FastQCFastQC Report
Mon 16 Jan 2017
ERR1146472_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1146472_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5876088
Sequences flagged as poor quality0
Sequence length100
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC253850.43200510271459514No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT214670.36532808902793834No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN181500.308878968456565No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA180550.30726224658310086No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC159030.2706392416178927No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA153890.26189192537620265No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC147320.25071101726182454No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC131260.22337990853778908No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG107140.18233219107678442No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT103470.1760865392077178No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC94950.16158709672149227No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT93270.15872805172420834No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT93070.1583876892245317No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC87300.1485682311088602No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT84210.14330963048885584No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG82740.14080796611623245No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT82360.14016127736684678No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC78250.1331668279984915No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC77260.1314820336250921No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC76800.13069919987583578No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA74680.12709135737926322No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT74190.1262574692550554No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT73720.12545761738081526No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA73180.1245386386316883No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG71810.1222071555089032No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA70780.12045428863556842No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG70010.1191438930118133No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG68510.1165911742642384No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT67310.11454899926617845No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA67200.1143617998913563No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA66860.11378318364190598No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC63750.10849054677193398No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA63700.10840545614701483No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC63310.10774174927264533No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT62600.10653346239879322No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA62070.10563150177465008No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC61340.10438917865083028No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA60850.10355529052662248No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG60240.10251718490260867No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC59340.10098555365406373No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCACC63950.039.13500694
GTACTAG67950.036.4480361
CAACGCA109200.033.080735
TAGGCAT84050.032.751515
GCATCGT73550.031.3421388
GTATCAA131100.031.1255991
ATCAACG116550.031.1197363
CTAGGCA94700.029.7639984
TATTCTC33150.029.4748215
CTGTGCG92850.028.8732179
GAACCGT8050.028.5754346
GGTATCA84400.028.1703591
TAGTACT96400.028.069554
ACGCAGA132800.026.9393257
ACTGTGC102650.026.939298
TGAACCG8550.026.921635
TCTAACG35950.026.7921852
CTAACGC36850.026.5189743
TATCAAC153250.026.3970762
AACGCAG145650.026.3332376
Produced by FastQC (version 0.11.5)