##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146474_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5701384
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.841998539302036	31.0	31.0	34.0	30.0	34.0
2	31.80983020964734	31.0	31.0	34.0	30.0	34.0
3	31.987475321781517	33.0	31.0	34.0	30.0	34.0
4	35.72179298921104	37.0	35.0	37.0	35.0	37.0
5	35.640673387374015	37.0	35.0	37.0	33.0	37.0
6	35.67710980351438	37.0	35.0	37.0	33.0	37.0
7	35.598220712725194	37.0	35.0	37.0	33.0	37.0
8	35.584668740081355	37.0	35.0	37.0	33.0	37.0
9	37.17068610007675	39.0	37.0	39.0	33.0	39.0
10-11	37.120128551243	39.0	37.0	39.0	33.0	39.0
12-13	37.05825883680173	39.0	37.0	39.0	33.0	39.0
14-15	38.266911858594334	40.0	38.0	41.0	33.0	41.0
16-17	38.26474185916963	40.0	38.0	41.0	33.0	41.0
18-19	38.30412668222313	40.0	38.0	41.0	33.0	41.0
20-21	38.31219823116633	40.0	38.0	41.0	33.0	41.0
22-23	38.277588301366826	40.0	38.0	41.0	33.0	41.0
24-25	38.209931395605	40.0	37.0	41.0	33.0	41.0
26-27	38.07539599156976	40.0	37.0	41.0	33.0	41.0
28-29	38.01055971672843	40.0	37.0	41.0	33.0	41.0
30-31	37.896777080793015	40.0	37.0	41.0	33.0	41.0
32-33	37.79675969904851	40.0	37.0	41.0	33.0	41.0
34-35	37.714379526094014	40.0	36.5	41.0	32.0	41.0
36-37	37.63846795795547	40.0	36.0	41.0	32.0	41.0
38-39	37.525223700070015	40.0	36.0	41.0	32.0	41.0
40-41	37.40849809449776	40.0	36.0	41.0	31.0	41.0
42-43	37.28918916880533	39.5	36.0	41.0	31.0	41.0
44-45	37.106958591107	39.0	35.5	41.0	31.0	41.0
46-47	36.90027886211488	39.0	35.0	41.0	30.5	41.0
48-49	36.66975290561029	39.0	35.0	41.0	30.0	41.0
50-51	36.46530079363186	39.0	35.0	41.0	30.0	41.0
52-53	36.26397081831359	38.5	35.0	40.5	30.0	41.0
54-55	36.06140482731912	38.0	35.0	40.0	29.0	41.0
56-57	35.800664628097316	38.0	34.5	40.0	29.0	41.0
58-59	35.51579748005045	37.0	34.0	40.0	28.5	41.0
60-61	35.25856572719887	37.0	34.0	40.0	28.0	41.0
62-63	34.973180196247085	36.0	34.0	39.5	28.0	41.0
64-65	34.70057270304894	35.5	34.0	39.0	28.0	41.0
66-67	34.46991528723552	35.0	33.0	39.0	28.0	41.0
68-69	34.27139919009139	35.0	33.0	38.5	28.0	41.0
70-71	34.06093257356459	35.0	33.0	37.5	27.5	40.0
72-73	33.85486348577819	35.0	33.0	37.0	27.0	40.0
74-75	33.67448798397021	35.0	33.0	37.0	27.0	39.0
76-77	33.516332174784225	35.0	33.0	36.0	27.0	39.0
78-79	33.39659922923978	35.0	33.0	36.0	27.0	39.0
80-81	33.25095318961151	35.0	33.0	36.0	27.0	37.5
82-83	33.06290376161297	35.0	33.0	35.0	27.0	37.0
84-85	32.92803492976442	35.0	33.0	35.0	27.0	37.0
86-87	32.80082529084166	35.0	33.0	35.0	27.0	36.0
88-89	32.69656902604701	35.0	33.0	35.0	27.0	36.0
90-91	32.603923363169365	35.0	33.0	35.0	27.0	36.0
92-93	32.52868663117587	35.0	33.0	35.0	27.0	36.0
94-95	32.4936286522711	35.0	33.0	35.0	27.0	35.0
96-97	32.44307390977349	35.0	33.0	35.0	27.0	35.0
98-99	32.40620049447643	35.0	33.0	35.0	27.0	35.0
100	31.902544364666543	34.0	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4980080078283926
1101	2	0.546210301934952
1101	3	0.4663844999053346
1101	4	0.297422022723687
1101	5	0.35860082974856056
1101	6	0.39140801108568013
1101	7	0.39810664589278844
1101	8	0.3988601087327197
1101	9	0.45430203451976325
1101	10-11	0.5005518166118748
1101	12-13	0.4949985333876441
1101	14-15	0.6919551246546831
1101	16-17	0.6525510756560351
1101	18-19	0.6841428683842992
1101	20-21	0.6384324947599609
1101	22-23	0.6414447522549764
1101	24-25	0.7160776193303704
1101	26-27	0.7309125497409568
1101	28-29	0.7309733047372688
1101	30-31	0.7928191239264422
1101	32-33	0.7926413616117074
1101	34-35	0.7150186486942971
1101	36-37	0.7242928080330557
1101	38-39	0.7715305866065307
1101	40-41	0.728415261326468
1101	42-43	0.7807697541229288
1101	44-45	0.7546527394555369
1101	46-47	0.8026915721769114
1101	48-49	0.7966320816717882
1101	50-51	0.7722856840950669
1101	52-53	0.8072178161249397
1101	54-55	0.7314342898592301
1101	56-57	0.7567106588841739
1101	58-59	0.7955796247560443
1101	60-61	0.7912373073311656
1101	62-63	0.7644616520576903
1101	64-65	0.7357435518976772
1101	66-67	0.711243203983912
1101	68-69	0.7227549953730446
1101	70-71	0.6516799660857373
1101	72-73	0.6485671925964382
1101	74-75	0.6181895122096606
1101	76-77	0.5803316306132942
1101	78-79	0.5291059657560027
1101	80-81	0.5386395959431738
1101	82-83	0.5128811398106237
1101	84-85	0.5600110159161957
1101	86-87	0.5494051808786082
1101	88-89	0.5281592986641499
1101	90-91	0.5302170342151413
1101	92-93	0.538053779870836
1101	94-95	0.5321257756086268
1101	96-97	0.5512690753453668
1101	98-99	0.5838265083469878
1101	100	0.6054532234933241
1102	1	0.4124805282889348
1102	2	0.49768289747023076
1102	3	0.4002276407857899
1102	4	0.27978200690155575
1102	5	0.25947306368934875
1102	6	0.2746403056422153
1102	7	0.32169351743692687
1102	8	0.30961332749050996
1102	9	0.4011147347400339
1102	10-11	0.3910514873493369
1102	12-13	0.4275292549506773
1102	14-15	0.5214357721818885
1102	16-17	0.5318698462225271
1102	18-19	0.5376417824759017
1102	20-21	0.5262887014987427
1102	22-23	0.5047439327606256
1102	24-25	0.5614608814802793
1102	26-27	0.5665626260877517
1102	28-29	0.5508027970479361
1102	30-31	0.5858824141993964
1102	32-33	0.5926852840924326
1102	34-35	0.643047293688511
1102	36-37	0.6501428460054868
1102	38-39	0.5798352146489023
1102	40-41	0.5779066137242026
1102	42-43	0.6303831714477326
1102	44-45	0.6194004178173174
1102	46-47	0.6284574214665497
1102	48-49	0.6025249798931895
1102	50-51	0.5647538353917696
1102	52-53	0.6064786197691348
1102	54-55	0.6389891501867737
1102	56-57	0.6868015788142046
1102	58-59	0.6202352265552449
1102	60-61	0.5715047560898512
1102	62-63	0.5609457668454283
1102	64-65	0.5804153493552278
1102	66-67	0.5304288727576036
1102	68-69	0.49757522589670344
1102	70-71	0.5168775641883769
1102	72-73	0.5462943389406263
1102	74-75	0.5425913071188972
1102	76-77	0.5204128670103003
1102	78-79	0.47968857592645975
1102	80-81	0.4550967304518423
1102	82-83	0.4763920965630817
1102	84-85	0.5134189485452723
1102	86-87	0.5090745225781177
1102	88-89	0.415914193360706
1102	90-91	0.45375394086401144
1102	92-93	0.4647234230286088
1102	94-95	0.4307291612385953
1102	96-97	0.4129069358230666
1102	98-99	0.4250898705963877
1102	100	0.489248100858358
1103	1	0.2942742032021819
1103	2	-0.17111641342673778
1103	3	0.19201001131984796
1103	4	0.19470283430409552
1103	5	0.2547192225391086
1103	6	0.2863247308608763
1103	7	0.3164514871904771
1103	8	0.3349797212309653
1103	9	0.38180692415860307
1103	10-11	0.39022466941533906
1103	12-13	0.4466580866702117
1103	14-15	0.5346233575338246
1103	16-17	0.5890740512219992
1103	18-19	0.553661650207367
1103	20-21	0.5519903478523531
1103	22-23	0.5464588956713712
1103	24-25	0.5255890076987342
1103	26-27	0.5929148124931558
1103	28-29	0.563619757340966
1103	30-31	0.5884963652218502
1103	32-33	0.6599621306425334
1103	34-35	0.6557831207065448
1103	36-37	0.5867804916840598
1103	38-39	0.5894215370123419
1103	40-41	0.6581559086970756
1103	42-43	0.6702741744505758
1103	44-45	0.6929025875740464
1103	46-47	0.7176836562278623
1103	48-49	0.7260493197588147
1103	50-51	0.7030284461799425
1103	52-53	0.6701240900041157
1103	54-55	0.6374310679685848
1103	56-57	0.6626746036286519
1103	58-59	0.6417153909628368
1103	60-61	0.6214417544396866
1103	62-63	0.6380867341187226
1103	64-65	0.6317849966854538
1103	66-67	0.6458052333728759
1103	68-69	0.5599588776249007
1103	70-71	0.48949037664400663
1103	72-73	0.4967220572565907
1103	74-75	0.5133585193652834
1103	76-77	0.5076626326029228
1103	78-79	0.45863940607127063
1103	80-81	0.45849230344742153
1103	82-83	0.46443090533026776
1103	84-85	0.45482096010510276
1103	86-87	0.47351504047331616
1103	88-89	0.4649453376564736
1103	90-91	0.5083824500632019
1103	92-93	0.5205246833317076
1103	94-95	0.550815218867065
1103	96-97	0.5184521783109162
1103	98-99	0.5262524164120848
1103	100	0.48556557474008244
1104	1	0.32959728911268016
1104	2	0.38397427110829696
1104	3	0.30119047580658886
1104	4	0.20509832066665723
1104	5	0.23911689968588234
1104	6	0.23891268043239933
1104	7	0.24059947110455937
1104	8	0.23786153164556367
1104	9	0.28787603999176525
1104	10-11	0.2931301488099578
1104	12-13	0.3200052301113132
1104	14-15	0.4092241091284947
1104	16-17	0.4003823802487787
1104	18-19	0.44837925760996455
1104	20-21	0.4660397406993724
1104	22-23	0.4212242374219315
1104	24-25	0.46691974796519986
1104	26-27	0.5005848154916919
1104	28-29	0.553424606380851
1104	30-31	0.5228715590355719
1104	32-33	0.4865103689281298
1104	34-35	0.5044737243462976
1104	36-37	0.48643586656214666
1104	38-39	0.485463420393458
1104	40-41	0.5115510314026253
1104	42-43	0.5048951991012558
1104	44-45	0.5164598743807147
1104	46-47	0.47264045183003844
1104	48-49	0.43638201832723666
1104	50-51	0.5281141625527468
1104	52-53	0.5223143931753
1104	54-55	0.5093158904539976
1104	56-57	0.43417215412471677
1104	58-59	0.4740066569974246
1104	60-61	0.44218761982871513
1104	62-63	0.4355612382501377
1104	64-65	0.41159431551393055
1104	66-67	0.39290693568310786
1104	68-69	0.4289183297293988
1104	70-71	0.41067325718788794
1104	72-73	0.4141979321667151
1104	74-75	0.4005628803657473
1104	76-77	0.3249059536613714
1104	78-79	0.31430803196094814
1104	80-81	0.3139538367517005
1104	82-83	0.3041238300497966
1104	84-85	0.3143475041408692
1104	86-87	0.30788692226260395
1104	88-89	0.3345885526196213
1104	90-91	0.36515117171145306
1104	92-93	0.3547235284864385
1104	94-95	0.3294447498816453
1104	96-97	0.3149769905234052
1104	98-99	0.3186376188445976
1104	100	0.47032189753538134
1105	1	0.1295382299298069
1105	2	-0.3755649878223153
1105	3	0.03176508886723539
1105	4	0.09277260530095077
1105	5	0.1546031793862781
1105	6	0.24435083508976874
1105	7	0.26072106645695925
1105	8	0.2063957153179672
1105	9	0.27500610701115136
1105	10-11	0.3235493410854602
1105	12-13	0.337887452881553
1105	14-15	0.43077551747894205
1105	16-17	0.43860078072710706
1105	18-19	0.47043164390453285
1105	20-21	0.38834101785482034
1105	22-23	0.39166100608328946
1105	24-25	0.3926743515832314
1105	26-27	0.4438219105402723
1105	28-29	0.44236108429206666
1105	30-31	0.472688579313278
1105	32-33	0.48417628158155424
1105	34-35	0.5108920004125039
1105	36-37	0.5043331082468967
1105	38-39	0.506464383984806
1105	40-41	0.49810369363196827
1105	42-43	0.4824192177379345
1105	44-45	0.5317147184679172
1105	46-47	0.5380299005485369
1105	48-49	0.5881096739787921
1105	50-51	0.5507833420513748
1105	52-53	0.46506824308437444
1105	54-55	0.5046632712854517
1105	56-57	0.5895545932351709
1105	58-59	0.5785306599798972
1105	60-61	0.49937229414098994
1105	62-63	0.5137999512089806
1105	64-65	0.5032528634344544
1105	66-67	0.451940577151106
1105	68-69	0.5061378535978562
1105	70-71	0.4866135951266628
1105	72-73	0.4735059294198152
1105	74-75	0.4526509800970686
1105	76-77	0.4695298107759456
1105	78-79	0.4547818330096334
1105	80-81	0.46332318358755487
1105	82-83	0.43238548956620804
1105	84-85	0.4406700953503062
1105	86-87	0.44745646746655865
1105	88-89	0.44245120787216763
1105	90-91	0.47451645239979
1105	92-93	0.37708593354917497
1105	94-95	0.3952768217392375
1105	96-97	0.3621826724421382
1105	98-99	0.3649600528951211
1105	100	0.42412432093314933
1106	1	0.2700075916325311
1106	2	0.2982170214011042
1106	3	0.2331526219760427
1106	4	0.190258824836107
1106	5	0.14830839452517353
1106	6	0.1815146162083323
1106	7	0.21117703517018782
1106	8	0.24924477413497925
1106	9	0.24583582109455193
1106	10-11	0.23757276294977459
1106	12-13	0.23016631678725474
1106	14-15	0.30340799706520727
1106	16-17	0.27767766805123983
1106	18-19	0.34291573807986
1106	20-21	0.30299369354627004
1106	22-23	0.3207102830650328
1106	24-25	0.28699916512395873
1106	26-27	0.2767072272287123
1106	28-29	0.3026369642846376
1106	30-31	0.31110752889261306
1106	32-33	0.3571108599675554
1106	34-35	0.2818499910871921
1106	36-37	0.28452459503446903
1106	38-39	0.2602643401456177
1106	40-41	0.3169993425462181
1106	42-43	0.3018259167125237
1106	44-45	0.2736365740970257
1106	46-47	0.23746475432593428
1106	48-49	0.20502853875858307
1106	50-51	0.1990330061831429
1106	52-53	0.21406106191550833
1106	54-55	0.26657793414445763
1106	56-57	0.27895885349362715
1106	58-59	0.23318804000570736
1106	60-61	0.1991031760152424
1106	62-63	0.19841108156342102
1106	64-65	0.22806812286124512
1106	66-67	0.23363966683417203
1106	68-69	0.24257558724549
1106	70-71	0.2821949152608525
1106	72-73	0.19897142925933053
1106	74-75	0.19561999821274156
1106	76-77	0.17640304952785613
1106	78-79	0.17837410668060727
1106	80-81	0.19984442776368638
1106	82-83	0.21452515022925667
1106	84-85	0.17964869130414485
1106	86-87	0.1806908833308185
1106	88-89	0.19765991697986607
1106	90-91	0.16284838959422387
1106	92-93	0.1998475337324308
1106	94-95	0.22752458572258405
1106	96-97	0.1763085780832796
1106	98-99	0.25062994697824337
1106	100	0.2949030693481838
1107	1	0.01857710912751287
1107	2	-0.6355059390016464
1107	3	-0.1436813892262805
1107	4	0.007834588026852884
1107	5	0.06338309999323144
1107	6	0.10921517886775689
1107	7	0.11729602220520974
1107	8	0.08514132967400201
1107	9	0.1789791004181751
1107	10-11	0.19215575862023826
1107	12-13	0.16591859695396494
1107	14-15	0.15787080564637535
1107	16-17	0.21128561871058338
1107	18-19	0.21172617110399017
1107	20-21	0.16634883894703023
1107	22-23	0.13742911376236577
1107	24-25	0.15559678198255966
1107	26-27	0.21346116941429472
1107	28-29	0.09485403277692939
1107	30-31	0.19195238454549468
1107	32-33	0.20205954157005124
1107	34-35	0.2282734351267095
1107	36-37	0.2583633027253285
1107	38-39	0.2667145319889954
1107	40-41	0.25516693632112464
1107	42-43	0.2752035862928537
1107	44-45	0.25383509715464925
1107	46-47	0.2706047274146357
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2216	76-77	-0.7362220982716892
2216	78-79	-0.5954256754187526
2216	80-81	-0.5418852222260142
2216	82-83	-0.4742897016623644
2216	84-85	-0.5725219426815258
2216	86-87	-0.4976879247881314
2216	88-89	-0.5569125582674417
2216	90-91	-0.6136416064837569
2216	92-93	-0.5084032182491782
2216	94-95	-0.5244868331300552
2216	96-97	-0.589382590522618
2216	98-99	-0.6235304352297639
2216	100	-0.6422683530223274
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	13.0
13	61.0
14	235.0
15	834.0
16	2003.0
17	4088.0
18	7380.0
19	11117.0
20	16460.0
21	23508.0
22	32297.0
23	38882.0
24	42061.0
25	45864.0
26	55123.0
27	69667.0
28	88991.0
29	111340.0
30	137926.0
31	170281.0
32	212303.0
33	268479.0
34	363928.0
35	502247.0
36	750801.0
37	1275471.0
38	1092131.0
39	377320.0
40	571.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.87577735633852	21.66028631166948	13.736062580397649	24.72787375159436
2	14.95260580377189	20.75681620453525	39.59902615626403	24.69155183542883
3	18.160456205205342	26.709550498535943	29.51505395091009	25.61493934534862
4	12.611378185025291	17.529483737868294	38.94618389964485	30.912954177461565
5	12.960663266702168	38.672386128200976	34.82122122290675	13.545729382190109
6	29.369798631349862	38.90395735491593	18.025219841357817	13.70102417237639
7	25.98744791597239	32.22948052430962	24.02060278908696	17.762468770631028
8	25.87054301201252	34.849064718321024	20.00335357169417	19.27703869797228
9	25.87070086842072	14.976065460596935	21.70204638031748	37.45118729066486
10-11	25.50376364756347	25.910050963064407	27.40624907917095	21.17993631020117
12-13	26.028618665222343	23.645556938455645	27.708465172666845	22.617359223655168
14-15	23.305678543368998	24.68471101221022	25.494919118641484	26.514691325779292
16-17	22.056538924665926	27.163169891277374	26.76114084654034	24.01915033751637
18-19	21.506699075171923	27.396839083282238	28.972263576703483	22.124198264842363
20-21	23.04442555233548	26.614569347321286	28.166289886608176	22.17471521373506
22-23	23.51729130701119	26.076133756098884	27.9134187137339	22.493156223156017
24-25	23.01216888848119	26.17795312313231	28.530110279373417	22.279767709013086
26-27	22.893887415461094	26.531583905549706	27.519399288135887	23.055129390853317
28-29	22.020767238270565	27.002794409217127	28.223769877629714	22.75266847488259
30-31	22.949261091692826	26.522867079291622	28.09167037336899	22.43620145564656
32-33	23.238427722110984	26.804526760519902	27.292802940479017	22.664242576890103
34-35	23.15797883517331	26.635833012064168	28.049578117167417	22.156610035595104
36-37	22.99453080164395	26.204251458944007	27.65423272665023	23.146985012761814
38-39	23.108126026943633	26.20386558772396	28.284974315008427	22.403034070323976
40-41	22.71002083541252	25.52127557135142	28.809382197307272	22.95932139592879
42-43	22.787871038489445	25.752662230157213	27.99528846695218	23.46417826440116
44-45	23.028005129982475	26.51269411076328	27.700388186447363	22.75891257280688
46-47	22.990610700840357	26.37757779514588	27.017781998195527	23.614029505818237
48-49	23.86787599619107	26.061745824359566	27.199907881321256	22.870470298128108
50-51	23.330837155578116	26.14119888620017	26.894919452445183	23.63304450577654
52-53	22.72758684557995	26.58137918793051	26.840088301366826	23.850945665122715
54-55	22.84499236102579	26.547901906468013	26.65941049622506	23.947695236281135
56-57	23.846651431750608	25.996561883208212	26.446777681952977	23.7100090030882
58-59	22.57106144155904	26.429617167385587	27.43481434020496	23.56450705085042
60-61	22.84530837586033	26.96361064258407	26.692807713082022	23.49827326847358
62-63	23.345340359463595	26.251511913598524	26.178810267822687	24.22433745911519
64-65	22.363622587077103	26.420961997999083	26.763326237980113	24.4520891769437
66-67	23.213337323007888	26.03776556709739	26.71091791045823	24.03797919943649
68-69	22.568489159155423	25.98451700059574	27.045628569206016	24.40136527104282
70-71	22.514098278879974	25.743843549117116	27.103529509368578	24.638528662634325
72-73	23.109511655415595	25.767445237858034	27.35667339719619	23.766369709530178
74-75	23.175255761008216	26.258577437781373	26.91362203892312	23.65254476228729
76-77	22.83523699254878	26.275724130987566	26.370510977225376	24.518527899238286
78-79	22.71116968592181	26.366943554255823	26.49320310221209	24.428683657610275
80-81	22.340940262666514	26.780685098102875	27.104275805190277	23.774098834040338
82-83	23.19281607000392	25.680651916705376	28.023073062270836	23.10345895101986
84-85	23.469485928641596	26.18883677290907	26.60792502595786	23.733752272491472
86-87	22.04373436764647	26.858784297333216	27.094220105291832	24.00326122972848
88-89	22.91663980706341	25.6289378919145	27.00365843144653	24.450763869575557
90-91	22.58420394439631	25.755669003001124	27.065214331213628	24.594912721388937
92-93	22.869208947977008	26.021774944990796	26.88160075385064	24.227415353181552
94-95	21.792497838208547	25.944619551013893	27.542091351255593	24.720791259521967
96-97	22.158525080333032	25.947576353764763	27.00197619393625	24.89192237196595
98-99	21.519101018749648	26.63331844479464	26.888446549517575	24.959133986938138
100	22.360116410867377	25.77137822665994	26.189724295686695	25.678781066785987
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	125.0
1	91.0
2	76.5
3	98.0
4	72.5
5	60.5
6	121.5
7	286.5
8	482.0
9	546.0
10	442.0
11	340.5
12	356.0
13	397.0
14	496.5
15	701.0
16	951.5
17	1304.5
18	1824.5
19	2399.5
20	3105.0
21	3947.5
22	4899.0
23	6005.5
24	7151.5
25	8494.0
26	10484.5
27	12884.5
28	16922.0
29	25459.0
30	37895.0
31	50554.0
32	67467.0
33	86459.5
34	103220.0
35	119178.5
36	134709.5
37	162091.5
38	175088.5
39	168535.5
40	160072.0
41	150502.5
42	151319.0
43	162561.5
44	180450.5
45	215467.0
46	249435.0
47	272235.5
48	286215.0
49	278134.0
50	262688.5
51	264773.0
52	266818.5
53	255817.5
54	244374.0
55	212708.0
56	184986.0
57	153052.0
58	118968.5
59	112883.0
60	93399.5
61	63167.5
62	45591.5
63	37125.0
64	26716.0
65	12441.5
66	6450.0
67	4492.5
68	3286.5
69	2459.0
70	1941.5
71	1517.5
72	1092.5
73	839.5
74	624.0
75	435.0
76	290.0
77	161.0
78	98.0
79	65.5
80	38.5
81	13.5
82	4.5
83	4.0
84	3.5
85	2.0
86	1.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.014259695540591548
2	0.0046304546404872925
3	0.001578564081984304
4	0.027133762609219098
5	0.0025958609348186333
6	0.0
7	3.5079201821873425E-4
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.008822419258201167
16-17	0.0031395885630576712
18-19	0.0
20-21	5.261880273281014E-5
22-23	6.401954332491901E-4
24-25	8.769800455468356E-6
26-27	2.630940136640507E-4
28-29	0.0
30-31	0.0
32-33	0.0
34-35	6.665048346155951E-4
36-37	0.0
38-39	0.0
40-41	5.788068300609116E-4
42-43	9.646780501015191E-4
44-45	0.0
46-47	0.0
48-49	7.542028391702787E-4
50-51	8.769800455468356E-6
52-53	0.0
54-55	5.174182268726329E-4
56-57	1.2277720637655698E-4
58-59	2.630940136640507E-4
60-61	9.646780501015193E-5
62-63	0.0
64-65	0.0
66-67	0.0
68-69	0.0017276506897272662
70-71	0.001183923061488228
72-73	0.0
74-75	2.894034150304558E-4
76-77	0.0029992717557701777
78-79	5.788068300609116E-4
80-81	0.003516689982642811
82-83	0.00181534869428195
84-85	0.005568823289222407
86-87	0.008190993625407445
88-89	0.007778813004000433
90-91	0.006305486527481747
92-93	0.00912059247368709
94-95	0.001648722485628051
96-97	0.004797080849141191
98-99	0.002288917918877241
100	0.08182223824951977
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5701384.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.099152112129
#Duplication Level	Percentage of deduplicated	Percentage of total
1	64.58381514218765	14.27247554808825
2	16.631620371579245	7.350894169254264
3	7.0406062040099	4.667742823920419
4	3.598377057219327	3.1808432777714017
5	2.104996924221245	2.3259323611964495
6	1.3452624024458157	1.783749507742691
7	0.901027020930586	1.3938353234878422
8	0.6201919247315404	1.0964572546685099
9	0.4446718073449041	0.8844182919441322
>10	2.104594728116704	8.302701902449677
>50	0.2174605896672541	3.404267366879954
>100	0.28740577384498556	14.205317119639037
>500	0.06248605709497292	9.607740554856555
>1k	0.054149289620551176	21.73454176654474
>5k	0.0028583202732255755	4.2221160039429435
>10k+	4.763867122042626E-4	1.5669667276132642
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	21749	0.38146878021196257	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	16073	0.2819140054414858	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	14067	0.24672956601414675	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	13060	0.22906718789683345	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	12843	0.22526109449916018	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	11513	0.20193342528761438	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	9622	0.16876603996503306	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	9277	0.16271487765075987	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	8541	0.14980573138031048	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	8479	0.1487182761238324	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	8451	0.14822716729832616	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	7973	0.13984323806289842	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	7893	0.13844006999002348	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	7696	0.13498476861056893	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7370	0.12926685871360358	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	7317	0.12833725986532393	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	7258	0.12730242341157866	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	7246	0.12709194820064743	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	7139	0.1252152109031772	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	6844	0.12004102863445087	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	6835	0.11988317222625243	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	6769	0.11872555856613061	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	6733	0.1180941329333369	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	6682	0.11719961328687911	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	6466	0.11341105949011679	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6414	0.11249900024274807	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	6388	0.11204297061906372	No Hit
GTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTTCGCCTTGACCGCGGG	5967	0.10465879863555937	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	5917	0.10378181859001254	No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA	5915	0.10374673938819065	No Hit
ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT	5851	0.10262420492989072	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	5820	0.10208047730165166	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	5806	0.10183492288889855	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.507920182187342E-5	0.0
10-11	0.0	0.0	0.0	3.507920182187342E-5	0.0
12-13	0.0	0.0	0.0	3.507920182187342E-5	0.0
14-15	0.0	0.0	0.0	3.507920182187342E-5	0.0
16-17	0.0	0.0	0.0	3.507920182187342E-5	0.0
18-19	0.0	0.0	0.0	3.507920182187342E-5	0.0
20-21	0.0	0.0	0.0	4.384900227734178E-5	0.0
22-23	0.0	0.0	0.0	9.646780501015192E-5	0.0
24-25	0.0	0.0	0.0	1.6662620865389875E-4	0.0
26-27	0.0	0.0	0.0	4.735692245952912E-4	0.0
28-29	0.0	0.0	0.0	0.0013943982724194687	0.0
30-31	0.0	0.0	0.0	0.006384414731580963	0.0
32-33	0.0	0.0	0.0	0.01825872454828512	0.0
34-35	0.0	0.0	0.0	0.03629820408518353	0.0
36-37	0.0	0.0	0.0	0.06907971818772424	0.0
38-39	0.0	0.0	0.0	0.12134772890231564	0.0
40-41	0.0	0.0	0.0	0.17893900849337635	0.0
42-43	0.0	0.0	0.0	0.2417746287568071	0.0
44-45	0.0	0.0	0.0	0.32036958043871455	0.0
46-47	0.0	0.0	0.0	0.4212222856766006	0.0
48-49	0.0	0.0	0.0	0.5381763445507266	0.0
50-51	0.0	0.0	0.0	0.6665136044160506	0.0
52-53	0.0	0.0	0.0	0.7947631662768198	0.0
54-55	0.0	0.0	0.0	0.9390526931706407	0.0
56-57	0.0	0.0	0.0	1.1180969392694826	0.0
58-59	0.0	0.0	0.0	1.3304664270991045	0.0
60-61	0.0	0.0	0.0	1.556692199648366	0.0
62-63	0.0	0.0	0.0	1.796476083701782	0.0
64-65	0.0	0.0	0.0	2.053159373232885	0.0
66-67	0.0	0.0	0.0	2.3448166269803963	0.0
68-69	0.0	0.0	0.0	2.6719126443684553	0.0
70-71	0.0	0.0	0.0	3.041472035561892	0.0
72-73	0.0	0.0	0.0	3.4299654259386845	0.0
74-75	0.0	0.0	0.0	3.81346003005586	0.0
76-77	0.0	0.0	0.0	4.258001215143551	0.0
78-79	0.0	0.0	0.0	4.739191396334644	0.0
80-81	0.0	0.0	0.0	5.261354085253686	0.0
82-83	0.0	0.0	0.0	5.805906074735538	0.0
84-85	0.0	0.0	0.0	6.343968411880343	0.0
86-87	0.0	0.0	0.0	6.922827860743988	0.0
88	0.0	0.0	0.0	7.388574423333001	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	16890	0.0	37.91639	1
GGTATCA	9930	0.0	37.407387	1
GGGCACC	5685	0.0	36.573433	94
CAACGCA	18025	0.0	33.0875	5
ATCAACG	18570	0.0	32.124607	3
TATCAAC	21115	0.0	30.301933	2
GTACTAG	6400	0.0	28.652609	1
AACGCAG	21785	0.0	28.583847	6
GTGGTAT	2560	0.0	28.101597	1
TAGGCAT	7720	0.0	27.638607	5
ACGCAGA	22055	0.0	26.955402	7
TATTCTC	3155	0.0	26.81333	5
CGCAGAG	22305	0.0	26.610907	8
CTGTGCG	8475	0.0	26.066881	9
TAGTACT	8720	0.0	25.229788	4
TCAACGC	24125	0.0	25.136606	4
CTAGGCA	9215	0.0	24.28264	4
GCAGAGT	24320	0.0	24.197565	9
GTACATG	21965	0.0	23.632921	1
ACTGTGC	9590	0.0	23.473372	8
>>END_MODULE