FastQCFastQC Report
Mon 16 Jan 2017
ERR1146473_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146473_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3081780
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN95860.31105400125901267No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC60820.19735347753570986No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT55420.1798311365509543No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA46950.1523470202285692No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC41270.13391611341497447No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA37060.1202551772027854No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC36750.11924926503514204No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA54250.034.0117261
GGTATCA34250.033.3431361
CAACGCA51950.032.472265
ATCAACG54950.030.8760053
GTACTAG21350.030.5023211
AACGCAG63350.027.6383516
TATCAAC66250.027.453172
TAGGCAT27350.026.9740355
GGGCACC27500.026.4468994
TAGTACT31300.025.5232854
ACGCAGA66150.025.2625477
TCAACGC68550.025.158844
GTACATG113400.025.1348081
ACATGGG110100.024.8864463
TACATGG113000.024.7460542
CGCAGAG67350.024.7431418
CTAGTAC34500.024.657053
CTGTGCG32750.024.5143159
GCATCGT25200.024.4025298
TCTAACG9850.023.8561972
Produced by FastQC (version 0.11.5)