##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146475_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3794592
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.788926714650746	31.0	31.0	34.0	30.0	34.0
2	31.748158695322186	31.0	31.0	34.0	30.0	34.0
3	31.92710494303472	31.0	31.0	34.0	30.0	34.0
4	35.68431283257858	37.0	35.0	37.0	33.0	37.0
5	35.604629957581736	37.0	35.0	37.0	33.0	37.0
6	35.65093928411803	37.0	35.0	37.0	33.0	37.0
7	35.572214087838695	37.0	35.0	37.0	33.0	37.0
8	35.564239317428594	37.0	35.0	37.0	33.0	37.0
9	37.13844255192653	39.0	37.0	39.0	33.0	39.0
10-11	37.08275553735422	39.0	37.0	39.0	33.0	39.0
12-13	37.01330801835876	39.0	37.0	39.0	33.0	39.0
14-15	38.19133031957059	40.0	37.0	41.0	33.0	41.0
16-17	38.202041352535396	40.0	37.0	41.0	33.0	41.0
18-19	38.251979395940324	40.0	37.5	41.0	33.0	41.0
20-21	38.26360923651344	40.0	37.0	41.0	33.0	41.0
22-23	38.22656520121267	40.0	37.0	41.0	33.0	41.0
24-25	38.149617139339355	40.0	37.0	41.0	33.0	41.0
26-27	37.99619168543021	40.0	37.0	41.0	33.0	41.0
28-29	37.93205659528087	40.0	37.0	41.0	33.0	41.0
30-31	37.798245239540904	40.0	37.0	41.0	32.5	41.0
32-33	37.67206474371949	40.0	36.0	41.0	32.0	41.0
34-35	37.571828802674965	40.0	36.0	41.0	32.0	41.0
36-37	37.49235438223661	40.0	36.0	41.0	32.0	41.0
38-39	37.36104948832444	40.0	36.0	41.0	31.0	41.0
40-41	37.22658088142282	39.0	35.0	41.0	31.0	41.0
42-43	37.08845377842994	39.0	35.0	41.0	31.0	41.0
44-45	36.865104074430135	39.0	35.0	41.0	30.5	41.0
46-47	36.606309453032104	39.0	35.0	41.0	30.0	41.0
48-49	36.316211861512386	39.0	35.0	41.0	29.5	41.0
50-51	36.09010415349002	38.5	35.0	41.0	29.0	41.0
52-53	35.87918582551167	38.0	35.0	40.0	29.0	41.0
54-55	35.665040404870936	38.0	34.0	40.0	28.0	41.0
56-57	35.37753887637986	37.0	34.0	40.0	28.0	41.0
58-59	35.06834661012304	37.0	34.0	40.0	28.0	41.0
60-61	34.8012990592928	36.0	34.0	39.5	27.0	41.0
62-63	34.51753311027905	35.5	33.5	39.0	27.0	41.0
64-65	34.239981663377776	35.0	33.0	39.0	26.0	41.0
66-67	34.02439498106779	35.0	33.0	38.5	26.0	41.0
68-69	33.844223700466344	35.0	33.0	38.0	26.0	40.5
70-71	33.67116820464493	35.0	33.0	37.0	26.0	40.0
72-73	33.49665906110591	35.0	33.0	37.0	26.0	40.0
74-75	33.3359611520817	35.0	33.0	36.0	26.0	39.0
76-77	33.20348867546234	35.0	33.0	36.0	26.0	39.0
78-79	33.10853446167599	35.0	33.0	36.0	26.5	39.0
80-81	32.989520348959786	35.0	33.0	35.0	26.5	38.0
82-83	32.80771331937663	35.0	33.0	35.0	26.0	37.0
84-85	32.679289104072325	35.0	33.0	35.0	26.0	37.0
86-87	32.56244966520775	35.0	33.0	35.0	26.0	36.0
88-89	32.46404738638567	35.0	33.0	35.0	26.0	36.0
90-91	32.37861264135907	35.0	33.0	35.0	26.0	36.0
92-93	32.30034414767121	35.0	33.0	35.0	26.0	36.0
94-95	32.26674303851376	35.0	33.0	35.0	26.0	35.0
96-97	32.21819262782402	35.0	33.0	35.0	25.5	35.0
98-99	32.17918975215254	35.0	33.0	35.0	25.0	35.0
100	31.672741101019557	34.0	32.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.5302157836731034
1101	2	0.6236080045426107
1101	3	0.5501710709553436
1101	4	0.3692749620800839
1101	5	0.3565952498784526
1101	6	0.39656223501495447
1101	7	0.4204061731411741
1101	8	0.4198231775201151
1101	9	0.49467716856835864
1101	10-11	0.5438703678052832
1101	12-13	0.5284021274692492
1101	14-15	0.6551337217344368
1101	16-17	0.6841937266671252
1101	18-19	0.649965425507375
1101	20-21	0.667905446547806
1101	22-23	0.6224736305327028
1101	24-25	0.6844944685445427
1101	26-27	0.6901195421081994
1101	28-29	0.7077286616466054
1101	30-31	0.6869001331518447
1101	32-33	0.725561006115079
1101	34-35	0.7275412427107142
1101	36-37	0.6932108213075097
1101	38-39	0.775519393252381
1101	40-41	0.7408502261748495
1101	42-43	0.6884676439998003
1101	44-45	0.7135955812575006
1101	46-47	0.7776661759210342
1101	48-49	0.7022484494221288
1101	50-51	0.6522084719551344
1101	52-53	0.6809471096210586
1101	54-55	0.6959919284163263
1101	56-57	0.688851460667081
1101	58-59	0.6936812616702142
1101	60-61	0.6962374370278184
1101	62-63	0.629106272074381
1101	64-65	0.6336774905564582
1101	66-67	0.6108357929377064
1101	68-69	0.5720570521044621
1101	70-71	0.6299538139546854
1101	72-73	0.510340392412509
1101	74-75	0.534397408138652
1101	76-77	0.5202761040339539
1101	78-79	0.4325627533501972
1101	80-81	0.4835839654526737
1101	82-83	0.47509875937109314
1101	84-85	0.4958263009060957
1101	86-87	0.5045789818799236
1101	88-89	0.48761207590857225
1101	90-91	0.47964368646989897
1101	92-93	0.5492642424765677
1101	94-95	0.4383315834288197
1101	96-97	0.4740849099116531
1101	98-99	0.5253970643384918
1101	100	0.5406752841325968
1102	1	0.475921769756102
1102	2	0.5829859633400574
1102	3	0.47814739910580073
1102	4	0.3650859676521492
1102	5	0.37580463917885965
1102	6	0.38368300902407526
1102	7	0.41213767198777873
1102	8	0.40559246270176885
1102	9	0.4948667698420195
1102	10-11	0.545232513942679
1102	12-13	0.5620060196151471
1102	14-15	0.6851819500536109
1102	16-17	0.6745553873583461
1102	18-19	0.66380526669343
1102	20-21	0.6973937669217989
1102	22-23	0.6378445254471643
1102	24-25	0.7034161611656202
1102	26-27	0.7727019469376586
1102	28-29	0.7664980487633315
1102	30-31	0.7692493823982076
1102	32-33	0.8023252547103539
1102	34-35	0.8127896857677044
1102	36-37	0.7737870413996859
1102	38-39	0.8060875487642534
1102	40-41	0.7845193709638707
1102	42-43	0.8153585688530711
1102	44-45	0.7985846753192405
1102	46-47	0.7889630765117204
1102	48-49	0.7786344078675995
1102	50-51	0.8485553032467053
1102	52-53	0.7288909128274597
1102	54-55	0.8024414738725554
1102	56-57	0.7437071059467968
1102	58-59	0.7042871478608888
1102	60-61	0.7607341081201184
1102	62-63	0.7501876577811615
1102	64-65	0.7419040315616527
1102	66-67	0.7697160464909416
1102	68-69	0.6324452351778405
1102	70-71	0.6362337193674534
1102	72-73	0.6858931646487392
1102	74-75	0.6777019715506114
1102	76-77	0.6383825922802799
1102	78-79	0.5529901781340385
1102	80-81	0.5291855301738693
1102	82-83	0.6043427574301177
1102	84-85	0.6424737114230936
1102	86-87	0.6487318088701457
1102	88-89	0.60114047020096
1102	90-91	0.6225375569478757
1102	92-93	0.5916942880716078
1102	94-95	0.6035800041827528
1102	96-97	0.5512592811924577
1102	98-99	0.5800971585131194
1102	100	0.6221640157951143
1103	1	0.3342524381283418
1103	2	-0.1344748617318956
1103	3	0.22279082731174782
1103	4	0.213343083098259
1103	5	0.2761883935760423
1103	6	0.2690363828648188
1103	7	0.3334680205646876
1103	8	0.3384354431942569
1103	9	0.38677594796801884
1103	10-11	0.40628144262831256
1103	12-13	0.41122252105422774
1103	14-15	0.5052970763727629
1103	16-17	0.5735800115988425
1103	18-19	0.5422830409179227
1103	20-21	0.5722738436726758
1103	22-23	0.5103261372156354
1103	24-25	0.5672632989974176
1103	26-27	0.6271151225052947
1103	28-29	0.5519953305030256
1103	30-31	0.6318972877387949
1103	32-33	0.6330735757848629
1103	34-35	0.6190736640216272
1103	36-37	0.6289197564620466
1103	38-39	0.611252243359715
1103	40-41	0.6109504550700393
1103	42-43	0.6034941620734102
1103	44-45	0.614702360001047
1103	46-47	0.7030980301414047
1103	48-49	0.646239140838496
1103	50-51	0.5747305510880878
1103	52-53	0.6032912965818582
1103	54-55	0.6502188963221869
1103	56-57	0.6254627823139174
1103	58-59	0.5435100452065882
1103	60-61	0.598437977643627
1103	62-63	0.6361006734511321
1103	64-65	0.6469248182581637
1103	66-67	0.72166679800997
1103	68-69	0.6445974577553883
1103	70-71	0.6099305533936814
1103	72-73	0.5056085468041616
1103	74-75	0.5245999568400208
1103	76-77	0.5193387803199272
1103	78-79	0.5222898263591773
1103	80-81	0.4601097386804227
1103	82-83	0.48369996881483246
1103	84-85	0.4939287801790897
1103	86-87	0.46540913538117934
1103	88-89	0.44995722747630396
1103	90-91	0.4670370772548793
1103	92-93	0.4752887875919214
1103	94-95	0.5181946508089155
1103	96-97	0.5113648136516886
1103	98-99	0.5877722464568365
1103	100	0.6090524072246026
1104	1	0.41878383337430236
1104	2	0.49017030768863634
1104	3	0.440585242014798
1104	4	0.30112965526167557
1104	5	0.2824554018091163
1104	6	0.31832478140872666
1104	7	0.31059196760609353
1104	8	0.31887366031785547
1104	9	0.36832167559284557
1104	10-11	0.37268038449938956
1104	12-13	0.41413540479409505
1104	14-15	0.5035129503985658
1104	16-17	0.48269862757275206
1104	18-19	0.5320059968009758
1104	20-21	0.5405166615541077
1104	22-23	0.5200875246251115
1104	24-25	0.5214808506728588
1104	26-27	0.6205999937203472
1104	28-29	0.5766986987325637
1104	30-31	0.5612871708980407
1104	32-33	0.5626768158385858
1104	34-35	0.5745287741099929
1104	36-37	0.6185301346871057
1104	38-39	0.58059856735305
1104	40-41	0.5917971325360085
1104	42-43	0.5908965301353533
1104	44-45	0.6039403552840028
1104	46-47	0.6132555514481624
1104	48-49	0.6163254810302874
1104	50-51	0.5981311032542109
1104	52-53	0.5745198098751416
1104	54-55	0.5856708690915369
1104	56-57	0.5978164153157053
1104	58-59	0.6050299330250724
1104	60-61	0.5181186946723528
1104	62-63	0.5371992934501506
1104	64-65	0.5314080648280495
1104	66-67	0.6342267002634614
1104	68-69	0.5602690231502478
1104	70-71	0.48507097861080695
1104	72-73	0.5212407479151508
1104	74-75	0.5620133325513379
1104	76-77	0.4834678776881347
1104	78-79	0.43587919773473516
1104	80-81	0.432035268357545
1104	82-83	0.38440989777519974
1104	84-85	0.35287636451670323
1104	86-87	0.3566577661200441
1104	88-89	0.4294163777282165
1104	90-91	0.4145661492476336
1104	92-93	0.4447822760506597
1104	94-95	0.3907101798812178
1104	96-97	0.3736583873824486
1104	98-99	0.39758170422311423
1104	100	0.4784003001300974
1105	1	0.24148678849928373
1105	2	-0.36014547575752687
1105	3	0.11890664840548482
1105	4	0.17411741901785405
1105	5	0.20757372453456213
1105	6	0.26275034149627885
1105	7	0.22563356769639853
1105	8	0.30650310290005933
1105	9	0.3661842296066382
1105	10-11	0.3718688289515768
1105	12-13	0.367601555869399
1105	14-15	0.42817454731689963
1105	16-17	0.44485556827415706
1105	18-19	0.4982718681345162
1105	20-21	0.4188000285676523
1105	22-23	0.4188975667922321
1105	24-25	0.46139599199625536
1105	26-27	0.492905822366545
1105	28-29	0.5233063270145237
1105	30-31	0.5213945567127993
1105	32-33	0.5245922950193886
1105	34-35	0.5080028225960262
1105	36-37	0.5410316641213626
1105	38-39	0.5188612839933171
1105	40-41	0.5690422770897428
1105	42-43	0.5612675080497951
1105	44-45	0.5565122246642531
1105	46-47	0.49260440094386126
1105	48-49	0.5194294236842225
1105	50-51	0.5389300970420408
1105	52-53	0.529939467589287
1105	54-55	0.5544000129582258
1105	56-57	0.5466883040620374
1105	58-59	0.5197616483924037
1105	60-61	0.4882464728610927
1105	62-63	0.5941095688173448
1105	64-65	0.5242855955894967
1105	66-67	0.5301994329903152
1105	68-69	0.48281654052528467
1105	70-71	0.5301500491885633
1105	72-73	0.4840192459306323
1105	74-75	0.4540201020159387
1105	76-77	0.4710870400287206
1105	78-79	0.3743075901530091
1105	80-81	0.39529548508640033
1105	82-83	0.43540545847385204
1105	84-85	0.4408174374764542
1105	86-87	0.43636868622228064
1105	88-89	0.42300174285570336
1105	90-91	0.4494119594047987
1105	92-93	0.4038031098542376
1105	94-95	0.40519959690634266
1105	96-97	0.426305789223953
1105	98-99	0.38231247872484175
1105	100	0.5152983221445666
1106	1	0.2773003354518231
1106	2	0.2526363003691898
1106	3	0.21790716063859605
1106	4	0.18396091207795706
1106	5	0.21796407023344244
1106	6	0.16741852851080807
1106	7	0.24077214987376294
1106	8	0.2573061618589776
1106	9	0.22995069711035399
1106	10-11	0.26587579128500494
1106	12-13	0.26660604194849213
1106	14-15	0.3828449856922802
1106	16-17	0.32537650309158295
1106	18-19	0.3336677803716128
1106	20-21	0.3427729070445764
1106	22-23	0.3600486439252606
1106	24-25	0.27348004519494395
1106	26-27	0.3511555217686322
1106	28-29	0.34011690367488967
1106	30-31	0.37906785436497614
1106	32-33	0.3526018149763672
1106	34-35	0.3554642943639905
1106	36-37	0.3840564751368376
1106	38-39	0.4019858157381577
1106	40-41	0.38211421190197115
1106	42-43	0.38265828184688644
1106	44-45	0.4198217362708121
1106	46-47	0.41556932236433397
1106	48-49	0.40772627502506964
1106	50-51	0.48263292672606184
1106	52-53	0.4327320259877112
1106	54-55	0.45026010242943926
1106	56-57	0.4663856105368325
1106	58-59	0.4269010071922139
1106	60-61	0.4284465683789591
1106	62-63	0.3295593730047557
1106	64-65	0.3841870611914473
1106	66-67	0.44379301635959223
1106	68-69	0.3684719366563485
1106	70-71	0.38956846688266467
1106	72-73	0.3618495861913118
1106	74-75	0.3089691923909541
1106	76-77	0.3219115184541579
1106	78-79	0.3449027674760572
1106	80-81	0.35704384534120237
1106	82-83	0.3239302115798708
1106	84-85	0.33986076438224444
1106	86-87	0.33838046151880263
1106	88-89	0.38695078407923944
1106	90-91	0.36237756351162886
1106	92-93	0.35827538965841654
1106	94-95	0.3426276558591965
1106	96-97	0.31794637237106116
1106	98-99	0.33562061331372206
1106	100	0.40999926403340226
1107	1	0.1945386743703459
1107	2	-0.3719009624687182
1107	3	0.04626991567495509
1107	4	0.10319464368652831
1107	5	0.15370429594913304
1107	6	0.228320041323407
1107	7	0.20492350363042533
1107	8	0.21135250744722356
1107	9	0.19288222618477846
1107	10-11	0.3223790980236032
1107	12-13	0.22556008509421588
1107	14-15	0.3182224138733645
1107	16-17	0.38556354537608684
1107	18-19	0.38907263242147394
1107	20-21	0.3431333421766283
1107	22-23	0.29443495049011403
1107	24-25	0.2984099918346459
1107	26-27	0.3930066329102928
1107	28-29	0.29916734284427093
1107	30-31	0.38570656801803693
1107	32-33	0.34990757611372203
1107	34-35	0.37702895482174625
1107	36-37	0.3592727224666419
1107	38-39	0.437662094091678
1107	40-41	0.397310485055705
1107	42-43	0.3646595117961482
1107	44-45	0.3930373786255643
1107	46-47	0.37168519508458076
1107	48-49	0.4332611342121311
1107	50-51	0.3605992356158225
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2216	100	-1.1020334754535348
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	8.0
13	45.0
14	244.0
15	725.0
16	1754.0
17	3640.0
18	6520.0
19	9933.0
20	14505.0
21	20584.0
22	28409.0
23	32892.0
24	33818.0
25	35379.0
26	38816.0
27	48047.0
28	60273.0
29	75737.0
30	93548.0
31	115163.0
32	143152.0
33	180478.0
34	250120.0
35	345914.0
36	515738.0
37	854539.0
38	634882.0
39	249327.0
40	399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.38073133343314	22.727603388036442	13.975966617256045	23.915698661274373
2	14.899560174686208	20.533667364086757	40.283033611361404	24.28373884986563
3	17.988827610670405	27.2826194949418	29.832551199317543	24.89600169507025
4	12.400959915817577	17.739542700912523	39.35064394177031	30.508853441499596
5	12.484569739506632	39.140891587068836	35.1013785244507	13.273160148973828
6	28.706669913392535	39.73238756630489	17.85841534478542	13.702527175517156
7	25.46946528775362	32.59712595560566	24.5145777546571	17.418831001983616
8	26.299375532336544	34.85623750853847	19.644457164301198	19.19992979482379
9	26.05637180492659	14.449195064976683	22.148257309349727	37.346175820747
10-11	25.79186378930857	26.09098685708503	27.00175671060288	21.115392643003517
12-13	26.090644264258184	23.889340408665806	27.722440251810998	22.29757507526501
14-15	23.502628128772578	24.65894680607273	25.603114932518096	26.23531013263659
16-17	21.578309185606184	27.098944232572958	27.132598285250907	24.190148296569948
18-19	20.913803117700137	27.257436372606065	29.92587872424756	21.902881785446233
20-21	22.69182512256268	26.43063583434201	28.945624941199167	21.931914101896147
22-23	23.237301520058264	25.91297511950336	28.738152277794637	22.11157108264374
24-25	22.66733814860728	26.088325174353393	29.52848422175559	21.71585245528373
26-27	22.50469518180068	26.338938826323798	28.37540846072222	22.780957531153305
28-29	21.41094483939248	26.977669799546298	29.157561603460923	22.45382375760029
30-31	22.595617657972188	26.38506326898913	28.852377277978764	22.166941795059916
32-33	23.162516549868865	26.735061371551932	27.867119837916697	22.2353022406625
34-35	22.964455642321386	26.52247548041365	28.839629591612326	21.673439285652638
36-37	22.65591399549675	26.071432712660542	28.438815029389193	22.83383826245351
38-39	22.878230386824196	25.913141650011383	29.3095805820494	21.899047381115018
40-41	22.23636084289897	25.13433007314924	29.96573262168503	22.66357646226675
42-43	22.54081306460807	25.391606978100473	28.848176480153022	23.219403477138435
44-45	22.736924022398192	26.49384966815932	28.423200702473416	22.346025606969075
46-47	22.79214735075602	26.283207786238943	27.58080447120534	23.3438403917997
48-49	23.900381203115508	25.941432021852307	27.58980278371829	22.568383991313894
50-51	23.181635864936204	26.002281668226786	27.25491963299348	23.561162833843532
52-53	22.299011329808316	26.653972284767374	27.20980279302755	23.837213592396758
54-55	22.542794000064305	26.630380643518876	26.925090932473577	23.901734423943243
56-57	23.78289190212695	25.95120314742914	26.691942131282204	23.573962819161707
58-59	22.3567165258805	26.43421170354752	27.856844405494197	23.352227365077784
60-61	22.54681174172423	27.190636663405442	26.85374892527844	23.408802669591886
62-63	23.1642163373559	26.22065824204552	26.2954752447694	24.31965017582918
64-65	22.039642206592962	26.471633841003197	26.914092476872348	24.574631475531493
66-67	22.933506421770772	25.98693087425473	26.901416542279115	24.178146161695384
68-69	22.276469858082667	25.85658103381892	27.39924781257667	24.467701295521742
70-71	22.222002609265463	25.536396960433695	27.595782324380895	24.645818105919943
72-73	22.978148902437994	25.633928496133446	27.877700685607305	23.51022191582125
74-75	23.101807734391965	26.1562795816353	27.30278207044567	23.439130613527066
76-77	22.74201847821862	26.15801821994786	26.683296141026375	24.416667160807147
78-79	22.44573596781613	26.175577400504906	26.98802750191952	24.39065912975945
80-81	21.95207152770128	26.718920232360666	27.72171484322086	23.607293396717203
82-83	23.04308994425657	25.422819386231964	28.861876892198453	22.672213777313015
84-85	23.443868605838205	26.064277609231258	27.007846758203176	23.484007026727365
86-87	21.655382584799288	26.883893478688858	27.63498400092982	23.825739935582032
88-89	22.771152162952614	25.443318510147243	27.476338731896767	24.309190595003376
90-91	22.39281092235047	25.417285850146992	27.746654432563062	24.443248794939475
92-93	22.668461498420957	25.948364983385947	27.30649387787106	24.07667964032204
94-95	21.321136845489633	25.732336109694597	28.188579101260103	24.757947943555667
96-97	21.79784513907081	25.61551265839799	27.559589162760467	25.027053039770735
98-99	20.96360182084452	26.54418773683932	27.553476700374198	24.938733741941963
100	22.00456341476988	25.65497872982406	26.567213307594777	25.77324454781128
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	218.0
1	141.5
2	108.0
3	143.0
4	89.5
5	63.5
6	150.5
7	403.5
8	674.5
9	782.0
10	659.5
11	467.5
12	427.5
13	463.0
14	590.5
15	902.5
16	1256.5
17	1691.5
18	2331.5
19	3074.5
20	3919.0
21	4774.0
22	5583.0
23	6320.0
24	7104.5
25	7933.0
26	9215.5
27	10606.0
28	13098.5
29	19885.0
30	30037.5
31	39342.0
32	53277.5
33	66914.5
34	76485.0
35	88087.0
36	98999.0
37	120646.5
38	128767.5
39	114736.0
40	101974.0
41	90244.0
42	86705.5
43	92984.0
44	104008.5
45	130470.0
46	156641.0
47	174743.5
48	188081.5
49	182949.0
50	172640.0
51	175959.5
52	176860.5
53	170211.0
54	164458.5
55	141217.5
56	121392.0
57	99217.5
58	75776.0
59	74494.0
60	62039.5
61	40821.5
62	29259.5
63	24322.0
64	17435.0
65	7139.0
66	3290.0
67	2235.0
68	1653.5
69	1206.5
70	912.5
71	713.5
72	587.0
73	544.0
74	400.5
75	266.0
76	184.0
77	124.0
78	80.5
79	40.0
80	21.5
81	11.0
82	7.0
83	4.0
84	1.5
85	1.5
86	2.5
87	1.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01412536578372589
2	0.003452281562813604
3	0.0015548443679847532
4	0.024403150589048836
5	0.0026353294372622933
6	0.0
7	2.371796493536064E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.009289536266349584
16-17	0.002661682731634916
18-19	0.0
20-21	7.90598831178688E-5
22-23	8.828353614828682E-4
24-25	0.0
26-27	2.767095909125408E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	6.193024177566389E-4
36-37	0.0
38-39	0.0
40-41	5.534191818250816E-4
42-43	9.223653030418027E-4
44-45	0.0
46-47	0.0
48-49	7.115389480608193E-4
50-51	0.0
52-53	0.0
54-55	4.743592987072128E-4
56-57	7.90598831178688E-5
58-59	1.9764970779467198E-4
60-61	1.185898246768032E-4
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0015548443679847532
70-71	9.8824853897336E-4
72-73	0.0
74-75	2.1082635498098347E-4
76-77	0.003280985149391555
78-79	5.402425346387701E-4
80-81	0.0038475809784029483
82-83	0.0016866108398478677
84-85	0.005863607997908602
86-87	0.008143167961140486
88-89	0.008195874549885733
90-91	0.007392099071520732
92-93	0.009605775798821058
94-95	0.0015021377792395073
96-97	0.0051125391082888485
98-99	0.002345443199163441
100	0.08256487126942766
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3794592.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.86867526236425
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.07444976341725	10.64382661697433
2	14.71291083671185	4.669488084638005
3	6.159483631811362	2.9322853661118744
4	3.203634261709555	2.0334972703381196
5	1.887479514528252	1.497589974020688
6	1.235456579268508	1.1763035554297998
7	0.8395489817668161	0.9325771110954321
8	0.5939894426368751	0.7540660459581847
9	0.43815024590301516	0.6257577571523991
>10	2.699159833339071	8.464145982220964
>50	0.41851343884445663	4.743855090022177
>100	0.5412514791814567	19.287559258427017
>500	0.10712028315140779	12.060841109213507
>1k	0.08370329102063492	23.6874412326838
>5k	0.004151948959356892	4.249853303330672
>10k+	9.96467750245654E-4	2.240912242383057
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	18157	0.4784967659237146	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	14991	0.3950622359399904	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	14245	0.3754026783380137	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	12731	0.33550379065786257	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	12559	0.33097102402577144	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	12347	0.3253841256187754	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	9921	0.2614510334707921	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7938	0.20919245072988082	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	7771	0.2047914505696528	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	7742	0.20402720503284677	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	7414	0.1953833244786264	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7334	0.1932750609288166	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	6869	0.18102077904554692	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	6668	0.1757237668766497	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	6439	0.16968886246531906	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	6403	0.16874014386790465	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6366	0.1677650719761176	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	6164	0.16244170651284776	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	6157	0.1622572334522394	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	6107	0.16093956873360826	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	6061	0.1597273171924676	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	5886	0.15511549067725858	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	5881	0.15498372420539547	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5830	0.1536397061923917	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	5605	0.14771021495855152	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	5577	0.1469723227161181	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	5553	0.14633984365117514	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	5463	0.14396804715763908	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	5440	0.14336192138706877	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	5369	0.1414908374866125	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	5300	0.13967246017490154	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	4949	0.1304224538501109	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	4936	0.1300798610232668	No Hit
AATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGA	4754	0.12528356144744943	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	4716	0.12428213626128974	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	4667	0.12299082483703122	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	4664	0.12291176495391337	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	4654	0.12264823201018712	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	4646	0.12243740565520615	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	4631	0.12204210623961681	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	4626	0.1219103397677537	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	4539	0.1196176031573355	No Hit
ATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAATTT	4463	0.11761475278501615	No Hit
ATCCTATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTC	4380	0.11542742935208845	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4342	0.11442600416592878	No Hit
TAGTAGCCTAGAGCGCCAGGGGCGGAAATTCGCCTGAAAAGTTTTGCCGG	4236	0.11163255496243074	No Hit
ATATAGATTGGCGCTAGTAGATCACAGAACAACCGCCGCATACGGCCGAT	4205	0.11081560283687944	No Hit
GCCTTATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGA	4170	0.10989323753383762	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	4121	0.1086019261095791	No Hit
GGTGTACACCGCCCTTAGACGTCTTGGTATACGGACAACTGATGGACCCA	4070	0.10725790809657533	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	4031	0.10623012961604304	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	4007	0.10559765055110008	No Hit
GGCTTATATTGAAGTATAAACCAATGAGAGAGCCTCACTTAGTTACAGTT	3978	0.10483340501429403	No Hit
ATCTATCCCTACCAAGGCTTATATTGAAGTATAAACCAATGAGAGAGCCT	3887	0.10243525522638534	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	3.95299415589344E-5	0.0
22-23	0.0	0.0	0.0	1.0541317749049173E-4	0.0
24-25	0.0	0.0	0.0	3.4259282684409814E-4	0.0
26-27	0.0	0.0	0.0	6.193024177566389E-4	0.0
28-29	0.0	0.0	0.0	0.001778847370152048	0.0
30-31	0.0	0.0	0.0	0.005969021175399094	0.0
32-33	0.0	0.0	0.0	0.01625998262790835	0.0
34-35	0.0	0.0	0.0	0.031887486190873754	0.0
36-37	0.0	0.0	0.0	0.061021053119808405	0.0
38-39	0.0	0.0	0.0	0.10790356380870461	0.0
40-41	0.0	0.0	0.0	0.16395702093927356	0.0
42-43	0.0	0.0	0.0	0.22207921167809347	0.0
44-45	0.0	0.0	0.0	0.2933385196616659	0.0
46-47	0.0	0.0	0.0	0.3861943523836028	0.0
48-49	0.0	0.0	0.0	0.49761608099105253	0.0
50-51	0.0	0.0	0.0	0.6207782022415058	0.0
52-53	0.0	0.0	0.0	0.7424908923014648	0.0
54-55	0.0	0.0	0.0	0.8770112834265186	0.0
56-57	0.0	0.0	0.0	1.044301996103929	0.0
58-59	0.0	0.0	0.0	1.2510435904571557	0.0
60-61	0.0	0.0	0.0	1.469934053516162	0.0
62-63	0.0	0.0	0.0	1.7034110650104148	0.0
64-65	0.0	0.0	0.0	1.941710729374858	0.0
66-67	0.0	0.0	0.0	2.21798549093025	0.0
68-69	0.0	0.0	0.0	2.530996744841079	0.0
70-71	0.0	0.0	0.0	2.8851850212091312	0.0
72-73	0.0	0.0	0.0	3.2620371307376392	0.0
74-75	0.0	0.0	0.0	3.6323009166729916	0.0
76-77	0.0	0.0	0.0	4.059659114866632	0.0
78-79	0.0	0.0	0.0	4.522910499995783	0.0
80-81	0.0	0.0	0.0	5.021672949292045	0.0
82-83	0.0	0.0	0.0	5.549727085283477	0.0
84-85	0.0	0.0	0.0	6.056817175601488	0.0
86-87	0.0	0.0	0.0	6.604596752430828	0.0
88	0.0	0.0	0.0	7.0455796038151135	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	3995	0.0	41.681202	94
GGTATCA	8385	0.0	41.606377	1
GTGGTAT	1870	0.0	38.217392	1
TATTCTC	2280	0.0	37.927406	5
GTATCAA	14030	0.0	36.796265	1
GTACTAG	5030	0.0	32.902866	1
TACCCCG	295	0.0	31.862373	5
TAGGCAT	5920	0.0	31.834118	5
CAACGCA	15900	0.0	30.799295	5
ATCAACG	16405	0.0	29.915186	3
TATCCGC	175	1.70985E-10	29.553825	1
TTCTCTG	2995	0.0	29.499846	7
CTAGGCA	6745	0.0	28.8519	4
TATCAAC	18095	0.0	28.551537	2
CAGTACG	780	0.0	28.32244	9
TCTCTGC	3030	0.0	28.228477	8
AGGGCTA	5440	0.0	28.163647	5
AGACAGT	1185	0.0	28.1578	6
AACGCAG	18870	0.0	27.071753	6
CTGTGCG	6475	0.0	26.786377	9
>>END_MODULE