29.031303 2 AACGCAG 14340 0.0 27.824677 6 TAGTACT 3910 0.0 27.65344 4 TCAACGC 14635 0.0 26.918406 4 CTGTGCG 3850 0.0 26.860458 9 CTAACGC 1235 0.0 25.885704 3 CTAGTAC 4305 0.0 25.444904 3 ACGCAGA 15390 0.0 25.346098 7 CGCAGAG 15580 0.0 24.946117 8 GCATCGT 3380 0.0 24.888645 8 CGCCTAC 1260 0.0 24.617558 7 GTACATG 12045 0.0 24.475151 1 TCTAACG 1330 0.0 24.391703 2 >>END_MODULE