##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146473_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3081780
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.372810518596395	31.0	30.0	34.0	26.0	34.0
2	30.489744563206976	31.0	30.0	34.0	26.0	34.0
3	30.69924524138647	31.0	31.0	34.0	27.0	34.0
4	34.14980692976137	37.0	35.0	37.0	30.0	37.0
5	34.329968070400874	37.0	35.0	37.0	30.0	37.0
6	34.532730110520546	37.0	35.0	37.0	30.0	37.0
7	34.42864480916873	37.0	35.0	37.0	30.0	37.0
8	34.36855583461506	37.0	35.0	37.0	30.0	37.0
9	35.75518661293149	39.0	35.0	39.0	30.0	39.0
10-11	35.679995489619635	39.0	35.0	39.0	29.5	39.0
12-13	35.67914760300865	39.0	35.0	39.0	30.0	39.0
14-15	36.70083312241627	40.0	36.0	41.0	29.5	41.0
16-17	36.51589941527299	40.0	36.0	41.0	28.0	41.0
18-19	36.458937529609514	40.0	36.0	41.0	27.5	41.0
20-21	36.7903450278735	40.0	36.0	41.0	29.5	41.0
22-23	36.515845063567156	40.0	36.0	41.0	28.0	41.0
24-25	36.62621147518642	39.5	36.0	41.0	29.0	41.0
26-27	36.701626008345826	40.0	36.0	41.0	29.5	41.0
28-29	36.52309395868622	39.5	35.5	41.0	28.5	41.0
30-31	36.45881974053956	39.0	35.0	41.0	28.5	41.0
32-33	36.55880481410094	39.0	35.0	41.0	29.5	41.0
34-35	36.602686758950995	40.0	35.0	41.0	29.5	41.0
36-37	36.53279938866499	39.0	35.0	41.0	29.0	41.0
38-39	36.377609368611644	39.0	35.0	41.0	29.0	41.0
40-41	36.346227504883544	39.0	35.0	41.0	29.0	41.0
42-43	36.189707247110434	39.0	35.0	41.0	28.0	41.0
44-45	36.03154783923577	39.0	35.0	41.0	28.0	41.0
46-47	35.913542822654435	39.0	34.5	41.0	27.5	41.0
48-49	35.80142855752195	39.0	34.0	40.5	27.0	41.0
50-51	35.60873180434684	38.0	34.0	40.0	27.0	41.0
52-53	35.44525014764194	38.0	34.0	40.0	27.0	41.0
54-55	35.25987221670593	38.0	33.5	40.0	26.0	41.0
56-57	35.14179694851677	38.0	33.0	40.0	26.0	41.0
58-59	34.91421045629474	37.0	33.0	40.0	26.0	41.0
60-61	34.6620477451343	37.0	33.0	40.0	26.0	41.0
62-63	34.39680217277028	36.0	33.0	40.0	26.0	41.0
64-65	34.1152798058265	36.0	32.5	39.0	26.0	41.0
66-67	33.81695108021987	35.0	32.0	39.0	25.5	41.0
68-69	33.53035080375627	35.0	32.0	38.5	25.0	40.0
70-71	33.41822761520939	35.0	32.0	38.0	25.0	40.0
72-73	33.18648427207653	35.0	32.0	37.0	25.0	40.0
74-75	32.907655640571356	35.0	32.0	37.0	25.0	39.0
76-77	32.658089805242426	35.0	31.0	36.0	25.0	39.0
78-79	32.425122494143	35.0	31.0	36.0	24.0	39.0
80-81	32.169528811271405	35.0	31.0	35.5	24.0	37.5
82-83	31.945445164807353	35.0	31.0	35.0	24.0	37.0
84-85	31.752950405285258	34.5	31.0	35.0	24.0	37.0
86-87	31.582699121936024	34.5	31.0	35.0	24.0	36.0
88-89	31.560251218451675	35.0	31.0	35.0	24.0	36.0
90-91	31.489777336474376	35.0	31.0	35.0	23.5	36.0
92-93	31.36010422548008	35.0	31.0	35.0	23.0	35.5
94-95	31.323398165994977	35.0	31.0	35.0	23.0	35.0
96-97	31.237042391085673	34.0	31.0	35.0	23.0	35.0
98-99	31.16161828553628	34.0	31.0	35.0	23.0	35.0
100	30.679600750215783	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.77198725517054
1101	2	0.9139107887469606
1101	3	0.8625819417090668
1101	4	0.9489661108269019
1101	5	0.7747915317110596
1101	6	0.5057810370057467
1101	7	0.6006154555207956
1101	8	0.6006223796619423
1101	9	0.8639018094158004
1101	10-11	0.9187549521293477
1101	12-13	0.7836395751925807
1101	14-15	0.971179765557352
1101	16-17	1.088123819293152
1101	18-19	1.2797518171235964
1101	20-21	0.8965869943055296
1101	22-23	1.1784802893829038
1101	24-25	1.0412126606381094
1101	26-27	0.9749136568551933
1101	28-29	1.1546578453769598
1101	30-31	1.1459430097074232
1101	32-33	0.9983664456504542
1101	34-35	0.9453757078173837
1101	36-37	0.8873941758236654
1101	38-39	1.0199372403687619
1101	40-41	0.9011709697886374
1101	42-43	0.9511982351484107
1101	44-45	0.9760839233732597
1101	46-47	1.0159331843066823
1101	48-49	0.9266202410200606
1101	50-51	0.9314913442001611
1101	52-53	0.9488016657175251
1101	54-55	0.9670363687368919
1101	56-57	0.8475516154916747
1101	58-59	0.8367550670370605
1101	60-61	0.8492287413034205
1101	62-63	0.7619123494627047
1101	64-65	0.7581235544330127
1101	66-67	0.6994668626243694
1101	68-69	0.7588679327676431
1101	70-71	0.6710615575233732
1101	72-73	0.6587187104185332
1101	74-75	0.6566859346317884
1101	76-77	0.7401097890082724
1101	78-79	0.6649664610204127
1101	80-81	0.6682685529855164
1101	82-83	0.6706687673740497
1101	84-85	0.7049482961302154
1101	86-87	0.6593727036806918
1101	88-89	0.6655304130658202
1101	90-91	0.7272141004465134
1101	92-93	0.669206853394769
1101	94-95	0.7300719152834425
1101	96-97	0.6879294584233442
1101	98-99	0.6943511202984318
1101	100	0.8308238981994158
1102	1	0.7648652696993139
1102	2	0.9048443259657581
1102	3	0.82840833094102
1102	4	0.8698185620848733
1102	5	0.7760770965887076
1102	6	0.6050422920932874
1102	7	0.6018994381647502
1102	8	0.6340974342533983
1102	9	0.6674220508435695
1102	10-11	0.7267364333145636
1102	12-13	0.7396995390367778
1102	14-15	0.9730283419250085
1102	16-17	1.1244885600239414
1102	18-19	1.0768996562934134
1102	20-21	0.8516517771061558
1102	22-23	1.1715688288950474
1102	24-25	0.9598956936957279
1102	26-27	0.8884346964319505
1102	28-29	1.031041223062644
1102	30-31	1.100852425949327
1102	32-33	0.9352940396478786
1102	34-35	0.8405956215997179
1102	36-37	0.8223754465717974
1102	38-39	0.8897289885277928
1102	40-41	0.7917201297016518
1102	42-43	0.8444615851133648
1102	44-45	0.9394165804481887
1102	46-47	0.8720720137104294
1102	48-49	0.794000972683591
1102	50-51	0.9152182024940814
1102	52-53	0.8913579242341143
1102	54-55	0.887410809615055
1102	56-57	0.833951657614243
1102	58-59	0.7887942674042705
1102	60-61	0.841008949353089
1102	62-63	0.8210414774884356
1102	64-65	0.7394228267285783
1102	66-67	0.7371652505567781
1102	68-69	0.8136901767231208
1102	70-71	0.6732075059239264
1102	72-73	0.6244361892311048
1102	74-75	0.6655851641015573
1102	76-77	0.7376186939074074
1102	78-79	0.6423016665464161
1102	80-81	0.6725672182307534
1102	82-83	0.6247062409968969
1102	84-85	0.5659924455486269
1102	86-87	0.7210635559614254
1102	88-89	0.703865694011629
1102	90-91	0.6710829536663887
1102	92-93	0.6730438580083913
1102	94-95	0.7044501897380506
1102	96-97	0.741670773059127
1102	98-99	0.7254149386397231
1102	100	0.7223066638946918
1103	1	0.5592254110363584
1103	2	0.8213248923822789
1103	3	0.7366930865664507
1103	4	0.7257480486112087
1103	5	0.7291838224987401
1103	6	0.5537369397162593
1103	7	0.5454719750315036
1103	8	0.5590081620652967
1103	9	0.6150308812263674
1103	10-11	0.7670472520224152
1103	12-13	0.6771774860162196
1103	14-15	0.8941998009784271
1103	16-17	1.0471383139623143
1103	18-19	1.1036923473641664
1103	20-21	0.8556462083634742
1103	22-23	1.0643805912922417
1103	24-25	0.9596546659419332
1103	26-27	0.8453338698268666
1103	28-29	1.048532384727963
1103	30-31	1.02834710222335
1103	32-33	0.7858146014961918
1103	34-35	0.8407894540467922
1103	36-37	0.7994566078442986
1103	38-39	0.874319081354912
1103	40-41	0.817251446530058
1103	42-43	0.8921986731321567
1103	44-45	0.9741168158914206
1103	46-47	0.8673626071590022
1103	48-49	0.7973077009002836
1103	50-51	0.8682396863526805
1103	52-53	0.866577179711129
1103	54-55	0.8665181388966019
1103	56-57	0.7891025668295484
1103	58-59	0.7786865805917245
1103	60-61	0.8516793594436649
1103	62-63	0.7195441223767318
1103	64-65	0.7562811767251532
1103	66-67	0.7511022609298621
1103	68-69	0.7856314720918576
1103	70-71	0.6819467456324659
1103	72-73	0.7033341031595199
1103	74-75	0.6060787112435833
1103	76-77	0.591897927270054
1103	78-79	0.6028115085347068
1103	80-81	0.6611760011797756
1103	82-83	0.6064309695252454
1103	84-85	0.5774239422871936
1103	86-87	0.6728117420964175
1103	88-89	0.6292626613890668
1103	90-91	0.6598775261324086
1103	92-93	0.672570192295801
1103	94-95	0.6462781912229794
1103	96-97	0.6462188180547912
1103	98-99	0.6293975721543106
1103	100	0.6904963768006773
1104	1	0.5098887595555581
1104	2	0.7285992868680218
1104	3	0.7134426422592774
1104	4	0.7722015146844541
1104	5	0.7467971585405166
1104	6	0.3720900849144897
1104	7	0.4475031354505745
1104	8	0.49689417903205424
1104	9	0.568073851697271
1104	10-11	0.6094433190429456
1104	12-13	0.4711689537246002
1104	14-15	0.6310057487722034
1104	16-17	0.7769583259331085
1104	18-19	0.8202551974912851
1104	20-21	0.6832732627874307
1104	22-23	0.9055005847275268
1104	24-25	0.770254554080779
1104	26-27	0.6930030782045336
1104	28-29	0.8654416164517116
1104	30-31	0.8110230174094255
1104	32-33	0.6416190307615324
1104	34-35	0.5092483760330353
1104	36-37	0.5934878570656892
1104	38-39	0.6649483872329682
1104	40-41	0.5753999461671171
1104	42-43	0.5764430396035678
1104	44-45	0.6293853091389181
1104	46-47	0.609167473167112
1104	48-49	0.6448904277279226
1104	50-51	0.7305308886918809
1104	52-53	0.6888996966911805
1104	54-55	0.6121772139135899
1104	56-57	0.5908112758631887
1104	58-59	0.602057648051229
1104	60-61	0.6112374645580303
1104	62-63	0.47321167354895977
1104	64-65	0.5408046662307342
1104	66-67	0.5711211721459719
1104	68-69	0.5047584892302268
1104	70-71	0.4449124646525533
1104	72-73	0.5132643832664741
1104	74-75	0.43551997343458027
1104	76-77	0.4489772697352876
1104	78-79	0.4722379423145071
1104	80-81	0.630578631733961
1104	82-83	0.4935490544112433
1104	84-85	0.48070623895341313
1104	86-87	0.44490376056747394
1104	88-89	0.514400231203215
1104	90-91	0.4894243177085009
1104	92-93	0.5210317468242138
1104	94-95	0.48893707457933644
1104	96-97	0.44657718884047526
1104	98-99	0.5161089310264302
1104	100	0.584288844988933
1105	1	0.36280723677558413
1105	2	0.4672237947484845
1105	3	0.42329313344142605
1105	4	0.5866796593434884
1105	5	0.48125449634062534
1105	6	0.09307008092612534
1105	7	0.14707432362899198
1105	8	0.13390554545092215
1105	9	0.2487672951186113
1105	10-11	0.35730064508324944
1105	12-13	0.2401756461165121
1105	14-15	0.4155920299121121
1105	16-17	0.5948001258578302
1105	18-19	0.505576668057401
1105	20-21	0.3558530233797015
1105	22-23	0.6126953709695186
1105	24-25	0.562227712528383
1105	26-27	0.31955580250759397
1105	28-29	0.5870849803440592
1105	30-31	0.6638689085680411
1105	32-33	0.4517015954736152
1105	34-35	0.3404823042871712
1105	36-37	0.20624491319323113
1105	38-39	0.3797982326568672
1105	40-41	0.28486446418902034
1105	42-43	0.39958570510204794
1105	44-45	0.3323937479772425
1105	46-47	0.2747006734088231
1105	48-49	0.3419767244373233
1105	50-51	0.3739149032740343
1105	52-53	0.44458012508810896
1105	54-55	0.4253677076134821
1105	56-57	0.417476341055135
1105	58-59	0.30348605154399877
1105	60-61	0.3143846544444955
1105	62-63	0.3084453300133845
1105	64-65	0.294835673020458
1105	66-67	0.41178935561793395
1105	68-69	0.4969467687116449
1105	70-71	0.3709267807713772
1105	72-73	0.37629766371793494
1105	74-75	0.30904054387340096
1105	76-77	0.31720653104927266
1105	78-79	0.3071827792701143
1105	80-81	0.43749161225637323
1105	82-83	0.3495259603612233
1105	84-85	0.26972357701466265
1105	86-87	0.33329987790898485
1105	88-89	0.4281159774005978
1105	90-91	0.4141648987758124
1105	92-93	0.45342004256662705
1105	94-95	0.37915046342626013
1105	96-97	0.3371142943411378
1105	98-99	0.3866391704869869
1105	100	0.4725283439440986
1106	1	0.48143350825835896
1106	2	0.5447092310843189
1106	3	0.4608630760844399
1106	4	0.6122300828666383
1106	5	0.5024292133394823
1106	6	0.28905532154885805
1106	7	0.3466190584246931
1106	8	0.30761343712502054
1106	9	0.5500197128277762
1106	10-11	0.4813281806117047
1106	12-13	0.3701912509743721
1106	14-15	0.5617472121340583
1106	16-17	0.715880483047286
1106	18-19	0.6734351283433
1106	20-21	0.38345395653976055
1106	22-23	0.7564324160670424
1106	24-25	0.5362694333423406
1106	26-27	0.42509324111892255
1106	28-29	0.6536538217746966
1106	30-31	0.6297171917727269
1106	32-33	0.5562022869932335
1106	34-35	0.5186912423739827
1106	36-37	0.3919407385023277
1106	38-39	0.5277232667109502
1106	40-41	0.5228912377411277
1106	42-43	0.4451640399227941
1106	44-45	0.42614303688630883
1106	46-47	0.39221608777259576
1106	48-49	0.40933104865302994
1106	50-51	0.4691876647075901
1106	52-53	0.4576252852336111
1106	54-55	0.45066688770607044
1106	56-57	0.4709470180136961
1106	58-59	0.46415963862589393
1106	60-61	0.4641445692701538
1106	62-63	0.37720743160675596
1106	64-65	0.3248145568816909
1106	66-67	0.38066149494478907
1106	68-69	0.379280691793781
1106	70-71	0.3476111190262401
1106	72-73	0.30992246865720574
1106	74-75	0.3157186088710233
1106	76-77	0.32155140362699797
1106	78-79	0.3869451887854183
1106	80-81	0.44048895577723357
1106	82-83	0.47145931352681814
1106	84-85	0.4278222268552234
1106	86-87	0.43285054150686975
1106	88-89	0.4015156131132791
1106	90-91	0.34965074219489267
1106	92-93	0.4910724456625495
1106	94-95	0.48552899865722665
1106	96-97	0.4135154696635439
1106	98-99	0.49900011763401153
1106	100	0.43413170916562294
1107	1	0.4428545259280803
1107	2	0.512357430898323
1107	3	0.48145242110901876
1107	4	0.5528598158279792
1107	5	0.5274718598121808
1107	6	0.3577860243521016
1107	7	0.3326575863906811
1107	8	0.3707132112714291
1107	9	0.49251579360364417
1107	10-11	0.4582268122046358
1107	12-13	0.4263915963975222
1107	14-15	0.6098465877152961
1107	16-17	0.6789914170183309
1107	18-19	0.7742992176218948
1107	20-21	0.493097639281892
1107	22-23	0.7590013014131287
1107	24-25	0.4660831705550379
1107	26-27	0.41886074477243085
1107	28-29	0.5015220295600074
1107	30-31	0.48693361265753765
1107	32-33	0.4509805519062198
1107	34-35	0.40386727532203537
1107	36-37	0.42827659608170165
1107	38-39	0.4902964034188031
1107	40-41	0.32948669409824305
1107	42-43	0.4429841541781414
1107	44-45	0.38272069066603365
1107	46-47	0.4031877896458482
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2214	4	-0.7012144445584738
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2214	96-97	-0.9805846050190539
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2214	100	-1.125195151675804
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2215	3	-3.8435487356365563
2215	4	-2.153703427599307
2215	5	-1.6402181847552626
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2215	8	-1.338978963453897
2215	9	-1.8817535142259914
2215	10-11	-1.5398657579967292
2215	12-13	-1.4663223687953533
2215	14-15	-3.651194679728647
2215	16-17	-3.3985959172724094
2215	18-19	-2.3701264036377765
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2215	24-25	-5.552938179039657
2215	26-27	-3.716533203483941
2215	28-29	-1.9243308411332976
2215	30-31	-4.046448384016273
2215	32-33	-4.087069611565944
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2215	100	-3.1836941824378613
2216	1	-0.5610280302031789
2216	2	-0.5853420509669611
2216	3	-0.746316411095048
2216	4	-0.6983340196974623
2216	5	-0.8210579661476913
2216	6	-0.6750472425537097
2216	7	-0.9169706851650474
2216	8	-0.7190532295416432
2216	9	-0.9365443897363264
2216	10-11	-0.9649282296447055
2216	12-13	-1.0620138772307541
2216	14-15	-1.5448168904300488
2216	16-17	-1.220222081055276
2216	18-19	-3.369026410118238
2216	20-21	-1.5357255306397235
2216	22-23	-0.9763880967534746
2216	24-25	-1.1430126179397107
2216	26-27	-1.0704965024708244
2216	28-29	-1.0656282295073538
2216	30-31	-1.1946930646375336
2216	32-33	-1.1284130976221505
2216	34-35	-1.3753600396772967
2216	36-37	-1.2810849025233182
2216	38-39	-1.4444818614941752
2216	40-41	-1.4245959417460483
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2216	50-51	-1.3801456686257438
2216	52-53	-1.3272977916894604
2216	54-55	-1.1908715738323892
2216	56-57	-1.2804375973709128
2216	58-59	-1.3696865152202662
2216	60-61	-1.4083682512426634
2216	62-63	-1.4456643632650383
2216	64-65	-1.3785700585029232
2216	66-67	-1.3449795306962358
2216	68-69	-1.490052641539549
2216	70-71	-1.469414521999397
2216	72-73	-1.478077531342791
2216	74-75	-1.4594608783084624
2216	76-77	-1.3558844938088974
2216	78-79	-1.504131734291505
2216	80-81	-1.509152069863795
2216	82-83	-1.6432073531398501
2216	84-85	-1.4825367475037545
2216	86-87	-1.4620212533649237
2216	88-89	-1.300330540732869
2216	90-91	-1.4855287449845918
2216	92-93	-1.3473890927989878
2216	94-95	-1.315188950060719
2216	96-97	-1.2690486329005566
2216	98-99	-1.2699229592890688
2216	100	-1.2926375216036092
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	12951.0
1	2157.0
2	359.0
3	327.0
4	310.0
5	168.0
6	150.0
7	182.0
8	355.0
9	610.0
10	1237.0
11	2269.0
12	4029.0
13	5716.0
14	7648.0
15	9186.0
16	10941.0
17	13004.0
18	15043.0
19	16881.0
20	19092.0
21	21802.0
22	24722.0
23	28820.0
24	32383.0
25	36406.0
26	41213.0
27	47207.0
28	55628.0
29	66229.0
30	79105.0
31	94095.0
32	115471.0
33	146794.0
34	196999.0
35	265728.0
36	390363.0
37	596795.0
38	554462.0
39	164797.0
40	146.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.07402991904478	19.830078286976196	12.834665104620285	24.261226689358733
2	15.502188815041054	19.696466929824275	40.52665204197049	24.27469221316418
3	19.077976651057448	25.53270094304216	28.75465024030114	26.63467216559926
4	12.503268378721144	16.589670945172745	38.71439423330845	32.192666442797666
5	13.68810846168111	37.756262362337864	34.70867007073319	13.846959105247839
6	31.093004235469678	37.186008470939356	18.055923331085374	13.665063962505592
7	27.715922863863668	31.402831612717208	22.87498581192946	18.006259711489665
8	25.92868875879534	35.297870591566124	20.113750673553767	18.659689976084763
9	26.081423525251484	15.704651001956021	20.89306988573121	37.32085558706128
10-11	25.535016511551234	25.811136173845433	27.744185883378186	20.909661431225143
12-13	25.863023844033773	23.466418272622793	27.591265643237527	23.079292240105907
14-15	23.152549849058907	24.876717030390168	24.97730092593952	26.993432194611405
16-17	22.739331960732592	27.52431537582001	26.082187365058452	23.65416529838895
18-19	22.436815457629642	27.636030021701117	27.444613863495515	22.482540657173725
20-21	23.70652269130652	26.893228732930808	26.735124238127035	22.66512433763564
22-23	23.82826233148026	26.625173093718796	26.553408095470115	22.993156479330832
24-25	23.450197250885115	26.796611043178565	26.876004035062763	22.877187670873557
26-27	23.212450950652197	26.98602970196915	26.3078195179709	23.493699829407756
28-29	22.769484002321903	27.289583017664974	26.75741713940127	23.18351584061185
30-31	23.288452357437837	26.9553676530434	26.656686713020495	23.09949327649827
32-33	23.404195627625775	27.141205298940058	26.049865180968716	23.40473389246545
34-35	23.59459776902673	27.05595051271929	26.525841508892245	22.823610209361735
36-37	23.4395571028291	26.736599630897267	26.35782145524242	23.466021811031215
38-39	23.373321123769315	26.860632982039185	26.71249984297561	23.05354605121589
40-41	23.252509955882335	26.338968079668597	27.170320351897143	23.238201612551926
42-43	23.166774260437613	26.46084035384728	26.686485455589875	23.685899930125228
44-45	23.337558163478082	26.98348106641936	26.59946097519827	23.079499794904294
46-47	23.317072756861144	26.8259700626569	26.266574252553543	23.590382927928413
48-49	23.63039841531652	26.608465477284117	26.434520889009523	23.326615218389847
50-51	23.399200419986695	26.59892347413046	26.48461152699031	23.517264578892537
52-53	23.16519695371554	26.886775062958566	26.360774536387098	23.58725344693879
54-55	23.062723463513983	26.776483954349	26.489109630653434	23.67168295148358
56-57	23.64841454558267	26.57419895353549	26.24325569347033	23.53413080741151
58-59	23.03668457426018	26.69243398192623	26.755142098157453	23.515739345656144
60-61	23.031314575341785	27.074730084450767	26.442899004247007	23.451056335960445
62-63	23.261981102176748	26.665947886713536	26.228465349885717	23.843605661224
64-65	22.784349850890603	26.770964670346782	26.440755992230425	24.003929486532193
66-67	23.182022982190613	26.5839202967372	26.387383122533294	23.846673598538892
68-69	22.932512084191632	26.57655768799331	26.5782708898452	23.912659337969856
70-71	22.808127141439975	26.308918096767513	26.69437117460904	24.188583587183473
72-73	23.1028925844996	26.438290192920537	26.888395696084327	23.570421526495537
74-75	23.17562279980665	26.659830185327266	26.65611068704843	23.508436327817655
76-77	23.000757987124167	26.545191903516574	26.452527284355764	24.001522825003494
78-79	22.984824568645795	26.49630706042793	26.493745351804176	24.025123019122095
80-81	22.72651716819304	26.70014398924428	26.86199527300292	23.711343569559755
82-83	23.168054704825416	26.180245679083836	27.46283045598501	23.188869160105735
84-85	23.23196746129732	26.506507218496893	26.653803838554158	23.607721481651627
86-87	22.504124956860387	26.81569694384184	26.97454235192988	23.70563574736789
88-89	23.042080086397895	26.041328764693557	26.935301504136532	23.981289644772012
90-91	22.83138749757389	26.112781746413916	27.023013204098405	24.03281755191379
92-93	22.95224085605449	26.218129042779424	27.063673499120444	23.765956602045634
94-95	22.317330386566287	26.107256742001194	27.48181856217129	24.09359430926123
96-97	22.55988709917426	25.949733735766195	27.206201670445285	24.28417749461426
98-99	22.131176990257575	26.399436962154898	27.130090123787937	24.339295923799586
100	22.632024985161863	25.876500095639965	26.694410459173547	24.797064460024625
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	10691.0
1	6280.5
2	1794.0
3	1340.5
4	706.5
5	397.0
6	308.5
7	284.0
8	286.5
9	268.5
10	256.5
11	230.0
12	224.5
13	269.0
14	299.5
15	336.5
16	381.0
17	439.5
18	585.5
19	740.0
20	897.5
21	1174.5
22	1677.5
23	2441.0
24	3392.5
25	4720.5
26	6625.0
27	8989.0
28	12000.0
29	16926.0
30	23986.5
31	31283.0
32	39680.5
33	48036.5
34	55011.5
35	62869.5
36	71516.0
37	83782.5
38	90610.5
39	89526.5
40	88080.0
41	86605.0
42	88069.5
43	92906.5
44	99952.0
45	111868.0
46	123935.5
47	132050.5
48	136525.5
49	135198.0
50	131128.0
51	132048.0
52	131862.5
53	127400.0
54	122046.0
55	110329.5
56	99557.0
57	86549.0
58	73190.0
59	66576.5
60	56009.0
61	43517.0
62	34439.5
63	27474.0
64	20177.5
65	12780.5
66	8978.0
67	6774.0
68	5034.5
69	3792.5
70	2873.5
71	2135.5
72	1573.5
73	1245.5
74	958.0
75	711.0
76	523.0
77	327.5
78	200.5
79	121.5
80	82.0
81	57.5
82	41.5
83	33.0
84	24.0
85	21.0
86	15.0
87	7.5
88	7.5
89	7.5
90	4.5
91	5.0
92	5.0
93	2.0
94	1.0
95	4.5
96	4.5
97	1.5
98	1.0
99	2.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	1.0085405187910883
2	0.45136252425546275
3	0.4868290403597921
4	0.4708317920163023
5	0.4377015880432737
6	0.496336532783002
7	0.5134694884125408
8	0.5176229322015199
9	0.5313812147525131
10-11	0.5452855168117128
12-13	0.5437604241704469
14-15	0.5411482974125343
16-17	0.5449934777953002
18-19	0.5428843071212092
20-21	0.5424949217659925
22-23	0.5036699569729183
24-25	0.5402072828040937
26-27	0.518855985826373
28-29	0.5254917612548592
30-31	0.528963780672209
32-33	0.531332541583111
34-35	0.5435170583234364
36-37	0.5620615358656361
38-39	0.5506395654459436
40-41	0.5559936140801744
42-43	0.5520024141892024
44-45	0.5632134675414857
46-47	0.5617857212390243
48-49	0.5333281415285971
50-51	0.5490657996352758
52-53	0.5408887071757231
54-55	0.550396199598933
56-57	0.5387146389424293
58-59	0.5446852143890868
60-61	0.5374166877583734
62-63	0.5321762098527474
64-65	0.5434683851540344
66-67	0.5422515559189819
68-69	0.5627429602372656
70-71	0.5456424533873281
72-73	0.5497796727865065
74-75	0.546778160673377
76-77	0.532954980563181
78-79	0.5652252918767725
80-81	0.5393149413650553
82-83	0.5387308633322301
84-85	0.5476056045532127
86-87	0.8531919864493895
88-89	1.118947491384849
90-91	1.1098618330964574
92-93	1.110299891621076
94-95	1.1153456768490937
96-97	1.1081095989979817
98-99	1.104150847886611
100	1.09972158947102
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	3081780.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.03374048462418
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.70256085159298	38.590809442723526
2	12.107702848322099	11.873719186591416
3	4.025475434843042	5.921523533979702
4	1.7444867410188887	3.4215484055195193
5	0.898960061437303	2.2039687179279266
6	0.5254921041349114	1.5460106076522213
7	0.35179915537409573	1.2075019941126375
8	0.25727765391010066	1.0092228571456667
9	0.17374466473162148	0.7667415720934527
>10	0.9710869328588346	9.012820573906556
>50	0.1084487039236966	3.759276652130251
>100	0.11457818305448053	11.721227164153621
>500	0.013823068139658579	4.533584540318
>1k	0.004365179412451673	3.7441945714881255
>5k	1.9841724602053055E-4	0.687850180257448
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	9586	0.31105400125901267	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	6082	0.19735347753570986	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	5542	0.1798311365509543	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	4695	0.1523470202285692	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	4127	0.13391611341497447	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	3706	0.1202551772027854	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	3675	0.11924926503514204	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.244877960139919E-5
2	0.0	0.0	0.0	0.0	3.244877960139919E-5
3	0.0	0.0	0.0	6.489755920279838E-5	3.244877960139919E-5
4	0.0	0.0	0.0	6.489755920279838E-5	3.244877960139919E-5
5	0.0	3.244877960139919E-5	0.0	9.734633880419757E-5	3.244877960139919E-5
6	0.0	3.244877960139919E-5	0.0	9.734633880419757E-5	3.244877960139919E-5
7	0.0	3.244877960139919E-5	0.0	1.2979511840559676E-4	3.244877960139919E-5
8	0.0	3.244877960139919E-5	0.0	2.2714145720979434E-4	3.244877960139919E-5
9	0.0	3.244877960139919E-5	0.0	2.595902368111935E-4	3.244877960139919E-5
10-11	0.0	3.244877960139919E-5	0.0	3.893853552167903E-4	3.244877960139919E-5
12-13	0.0	6.489755920279838E-5	0.0	4.705073042202883E-4	1.2979511840559676E-4
14-15	0.0	6.489755920279838E-5	0.0	5.516292532237862E-4	1.2979511840559676E-4
16-17	0.0	6.489755920279838E-5	0.0	6.651999818286835E-4	1.6224389800699595E-4
18-19	0.0	6.489755920279838E-5	0.0	7.138731512307822E-4	1.9469267760839515E-4
20-21	0.0	6.489755920279838E-5	0.0	7.138731512307822E-4	2.2714145720979434E-4
22-23	0.0	6.489755920279838E-5	0.0	7.787707104335806E-4	2.2714145720979434E-4
24-25	0.0	6.489755920279838E-5	0.0	9.24790218639877E-4	2.2714145720979434E-4
26-27	0.0	6.489755920279838E-5	0.0	0.001119482896248272	2.2714145720979434E-4
28-29	0.0	6.489755920279838E-5	0.0	0.0017360097086748568	2.758146266118931E-4
30-31	0.0	6.489755920279838E-5	0.0	0.00520802912602457	2.920390164125927E-4
32-33	0.0	6.489755920279838E-5	0.0	0.01403409717760515	3.569365756153911E-4
34-35	0.0	6.489755920279838E-5	0.0	0.026916262679360627	3.893853552167903E-4
36-37	0.0	6.489755920279838E-5	0.0	0.0523074327174555	3.893853552167903E-4
38-39	0.0	6.489755920279838E-5	0.0	0.09419880718286186	3.893853552167903E-4
40-41	0.0	6.489755920279838E-5	0.0	0.14243391806034172	3.893853552167903E-4
42-43	0.0	6.489755920279838E-5	0.0	0.19378411177955596	3.893853552167903E-4
44-45	0.0	6.489755920279838E-5	0.0	0.2592333002355781	4.380585246188891E-4
46-47	0.0	6.489755920279838E-5	0.0	0.3409393272719013	4.867316940209879E-4
48-49	0.0	6.489755920279838E-5	0.0	0.43916178312533666	5.354048634230867E-4
50-51	0.0	6.489755920279838E-5	0.0	0.5477353996716183	5.840780328251854E-4
52-53	0.0	6.489755920279838E-5	0.0	0.6555789186768686	6.81424371629383E-4
54-55	0.0	8.112194900349798E-5	0.0	0.7783813250783638	7.300975410314818E-4
56-57	0.0	9.734633880419757E-5	0.0	0.9313773208989611	8.274438798356793E-4
58-59	0.0	9.734633880419757E-5	0.0	1.1120034525501494	8.43668269636379E-4
60-61	0.0	9.734633880419757E-5	0.0	1.3038893107230236	8.598926594370786E-4
62-63	0.0	9.734633880419757E-5	0.0	1.503222163814419	8.761170492377782E-4
64-65	0.0	9.734633880419757E-5	0.0	1.7160699336097969	9.24790218639877E-4
66-67	0.0	9.734633880419757E-5	0.0	1.9618694390903957	9.410146084405766E-4
68-69	0.0	9.734633880419757E-5	0.0	2.2440764752837645	9.734633880419758E-4
70-71	0.0	9.734633880419757E-5	0.0	2.558278008164113	0.0010221365574440745
72-73	0.0	9.734633880419757E-5	0.0	2.8875844479489126	0.001087034116646873
74-75	0.0	9.734633880419757E-5	0.0	3.21869179500159	0.001119482896248272
76-77	0.0	9.734633880419757E-5	0.0	3.5849411703625824	0.0011519316758496713
78-79	0.0	9.734633880419757E-5	0.0	3.998517090772216	0.0011681560656503708
80-81	0.0	9.734633880419757E-5	0.0	4.453595000292039	0.0011681560656503708
82-83	0.0	9.734633880419757E-5	0.0	4.921717319211624	0.0012330536248531692
84-85	0.0	9.734633880419757E-5	0.0	5.389612496674	0.0012655024044545685
86-87	0.0	9.734633880419757E-5	0.0	5.893963878018548	0.001330399963657367
88	0.0	9.734633880419757E-5	0.0	6.2984054669703875	0.0013952975228601653
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	5425	0.0	34.011726	1
GGTATCA	3425	0.0	33.343136	1
CAACGCA	5195	0.0	32.47226	5
ATCAACG	5495	0.0	30.876005	3
GTACTAG	2135	0.0	30.502321	1
AACGCAG	6335	0.0	27.638351	6
TATCAAC	6625	0.0	27.45317	2
TAGGCAT	2735	0.0	26.974035	5
GGGCACC	2750	0.0	26.44689	94
TAGTACT	3130	0.0	25.523285	4
ACGCAGA	6615	0.0	25.262547	7
TCAACGC	6855	0.0	25.15884	4
GTACATG	11340	0.0	25.134808	1
ACATGGG	11010	0.0	24.886446	3
TACATGG	11300	0.0	24.746054	2
CGCAGAG	6735	0.0	24.743141	8
CTAGTAC	3450	0.0	24.65705	3
CTGTGCG	3275	0.0	24.514315	9
GCATCGT	2520	0.0	24.402529	8
TCTAACG	985	0.0	23.856197	2
>>END_MODULE