##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146476_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4375708 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.424512787416344 31.0 30.0 34.0 27.0 34.0 2 30.52742916117803 31.0 30.0 34.0 26.0 34.0 3 30.71100676736199 31.0 31.0 34.0 27.0 34.0 4 34.18534029235955 37.0 35.0 37.0 30.0 37.0 5 34.39650132047203 37.0 35.0 37.0 30.0 37.0 6 34.61283408307867 37.0 35.0 37.0 31.0 37.0 7 34.514544617693865 37.0 35.0 37.0 30.0 37.0 8 34.447019316645445 37.0 35.0 37.0 30.0 37.0 9 35.83083628980727 39.0 35.0 39.0 30.0 39.0 10-11 35.7380573612316 39.0 35.0 39.0 30.0 39.0 12-13 35.71814595489461 39.0 35.0 39.0 30.0 39.0 14-15 36.72625492834531 40.0 36.0 41.0 29.5 41.0 16-17 36.56792855007693 40.0 36.0 41.0 28.0 41.0 18-19 36.5186895469259 40.0 36.0 41.0 27.5 41.0 20-21 36.85453496896959 40.0 36.0 41.0 30.0 41.0 22-23 36.57330859371786 40.0 36.0 41.0 28.5 41.0 24-25 36.68385984622374 40.0 36.0 41.0 29.5 41.0 26-27 36.75464953328695 40.0 36.0 41.0 30.0 41.0 28-29 36.59479780186429 40.0 35.0 41.0 29.5 41.0 30-31 36.54341114626479 39.0 35.0 41.0 29.5 41.0 32-33 36.64646955418415 39.0 35.0 41.0 30.0 41.0 34-35 36.698613801469385 40.0 35.0 41.0 30.0 41.0 36-37 36.63172885393632 39.5 35.0 41.0 30.0 41.0 38-39 36.45276650087254 39.0 35.0 41.0 29.0 41.0 40-41 36.41477356350104 39.0 35.0 41.0 29.0 41.0 42-43 36.25180953573685 39.0 35.0 41.0 28.0 41.0 44-45 36.049551066935905 39.0 35.0 41.0 28.0 41.0 46-47 35.91007558091171 38.5 34.5 40.5 28.0 41.0 48-49 35.77362909042377 38.0 34.0 40.0 28.0 41.0 50-51 35.54158264216899 38.0 34.0 40.0 27.0 41.0 52-53 35.33470217848175 38.0 34.0 40.0 27.0 41.0 54-55 35.13116483092565 37.0 33.0 40.0 27.0 41.0 56-57 34.964343484528676 37.0 33.0 40.0 27.0 41.0 58-59 34.687299061089085 36.5 33.0 40.0 26.0 41.0 60-61 34.42552073858676 36.0 33.0 39.5 26.0 41.0 62-63 34.14300988548597 35.5 33.0 39.0 26.0 41.0 64-65 33.85527313522749 35.0 32.5 39.0 26.0 41.0 66-67 33.566971447820556 35.0 32.0 38.0 26.0 40.0 68-69 33.27998040545667 35.0 32.0 37.5 25.0 40.0 70-71 33.2244663035102 35.0 32.0 37.0 25.0 40.0 72-73 33.02804563284387 35.0 32.0 37.0 25.0 40.0 74-75 32.77856989086109 35.0 32.0 36.0 25.0 39.0 76-77 32.55885527553484 35.0 32.0 36.0 25.0 39.0 78-79 32.357840034115625 35.0 31.0 35.5 24.0 39.0 80-81 32.15126900606713 35.0 31.0 35.0 24.0 37.5 82-83 31.92514045727 35.0 31.0 35.0 24.0 37.0 84-85 31.760326671706615 35.0 31.0 35.0 24.0 36.5 86-87 31.60511716046866 34.5 31.0 35.0 24.0 36.0 88-89 31.624574240328652 35.0 31.0 35.0 24.0 36.0 90-91 31.55653096596025 35.0 31.0 35.0 24.0 36.0 92-93 31.416865796346556 35.0 31.0 35.0 23.0 36.0 94-95 31.407968150525583 35.0 31.0 35.0 23.5 35.0 96-97 31.321158998726606 35.0 31.0 35.0 23.0 35.0 98-99 31.24354230218287 35.0 31.0 35.0 23.0 35.0 100 30.754366150574945 34.0 30.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8574999846030771 1101 2 0.9612099289564497 1101 3 0.9906923352535806 1101 4 1.0551582572099178 1101 5 0.9764817763697593 1101 6 0.7611698813004963 1101 7 0.7364382911095362 1101 8 0.8220235945294334 1101 9 1.0236134289492185 1101 10-11 1.042109872382774 1101 12-13 0.914623166690042 1101 14-15 1.1632515982824643 1101 16-17 1.2416081244835766 1101 18-19 1.3898041666709204 1101 20-21 1.054781451899835 1101 22-23 1.3607940826906955 1101 24-25 1.198196927745407 1101 26-27 1.1029437882043212 1101 28-29 1.2947292552136105 1101 30-31 1.2985537832795728 1101 32-33 1.125394275489306 1101 34-35 1.074571933754271 1101 36-37 1.0420932766577735 1101 38-39 1.0985929625994615 1101 40-41 1.0115004229449127 1101 42-43 1.0559997679740363 1101 44-45 1.0379386576752552 1101 46-47 0.9717088076463938 1101 48-49 1.0543853761615622 1101 50-51 1.0199583064321232 1101 52-53 1.0665630994316828 1101 54-55 1.0135723584727288 1101 56-57 0.9264993535139894 1101 58-59 0.8863075235609017 1101 60-61 0.9204166438702259 1101 62-63 0.8307983711627003 1101 64-65 0.8130624149428556 1101 66-67 0.8999861039501553 1101 68-69 0.8779958348553407 1101 70-71 0.7596428425555501 1101 72-73 0.7313012272283359 1101 74-75 0.7006761639077013 1101 76-77 0.7472876710335683 1101 78-79 0.796255528001204 1101 80-81 0.7945131216722032 1101 82-83 0.7976556734175766 1101 84-85 0.7531439763477117 1101 86-87 0.773718673376095 1101 88-89 0.7357021043175678 1101 90-91 0.7589161666030755 1101 92-93 0.7960554551835095 1101 94-95 0.7367167057516113 1101 96-97 0.7847589883984192 1101 98-99 0.8038507726542825 1101 100 0.8443636336797695 1102 1 0.8250068176650416 1102 2 0.9731275273850066 1102 3 0.9507663436480307 1102 4 1.0939872034225502 1102 5 0.900988217334266 1102 6 0.7581180773339469 1102 7 0.8040784436710879 1102 8 0.8404838418308955 1102 9 1.0212476363967795 1102 10-11 1.0335391057940555 1102 12-13 0.9861429198723286 1102 14-15 1.2247348342067426 1102 16-17 1.4014219704885775 1102 18-19 1.3963151485822394 1102 20-21 1.1786187651920628 1102 22-23 1.3921731772765682 1102 24-25 1.2781221109682193 1102 26-27 1.145450239353174 1102 28-29 1.3517343212909338 1102 30-31 1.3981324535599953 1102 32-33 1.1985860124173797 1102 34-35 1.1018332889856524 1102 36-37 1.1055281681117108 1102 38-39 1.1564302014186367 1102 40-41 1.0571502576401883 1102 42-43 1.114364285573231 1102 44-45 1.1413854711832698 1102 46-47 1.1396250197303814 1102 48-49 1.0811818317533408 1102 50-51 1.082715859082036 1102 52-53 1.02652985389593 1102 54-55 1.045766917102128 1102 56-57 0.9820704154488382 1102 58-59 0.9598622370687764 1102 60-61 0.9826539990455885 1102 62-63 0.9571380109422591 1102 64-65 0.8964731917668374 1102 66-67 0.9774197815569323 1102 68-69 0.9833663764972371 1102 70-71 0.9045390831302456 1102 72-73 0.8035572770501673 1102 74-75 0.8451331248469103 1102 76-77 0.8563624515290158 1102 78-79 0.8466797538951667 1102 80-81 0.8682673847772335 1102 82-83 0.8167839503911445 1102 84-85 0.791777908181313 1102 86-87 0.8363242564364626 1102 88-89 0.822024650303085 1102 90-91 0.7795662081679531 1102 92-93 0.9248460223117441 1102 94-95 0.8687878268829934 1102 96-97 0.8752354759181351 1102 98-99 0.8560960079700415 1102 100 0.8087142731932637 1103 1 0.6626828939859166 1103 2 0.8437011306369087 1103 3 0.7865982523063728 1103 4 0.7480812606195073 1103 5 0.7081465813595287 1103 6 0.49977037939659397 1103 7 0.6309444188785704 1103 8 0.6732985943744154 1103 9 0.779435302038479 1103 10-11 0.8604289011322166 1103 12-13 0.7881104898636195 1103 14-15 0.9078520774792196 1103 16-17 1.1358071722357934 1103 18-19 1.2283836352428779 1103 20-21 0.8889983066778981 1103 22-23 1.0709328324753002 1103 24-25 0.9557538795796034 1103 26-27 0.8370935709041945 1103 28-29 1.037147283485325 1103 30-31 1.0769724875346185 1103 32-33 0.9470811512975743 1103 34-35 0.8907661820341275 1103 36-37 0.937946783496237 1103 38-39 1.0453366022209138 1103 40-41 0.9404175768016572 1103 42-43 0.9272147882573165 1103 44-45 1.037874866669597 1103 46-47 0.9434506505717479 1103 48-49 0.929159559319217 1103 50-51 1.0052571909750085 1103 52-53 1.0056718731866567 1103 54-55 0.929322366921383 1103 56-57 0.8719291455289948 1103 58-59 0.8805151606759267 1103 60-61 0.834622992352017 1103 62-63 0.7711877699487246 1103 64-65 0.8031672241600418 1103 66-67 0.8714174576550135 1103 68-69 0.867385740926288 1103 70-71 0.8302833273616983 1103 72-73 0.756416807813082 1103 74-75 0.7589592692602665 1103 76-77 0.7452175064644493 1103 78-79 0.721523472533498 1103 80-81 0.838838100603212 1103 82-83 0.7548239828971255 1103 84-85 0.7403351176532276 1103 86-87 0.8133606305692638 1103 88-89 0.7824930243314441 1103 90-91 0.7902120614345058 1103 92-93 0.8714186137407047 1103 94-95 0.8643204078356277 1103 96-97 0.8005330077083777 1103 98-99 0.8964535420122068 1103 100 0.9645503729254088 1104 1 0.6156597142542886 1104 2 0.7742164591647693 1104 3 0.6628220048237026 1104 4 0.8233485820659254 1104 5 0.7205471207706964 1104 6 0.4535498868270409 1104 7 0.5044887059464571 1104 8 0.5047812653964812 1104 9 0.6077290607380448 1104 10-11 0.6463714765826225 1104 12-13 0.5710032584745264 1104 14-15 0.73712466296287 1104 16-17 0.8454011574333293 1104 18-19 0.8564903833347799 1104 20-21 0.7019975388344974 1104 22-23 0.9397848368444457 1104 24-25 0.8429070164520525 1104 26-27 0.7762391680438014 1104 28-29 0.9667437677290422 1104 30-31 0.969489744803127 1104 32-33 0.8689197416252554 1104 34-35 0.7098589959217065 1104 36-37 0.7645045542931328 1104 38-39 0.8369057383536216 1104 40-41 0.7701273605741719 1104 42-43 0.6994207646575035 1104 44-45 0.7768533687394381 1104 46-47 0.7243648728336041 1104 48-49 0.766056830391797 1104 50-51 0.723260385683389 1104 52-53 0.7006801699795275 1104 54-55 0.6928454383812266 1104 56-57 0.6536766527675084 1104 58-59 0.6560096607861468 1104 60-61 0.7032527918871381 1104 62-63 0.5806706122915983 1104 64-65 0.5875681482679624 1104 66-67 0.6576191035882886 1104 68-69 0.5953286424388651 1104 70-71 0.5474213587535672 1104 72-73 0.5612388414206748 1104 74-75 0.5966815018951834 1104 76-77 0.6094905546221057 1104 78-79 0.5949040882518446 1104 80-81 0.6014299269025187 1104 82-83 0.5943177885582678 1104 84-85 0.5687879647697649 1104 86-87 0.6077977052537449 1104 88-89 0.5526805896087481 1104 90-91 0.5880224777797771 1104 92-93 0.633170550248753 1104 94-95 0.6296768545154663 1104 96-97 0.6510958423761863 1104 98-99 0.6850942516227043 1104 100 0.7057817570364229 1105 1 0.44986175517974303 1105 2 0.605476809649204 1105 3 0.55069556600602 1105 4 0.6687131241208917 1105 5 0.5759769014787537 1105 6 0.3203003989691098 1105 7 0.39876087507362 1105 8 0.39358523403809187 1105 9 0.40859021838943477 1105 10-11 0.5522294543688133 1105 12-13 0.4981605279860446 1105 14-15 0.6388498111520207 1105 16-17 0.8246179884140332 1105 18-19 0.8204786587677617 1105 20-21 0.6245013778267676 1105 22-23 0.8581445345973648 1105 24-25 0.7173990706370077 1105 26-27 0.5516370227195111 1105 28-29 0.787620428633069 1105 30-31 0.8077069211704071 1105 32-33 0.6787402507407165 1105 34-35 0.5356391708388415 1105 36-37 0.48569466379115767 1105 38-39 0.589885742421842 1105 40-41 0.4918569458462443 1105 42-43 0.5042181084583177 1105 44-45 0.5501837139415642 1105 46-47 0.522540228632856 1105 48-49 0.49915182974361016 1105 50-51 0.5852310489224593 1105 52-53 0.5849053450379742 1105 54-55 0.5718915730305056 1105 56-57 0.48716937742435107 1105 58-59 0.5466449876355597 1105 60-61 0.507374190422766 1105 62-63 0.41329373371308975 1105 64-65 0.4527293249069828 1105 66-67 0.553352697096031 1105 68-69 0.5314400885868835 1105 70-71 0.43496124307361583 1105 72-73 0.39147281793199795 1105 74-75 0.4713166267936231 1105 76-77 0.4927655185542221 1105 78-79 0.5007967307483483 1105 80-81 0.5371496120660098 1105 82-83 0.5279851570111695 1105 84-85 0.4941421054213144 1105 86-87 0.50971462061068 1105 88-89 0.5123734927838797 1105 90-91 0.5255773690997678 1105 92-93 0.5661634993471623 1105 94-95 0.4794911180121133 1105 96-97 0.5382526899402329 1105 98-99 0.52908023288213 1105 100 0.6692933844095599 1106 1 0.49042350281434466 1106 2 0.5478439628126885 1106 3 0.518080219687711 1106 4 0.6842763276891688 1106 5 0.5700173230615562 1106 6 0.30505317969386425 1106 7 0.33168560892655563 1106 8 0.39582070565485594 1106 9 0.47538039947718147 1106 10-11 0.4686923988916405 1106 12-13 0.43234956815161496 1106 14-15 0.5626158753335773 1106 16-17 0.7160422285523751 1106 18-19 0.6590733846355405 1106 20-21 0.5341304345889029 1106 22-23 0.8623557223261287 1106 24-25 0.668103501508952 1106 26-27 0.5411444963169529 1106 28-29 0.7186466076016984 1106 30-31 0.746581527216037 1106 32-33 0.6226678694920338 1106 34-35 0.559816865795959 1106 36-37 0.477851513252304 1106 38-39 0.5922679365418944 1106 40-41 0.5735106246291011 1106 42-43 0.5126625672051475 1106 44-45 0.6209246324005591 1106 46-47 0.5125885691051479 1106 48-49 0.5112164496606795 1106 50-51 0.5209684056096293 1106 52-53 0.5261763679711109 1106 54-55 0.5721635536010297 1106 56-57 0.4323113925173345 1106 58-59 0.44183984628100603 1106 60-61 0.4548493690878672 1106 62-63 0.4294932704113279 1106 64-65 0.4426231692107194 1106 66-67 0.48931252609450837 1106 68-69 0.4780954387077756 1106 70-71 0.4792194445401847 1106 72-73 0.4878763968406261 1106 74-75 0.49280074673720264 1106 76-77 0.46190857314693545 1106 78-79 0.4421221041958816 1106 80-81 0.4892481781461342 1106 82-83 0.5800289665223239 1106 84-85 0.45776911479978466 1106 86-87 0.47610272514832275 1106 88-89 0.5558894829310361 1106 90-91 0.5667859595123339 1106 92-93 0.5804716251160364 1106 94-95 0.5317259903183675 1106 96-97 0.5903686447107006 1106 98-99 0.5480543817971544 1106 100 0.5818258400048855 1107 1 0.47870821598027646 1107 2 0.619710760472632 1107 3 0.560703790502231 1107 4 0.6259277296697618 1107 5 0.5778135149375174 1107 6 0.36179384048691077 1107 7 0.32111456491941226 1107 8 0.46068337560240025 1107 9 0.5051650321111225 1107 10-11 0.5408672536782646 1107 12-13 0.45763087619452136 1107 14-15 0.637638092146716 1107 16-17 0.7187436377367646 1107 18-19 0.8199466954879782 1107 20-21 0.523211158618814 1107 22-23 0.8737354967263045 1107 24-25 0.6177973995004322 1107 26-27 0.4982998459310153 1107 28-29 0.6467071606249348 1107 30-31 0.6902510229733707 1107 32-33 0.6249453944724479 1107 34-35 0.4901733474352383 1107 36-37 0.4430095808559784 1107 38-39 0.5445838914450931 1107 40-41 0.40824722087841536 1107 42-43 0.4998200576896892 1107 44-45 0.4710870573545236 1107 46-47 0.4167504461970495 1107 48-49 0.4379880510834866 1107 50-51 0.5065062152891358 1107 52-53 0.5523066134174783 1107 54-55 0.5067402805993808 1107 56-57 0.4782077968223035 1107 58-59 0.46990969120978576 1107 60-61 0.536892841006221 1107 62-63 0.5272456288168499 1107 64-65 0.5753230985969537 1107 66-67 0.529433706527719 1107 68-69 0.5356556833199804 1107 70-71 0.4955057826313123 1107 72-73 0.46475969310250065 1107 74-75 0.4121435426136628 1107 76-77 0.42833507594374254 1107 78-79 0.3961924103309187 1107 80-81 0.4902022286222447 1107 82-83 0.48697544452441477 1107 84-85 0.47492433829102865 1107 86-87 0.5875653094739164 1107 88-89 0.49571794903540933 1107 90-91 0.5046035281333907 1107 92-93 0.5348550611747562 1107 94-95 0.47792492422449584 1107 96-97 0.4941691413214393 1107 98-99 0.5043469591068472 1107 100 0.4272196427371746 1108 1 0.4170501187760074 1108 2 0.5599662529001499 1108 3 0.5052126857759056 1108 4 0.651066518939281 1108 5 0.47250772289210374 1108 6 0.2732071553591737 1108 7 0.28592791662735095 1108 8 0.32405545002529834 1108 9 0.5167634237591088 1108 10-11 0.48199180358140126 1108 12-13 0.3973990682169486 1108 14-15 0.6146521477655753 1108 16-17 0.7250630852886175 1108 18-19 0.7744798808092952 1108 20-21 0.4676577598422895 1108 22-23 0.66722646542474 1108 24-25 0.5497846413170109 1108 26-27 0.5122736520100162 1108 28-29 0.6457982685656063 1108 30-31 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-1.1247162628224956 2216 5 -1.183278608003917 2216 6 -1.0937233498997188 2216 7 -1.244838991545791 2216 8 -1.1168609436061914 2216 9 -1.3968191320824275 2216 10-11 -1.3355910937688336 2216 12-13 -1.449796557525751 2216 14-15 -1.9167604277312194 2216 16-17 -1.7055928403500857 2216 18-19 -3.587644592838565 2216 20-21 -1.851828919925282 2216 22-23 -1.3180543790135815 2216 24-25 -1.6004742870809778 2216 26-27 -1.4528425044942566 2216 28-29 -1.3750928852192388 2216 30-31 -1.5296657443484705 2216 32-33 -1.6128258680473877 2216 34-35 -1.8714864712467687 2216 36-37 -1.8173306054946963 2216 38-39 -1.8440582261213265 2216 40-41 -1.858744487161708 2216 42-43 -1.868120337310316 2216 44-45 -1.8027968338279905 2216 46-47 -1.9476314461445838 2216 48-49 -1.9432071574723153 2216 50-51 -1.733089158634975 2216 52-53 -1.743287018264006 2216 54-55 -1.6883815005329623 2216 56-57 -1.761182105376193 2216 58-59 -1.827385833895562 2216 60-61 -1.7401418864755698 2216 62-63 -1.7200629763725672 2216 64-65 -1.7735819069096337 2216 66-67 -1.7014350051445675 2216 68-69 -1.8167808473544511 2216 70-71 -1.949936895068177 2216 72-73 -1.8813873077700798 2216 74-75 -1.9233098580043553 2216 76-77 -1.817619270924876 2216 78-79 -1.9042841959031165 2216 80-81 -1.841351959042111 2216 82-83 -2.019599221552088 2216 84-85 -1.853173707077623 2216 86-87 -1.8480265658422397 2216 88-89 -1.7352310318874444 2216 90-91 -1.8269585108975157 2216 92-93 -1.789049135299713 2216 94-95 -1.7478768958529614 2216 96-97 -1.7510750031147602 2216 98-99 -1.7387705280984953 2216 100 -1.6695297140018255 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 18082.0 1 2887.0 2 517.0 3 472.0 4 437.0 5 267.0 6 230.0 7 307.0 8 539.0 9 994.0 10 1624.0 11 2915.0 12 4839.0 13 7205.0 14 9367.0 15 11984.0 16 14444.0 17 17374.0 18 20354.0 19 23195.0 20 26720.0 21 30355.0 22 34677.0 23 39836.0 24 45401.0 25 51978.0 26 58521.0 27 67060.0 28 79231.0 29 94627.0 30 113088.0 31 134580.0 32 166325.0 33 212949.0 34 297146.0 35 400707.0 36 590632.0 37 891967.0 38 671782.0 39 229982.0 40 111.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.269370487713566 21.49827645026773 12.573349540132833 23.659003521885875 2 15.36725905565007 19.68400003397454 41.112707557476085 23.836033352899303 3 18.436900300198655 27.19563753234088 28.844651960843976 25.522810206616498 4 12.108925755962646 16.87121318398028 40.29389216667926 30.725968893377818 5 13.07569158733711 38.82189438409851 34.89238149340403 13.210032535160352 6 29.498163930360256 38.68965026260733 18.32050725586245 13.491678551169963 7 26.866830038149363 31.863709687236792 23.927751185951553 17.341709088662295 8 26.348174670771858 34.93323557326997 19.913352793466245 18.805236962491925 9 26.315236910921698 14.97901525021233 21.855819641192824 36.84992819767315 10-11 25.896374660569233 25.80466502547432 27.272134068159225 21.026826245797217 12-13 26.02084915791404 23.73379229786927 27.380878530173884 22.864480014042808 14-15 23.48557490445437 24.77392598256428 24.797165492073926 26.943333620907424 16-17 22.185276362726086 27.31344437540072 26.34783171115047 24.153447550722724 18-19 21.61218184959645 28.139469214749568 27.96236924287235 22.285979692781638 20-21 23.526235048332648 27.15710563336757 26.684416859412913 22.632242458886864 22-23 24.10769375814827 26.81828501320087 26.456990743255414 22.617030485395443 24-25 23.476709940915722 26.91222067994536 27.17338024264841 22.43768913649051 26-27 23.25887971803214 27.163640222685515 26.113266767798255 23.46421329148409 28-29 22.235650532326186 27.76882897427569 26.833783657749155 23.16173683564897 30-31 23.186809198155846 27.223608621450524 26.721656291070783 22.867925889322844 32-33 23.714620991665555 27.572211071288354 25.553797595579407 23.159370341466687 34-35 23.635229866815184 27.317195560866114 26.692462012636664 22.355112559682038 36-37 23.299170492976025 26.859192236899844 26.38285736806371 23.45877990206042 38-39 23.472775461420735 26.895576433980494 27.009466062947084 22.622182041651687 40-41 22.963543223729154 25.962765846218964 27.78582295968276 23.287867970369117 42-43 23.02506346635686 26.259607271470564 26.938446825211535 23.776882436961042 44-45 23.106577712012545 27.350028628023104 26.740193709970033 22.80319994999432 46-47 23.117108742724547 27.027478250478428 26.211283036493466 23.644129970303567 48-49 23.937739092917525 26.45469406387344 26.528325177050665 23.07924166615837 50-51 23.470595479960643 26.399041746877245 26.47169656114332 23.65866621201879 52-53 22.796038887853076 27.09166266605965 26.326797151361024 23.78550129472624 54-55 22.8203674709066 27.003335004588674 26.235817498916596 23.94048002558813 56-57 24.02041838950849 26.30478200465153 26.06168296402084 23.613116641819147 58-59 22.748732189390566 26.72299950841931 27.091472493825947 23.436795808364177 60-61 22.76536078608774 27.528716790198395 26.24364373144928 23.462278692264587 62-63 23.397747989226865 26.617282339644998 25.77959277629874 24.205376894829396 64-65 22.342704929643237 26.891186572965637 26.324263412886843 24.44184508450428 66-67 23.22866698395926 26.405797471056243 26.262740096122883 24.102795448861613 68-69 22.55149914242304 26.501615705556546 26.529560063651548 24.417325088368862 70-71 22.49952897818135 26.13292235722295 26.80168143300921 24.565867231586495 72-73 23.167606891584597 26.325271368522714 27.190137363637902 23.316984376254794 74-75 23.36737193190779 26.698422137136575 26.658166782163384 23.276039148792247 76-77 23.007723229593058 26.58222250046091 26.300126758144653 24.109927511801374 78-79 22.797985688390703 26.550948122570805 26.421977024437748 24.229089164600744 80-81 22.348073381342566 26.937405571423845 27.175556278512374 23.53896476872121 82-83 23.302287694133845 25.755389832284166 28.265423845518413 22.676898628063576 84-85 23.531122214459756 26.486081963576314 26.671140327386027 23.3116554945779 86-87 22.067360546130462 27.194662690227222 27.236862423338685 23.501114340303626 88-89 23.22440041331801 25.714175784015342 27.047476457384466 24.013947345282183 90-91 22.790028615861775 25.783632013580892 27.344512955583316 24.081826414974014 92-93 23.055582801517193 26.109853175652574 27.237362312099624 23.59720171073061 94-95 21.73752397241954 26.038723971171745 28.010484723796246 24.21326733261246 96-97 22.22905764409464 25.944481047647205 27.442362663762747 24.38409864449541 98-99 21.463084179574434 26.794186516263853 27.298609866822947 24.444119437338767 100 22.323560302806687 25.94527743648439 26.474098642748455 25.25706361796047 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 15200.0 1 8945.5 2 2497.5 3 1834.0 4 1023.5 5 581.5 6 442.5 7 417.5 8 417.5 9 423.0 10 414.5 11 378.5 12 370.0 13 389.0 14 387.5 15 459.5 16 536.0 17 591.0 18 723.0 19 835.0 20 992.0 21 1221.5 22 1671.5 23 2367.5 24 3087.5 25 4032.0 26 5676.5 27 7601.0 28 11078.5 29 19559.0 30 31679.0 31 42917.0 32 58806.5 33 74125.5 34 84982.5 35 98282.0 36 111501.5 37 134729.5 38 144334.5 39 132593.5 40 120124.5 41 108307.0 42 106634.0 43 114358.5 44 126639.5 45 154441.5 46 182175.0 47 200496.5 48 212431.5 49 207593.5 50 196831.5 51 201067.5 52 203088.0 53 194599.5 54 186038.0 55 162285.5 56 142570.0 57 118164.0 58 93370.0 59 88969.0 60 73568.5 61 51467.5 62 38896.0 63 31925.5 64 23094.5 65 11571.5 66 6539.5 67 4603.5 68 3309.0 69 2447.0 70 1910.0 71 1455.0 72 1041.0 73 829.5 74 659.5 75 492.5 76 366.0 77 244.0 78 151.0 79 95.0 80 66.0 81 53.5 82 37.5 83 28.5 84 29.5 85 24.0 86 20.0 87 16.5 88 15.0 89 9.5 90 3.5 91 7.0 92 10.0 93 8.5 94 5.0 95 2.5 96 2.5 97 1.5 98 0.5 99 2.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9911538886964121 2 0.44575643530144154 3 0.478208326515389 4 0.4639477771368656 5 0.4315415928119518 6 0.4867326613201795 7 0.5034842361510411 8 0.5071636407182564 9 0.5207157333167569 10-11 0.5344163733046172 12-13 0.5325309641319759 14-15 0.5303484601806153 16-17 0.534622054305269 18-19 0.5339478776920215 20-21 0.5317310935738857 22-23 0.49453711262268873 24-25 0.5301199257354468 26-27 0.5097232265041451 28-29 0.5153451738552939 30-31 0.5188874577554078 32-33 0.5212642159851617 34-35 0.5341421319704148 36-37 0.5518535514709848 38-39 0.5413409669932271 40-41 0.5461287636195102 42-43 0.543820565723307 44-45 0.5531104909194123 46-47 0.552493447917457 48-49 0.523995202604927 50-51 0.5398440663773725 52-53 0.5311940376277393 54-55 0.5413981006045193 56-57 0.529308628455098 58-59 0.5352619507517412 60-61 0.5282573700073222 62-63 0.5220298063764767 64-65 0.5337307699691113 66-67 0.5315368392954923 68-69 0.5531333443639292 70-71 0.5363246359217754 72-73 0.5404039757680357 74-75 0.5368731185901802 76-77 0.5240409094939608 78-79 0.556378533485324 80-81 0.5304741541254581 82-83 0.5287715725089517 84-85 0.5385985536512033 86-87 0.8414181202219161 88-89 1.1035814089971268 90-91 1.0938458416329426 92-93 1.0935258934097065 94-95 1.0987593322040685 96-97 1.0915376437367392 98-99 1.0877668253914567 100 1.0832989769884096 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4375708.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.87263392582328 #Duplication Level Percentage of deduplicated Percentage of total 1 73.57078516831234 18.298992071268216 2 12.396067691386946 6.166457076151856 3 4.816075331212215 3.5936543601728856 4 2.5668949816940088 2.5538175682283173 5 1.5313633377309144 1.9044519853403954 6 0.9813872841141618 1.464581199433768 7 0.6788607043244971 1.1819537649702838 8 0.514751743502671 1.0242585343056974 9 0.35945375081617387 0.8046505401583314 >10 1.9632610889706321 9.264183623892345 >50 0.23579488106724986 4.180773424693656 >100 0.2830906775910642 15.702588369838475 >500 0.05704075855258236 9.94429784386681 >1k 0.04268856769095706 18.786269668768036 >5k 0.0018400244694378044 2.9318549474296947 >10k+ 6.440085643032315E-4 2.1972150214813255 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 17212 0.39335348702427125 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 14781 0.33779676340377374 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 14774 0.3376367892921557 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 13376 0.30568767385757917 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 12606 0.28809052157959353 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 11894 0.271818869083586 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 11376 0.2599807848238502 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 9726 0.22227260137102386 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 7597 0.1736176179946194 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 7594 0.1735490576610688 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 7487 0.1711037390977643 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 7137 0.16310503351686173 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 7049 0.16109393039937767 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 6861 0.15679748283020714 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 6549 0.14966720814094542 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 6090 0.13917747710770464 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 6060 0.1384918737721987 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 5995 0.1370063998786025 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 5817 0.13293848675460063 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 5787 0.1322528834190947 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 5781 0.1321157627519935 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 5705 0.13037890096871182 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 5471 0.12503119495176554 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 5462 0.12482551395111374 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 5359 0.1224716091658767 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 5340 0.12203739372005627 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 5256 0.12011770438063965 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 4991 0.11406154158367057 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 4933 0.11273604180169244 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 4915 0.11232467980038888 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 4871 0.11131912824164684 No Hit GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT 4812 0.10997077501515183 No Hit ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA 4776 0.10914805101254473 No Hit GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG 4562 0.1042574138859357 No Hit GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC 4479 0.10236057799103597 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 4475 0.1022691642129685 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 4464 0.10201777632328299 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.5706889033728944E-5 2 0.0 0.0 0.0 0.0 4.5706889033728944E-5 3 0.0 0.0 0.0 0.0 4.5706889033728944E-5 4 0.0 0.0 0.0 0.0 4.5706889033728944E-5 5 0.0 0.0 0.0 0.0 4.5706889033728944E-5 6 0.0 0.0 0.0 0.0 1.1426722258432235E-4 7 0.0 0.0 0.0 0.0 1.1426722258432235E-4 8 0.0 0.0 0.0 0.0 1.1426722258432235E-4 9 0.0 0.0 0.0 0.0 1.1426722258432235E-4 10-11 0.0 0.0 0.0 0.0 1.8282755613491578E-4 12-13 0.0 0.0 0.0 0.0 3.199482232361026E-4 14-15 0.0 0.0 1.1426722258432236E-5 0.0 3.4280166775296707E-4 16-17 0.0 0.0 2.2853444516864472E-5 0.0 3.9993527904512823E-4 18-19 0.0 0.0 2.2853444516864472E-5 0.0 4.3421544582042496E-4 20-21 0.0 0.0 2.2853444516864472E-5 0.0 4.799223348541539E-4 22-23 0.0 0.0 2.2853444516864472E-5 4.5706889033728944E-5 5.256292238878828E-4 24-25 0.0 0.0 2.2853444516864472E-5 1.256939448427546E-4 5.256292238878828E-4 26-27 0.0 0.0 2.2853444516864472E-5 3.6565511226983156E-4 5.941895574384762E-4 28-29 0.0 0.0 2.2853444516864472E-5 0.0013483532264950038 6.170430019553407E-4 30-31 0.0 0.0 2.2853444516864472E-5 0.005210585349845099 6.284697242137729E-4 32-33 0.0 0.0 2.2853444516864472E-5 0.015426075048883518 6.513231687306374E-4 34-35 0.0 0.0 2.2853444516864472E-5 0.031172098321003136 7.084567800227986E-4 36-37 0.0 0.0 2.2853444516864472E-5 0.06048164091388182 7.084567800227986E-4 38-39 0.0 0.0 2.2853444516864472E-5 0.10733120217345399 7.427369467980954E-4 40-41 0.0 0.0 2.2853444516864472E-5 0.16149386567842278 7.541636690565275E-4 42-43 0.0 0.0 2.2853444516864472E-5 0.21831895546960628 7.541636690565275E-4 44-45 0.0 0.0 2.2853444516864472E-5 0.2897816764738415 7.541636690565275E-4 46-47 0.0 0.0 2.2853444516864472E-5 0.38183535098777155 7.655903913149597E-4 48-49 0.0 0.0 2.2853444516864472E-5 0.49261742328327207 8.455774471239854E-4 50-51 0.0 0.0 2.2853444516864472E-5 0.6095013652647754 9.141377806745788E-4 52-53 0.0 0.0 2.2853444516864472E-5 0.7250141005752669 9.369912251914433E-4 54-55 0.0 0.0 2.2853444516864472E-5 0.8544902904855626 9.598446697083078E-4 56-57 0.0 0.0 2.2853444516864472E-5 1.0160984233865697 9.598446697083078E-4 58-59 0.0 0.0 2.2853444516864472E-5 1.2148091234607064 9.598446697083078E-4 60-61 0.0 0.0 2.2853444516864472E-5 1.423678179622589 9.598446697083078E-4 62-63 0.0 0.0 2.2853444516864472E-5 1.644202492488073 0.0010284050032589012 64-65 0.0 0.0 2.2853444516864472E-5 1.878861660787237 0.0010512584477757657 66-67 0.0 0.0 2.2853444516864472E-5 2.147023978748125 0.0010741118922926302 68-69 0.0 0.0 2.2853444516864472E-5 2.4503806012649836 0.0010969653368094947 70-71 0.0 0.0 2.2853444516864472E-5 2.791182592622725 0.0011198187813263592 72-73 0.0 0.0 2.2853444516864472E-5 3.147479219362901 0.0011769523926185204 74-75 0.0 0.0 2.2853444516864472E-5 3.495994248245084 0.0012226592816522491 76-77 0.0 0.0 2.2853444516864472E-5 3.9054594136537446 0.0012340860039106814 78-79 0.0 0.0 2.2853444516864472E-5 4.3448054577682065 0.0012455127261691136 80-81 0.0 0.0 2.2853444516864472E-5 4.8299840848612385 0.0012569394484275459 82-83 0.0 0.0 2.2853444516864472E-5 5.329583235444413 0.001359779948753436 84-85 0.0 0.0 2.2853444516864472E-5 5.822029257893808 0.0013712066710118683 86-87 0.0 0.0 2.2853444516864472E-5 6.350183787400805 0.0014397670045624617 88 0.0 0.0 2.2853444516864472E-5 6.770150110564964 0.001508327338113055 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 4695 0.0 38.00204 94 GTACTAG 5005 0.0 34.96023 1 TAGGCAT 5785 0.0 33.86609 5 TACCCCG 280 0.0 33.558636 5 CAACGCA 9620 0.0 31.353952 5 GCATCGT 5305 0.0 31.24294 8 GGTATCA 6835 0.0 31.120207 1 GTATCAA 10520 0.0 30.709965 1 CTAGGCA 6760 0.0 29.817616 4 TATTCTC 2540 0.0 28.855145 5 ATCAACG 10375 0.0 28.713654 3 TAGTACT 6680 0.0 28.345943 4 CTGTGCG 6690 0.0 28.215158 9 AGGGCTA 5545 0.0 27.19793 5 TACTGTG 7755 0.0 26.457405 7 GTACTGT 7275 0.0 26.136036 6 AACGCAG 12245 0.0 26.071606 6 ACTGTGC 7530 0.0 26.001818 8 CTATTGA 5740 0.0 25.768032 9 TATCAAC 12545 0.0 25.733175 2 >>END_MODULE