##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146474_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5701384
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.369313661384673	31.0	30.0	34.0	26.0	34.0
2	30.48367185932398	31.0	30.0	34.0	26.0	34.0
3	30.692900004630456	31.0	31.0	34.0	26.0	34.0
4	34.15972595425953	37.0	35.0	37.0	30.0	37.0
5	34.36435591779119	37.0	35.0	37.0	30.0	37.0
6	34.50275845303526	37.0	35.0	37.0	30.0	37.0
7	34.369602714007684	37.0	35.0	37.0	30.0	37.0
8	34.32375910831475	37.0	35.0	37.0	30.0	37.0
9	35.70842939188099	39.0	35.0	39.0	30.0	39.0
10-11	35.629883901873654	39.0	35.0	39.0	29.5	39.0
12-13	35.61717628561767	39.0	35.0	39.0	30.0	39.0
14-15	36.60986104426574	40.0	36.0	41.0	28.5	41.0
16-17	36.43229696508777	39.5	36.0	41.0	27.5	41.0
18-19	36.374060315881195	39.5	36.0	41.0	27.5	41.0
20-21	36.70152764662054	40.0	36.0	41.0	29.0	41.0
22-23	36.40802242052105	39.5	35.5	41.0	27.5	41.0
24-25	36.50406883661932	39.0	35.5	41.0	28.5	41.0
26-27	36.56115900981236	39.0	35.0	41.0	29.0	41.0
28-29	36.38407121849713	39.0	35.0	41.0	28.5	41.0
30-31	36.3202036558141	39.0	35.0	41.0	28.5	41.0
32-33	36.415298110072925	39.0	35.0	41.0	29.0	41.0
34-35	36.45680811887078	39.0	35.0	41.0	29.0	41.0
36-37	36.387374714630695	39.0	35.0	41.0	29.0	41.0
38-39	36.21981610079237	39.0	35.0	41.0	28.0	41.0
40-41	36.18059886862558	39.0	35.0	41.0	28.0	41.0
42-43	36.02095096559011	39.0	35.0	41.0	28.0	41.0
44-45	35.83737922230813	39.0	34.0	41.0	27.0	41.0
46-47	35.70511046089862	38.0	34.0	40.0	27.0	41.0
48-49	35.57009157776427	38.0	34.0	40.0	27.0	41.0
50-51	35.35154499328584	38.0	33.5	40.0	26.5	41.0
52-53	35.155826462487	38.0	33.0	40.0	26.0	41.0
54-55	34.95155325443787	37.0	33.0	40.0	26.0	41.0
56-57	34.80043819184956	37.0	33.0	40.0	26.0	41.0
58-59	34.531233995114164	36.5	33.0	40.0	26.0	41.0
60-61	34.27516494240697	36.0	33.0	39.5	26.0	41.0
62-63	33.99781044392029	35.5	32.0	39.0	26.0	41.0
64-65	33.715352623152555	35.0	32.0	39.0	25.0	41.0
66-67	33.42457875140492	35.0	32.0	38.5	25.0	40.0
68-69	33.127897103580466	35.0	31.5	38.0	24.0	40.0
70-71	33.06921459771873	35.0	32.0	37.0	25.0	40.0
72-73	32.861185634926535	35.0	31.5	37.0	25.0	40.0
74-75	32.60679792836265	35.0	31.0	36.0	24.0	39.0
76-77	32.38166487295015	35.0	31.0	36.0	24.0	39.0
78-79	32.16615439338939	35.0	31.0	36.0	24.0	38.5
80-81	31.94936580311026	35.0	31.0	35.0	23.5	37.0
82-83	31.72381048180582	34.5	31.0	35.0	23.0	37.0
84-85	31.551203883127325	34.0	31.0	35.0	23.0	36.5
86-87	31.393537779598777	34.0	31.0	35.0	23.0	36.0
88-89	31.41183228493292	35.0	31.0	35.0	23.0	36.0
90-91	31.34024475460695	34.5	31.0	35.0	23.0	36.0
92-93	31.204771683507023	34.0	31.0	35.0	23.0	35.5
94-95	31.189502057745976	34.0	31.0	35.0	23.0	35.0
96-97	31.09927624590801	34.0	31.0	35.0	22.5	35.0
98-99	31.023936994947192	34.0	31.0	35.0	21.5	35.0
100	30.519747310477598	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8228875475440702
1101	2	0.901189333924961
1101	3	0.8737324807868063
1101	4	0.9217361698342188
1101	5	0.8522368109867955
1101	6	0.6010307032903555
1101	7	0.6131807108134311
1101	8	0.6749348240573099
1101	9	0.8786555296268332
1101	10-11	0.8394557587462828
1101	12-13	0.7339262726226607
1101	14-15	0.9386483883051966
1101	16-17	1.0978820594591454
1101	18-19	1.2332825597002852
1101	20-21	0.8219466755672258
1101	22-23	1.1895434238298037
1101	24-25	1.0411898254971703
1101	26-27	0.9558460458562337
1101	28-29	1.1507995525844308
1101	30-31	1.1259492846127799
1101	32-33	0.9383736459283298
1101	34-35	0.8374024057307068
1101	36-37	0.8527767146732188
1101	38-39	0.9736707303897916
1101	40-41	0.8893192966875247
1101	42-43	0.8862354097099114
1101	44-45	0.8984054388295704
1101	46-47	0.8966541386306801
1101	48-49	0.9274801209835459
1101	50-51	0.9602438949069878
1101	52-53	0.9278776678042036
1101	54-55	0.9387852585757557
1101	56-57	0.8293975468352954
1101	58-59	0.8294247438319502
1101	60-61	0.8811924994403171
1101	62-63	0.8411606286797877
1101	64-65	0.7770651036213891
1101	66-67	0.8019559863123717
1101	68-69	0.7996674743241954
1101	70-71	0.7105442040326224
1101	72-73	0.7158517148244243
1101	74-75	0.7477762202526392
1101	76-77	0.7048517245897159
1101	78-79	0.685144908737584
1101	80-81	0.7390266030523875
1101	82-83	0.7527904545132635
1101	84-85	0.7680312811073335
1101	86-87	0.7511040494755505
1101	88-89	0.720925527865866
1101	90-91	0.7620384366932278
1101	92-93	0.848571790618557
1101	94-95	0.8390444665487315
1101	96-97	0.791094026101625
1101	98-99	0.8177444243448946
1101	100	0.8717554481843734
1102	1	0.5792132122087139
1102	2	0.6958140972430229
1102	3	0.6932653558979709
1102	4	0.7342416773428369
1102	5	0.6422777219423779
1102	6	0.44034078465325877
1102	7	0.4870693661208918
1102	8	0.5357513149085662
1102	9	0.6635354567346496
1102	10-11	0.6206906244438386
1102	12-13	0.5733479469228584
1102	14-15	0.7487954686755884
1102	16-17	0.9712195148326401
1102	18-19	0.9322972664888383
1102	20-21	0.6560122009984823
1102	22-23	0.9712908628518377
1102	24-25	0.8460491370535337
1102	26-27	0.710670636141856
1102	28-29	0.8537759264887868
1102	30-31	0.8946784287756557
1102	32-33	0.7077923935494468
1102	34-35	0.6248383646403042
1102	36-37	0.6517411559108481
1102	38-39	0.7041861761224553
1102	40-41	0.638778317135305
1102	42-43	0.634592699121221
1102	44-45	0.7231932272053356
1102	46-47	0.6900154920530639
1102	48-49	0.6073531642868488
1102	50-51	0.651049523553084
1102	52-53	0.6062149848888296
1102	54-55	0.5731115289086901
1102	56-57	0.5226016560695612
1102	58-59	0.4980351430197487
1102	60-61	0.5788379680861269
1102	62-63	0.5754574181957608
1102	64-65	0.4856792490297508
1102	66-67	0.49481264715587514
1102	68-69	0.5931398856187045
1102	70-71	0.5078271265320851
1102	72-73	0.4760188989946599
1102	74-75	0.47139252292912204
1102	76-77	0.4269170071013946
1102	78-79	0.4259783473959473
1102	80-81	0.4891588692517388
1102	82-83	0.5141949450426146
1102	84-85	0.42554718040964445
1102	86-87	0.46481869725788627
1102	88-89	0.5004363498838131
1102	90-91	0.5013408118437006
1102	92-93	0.5749909684957615
1102	94-95	0.5592183446390493
1102	96-97	0.4976974348158336
1102	98-99	0.5164662593901355
1102	100	0.6459407601842493
1103	1	0.6501855703564416
1103	2	0.8089571631775456
1103	3	0.766689865995442
1103	4	0.73899407710487
1103	5	0.6773222973272013
1103	6	0.5349427676346394
1103	7	0.5976915869199004
1103	8	0.6211485538372585
1103	9	0.7183926012687749
1103	10-11	0.7740075301069567
1103	12-13	0.7369438788526637
1103	14-15	0.8171401501491786
1103	16-17	1.1078403793762561
1103	18-19	1.1341942528369628
1103	20-21	0.8470874849123504
1103	22-23	1.0019153823404068
1103	24-25	0.9377650842636172
1103	26-27	0.8173118725651847
1103	28-29	1.0280242496803353
1103	30-31	1.0741459667264337
1103	32-33	0.8651402517127948
1103	34-35	0.7951359076811357
1103	36-37	0.786761371834956
1103	38-39	0.8547852858847378
1103	40-41	0.8037496385743026
1103	42-43	0.8878749803021933
1103	44-45	0.9010645640632831
1103	46-47	0.8619770658381043
1103	48-49	0.8416310211332103
1103	50-51	0.8860715457573676
1103	52-53	0.8760272358938934
1103	54-55	0.8539320252516305
1103	56-57	0.7623710591481085
1103	58-59	0.7013157906274188
1103	60-61	0.6943454430864122
1103	62-63	0.6988153036943245
1103	64-65	0.7512649537518499
1103	66-67	0.8217743498867236
1103	68-69	0.8188946646581741
1103	70-71	0.766398873915584
1103	72-73	0.6763371886761362
1103	74-75	0.6345050509118622
1103	76-77	0.6569976388771579
1103	78-79	0.6999272007612376
1103	80-81	0.6708090585138038
1103	82-83	0.5984735628380697
1103	84-85	0.5758660830057494
1103	86-87	0.6505610915262778
1103	88-89	0.7047516869789376
1103	90-91	0.7139381902779895
1103	92-93	0.7518962116609345
1103	94-95	0.6564694952628933
1103	96-97	0.6912455955044905
1103	98-99	0.7310599475070241
1103	100	0.7808488270159408
1104	1	0.4960143751421562
1104	2	0.6118526361638992
1104	3	0.5726925629906212
1104	4	0.673993041798937
1104	5	0.592962774400398
1104	6	0.36852029735375424
1104	7	0.3923629402748787
1104	8	0.3749709904930043
1104	9	0.47720742805542216
1104	10-11	0.5487251357511838
1104	12-13	0.4323940829484769
1104	14-15	0.6157292001116161
1104	16-17	0.8662079384273085
1104	18-19	0.6903587265884426
1104	20-21	0.6094674981298027
1104	22-23	0.8384987971639433
1104	24-25	0.7161373462135217
1104	26-27	0.6025063396431207
1104	28-29	0.7623749782214517
1104	30-31	0.82156229879741
1104	32-33	0.6512257500722356
1104	34-35	0.5682444559973376
1104	36-37	0.555538942679938
1104	38-39	0.6651359161075945
1104	40-41	0.5497765971218058
1104	42-43	0.5257853287620833
1104	44-45	0.5906663760835329
1104	46-47	0.5316414013938839
1104	48-49	0.494337056872574
1104	50-51	0.5449014794950315
1104	52-53	0.5980462248841789
1104	54-55	0.5671026966963169
1104	56-57	0.46679818078235513
1104	58-59	0.4252617259796665
1104	60-61	0.5709569841187587
1104	62-63	0.49178511548969084
1104	64-65	0.4314391642271431
1104	66-67	0.5751429060618634
1104	68-69	0.4738766087871227
1104	70-71	0.4384295548419388
1104	72-73	0.4524005664301214
1104	74-75	0.4839189557223378
1104	76-77	0.5033339985325256
1104	78-79	0.5069792455639259
1104	80-81	0.5023526950071044
1104	82-83	0.43625313092044493
1104	84-85	0.38700074112708194
1104	86-87	0.3802135055524971
1104	88-89	0.36934019752224145
1104	90-91	0.3605642308684658
1104	92-93	0.3614291612046969
1104	94-95	0.31210197524775296
1104	96-97	0.34267049196573396
1104	98-99	0.3947237727322097
1104	100	0.40591874439166986
1105	1	0.4155469786273791
1105	2	0.5831161544359738
1105	3	0.5896160485724096
1105	4	0.67508639410417
1105	5	0.5986848142978616
1105	6	0.2672749018229581
1105	7	0.31574702257177023
1105	8	0.361257272073388
1105	9	0.3551280163498234
1105	10-11	0.45934413374422434
1105	12-13	0.45456080419928924
1105	14-15	0.585092822613106
1105	16-17	0.7186212164701544
1105	18-19	0.7597968675776698
1105	20-21	0.5892529458736249
1105	22-23	0.8261155695327176
1105	24-25	0.6866208061204304
1105	26-27	0.5030046061643247
1105	28-29	0.7091860259795411
1105	30-31	0.7301041469888858
1105	32-33	0.5581808484028343
1105	34-35	0.49180749467445395
1105	36-37	0.41549938871862935
1105	38-39	0.5722826582546148
1105	40-41	0.39779709246916184
1105	42-43	0.4760094757299882
1105	44-45	0.5377197372197671
1105	46-47	0.45698102037628985
1105	48-49	0.517573198852304
1105	50-51	0.6037945661276964
1105	52-53	0.5653445893570748
1105	54-55	0.4666285270520163
1105	56-57	0.4654502964256011
1105	58-59	0.44813637163042586
1105	60-61	0.4464033126430209
1105	62-63	0.36093563223842295
1105	64-65	0.4170688879456108
1105	66-67	0.4596012741057436
1105	68-69	0.482717148956354
1105	70-71	0.4307148719797951
1105	72-73	0.41567169686785377
1105	74-75	0.4019188034237615
1105	76-77	0.41364308261225347
1105	78-79	0.41386740949601375
1105	80-81	0.48820194159481645
1105	82-83	0.4356102041883325
1105	84-85	0.44344196991878704
1105	86-87	0.5319000210973428
1105	88-89	0.5046167386673339
1105	90-91	0.5398080984051177
1105	92-93	0.5141435379523784
1105	94-95	0.5248298681549848
1105	96-97	0.5792871619098356
1105	98-99	0.574419675672253
1105	100	0.6437456551211049
1106	1	0.45044329390715276
1106	2	0.4869778028104257
1106	3	0.4604755023478724
1106	4	0.6092043519737373
1106	5	0.47794032637638395
1106	6	0.20445599273133297
1106	7	0.2040397058279666
1106	8	0.3153622795193556
1106	9	0.4240668691490228
1106	10-11	0.361565391037729
1106	12-13	0.24124090252625763
1106	14-15	0.4456350953602737
1106	16-17	0.545914231129025
1106	18-19	0.5200035084110084
1106	20-21	0.32923115763606603
1106	22-23	0.6493214727357568
1106	24-25	0.4619443380614996
1106	26-27	0.31372585415842735
1106	28-29	0.5719545157046824
1106	30-31	0.5660831343772301
1106	32-33	0.4733410078468623
1106	34-35	0.40643713975194373
1106	36-37	0.2933568836028755
1106	38-39	0.44155148966395785
1106	40-41	0.4388642004986423
1106	42-43	0.420059250828551
1106	44-45	0.4407563469955349
1106	46-47	0.3936498765472294
1106	48-49	0.41548368010803927
1106	50-51	0.43892372263329804
1106	52-53	0.3999647152778181
1106	54-55	0.4294703992371396
1106	56-57	0.3576850940259746
1106	58-59	0.38395424907085385
1106	60-61	0.3127322603999332
1106	62-63	0.2760439236952692
1106	64-65	0.35033211049199053
1106	66-67	0.4466479348539423
1106	68-69	0.371590612490003
1106	70-71	0.3451422994276854
1106	72-73	0.3336369998238595
1106	74-75	0.3566892126940999
1106	76-77	0.3037039608974794
1106	78-79	0.3387644361958735
1106	80-81	0.41522287336607633
1106	82-83	0.4948991438035044
1106	84-85	0.41627956437606883
1106	86-87	0.41177840938895116
1106	88-89	0.4623672185382901
1106	90-91	0.3526636005151609
1106	92-93	0.38963495030170847
1106	94-95	0.41599167362499045
1106	96-97	0.4180870864587938
1106	98-99	0.45829364223329705
1106	100	0.36559202779019984
1107	1	0.3373764903405636
1107	2	0.5283710647399857
1107	3	0.4391735397341243
1107	4	0.5329776525281176
1107	5	0.4391091785188337
1107	6	0.20551409690352784
1107	7	0.21074918278705468
1107	8	0.20239390002317492
1107	9	0.3700928746849712
1107	10-11	0.3830557182114802
1107	12-13	0.25106766853257056
1107	14-15	0.4179752793213609
1107	16-17	0.5249793150901354
1107	18-19	0.6993302935825838
1107	20-21	0.38453278708307437
1107	22-23	0.570601635998365
1107	24-25	0.32789740296853154
1107	26-27	0.3399433706273811
1107	28-29	0.4942025338257565
1107	30-31	0.5103294408859185
1107	32-33	0.48553669117568177
1107	34-35	0.30258682935643577
1107	36-37	0.305289321600533
1107	38-39	0.30734561913502745
1107	40-41	0.1814776266100111
1107	42-43	0.3397732033760903
1107	44-45	0.3167177846700113
1107	46-47	0.2870214568421403
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2210	4	-0.025461647305036195
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2210	22-23	-1.878657685458208
2210	24-25	-0.9434813472539503
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2210	28-29	0.03933133692547841
2210	30-31	0.0673633332549457
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2212	98-99	-0.3915381014614283
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2214	2	-0.838072794814348
2214	3	-1.1147611927043783
2214	4	-0.7293797419150891
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2214	7	-1.0634552980543646
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2214	16-17	-3.0533056425996676
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2214	26-27	-1.3845700878484166
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2214	30-31	-1.018185682211147
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2214	96-97	-0.9644461043101629
2214	98-99	-1.1906236958861243
2214	100	-1.0093539377652299
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2215	2	-2.1131336306362165
2215	3	-3.7555013573540243
2215	4	-1.903185029347405
2215	5	-1.4969066088913223
2215	6	-1.342451773769163
2215	7	-1.1011584680207065
2215	8	-1.085629638877073
2215	9	-1.5754544491943676
2215	10-11	-1.2547775114642548
2215	12-13	-1.1732940637356535
2215	14-15	-3.5641594402935155
2215	16-17	-3.2685218468779595
2215	18-19	-2.132014704885819
2215	20-21	-1.6770144727531147
2215	22-23	-3.123603056998128
2215	24-25	-5.537215223383598
2215	26-27	-3.622522115618821
2215	28-29	-1.6353737959803851
2215	30-31	-4.023889060834875
2215	32-33	-4.020252729736292
2215	34-35	-2.9233012595173093
2215	36-37	-1.8072927430325691
2215	38-39	-1.6806198374744596
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2215	44-45	-3.2450735687775634
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2215	50-51	-5.171027828818509
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2215	54-55	-3.0385095838156957
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2215	64-65	-1.674749835057419
2215	66-67	-1.5126333194152224
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2215	70-71	-1.4028578362925401
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2215	80-81	-1.4224824816003476
2215	82-83	-1.427554695694834
2215	84-85	-1.2828624070137522
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2215	90-91	-1.6016778840676444
2215	92-93	-3.802568637176673
2215	94-95	-1.9544045312089686
2215	96-97	-1.522500983912142
2215	98-99	-3.0336974359478006
2215	100	-3.071338784361405
2216	1	-0.5496757057573447
2216	2	-0.4341643623392457
2216	3	-0.6816286032973835
2216	4	-0.6051728077151495
2216	5	-0.7103077811682397
2216	6	-0.6104772225674608
2216	7	-0.7551047078169901
2216	8	-0.7026360216229008
2216	9	-0.7688941631658253
2216	10-11	-0.867322531521765
2216	12-13	-0.9317065613657718
2216	14-15	-1.318743258668313
2216	16-17	-1.0591519527831252
2216	18-19	-3.0679233941389583
2216	20-21	-1.3606110918050405
2216	22-23	-0.7900094967700895
2216	24-25	-0.9532479524395541
2216	26-27	-0.9232248566486163
2216	28-29	-0.8975741628646077
2216	30-31	-0.9838103522369366
2216	32-33	-0.8902924461630377
2216	34-35	-1.2228360357765382
2216	36-37	-1.2293570567180225
2216	38-39	-1.2356957720710042
2216	40-41	-1.2454358965324133
2216	42-43	-1.20617428794327
2216	44-45	-1.1560693574628118
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2216	50-51	-1.1801250304338353
2216	52-53	-1.1075698008378723
2216	54-55	-0.9774085751845689
2216	56-57	-1.0744354717918583
2216	58-59	-1.139906124656136
2216	60-61	-1.1368069355971286
2216	62-63	-1.0971802892137603
2216	64-65	-1.2095154616024004
2216	66-67	-1.1801002798796532
2216	68-69	-1.267230591117297
2216	70-71	-1.3577938097923905
2216	72-73	-1.341915891992251
2216	74-75	-1.3580530047652388
2216	76-77	-1.249154262897406
2216	78-79	-1.3807821936610054
2216	80-81	-1.372046623596141
2216	82-83	-1.4848825128035692
2216	84-85	-1.3173352108847851
2216	86-87	-1.3703371981095245
2216	88-89	-1.2062031411594276
2216	90-91	-1.2786624097062145
2216	92-93	-1.2137412007130166
2216	94-95	-1.1665960298153948
2216	96-97	-1.1283601751796546
2216	98-99	-1.1786867280095947
2216	100	-1.1593663422328895
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	23421.0
1	3814.0
2	580.0
3	542.0
4	552.0
5	309.0
6	296.0
7	373.0
8	674.0
9	1143.0
10	2075.0
11	4140.0
12	7311.0
13	10760.0
14	14291.0
15	18157.0
16	21581.0
17	25845.0
18	29202.0
19	33193.0
20	37362.0
21	42275.0
22	48365.0
23	55948.0
24	63003.0
25	70724.0
26	81031.0
27	93054.0
28	110122.0
29	129393.0
30	153694.0
31	183693.0
32	223956.0
33	281605.0
34	381435.0
35	513057.0
36	751973.0
37	1116924.0
38	892232.0
39	273091.0
40	188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.757752372981976	20.895227037228434	12.535371732482789	23.811648857306807
2	15.42385115565819	20.128408618620732	40.539889142649955	23.90785108307113
3	18.673364127648423	27.006845798662702	28.687074054223917	25.632716019464958
4	12.339871008669908	16.98130330584338	40.33539508000282	30.343430605483896
5	13.35179254142532	38.550337953948635	34.820856838201195	13.27701266642486
6	29.46413763359899	38.47996462328789	18.559326051779507	13.496571691333603
7	26.736106826603844	31.624493600620777	24.011289475883032	17.628110096892343
8	25.831868718831036	34.77655300552998	20.42769681510248	18.9638814605365
9	26.00731218686645	15.249173072661176	21.852832057541203	36.89068268293118
10-11	25.575745980637944	25.759888770208118	27.632007130838275	21.032358118315667
12-13	26.013593099240733	23.57094367036602	27.48923519641138	22.926228033981864
14-15	23.41966677263729	24.798351654261403	25.00147671211766	26.780504860983644
16-17	22.469624740682878	27.22366470968385	26.36346804745779	23.943242502175487
18-19	21.817460796065482	28.07277488775827	27.837967415250485	22.271796900925757
20-21	23.614190833974796	27.200224005848415	26.694429943995896	22.491155216180896
22-23	24.14671640384907	26.689035884254324	26.448977922757898	22.715269789138706
24-25	23.536060687462935	26.793349847132603	27.040210487023657	22.630378978380804
26-27	23.389361704940747	27.156391566612854	26.027979369148653	23.426267359297746
28-29	22.444717509691955	27.571228599686542	26.827782018712217	23.156271871909283
30-31	23.28861879456303	27.13532968653103	26.776070735799884	22.799980783106058
32-33	23.565371054015586	27.42042092498559	25.801652945016844	23.21255507598198
34-35	23.585161753043547	27.1931140067243	26.707751282902407	22.51397295732975
36-37	23.335479930171964	26.809263245814908	26.275641551895163	23.579615272117966
38-39	23.510149063124743	26.80253945682273	26.898019492422527	22.789291987630005
40-41	23.16603916695125	26.00765661635471	27.525638960303557	23.300665256390484
42-43	23.07964149516503	26.27274656641835	26.88005937871894	23.767552559697684
44-45	23.25475864687651	27.103818072457127	26.698571310282286	22.942851970384076
46-47	23.143529553682747	26.862285921937186	26.23973462736998	23.754449897010087
48-49	23.89065440408154	26.34479643378175	26.64609522392315	23.11845393821356
50-51	23.504595885480754	26.287052486165436	26.5443995018817	23.66395212647211
52-53	22.973533634717576	26.848414682334322	26.381782798458296	23.79626888448981
54-55	22.953081759109786	26.75503320821015	26.412956946285377	23.87892808639469
56-57	23.986093668934284	26.238868168880725	26.11393687844567	23.661101283739317
58-59	22.817063681197798	26.612801551981125	27.087312868186874	23.482821898634203
60-61	22.943486747102664	27.16306185743328	26.404791249014824	23.488660146449234
62-63	23.4335779724963	26.48810599498474	25.912098804437534	24.166217228081425
64-65	22.46563714386197	26.670026917566968	26.492128169973096	24.37220776859797
66-67	23.446942657015654	26.23754445943507	26.37276867481415	23.942744208735125
68-69	22.6135450466462	26.388008430414995	26.70401231282903	24.294434210109777
70-71	22.635939501507334	26.071427028522514	26.740766126035325	24.551867343934834
72-73	23.16335448348246	26.18431312801078	27.123197061762173	23.529135326744587
74-75	23.347326926815548	26.482558998139233	26.725882652190798	23.44423142285442
76-77	22.95237426088514	26.450854928215513	26.317077486098384	24.279693324800963
78-79	22.917026565922214	26.48292382199297	26.35116872791387	24.24888088417095
80-81	22.499852992037415	26.76653099029236	27.106963522541765	23.62665249512846
82-83	23.34007234831574	25.735913836527224	28.012050529564565	22.911963285592474
84-85	23.445397214762494	26.24636385093193	26.7381019577389	23.570136976566683
86-87	22.174192366118398	26.88144255131577	27.208385779389072	23.735979303176762
88-89	23.20515565087423	25.48604926817476	27.136941588953707	24.171853491997304
90-91	22.829564287481414	25.707783449893988	27.171943153513357	24.290709109111237
92-93	23.028487812421748	25.91462700924257	27.219566168891653	23.837319009444034
94-95	21.99002534499872	25.851739073411107	27.908185855520294	24.25004972606988
96-97	22.33677746866029	25.77572848893273	27.389756265171076	24.497737777235898
98-99	21.741555693660683	26.3862267593766	27.295808324192418	24.576409222770298
100	22.530283067549284	25.532629107564958	26.629877165387843	25.307210659497915
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19448.0
1	11412.0
2	3093.5
3	2121.0
4	1032.0
5	531.5
6	402.5
7	369.5
8	348.5
9	333.0
10	308.0
11	299.0
12	300.0
13	285.0
14	302.5
15	343.0
16	374.0
17	447.5
18	544.5
19	644.0
20	833.5
21	1135.5
22	1614.0
23	2319.5
24	3125.5
25	4307.0
26	6410.5
27	8920.0
28	13146.0
29	22256.0
30	34918.5
31	48344.5
32	66217.0
33	84936.0
34	102747.5
35	119990.5
36	137049.5
37	164546.5
38	176983.0
39	170727.5
40	162766.0
41	153834.0
42	154014.0
43	166057.0
44	184160.0
45	216690.0
46	249802.0
47	271567.5
48	282698.0
49	275187.0
50	262710.0
51	266437.5
52	268552.5
53	257377.5
54	241600.5
55	209131.5
56	183188.5
57	152164.5
58	119433.0
59	110229.5
60	90739.0
61	63059.5
62	46884.5
63	38556.5
64	28068.5
65	14180.0
66	7667.5
67	5213.0
68	3893.5
69	2934.0
70	2202.5
71	1681.5
72	1260.0
73	989.0
74	759.0
75	547.5
76	393.5
77	277.0
78	190.0
79	128.5
80	89.5
81	58.5
82	48.0
83	45.5
84	30.0
85	18.5
86	19.0
87	20.0
88	17.5
89	12.0
90	8.0
91	6.0
92	6.0
93	6.5
94	6.5
95	5.0
96	3.0
97	3.5
98	2.0
99	1.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.994372594443735
2	0.44622498677514094
3	0.4804622877532894
4	0.4662727506163416
5	0.4336315533210884
6	0.4840052871372986
7	0.499703229952587
8	0.5029305165201993
9	0.5171200536571471
10-11	0.5304238409480926
12-13	0.5283629378410575
14-15	0.5262055669290123
16-17	0.5303799919458152
18-19	0.5286786506574545
20-21	0.5280033760223833
22-23	0.49045109047206786
24-25	0.5255829110966741
26-27	0.5053422116454531
28-29	0.511349524957449
30-31	0.5137612200827028
32-33	0.5164886280243534
34-35	0.5290206728752177
36-37	0.5466830509925309
38-39	0.5360979018427806
40-41	0.5413334727146952
42-43	0.5378606317343297
44-45	0.5482616150745152
46-47	0.5466830509925309
48-49	0.5192686547687368
50-51	0.5339405309307355
52-53	0.5261354085253686
54-55	0.5362294488496127
56-57	0.5238464906064914
58-59	0.5293626950929808
60-61	0.5225748695404484
62-63	0.5172954496662565
64-65	0.5293539252925255
66-67	0.527284252385035
68-69	0.5472443182216808
70-71	0.530879870571777
72-73	0.5349929069853916
74-75	0.5320901030346316
76-77	0.520057936809729
78-79	0.5506470007984026
80-81	0.5248287082575038
82-83	0.5249953344661576
84-85	0.53280922667198
86-87	0.841129101284881
88-89	1.1088711091903298
90-91	1.0995400415057115
92-93	1.0995926603084445
94-95	1.1048808499830918
96-97	1.0974791383986766
98-99	1.0938133618082908
100	1.0891039789637043
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5701384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.03586161662804
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.28797263567152	21.27979431612581
2	12.826822399463033	7.448756803437469
3	5.232423908492653	4.557838095795861
4	2.690556798104972	3.124905394458152
5	1.6065683737428207	2.3324048488823848
6	0.9488385523986784	1.6530206942381824
7	0.628190276056472	1.2768032147140962
8	0.45053627324525214	1.0465367106576369
9	0.32769981747211635	0.8563541896723176
>10	1.5623479774724514	8.146099674785468
>50	0.15485125639653524	3.2071141674449457
>100	0.2028462028736656	13.254153976011358
>500	0.04325368313925094	8.766377864751863
>1k	0.03485666911494663	17.950625728774273
>5k	0.0018123051532901193	3.3498011949939537
>10k+	4.2287120243436117E-4	1.749413125256433
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	19685	0.3452670439317892	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	17485	0.3066799219277284	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	15844	0.27789743683288126	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	13251	0.23241725167082236	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	12027	0.21094878015583585	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	10753	0.1886033285953025	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	10690	0.18749833373791347	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	9177	0.1609609175596662	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	9139	0.16029441272505063	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	8959	0.15713728456108203	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	8484	0.1488059741283871	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7531	0.13209073446026437	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	7362	0.1291265419063161	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	7203	0.12633774536147713	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	7101	0.1245487060685616	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	6695	0.1174276280987213	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6681	0.11718207368596818	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	6675	0.11707683608050257	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	6284	0.1102188521243263	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	6075	0.10655307553394053	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	6015	0.10550069947928432	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	6014	0.1054831598783734	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5779	0.10136135366430325	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	5767	0.10115087845337202	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	0.0	0.0
18-19	0.0	0.0	0.0	0.0	0.0
20-21	0.0	0.0	0.0	8.769800455468356E-6	0.0
22-23	0.0	0.0	0.0	6.138860318827849E-5	0.0
24-25	0.0	0.0	0.0	1.3154700683202535E-4	0.0
26-27	0.0	0.0	0.0	4.2972022231794945E-4	0.0
28-29	0.0	0.0	0.0	0.0012979304674093166	0.0
30-31	0.0	0.0	0.0	0.006209018722471597	0.0
32-33	0.0	0.0	0.0	0.017864083527789044	0.0
34-35	0.0	0.0	0.0	0.03566677845238981	0.0
36-37	0.0	0.0	0.0	0.06779932732122587	0.0
38-39	0.0	0.0	0.0	0.11929559559573605	0.0
40-41	0.0	0.0	0.0	0.17589588773532883	0.0
42-43	0.0	0.0	0.0	0.2375125057354495	0.0
44-45	0.0	0.0	0.0	0.314555202736739	0.0
46-47	0.0	0.0	0.0	0.4134434726726002	0.0
48-49	0.0	0.0	0.0	0.5281349230292154	0.0
50-51	0.0	0.0	0.0	0.6541394159733847	0.0
52-53	0.0	0.0	0.0	0.7801351391170985	0.0
54-55	0.0	0.0	0.0	0.9225391589129938	0.0
56-57	0.0	0.0	0.0	1.0990752420815717	0.0
58-59	0.0	0.0	0.0	1.3080508171349272	0.0
60-61	0.0	0.0	0.0	1.5311545407220422	0.0
62-63	0.0	0.0	0.0	1.7672989575864388	0.0
64-65	0.0	0.0	0.0	2.0194394904816093	0.0
66-67	0.0	0.0	0.0	2.3065627573936434	0.0
68-69	0.0	0.0	0.0	2.6287915355289173	0.0
70-71	0.0	0.0	0.0	2.992457620816279	0.0
72-73	0.0	0.0	0.0	3.3750226260851752	0.0
74-75	0.0	0.0	0.0	3.752395909484434	0.0
76-77	0.0	0.0	0.0	4.1878778907016265	0.0
78-79	0.0	0.0	0.0	4.6578690367110855	8.769800455468356E-6
80-81	0.0	0.0	0.0	5.167824163396116	1.753960091093671E-5
82-83	0.0	0.0	0.0	5.700089662439856	1.753960091093671E-5
84-85	0.0	0.0	0.0	6.22640923677479	1.753960091093671E-5
86-87	0.0	0.0	0.0	6.7926138635811935	1.753960091093671E-5
88	0.0	0.0	0.0	7.247485873605426	1.753960091093671E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	5930	0.0	35.759304	94
GTACTAG	6190	0.0	32.771008	1
CAACGCA	10205	0.0	31.993849	5
GTATCAA	12135	0.0	30.478127	1
ATCAACG	11015	0.0	29.72933	3
GCATCGT	6280	0.0	29.606092	8
TAGGCAT	7465	0.0	29.011208	5
TACCCCG	570	0.0	28.021975	5
CTGTGCG	8600	0.0	26.207104	9
CTAGGCA	8645	0.0	26.193644	4
TATCAAC	14275	0.0	25.769798	2
TAGTACT	8915	0.0	25.769228	4
ACGCAGA	12685	0.0	25.316715	7
CGCAGAG	12740	0.0	25.060228	8
TATTCTC	3095	0.0	24.893038	5
GGTATCA	8265	0.0	24.828953	1
AACGCAG	13965	0.0	24.741657	6
ACTGTGC	9695	0.0	24.11721	8
GTACATG	22090	0.0	24.046656	1
GTACTGT	9765	0.0	23.749071	6
>>END_MODULE