##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1146472_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5876088
Sequences flagged as poor quality	0
Sequence length	100
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.52854433085413	31.0	31.0	34.0	27.0	34.0
2	30.634350438591117	31.0	31.0	34.0	27.0	34.0
3	30.84546232119056	31.0	31.0	34.0	27.0	34.0
4	34.30535281295992	37.0	35.0	37.0	30.0	37.0
5	34.54762079805476	37.0	35.0	37.0	30.0	37.0
6	34.6674200590597	37.0	35.0	37.0	31.0	37.0
7	34.56755617002332	37.0	35.0	37.0	30.0	37.0
8	34.492468288425904	37.0	35.0	37.0	30.0	37.0
9	35.88939546174257	39.0	35.0	39.0	30.0	39.0
10-11	35.809654058958955	39.0	35.0	39.0	30.0	39.0
12-13	35.77874497454769	39.0	35.0	39.0	30.0	39.0
14-15	36.802260364378476	40.0	36.0	41.0	29.5	41.0
16-17	36.64228786226482	40.0	36.0	41.0	28.5	41.0
18-19	36.60102988586965	40.0	36.0	41.0	28.5	41.0
20-21	36.93425413302184	40.0	36.0	41.0	30.0	41.0
22-23	36.64996431299191	40.0	36.0	41.0	28.5	41.0
24-25	36.766946733949524	40.0	36.0	41.0	29.5	41.0
26-27	36.83594450934022	40.0	36.0	41.0	30.0	41.0
28-29	36.67313202252927	40.0	35.5	41.0	29.5	41.0
30-31	36.618909978883906	40.0	35.0	41.0	29.5	41.0
32-33	36.721199376183606	40.0	35.5	41.0	30.0	41.0
34-35	36.76943486891278	40.0	35.0	41.0	30.0	41.0
36-37	36.70067968689373	40.0	35.0	41.0	30.0	41.0
38-39	36.53190753780406	39.0	35.0	41.0	29.0	41.0
40-41	36.49317564679086	39.0	35.0	41.0	29.0	41.0
42-43	36.34072030575444	39.0	35.0	41.0	29.0	41.0
44-45	36.154079721066125	39.0	35.0	41.0	28.0	41.0
46-47	36.02593800501286	39.0	35.0	41.0	28.0	41.0
48-49	35.89111956798469	38.0	34.0	40.5	28.0	41.0
50-51	35.6646316392811	38.0	34.0	40.0	27.5	41.0
52-53	35.473652538900026	38.0	34.0	40.0	27.0	41.0
54-55	35.27265980359722	38.0	34.0	40.0	27.0	41.0
56-57	35.117314955119795	37.0	33.5	40.0	27.0	41.0
58-59	34.84452045306333	37.0	33.0	40.0	26.5	41.0
60-61	34.5975283215636	36.0	33.0	40.0	26.0	41.0
62-63	34.32924804734034	36.0	33.0	39.0	26.0	41.0
64-65	34.038254022063654	35.0	33.0	39.0	26.0	41.0
66-67	33.7576638573146	35.0	32.0	39.0	26.0	41.0
68-69	33.47468179509906	35.0	32.0	38.0	25.0	40.0
70-71	33.425650619936256	35.0	32.0	37.5	26.0	40.0
72-73	33.22571428474183	35.0	32.0	37.0	25.5	40.0
74-75	32.96580046112311	35.0	32.0	36.5	25.0	39.5
76-77	32.737393568646354	35.0	32.0	36.0	25.0	39.0
78-79	32.52100181277068	35.0	32.0	36.0	25.0	39.0
80-81	32.31102018894203	35.0	31.5	35.5	24.5	38.0
82-83	32.0695837094339	35.0	31.0	35.0	24.0	37.0
84-85	31.893913093200783	35.0	31.0	35.0	24.0	37.0
86-87	31.728453777411094	35.0	31.0	35.0	24.0	36.0
88-89	31.733796022115392	35.0	32.0	35.0	24.0	36.0
90-91	31.65587930269254	35.0	32.0	35.0	24.0	36.0
92-93	31.507112044611993	35.0	31.0	35.0	24.0	36.0
94-95	31.49303022691287	35.0	31.0	35.0	24.0	35.0
96-97	31.401137797800168	35.0	31.0	35.0	23.5	35.0
98-99	31.325400419462746	35.0	31.0	35.0	23.0	35.0
100	30.841875070625218	34.0	30.0	35.0	20.0	35.0
>>END_MODULE
>>Per tile sequence quality	warn
#Tile	Base	Mean
1101	1	0.8508397825107181
1101	2	0.9855022928442878
1101	3	0.9757367496976137
1101	4	1.0538539124370558
1101	5	0.9384419321134772
1101	6	0.7005889349913801
1101	7	0.7070598800491865
1101	8	0.7730148416376537
1101	9	0.9843706321149384
1101	10-11	1.0048281383946716
1101	12-13	0.867837714991353
1101	14-15	1.0864440845396928
1101	16-17	1.259067037205412
1101	18-19	1.3772289406589522
1101	20-21	1.009932874762356
1101	22-23	1.3326196991326356
1101	24-25	1.1502024727846276
1101	26-27	1.0850889636606382
1101	28-29	1.317755571517317
1101	30-31	1.3276316103535564
1101	32-33	1.1754032699845567
1101	34-35	1.1040930607914987
1101	36-37	1.013804777547712
1101	38-39	1.0929136363785616
1101	40-41	1.0535054259950272
1101	42-43	1.1199730788183189
1101	44-45	1.087268520608383
1101	46-47	1.105829198670456
1101	48-49	1.0726984164928481
1101	50-51	1.077171331577162
1101	52-53	1.0639843398959385
1101	54-55	1.07204433498584
1101	56-57	0.9666838062890761
1101	58-59	0.8984617643571227
1101	60-61	0.9114081072031865
1101	62-63	0.868242962666514
1101	64-65	0.9022143685932278
1101	66-67	0.9504981849236032
1101	68-69	0.9402557383860426
1101	70-71	0.8591202368948174
1101	72-73	0.8804198567917183
1101	74-75	0.8422360448685282
1101	76-77	0.8091577798967364
1101	78-79	0.8586045169628704
1101	80-81	0.7982654283384818
1101	82-83	0.7922847025395967
1101	84-85	0.8327984638597812
1101	86-87	0.8700345812005068
1101	88-89	0.858378067979352
1101	90-91	0.8935041333479425
1101	92-93	0.9443250795515645
1101	94-95	0.8877607190763044
1101	96-97	0.8819664374568248
1101	98-99	0.8808182693004198
1101	100	0.9742536379762612
1102	1	0.8407633978296758
1102	2	0.9713696644303269
1102	3	0.9050877809237114
1102	4	0.9976040495390137
1102	5	0.9008154144252529
1102	6	0.7087583228171113
1102	7	0.7517768479826827
1102	8	0.781060923007729
1102	9	0.9221408692172446
1102	10-11	0.9257261330743844
1102	12-13	0.8258186881642189
1102	14-15	1.0909720138540706
1102	16-17	1.2663922364724414
1102	18-19	1.300582059734623
1102	20-21	1.0603117636442008
1102	22-23	1.3456308030508026
1102	24-25	1.1661522749434496
1102	26-27	1.1165268816997482
1102	28-29	1.2505120569884127
1102	30-31	1.2871154342722306
1102	32-33	1.144464262929354
1102	34-35	1.0608535462274915
1102	36-37	0.9994888712342913
1102	38-39	1.1004381636144487
1102	40-41	0.9594039666452119
1102	42-43	1.0283425014496714
1102	44-45	1.0573609651082947
1102	46-47	1.015800758239969
1102	48-49	0.9513773982871712
1102	50-51	1.0922710894002563
1102	52-53	1.0217122350384074
1102	54-55	1.0060286507654155
1102	56-57	0.8902014355293062
1102	58-59	0.8763424837024587
1102	60-61	0.9018835000216328
1102	62-63	0.9001800902671278
1102	64-65	0.8600266393282894
1102	66-67	0.9102185005822747
1102	68-69	0.869109424637287
1102	70-71	0.8151322675003598
1102	72-73	0.8168043099413609
1102	74-75	0.868204112664408
1102	76-77	0.8777159072277811
1102	78-79	0.9171117483904894
1102	80-81	0.9192868269685874
1102	82-83	0.8852132454857795
1102	84-85	0.8447286193687447
1102	86-87	0.8286367481183063
1102	88-89	0.8232759507838594
1102	90-91	0.8698660371859752
1102	92-93	0.9676531181087817
1102	94-95	0.9200625257023347
1102	96-97	0.9297820132631216
1102	98-99	0.9757614798407701
1102	100	1.0224700450713406
1103	1	0.7180768930641896
1103	2	0.8646878671059213
1103	3	0.8380354146302125
1103	4	0.8484153210732615
1103	5	0.7985976727943722
1103	6	0.5727760285390744
1103	7	0.6931291156686612
1103	8	0.6831200658867473
1103	9	0.8305768373884703
1103	10-11	0.8429142697862275
1103	12-13	0.7536445805284657
1103	14-15	0.9550589750895284
1103	16-17	1.1740385346655344
1103	18-19	1.2188824303241432
1103	20-21	0.9375728519142257
1103	22-23	1.1885224588431598
1103	24-25	1.087803439998254
1103	26-27	0.9737812849393066
1103	28-29	1.1684570851526246
1103	30-31	1.1846227917640562
1103	32-33	0.985293307023781
1103	34-35	1.0141553511796744
1103	36-37	0.9520546399502408
1103	38-39	0.9939901973183183
1103	40-41	0.8910026407345057
1103	42-43	0.9284041122664348
1103	44-45	0.9757624209486266
1103	46-47	0.9009336723216208
1103	48-49	0.9039869986121474
1103	50-51	0.9492873225662919
1103	52-53	0.9358532621073437
1103	54-55	0.9183352104772311
1103	56-57	0.818594993461204
1103	58-59	0.8423147721904769
1103	60-61	0.8316066912939348
1103	62-63	0.7820292898311223
1103	64-65	0.7467913909792898
1103	66-67	0.867047822423956
1103	68-69	0.856206107207548
1103	70-71	0.7566813030327353
1103	72-73	0.7220893516750664
1103	74-75	0.7309521521018567
1103	76-77	0.7512628452729189
1103	78-79	0.732772148359814
1103	80-81	0.7744354395709507
1103	82-83	0.7033715214078384
1103	84-85	0.6855333696442969
1103	86-87	0.6931688724332759
1103	88-89	0.731065250130186
1103	90-91	0.6696769140363124
1103	92-93	0.7281118458414859
1103	94-95	0.7322140703027387
1103	96-97	0.7091475566146244
1103	98-99	0.7755165836427551
1103	100	0.7750899309882939
1104	1	0.5775874947830175
1104	2	0.7100307738408098
1104	3	0.6961913889499343
1104	4	0.7925489357580702
1104	5	0.6967442496496332
1104	6	0.5004895550475723
1104	7	0.5162604050665962
1104	8	0.52984109487452
1104	9	0.6023714061630301
1104	10-11	0.6445100032829743
1104	12-13	0.5669724990732163
1104	14-15	0.728793407795969
1104	16-17	0.8672643505389459
1104	18-19	0.8546580095799214
1104	20-21	0.7202663494176704
1104	22-23	0.9747921414332907
1104	24-25	0.7960199326485622
1104	26-27	0.7077762953717937
1104	28-29	0.9412981946206429
1104	30-31	0.9262098958514713
1104	32-33	0.7913360736923707
1104	34-35	0.6292934861908606
1104	36-37	0.6781721240702581
1104	38-39	0.8004271110300252
1104	40-41	0.6287239170861199
1104	42-43	0.6570343709976072
1104	44-45	0.678072533960183
1104	46-47	0.6458029538128542
1104	48-49	0.6121725782365743
1104	50-51	0.6597904369522709
1104	52-53	0.7523472493508763
1104	54-55	0.6964314687500277
1104	56-57	0.6355946448190437
1104	58-59	0.6107707103173823
1104	60-61	0.6126947925947732
1104	62-63	0.5859383600241941
1104	64-65	0.5477174495855408
1104	66-67	0.6615196121351374
1104	68-69	0.627103428983844
1104	70-71	0.5245965196226621
1104	72-73	0.47984017572724014
1104	74-75	0.5077899031653885
1104	76-77	0.5252164241408366
1104	78-79	0.5599662465861144
1104	80-81	0.6205333421018651
1104	82-83	0.5073881968385692
1104	84-85	0.4793716331460942
1104	86-87	0.5111405488265106
1104	88-89	0.4384597150932663
1104	90-91	0.5002612600306442
1104	92-93	0.5785208930346464
1104	94-95	0.4904744762658986
1104	96-97	0.5915522296146207
1104	98-99	0.5922198420533888
1104	100	0.6205196255662848
1105	1	0.45384469631401814
1105	2	0.6240741403233088
1105	3	0.6198456160233405
1105	4	0.661881693767981
1105	5	0.5394543662562086
1105	6	0.27253752589312086
1105	7	0.3170013174573256
1105	8	0.412632561739116
1105	9	0.3909016945438921
1105	10-11	0.47388522394939514
1105	12-13	0.4043618447582418
1105	14-15	0.5797636712106424
1105	16-17	0.7128135165715435
1105	18-19	0.7481596309324772
1105	20-21	0.6289097610618768
1105	22-23	0.8064348052162416
1105	24-25	0.6764021853416153
1105	26-27	0.5149089799273483
1105	28-29	0.7004911673883711
1105	30-31	0.6991687184982638
1105	32-33	0.601683531748364
1105	34-35	0.4883430601881287
1105	36-37	0.41639984974634814
1105	38-39	0.49782757845071046
1105	40-41	0.4140496007487968
1105	42-43	0.47844652955840417
1105	44-45	0.5168278229255421
1105	46-47	0.39978138924686135
1105	48-49	0.46478045359737763
1105	50-51	0.4849003447377527
1105	52-53	0.4905227875105922
1105	54-55	0.4200827777735796
1105	56-57	0.4178864089636676
1105	58-59	0.41950258681580976
1105	60-61	0.476429049996959
1105	62-63	0.4193134043054556
1105	64-65	0.4266503371024086
1105	66-67	0.503847517481411
1105	68-69	0.42341734603961356
1105	70-71	0.39537916367770265
1105	72-73	0.387935242595276
1105	74-75	0.4013384974259466
1105	76-77	0.36314705327652064
1105	78-79	0.403159475053144
1105	80-81	0.5185112874455484
1105	82-83	0.4812849209576697
1105	84-85	0.4159934074210341
1105	86-87	0.4361644422093818
1105	88-89	0.43900715426776316
1105	90-91	0.44947955709107745
1105	92-93	0.49833821601097483
1105	94-95	0.43245673180005895
1105	96-97	0.4841212765635561
1105	98-99	0.5011430786974671
1105	100	0.5269626737268354
1106	1	0.627039786124044
1106	2	0.6728542714001655
1106	3	0.661177387316016
1106	4	0.7799287334230414
1106	5	0.6554618605411591
1106	6	0.4061580523944244
1106	7	0.4490094318782667
1106	8	0.44684973511831316
1106	9	0.6756716518802932
1106	10-11	0.662062653046938
1106	12-13	0.5355946721834641
1106	14-15	0.7748601213708639
1106	16-17	0.8852572765262607
1106	18-19	0.8783068401011249
1106	20-21	0.7212635427944747
1106	22-23	1.0498434177935891
1106	24-25	0.8073699923857163
1106	26-27	0.6863733930432332
1106	28-29	0.8902906908782171
1106	30-31	0.8248985117911403
1106	32-33	0.7072267869650375
1106	34-35	0.7009379344131474
1106	36-37	0.5780853910709496
1106	38-39	0.7402079646023694
1106	40-41	0.6532501518507843
1106	42-43	0.6346131823305114
1106	44-45	0.691415878579825
1106	46-47	0.6508578663478986
1106	48-49	0.6391078181217935
1106	50-51	0.7123890426130544
1106	52-53	0.705057458816583
1106	54-55	0.7577704310707603
1106	56-57	0.7111082348253888
1106	58-59	0.7084813369200162
1106	60-61	0.644168639683393
1106	62-63	0.6325723798232445
1106	64-65	0.6883890872651293
1106	66-67	0.6947364619485228
1106	68-69	0.6773199466617967
1106	70-71	0.6584984184614484
1106	72-73	0.5992329502859803
1106	74-75	0.5764785033640436
1106	76-77	0.5952933819690713
1106	78-79	0.5661989019538822
1106	80-81	0.5792479622003128
1106	82-83	0.6365405588567583
1106	84-85	0.5778669494067188
1106	86-87	0.5277663675561186
1106	88-89	0.512250450875591
1106	90-91	0.5452276765725621
1106	92-93	0.5448085160310079
1106	94-95	0.5918223794838369
1106	96-97	0.5769096215171245
1106	98-99	0.6252712537864298
1106	100	0.5694916585027876
1107	1	0.4750632443491263
1107	2	0.5937270471964133
1107	3	0.5257652508379493
1107	4	0.6529584943924149
1107	5	0.5737523952950312
1107	6	0.2864997317161624
1107	7	0.2671229531401309
1107	8	0.34054480922470276
1107	9	0.43343675533805026
1107	10-11	0.4943000905196868
1107	12-13	0.3808962256859729
1107	14-15	0.604763851837177
1107	16-17	0.6517204062596562
1107	18-19	0.773026254525135
1107	20-21	0.513273360492633
1107	22-23	0.8306492485874557
1107	24-25	0.4806334536300483
1107	26-27	0.46605986111833175
1107	28-29	0.616429883471298
1107	30-31	0.671234706996195
1107	32-33	0.5308023230074568
1107	34-35	0.377445719425225
1107	36-37	0.30025562804830486
1107	38-39	0.3917837673375004
1107	40-41	0.2722365900716994
1107	42-43	0.42849635879230163
1107	44-45	0.40146452374937525
1107	46-47	0.38807623925873
1107	48-49	0.3860290981581116
1107	50-51	0.4126835478916675
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2216	90-91	-1.5668679461207908
2216	92-93	-1.4256857283071973
2216	94-95	-1.4203203008551348
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2216	98-99	-1.362298352314376
2216	100	-1.3461462757444416
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	24141.0
1	4242.0
2	661.0
3	609.0
4	581.0
5	340.0
6	284.0
7	352.0
8	622.0
9	1156.0
10	2004.0
11	3658.0
12	6229.0
13	9170.0
14	12127.0
15	15327.0
16	18714.0
17	22597.0
18	26249.0
19	29969.0
20	34270.0
21	39065.0
22	45162.0
23	51912.0
24	59588.0
25	67086.0
26	76456.0
27	88318.0
28	104185.0
29	124625.0
30	147862.0
31	178561.0
32	219318.0
33	280341.0
34	387138.0
35	522061.0
36	773910.0
37	1183586.0
38	962893.0
39	350393.0
40	326.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.36069569641838	21.433919841580888	12.488792398083019	23.71659206391771
2	15.374102166936563	19.715633701717618	41.26166133797533	23.648602793370486
3	18.358531871502333	27.128132745399743	28.877671804872445	25.635663578225483
4	11.915776313575725	16.873940775048318	40.81818672998877	30.392096181387185
5	12.850982295772384	38.77143804545874	35.389687860007044	12.987891798761833
6	29.19373681124086	39.0595058342222	18.46743519112809	13.279322163408844
7	26.525118450986096	31.770572840942137	24.237278278569352	17.46703042950242
8	25.870531285279323	35.15801717354863	20.196007663097397	18.775443878074647
9	25.99453309601674	14.889084599808806	21.948962561048464	37.167419743126
10-11	25.515540471407018	25.9228689448248	27.81071446781324	20.750876115954945
12-13	25.746752519031645	23.75782494870913	27.746912666668607	22.74850986559062
14-15	23.162215270167728	24.963672806317415	25.14704963989327	26.727062283621585
16-17	21.961203845138762	27.51201659331451	26.618342217007463	23.90843734453927
18-19	21.260671138459234	28.393387596314966	28.217249489119073	22.128691776106727
20-21	23.259255368411814	27.482107432653873	26.915412155901553	22.343225043032756
22-23	23.771498440570163	27.094673309903616	26.635109495311877	22.49871875421434
24-25	23.166050018439137	27.149853346620116	27.417890232423815	22.26620640251693
26-27	23.06515955513308	27.31929312708081	26.31006848646068	23.305478831325427
28-29	22.031166444221906	27.94683427631437	27.07228593725997	22.949713342203754
30-31	22.98687071840803	27.492096196945127	26.952804538165655	22.568228546481187
32-33	23.42420583186756	27.78739279126897	25.865126675391032	22.923274701472433
34-35	23.37530315187266	27.609116495097684	26.952711259288755	22.062869093740904
36-37	22.991177429415586	27.12877564693289	26.5877503942391	23.29229652941242
38-39	23.174491953126136	27.132205547556165	27.314796990860163	22.37850550845754
40-41	22.72713116426026	26.202640794216414	27.953293788613255	23.116934252910067
42-43	22.68779255357832	26.510968914350837	27.203035926499165	23.598202605571682
44-45	22.93078237303961	27.506309846423683	27.008701115001223	22.554206665535485
46-47	22.83823127009576	27.20643310790657	26.53349836906569	23.421837252931976
48-49	23.618932962132998	26.76999850218509	26.818542760391615	22.792525775290294
50-51	23.199872217079022	26.507932148774703	26.790695493795518	23.50150014035075
52-53	22.547088500931398	27.280417984631413	26.633448608464487	23.539044905972705
54-55	22.519845802227618	27.178435377536946	26.62545816925993	23.676260650975507
56-57	23.82449783015406	26.510718346041024	26.31625771935633	23.34852610444859
58-59	22.539742261422173	26.976034403241783	27.36308627219594	23.12113706314011
60-61	22.588903289915535	27.74926039925737	26.480405531237487	23.18143077958961
62-63	23.089936273042213	26.882562764637953	26.03388221205252	23.993618750267313
64-65	22.182784768802836	27.057217510240726	26.60714918170872	24.152848539247714
66-67	23.04117657727144	26.64112435886161	26.55195783106763	23.76574123279932
68-69	22.338067135839992	26.75457627101241	26.940182925060586	23.96717366808701
70-71	22.315576912021157	26.385234928726103	27.090607983990406	24.20858017526233
72-73	22.99532352592166	26.504813660119297	27.48548638418919	23.014376429769857
74-75	23.252161514817164	26.805572701438603	26.94336143775266	22.998904345991573
76-77	22.87703801006335	26.798539042429	26.534162419426764	23.790260528080882
78-79	22.69776465090752	26.733067896128492	26.66672713462075	23.902440318343242
80-81	22.24419419624507	27.12764697010746	27.416030554921328	23.212128278726144
82-83	23.22801225661979	25.981842279213218	28.52746306294191	22.26268240122508
84-85	23.506971138257356	26.625071800593776	26.91488355085826	22.953073510290604
86-87	21.936354111448743	27.35601118127801	27.5244014409373	23.183233266335947
88-89	23.160769671934716	25.968266069717583	27.330461311822283	23.540502946525415
90-91	22.79739730892811	26.00370559277094	27.511668814237844	23.687228284063096
92-93	23.129045555435386	26.281377024880324	27.3962770586683	23.193300361015986
94-95	21.89616884073989	26.308249254187377	28.113317570545988	23.682264334526742
96-97	22.295727621284122	26.21595152050779	27.55278902226835	23.935531835939734
98-99	21.576782548815352	26.977581365800845	27.482743976953657	23.962892108430147
100	22.490842049660266	26.174910399198886	26.61984311471828	24.714404436422573
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	20372.0
1	11958.5
2	3223.0
3	2273.0
4	1227.5
5	668.5
6	488.5
7	455.0
8	458.5
9	441.5
10	408.5
11	388.0
12	379.0
13	373.5
14	395.0
15	539.0
16	618.5
17	714.0
18	953.5
19	1160.5
20	1436.0
21	1855.0
22	2563.5
23	3635.0
24	5116.5
25	6969.5
26	9924.0
27	13588.0
28	19589.0
29	31893.5
30	48676.5
31	64544.5
32	85671.0
33	105770.0
34	120830.5
35	138497.5
36	155098.0
37	184773.5
38	198056.5
39	183639.5
40	169154.0
41	155459.5
42	152121.0
43	161207.5
44	176998.5
45	212371.5
46	247192.0
47	268857.0
48	281535.0
49	274282.5
50	260455.0
51	267920.0
52	271399.0
53	257694.5
54	242690.5
55	207930.0
56	180770.0
57	147735.0
58	113658.5
59	108553.0
60	89802.5
61	61029.5
62	45365.5
63	38300.0
64	27858.5
65	12768.5
66	6528.5
67	4346.0
68	3091.0
69	2318.5
70	1811.0
71	1377.0
72	976.0
73	796.0
74	654.5
75	486.5
76	346.5
77	228.5
78	149.5
79	96.5
80	65.0
81	44.5
82	35.5
83	27.5
84	25.0
85	23.0
86	18.0
87	15.0
88	12.0
89	11.0
90	8.0
91	7.5
92	6.0
93	5.5
94	4.0
95	4.0
96	5.5
97	2.5
98	1.0
99	2.0
100	7.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.9973982690524716
2	0.4477298501996566
3	0.48096624829308204
4	0.46752192955585414
5	0.43409833208760656
6	0.48976461890972367
7	0.506935907018411
8	0.5086377195167942
9	0.523001017003149
10-11	0.5367856982400536
12-13	0.5357475926160398
14-15	0.5325481851190792
16-17	0.5371856241771736
18-19	0.5365219173028042
20-21	0.5345818510546473
22-23	0.4976014654647786
24-25	0.5306847004333495
26-27	0.5125008338881242
28-29	0.5180657607578376
30-31	0.5214778948170959
32-33	0.5237072691899781
34-35	0.5352455579290167
36-37	0.5539910225987086
38-39	0.5427590601093789
40-41	0.5486643494787689
42-43	0.5458223226064688
44-45	0.5554971266597777
46-47	0.5531741525994845
48-49	0.5259706798128279
50-51	0.5412019016733582
52-53	0.5339521804302454
54-55	0.542963277609185
56-57	0.5310165538705343
58-59	0.5364538448028688
60-61	0.5311612079328968
62-63	0.5248049382514353
64-65	0.5365984988652315
66-67	0.5344712332422523
68-69	0.5538974229112975
70-71	0.5376366044892452
72-73	0.5417634997978247
74-75	0.5383428566760743
76-77	0.5278596916860333
78-79	0.5577265010326599
80-81	0.5321312410569754
82-83	0.531756842307331
84-85	0.5407849576112543
86-87	0.8465070638833182
88-89	1.1135044267546708
90-91	1.1039998039512002
92-93	1.103616896139064
94-95	1.1086372430092946
96-97	1.1014726123911012
98-99	1.0975669527073115
100	1.0925295877120969
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	5876088.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.276517785319037
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.24389550509474	18.26074109615257
2	12.886602845765202	6.51456892046654
3	5.328757553813545	4.040773052478637
4	2.7966331475370936	2.8275658997093642
5	1.6968427220731317	2.144513762168534
6	1.0809943399698736	1.6394263596046632
7	0.7304826094372175	1.292483966851426
8	0.48008199994060174	0.9707840967928124
9	0.38478622983565863	0.8753450383788197
>10	1.858392521804871	8.452136402493599
>50	0.1858685528100714	3.331229334838219
>100	0.22918816391673796	13.00824955319232
>500	0.050454652539575685	8.899778024111793
>1k	0.04365102111635205	20.32306796050567
>5k	0.002694507476318028	4.639381841399268
>10k+	6.73626869079507E-4	2.779954690855885
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	25385	0.43200510271459514	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT	21467	0.36532808902793834	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	18150	0.308878968456565	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	18055	0.30726224658310086	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	15903	0.2706392416178927	No Hit
ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA	15389	0.26189192537620265	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	14732	0.25071101726182454	No Hit
CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC	13126	0.22337990853778908	No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG	10714	0.18233219107678442	No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT	10347	0.1760865392077178	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	9495	0.16158709672149227	No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT	9327	0.15872805172420834	No Hit
GTCTTAGCAGTAGTTTATAAGGAATATATCCCATTTTTAGTTATAATGAT	9307	0.1583876892245317	No Hit
TTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACAAATATCC	8730	0.1485682311088602	No Hit
GAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGGGAAGCTAT	8421	0.14330963048885584	No Hit
CCCTCAGGAAGCTAAGAAATACTATCTCGGCAATAGGATTGTAGCCCAGG	8274	0.14080796611623245	No Hit
GTCTAACGCCTACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACT	8236	0.14016127736684678	No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC	7825	0.1331668279984915	No Hit
TCCCTACTCATAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCAC	7726	0.1314820336250921	No Hit
CTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCCCAATTATC	7680	0.13069919987583578	No Hit
GTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACTTA	7468	0.12709135737926322	No Hit
AGGCTACTACGTGCGCGATATGACAAGTTAACAAGACGGCGCAGGTTGAT	7419	0.1262574692550554	No Hit
CCCCTATCCCCTACGAGGCTACCCGGATCGATGACGCGAATTGGGGACAT	7372	0.12545761738081526	No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA	7318	0.1245386386316883	No Hit
TACCAGTACTGATTGCCGAGCAAATCTGTGAATGGCGACTATGCGCCTAG	7181	0.1222071555089032	No Hit
ATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCTTCCCA	7078	0.12045428863556842	No Hit
GTCCAGTCATGACCTCGATCCATCGCGTATAGGGACGCCCCCTGCTCGCG	7001	0.1191438930118133	No Hit
ATGTGATAGATGCCTCTTTAAAATATCTAAGTGCTGGGGTTATGAGTAGG	6851	0.1165911742642384	No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT	6731	0.11454899926617845	No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA	6720	0.1143617998913563	No Hit
GGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATA	6686	0.11378318364190598	No Hit
GTCTCAGGGGATCTGCATATGTTTGCAGCATACTTTAGGTGGGCCTTGGC	6375	0.10849054677193398	No Hit
ATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCTGGGGAGATTA	6370	0.10840545614701483	No Hit
GAATATATCCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTC	6331	0.10774174927264533	No Hit
GAGTACATGGGGGGAATTCTGGACATTAATTAGGGCTGAAAGCCCTAACT	6260	0.10653346239879322	No Hit
GTGTACACCCTTTTGAGCAATGATTGCACAACCTGCGATCACCTTATACA	6207	0.10563150177465008	No Hit
CCATCATGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATATCC	6134	0.10438917865083028	No Hit
GTGCTAGCCCGTCGAGACTGAAAAGCTATAACCCGCAGACCCGAGCGAAA	6085	0.10355529052662248	No Hit
ATCCAAGATAGTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTG	6024	0.10251718490260867	No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC	5934	0.10098555365406373	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.701812498383278E-5	3.403624996766556E-5
2	0.0	0.0	0.0	5.1054374951498346E-5	3.403624996766556E-5
3	0.0	0.0	0.0	5.1054374951498346E-5	3.403624996766556E-5
4	0.0	0.0	0.0	8.50906249191639E-5	3.403624996766556E-5
5	0.0	0.0	0.0	1.3614499987066225E-4	5.1054374951498346E-5
6	0.0	0.0	0.0	1.3614499987066225E-4	5.1054374951498346E-5
7	0.0	0.0	0.0	1.3614499987066225E-4	8.50906249191639E-5
8	0.0	0.0	0.0	1.3614499987066225E-4	1.0210874990299669E-4
9	0.0	0.0	0.0	1.3614499987066225E-4	1.0210874990299669E-4
10-11	0.0	0.0	0.0	2.0421749980599339E-4	1.0210874990299669E-4
12-13	0.0	0.0	0.0	2.2123562478982615E-4	2.722899997413245E-4
14-15	0.0	0.0	0.0	2.2123562478982615E-4	3.063262497089901E-4
16-17	0.0	0.0	0.0	2.2974468728174255E-4	3.2334437469282287E-4
18-19	0.0	0.0	0.0	2.3825374977365894E-4	3.2334437469282287E-4
20-21	0.0	0.0	0.0	2.3825374977365894E-4	3.2334437469282287E-4
22-23	0.0	0.0	0.0	3.0632624970899E-4	3.2334437469282287E-4
24-25	0.0	0.0	0.0	5.190528120068999E-4	3.2334437469282287E-4
26-27	0.0	0.0	0.0	8.253790617158899E-4	3.91416874628154E-4
28-29	0.0	0.0	0.0	0.0017869031233024419	3.91416874628154E-4
30-31	0.0	0.0	0.0	0.006381796868937292	4.0843499961198677E-4
32-33	0.0	0.0	0.0	0.018141321232765746	4.424712495796523E-4
34-35	0.0	0.0	0.0	0.03665704121517581	4.765074995473179E-4
36-37	0.0	0.0	0.0	0.06843838962248353	4.765074995473179E-4
38-39	0.0	0.0	0.0	0.1207946511352451	5.105437495149834E-4
40-41	0.0	0.0	8.50906249191639E-6	0.1763588292074591	5.275618744988162E-4
42-43	0.0	0.0	1.701812498383278E-5	0.23747091602440262	5.44579999482649E-4
44-45	0.0	0.0	1.701812498383278E-5	0.314171605326537	5.701071869583982E-4
46-47	0.0	0.0	1.701812498383278E-5	0.41065586492237693	6.041434369260638E-4
48-49	0.0	0.0	1.701812498383278E-5	0.5244986120017263	6.126524994179802E-4
50-51	0.0	0.0	1.701812498383278E-5	0.6470291118853224	7.147612493209768E-4
52-53	0.0	0.0	1.701812498383278E-5	0.768164125520244	7.487974992886424E-4
54-55	0.0	0.0	1.701812498383278E-5	0.9032710197668925	7.658156242724752E-4
56-57	0.0	0.0	1.701812498383278E-5	1.070542170232985	7.743246867643915E-4
58-59	0.0	0.0	1.701812498383278E-5	1.275185123163574	7.82833749256308E-4
60-61	0.0	0.0	1.701812498383278E-5	1.4906345854588972	7.998518742401408E-4
62-63	0.0	0.0	1.701812498383278E-5	1.7170011749313487	8.168699992239735E-4
64-65	0.0	0.0	1.701812498383278E-5	1.957160954703197	8.168699992239735E-4
66-67	0.0	0.0	1.701812498383278E-5	2.2328205431913206	8.2537906171589E-4
68-69	0.0	0.0	1.701812498383278E-5	2.5431971066464625	8.50906249191639E-4
70-71	0.0	0.0	1.701812498383278E-5	2.8884352991309865	8.594153116835554E-4
72-73	0.0	0.0	1.701812498383278E-5	3.2535932069090867	9.274878116188865E-4
74-75	0.0	0.0	1.701812498383278E-5	3.6165302493767966	9.870512490623012E-4
76-77	0.0	0.0	1.701812498383278E-5	4.032504278356621	9.870512490623012E-4
78-79	0.0	0.0	1.701812498383278E-5	4.474898946373846	9.870512490623012E-4
80-81	0.0	0.0	1.701812498383278E-5	4.961268449349295	9.955603115542177E-4
82-83	0.0	0.0	1.701812498383278E-5	5.464647568246085	0.001046614686505716
84-85	0.0	0.0	1.701812498383278E-5	5.959466570276007	0.0010551237489976324
86-87	0.0	0.0	1.701812498383278E-5	6.4877346969616525	0.0011402143739167965
88	0.0	0.0	1.701812498383278E-5	6.9148896340558546	0.0012082868738521274
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGCACC	6395	0.0	39.135006	94
GTACTAG	6795	0.0	36.448036	1
CAACGCA	10920	0.0	33.08073	5
TAGGCAT	8405	0.0	32.75151	5
GCATCGT	7355	0.0	31.342138	8
GTATCAA	13110	0.0	31.125599	1
ATCAACG	11655	0.0	31.119736	3
CTAGGCA	9470	0.0	29.763998	4
TATTCTC	3315	0.0	29.474821	5
CTGTGCG	9285	0.0	28.873217	9
GAACCGT	805	0.0	28.575434	6
GGTATCA	8440	0.0	28.170359	1
TAGTACT	9640	0.0	28.06955	4
ACGCAGA	13280	0.0	26.939325	7
ACTGTGC	10265	0.0	26.93929	8
TGAACCG	855	0.0	26.92163	5
TCTAACG	3595	0.0	26.792185	2
CTAACGC	3685	0.0	26.518974	3
TATCAAC	15325	0.0	26.397076	2
AACGCAG	14565	0.0	26.333237	6
>>END_MODULE