##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1146475_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3794592 Sequences flagged as poor quality 0 Sequence length 100 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.716872591308896 31.0 30.0 34.0 25.0 34.0 2 29.685533517173916 31.0 30.0 34.0 25.0 34.0 3 29.97716328922846 31.0 30.0 34.0 25.0 34.0 4 33.33173395189786 35.0 33.0 37.0 26.0 37.0 5 33.59642380524705 35.0 33.0 37.0 27.0 37.0 6 33.772851732149334 36.0 35.0 37.0 28.0 37.0 7 33.57935978360783 35.0 33.0 37.0 27.0 37.0 8 33.55812588020003 35.0 33.0 37.0 27.0 37.0 9 34.76976971437245 38.0 34.0 39.0 27.0 39.0 10-11 34.708215797640435 38.0 34.0 39.0 27.0 39.0 12-13 34.7098199226689 38.0 34.0 39.0 27.0 39.0 14-15 35.599245320709045 39.0 34.0 41.0 26.0 41.0 16-17 35.41839491571163 39.0 34.0 41.0 25.0 41.0 18-19 35.365033184068274 38.5 33.5 41.0 25.0 41.0 20-21 35.713563539900996 39.0 34.0 41.0 26.0 41.0 22-23 35.41739704822021 39.0 34.0 41.0 25.0 41.0 24-25 35.51611424891003 39.0 34.0 41.0 25.5 41.0 26-27 35.592447356659164 39.0 34.0 41.0 25.5 41.0 28-29 35.45281798412056 39.0 34.0 41.0 25.5 41.0 30-31 35.401446716801175 38.0 33.5 41.0 25.5 41.0 32-33 35.51474848942917 38.0 34.0 41.0 26.0 41.0 34-35 35.554951889425794 38.0 34.0 41.0 26.0 41.0 36-37 35.46799445105033 38.0 33.0 40.0 26.0 41.0 38-39 35.28776637909952 38.0 33.0 40.0 25.0 41.0 40-41 35.239037293073935 38.0 33.0 40.0 25.5 41.0 42-43 35.04532384509323 38.0 33.0 40.0 25.0 41.0 44-45 34.80443668252081 38.0 33.0 40.0 24.5 41.0 46-47 34.66567868166064 38.0 33.0 40.0 24.5 41.0 48-49 34.52219751161653 37.5 32.0 40.0 24.0 41.0 50-51 34.282028212782826 37.0 32.0 40.0 24.0 41.0 52-53 34.06930837360117 37.0 32.0 40.0 24.0 41.0 54-55 33.87475965795532 36.0 32.0 40.0 23.0 41.0 56-57 33.69450681390779 36.0 31.0 39.5 23.0 41.0 58-59 33.42387099851578 35.0 31.0 39.0 23.0 41.0 60-61 33.17736926657727 35.0 31.0 39.0 23.0 40.5 62-63 32.89993996719542 35.0 31.0 38.5 23.0 40.0 64-65 32.61192191413464 35.0 30.0 38.0 22.0 40.0 66-67 32.34474694512612 35.0 30.0 37.0 22.0 40.0 68-69 32.06193762069809 34.5 30.0 37.0 21.5 40.0 70-71 32.05439952437574 34.5 30.0 36.5 22.0 40.0 72-73 31.89176056345452 34.0 30.0 36.0 22.0 39.0 74-75 31.660331202933016 34.0 30.0 36.0 21.0 39.0 76-77 31.46179641974684 34.0 30.0 35.0 21.0 39.0 78-79 31.270590751258634 34.0 29.0 35.0 20.0 38.0 80-81 31.09037519712264 34.0 29.0 35.0 20.0 37.0 82-83 30.862294022651184 34.0 29.0 35.0 20.0 37.0 84-85 30.69664077191962 34.0 29.0 35.0 20.0 36.0 86-87 30.561291042620656 34.0 29.0 35.0 20.0 36.0 88-89 30.61828768415682 34.0 29.0 35.0 20.0 36.0 90-91 30.553336696013695 34.0 29.0 35.0 20.0 36.0 92-93 30.41144528845262 34.0 29.0 35.0 19.5 35.0 94-95 30.40552343967415 34.0 29.0 35.0 20.0 35.0 96-97 30.325041137492516 34.0 29.0 35.0 19.0 35.0 98-99 30.25168384374394 34.0 29.0 35.0 19.0 35.0 100 29.687153454179 34.0 28.0 35.0 18.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 1.2215897488150276 1101 2 1.4230250874493606 1101 3 1.4127859933746585 1101 4 1.5017829783921925 1101 5 1.4240409632215574 1101 6 1.17894471344286 1101 7 1.2428236593946593 1101 8 1.2248404772319077 1101 9 1.486687087444615 1101 10-11 1.4611414216225924 1101 12-13 1.3739728502069468 1101 14-15 1.6422746453820167 1101 16-17 1.8376824080891367 1101 18-19 1.9936836201402741 1101 20-21 1.6144690366332668 1101 22-23 1.987732129260415 1101 24-25 1.7984484473423805 1101 26-27 1.7068762785631009 1101 28-29 1.9095166445604548 1101 30-31 1.9134206026715574 1101 32-33 1.7426634523286708 1101 34-35 1.6629823795353218 1101 36-37 1.5989119824029956 1101 38-39 1.738159438260496 1101 40-41 1.6112626792322047 1101 42-43 1.6510206545206714 1101 44-45 1.6684859007742645 1101 46-47 1.569163253722465 1101 48-49 1.6213177871691826 1101 50-51 1.6416339848712411 1101 52-53 1.665567843393383 1101 54-55 1.6428707486625953 1101 56-57 1.497317413937452 1101 58-59 1.4478405486172363 1101 60-61 1.520088084879994 1101 62-63 1.435691849420607 1101 64-65 1.3770312144840418 1101 66-67 1.3665324263124177 1101 68-69 1.43440826165088 1101 70-71 1.294194708263685 1101 72-73 1.3273358459286904 1101 74-75 1.2712528042592375 1101 76-77 1.3301188672929847 1101 78-79 1.3134587903534296 1101 80-81 1.4077254973467 1101 82-83 1.2961303547414218 1101 84-85 1.3392591361287707 1101 86-87 1.3132771330959585 1101 88-89 1.2974959580513499 1101 90-91 1.3570928336425716 1101 92-93 1.3546340215737729 1101 94-95 1.294265545564265 1101 96-97 1.2769639414070895 1101 98-99 1.318273403404202 1101 100 1.358719560117244 1102 1 1.2282512138020536 1102 2 1.3156176513183269 1102 3 1.3283541460986505 1102 4 1.5145288676231843 1102 5 1.3319900390718473 1102 6 1.0373229025192998 1102 7 1.22089405826393 1102 8 1.1191013853117653 1102 9 1.4330272151488401 1102 10-11 1.3947116082834086 1102 12-13 1.318340374471525 1102 14-15 1.5870846824441145 1102 16-17 1.8550792183372664 1102 18-19 1.8404021545784346 1102 20-21 1.6015529219037319 1102 22-23 1.8639467267793108 1102 24-25 1.6977448266499522 1102 26-27 1.5505022796215897 1102 28-29 1.776331900232229 1102 30-31 1.8153760885199333 1102 32-33 1.657526423146706 1102 34-35 1.4742217027735904 1102 36-37 1.459310118212663 1102 38-39 1.5189175625394853 1102 40-41 1.4915692782537207 1102 42-43 1.4810771121585802 1102 44-45 1.5227152916024238 1102 46-47 1.4973170431906908 1102 48-49 1.2958755392203898 1102 50-51 1.4355800423077767 1102 52-53 1.4087318823805361 1102 54-55 1.4634406037682979 1102 56-57 1.3860333147908648 1102 58-59 1.3730124962660994 1102 60-61 1.297842381093794 1102 62-63 1.3712897888823221 1102 64-65 1.2230557565580682 1102 66-67 1.2941451893952802 1102 68-69 1.2720841763281285 1102 70-71 1.225494634465928 1102 72-73 1.185988514592598 1102 74-75 1.239184363090903 1102 76-77 1.197762972751331 1102 78-79 1.2388512594141545 1102 80-81 1.2762804796631606 1102 82-83 1.2852985234425063 1102 84-85 1.2011121784904262 1102 86-87 1.155013571561362 1102 88-89 1.174488260406914 1102 90-91 1.0931586353462528 1102 92-93 1.1894877513255828 1102 94-95 1.2053014269653595 1102 96-97 1.20488212955998 1102 98-99 1.2605169549460236 1102 100 1.312246440336633 1103 1 0.9111741431272833 1103 2 1.1302962570106843 1103 3 1.0761955276144803 1103 4 1.1846599837088831 1103 5 1.0724761279749515 1103 6 0.9498588794940375 1103 7 0.9530654685613413 1103 8 0.9852670392932126 1103 9 1.1954192842246343 1103 10-11 1.2130678602821305 1103 12-13 1.1536457790126136 1103 14-15 1.324772562201069 1103 16-17 1.5511937050657636 1103 18-19 1.6631555923782457 1103 20-21 1.305982087237922 1103 22-23 1.5907615652826692 1103 24-25 1.4866799081977646 1103 26-27 1.3352800218665521 1103 28-29 1.5357658624963832 1103 30-31 1.5907198685007486 1103 32-33 1.3787448433577723 1103 34-35 1.307768232994249 1103 36-37 1.2308963030543651 1103 38-39 1.428175637371588 1103 40-41 1.2826629782856998 1103 42-43 1.3270956602877746 1103 44-45 1.3574610543142924 1103 46-47 1.3470931864589986 1103 48-49 1.3512800803140905 1103 50-51 1.3896094634782301 1103 52-53 1.4075326498893261 1103 54-55 1.3671022603081084 1103 56-57 1.2601139032142754 1103 58-59 1.2904253520384046 1103 60-61 1.2936850417909582 1103 62-63 1.1647819538967141 1103 64-65 1.2052271668554795 1103 66-67 1.13944419802975 1103 68-69 1.272244073993619 1103 70-71 1.1952739756875523 1103 72-73 1.1621637624339805 1103 74-75 1.1706682134266835 1103 76-77 1.1054220709285083 1103 78-79 1.1593021708308484 1103 80-81 1.243901202400611 1103 82-83 1.152823307580654 1103 84-85 1.133875210150162 1103 86-87 1.171483031107254 1103 88-89 1.0492883883250421 1103 90-91 1.0327972666222323 1103 92-93 1.0445405175551947 1103 94-95 1.0515598215928001 1103 96-97 1.0290746463492368 1103 98-99 1.1061357589114849 1103 100 1.1261255577015206 1104 1 0.8718914881661348 1104 2 1.0827861357587878 1104 3 1.0753371229722788 1104 4 1.2758841409339965 1104 5 1.0869310638913348 1104 6 0.9153214385044635 1104 7 0.9031547601270162 1104 8 0.9022010989511884 1104 9 1.0456057068638955 1104 10-11 1.1426049331710573 1104 12-13 1.0427658810318192 1104 14-15 1.2177157191241648 1104 16-17 1.3594552200346257 1104 18-19 1.3616649060319759 1104 20-21 1.284934132743146 1104 22-23 1.468040890208826 1104 24-25 1.270097017061019 1104 26-27 1.258986362843558 1104 28-29 1.4750483178696783 1104 30-31 1.4930925538058233 1104 32-33 1.2927903993117695 1104 34-35 1.200580407227072 1104 36-37 1.2278535103563684 1104 38-39 1.3247745112008857 1104 40-41 1.1895710450357626 1104 42-43 1.0529196713943705 1104 44-45 1.1917280840725368 1104 46-47 1.148305510903164 1104 48-49 1.0768437682187084 1104 50-51 1.238204539276893 1104 52-53 1.356650858097666 1104 54-55 1.111695703124397 1104 56-57 1.1498962074313397 1104 58-59 1.1071634138927493 1104 60-61 1.081834138772109 1104 62-63 1.078606598934762 1104 64-65 1.1064491902589992 1104 66-67 1.2211746808509716 1104 68-69 1.1589116201942957 1104 70-71 1.008936388640084 1104 72-73 0.9751898059209942 1104 74-75 1.0301293196396166 1104 76-77 1.0941777457292368 1104 78-79 0.9820245840875685 1104 80-81 1.0159320007778128 1104 82-83 0.9926782668311311 1104 84-85 0.9826019634069674 1104 86-87 0.8484201437015351 1104 88-89 0.8827120709554883 1104 90-91 0.9458340954328968 1104 92-93 0.9628559020450354 1104 94-95 0.8958976631021507 1104 96-97 0.9214101214034578 1104 98-99 0.9772075351014387 1104 100 1.026189238860571 1105 1 0.8625061738587974 1105 2 1.022222749216759 1105 3 1.0699088077551373 1105 4 1.1700169711413793 1105 5 1.1335435417655333 1105 6 0.829821091074507 1105 7 0.8937486866844253 1105 8 0.9496466240377472 1105 9 1.0085586958404207 1105 10-11 1.129057958538148 1105 12-13 1.0772316823057722 1105 14-15 1.368144471003255 1105 16-17 1.4415265605908019 1105 18-19 1.3916522839314496 1105 20-21 1.2059209778330882 1105 22-23 1.49226775706407 1105 24-25 1.3847162854569461 1105 26-27 1.2229178867997348 1105 28-29 1.547356483160172 1105 30-31 1.5182073703232533 1105 32-33 1.3093748004555437 1105 34-35 1.1943412317441613 1105 36-37 1.0787864931880904 1105 38-39 1.2237722546878445 1105 40-41 1.1998540429538593 1105 42-43 1.1630151492625842 1105 44-45 1.2125562136543309 1105 46-47 1.144183941036765 1105 48-49 1.1835941696882202 1105 50-51 1.2586191498642023 1105 52-53 1.249142239145577 1105 54-55 1.165654257908166 1105 56-57 1.1567488451588304 1105 58-59 1.0583476865533683 1105 60-61 1.0588174723897623 1105 62-63 1.039875428569232 1105 64-65 1.0170222871596835 1105 66-67 1.1071788569632304 1105 68-69 1.0522741648093614 1105 70-71 1.042791322561353 1105 72-73 0.8985322665693296 1105 74-75 0.8846127850504679 1105 76-77 0.8976762038295725 1105 78-79 1.040789114582104 1105 80-81 1.1237973490804727 1105 82-83 1.0904443082480384 1105 84-85 1.0430679111371042 1105 86-87 0.9910995923339385 1105 88-89 0.9163154617665477 1105 90-91 1.0319926142258886 1105 92-93 0.9801138204197706 1105 94-95 0.8747967594763644 1105 96-97 1.0035456886382654 1105 98-99 1.0401992438382592 1105 100 1.1177203633970834 1106 1 0.7454813103439086 1106 2 0.8747546107035298 1106 3 0.8745284352451677 1106 4 1.1035302580762973 1106 5 0.9119300846054159 1106 6 0.6333772659483827 1106 7 0.7168419186909958 1106 8 0.8403514246814439 1106 9 1.0994449930442158 1106 10-11 0.9378090151372191 1106 12-13 0.9332712028105448 1106 14-15 1.0591112300970238 1106 16-17 1.1843729100164637 1106 18-19 1.0820994226337604 1106 20-21 0.9522749741465901 1106 22-23 1.2596735542309219 1106 24-25 1.0884938457649085 1106 26-27 0.9865845083906635 1106 28-29 1.2203011714454348 1106 30-31 1.2190951828287595 1106 32-33 1.0505040869386448 1106 34-35 0.9544179153266015 1106 36-37 0.8968008928726618 1106 38-39 1.0452223329202113 1106 40-41 0.9653241671285429 1106 42-43 1.019962383041225 1106 44-45 1.0590953674153454 1106 46-47 0.9834665681924335 1106 48-49 1.0304953485812973 1106 50-51 1.0304303847079694 1106 52-53 0.9828925084961568 1106 54-55 1.05128492060836 1106 56-57 1.0340488904723202 1106 58-59 0.9318934663507719 1106 60-61 0.8808062153914378 1106 62-63 0.8985990661162404 1106 64-65 0.8471122295107705 1106 66-67 0.8372078670092975 1106 68-69 0.8736334607971799 1106 70-71 0.9015998956075997 1106 72-73 0.8077020350435511 1106 74-75 0.8557934011052772 1106 76-77 0.9228646255077102 1106 78-79 0.9172187590461292 1106 80-81 0.936505863790277 1106 82-83 0.8654136467996807 1106 84-85 0.7961063991697408 1106 86-87 0.7632655499432381 1106 88-89 0.850959523799883 1106 90-91 0.6872043059503454 1106 92-93 0.8381260837375741 1106 94-95 0.855702829839057 1106 96-97 0.8805667398811288 1106 98-99 0.8007533833169553 1106 100 0.7581347797339646 1107 1 0.6805063405614113 1107 2 0.8121416184894592 1107 3 0.8230828573752547 1107 4 1.0214399942996764 1107 5 0.892981741224574 1107 6 0.6690435697133523 1107 7 0.636867692627817 1107 8 0.7466213660463623 1107 9 0.8327190204905861 1107 10-11 0.7934680317027798 1107 12-13 0.7017460782219374 1107 14-15 1.0354607306801213 1107 16-17 1.1176887482381375 1107 18-19 1.2178797591948793 1107 20-21 0.9245252188239945 1107 22-23 1.2276743491981321 1107 24-25 0.9146950149666822 1107 26-27 0.852820951137403 1107 28-29 1.0950915863408213 1107 30-31 1.0494961787876775 1107 32-33 0.9864694283683093 1107 34-35 0.8679430199160407 1107 36-37 0.8493208514243804 1107 38-39 0.8748879342141578 1107 40-41 0.7325320823020434 1107 42-43 0.9329969328660397 1107 44-45 0.8457622549062336 1107 46-47 0.829661712642725 1107 48-49 0.7855509275386652 1107 50-51 0.8886531330196021 1107 52-53 0.9074720570897696 1107 54-55 0.8616166569242338 1107 56-57 0.7062005017183566 1107 58-59 0.7160066091836299 1107 60-61 0.8725418187977994 1107 62-63 0.8028254667772146 1107 64-65 0.8923820371312132 1107 66-67 0.8706042394089337 1107 68-69 0.9017754249411638 1107 70-71 0.7362642407033668 1107 72-73 0.7013287729406166 1107 74-75 0.6808839911184386 1107 76-77 0.6133120820501325 1107 78-79 0.6239179827031549 1107 80-81 0.7756249251289979 1107 82-83 0.7280288764214546 1107 84-85 0.6459470307436881 1107 86-87 0.8304974141288426 1107 88-89 0.8190539149062523 1107 90-91 0.830702374155031 1107 92-93 0.8028651007531202 1107 94-95 0.7384212052060697 1107 96-97 0.8081316903269204 1107 98-99 0.7705502291970596 1107 100 0.7454103296175347 1108 1 0.3747226195554525 1108 2 0.5698480301398767 1108 3 0.5558955594931447 1108 4 0.7509641820451307 1108 5 0.6127951526904738 1108 6 0.31419865898202204 1108 7 0.26237528507770236 1108 8 0.36428608514518857 1108 9 0.4291935545140717 1108 10-11 0.49993216493506765 1108 12-13 0.38380816756147595 1108 14-15 0.5924948770714238 1108 16-17 0.6971809418984378 1108 18-19 0.8257488611832855 1108 20-21 0.48945685389349336 1108 22-23 0.6705737373455705 1108 24-25 0.5214577639168638 1108 26-27 0.47386690424637123 1108 28-29 0.7097216495436598 1108 30-31 0.6835183862965479 1108 32-33 0.5900543237889551 1108 34-35 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2215 12-13 -2.2615166715591215 2215 14-15 -4.557171642262734 2215 16-17 -4.362142591240193 2215 18-19 -3.4561477749806713 2215 20-21 -2.826519861857861 2215 22-23 -4.295634404681529 2215 24-25 -6.4572114396608455 2215 26-27 -4.817674764065941 2215 28-29 -2.8869380725666574 2215 30-31 -4.9726842439831636 2215 32-33 -4.953616910701118 2215 34-35 -4.052393967649195 2215 36-37 -3.0726003318194586 2215 38-39 -2.8744216946485324 2215 40-41 -3.2160320165567455 2215 42-43 -3.000223139657507 2215 44-45 -4.4443375106268945 2215 46-47 -3.8572294536016685 2215 48-49 -3.0179150056879003 2215 50-51 -6.1440200354114545 2215 52-53 -6.635676754338682 2215 54-55 -4.255037620766153 2215 56-57 -3.1468984388534658 2215 58-59 -2.702275565697054 2215 60-61 -3.935068199202078 2215 62-63 -3.6172500421937457 2215 64-65 -2.788329700424697 2215 66-67 -2.5120938049007755 2215 68-69 -2.6637180869247032 2215 70-71 -2.258032346783427 2215 72-73 -2.2079056963091297 2215 74-75 -2.3717620385468194 2215 76-77 -2.5580126914825065 2215 78-79 -2.5156451450991533 2215 80-81 -2.525743240257089 2215 82-83 -2.4637892382680135 2215 84-85 -2.192681167370889 2215 86-87 -2.159789527900756 2215 88-89 -2.174534460364601 2215 90-91 -2.4357446786528136 2215 92-93 -4.542531114905582 2215 94-95 -2.982925109424169 2215 96-97 -2.5959704238448644 2215 98-99 -3.930715463131275 2215 100 -4.152585324051163 2216 1 -1.9390417338686952 2216 2 -2.004098185263956 2216 3 -2.145379678274214 2216 4 -2.257795760870742 2216 5 -2.565034652544991 2216 6 -2.241457616940288 2216 7 -2.6401702603447994 2216 8 -2.1398132882285843 2216 9 -2.54198112509885 2216 10-11 -2.56890806769691 2216 12-13 -2.8441648529521544 2216 14-15 -3.2161425480737904 2216 16-17 -2.9932472123529976 2216 18-19 -4.644049600012156 2216 20-21 -3.2983973395613617 2216 22-23 -2.725760165470504 2216 24-25 -2.9014215363585834 2216 26-27 -2.769124761951687 2216 28-29 -2.9435973109687623 2216 30-31 -2.9697674976447885 2216 32-33 -2.877460688701561 2216 34-35 -3.1187636672262187 2216 36-37 -3.180271697587415 2216 38-39 -3.378751203417721 2216 40-41 -3.397208050523389 2216 42-43 -3.177252962429222 2216 44-45 -3.2508241713716615 2216 46-47 -3.404050818020803 2216 48-49 -3.5434764153543767 2216 50-51 -3.2545538233633984 2216 52-53 -3.0414679321405984 2216 54-55 -3.0371938973128145 2216 56-57 -2.97858719697134 2216 58-59 -3.069035172990638 2216 60-61 -3.239520565279104 2216 62-63 -3.0504267619378105 2216 64-65 -2.9895844174668227 2216 66-67 -2.9113785690711502 2216 68-69 -3.1555130216394787 2216 70-71 -3.223470108432071 2216 72-73 -3.0344016672181375 2216 74-75 -3.115847226846899 2216 76-77 -2.8666244967975203 2216 78-79 -3.144104194756409 2216 80-81 -2.8904742751262837 2216 82-83 -3.040320378679162 2216 84-85 -2.845668613140557 2216 86-87 -2.8751809728199653 2216 88-89 -2.9106567011564515 2216 90-91 -2.8681145620847737 2216 92-93 -2.9613218919275184 2216 94-95 -2.586425925884253 2216 96-97 -2.7628908261765375 2216 98-99 -2.5659899275608815 2216 100 -2.754301283633101 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 15549.0 1 2233.0 2 423.0 3 411.0 4 322.0 5 212.0 6 192.0 7 273.0 8 544.0 9 945.0 10 1834.0 11 4119.0 12 7886.0 13 11818.0 14 16142.0 15 19596.0 16 22788.0 17 26334.0 18 29064.0 19 32678.0 20 36031.0 21 40242.0 22 45548.0 23 51979.0 24 58018.0 25 63875.0 26 68673.0 27 75849.0 28 85513.0 29 98826.0 30 114994.0 31 133994.0 32 160953.0 33 199541.0 34 269307.0 35 348228.0 36 501616.0 37 663818.0 38 454598.0 39 129599.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.753700171472175 22.030195389918884 12.658521272742417 23.557583165866525 2 15.343109358249846 19.773713257189744 41.11526092192856 23.76791646263185 3 18.254718879542803 27.6157981584137 28.800495876871718 25.32898708517178 4 12.053800243141009 17.08280031515141 40.691781706815995 30.17161773489158 5 12.921312035959302 39.123365023207654 34.938734850445705 13.016588090387335 6 28.793997379135877 39.356492861203556 18.46305834030827 13.386451419352294 7 26.463848218530146 31.97482491184349 24.37343665377368 17.18789021585268 8 26.140072004826454 35.16477579400497 19.875376669355678 18.8197755318129 9 26.10147978215547 15.168197516894447 22.047130427273952 36.68319227367613 10-11 25.764188148689353 26.169518411856664 27.134888178862955 20.931405260591028 12-13 25.794625593162824 24.089172376103047 27.261754047997677 22.854447982736456 14-15 23.427021676006007 25.04865919174947 24.71531972151909 26.80899941072543 16-17 22.134635721869827 27.493451322557334 26.23275144060307 24.139161514969768 18-19 21.441740337276578 28.4823693204599 27.927857881674644 22.14803246058888 20-21 23.362815330991992 27.491928599480385 26.62817119117646 22.517084878351163 22-23 24.182078659421443 27.00839185653606 26.304021417903336 22.505508066139164 24-25 23.38993238286206 27.1625504022578 27.185172891846882 22.262344323033258 26-27 23.18574308822507 27.385546436599718 26.05179817054397 23.376912304631244 28-29 22.095343435519272 27.914794242222822 26.892471353942156 23.09739096831575 30-31 23.211665607840008 27.403043196287125 26.686457918228918 22.698833277643953 32-33 23.658374233071957 27.688357103257527 25.59962959127103 23.05363907239949 34-35 23.555242485501893 27.449631146314125 26.79073583442633 22.204390533757657 36-37 23.14996877433502 27.046248164150814 26.325116801474458 23.478666260039706 38-39 23.43544824434458 27.021217550262115 27.07424321200661 22.469090993386693 40-41 22.796014153411644 25.965469596792033 27.980025726249913 23.258490523546406 42-43 22.899897059199503 26.297751529706126 27.051189170908618 23.751162240185753 44-45 23.04178778848322 27.462399575209197 26.79487495406181 22.700937682245776 46-47 23.036956136397418 27.073094346131622 26.28085540238309 23.609094115087874 48-49 23.904379937332973 26.512857230269937 26.65608177175671 22.926681060640377 50-51 23.410701171922604 26.434754780043303 26.494892774221306 23.65965127381279 52-53 22.62125357056669 27.167287291179964 26.395663425396975 23.81579571285637 54-55 22.73157242241886 27.052026046338128 26.288497489985975 23.927904041257033 56-57 24.001791769568474 26.36417795948598 26.031174777267204 23.602855493678344 58-59 22.64801725738818 26.797072835004972 27.19417502219295 23.3607348854139 60-61 22.680741853207877 27.63914052215601 26.231268672718933 23.44884895191718 62-63 23.280402398188947 26.71908770389791 25.707291315008174 24.293218582904963 64-65 22.21155652148804 26.930839899428342 26.391569295603684 24.46603428347993 66-67 23.21100798345295 26.374441612606102 26.32935471967317 24.085195684267774 68-69 22.390551916316852 26.579558103420087 26.66340499732257 24.366484982940488 70-71 22.34459318051166 26.194801272660325 26.90995742786978 24.55064811895824 72-73 23.125471158134676 26.353156890310608 27.27765011921104 23.243721832343674 74-75 23.34628401939384 26.71874757845219 26.73040412313179 23.20456427902218 76-77 22.85554746316938 26.674796137428586 26.34928619458785 24.120370204814186 78-79 22.729600833483705 26.580024742722586 26.4762499804586 24.214124443335113 80-81 22.224086836947347 26.982081773613288 27.34314300223339 23.450688387205982 82-83 23.257117509957276 25.754015857203843 28.49605305001704 22.492813582821842 84-85 23.548447345658488 26.413138649390078 26.75633466810962 23.282079336841814 86-87 21.868893016680353 27.286752181041745 27.384796086215925 23.45955871606198 88-89 23.193771627219544 25.65845199799738 27.21794322161274 23.92983315317034 90-91 22.78830664310266 25.765329604156374 27.44341927304761 24.00294447969336 92-93 23.042331196403296 26.132125551577722 27.271361252185493 23.55418199983349 94-95 21.625140326493973 26.06187540805752 28.165237502299647 24.147746763148863 96-97 22.105921624109723 25.998820579882004 27.488148186531674 24.407109609476603 98-99 21.29704765492339 26.89249576550459 27.445484368198365 24.36497221137365 100 22.30698574953341 25.990811547209496 26.420664481882827 25.281538221374262 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13119.0 1 7817.0 2 2277.0 3 1587.0 4 831.0 5 448.5 6 340.0 7 315.0 8 325.0 9 310.5 10 301.0 11 278.0 12 253.5 13 263.0 14 285.5 15 363.5 16 394.5 17 410.0 18 531.5 19 659.0 20 793.0 21 1046.5 22 1462.0 23 2031.0 24 2688.0 25 3516.0 26 4969.0 27 6777.5 28 9882.0 29 17123.0 30 27874.0 31 38254.0 32 52321.5 33 66498.0 34 77889.5 35 90024.5 36 101116.0 37 121588.0 38 129206.5 39 116800.0 40 104719.0 41 93576.5 42 90291.5 43 96484.5 44 107335.0 45 131016.5 46 155600.5 47 173217.0 48 185848.0 49 182641.5 50 173007.0 51 177124.5 52 178708.5 53 170735.0 54 162034.5 55 139455.0 56 121105.5 57 99218.5 58 76632.0 59 73120.5 60 61082.5 61 41963.0 62 31251.0 63 26215.5 64 19298.0 65 9289.5 66 4763.0 67 3096.5 68 2260.5 69 1666.5 70 1229.0 71 926.5 72 747.0 73 631.0 74 471.5 75 355.5 76 275.0 77 209.5 78 154.5 79 94.0 80 60.0 81 46.5 82 39.5 83 34.0 84 25.5 85 20.5 86 15.0 87 13.0 88 13.5 89 11.0 90 9.0 91 8.5 92 7.0 93 4.5 94 5.0 95 4.0 96 1.5 97 2.5 98 3.0 99 2.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.9785241733498621 2 0.4385715249491908 3 0.4703535979625741 4 0.4574931903087341 5 0.42423533281048403 6 0.4737004663478972 7 0.49114634722257366 8 0.493702616776718 9 0.5070110304348926 10-11 0.5209782764523827 12-13 0.5191862524350445 14-15 0.5168276325886946 16-17 0.5213735758679722 18-19 0.5201481476796451 20-21 0.5188831895497592 22-23 0.48258152655147113 24-25 0.5161951535237517 26-27 0.49753702110793463 28-29 0.5026232069218509 30-31 0.5056274824803298 32-33 0.5086976412747405 34-35 0.5215053423398351 36-37 0.5390039298032568 38-39 0.5271844772771355 40-41 0.5333116182187703 42-43 0.5295035671819263 44-45 0.5402161813443975 46-47 0.5380025046170972 48-49 0.5112802641232576 50-51 0.5254846897901013 52-53 0.5173415218289608 54-55 0.5273689503377439 56-57 0.5152859648678962 58-59 0.5203457973874398 60-61 0.5147457223332574 62-63 0.5093169436924971 64-65 0.521030983041128 66-67 0.5180794140713942 68-69 0.5389512232145116 70-71 0.5223750010541318 72-73 0.5266310580953104 74-75 0.5241274951299112 76-77 0.512268512662231 78-79 0.5416392592405191 80-81 0.5167617493527631 82-83 0.5162215068181243 84-85 0.5251420969632572 86-87 0.8296544134389152 88-89 1.0938988961132052 90-91 1.0838451143100498 92-93 1.0845566532581106 94-95 1.0881670545871598 96-97 1.081868617232103 98-99 1.0777970332515328 100 1.0738967456843844 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3794592.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.244944806913644 #Duplication Level Percentage of deduplicated Percentage of total 1 77.76832855651843 19.63257162135234 2 10.319950918441457 5.210531826922247 3 4.147061985383929 3.14077052795601 4 2.133243750453057 2.1541448295952352 5 1.2739992779294953 1.6081020727688973 6 0.7738477487493725 1.1721446223679373 7 0.5332800207081823 0.9423837282585479 8 0.38024173447380627 0.7679345280061082 9 0.2954186910278654 0.6712045694936223 >10 1.7189790464324852 8.421182745959747 >50 0.2415185179035825 4.338849666513284 >100 0.3090154294969399 17.278212195404034 >500 0.059348842116723456 10.561929283736593 >1k 0.04325778281042871 19.084697444602774 >5k 0.0017762857675779906 2.7152765330767896 >10k+ 7.314117866497608E-4 2.300063803985864 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 15838 0.41738347627360195 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 14100 0.37158145065398335 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 13128 0.34596604852379387 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 11413 0.30077014867474555 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 11365 0.29950519054485963 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 11022 0.29046601057505 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 10331 0.2722558841635675 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 8886 0.23417537379512737 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 7153 0.18850511464737182 No Hit GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT 6872 0.1810998389286648 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 6617 0.17437974886364593 No Hit TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC 6582 0.17345738356060414 No Hit GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT 6230 0.16418102394144088 No Hit GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT 6203 0.16346948499338004 No Hit GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT 6143 0.16188828733102267 No Hit CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG 5679 0.14966035874212563 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 5538 0.1459445442355858 No Hit GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA 5499 0.1449167657550535 No Hit TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC 5333 0.1405421188891981 No Hit ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA 5313 0.14001505300174563 No Hit ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG 5283 0.13922445417056697 No Hit GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT 5261 0.13864468169436925 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 5226 0.13772231639132745 No Hit TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG 5120 0.13492886718782943 No Hit CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC 4962 0.130765046676955 No Hit GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA 4950 0.1304488071444835 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 4774 0.1258106273349019 No Hit CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT 4771 0.12573156745178402 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 4761 0.12546803450805777 No Hit GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG 4617 0.12167316011840008 No Hit AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT 4608 0.12143598046904648 No Hit ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA 4577 0.12061902834349517 No Hit GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT 4480 0.11806275878935074 No Hit GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC 4409 0.11619167488889451 No Hit GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC 4221 0.1112372555468414 No Hit GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA 4187 0.11034124353817222 No Hit CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC 4151 0.10939252494075778 No Hit ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG 4100 0.10804850692775403 No Hit GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA 4039 0.10644095597102403 No Hit AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA 3919 0.10327856064630926 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 3889 0.10248796181513058 No Hit GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT 3835 0.10106488391900895 No Hit GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA 3817 0.10059052462030173 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 3.95299415589344E-5 0.0 22-23 0.0 0.0 0.0 9.223653030418026E-5 0.0 24-25 0.0 0.0 0.0 3.162395324714752E-4 0.0 26-27 0.0 0.0 0.0 5.92949123384016E-4 0.0 28-29 0.0 0.0 0.0 0.001673434192661556 0.0 30-31 0.0 0.0 0.0 0.005600075054182373 0.0 32-33 0.0 0.0 0.0 0.015508913738288596 0.0 34-35 0.0 0.0 0.0 0.030688411296919405 0.0 36-37 0.0 0.0 0.0 0.05858337339034078 0.0 38-39 0.0 0.0 0.0 0.10427998583246895 0.0 40-41 0.0 0.0 0.0 0.15822517941322808 0.0 42-43 0.0 0.0 0.0 0.21402828024725717 0.0 44-45 0.0 0.0 0.0 0.2827049653823125 0.0 46-47 0.0 0.0 0.0 0.37260922913451566 0.0 48-49 0.0 0.0 0.0 0.47995937376139514 0.0 50-51 0.0 0.0 0.0 0.5987600247931794 0.0 52-53 0.0 0.0 0.0 0.716269364400705 0.0 54-55 0.0 0.0 0.0 0.8471134709607778 0.0 56-57 0.0 0.0 0.0 1.0086986954065154 0.0 58-59 0.0 0.0 0.0 1.2092340889349895 0.0 60-61 0.0 0.0 0.0 1.4216021116367714 0.0 62-63 0.0 0.0 0.0 1.6485303294794276 0.0 64-65 0.0 0.0 0.0 1.8800835504844788 0.0 66-67 0.0 0.0 0.0 2.148267850667476 0.0 68-69 0.0 0.0 0.0 2.4536893557989896 0.0 70-71 0.0 0.0 0.0 2.7977974970695136 0.0 72-73 0.0 0.0 0.0 3.1642795852623946 0.0 74-75 0.0 0.0 0.0 3.5241997031564924 0.0 76-77 0.0 0.0 0.0 3.9367209966183454 0.0 78-79 0.0 0.0 0.0 4.383606985942098 0.0 80-81 0.0 0.0 0.0 4.865068497482733 0.0 82-83 0.0 0.0 0.0 5.374266851350553 0.0 84-85 0.0 0.0 0.0 5.862988695490846 0.0 86-87 0.0 0.0 0.0 6.391490837486613 0.0 88 0.0 0.0 0.0 6.817386427842572 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGCACC 4335 0.0 39.09994 94 GTACTAG 4740 0.0 37.32102 1 TATTCTC 1985 0.0 35.275265 5 CAACGCA 7655 0.0 32.720997 5 TAGGCAT 6145 0.0 31.660883 5 GTATCAA 8820 0.0 31.502665 1 GCATCGT 5530 0.0 30.394814 8 ATCAACG 8275 0.0 30.103415 3 AGGGCTA 5105 0.0 29.457695 5 GGTATCA 5895 0.0 28.968456 1 CTAGGCA 6780 0.0 28.90583 4 CTATTGA 5445 0.0 27.593035 9 TTCTCTG 2710 0.0 27.373077 7 CTGTGCG 6540 0.0 27.280556 9 AACGCAG 10040 0.0 27.166048 6 TAGTACT 6720 0.0 26.99586 4 GCTATTG 5570 0.0 26.720568 8 AAGCTAA 2730 0.0 26.657316 9 TATCAAC 10495 0.0 26.557775 2 GTACATG 17280 0.0 26.453346 1 >>END_MODULE