FastQCFastQC Report
Mon 16 Jan 2017
ERR1146474_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146474_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5701384
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC196850.3452670439317892No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN174850.3066799219277284No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT158440.27789743683288126No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA132510.23241725167082236No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC120270.21094878015583585No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC107530.1886033285953025No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA106900.18749833373791347No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG91770.1609609175596662No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC91390.16029441272505063No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC89590.15713728456108203No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT84840.1488059741283871No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT75310.13209073446026437No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC73620.1291265419063161No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT72030.12633774536147713No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG71010.1245487060685616No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT66950.1174276280987213No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT66810.11718207368596818No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC66750.11707683608050257No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG62840.1102188521243263No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG60750.10655307553394053No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA60150.10550069947928432No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA60140.1054831598783734No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA57790.10136135366430325No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT57670.10115087845337202No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC59300.035.75930494
GTACTAG61900.032.7710081
CAACGCA102050.031.9938495
GTATCAA121350.030.4781271
ATCAACG110150.029.729333
GCATCGT62800.029.6060928
TAGGCAT74650.029.0112085
TACCCCG5700.028.0219755
CTGTGCG86000.026.2071049
CTAGGCA86450.026.1936444
TATCAAC142750.025.7697982
TAGTACT89150.025.7692284
ACGCAGA126850.025.3167157
CGCAGAG127400.025.0602288
TATTCTC30950.024.8930385
GGTATCA82650.024.8289531
AACGCAG139650.024.7416576
ACTGTGC96950.024.117218
GTACATG220900.024.0466561
GTACTGT97650.023.7490716
Produced by FastQC (version 0.11.5)