##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146476_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4375708
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.84606011187218	31.0	31.0	34.0	30.0	34.0
2	31.814608287390293	31.0	31.0	34.0	30.0	34.0
3	31.980098534911377	33.0	31.0	34.0	30.0	34.0
4	35.72604707626743	37.0	35.0	37.0	35.0	37.0
5	35.64308198810341	37.0	35.0	37.0	33.0	37.0
6	35.6810733257338	37.0	35.0	37.0	33.0	37.0
7	35.600486138471766	37.0	35.0	37.0	33.0	37.0
8	35.588264573413035	37.0	35.0	37.0	33.0	37.0
9	37.16729361282791	39.0	37.0	39.0	33.0	39.0
10-11	37.11453346521294	39.0	37.0	39.0	33.0	39.0
12-13	37.04233234941637	39.0	37.0	39.0	33.0	39.0
14-15	38.23122486692439	40.0	37.0	41.0	33.0	41.0
16-17	38.23847672193848	40.0	37.0	41.0	33.0	41.0
18-19	38.29287637566309	40.0	38.0	41.0	33.0	41.0
20-21	38.302149275043035	40.0	38.0	41.0	33.0	41.0
22-23	38.26916764098519	40.0	37.5	41.0	33.0	41.0
24-25	38.196459521522	40.0	37.0	41.0	33.0	41.0
26-27	38.045854979354196	40.0	37.0	41.0	33.0	41.0
28-29	37.98254293933691	40.0	37.0	41.0	33.0	41.0
30-31	37.850695018954646	40.0	37.0	41.0	33.0	41.0
32-33	37.72155477467875	40.0	36.0	41.0	32.5	41.0
34-35	37.62437712936969	40.0	36.0	41.0	32.0	41.0
36-37	37.53885931145314	40.0	36.0	41.0	32.0	41.0
38-39	37.41739062569989	40.0	36.0	41.0	31.5	41.0
40-41	37.28552190868312	39.5	36.0	41.0	31.0	41.0
42-43	37.15231592236045	39.0	35.0	41.0	31.0	41.0
44-45	36.93391846530893	39.0	35.0	41.0	31.0	41.0
46-47	36.67397024664351	39.0	35.0	41.0	30.0	41.0
48-49	36.38276548617961	39.0	35.0	41.0	30.0	41.0
50-51	36.16873463677193	38.5	35.0	41.0	29.0	41.0
52-53	35.969006958416784	38.0	35.0	40.0	29.0	41.0
54-55	35.75563006032395	38.0	34.5	40.0	29.0	41.0
56-57	35.47645718132928	37.0	34.0	40.0	28.0	41.0
58-59	35.178647318331116	37.0	34.0	40.0	28.0	41.0
60-61	34.91092904279719	36.0	34.0	39.5	27.5	41.0
62-63	34.62674371781664	36.0	34.0	39.0	27.0	41.0
64-65	34.347034925548044	35.0	33.0	39.0	26.5	41.0
66-67	34.12274927851676	35.0	33.0	39.0	26.0	41.0
68-69	33.93375654865453	35.0	33.0	38.0	26.0	40.5
70-71	33.73977434051815	35.0	33.0	37.0	26.0	40.0
72-73	33.55494996466857	35.0	33.0	37.0	26.0	40.0
74-75	33.37946259211081	35.0	33.0	36.0	26.0	39.0
76-77	33.234286200084654	35.0	33.0	36.0	26.0	39.0
78-79	33.13336527483095	35.0	33.0	36.0	26.5	39.0
80-81	33.006882429083475	35.0	33.0	35.5	26.5	37.5
82-83	32.82723173027085	35.0	33.0	35.0	26.0	37.0
84-85	32.69873606739755	35.0	33.0	35.0	26.0	37.0
86-87	32.57387935849467	35.0	33.0	35.0	26.0	36.0
88-89	32.47417960704873	35.0	33.0	35.0	26.0	36.0
90-91	32.39072225568982	35.0	33.0	35.0	26.0	36.0
92-93	32.31087426309068	35.0	33.0	35.0	26.0	36.0
94-95	32.27136271433103	35.0	33.0	35.0	26.0	35.0
96-97	32.22135914919369	35.0	33.0	35.0	25.0	35.0
98-99	32.18930742179323	35.0	33.0	35.0	25.0	35.0
100	31.692757149243047	34.0	32.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.47836571520023696
1101	2	0.5587186207965154
1101	3	0.46169676471998855
1101	4	0.251663159975557
1101	5	0.34775385963424554
1101	6	0.35648608719434804
1101	7	0.3980693032296614
1101	8	0.36695310751522214
1101	9	0.5099852501926918
1101	10-11	0.47491272768766635
1101	12-13	0.48746255054337695
1101	14-15	0.6452050999258674
1101	16-17	0.6201956626642939
1101	18-19	0.5995396457239082
1101	20-21	0.594599444011898
1101	22-23	0.5925987498613381
1101	24-25	0.6322228849531797
1101	26-27	0.6998839181461918
1101	28-29	0.7300666105948821
1101	30-31	0.7700172194599944
1101	32-33	0.7727291544907047
1101	34-35	0.8122225355450681
1101	36-37	0.8369239374164579
1101	38-39	0.8030371649927943
1101	40-41	0.7834469709881233
1101	42-43	0.7559964058871955
1101	44-45	0.7491919336619688
1101	46-47	0.7653686570070874
1101	48-49	0.7971148407187627
1101	50-51	0.8156705056677112
1101	52-53	0.7567094852657235
1101	54-55	0.714099895084324
1101	56-57	0.7588053202020149
1101	58-59	0.7878634870995143
1101	60-61	0.8165559605309411
1101	62-63	0.7630473743868009
1101	64-65	0.716155341801965
1101	66-67	0.7050164263745984
1101	68-69	0.6957889462416134
1101	70-71	0.6829799378127746
1101	72-73	0.5735005818236942
1101	74-75	0.5723924511201872
1101	76-77	0.6341827634576305
1101	78-79	0.5976665792412064
1101	80-81	0.5448944539945302
1101	82-83	0.5367287391824789
1101	84-85	0.51099882328392
1101	86-87	0.5541579418602183
1101	88-89	0.5043765012475703
1101	90-91	0.5188978003792002
1101	92-93	0.5503631272266105
1101	94-95	0.5778393616528419
1101	96-97	0.5855713326520657
1101	98-99	0.5728586452332181
1101	100	0.5366306749695937
1102	1	0.4397122898852537
1102	2	0.5488857905276099
1102	3	0.4475793468090288
1102	4	0.3395030148994991
1102	5	0.31779627744973027
1102	6	0.3425710832288189
1102	7	0.3858857978020396
1102	8	0.4220572806797165
1102	9	0.4877898927283866
1102	10-11	0.5058874717740593
1102	12-13	0.5239071655049443
1102	14-15	0.6798935511281883
1102	16-17	0.6674781758816692
1102	18-19	0.6561415547802127
1102	20-21	0.6225682137042909
1102	22-23	0.6466828967756726
1102	24-25	0.7006493011188013
1102	26-27	0.7400435132673735
1102	28-29	0.6777055283040667
1102	30-31	0.7649826499588173
1102	32-33	0.8074993987495986
1102	34-35	0.8001581392669408
1102	36-37	0.7753873927880051
1102	38-39	0.7807894536070634
1102	40-41	0.7620439240853756
1102	42-43	0.8352526046551461
1102	44-45	0.7803246942953805
1102	46-47	0.7853990445597816
1102	48-49	0.7583305306002259
1102	50-51	0.6265649588890909
1102	52-53	0.6788593632444915
1102	54-55	0.7163519889229235
1102	56-57	0.6966133967730457
1102	58-59	0.6738312358108729
1102	60-61	0.6419619182480716
1102	62-63	0.6253842497520026
1102	64-65	0.5808183862350091
1102	66-67	0.6321233773204042
1102	68-69	0.6467078609973314
1102	70-71	0.681562085411997
1102	72-73	0.6374127055278933
1102	74-75	0.6206889436302347
1102	76-77	0.6418817338199005
1102	78-79	0.5683693089014668
1102	80-81	0.5882963322431749
1102	82-83	0.5998850727657583
1102	84-85	0.5867605171449171
1102	86-87	0.5796160307161173
1102	88-89	0.5378051165961537
1102	90-91	0.5337159492718442
1102	92-93	0.5588761294582554
1102	94-95	0.5208572103617826
1102	96-97	0.5790667159591507
1102	98-99	0.6412627035691685
1102	100	0.6438643956052488
1103	1	0.297345420165005
1103	2	-0.14215263209109352
1103	3	0.23379859209663323
1103	4	0.2005278734156306
1103	5	0.19658562735371277
1103	6	0.24594892734335616
1103	7	0.2970180123552879
1103	8	0.3104213032257519
1103	9	0.37441325525872315
1103	10-11	0.37252161976945786
1103	12-13	0.3559601838124067
1103	14-15	0.48796797029322647
1103	16-17	0.5069780107045858
1103	18-19	0.4844702355955377
1103	20-21	0.45819526017307766
1103	22-23	0.4276709829933907
1103	24-25	0.42293898726965296
1103	26-27	0.3936552298162539
1103	28-29	0.47467866735579634
1103	30-31	0.5691328102494779
1103	32-33	0.5534884944331822
1103	34-35	0.5520871732276049
1103	36-37	0.4871815576673697
1103	38-39	0.5451415922876421
1103	40-41	0.5765856900441335
1103	42-43	0.6039255926541571
1103	44-45	0.5890230490337487
1103	46-47	0.651102960924689
1103	48-49	0.6405982428505084
1103	50-51	0.5831671738575395
1103	52-53	0.6067024986862535
1103	54-55	0.48641204058291976
1103	56-57	0.5719145480446173
1103	58-59	0.5438677869061621
1103	60-61	0.5467449120980774
1103	62-63	0.5774168978814913
1103	64-65	0.5226963154764874
1103	66-67	0.5548504427727821
1103	68-69	0.4993108641378754
1103	70-71	0.4948340638907496
1103	72-73	0.5565504444476588
1103	74-75	0.5725050234831102
1103	76-77	0.45502845830036165
1103	78-79	0.47958212887313323
1103	80-81	0.5019709094596578
1103	82-83	0.4671772547042252
1103	84-85	0.49969764487322266
1103	86-87	0.446423662241763
1103	88-89	0.4127353446910149
1103	90-91	0.41483607969826863
1103	92-93	0.442517510795561
1103	94-95	0.5185257272474644
1103	96-97	0.5128783013091933
1103	98-99	0.5244601941648668
1103	100	0.47714009374132615
1104	1	0.32413906371838763
1104	2	0.4158421893298829
1104	3	0.3322345922182137
1104	4	0.2297448099664834
1104	5	0.2462667731892978
1104	6	0.29828500645987077
1104	7	0.26459174247352735
1104	8	0.25558732366522463
1104	9	0.3279094970181191
1104	10-11	0.32069849212150814
1104	12-13	0.3816123738711994
1104	14-15	0.45553398356176444
1104	16-17	0.41851548281693596
1104	18-19	0.45041948460453085
1104	20-21	0.477076639118188
1104	22-23	0.45944849833088597
1104	24-25	0.46857882902099135
1104	26-27	0.46059418333459945
1104	28-29	0.4536299319789734
1104	30-31	0.5334279575776222
1104	32-33	0.5496793698009341
1104	34-35	0.5284172363015571
1104	36-37	0.5039430944082284
1104	38-39	0.5042699212068698
1104	40-41	0.46417966459308957
1104	42-43	0.4375372043495531
1104	44-45	0.4440057177980421
1104	46-47	0.45468527757149957
1104	48-49	0.46450172062912287
1104	50-51	0.4421143825853875
1104	52-53	0.45057425178803356
1104	54-55	0.44850717808471785
1104	56-57	0.4538204629998077
1104	58-59	0.4424190814801534
1104	60-61	0.4632158437361582
1104	62-63	0.4180879443489829
1104	64-65	0.3695106145765976
1104	66-67	0.32569636653393985
1104	68-69	0.32771272612945523
1104	70-71	0.31928004631345175
1104	72-73	0.2955541526466803
1104	74-75	0.3605148048091493
1104	76-77	0.35583202835092465
1104	78-79	0.2770498025179222
1104	80-81	0.3220325755133544
1104	82-83	0.269474193554629
1104	84-85	0.25748355870424433
1104	86-87	0.3225451412269038
1104	88-89	0.277764543026251
1104	90-91	0.32756875366892046
1104	92-93	0.2996522033862661
1104	94-95	0.3246019679703167
1104	96-97	0.3411219173346538
1104	98-99	0.33052315786244435
1104	100	0.39951754932546635
1105	1	0.04321167358163791
1105	2	-0.5149772658456939
1105	3	-0.08866173303058034
1105	4	0.02577971112661004
1105	5	0.09808386081492415
1105	6	0.12612393345008144
1105	7	0.15210160615928459
1105	8	0.16094556569934326
1105	9	0.17395512599386365
1105	10-11	0.228098568523194
1105	12-13	0.22687195980802954
1105	14-15	0.23540819677235447
1105	16-17	0.30820295374999773
1105	18-19	0.2688503760428844
1105	20-21	0.26671873742849783
1105	22-23	0.2782526076216527
1105	24-25	0.29878855682107996
1105	26-27	0.23103156772198474
1105	28-29	0.21212915502505325
1105	30-31	0.2370149954572227
1105	32-33	0.28476044143607737
1105	34-35	0.2956671591103799
1105	36-37	0.29339481877356377
1105	38-39	0.30522402672266224
1105	40-41	0.33843085709229825
1105	42-43	0.31580084536089004
1105	44-45	0.28095469651928084
1105	46-47	0.26156515774903255
1105	48-49	0.24719996329748284
1105	50-51	0.2668445319136623
1105	52-53	0.2456551270451044
1105	54-55	0.27364375040896505
1105	56-57	0.35549744548620055
1105	58-59	0.33033344418753074
1105	60-61	0.2715450405246287
1105	62-63	0.22113967948857294
1105	64-65	0.31172457565101297
1105	66-67	0.24751105781633242
1105	68-69	0.24708115036660416
1105	70-71	0.2144308082802695
1105	72-73	0.21027684226198318
1105	74-75	0.1842952737626362
1105	76-77	0.13812208990881203
1105	78-79	0.15569034347051058
1105	80-81	0.17878087574835888
1105	82-83	0.13175143215518403
1105	84-85	0.2188442159232764
1105	86-87	0.20163725222881368
1105	88-89	0.21408872102811216
1105	90-91	0.14868086922733426
1105	92-93	0.14393115949881263
1105	94-95	0.1450267934492615
1105	96-97	0.11636548917049083
1105	98-99	0.18131264415200832
1105	100	0.17481910576763937
1106	1	0.2531013522430676
1106	2	0.2533493562956757
1106	3	0.2279243459157243
1106	4	0.18467457994731262
1106	5	0.17460009142456556
1106	6	0.18069254475339847
1106	7	0.2549781185334581
1106	8	0.12183056665541869
1106	9	0.2402973876206076
1106	10-11	0.23323640755108954
1106	12-13	0.2195599373483077
1106	14-15	0.2924849985440261
1106	16-17	0.28545006523621197
1106	18-19	0.3122083849006074
1106	20-21	0.29497554380443347
1106	22-23	0.2721537437971193
1106	24-25	0.2596473270854318
1106	26-27	0.2830599384733574
1106	28-29	0.2423479107426587
1106	30-31	0.2571013756439129
1106	32-33	0.2681816671294186
1106	34-35	0.27170381537293053
1106	36-37	0.28302809329195355
1106	38-39	0.23597465314328048
1106	40-41	0.3136016291010719
1106	42-43	0.2799720873072715
1106	44-45	0.264610120164285
1106	46-47	0.2806521333811105
1106	48-49	0.21259670837263656
1106	50-51	0.2507039382722809
1106	52-53	0.28011185489399537
1106	54-55	0.3274908982620346
1106	56-57	0.2968049156180683
1106	58-59	0.2946847181414398
1106	60-61	0.24579458977147794
1106	62-63	0.24988388164798891
1106	64-65	0.23687620880905058
1106	66-67	0.18559004659363865
1106	68-69	0.2351578678439381
1106	70-71	0.2741614590302959
1106	72-73	0.2605839215825938
1106	74-75	0.16895557392773952
1106	76-77	0.13455196899444388
1106	78-79	0.15900776597847255
1106	80-81	0.14746192925458956
1106	82-83	0.17565751698515442
1106	84-85	0.15760335698276862
1106	86-87	0.16273884096187174
1106	88-89	0.19511697152652374
1106	90-91	0.11383578382753967
1106	92-93	0.13064446728849788
1106	94-95	0.14498533028216798
1106	96-97	0.1423849890840856
1106	98-99	0.22226895958407056
1106	100	0.24334722218118543
1107	1	0.027496588507034403
1107	2	-0.5901539125911128
1107	3	-0.05669118208104251
1107	4	0.01792927598992833
1107	5	0.0835737148749871
1107	6	0.0959468056722983
1107	7	0.11379404878050536
1107	8	0.13935880695919423
1107	9	0.12627682664133033
1107	10-11	0.15216520742405493
1107	12-13	0.1424717193922973
1107	14-15	0.1811579318988592
1107	16-17	0.23901100034939304
1107	18-19	0.22321485935538732
1107	20-21	0.15186573091943245
1107	22-23	0.22384237710474508
1107	24-25	0.19473509816342016
1107	26-27	0.19806022707582116
1107	28-29	0.13144637990980357
1107	30-31	0.18203381467495916
1107	32-33	0.21545773923074307
1107	34-35	0.2949505671794981
1107	36-37	0.2710551341098295
1107	38-39	0.22856565617210123
1107	40-41	0.26069225990670475
1107	42-43	0.23705781197819675
1107	44-45	0.20089216493175144
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2216	80-81	-0.6272440743830145
2216	82-83	-0.5080338551985761
2216	84-85	-0.5954807549782188
2216	86-87	-0.5603090620464499
2216	88-89	-0.6086110039761294
2216	90-91	-0.604831656647935
2216	92-93	-0.5163955850184792
2216	94-95	-0.5928742265615057
2216	96-97	-0.6133722200349538
2216	98-99	-0.5550734979322804
2216	100	-0.5706562446338452
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	7.0
13	54.0
14	228.0
15	786.0
16	2007.0
17	4076.0
18	7148.0
19	11238.0
20	16408.0
21	22879.0
22	31519.0
23	37712.0
24	39510.0
25	41060.0
26	44922.0
27	54647.0
28	68640.0
29	85899.0
30	105660.0
31	130462.0
32	162413.0
33	206799.0
34	282536.0
35	390911.0
36	580518.0
37	979556.0
38	774497.0
39	293178.0
40	436.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.11144811697024	22.014385858248783	13.792618733614773	24.081547291166206
2	14.937561136149636	20.21954162743288	40.26335396345087	24.57954327296662
3	18.029900560764066	26.950384595431476	29.741978139411472	25.277736704392982
4	12.333955043475896	17.304862529211956	38.9853178414047	31.375864585907443
5	12.64983088033641	38.88115915531584	34.92170216656002	13.547307797787733
6	29.329813598165146	39.11408165261484	17.667518033653067	13.888586715566944
7	25.906651086651227	32.592543669102774	23.93713358644383	17.563671657802164
8	26.532140627299626	34.71936427202181	19.526348650321275	19.222146450357293
9	26.239410856483108	14.468195775403661	21.776498797451747	37.51589457066148
10-11	25.90880150137989	25.91385211261812	26.927219549384922	21.25012683661707
12-13	26.241787614712862	23.757195407006133	27.613325660670228	22.387691317610773
14-15	23.56138653960255	24.573928696480536	25.502419935072236	26.362264828844673
16-17	21.71874351869934	27.065180216010788	27.023974141874362	24.192102123415506
18-19	21.17357922420783	27.04107540996794	29.763651962151037	22.021693403673186
20-21	22.817978578321334	26.22345072196896	28.902777436563117	22.05579326314659
22-23	23.334943372665602	25.73848413368514	28.705744343808416	22.22082814984084
24-25	22.740217126005664	25.92279923614647	29.448457255374443	21.88852638247342
26-27	22.58452878043028	26.17401242635748	28.379305797770826	22.862152995441416
28-29	21.649353658882173	26.812826632855757	29.02889086748933	22.50892884077274
30-31	22.677255886361706	26.222762122152577	28.82832903840933	22.271652953076394
32-33	23.18719622058876	26.55871918327274	27.854166685711203	22.399917910427295
34-35	23.02870909841337	26.3797950767034	28.75726228984438	21.834233535038848
36-37	22.788677855103675	25.8569813159379	28.479619755248752	22.874721073709672
38-39	22.94782924271912	25.87671526527821	29.163931871139482	22.011523620863183
40-41	22.36109935106242	25.109739822777684	29.770124280956345	22.75903654520355
42-43	22.646564177383294	25.368520117001324	28.69438260639439	23.29053309922099
44-45	22.79463117740032	26.385067285111347	28.346704121938664	22.47359741554967
46-47	22.916588584064566	26.174415660277152	27.53488121236609	23.374114543292194
48-49	23.880963524788353	25.865716080046	27.49326733831785	22.7600530568478
50-51	23.23412576890414	25.98322374344906	27.17620782739616	23.606442660250636
52-53	22.46955235587018	26.5706601080328	27.135208747932904	23.82457878816411
54-55	22.660755983195802	26.570022916372665	26.80798525033466	23.96123585009687
56-57	23.77030344949104	25.86522372916721	26.689365492885887	23.67510732845587
58-59	22.480508544976637	26.317129409823504	27.729097941940484	23.473264103259375
60-61	22.639097315643685	27.017078049946015	26.854235700788298	23.489588933622002
62-63	23.207021583707142	26.132216774976758	26.352798221453533	24.30796341986257
64-65	22.15807133382758	26.3471991275469	26.914810129012267	24.579919409613254
66-67	22.936562494572307	25.95072614534608	26.861687297232812	24.2510240628488
68-69	22.417898502417128	25.867796586153695	27.23664433018081	24.47766058124837
70-71	22.388878509531963	25.491235918593087	27.42738401925647	24.692501552618484
72-73	23.085258431321286	25.60778735692601	27.73183219721243	23.575122014540277
74-75	23.219185133900275	26.126513914743477	27.151206989600862	23.50309396175538
76-77	22.85769004909399	26.11507051683243	26.606056125276005	24.421183308797577
78-79	22.554297212893523	26.141906924287284	26.882042468779172	24.421753394040017
80-81	22.149267900928997	26.614527162067382	27.528545430320033	23.707659506683587
82-83	23.12413469682336	25.451991642811077	28.56231805493787	22.861555605427693
84-85	23.448965916331694	26.065314613202517	26.9119440480424	23.573775422423385
86-87	21.932465140091008	26.80933269643482	27.42480064543113	23.833401518043047
88-89	22.88130199032384	25.434420926940625	27.26671326818948	24.41756381454605
90-91	22.42662410189217	25.45163936318307	27.626958970382237	24.49477756454252
92-93	22.84855217487744	25.850022803949912	27.17829717632133	24.123127844851318
94-95	21.52296650242039	25.723913357220862	27.987294214600844	24.76582592575791
96-97	21.91890304125308	25.573181034326126	27.51243824870464	24.995477675716153
98-99	21.195207268628536	26.473469167611675	27.375580088426204	24.955743475333588
100	22.095977241137497	25.57012089616452	26.500383454263044	25.83351840843494
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	232.0
1	163.0
2	151.5
3	202.0
4	142.5
5	114.0
6	212.0
7	552.5
8	979.0
9	1148.5
10	951.5
11	682.5
12	622.0
13	717.0
14	869.0
15	1180.5
16	1577.5
17	2077.0
18	2864.0
19	3722.5
20	4625.0
21	5581.5
22	6607.0
23	7540.0
24	8295.0
25	9201.0
26	10536.0
27	11992.0
28	14995.0
29	22850.0
30	34484.0
31	44390.0
32	59292.0
33	73998.0
34	83094.0
35	95420.0
36	107900.0
37	131382.0
38	141893.5
39	129891.0
40	117270.0
41	104912.0
42	102297.5
43	109920.5
44	121863.0
45	151205.0
46	181420.0
47	200527.5
48	213309.0
49	207527.0
50	195746.5
51	199823.0
52	201351.0
53	193782.5
54	188465.0
55	164601.0
56	143753.5
57	119600.5
58	94014.5
59	91464.0
60	75930.5
61	51707.0
62	38017.5
63	30738.0
64	22067.0
65	10386.0
66	5572.5
67	4019.5
68	2953.5
69	2167.0
70	1732.5
71	1315.5
72	921.0
73	705.5
74	567.0
75	443.0
76	281.5
77	146.0
78	84.0
79	44.0
80	23.5
81	11.5
82	5.5
83	1.5
84	0.5
85	0.5
86	0.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.013209290930747665
2	0.005210585349845099
3	0.0016225945606973774
4	0.027766935087990333
5	0.0024681720078213627
6	0.0
7	3.8850855678669604E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008775722694475957
16-17	0.0031880555101025934
18-19	0.0
20-21	1.1426722258432236E-5
22-23	8.798576138992822E-4
24-25	0.0
26-27	2.0568100065178024E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	5.027757793710184E-4
36-37	0.0
38-39	0.0
40-41	3.9993527904512823E-4
42-43	7.084567800227985E-4
44-45	0.0
46-47	0.0
48-49	5.484826684047473E-4
50-51	0.0
52-53	0.0
54-55	3.5422839001139926E-4
56-57	1.0284050032589012E-4
58-59	1.9425427839334802E-4
60-61	7.998705580902565E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0013026463374612748
70-71	8.112972803486887E-4
72-73	0.0
74-75	1.7140083387648353E-4
76-77	0.002776693508799033
78-79	5.256292238878828E-4
80-81	0.0031766287878441614
82-83	0.001691154894247971
84-85	0.005484826684047473
86-87	0.007724464246700192
88-89	0.007187408300553876
90-91	0.005919042129867898
92-93	0.008444347748981423
94-95	0.001451193726820894
96-97	0.004399288069496411
98-99	0.0018739824503828867
100	0.08247808126136387
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4375708.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.37279676159129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	65.53835785813116	12.041229290158833
2	15.500123859868498	5.695612509137117
3	6.572569184955274	3.6226943351004284
4	3.4092305139352646	2.5054839738379213
5	2.027060628897855	1.862138647908186
6	1.3088017894302164	1.442780956704502
7	0.8909939033682439	1.1459034931681158
8	0.639949477145168	0.9406129345019826
9	0.4892106280921935	0.8089350699193466
>10	2.714837253607553	9.502416104211168
>50	0.3419582260095471	4.460725324784689
>100	0.4125757045113743	17.027464186345505
>500	0.08332131448182176	10.800572513739501
>1k	0.06665705158545739	21.729370480874046
>5k	0.003482084784314938	4.122749926077874
>10k+	8.705211960787345E-4	2.2913102535309298
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	19197	0.4387175743902473	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	16079	0.3674605343866638	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	14544	0.3323804970532769	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	13570	0.3101212420938509	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	13193	0.301505493510993	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	13053	0.29830601127863193	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	10647	0.24332062377105604	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9397	0.21475381812497543	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8965	0.20488113009368997	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	8104	0.1852043143646697	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	8089	0.1848615126969167	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	7403	0.1691840497583477	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	7073	0.1616424130677824	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	7023	0.1604997408419392	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	6976	0.15942562894964654	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	6945	0.15871717216962375	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6647	0.15190684570359814	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	6505	0.14866165658220337	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	6467	0.14779322569056255	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	6411	0.14651343279761814	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	6251	0.1428568816749198	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	6182	0.14127999400325614	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	6110	0.13963454599804193	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	6059	0.13846902032768185	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5896	0.13474390887143292	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5805	0.13266424542039826	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	5721	0.13074455608098162	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	5648	0.12907625463125055	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	5522	0.1261967206221256	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	5516	0.12605959995502442	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	5289	0.12087186804969619	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	5238	0.1197063423793361	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5149	0.11767238581733516	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	5032	0.11499853280886202	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	5016	0.11463287769659218	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	4984	0.11390156747205253	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	4920	0.1124389470229732	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	4883	0.11159336957584923	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	4875	0.11141054201971429	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	4771	0.10903378378996038	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	4667	0.10665702556020648	No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC	4642	0.10608568944728486	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	4603	0.10519440511112717	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	4545	0.10386890532914901	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	4468	0.10210919010135044	No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT	4440	0.10146929365487825	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	4407	0.10071512998582172	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.856033355059342E-5
2	0.0	0.0	0.0	0.0	6.856033355059342E-5
3	0.0	0.0	0.0	0.0	6.856033355059342E-5
4	0.0	0.0	0.0	0.0	6.856033355059342E-5
5	0.0	0.0	0.0	0.0	6.856033355059342E-5
6	0.0	0.0	0.0	0.0	6.856033355059342E-5
7	0.0	0.0	0.0	0.0	6.856033355059342E-5
8	0.0	0.0	0.0	0.0	6.856033355059342E-5
9	0.0	0.0	0.0	0.0	6.856033355059342E-5
10-11	0.0	0.0	0.0	0.0	6.856033355059342E-5
12-13	0.0	0.0	0.0	0.0	2.1710772291021248E-4
14-15	0.0	0.0	0.0	0.0	2.8566805646080586E-4
16-17	0.0	0.0	0.0	0.0	3.770818345282638E-4
18-19	0.0	0.0	0.0	0.0	3.88508556786696E-4
20-21	0.0	0.0	0.0	0.0	4.4564216807885715E-4
22-23	0.0	0.0	0.0	4.5706889033728944E-5	4.570688903372894E-4
24-25	0.0	0.0	0.0	1.7140083387648353E-4	4.799223348541539E-4
26-27	0.0	0.0	0.0	4.3421544582042496E-4	5.256292238878828E-4
28-29	0.0	0.0	0.0	0.0015197540603714872	5.256292238878828E-4
30-31	0.0	0.0	0.0	0.005633374073407092	5.256292238878828E-4
32-33	0.0	0.0	0.0	0.015848863772445508	5.256292238878828E-4
34-35	0.0	0.0	0.0	0.03167487410037416	5.713361129216118E-4
36-37	0.0	0.0	0.0	0.061201524416163056	5.713361129216118E-4
38-39	0.0	0.0	0.0	0.10848530112155563	5.941895574384762E-4
40-41	0.0	0.0	0.0	0.16343640846235627	6.170430019553407E-4
42-43	0.0	0.0	0.0	0.22142702392389985	6.170430019553407E-4
44-45	0.0	0.0	0.0	0.2942380981546301	6.513231687306374E-4
46-47	0.0	0.0	0.0	0.3879715008405497	7.084567800227986E-4
48-49	0.0	0.0	0.0	0.5004790081970735	7.427369467980954E-4
50-51	0.0	0.0	0.0	0.6191683722954091	7.77017113573392E-4
52-53	0.0	0.0	0.0	0.7364293961114408	8.455774471239854E-4
54-55	0.0	0.0	0.0	0.8678709822501867	8.455774471239854E-4
56-57	0.0	0.0	0.0	1.0310560028228575	8.570041693824177E-4
58-59	0.0	0.0	0.0	1.2326233834616023	8.684308916408499E-4
60-61	0.0	0.0	0.0	1.4442348529655087	8.798576138992822E-4
62-63	0.0	0.0	0.0	1.667341605061398	9.826981142251722E-4
64-65	0.0	0.0	0.0	1.9049602944254964	0.0010512584477757657
66-67	0.0	0.0	0.0	2.1770076979542514	0.0010512584477757657
68-69	0.0	0.0	0.0	2.4837809104263813	0.0010512584477757657
70-71	0.0	0.0	0.0	2.8278737977945516	0.001062685170034198
72-73	0.0	0.0	0.0	3.1887868203271337	0.0011312455035847914
74-75	0.0	0.0	0.0	3.5419982320575323	0.0011883791148769524
76-77	0.0	0.0	0.0	3.9605476416616465	0.0011883791148769524
78-79	0.0	0.0	0.0	4.410109175475146	0.0011883791148769524
80-81	0.0	0.0	0.0	4.906268882658532	0.0012112325593938169
82-83	0.0	0.0	0.0	5.416837686609801	0.0013254997819781393
84-85	0.0	0.0	0.0	5.921190353652483	0.0013254997819781393
86-87	0.0	0.0	0.0	6.4604173770279	0.001359779948753436
88	0.0	0.0	0.0	6.889147996164278	0.0013940601155287328
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	10140	0.0	44.79561	1
TACCCCG	330	0.0	39.876953	5
GTATCAA	16885	0.0	36.843006	1
GGGCACC	4675	0.0	36.423122	94
GTGGTAT	2615	0.0	33.98493	1
CAGTACG	820	0.0	32.10232	9
TATTCTC	2750	0.0	31.616724	5
CAACGCA	19330	0.0	30.8051	5
ATCAACG	19870	0.0	30.047462	3
GTACTAG	4895	0.0	29.970787	1
TAGGCAT	5915	0.0	29.001198	5
TATCAAC	21745	0.0	28.625648	2
AACGCAG	22190	0.0	27.702461	6
CTGTGCG	6600	0.0	27.349508	9
TATAGAC	1380	0.0	26.912245	3
AGGGCTA	6245	0.0	26.7914	5
TAGTACT	6775	0.0	26.645193	4
ACGCAGA	23140	0.0	25.732452	7
CTATTGA	6450	0.0	25.653414	9
CTAGGCA	6875	0.0	25.642216	4
>>END_MODULE