FastQCFastQC Report
Mon 16 Jan 2017
ERR1146473_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146473_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3081780
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC70360.22830961327544472No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT54470.1767485024888214No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51530.16720856128601003No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA50570.16409347844427571No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47800.15510516649468814No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC45660.1481611276599887No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC42680.13849139133877175No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA41940.13609018164826822No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC32300.1048095581125194No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51900.046.6559031
GTATCAA91050.039.2980841
CAACGCA109100.031.964115
ATCAACG109500.031.8985483
GTACTAG22950.031.3455181
TATCAAC119750.029.6009852
AACGCAG122450.029.3226996
TCAACGC124550.028.1195764
TAGGCAT29050.026.8562535
ACGCAGA131650.026.3811027
CGCAGAG132750.026.0558728
GTACATG116400.024.2362251
GCATCGT26800.024.1995148
GTGGTAT20750.024.0189741
TACATGG115550.023.8417422
GCAGAGT146600.023.5347189
ACATGGG114500.023.4437373
CTAGGCA32950.023.398324
CATGGGG91800.021.9178184
GGGCACC24900.021.91334594